AT5G09460

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0020850
Physical Location & Seq
Reverse (-)
2943738 .. 2946839
3102 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G09460.1

Sequence Viewer

Length: 981 bp
ATGCCTCTTGATACCAAACAGCAGAAATGGTTGCCATTAGGCTTAAATCCTCAAGCTTGTGTCCAGGACAAGGCGACTGAGTATTTCCGTCCTGGAATTCCTTTTCCGGAACTCGGTAAAGTTTATGCAGCTGAGCATCAGTTTCGCTATTTGCAGCCACCGTTCCAAGCCTTATTGTCTAGATATGATCAGCAGTCTTGTGGAAAACAAGTTTCATGTTTGAATGGGCGATCTAGCAACGGTGCTGCTCCAGAGGGGGCACTCAAGTCTTCTCGGAAAAGATTTATAGTATTCGATCAGTCGGGAGAGCAGACTCGTTTGTTACAATGTGGATTTCCTCTGCGGTTTCCTTCTTCTATGGATGCAGAGCGAGGGAACATTCTCGGTGCCCTACACCCAGAGAAAGGGTTTAGTAAAGATCATGCCATTCAAGAAAAGATATTGCAACATGAAGATCATGAAAATGGCGAAGAAGACTCGGAAATGCACGAAGACACTGAGGAAATCAACGCGTTACTGTATTCTGATGATGACGATAATGATGATTGGGAAAGTGATGATGAAGTAATGAGCACTGGTCACTCTCCATTCACAGTTGAACAACAAGCGTGCAACATAACAACAGAAGAGCTGGATGAAACTGAAAGCACTGTTGATGGTCCACTTCTTAAAAGACAGAAACTACTGGACCATTCGTACAGAGACTCATCACCATCCCTTGTGGGCACCACTAAAGTCAAAGGCTTATCAGATGAAAACCTTCCTGAATCAAACATTTCAAGCAAACAAGAAACGGGTTCTGGTTTGAGCGACGAGCAGTCAAGAAAAGACAAGATTCACACCGCTCTGAGAATCCTGGAGAGTGTAGTTCCAGGGGCAAAGGGAAAAGAAGCTCTTTTACTACTAGACGAAGCCATTGATTACCTCAAGTTGCTGAAGCAAAGCTTAAACTCATCAAAGGGTTTGAATAACCATTGGTGA

Protein Analysis

326

Amino Acids

36.44

Weight (kDa)

4.87

Isoelectric Point (pI)

66.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_SAC51 PF23173 263 - 325 1.4e-35 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09460 AT5G64340
malus_domestica MD04G1047600.v1.1
prunus_persica Prupe.8G067600_v2.0.a1
pyrus_communis pycom04g04160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 386, 725
AccBSI CCGCTC 1 cut(s) 845
AccII CGCG 1 cut(s) 512
AccIII TCCGGA 1 cut(s) 106
AciI CCGC 2 cut(s) 343, 843
AcsI RAATTY 1 cut(s) 96
AcuI CTGAAG 1 cut(s) 956
AfaI GTAC 1 cut(s) 698
AfiI CCNNNNNNNGG 3 cut(s) 70, 113, 404
AflIII ACRYGT 1 cut(s) 510
AgsI TTSAA 5 cut(s) 223, 431, 599, 780, 967
AhdI GACNNNNNGTC 1 cut(s) 817
AjnI CCWGG 4 cut(s) 63, 91, 855, 871
AluBI AGCT 5 cut(s) 56, 131, 631, 893, 945
AluI AGCT 5 cut(s) 56, 131, 631, 893, 945
Alw21I GWGCWC 1 cut(s) 575
Alw26I GTCTC 1 cut(s) 696
Aor13HI TCCGGA 1 cut(s) 106
ApeKI GCWGC 3 cut(s) 128, 154, 245
ApoI RAATTY 1 cut(s) 96
Asp700I GAANNNNTTC 1 cut(s) 759
AspS9I GGNCC 2 cut(s) 659, 688
AsuHPI GGTGA 1 cut(s) 702
AvaII GGWCC 2 cut(s) 659, 688
BaeGI GKGCMC 3 cut(s) 262, 391, 728
BanI GGYRCC 2 cut(s) 386, 725
BarI GAAGNNNNNNTAC 2 cut(s) 882, 914
BbsI GAAGAC 3 cut(s) 261, 480, 498
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 3 cut(s) 140, 166, 232
BccI CCATC 2 cut(s) 650, 721
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 4 cut(s) 65, 93, 857, 873
BclI TGATCA 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 696
BfaI CTAG 3 cut(s) 180, 234, 905
BisI GCNGC 3 cut(s) 129, 155, 246
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 3 cut(s) 130, 156, 247
Bme1390I CCNGG 4 cut(s) 65, 93, 857, 873
Bme18I GGWCC 2 cut(s) 659, 688
BmeRI GACNNNNNGTC 1 cut(s) 817
BmgT120I GGNCC 2 cut(s) 659, 688
BmiI GGNNCC 2 cut(s) 388, 727
BmrFI CCNGG 4 cut(s) 65, 93, 857, 873
BmsI GCATC 2 cut(s) 145, 352
BpiI GAAGAC 3 cut(s) 261, 480, 498
BpmI CTGGAG 2 cut(s) 234, 878
Bpu1102I GCTNAGC 1 cut(s) 132
BpuEI CTTGAG 3 cut(s) 36, 248, 911
BsaJI CCNNGG 1 cut(s) 872
BsaWI WCCGGW 1 cut(s) 106
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 113, 404
Bse1I ACTGG 2 cut(s) 580, 690
BseAI TCCGGA 1 cut(s) 106
BseBI CCWGG 4 cut(s) 65, 93, 857, 873
BseDI CCNNGG 1 cut(s) 872
BseGI GGATG 3 cut(s) 367, 640, 713
BseLI CCNNNNNNNGG 3 cut(s) 70, 113, 404
BseMII CTCAG 4 cut(s) 69, 123, 489, 839
BseNI ACTGG 2 cut(s) 580, 690
BseSI GKGCMC 3 cut(s) 262, 391, 728
BseXI GCAGC 3 cut(s) 140, 166, 232
Bsh1236I CGCG 1 cut(s) 512
BshNI GGYRCC 2 cut(s) 386, 725
BsiHKAI GWGCWC 1 cut(s) 575
BsiSI CCGG 1 cut(s) 107
BslI CCNNNNNNNGG 3 cut(s) 70, 113, 404
BsmAI GTCTC 1 cut(s) 696
Bsp1286I GDGCHC 4 cut(s) 262, 391, 575, 728
Bsp13I TCCGGA 1 cut(s) 106
Bsp143I GATC 5 cut(s) 187, 230, 295, 418, 454
Bsp1720I GCTNAGC 1 cut(s) 132
BspACI CCGC 2 cut(s) 343, 843
BspCNI CTCAG 4 cut(s) 70, 124, 490, 840
BspEI TCCGGA 1 cut(s) 106
BspFNI CGCG 1 cut(s) 512
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 2 cut(s) 388, 727
BspQI GCTCTTC 1 cut(s) 621
BspT107I GGYRCC 2 cut(s) 386, 725
BsrBI CCGCTC 1 cut(s) 845
BsrI ACTGG 2 cut(s) 580, 690
BssECI CCNNGG 1 cut(s) 872
BssMI GATC 5 cut(s) 187, 230, 295, 418, 454
Bst2UI CCWGG 4 cut(s) 65, 93, 857, 873
Bst4CI ACNGT 5 cut(s) 162, 242, 519, 595, 652
Bst6I CTCTTC 1 cut(s) 621
BstC8I GCNNGC 1 cut(s) 610
BstDEI CTNAG 4 cut(s) 78, 132, 498, 848
BstF5I GGATG 3 cut(s) 367, 640, 713
BstFNI CGCG 1 cut(s) 512
BstKTI GATC 5 cut(s) 190, 233, 298, 421, 457
BstMAI GTCTC 1 cut(s) 696
BstMBI GATC 5 cut(s) 187, 230, 295, 418, 454
BstNI CCWGG 4 cut(s) 65, 93, 857, 873
BstSCI CCNGG 4 cut(s) 63, 91, 855, 871
BstSLI GKGCMC 3 cut(s) 262, 391, 728
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 3 cut(s) 140, 166, 232
BstV2I GAAGAC 3 cut(s) 261, 480, 498
BtsCI GGATG 3 cut(s) 367, 640, 713
BtsIMutI CAGTG 3 cut(s) 495, 573, 648
Cac8I GCNNGC 1 cut(s) 610
CciI TCATGA 1 cut(s) 457
Cfr13I GGNCC 2 cut(s) 659, 688
Csp6I GTAC 1 cut(s) 697
CviAII CATG 4 cut(s) 216, 422, 449, 458
CviQI GTAC 1 cut(s) 697
DdeI CTNAG 4 cut(s) 78, 132, 498, 848
DpnI GATC 5 cut(s) 189, 232, 297, 420, 456
DpnII GATC 5 cut(s) 187, 230, 295, 418, 454
DriI GACNNNNNGTC 1 cut(s) 817
Eam1104I CTCTTC 1 cut(s) 621
Eam1105I GACNNNNNGTC 1 cut(s) 817
EarI CTCTTC 1 cut(s) 621
Eco47I GGWCC 2 cut(s) 659, 688
Eco57I CTGAAG 1 cut(s) 956
EcoRI GAATTC 1 cut(s) 96
EcoRII CCWGG 4 cut(s) 63, 91, 855, 871
FaeI CATG 4 cut(s) 219, 425, 452, 461
FaiI YATR 9 cut(s) 126, 186, 217, 287, 359, 423, 450, 459, 617
FalI AAGNNNNNCTT 4 cut(s) 879, 911, 929, 961
FatI CATG 4 cut(s) 215, 421, 448, 457
FbaI TGATCA 1 cut(s) 187
Fnu4HI GCNGC 3 cut(s) 129, 155, 246
FokI GGATG 3 cut(s) 374, 647, 700
Fsp4HI GCNGC 3 cut(s) 129, 155, 246
FspBI CTAG 3 cut(s) 180, 234, 905
GluI GCNGC 3 cut(s) 129, 155, 246
GsuI CTGGAG 2 cut(s) 234, 878
HapII CCGG 1 cut(s) 107
Hin1II CATG 4 cut(s) 219, 425, 452, 461
HindIII AAGCTT 2 cut(s) 54, 943
HinfI GANTC 6 cut(s) 313, 476, 704, 767, 835, 852
HpaII CCGG 1 cut(s) 107
HphI GGTGA 1 cut(s) 702
Hpy166II GTNNAC 1 cut(s) 662
Hpy188I TCNGA 5 cut(s) 276, 481, 526, 751, 849
Hpy188III TCNNGA 9 cut(s) 8, 107, 180, 251, 303, 431, 458, 764, 822
Hpy8I GTNNAC 1 cut(s) 662
Hpy99I CGWCG 1 cut(s) 815
HpyAV CCTTC 2 cut(s) 360, 770
HpyCH4III ACNGT 5 cut(s) 162, 242, 519, 595, 652
HpyCH4V TGCA 6 cut(s) 128, 154, 365, 445, 487, 612
HpyF3I CTNAG 4 cut(s) 78, 132, 498, 848
Hsp92II CATG 4 cut(s) 219, 425, 452, 461
Kpn2I TCCGGA 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 5 cut(s) 187, 230, 295, 418, 454
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 1 cut(s) 253
Lsp1109I GCAGC 3 cut(s) 140, 166, 232
LweI GCATC 2 cut(s) 145, 352
MaeI CTAG 3 cut(s) 180, 234, 905
MaeIII GTNAC 3 cut(s) 321, 513, 578
MalI GATC 5 cut(s) 189, 232, 297, 420, 456
MbiI CCGCTC 1 cut(s) 845
MboI GATC 5 cut(s) 187, 230, 295, 418, 454
MboII GAAGA 7 cut(s) 261, 345, 464, 482, 485, 503, 638
MhlI GDGCHC 4 cut(s) 262, 391, 575, 728
MluCI AATT 1 cut(s) 96
MluI ACGCGT 1 cut(s) 510
MlyI GAGTC 3 cut(s) 307, 470, 698
MnlI CCTC 7 cut(s) 15, 60, 247, 348, 365, 493, 935
MroI TCCGGA 1 cut(s) 106
MroXI GAANNNNTTC 1 cut(s) 759
MseI TTAA 3 cut(s) 44, 669, 947
MslI CAYNNNNRTG 1 cut(s) 462
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 4 cut(s) 65, 93, 857, 873
MvaI CCWGG 4 cut(s) 65, 93, 857, 873
MvnI CGCG 1 cut(s) 512
NdeII GATC 5 cut(s) 187, 230, 295, 418, 454
NlaIII CATG 4 cut(s) 219, 425, 452, 461
NlaIV GGNNCC 2 cut(s) 388, 727
NmuCI GTSAC 1 cut(s) 578
PagI TCATGA 1 cut(s) 457
PciSI GCTCTTC 1 cut(s) 621
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 3 cut(s) 767, 835, 852
PfoI TCCNGGA 3 cut(s) 63, 91, 855
PkrI GCNGC 3 cut(s) 130, 156, 247
PleI GAGTC 3 cut(s) 307, 470, 698
PpsI GAGTC 3 cut(s) 307, 470, 698
Psp6I CCWGG 4 cut(s) 63, 91, 855, 871
PspGI CCWGG 4 cut(s) 63, 91, 855, 871
PspN4I GGNNCC 2 cut(s) 388, 727
PspPI GGNCC 2 cut(s) 659, 688
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 1 cut(s) 698
RsaNI GTAC 1 cut(s) 697
RseI CAYNNNNRTG 1 cut(s) 462
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 3 cut(s) 44, 669, 947
SatI GCNGC 3 cut(s) 129, 155, 246
Sau3AI GATC 5 cut(s) 187, 230, 295, 418, 454
Sau96I GGNCC 2 cut(s) 659, 688
SchI GAGTC 3 cut(s) 307, 470, 698
ScrFI CCNGG 4 cut(s) 65, 93, 857, 873
SduI GDGCHC 4 cut(s) 262, 391, 575, 728
SetI ASST 7 cut(s) 58, 133, 633, 762, 895, 927, 947
SfaNI GCATC 2 cut(s) 145, 352
SinI GGWCC 2 cut(s) 659, 688
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 3 cut(s) 51, 263, 926
SmoI CTYRAG 3 cut(s) 51, 263, 926
Sse9I AATT 1 cut(s) 96
SsiI CCGC 2 cut(s) 343, 843
SspMI CTAG 3 cut(s) 180, 234, 905
StyD4I CCNGG 4 cut(s) 63, 91, 855, 871
TaaI ACNGT 5 cut(s) 162, 242, 519, 595, 652
TaqI TCGA 1 cut(s) 294
TasI AATT 1 cut(s) 96
TfiI GAWTC 3 cut(s) 767, 835, 852
Tru1I TTAA 3 cut(s) 44, 669, 947
Tru9I TTAA 3 cut(s) 44, 669, 947
TscAI CASTG 3 cut(s) 502, 580, 655
TseFI GTSAC 1 cut(s) 578
TseI GCWGC 3 cut(s) 128, 154, 245
Tsp45I GTSAC 1 cut(s) 578
TspDTI ATGAA 6 cut(s) 204, 465, 474, 576, 651, 768
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 3 cut(s) 502, 580, 655
VpaK11BI GGWCC 2 cut(s) 659, 688
XapI RAATTY 1 cut(s) 96
XbaI TCTAGA 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 3 cut(s) 180, 234, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.