AT5G19380

Crt homolog 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0019133
Physical Location & Seq
Reverse (-)
6526807 .. 6530713
3907 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G19380.2

Sequence Viewer

Length: 1344 bp
ATGGCAACAACGAGCTCCGACCGGTTAATCGCCGGTTTAACGGCGTCGATCGGCTCAATCGAATCTCGTTACGCAAATCCAGCTCAATCCGTTTCTCTAATCTGTCGAAATCAAATCAATGGCGCACCGCCTATCGTCTTGCGGTCCTCTCGAAGGTCCAGGCTGTGGTTAATCGAGGCGATTCCTCCGGCAAAGTCCTGGAACGGATCTAACGATGGCGACGAAGATATCAAGAAGTCAGACACTCGTAATTACGCGATCGGTGGAACCGGTGGTCACGCGGTCGCCGGAAAAGATGACAGAACGATGGAGATAGTAATAGCAGCGGCGACCACGGCGGCGCTGGGTGTCGGGAATCGCGTACTGTATAAGCTGGCTCTTATTCCTCTCAAGCAATATCCCTTCTTCCTCGCGCAACTCTCCACATTCGGGTATGTAGCTGTATACTTTTCGATTTTGTATTTCAGATACCGAGCTGGAATTGTTACAAAAGAGATGCTATCAGTTCCAAAACTTCCATTTTTAATTGTTGGCGTCTTGGAGTCTCTTGCTCTAGCTGCTGGGATGGCGGCCGCATCAAATCTTTCTGGACCATCTACTACAGTTTTATCTCAGACATTTCTTGTCTGGCAAATTCTTTTCTCCATTATATTTCTCGGGAGGAGATATAGAATAAATCAAATATTGGGATGTACTCTTGTAGCATTTGGTGTAATCGTCAGTGTAGCAAGTGGGTCGGGTGCCGCTCATTCATTTAAAGATACAGGAATACTTTGGAGTCTTCTTATGGTGTTTTCTTTTCTGCTTCAAGGAGCAGATACAGTAATGAAGGAAGTCATCTTTCTAGATAGCAAAAAGCGATTGAAGGGTGCTTCACTTGATTTGTTTGTTGTAAACTCATATGGTTCAATTTTCCAAGTCATATGCATTGCGTTGCTTCTTCCTTTTCTTTCAAAGCTTTGGGGCATACCGTTTAACCAACTACCGAGCTATATCAGAGACGGGGGTGCTTGCTTTCTAAATATTGGTTCTAGAATAACAGGATGCGAAGGGGCTCCCCTTCTTCCTGTAATGTTTGTCATGATGAACATGGCTTATAACATCTCTCTTCTACGCCTCATCAAGATTTCATCTGCAGTTGTGTCGTCTCTGGCATCCACCGTTTCAGTGCCCATAGCGGTGTACTGTTTCACGCTTCCCCTACCATACCTTGGGGTGGCATCTACACTACCAAGGGGGTTTGTTGCAGGCACAATCATCCTTGTAGTCGGCATGCTTCTATATGCTTGGACACCATCTACTAACACTTCCGATTCAATTATCCCCTCACCCCCATCCACATAG

Protein Analysis

447

Amino Acids

47.92

Weight (kDa)

9.57

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRT-like PF08627 204 - 488 4.2e-32 CRT-like, chloroquine-resistance transporter-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12170 AT5G12170 AT5G19380 AT5G19380
malus_domestica MD03G1165200.v1.1
prunus_persica Prupe.4G263900_v2.0.a1 Prupe.4G263900_v2.0.a1
pyrus_communis pycom03g11980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1098
AccB1I GGYRCC 1 cut(s) 740
AccB7I CCANNNNNTGG 2 cut(s) 165, 1211
AccBSI CCGCTC 1 cut(s) 746
AccI GTMKAC 1 cut(s) 444
AccII CGCG 4 cut(s) 257, 281, 360, 413
AciI CCGC 9 cut(s) 128, 142, 281, 326, 338, 569, 573, 744, 1178
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 1 cut(s) 570
AcsI RAATTY 1 cut(s) 633
AcyI GRCGYC 2 cut(s) 44, 534
AfaI GTAC 3 cut(s) 363, 694, 1184
AfiI CCNNNNNNNGG 5 cut(s) 153, 165, 429, 1211, 1216
AgeI ACCGGT 2 cut(s) 21, 269
AgsI TTSAA 5 cut(s) 809, 865, 909, 954, 1317
AjnI CCWGG 2 cut(s) 158, 197
AluBI AGCT 8 cut(s) 15, 83, 373, 440, 476, 557, 958, 990
AluI AGCT 8 cut(s) 15, 83, 373, 440, 476, 557, 958, 990
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 3 cut(s) 549, 993, 1152
AlwI GGATC 1 cut(s) 214
Ama87I CYCGRG 1 cut(s) 656
AoxI GGCC 1 cut(s) 570
ApeKI GCWGC 2 cut(s) 323, 557
ApoI RAATTY 1 cut(s) 633
AsiGI ACCGGT 2 cut(s) 21, 269
AspLEI GCGC 3 cut(s) 125, 343, 415
AspS9I GGNCC 3 cut(s) 144, 156, 590
AsuHPI GGTGA 1 cut(s) 1320
AvaI CYCGRG 1 cut(s) 656
AvaII GGWCC 3 cut(s) 144, 156, 590
BaeGI GKGCMC 1 cut(s) 1173
BanI GGYRCC 1 cut(s) 740
BanII GRGCYC 2 cut(s) 17, 1057
BbsI GAAGAC 1 cut(s) 773
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 2 cut(s) 335, 544
BccI CCATC 6 cut(s) 209, 301, 559, 601, 1303, 1342
BceAI ACGGC 2 cut(s) 57, 351
BcgI CGANNNNNNTGC 4 cut(s) 717, 751, 1125, 1159
BciT130I CCWGG 2 cut(s) 160, 199
BcoDI GTCTC 3 cut(s) 549, 993, 1152
BfaI CTAG 3 cut(s) 554, 845, 1032
BfmI CTRYAG 2 cut(s) 600, 1134
BfoI RGCGCY 1 cut(s) 344
BisI GCNGC 7 cut(s) 324, 327, 339, 558, 570, 573, 744
BlsI GCNGC 7 cut(s) 325, 328, 340, 559, 571, 574, 745
Bme1390I CCNGG 2 cut(s) 160, 199
Bme18I GGWCC 3 cut(s) 144, 156, 590
BmeT110I CYCGRG 1 cut(s) 656
BmgT120I GGNCC 3 cut(s) 144, 156, 590
BmiI GGNNCC 3 cut(s) 268, 742, 1056
BmrFI CCNGG 2 cut(s) 160, 199
BmsI GCATC 5 cut(s) 486, 584, 1034, 1163, 1229
BpiI GAAGAC 1 cut(s) 773
BpuEI CTTGAG 1 cut(s) 374
BsaHI GRCGYC 2 cut(s) 44, 534
BsaJI CCNNGG 3 cut(s) 333, 1210, 1232
BsaWI WCCGGW 2 cut(s) 21, 269
Bsc4I CCNNNNNNNGG 5 cut(s) 153, 165, 429, 1211, 1216
Bse118I RCCGGY 3 cut(s) 21, 32, 269
Bse3DI GCAATG 1 cut(s) 927
BseBI CCWGG 2 cut(s) 160, 199
BseDI CCNNGG 3 cut(s) 333, 1210, 1232
BseGI GGATG 6 cut(s) 570, 695, 1049, 1154, 1257, 1334
BseLI CCNNNNNNNGG 5 cut(s) 153, 165, 429, 1211, 1216
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 1 cut(s) 626
BseRI GAGGAG 1 cut(s) 676
BseSI GKGCMC 1 cut(s) 1173
BseX3I CGGCCG 1 cut(s) 570
BseXI GCAGC 2 cut(s) 335, 544
BseYI CCCAGC 2 cut(s) 343, 560
Bsh1236I CGCG 4 cut(s) 257, 281, 360, 413
Bsh1285I CGRYCG 5 cut(s) 22, 51, 261, 285, 573
BshFI GGCC 1 cut(s) 572
BshNI GGYRCC 1 cut(s) 740
BshTI ACCGGT 2 cut(s) 21, 269
BsiEI CGRYCG 5 cut(s) 22, 51, 261, 285, 573
BsiHKAI GWGCWC 1 cut(s) 17
BsiHKCI CYCGRG 1 cut(s) 656
BsiSI CCGG 5 cut(s) 22, 33, 188, 270, 288
BslI CCNNNNNNNGG 5 cut(s) 153, 165, 429, 1211, 1216
BsmAI GTCTC 3 cut(s) 549, 993, 1152
BsmBI CGTCTC 2 cut(s) 993, 1152
BsnI GGCC 1 cut(s) 572
BsoBI CYCGRG 1 cut(s) 656
Bsp1286I GDGCHC 3 cut(s) 17, 1057, 1173
Bsp143I GATC 3 cut(s) 48, 206, 258
BspACI CCGC 9 cut(s) 128, 142, 281, 326, 338, 569, 573, 744, 1178
BspANI GGCC 1 cut(s) 572
BspCNI CTCAG 1 cut(s) 625
BspFNI CGCG 4 cut(s) 257, 281, 360, 413
BspHI TCATGA 1 cut(s) 1080
BspLI GGNNCC 3 cut(s) 268, 742, 1056
BspMAI CTGCAG 1 cut(s) 1138
BspPI GGATC 1 cut(s) 214
BspT107I GGYRCC 1 cut(s) 740
BsrBI CCGCTC 1 cut(s) 746
BsrDI GCAATG 1 cut(s) 927
BsrFI RCCGGY 3 cut(s) 21, 32, 269
BssAI RCCGGY 3 cut(s) 21, 32, 269
BssECI CCNNGG 3 cut(s) 333, 1210, 1232
BssMI GATC 3 cut(s) 48, 206, 258
BssNAI GTATAC 1 cut(s) 445
BssNI GRCGYC 2 cut(s) 44, 534
BssT1I CCWWGG 2 cut(s) 1210, 1232
Bst1107I GTATAC 1 cut(s) 445
Bst2UI CCWGG 2 cut(s) 160, 199
Bst4CI ACNGT 6 cut(s) 366, 604, 823, 972, 1162, 1187
Bst6I CTCTTC 1 cut(s) 1113
BstACI GRCGYC 2 cut(s) 44, 534
BstC8I GCNNGC 4 cut(s) 375, 1012, 1249, 1274
BstDEI CTNAG 1 cut(s) 612
BstDSI CCRYGG 1 cut(s) 333
BstENI CCTNNNNNAGG 1 cut(s) 151
BstF5I GGATG 6 cut(s) 570, 695, 1049, 1154, 1257, 1334
BstFNI CGCG 4 cut(s) 257, 281, 360, 413
BstH2I RGCGCY 1 cut(s) 344
BstHHI GCGC 3 cut(s) 125, 343, 415
BstKTI GATC 3 cut(s) 51, 209, 261
BstMAI GTCTC 3 cut(s) 549, 993, 1152
BstMBI GATC 3 cut(s) 48, 206, 258
BstMCI CGRYCG 5 cut(s) 22, 51, 261, 285, 573
BstMWI GCNNNNNNNGC 4 cut(s) 80, 335, 557, 566
BstNI CCWGG 2 cut(s) 160, 199
BstNSI RCATGY 1 cut(s) 1276
BstSCI CCNGG 2 cut(s) 158, 197
BstSFI CTRYAG 2 cut(s) 600, 1134
BstSLI GKGCMC 1 cut(s) 1173
BstUI CGCG 4 cut(s) 257, 281, 360, 413
BstV1I GCAGC 2 cut(s) 335, 544
BstV2I GAAGAC 1 cut(s) 773
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BstZ17I GTATAC 1 cut(s) 445
BstZI CGGCCG 1 cut(s) 570
BsuRI GGCC 1 cut(s) 572
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 6 cut(s) 570, 695, 1049, 1154, 1257, 1334
BtsIMutI CAGTG 2 cut(s) 727, 1173
Cac8I GCNNGC 4 cut(s) 375, 1012, 1249, 1274
CciI TCATGA 1 cut(s) 1080
CciNI GCGGCCGC 1 cut(s) 570
CfoI GCGC 3 cut(s) 125, 343, 415
Cfr10I RCCGGY 3 cut(s) 21, 32, 269
Cfr13I GGNCC 3 cut(s) 144, 156, 590
CseI GACGC 2 cut(s) 33, 523
Csp6I GTAC 3 cut(s) 362, 693, 1183
CspAI ACCGGT 2 cut(s) 21, 269
CviAII CATG 3 cut(s) 1081, 1090, 1273
CviQI GTAC 3 cut(s) 362, 693, 1183
DdeI CTNAG 1 cut(s) 612
DpnI GATC 3 cut(s) 50, 208, 260
DpnII GATC 3 cut(s) 48, 206, 258
DraI TTTAAA 1 cut(s) 757
EaeI YGGCCR 1 cut(s) 570
EagI CGGCCG 1 cut(s) 570
Eam1104I CTCTTC 1 cut(s) 1113
EarI CTCTTC 1 cut(s) 1113
Ecl136II GAGCTC 1 cut(s) 15
EclXI CGGCCG 1 cut(s) 570
Eco130I CCWWGG 2 cut(s) 1210, 1232
Eco24I GRGCYC 2 cut(s) 17, 1057
Eco32I GATATC 1 cut(s) 229
Eco47I GGWCC 3 cut(s) 144, 156, 590
Eco52I CGGCCG 1 cut(s) 570
Eco53kI GAGCTC 1 cut(s) 15
Eco88I CYCGRG 1 cut(s) 656
EcoICRI GAGCTC 1 cut(s) 15
EcoNI CCTNNNNNAGG 1 cut(s) 151
EcoRII CCWGG 2 cut(s) 158, 197
EcoRV GATATC 1 cut(s) 229
EcoT14I CCWWGG 2 cut(s) 1210, 1232
EcoT22I ATGCAT 1 cut(s) 929
EcoT38I GRGCYC 2 cut(s) 17, 1057
ErhI CCWWGG 2 cut(s) 1210, 1232
Esp3I CGTCTC 2 cut(s) 993, 1152
FaeI CATG 3 cut(s) 1084, 1093, 1276
FatI CATG 3 cut(s) 1080, 1089, 1272
FauNDI CATATG 2 cut(s) 901, 923
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 7 cut(s) 324, 327, 339, 558, 570, 573, 744
FokI GGATG 6 cut(s) 577, 702, 1056, 1141, 1244, 1321
FriOI GRGCYC 2 cut(s) 17, 1057
Fsp4HI GCNGC 7 cut(s) 324, 327, 339, 558, 570, 573, 744
FspBI CTAG 3 cut(s) 554, 845, 1032
GlaI GCGC 3 cut(s) 124, 342, 414
GluI GCNGC 7 cut(s) 324, 327, 339, 558, 570, 573, 744
GsaI CCCAGC 2 cut(s) 347, 564
HaeII RGCGCY 1 cut(s) 344
HaeIII GGCC 1 cut(s) 572
HapII CCGG 5 cut(s) 22, 33, 188, 270, 288
HgaI GACGC 2 cut(s) 33, 523
HhaI GCGC 3 cut(s) 125, 343, 415
Hin1I GRCGYC 2 cut(s) 44, 534
Hin1II CATG 3 cut(s) 1084, 1093, 1276
Hin6I GCGC 3 cut(s) 123, 341, 413
HinP1I GCGC 3 cut(s) 123, 341, 413
HindIII AAGCTT 1 cut(s) 956
HinfI GANTC 6 cut(s) 62, 181, 355, 542, 778, 1313
HpaII CCGG 5 cut(s) 22, 33, 188, 270, 288
HphI GGTGA 1 cut(s) 1320
Hpy166II GTNNAC 3 cut(s) 445, 895, 1183
Hpy188I TCNGA 6 cut(s) 19, 241, 467, 615, 998, 1312
Hpy188III TCNNGA 9 cut(s) 150, 232, 352, 588, 658, 845, 1032, 1081, 1123
Hpy8I GTNNAC 3 cut(s) 445, 895, 1183
Hpy99I CGWCG 2 cut(s) 49, 224
HpyAV CCTTC 6 cut(s) 147, 412, 823, 859, 1043, 1070
HpyCH4III ACNGT 6 cut(s) 366, 604, 823, 972, 1162, 1187
HpyCH4V TGCA 3 cut(s) 927, 1136, 1247
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 335, 557, 566
HpyF3I CTNAG 1 cut(s) 612
Hsp92I GRCGYC 2 cut(s) 44, 534
Hsp92II CATG 3 cut(s) 1084, 1093, 1276
HspAI GCGC 3 cut(s) 123, 341, 413
Kzo9I GATC 3 cut(s) 48, 206, 258
LmnI GCTCC 3 cut(s) 20, 812, 1060
Lsp1109I GCAGC 2 cut(s) 335, 544
LweI GCATC 5 cut(s) 486, 584, 1034, 1163, 1229
MaeI CTAG 3 cut(s) 554, 845, 1032
MaeIII GTNAC 3 cut(s) 68, 275, 484
MalI GATC 3 cut(s) 50, 208, 260
MbiI CCGCTC 1 cut(s) 746
MboI GATC 3 cut(s) 48, 206, 258
MboII GAAGA 6 cut(s) 236, 397, 773, 932, 1055, 1100
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 3 cut(s) 17, 1057, 1173
MluCI AATT 6 cut(s) 250, 480, 525, 633, 909, 1317
MlyI GAGTC 2 cut(s) 551, 787
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 8 cut(s) 157, 169, 195, 396, 419, 654, 1127, 1336
Mph1103I ATGCAT 1 cut(s) 929
MseI TTAA 6 cut(s) 26, 38, 170, 524, 756, 975
MslI CAYNNNNRTG 1 cut(s) 1178
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 5 cut(s) 22, 33, 188, 270, 288
MspR9I CCNGG 2 cut(s) 160, 199
MvaI CCWGG 2 cut(s) 160, 199
MvnI CGCG 4 cut(s) 257, 281, 360, 413
MwoI GCNNNNNNNGC 4 cut(s) 80, 335, 557, 566
NdeI CATATG 2 cut(s) 901, 923
NdeII GATC 3 cut(s) 48, 206, 258
NlaIII CATG 3 cut(s) 1084, 1093, 1276
NlaIV GGNNCC 3 cut(s) 268, 742, 1056
NmuCI GTSAC 1 cut(s) 275
NotI GCGGCCGC 1 cut(s) 570
NsiI ATGCAT 1 cut(s) 929
NspI RCATGY 1 cut(s) 1276
PaeI GCATGC 1 cut(s) 1276
PagI TCATGA 1 cut(s) 1080
PcsI WCGNNNNNNNCGW 4 cut(s) 57, 210, 219, 357
PfeI GAWTC 4 cut(s) 62, 181, 355, 1313
PflMI CCANNNNNTGG 2 cut(s) 165, 1211
PfoI TCCNGGA 1 cut(s) 197
PinAI ACCGGT 2 cut(s) 21, 269
PkrI GCNGC 7 cut(s) 325, 328, 340, 559, 571, 574, 745
Ple19I CGATCG 2 cut(s) 51, 261
PleI GAGTC 2 cut(s) 550, 786
PpsI GAGTC 2 cut(s) 550, 786
PsiI TTATAA 1 cut(s) 1098
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 2 cut(s) 158, 197
PspFI CCCAGC 2 cut(s) 343, 560
PspGI CCWGG 2 cut(s) 158, 197
PspN4I GGNNCC 3 cut(s) 268, 742, 1056
PspPI GGNCC 3 cut(s) 144, 156, 590
PstI CTGCAG 1 cut(s) 1138
PsuI RGATCY 1 cut(s) 206
PvuI CGATCG 2 cut(s) 51, 261
RsaI GTAC 3 cut(s) 363, 694, 1184
RsaNI GTAC 3 cut(s) 362, 693, 1183
RseI CAYNNNNRTG 1 cut(s) 1178
SacI GAGCTC 1 cut(s) 17
SaqAI TTAA 6 cut(s) 26, 38, 170, 524, 756, 975
SatI GCNGC 7 cut(s) 324, 327, 339, 558, 570, 573, 744
Sau3AI GATC 3 cut(s) 48, 206, 258
Sau96I GGNCC 3 cut(s) 144, 156, 590
SchI GAGTC 2 cut(s) 551, 787
ScrFI CCNGG 2 cut(s) 160, 199
SduI GDGCHC 3 cut(s) 17, 1057, 1173
SfaNI GCATC 5 cut(s) 486, 584, 1034, 1163, 1229
SfcI CTRYAG 2 cut(s) 600, 1134
SinI GGWCC 3 cut(s) 144, 156, 590
SmiMI CAYNNNNRTG 1 cut(s) 1178
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
SphI GCATGC 1 cut(s) 1276
Sse9I AATT 6 cut(s) 250, 480, 525, 633, 909, 1317
SsiI CCGC 9 cut(s) 128, 142, 281, 326, 338, 569, 573, 744, 1178
SspI AATATT 2 cut(s) 684, 1024
SspMI CTAG 3 cut(s) 554, 845, 1032
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 2 cut(s) 158, 197
StyI CCWWGG 2 cut(s) 1210, 1232
TaaI ACNGT 6 cut(s) 366, 604, 823, 972, 1162, 1187
TaqI TCGA 6 cut(s) 47, 60, 106, 151, 174, 452
TasI AATT 6 cut(s) 250, 480, 525, 633, 909, 1317
TatI WGTACW 2 cut(s) 692, 1182
TauI GCSGC 5 cut(s) 329, 341, 572, 575, 746
TfiI GAWTC 4 cut(s) 62, 181, 355, 1313
Tru1I TTAA 6 cut(s) 26, 38, 170, 524, 756, 975
Tru9I TTAA 6 cut(s) 26, 38, 170, 524, 756, 975
TscAI CASTG 2 cut(s) 727, 1173
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 2 cut(s) 323, 557
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 4 cut(s) 741, 842, 1100, 1119
TspGWI ACGGA 2 cut(s) 79, 219
TspRI CASTG 2 cut(s) 727, 1173
Van91I CCANNNNNTGG 2 cut(s) 165, 1211
VpaK11BI GGWCC 3 cut(s) 144, 156, 590
XagI CCTNNNNNAGG 1 cut(s) 151
XapI RAATTY 1 cut(s) 633
XbaI TCTAGA 2 cut(s) 844, 1031
XceI RCATGY 1 cut(s) 1276
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XmiI GTMKAC 1 cut(s) 444
XspI CTAG 3 cut(s) 554, 845, 1032
Zsp2I ATGCAT 1 cut(s) 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.