AT5G64340

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0020850
Physical Location & Seq
Reverse (-)
25730335 .. 25733313
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64340.1

Sequence Viewer

Length: 1047 bp
ATGCCTCTGGATAAGAGGCAACGGGATTTGCCTCTGGGCTTAAGTCCTCAAGCTTGCTTCAAGGATATAGTAGGTCGGTCTGTCCTTCCTAGAATTCCTCTCCCTGAGCTTGGGAAACTATATGCAGCTAAGCTTCAGGCTCGCTGTTTGCAGCCACCACCATTCCAGTCTTTGCTGTGCAGTCATGATAAGGAGTCTTATGGAAAAAGATTCTCACGGTCTGACATGCGGTCTTGGTGCGCTGCTGCTACTACTACTACTACTCCACTTGGAGCATTAGAGTCTTCTCAGAAAAGACTTTTGATATTCGATCAGTCAGGAGACCAGACTCGTCTATTACAATGTCCATTTCCTCTACGGTTTCCATCTCATGCGGCTGCAGAACCAGTGAAACTCTCTGAGTTACAAGGTATAGAGAAAGCTTTCAAAGAAGATGGTGAAGAGTTTCACAAGAGTGATGGAACAGAGTCAGAAATGCATGAAGACACTGAGGAGATCAATGCATTGCTATATTCAGATGATGATTATGATGATGATTGCGAGAGTGATGATGAAGTAATGAGCACTGGTCACTCTCCTTATCCAAATGAAGGAGTTTGCAACAAAAGGGAATTAGAAGAAATCGATGGTCCTTGTAAAAGGCAGAAACTACTGGATAAGGTCAACAACATCAGCGACTTATCATCACTTGTGGGCACTGAGAGCTCCACACAACTCAATGGATCTTCCTTTCTTAAGGACAAAAAGCTCCCTGAATCAAAAACCATATCGACCAAAGAGGACACTGGTTCTGGTCTGAGCAACGAGCAGTCGAAGAAAGACAAGATCCGCACAGCTCTGAAAATACTCGAGAGCGTAGTCCCTGGTGCAAAAGGAAACGAAGCGCTCTTACTTCTGGACGAAGCAATTGATTACCTAAAGTTGCTGAAACGAGACTTAATCTCCACAGAGGTTAAGAACCAAAGCTCCACCACTCACAAGTCACCAATCTTGTTGCTTAAAGAGACAACATGGGGAACAAGAAATCTGCAGACAGATAAGGCGTGA

Protein Analysis

348

Amino Acids

38.7

Weight (kDa)

5.3

Isoelectric Point (pI)

62.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_SAC51 PF23173 260 - 322 8.6e-36 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09460 AT5G64340
malus_domestica MD04G1047600.v1.1
prunus_persica Prupe.8G067600_v2.0.a1
pyrus_communis pycom04g04160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 229, 374, 829
AclWI GGATC 2 cut(s) 730, 820
AcsI RAATTY 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 119
AfeI AGCGCT 1 cut(s) 885
AfiI CCNNNNNNNGG 2 cut(s) 110, 590
AflII CTTAAG 2 cut(s) 40, 734
AgsI TTSAA 2 cut(s) 61, 427
AhdI GACNNNNNGTC 1 cut(s) 229
AjnI CCWGG 1 cut(s) 862
AleI CACNNNNGTG 1 cut(s) 453
AloI GAACNNNNNNTCC 2 cut(s) 950, 982
AluBI AGCT 9 cut(s) 53, 109, 128, 133, 422, 705, 748, 836, 966
AluI AGCT 9 cut(s) 53, 109, 128, 133, 422, 705, 748, 836, 966
Alw21I GWGCWC 2 cut(s) 566, 707
Alw26I GTCTC 3 cut(s) 315, 927, 998
AlwI GGATC 2 cut(s) 730, 820
Ama87I CYCGRG 1 cut(s) 848
Aor51HI AGCGCT 1 cut(s) 885
ApeKI GCWGC 5 cut(s) 125, 151, 242, 245, 377
ApoI RAATTY 1 cut(s) 93
Asp700I GAANNNNTTC 2 cut(s) 422, 444
AspLEI GCGC 2 cut(s) 242, 886
AspS9I GGNCC 1 cut(s) 629
AsuHPI GGTGA 2 cut(s) 449, 975
AvaI CYCGRG 1 cut(s) 848
AvaII GGWCC 1 cut(s) 629
BaeGI GKGCMC 1 cut(s) 698
BanII GRGCYC 1 cut(s) 707
BarI GAAGNNNNNNTAC 2 cut(s) 873, 905
BbsI GAAGAC 2 cut(s) 276, 489
Bbv12I GWGCWC 2 cut(s) 566, 707
BbvI GCAGC 5 cut(s) 137, 163, 229, 232, 364
BccI CCATC 4 cut(s) 373, 428, 452, 620
BciT130I CCWGG 1 cut(s) 864
BcoDI GTCTC 3 cut(s) 315, 927, 998
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 2 cut(s) 378, 1028
BfoI RGCGCY 1 cut(s) 887
BfrI CTTAAG 2 cut(s) 40, 734
BisI GCNGC 6 cut(s) 126, 152, 243, 246, 375, 378
BlpI GCTNAGC 1 cut(s) 129
BlsI GCNGC 6 cut(s) 127, 153, 244, 247, 376, 379
Bme1390I CCNGG 1 cut(s) 864
Bme18I GGWCC 1 cut(s) 629
BmeRI GACNNNNNGTC 1 cut(s) 229
BmeT110I CYCGRG 1 cut(s) 848
BmgT120I GGNCC 1 cut(s) 629
BmrFI CCNGG 1 cut(s) 864
BpiI GAAGAC 2 cut(s) 276, 489
Bpu10I CCTNAGC 1 cut(s) 105
Bpu1102I GCTNAGC 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 33
Bsa29I ATCGAT 1 cut(s) 624
BsaI GGTCTC 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 862
BsaXI ACNNNNNCTCC 6 cut(s) 247, 277, 926, 950, 956, 980
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 590
Bse1I ACTGG 5 cut(s) 166, 386, 571, 657, 790
Bse3DI GCAATG 1 cut(s) 503
BseBI CCWGG 1 cut(s) 864
BseCI ATCGAT 1 cut(s) 624
BseDI CCNNGG 1 cut(s) 862
BseLI CCNNNNNNNGG 2 cut(s) 110, 590
BseMI GCAATG 1 cut(s) 503
BseMII CTCAG 6 cut(s) 96, 302, 390, 480, 690, 788
BseNI ACTGG 5 cut(s) 166, 386, 571, 657, 790
BseRI GAGGAG 1 cut(s) 506
BseSI GKGCMC 1 cut(s) 698
BseXI GCAGC 5 cut(s) 137, 163, 229, 232, 364
BsgI GTGCAG 1 cut(s) 199
BshVI ATCGAT 1 cut(s) 624
BsiHKAI GWGCWC 2 cut(s) 566, 707
BsiHKCI CYCGRG 1 cut(s) 848
BslFI GGGAC 1 cut(s) 845
BslI CCNNNNNNNGG 2 cut(s) 110, 590
BsmAI GTCTC 3 cut(s) 315, 927, 998
BsmFI GGGAC 1 cut(s) 845
Bso31I GGTCTC 1 cut(s) 315
BsoBI CYCGRG 1 cut(s) 848
Bsp1286I GDGCHC 3 cut(s) 566, 698, 707
Bsp143I GATC 4 cut(s) 310, 495, 722, 825
Bsp1720I GCTNAGC 1 cut(s) 129
BspACI CCGC 3 cut(s) 229, 374, 829
BspCNI CTCAG 6 cut(s) 97, 301, 391, 481, 691, 789
BspDI ATCGAT 1 cut(s) 624
BspHI TCATGA 1 cut(s) 184
BspMAI CTGCAG 2 cut(s) 382, 1032
BspPI GGATC 2 cut(s) 730, 820
BspTI CTTAAG 2 cut(s) 40, 734
BspTNI GGTCTC 1 cut(s) 315
BsrDI GCAATG 1 cut(s) 503
BsrI ACTGG 5 cut(s) 166, 386, 571, 657, 790
BssECI CCNNGG 1 cut(s) 862
BssMI GATC 4 cut(s) 310, 495, 722, 825
Bst2UI CCWGG 1 cut(s) 864
Bst4CI ACNGT 2 cut(s) 219, 360
Bst6I CTCTTC 1 cut(s) 435
BstAFI CTTAAG 2 cut(s) 40, 734
BstC8I GCNNGC 2 cut(s) 55, 142
BstDEI CTNAG 7 cut(s) 105, 129, 288, 399, 489, 699, 797
BstH2I RGCGCY 1 cut(s) 887
BstHHI GCGC 2 cut(s) 242, 886
BstKTI GATC 4 cut(s) 313, 498, 725, 828
BstMAI GTCTC 3 cut(s) 315, 927, 998
BstMBI GATC 4 cut(s) 310, 495, 722, 825
BstMWI GCNNNNNNNGC 1 cut(s) 702
BstNI CCWGG 1 cut(s) 864
BstNSI RCATGY 1 cut(s) 229
BstSCI CCNGG 1 cut(s) 862
BstSFI CTRYAG 2 cut(s) 378, 1028
BstSLI GKGCMC 1 cut(s) 698
BstV1I GCAGC 5 cut(s) 137, 163, 229, 232, 364
BstV2I GAAGAC 2 cut(s) 276, 489
BstX2I RGATCY 2 cut(s) 722, 825
BstYI RGATCY 2 cut(s) 722, 825
Bsu15I ATCGAT 1 cut(s) 624
BsuTUI ATCGAT 1 cut(s) 624
BtsIMutI CAGTG 5 cut(s) 393, 486, 564, 696, 783
Cac8I GCNNGC 2 cut(s) 55, 142
CciI TCATGA 1 cut(s) 184
CfoI GCGC 2 cut(s) 242, 886
Cfr13I GGNCC 1 cut(s) 629
ClaI ATCGAT 1 cut(s) 624
CviAII CATG 5 cut(s) 185, 226, 371, 479, 1011
DdeI CTNAG 7 cut(s) 105, 129, 288, 399, 489, 699, 797
DpnI GATC 4 cut(s) 312, 497, 724, 827
DpnII GATC 4 cut(s) 310, 495, 722, 825
DriI GACNNNNNGTC 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 435
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 1 cut(s) 435
Ecl136II GAGCTC 1 cut(s) 705
Eco24I GRGCYC 1 cut(s) 707
Eco31I GGTCTC 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 629
Eco47III AGCGCT 1 cut(s) 885
Eco53kI GAGCTC 1 cut(s) 705
Eco57I CTGAAG 1 cut(s) 119
Eco88I CYCGRG 1 cut(s) 848
EcoICRI GAGCTC 1 cut(s) 705
EcoRI GAATTC 1 cut(s) 93
EcoRII CCWGG 1 cut(s) 862
EcoT22I ATGCAT 2 cut(s) 480, 505
EcoT38I GRGCYC 1 cut(s) 707
FaeI CATG 5 cut(s) 188, 229, 374, 482, 1014
FaqI GGGAC 1 cut(s) 845
FatI CATG 5 cut(s) 184, 225, 370, 478, 1010
Fnu4HI GCNGC 6 cut(s) 126, 152, 243, 246, 375, 378
FriOI GRGCYC 1 cut(s) 707
Fsp4HI GCNGC 6 cut(s) 126, 152, 243, 246, 375, 378
FspBI CTAG 1 cut(s) 90
GlaI GCGC 2 cut(s) 241, 885
GluI GCNGC 6 cut(s) 126, 152, 243, 246, 375, 378
HaeII RGCGCY 1 cut(s) 887
HhaI GCGC 2 cut(s) 242, 886
Hin1II CATG 5 cut(s) 188, 229, 374, 482, 1014
Hin6I GCGC 2 cut(s) 240, 884
HinP1I GCGC 2 cut(s) 240, 884
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 3 cut(s) 51, 131, 420
HinfI GANTC 6 cut(s) 194, 210, 281, 328, 467, 755
HphI GGTGA 2 cut(s) 449, 975
Hpy166II GTNNAC 1 cut(s) 664
Hpy188I TCNGA 7 cut(s) 223, 291, 400, 472, 517, 798, 840
Hpy188III TCNNGA 5 cut(s) 8, 185, 318, 850, 896
Hpy8I GTNNAC 1 cut(s) 664
HpyAV CCTTC 2 cut(s) 95, 584
HpyCH4III ACNGT 2 cut(s) 219, 360
HpyCH4V TGCA 9 cut(s) 125, 151, 180, 380, 478, 503, 600, 869, 1030
HpyF10VI GCNNNNNNNGC 1 cut(s) 702
HpyF3I CTNAG 7 cut(s) 105, 129, 288, 399, 489, 699, 797
Hsp92II CATG 5 cut(s) 188, 229, 374, 482, 1014
HspAI GCGC 2 cut(s) 240, 884
Kzo9I GATC 4 cut(s) 310, 495, 722, 825
LmnI GCTCC 4 cut(s) 272, 710, 753, 971
Lsp1109I GCAGC 5 cut(s) 137, 163, 229, 232, 364
MaeI CTAG 1 cut(s) 90
MaeIII GTNAC 3 cut(s) 402, 569, 981
MalI GATC 4 cut(s) 312, 497, 724, 827
MboI GATC 4 cut(s) 310, 495, 722, 825
MboII GAAGA 7 cut(s) 276, 443, 452, 494, 629, 717, 826
MfeI CAATTG 1 cut(s) 906
MflI RGATCY 2 cut(s) 722, 825
MhlI GDGCHC 3 cut(s) 566, 698, 707
MluCI AATT 3 cut(s) 93, 611, 906
MlyI GAGTC 4 cut(s) 203, 290, 322, 476
MnlI CCTC 9 cut(s) 9, 15, 42, 57, 108, 363, 484, 772, 943
Mph1103I ATGCAT 2 cut(s) 480, 505
MroXI GAANNNNTTC 2 cut(s) 422, 444
MseI TTAA 5 cut(s) 41, 735, 938, 954, 999
MslI CAYNNNNRTG 1 cut(s) 453
MspCI CTTAAG 2 cut(s) 40, 734
MspR9I CCNGG 1 cut(s) 864
MunI CAATTG 1 cut(s) 906
MvaI CCWGG 1 cut(s) 864
MwoI GCNNNNNNNGC 1 cut(s) 702
NdeII GATC 4 cut(s) 310, 495, 722, 825
NlaIII CATG 5 cut(s) 188, 229, 374, 482, 1014
NmuCI GTSAC 2 cut(s) 569, 981
NsiI ATGCAT 2 cut(s) 480, 505
NspI RCATGY 1 cut(s) 229
OliI CACNNNNGTG 1 cut(s) 453
PaeR7I CTCGAG 1 cut(s) 848
PagI TCATGA 1 cut(s) 184
PdmI GAANNNNTTC 2 cut(s) 422, 444
PfeI GAWTC 2 cut(s) 210, 755
PkrI GCNGC 6 cut(s) 127, 153, 244, 247, 376, 379
PleI GAGTC 4 cut(s) 202, 289, 322, 475
PpsI GAGTC 4 cut(s) 202, 289, 322, 475
Psp124BI GAGCTC 1 cut(s) 707
Psp6I CCWGG 1 cut(s) 862
PspGI CCWGG 1 cut(s) 862
PspPI GGNCC 1 cut(s) 629
PstI CTGCAG 2 cut(s) 382, 1032
PsuI RGATCY 2 cut(s) 722, 825
RseI CAYNNNNRTG 1 cut(s) 453
SacI GAGCTC 1 cut(s) 707
SaqAI TTAA 5 cut(s) 41, 735, 938, 954, 999
SatI GCNGC 6 cut(s) 126, 152, 243, 246, 375, 378
Sau3AI GATC 4 cut(s) 310, 495, 722, 825
Sau96I GGNCC 1 cut(s) 629
SchI GAGTC 4 cut(s) 203, 290, 322, 476
ScrFI CCNGG 1 cut(s) 864
SduI GDGCHC 3 cut(s) 566, 698, 707
SfcI CTRYAG 2 cut(s) 378, 1028
Sfr274I CTCGAG 1 cut(s) 848
SinI GGWCC 1 cut(s) 629
SlaI CTCGAG 1 cut(s) 848
SmiMI CAYNNNNRTG 1 cut(s) 453
SmlI CTYRAG 4 cut(s) 40, 48, 734, 848
SmoI CTYRAG 4 cut(s) 40, 48, 734, 848
Sse9I AATT 3 cut(s) 93, 611, 906
SsiI CCGC 3 cut(s) 229, 374, 829
SspMI CTAG 1 cut(s) 90
SstI GAGCTC 1 cut(s) 707
StyD4I CCNGG 1 cut(s) 862
TaaI ACNGT 2 cut(s) 219, 360
TaqI TCGA 5 cut(s) 309, 624, 770, 812, 849
TaqII GACCGA 1 cut(s) 66
TasI AATT 3 cut(s) 93, 611, 906
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 2 cut(s) 210, 755
Tru1I TTAA 5 cut(s) 41, 735, 938, 954, 999
Tru9I TTAA 5 cut(s) 41, 735, 938, 954, 999
TscAI CASTG 5 cut(s) 393, 493, 571, 703, 790
TseFI GTSAC 2 cut(s) 569, 981
TseI GCWGC 5 cut(s) 125, 151, 242, 245, 377
Tsp45I GTSAC 2 cut(s) 569, 981
TspDTI ATGAA 3 cut(s) 495, 567, 603
TspRI CASTG 5 cut(s) 393, 493, 571, 703, 790
Vha464I CTTAAG 2 cut(s) 40, 734
VpaK11BI GGWCC 1 cut(s) 629
XapI RAATTY 1 cut(s) 93
XceI RCATGY 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 848
XmnI GAANNNNTTC 2 cut(s) 422, 444
XspI CTAG 1 cut(s) 90
Zsp2I ATGCAT 2 cut(s) 480, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.