Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0020850

Physical Location & Seq

Genomic Coordinates Forward (+)

5540719 .. 5542605

Gene Structure 1887 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1047600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1026 bp

ATGTTGCTGGAGCACGTGCAACATGAATGTTTTCCATCATCTACCAACGAGGGAACTTGGCCAGTATCTGGACATATGGCATTGCCAGCGTCTACAGTTTCTGGTATTCGGAGTCTGAATGCTAACGAAGCAAATGAAGACCGTGGGTTGCTTCAATACTTACCGCCACATTTGCAGAACTTACTTCCTCCTCCAAACTCGTATCTTCATGAAAAGCAGTCACCATTTTCTTTTGAATTTGGTGGTGGGATGGCTGTACCAAATGCAAATCCAGGTTCTTCTCAGAAGGGGTTCTTTATTTTCGACCAGTCTGGGAATGAAACAAGGCTAATATACAATTCTGTTTGCCCTCCTAGCCAGTATCCACCTATGGCCAGCGCGAAGCTTGGGTGTGGTTATGATTCACATAAACTGGAACAAACAACCAGCATGGACCAAATTGGTCCAACCAAGTGCTTCTTATTTGAAGAGACCGGTGAGAATCATATAATTGAAGAGAGTGAGATGCATGAAGACACAGAAGAAATCAATGCATTGCTCTACTCTGATGATGATGATGATGATGATGATTGCGGGGAGGATGATGAAGTAAAAAGCACGGGTCACTCTCCATTCTGCCGTCAAGAGAATTATGGAAAGAACGAAGATGTTGAGGAAGTAGCAGAGAAGATGTTTAGTTCTGAATCTGATCCTCCAAACAAAAGACACAAATTGCTTGACGGTGGCTACGTGCAATTATCACCAGTGAAGACTGTCATTAATTCGTTACAACTCGATAAGTCTCATGAGTACGGGAACAATACGGAGTCAAGCAACACTCTTGGCCAGAGCCAAGGAGAGGAAGCAAGTTGGAGTAAGGGTAAGATGAAGTCGAAAACAGACAAGATTTGCGAGACAGTTCGAGTTCTTGAGAGCTTAATTCCTGGTGCTAAGGGCAAGGACCCGGTTTTGATCATCGACGAAGCTATAGACTACTTGAAGTCTATGAAGCTTAAAGCCGAAACTCTGGGAGTGAGTTTCCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009653 GO:0009826 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032502 GO:0032989 GO:0040007 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0060560 GO:0065007 GO:0071840 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

342

Amino Acids

37.7

Weight (kDa)

4.66

Isoelectric Point (pI)

56.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH_SAC51	PF23173	289 - 337	2.6e-14	Transcription factor SAC51 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0020850)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09460 AT5G64340
malus_domestica	MD04G1047600.v1.1
prunus_persica	Prupe.8G067600_v2.0.a1
pyrus_communis	pycom04g04160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	68
AccI	GTMKAC	1 cut(s)	92
AccII	CGCG	1 cut(s)	380
AciI	CCGC	2 cut(s)	164, 573
AclWI	GGATC	1 cut(s)	683
AcoI	YGGCCR	3 cut(s)	59, 372, 823
AcsI	RAATTY	1 cut(s)	236
AcvI	CACGTG	1 cut(s)	16
AfaI	GTAC	2 cut(s)	258, 791
AfiI	CCNNNNNNNGG	2 cut(s)	68, 838
AgeI	ACCGGT	1 cut(s)	473
AgsI	TTSAA	5 cut(s)	155, 236, 467, 494, 979
AjnI	CCWGG	2 cut(s)	271, 922
AluBI	AGCT	4 cut(s)	385, 915, 965, 991
AluI	AGCT	4 cut(s)	385, 915, 965, 991
Alw21I	GWGCWC	1 cut(s)	15
Alw26I	GTCTC	3 cut(s)	464, 786, 887
AlwI	GGATC	1 cut(s)	683
AlwNI	CAGNNNCTG	2 cut(s)	68, 101
AoxI	GGCC	3 cut(s)	59, 372, 823
ApoI	RAATTY	1 cut(s)	236
AseI	ATTAAT	1 cut(s)	759
AsiGI	ACCGGT	1 cut(s)	473
Asp700I	GAANNNNTTC	2 cut(s)	30, 290
AspLEI	GCGC	1 cut(s)	380
AspS9I	GGNCC	3 cut(s)	433, 443, 940
AsuC2I	CCSGG	1 cut(s)	944
AsuHPI	GGTGA	3 cut(s)	213, 488, 732
AvaII	GGWCC	3 cut(s)	433, 443, 940
BalI	TGGCCA	3 cut(s)	61, 374, 825
BbrPI	CACGTG	1 cut(s)	16
BbsI	GAAGAC	3 cut(s)	144, 519, 755
Bbv12I	GWGCWC	1 cut(s)	15
BccI	CCATC	2 cut(s)	43, 244
BceAI	ACGGC	1 cut(s)	603
BciT130I	CCWGG	2 cut(s)	273, 924
BciVI	GTATCC	1 cut(s)	372
BclI	TGATCA	1 cut(s)	951
BcnI	CCSGG	1 cut(s)	944
BcoDI	GTCTC	3 cut(s)	464, 786, 887
BfaI	CTAG	1 cut(s)	354
BfmI	CTRYAG	2 cut(s)	93, 966
BfuI	GTATCC	1 cut(s)	372
Bme1390I	CCNGG	3 cut(s)	273, 924, 944
Bme18I	GGWCC	3 cut(s)	433, 443, 940
BmgT120I	GGNCC	3 cut(s)	433, 443, 940
BmiI	GGNNCC	1 cut(s)	942
BmrFI	CCNGG	3 cut(s)	273, 924, 944
BmsI	GCATC	1 cut(s)	495
BpiI	GAAGAC	3 cut(s)	144, 519, 755
BpmI	CTGGAG	1 cut(s)	29
Bpu10I	CCTNAGC	1 cut(s)	930
BpuEI	CTTGAG	1 cut(s)	929
BpuMI	CCSGG	1 cut(s)	944
BsaAI	YACGTR	2 cut(s)	16, 730
BsaI	GGTCTC	1 cut(s)	464
BsaJI	CCNNGG	2 cut(s)	142, 832
BsaWI	WCCGGW	1 cut(s)	473
BsaXI	ACNNNNNCTCC	2 cut(s)	844, 874
Bsc4I	CCNNNNNNNGG	2 cut(s)	68, 838
Bse118I	RCCGGY	1 cut(s)	473
Bse1I	ACTGG	5 cut(s)	62, 307, 358, 417, 743
Bse3DI	GCAATG	2 cut(s)	80, 533
BseBI	CCWGG	2 cut(s)	273, 924
BseDI	CCNNGG	2 cut(s)	142, 832
BseGI	GGATG	2 cut(s)	255, 586
BseLI	CCNNNNNNNGG	2 cut(s)	68, 838
BseMI	GCAATG	2 cut(s)	80, 533
BseMII	CTCAG	1 cut(s)	296
BseNI	ACTGG	5 cut(s)	62, 307, 358, 417, 743
BseRI	GAGGAG	1 cut(s)	180
Bsh1236I	CGCG	1 cut(s)	380
BshFI	GGCC	3 cut(s)	61, 374, 825
BshTI	ACCGGT	1 cut(s)	473
BsiHKAI	GWGCWC	1 cut(s)	15
BsiSI	CCGG	2 cut(s)	474, 944
BslI	CCNNNNNNNGG	2 cut(s)	68, 838
BsmAI	GTCTC	3 cut(s)	464, 786, 887
BsmI	GAATGC	1 cut(s)	124
BsnI	GGCC	3 cut(s)	61, 374, 825
Bso31I	GGTCTC	1 cut(s)	464
Bsp1286I	GDGCHC	1 cut(s)	15
Bsp143I	GATC	2 cut(s)	688, 951
BspACI	CCGC	2 cut(s)	164, 573
BspANI	GGCC	3 cut(s)	61, 374, 825
BspCNI	CTCAG	1 cut(s)	295
BspFNI	CGCG	1 cut(s)	380
BspHI	TCATGA	2 cut(s)	208, 784
BspLI	GGNNCC	1 cut(s)	942
BspPI	GGATC	1 cut(s)	683
BspTNI	GGTCTC	1 cut(s)	464
BsrDI	GCAATG	2 cut(s)	80, 533
BsrFI	RCCGGY	1 cut(s)	473
BsrI	ACTGG	5 cut(s)	62, 307, 358, 417, 743
BssAI	RCCGGY	1 cut(s)	473
BssECI	CCNNGG	2 cut(s)	142, 832
BssMI	GATC	2 cut(s)	688, 951
BssT1I	CCWWGG	1 cut(s)	832
Bst2UI	CCWGG	2 cut(s)	273, 924
Bst4CI	ACNGT	5 cut(s)	97, 143, 722, 754, 898
Bst6I	CTCTTC	2 cut(s)	462, 489
BstBAI	YACGTR	2 cut(s)	16, 730
BstC8I	GCNNGC	2 cut(s)	87, 376
BstDEI	CTNAG	2 cut(s)	282, 930
BstDSI	CCRYGG	1 cut(s)	142
BstF5I	GGATG	2 cut(s)	255, 586
BstFNI	CGCG	1 cut(s)	380
BstHHI	GCGC	1 cut(s)	380
BstKTI	GATC	2 cut(s)	691, 954
BstMAI	GTCTC	3 cut(s)	464, 786, 887
BstMBI	GATC	2 cut(s)	688, 951
BstMWI	GCNNNNNNNGC	4 cut(s)	86, 128, 172, 354
BstNI	CCWGG	2 cut(s)	273, 924
BstSCI	CCNGG	3 cut(s)	271, 922, 942
BstSFI	CTRYAG	2 cut(s)	93, 966
BstUI	CGCG	1 cut(s)	380
BstV2I	GAAGAC	3 cut(s)	144, 519, 755
BsuI	GTATCC	1 cut(s)	372
BsuRI	GGCC	3 cut(s)	61, 374, 825
BtgI	CCRYGG	1 cut(s)	142
BtsCI	GGATG	2 cut(s)	255, 586
BtsIMutI	CAGTG	1 cut(s)	750
Cac8I	GCNNGC	2 cut(s)	87, 376
CaiI	CAGNNNCTG	2 cut(s)	68, 101
CciI	TCATGA	2 cut(s)	208, 784
CfoI	GCGC	1 cut(s)	380
Cfr10I	RCCGGY	1 cut(s)	473
Cfr13I	GGNCC	3 cut(s)	433, 443, 940
CseI	GACGC	1 cut(s)	78
Csp6I	GTAC	2 cut(s)	257, 790
CspAI	ACCGGT	1 cut(s)	473
CviAII	CATG	5 cut(s)	23, 209, 430, 509, 785
CviQI	GTAC	2 cut(s)	257, 790
DdeI	CTNAG	2 cut(s)	282, 930
DpnI	GATC	2 cut(s)	690, 953
DpnII	GATC	2 cut(s)	688, 951
EaeI	YGGCCR	3 cut(s)	59, 372, 823
Eam1104I	CTCTTC	2 cut(s)	462, 489
EarI	CTCTTC	2 cut(s)	462, 489
Eco130I	CCWWGG	1 cut(s)	832
Eco31I	GGTCTC	1 cut(s)	464
Eco47I	GGWCC	3 cut(s)	433, 443, 940
Eco72I	CACGTG	1 cut(s)	16
EcoO109I	RGGNCCY	1 cut(s)	940
EcoRII	CCWGG	2 cut(s)	271, 922
EcoT14I	CCWWGG	1 cut(s)	832
EcoT22I	ATGCAT	2 cut(s)	510, 535
ErhI	CCWWGG	1 cut(s)	832
FaeI	CATG	5 cut(s)	26, 212, 433, 512, 788
FalI	AAGNNNNNCTT	4 cut(s)	278, 310, 443, 475
FatI	CATG	5 cut(s)	22, 208, 429, 508, 784
FauI	CCCGC	1 cut(s)	566
FauNDI	CATATG	1 cut(s)	75
FbaI	TGATCA	1 cut(s)	951
FblI	GTMKAC	1 cut(s)	92
FokI	GGATG	2 cut(s)	262, 593
FspBI	CTAG	1 cut(s)	354
GlaI	GCGC	1 cut(s)	379
GsuI	CTGGAG	1 cut(s)	29
HaeIII	GGCC	3 cut(s)	61, 374, 825
HapII	CCGG	2 cut(s)	474, 944
HgaI	GACGC	1 cut(s)	78
HhaI	GCGC	1 cut(s)	380
Hin1II	CATG	5 cut(s)	26, 212, 433, 512, 788
Hin6I	GCGC	1 cut(s)	378
HinP1I	GCGC	1 cut(s)	378
HindIII	AAGCTT	2 cut(s)	383, 989
HinfI	GANTC	5 cut(s)	112, 401, 481, 683, 806
HpaII	CCGG	2 cut(s)	474, 944
HphI	GGTGA	3 cut(s)	213, 488, 732
Hpy166II	GTNNAC	1 cut(s)	93
Hpy188I	TCNGA	6 cut(s)	111, 117, 285, 547, 682, 688
Hpy188III	TCNNGA	5 cut(s)	69, 209, 623, 785, 908
Hpy8I	GTNNAC	1 cut(s)	93
Hpy99I	CGWCG	1 cut(s)	962
HpyAV	CCTTC	1 cut(s)	280
HpyCH4III	ACNGT	5 cut(s)	97, 143, 722, 754, 898
HpyCH4IV	ACGT	2 cut(s)	15, 729
HpyCH4V	TGCA	6 cut(s)	19, 175, 266, 508, 533, 733
HpyF10VI	GCNNNNNNNGC	4 cut(s)	86, 128, 172, 354
HpyF3I	CTNAG	2 cut(s)	282, 930
HpySE526I	ACGT	2 cut(s)	15, 729
Hsp92II	CATG	5 cut(s)	26, 212, 433, 512, 788
HspAI	GCGC	1 cut(s)	378
Ksp22I	TGATCA	1 cut(s)	951
Kzo9I	GATC	2 cut(s)	688, 951
LmnI	GCTCC	1 cut(s)	10
LweI	GCATC	1 cut(s)	495
MaeI	CTAG	1 cut(s)	354
MaeII	ACGT	2 cut(s)	15, 729
MaeIII	GTNAC	3 cut(s)	219, 602, 765
MalI	GATC	2 cut(s)	690, 953
MboI	GATC	2 cut(s)	688, 951
MhlI	GDGCHC	1 cut(s)	15
MlsI	TGGCCA	3 cut(s)	61, 374, 825
MluCI	AATT	9 cut(s)	236, 337, 438, 489, 628, 710, 734, 760, 918
MluNI	TGGCCA	3 cut(s)	61, 374, 825
MlyI	GAGTC	2 cut(s)	121, 815
MmeI	TCCRAC	2 cut(s)	470, 830
MnlI	CCTC	8 cut(s)	43, 198, 201, 360, 571, 646, 702, 832
Mox20I	TGGCCA	3 cut(s)	61, 374, 825
Mph1103I	ATGCAT	2 cut(s)	510, 535
MroXI	GAANNNNTTC	2 cut(s)	30, 290
MscI	TGGCCA	3 cut(s)	61, 374, 825
MseI	TTAA	3 cut(s)	759, 917, 993
Msp20I	TGGCCA	3 cut(s)	61, 374, 825
MspI	CCGG	2 cut(s)	474, 944
MspR9I	CCNGG	3 cut(s)	273, 924, 944
Mva1269I	GAATGC	1 cut(s)	124
MvaI	CCWGG	2 cut(s)	273, 924
MvnI	CGCG	1 cut(s)	380
MwoI	GCNNNNNNNGC	4 cut(s)	86, 128, 172, 354
NciI	CCSGG	1 cut(s)	944
NdeI	CATATG	1 cut(s)	75
NdeII	GATC	2 cut(s)	688, 951
NlaIII	CATG	5 cut(s)	26, 212, 433, 512, 788
NlaIV	GGNNCC	1 cut(s)	942
NmuCI	GTSAC	2 cut(s)	219, 602
NsiI	ATGCAT	2 cut(s)	510, 535
PagI	TCATGA	2 cut(s)	208, 784
PcsI	WCGNNNNNNNCGW	1 cut(s)	726
PctI	GAATGC	1 cut(s)	124
PdmI	GAANNNNTTC	2 cut(s)	30, 290
PfeI	GAWTC	3 cut(s)	401, 481, 683
PflMI	CCANNNNNTGG	1 cut(s)	68
PinAI	ACCGGT	1 cut(s)	473
PleI	GAGTC	2 cut(s)	120, 814
PmaCI	CACGTG	1 cut(s)	16
PmlI	CACGTG	1 cut(s)	16
PpsI	GAGTC	2 cut(s)	120, 814
Ppu21I	YACGTR	2 cut(s)	16, 730
PpuMI	RGGWCCY	1 cut(s)	940
PshBI	ATTAAT	1 cut(s)	759
Psp5II	RGGWCCY	1 cut(s)	940
Psp6I	CCWGG	2 cut(s)	271, 922
PspCI	CACGTG	1 cut(s)	16
PspGI	CCWGG	2 cut(s)	271, 922
PspN4I	GGNNCC	1 cut(s)	942
PspPI	GGNCC	3 cut(s)	433, 443, 940
PspPPI	RGGWCCY	1 cut(s)	940
PstNI	CAGNNNCTG	2 cut(s)	68, 101
RsaI	GTAC	2 cut(s)	258, 791
RsaNI	GTAC	2 cut(s)	257, 790
SaqAI	TTAA	3 cut(s)	759, 917, 993
Sau3AI	GATC	2 cut(s)	688, 951
Sau96I	GGNCC	3 cut(s)	433, 443, 940
SchI	GAGTC	2 cut(s)	121, 815
ScrFI	CCNGG	3 cut(s)	273, 924, 944
SduI	GDGCHC	1 cut(s)	15
SetI	ASST	8 cut(s)	18, 277, 370, 387, 732, 917, 967, 993
SfaNI	GCATC	1 cut(s)	495
SfcI	CTRYAG	2 cut(s)	93, 966
SinI	GGWCC	3 cut(s)	433, 443, 940
SmlI	CTYRAG	1 cut(s)	908
SmoI	CTYRAG	1 cut(s)	908
Sse9I	AATT	9 cut(s)	236, 337, 438, 489, 628, 710, 734, 760, 918
SsiI	CCGC	2 cut(s)	164, 573
SspMI	CTAG	1 cut(s)	354
StyD4I	CCNGG	3 cut(s)	271, 922, 942
StyI	CCWWGG	1 cut(s)	832
TaaI	ACNGT	5 cut(s)	97, 143, 722, 754, 898
TaiI	ACGT	2 cut(s)	18, 732
TaqI	TCGA	5 cut(s)	303, 774, 872, 901, 957
TasI	AATT	9 cut(s)	236, 337, 438, 489, 628, 710, 734, 760, 918
TfiI	GAWTC	3 cut(s)	401, 481, 683
Tru1I	TTAA	3 cut(s)	759, 917, 993
Tru9I	TTAA	3 cut(s)	759, 917, 993
TscAI	CASTG	1 cut(s)	750
TseFI	GTSAC	2 cut(s)	219, 602
Tsp45I	GTSAC	2 cut(s)	219, 602
TspDTI	ATGAA	9 cut(s)	39, 150, 197, 225, 333, 525, 600, 881, 1001
TspGWI	ACGGA	1 cut(s)	818
TspRI	CASTG	1 cut(s)	750
Van91I	CCANNNNNTGG	1 cut(s)	68
VpaK11BI	GGWCC	3 cut(s)	433, 443, 940
VspI	ATTAAT	1 cut(s)	759
XapI	RAATTY	1 cut(s)	236
XmiI	GTMKAC	1 cut(s)	92
XmnI	GAANNNNTTC	2 cut(s)	30, 290
XspI	CTAG	1 cut(s)	354
Zsp2I	ATGCAT	2 cut(s)	510, 535

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.