MD03G1165200.v1.1

CRT-like, chloroquine-resistance transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0019133
Physical Location & Seq
Reverse (-)
21858887 .. 21868193
9307 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1165200.v1.1.491

Sequence Viewer

Length: 1368 bp
ATGATATCGAGTATCCACCGCGTATGGGCGGGCGCCGGAGCTCCCGAACCACCAATTTGGATCCGGGAACCACCACGCTTGCAGCCCGAGATCTCACATCTCCGGCACAGATATTACGGGAGTATTCTCCGGCAAGGAATCCGGCTGCGGACGACGCCGTCTGAGCATTTGCAGCGGGTGACATGGACGGTGGAAGCGGTGGGGTCCAGAGGGGCGTGGGAGCGATCGGAGGGCGGGGAAGGAGCGGAGAGCTTGAAGGCGGGGCCGTGCTCGTATGCGGTGGGGGAGTATCGGGCGTCGGTGGAGCTGGAAAGGAGTAGTAAGGATCGGAGGGTGGACGTGGCGGTTGCAGCAGCGGTTACGGTGGTTCTGGGGGTCGGAAATCGGGTGCTCTATAAGCTGGCCTTGGTCCCCCTTAAACACTACCCATTCTTTCTGGCTCAGCTCGCCACTTTCGGATATGTAATTGTATATTTCTCAGTCTTGTATCTCCGGTATCGCGCTGGTATTGTTACCGATGAGATGCTATCCATGCCAAAAGCTCCATTTCTTGCTGTTGGCCTCTTGGAAGCTCTTGGTGCTGCTACTGGGATGGCAGCTGGAGCAATTCTTTCTGGAGCATCAATTCCAGTTTTGTCTCAGACGTTTCTTGTTTGGCAAATTCTCCTGTCAATTATCTTCCTTGGAAGGAGATACAGGGTCAATCAACTTTTAGGGTGCGTTCTTGTAGCCATTGGTGTAATCATAACTGTAGCAAGTGGATCTAGTGCTGGATATTCGTTAAAAGAAGCTGGTGTATTTTGGAGTCTTCTCATGATAGTTTCATTTTTGTTCCAAGCAGCTGATACAGTGCTGAAGGAAGTAATCTTTCTGGATGCTCGTCAGCGGTTAAAGGGAAGATCGGTGGACCTATTTGTTGTAAATTCCTTTGGATCTGCTTTCCAAGCACTATTTATATGCCTCCTCTTACCGTTTTTGTCGAAATTATGGGGGATTCCATTTGGTCAACTGCCAAACTATCTTAAAGACGGTGCAGCTTGCTTTCTAAACATGGGTACTTTATCAACAGGATGTGATGGTGCTCCATTACTTCCGTTACTATTTGTTATTGTCAACATGGGCTTTAACATATCATTGCTGCATCTTCTCAAGATATCTTCTGCTGTGGTATCTTCCCTCGCATCGACATTTTCAGTGCCGATATCTGTGTATGTCTTCACGCTGCCACTACCACACCTAGGTGTTGCAGCCTCGTTGCCCACTGGCTTTGTCTCTGGGACCATTGTTCTTGTAATTGGCTTGCTCATCTATGCCTGGACACCTTCAGTCTATCCAACTAATGCCTCCCCATCACCTCCTACTGTCTGA

Protein Analysis

456

Amino Acids

49.09

Weight (kDa)

9.47

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRT-like PF08627 118 - 437 2.7e-43 CRT-like, chloroquine-resistance transporter-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12170 AT5G12170 AT5G19380 AT5G19380
malus_domestica MD03G1165200.v1.1
prunus_persica Prupe.4G263900_v2.0.a1 Prupe.4G263900_v2.0.a1
pyrus_communis pycom03g11980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 157
AccB1I GGYRCC 1 cut(s) 32
AccBSI CCGCTC 1 cut(s) 245
AccII CGCG 2 cut(s) 21, 501
AclWI GGATC 5 cut(s) 55, 68, 333, 769, 940
AcsI RAATTY 2 cut(s) 660, 922
AcuI CTGAAG 2 cut(s) 875, 1308
AcyI GRCGYC 3 cut(s) 33, 155, 296
AfaI GTAC 1 cut(s) 1057
AfiI CCNNNNNNNGG 2 cut(s) 25, 1238
AgsI TTSAA 1 cut(s) 256
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 1 cut(s) 1313
AleI CACNNNNGTG 1 cut(s) 1239
Alw21I GWGCWC 4 cut(s) 43, 272, 393, 1084
Alw26I GTCTC 2 cut(s) 642, 1276
AlwI GGATC 5 cut(s) 55, 68, 333, 769, 940
Ama87I CYCGRG 1 cut(s) 86
AoxI GGCC 3 cut(s) 263, 402, 559
ApoI RAATTY 2 cut(s) 660, 922
AspA2I CCTAGG 1 cut(s) 1237
AspLEI GCGC 2 cut(s) 35, 503
AspS9I GGNCC 5 cut(s) 204, 263, 409, 907, 1278
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 2 cut(s) 190, 1344
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 4 cut(s) 204, 409, 907, 1278
AvrII CCTAGG 1 cut(s) 1237
BamHI GGATCC 1 cut(s) 60
BanI GGYRCC 1 cut(s) 32
BanII GRGCYC 1 cut(s) 43
BbsI GAAGAC 2 cut(s) 800, 1207
Bbv12I GWGCWC 4 cut(s) 43, 272, 393, 1084
BccI CCATC 3 cut(s) 586, 1070, 1357
BceAI ACGGC 2 cut(s) 142, 250
BciT130I CCWGG 1 cut(s) 1315
BciVI GTATCC 1 cut(s) 23
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 2 cut(s) 642, 1276
BfaI CTAG 2 cut(s) 765, 1238
BfmI CTRYAG 1 cut(s) 750
BfoI RGCGCY 1 cut(s) 36
BfuI GTATCC 1 cut(s) 23
BglII AGATCT 1 cut(s) 90
BlnI CCTAGG 1 cut(s) 1237
BlpI GCTNAGC 1 cut(s) 441
Bme1390I CCNGG 2 cut(s) 65, 1315
Bme18I GGWCC 4 cut(s) 204, 409, 907, 1278
BmeT110I CYCGRG 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 5 cut(s) 204, 263, 409, 907, 1278
BmiI GGNNCC 7 cut(s) 34, 62, 69, 205, 264, 411, 1279
BmrFI CCNGG 2 cut(s) 65, 1315
BmrI ACTGGG 1 cut(s) 597
BmsI GCATC 5 cut(s) 513, 629, 865, 1150, 1190
BmuI ACTGGG 1 cut(s) 597
BpiI GAAGAC 2 cut(s) 800, 1207
BpmI CTGGAG 2 cut(s) 621, 636
Bpu1102I GCTNAGC 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 1133
BpuMI CCSGG 1 cut(s) 65
BsaHI GRCGYC 3 cut(s) 33, 155, 296
BsaJI CCNNGG 3 cut(s) 405, 682, 1237
BsaWI WCCGGW 1 cut(s) 492
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 1238
Bse1I ACTGG 3 cut(s) 592, 629, 1267
Bse3DI GCAATG 1 cut(s) 1133
BseBI CCWGG 1 cut(s) 1315
BseDI CCNNGG 3 cut(s) 405, 682, 1237
BseGI GGATG 3 cut(s) 597, 880, 1076
BseLI CCNNNNNNNGG 2 cut(s) 25, 1238
BseMI GCAATG 1 cut(s) 1133
BseMII CTCAG 4 cut(s) 153, 455, 492, 653
BseNI ACTGG 3 cut(s) 592, 629, 1267
BseRI GAGGAG 1 cut(s) 953
BsgI GTGCAG 1 cut(s) 1053
Bsh1236I CGCG 2 cut(s) 21, 501
Bsh1285I CGRYCG 1 cut(s) 227
BshFI GGCC 3 cut(s) 265, 404, 561
BshNI GGYRCC 1 cut(s) 32
BsiEI CGRYCG 1 cut(s) 227
BsiHKAI GWGCWC 4 cut(s) 43, 272, 393, 1084
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 6 cut(s) 36, 64, 103, 130, 142, 493
BslFI GGGAC 2 cut(s) 395, 1291
BslI CCNNNNNNNGG 2 cut(s) 25, 1238
BsmAI GTCTC 2 cut(s) 642, 1276
BsmFI GGGAC 2 cut(s) 395, 1291
BsnI GGCC 3 cut(s) 265, 404, 561
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 4 cut(s) 43, 272, 393, 1084
Bsp143I GATC 7 cut(s) 60, 90, 224, 325, 761, 899, 932
Bsp1720I GCTNAGC 1 cut(s) 441
BspANI GGCC 3 cut(s) 265, 404, 561
BspCNI CTCAG 4 cut(s) 154, 454, 491, 652
BspFNI CGCG 2 cut(s) 21, 501
BspHI TCATGA 1 cut(s) 813
BspLI GGNNCC 7 cut(s) 34, 62, 69, 205, 264, 411, 1279
BspPI GGATC 5 cut(s) 55, 68, 333, 769, 940
BspT107I GGYRCC 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 1133
BsrI ACTGG 3 cut(s) 592, 629, 1267
BssECI CCNNGG 3 cut(s) 405, 682, 1237
BssMI GATC 7 cut(s) 60, 90, 224, 325, 761, 899, 932
BssNI GRCGYC 3 cut(s) 33, 155, 296
BssT1I CCWWGG 3 cut(s) 405, 682, 1237
Bst2UI CCWGG 1 cut(s) 1315
Bst4CI ACNGT 7 cut(s) 190, 364, 751, 850, 972, 1031, 1363
BstACI GRCGYC 3 cut(s) 33, 155, 296
BstC8I GCNNGC 6 cut(s) 31, 80, 402, 447, 1039, 1301
BstDEI CTNAG 4 cut(s) 162, 441, 478, 639
BstF5I GGATG 3 cut(s) 597, 880, 1076
BstFNI CGCG 2 cut(s) 21, 501
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 2 cut(s) 35, 503
BstKTI GATC 7 cut(s) 63, 93, 227, 328, 764, 902, 935
BstMAI GTCTC 2 cut(s) 642, 1276
BstMBI GATC 7 cut(s) 60, 90, 224, 325, 761, 899, 932
BstMCI CGRYCG 1 cut(s) 227
BstNI CCWGG 1 cut(s) 1315
BstSCI CCNGG 2 cut(s) 63, 1313
BstSFI CTRYAG 1 cut(s) 750
BstUI CGCG 2 cut(s) 21, 501
BstV2I GAAGAC 2 cut(s) 800, 1207
BstX2I RGATCY 4 cut(s) 60, 90, 761, 932
BstXI CCANNNNNNTGG 1 cut(s) 57
BstYI RGATCY 4 cut(s) 60, 90, 761, 932
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 3 cut(s) 265, 404, 561
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 3 cut(s) 597, 880, 1076
BtsIMutI CAGTG 3 cut(s) 855, 1200, 1260
Cac8I GCNNGC 6 cut(s) 31, 80, 402, 447, 1039, 1301
CciI TCATGA 1 cut(s) 813
CfoI GCGC 2 cut(s) 35, 503
Cfr13I GGNCC 5 cut(s) 204, 263, 409, 907, 1278
CseI GACGC 2 cut(s) 163, 285
Csp6I GTAC 1 cut(s) 1056
CspCI CAANNNNNGTGG 4 cut(s) 60, 95, 1249, 1284
CviAII CATG 5 cut(s) 183, 532, 814, 1051, 1117
CviQI GTAC 1 cut(s) 1056
DdeI CTNAG 4 cut(s) 162, 441, 478, 639
DinI GGCGCC 1 cut(s) 34
DpnI GATC 7 cut(s) 62, 92, 226, 327, 763, 901, 934
DpnII GATC 7 cut(s) 60, 90, 224, 325, 761, 899, 932
DrdI GACNNNNNNGTC 1 cut(s) 157
DseDI GACNNNNNNGTC 1 cut(s) 157
Ecl136II GAGCTC 1 cut(s) 41
Eco130I CCWWGG 3 cut(s) 405, 682, 1237
Eco24I GRGCYC 1 cut(s) 43
Eco32I GATATC 3 cut(s) 6, 1155, 1203
Eco47I GGWCC 4 cut(s) 204, 409, 907, 1278
Eco53kI GAGCTC 1 cut(s) 41
Eco57I CTGAAG 2 cut(s) 875, 1308
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 1313
EcoRV GATATC 3 cut(s) 6, 1155, 1203
EcoT14I CCWWGG 3 cut(s) 405, 682, 1237
EcoT38I GRGCYC 1 cut(s) 43
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 3 cut(s) 405, 682, 1237
FaeI CATG 5 cut(s) 186, 535, 817, 1054, 1120
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FaqI GGGAC 2 cut(s) 395, 1291
FatI CATG 5 cut(s) 182, 531, 813, 1050, 1116
FauI CCCGC 4 cut(s) 22, 168, 227, 253
FokI GGATG 3 cut(s) 604, 887, 1083
FriOI GRGCYC 1 cut(s) 43
FspBI CTAG 2 cut(s) 765, 1238
GlaI GCGC 2 cut(s) 34, 502
GsuI CTGGAG 2 cut(s) 621, 636
HaeII RGCGCY 1 cut(s) 36
HaeIII GGCC 3 cut(s) 265, 404, 561
HapII CCGG 6 cut(s) 36, 64, 103, 130, 142, 493
HgaI GACGC 2 cut(s) 163, 285
HhaI GCGC 2 cut(s) 35, 503
Hin1I GRCGYC 3 cut(s) 33, 155, 296
Hin1II CATG 5 cut(s) 186, 535, 817, 1054, 1120
Hin6I GCGC 2 cut(s) 33, 501
HinP1I GCGC 2 cut(s) 33, 501
HincII GTYRAC 2 cut(s) 1007, 1114
HindII GTYRAC 2 cut(s) 1007, 1114
HinfI GANTC 3 cut(s) 138, 805, 994
HpaII CCGG 6 cut(s) 36, 64, 103, 130, 142, 493
HphI GGTGA 2 cut(s) 190, 1344
Hpy166II GTNNAC 4 cut(s) 337, 907, 1007, 1114
Hpy188I TCNGA 7 cut(s) 163, 229, 330, 380, 458, 642, 1367
Hpy188III TCNNGA 6 cut(s) 44, 207, 615, 814, 872, 1150
Hpy8I GTNNAC 4 cut(s) 337, 907, 1007, 1114
Hpy99I CGWCG 2 cut(s) 157, 301
HpyAV CCTTC 5 cut(s) 233, 250, 681, 850, 1332
HpyCH4III ACNGT 7 cut(s) 190, 364, 751, 850, 972, 1031, 1363
HpyCH4IV ACGT 2 cut(s) 339, 644
HpyCH4V TGCA 6 cut(s) 82, 172, 350, 1034, 1141, 1247
HpyF3I CTNAG 4 cut(s) 162, 441, 478, 639
HpySE526I ACGT 2 cut(s) 339, 644
Hsp92I GRCGYC 3 cut(s) 33, 155, 296
Hsp92II CATG 5 cut(s) 186, 535, 817, 1054, 1120
HspAI GCGC 2 cut(s) 33, 501
KasI GGCGCC 1 cut(s) 32
Kzo9I GATC 7 cut(s) 60, 90, 224, 325, 761, 899, 932
LmnI GCTCC 9 cut(s) 38, 46, 220, 242, 304, 547, 602, 617, 1087
LweI GCATC 5 cut(s) 513, 629, 865, 1150, 1190
MaeI CTAG 2 cut(s) 765, 1238
MaeII ACGT 2 cut(s) 339, 644
MaeIII GTNAC 4 cut(s) 178, 358, 511, 1095
MalI GATC 7 cut(s) 62, 92, 226, 327, 763, 901, 934
MbiI CCGCTC 1 cut(s) 245
MboI GATC 7 cut(s) 60, 90, 224, 325, 761, 899, 932
MboII GAAGA 7 cut(s) 670, 800, 909, 1136, 1149, 1164, 1207
MflI RGATCY 4 cut(s) 60, 90, 761, 932
MhlI GDGCHC 4 cut(s) 43, 272, 393, 1084
MluCI AATT 9 cut(s) 54, 465, 606, 624, 660, 672, 922, 983, 1293
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 1 cut(s) 814
MmeI TCCRAC 2 cut(s) 358, 1358
MseI TTAA 5 cut(s) 417, 782, 890, 1023, 1125
MslI CAYNNNNRTG 1 cut(s) 1239
MspA1I CMGCKG 5 cut(s) 175, 356, 599, 842, 886
MspI CCGG 6 cut(s) 36, 64, 103, 130, 142, 493
MspR9I CCNGG 2 cut(s) 65, 1315
MvaI CCWGG 1 cut(s) 1315
MvnI CGCG 2 cut(s) 21, 501
NarI GGCGCC 1 cut(s) 33
NciI CCSGG 1 cut(s) 65
NdeII GATC 7 cut(s) 60, 90, 224, 325, 761, 899, 932
NlaIII CATG 5 cut(s) 186, 535, 817, 1054, 1120
NlaIV GGNNCC 7 cut(s) 34, 62, 69, 205, 264, 411, 1279
NmuCI GTSAC 1 cut(s) 178
OliI CACNNNNGTG 1 cut(s) 1239
PagI TCATGA 1 cut(s) 813
PfeI GAWTC 2 cut(s) 138, 994
PflFI GACNNNGTC 1 cut(s) 157
PfoI TCCNGGA 1 cut(s) 63
Ple19I CGATCG 1 cut(s) 227
PleI GAGTC 1 cut(s) 813
PluTI GGCGCC 1 cut(s) 36
PpsI GAGTC 1 cut(s) 813
Psp124BI GAGCTC 1 cut(s) 43
Psp6I CCWGG 1 cut(s) 1313
PspGI CCWGG 1 cut(s) 1313
PspN4I GGNNCC 7 cut(s) 34, 62, 69, 205, 264, 411, 1279
PspPI GGNCC 5 cut(s) 204, 263, 409, 907, 1278
PsuI RGATCY 4 cut(s) 60, 90, 761, 932
PsyI GACNNNGTC 1 cut(s) 157
PvuI CGATCG 1 cut(s) 227
PvuII CAGCTG 2 cut(s) 599, 842
RsaI GTAC 1 cut(s) 1057
RsaNI GTAC 1 cut(s) 1056
RseI CAYNNNNRTG 1 cut(s) 1239
SacI GAGCTC 1 cut(s) 43
SaqAI TTAA 5 cut(s) 417, 782, 890, 1023, 1125
Sau3AI GATC 7 cut(s) 60, 90, 224, 325, 761, 899, 932
Sau96I GGNCC 5 cut(s) 204, 263, 409, 907, 1278
SchI GAGTC 1 cut(s) 814
ScrFI CCNGG 2 cut(s) 65, 1315
SduI GDGCHC 4 cut(s) 43, 272, 393, 1084
SfaNI GCATC 5 cut(s) 513, 629, 865, 1150, 1190
SfcI CTRYAG 1 cut(s) 750
SfoI GGCGCC 1 cut(s) 34
SinI GGWCC 4 cut(s) 204, 409, 907, 1278
SmiMI CAYNNNNRTG 1 cut(s) 1239
SmlI CTYRAG 1 cut(s) 1148
SmoI CTYRAG 1 cut(s) 1148
Sse9I AATT 9 cut(s) 54, 465, 606, 624, 660, 672, 922, 983, 1293
SspDI GGCGCC 1 cut(s) 32
SspMI CTAG 2 cut(s) 765, 1238
SstI GAGCTC 1 cut(s) 43
StyD4I CCNGG 2 cut(s) 63, 1313
StyI CCWWGG 3 cut(s) 405, 682, 1237
TaaI ACNGT 7 cut(s) 190, 364, 751, 850, 972, 1031, 1363
TaiI ACGT 2 cut(s) 342, 647
TaqI TCGA 3 cut(s) 8, 980, 1184
TasI AATT 9 cut(s) 54, 465, 606, 624, 660, 672, 922, 983, 1293
TfiI GAWTC 2 cut(s) 138, 994
Tru1I TTAA 5 cut(s) 417, 782, 890, 1023, 1125
Tru9I TTAA 5 cut(s) 417, 782, 890, 1023, 1125
TscAI CASTG 3 cut(s) 855, 1200, 1267
TseFI GTSAC 1 cut(s) 178
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 1 cut(s) 813
TspGWI ACGGA 1 cut(s) 1083
TspRI CASTG 3 cut(s) 855, 1200, 1267
Tth111I GACNNNGTC 1 cut(s) 157
VpaK11BI GGWCC 4 cut(s) 204, 409, 907, 1278
XapI RAATTY 2 cut(s) 660, 922
XmaJI CCTAGG 1 cut(s) 1237
XspI CTAG 2 cut(s) 765, 1238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.