Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0019559

Physical Location & Seq

Genomic Coordinates Reverse (-)

6836781 .. 6839609

Gene Structure 2829 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G20260.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1005 bp

ATGGAGAAGAAATTCAAAGTGTGGGTGTACAGAGAAGGAGAGACACCGTTAGTTCACATGGGTCCAATGAACAACATATACAGCATTGAAGGCCAATTCATGGACGAGATCGAGACAGGGATGAGCCCGTTCGCGGCTAACAACCCCGAAGAGGCTCATGCCTTTCTCCTCCCTGTCAGCGTCGCCAACATCGTTCATTACCTATACCGGCCGCTCGTGACCTACTCGAGGGAACAACTTCACAAAGTTTTTCTTGACTACGTCGATGTTGTGGCTCACAAGTATCCTTATTGGAACAGAAGCCTTGGAGCCGACCATTTCTACGTTTCTTGCCATGATTGGGCACCAGACGTCTCAGGATCGAACCCGGAGCTGATGAAAAACCTGATAAGAGTTCTCTGCAACGCCAACACATCAGAAGGTTTCATGCCCCAACGAGACGTCTCAATCCCGGAGATCAACATCCCCGGAGGCCACCTGGGACCACCTCGGTTATCGCGCTCTTCGGGCCATGACCGACCCATCCTTGCTTTCTTCGCAGGGGGTTCACACGGTTATATCCGCAGAATTCTATTGCAGCATTGGAAAGACAAAGATGAGGAGGTCCAAGTCCACGAGTACTTAGCCAAAAACAAAGACTACTTCAAGCTCATGGCTACGGCAAGGTTCTGTCTCTGTCCTAGTGGCTACGAAGTGGCTAGCCCTCGAGTCGTTGCAGCCATTAACTTAGGTTGCGTCCCTGTCATCATCTCTGATCACTACGCCTTGCCTTTCTCAGACGTTCTTGATTGGACTAAGTTCACCATTCATGTTCCTTCTAAGAAGATCCCTGAGATCAAAACCATACTAAAGAGCATATCCTGGAGACGCTACAGGGTGTTGCAGAGAAGGGTTTTGCAGGTCCAGAGACATTTCGTGATCAACAGACCGTCACAACCGTTTGATATGCTTAGAATGCTTCTTCACTCTGTTTGGTTGAGAAGACTAAACCTGAGGTTGCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009987 GO:0010393 GO:0010398 GO:0012505 GO:0016020 GO:0016051 GO:0016740 GO:0016757 GO:0016763 GO:0017144 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0035252 GO:0042285 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045488 GO:0045489 GO:0071704 GO:0097708 GO:0098588 GO:0098791 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Exostosin

Protein Analysis

334

Amino Acids

38.68

Weight (kDa)

9.17

Isoelectric Point (pI)

58.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exostosin_GT47	PF03016	134 - 418	1.7e-60	Exostosin GT47 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019559)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20260 AT5G20260 AT5G20260
malus_domestica	MD02G1022700.v1.1 MD15G1165400.v1.1
prunus_persica	Prupe.7G249400_v2.0.a1
pyrus_communis	pycom15g14810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	354, 444
Acc36I	ACCTGC	1 cut(s)	889
AccB1I	GGYRCC	1 cut(s)	343
AccB7I	CCANNNNNTGG	1 cut(s)	100
AccBSI	CCGCTC	1 cut(s)	214
AccII	CGCG	2 cut(s)	134, 499
AciI	CCGC	3 cut(s)	134, 212, 562
AclWI	GGATC	2 cut(s)	367, 820
AcoI	YGGCCR	1 cut(s)	209
AcsI	RAATTY	2 cut(s)	11, 567
AcyI	GRCGYC	2 cut(s)	351, 441
AfaI	GTAC	2 cut(s)	29, 620
AfiI	CCNNNNNNNGG	5 cut(s)	100, 133, 151, 228, 340
AgsI	TTSAA	3 cut(s)	16, 89, 646
AjnI	CCWGG	2 cut(s)	477, 860
AluBI	AGCT	2 cut(s)	373, 649
AluI	AGCT	2 cut(s)	373, 649
Alw26I	GTCTC	8 cut(s)	35, 107, 358, 432, 448, 677, 859, 901
AlwI	GGATC	2 cut(s)	367, 820
Ama87I	CYCGRG	2 cut(s)	226, 705
AoxI	GGCC	4 cut(s)	91, 209, 472, 508
ApeKI	GCWGC	2 cut(s)	577, 716
ApoI	RAATTY	2 cut(s)	11, 567
Asp700I	GAANNNNTTC	2 cut(s)	11, 237
AspLEI	GCGC	1 cut(s)	501
AspS9I	GGNCC	5 cut(s)	62, 482, 508, 604, 901
AsuC2I	CCSGG	3 cut(s)	368, 452, 468
AsuHPI	GGTGA	1 cut(s)	793
AsuNHI	GCTAGC	1 cut(s)	698
AvaI	CYCGRG	2 cut(s)	226, 705
AvaII	GGWCC	4 cut(s)	62, 482, 604, 901
AxyI	CCTNAGG	1 cut(s)	992
BaeGI	GKGCMC	1 cut(s)	346
BanI	GGYRCC	1 cut(s)	343
BanII	GRGCYC	1 cut(s)	128
BauI	CACGAG	2 cut(s)	215, 614
BbsI	GAAGAC	1 cut(s)	988
BbvI	GCAGC	2 cut(s)	589, 728
BccI	CCATC	1 cut(s)	530
BceAI	ACGGC	1 cut(s)	675
BciT130I	CCWGG	2 cut(s)	479, 862
BciVI	GTATCC	1 cut(s)	294
BclI	TGATCA	2 cut(s)	754, 918
BcnI	CCSGG	3 cut(s)	368, 452, 468
BcoDI	GTCTC	8 cut(s)	35, 107, 358, 432, 448, 677, 859, 901
BfaI	CTAG	2 cut(s)	681, 699
BfmI	CTRYAG	1 cut(s)	871
BfuAI	ACCTGC	1 cut(s)	889
BfuI	GTATCC	1 cut(s)	294
BisI	GCNGC	4 cut(s)	135, 212, 578, 717
BlsI	GCNGC	4 cut(s)	136, 213, 579, 718
BmcAI	AGTACT	1 cut(s)	620
Bme1390I	CCNGG	5 cut(s)	368, 452, 468, 479, 862
Bme18I	GGWCC	4 cut(s)	62, 482, 604, 901
BmeT110I	CYCGRG	2 cut(s)	226, 705
BmgT120I	GGNCC	5 cut(s)	62, 482, 508, 604, 901
BmiI	GGNNCC	4 cut(s)	63, 310, 345, 483
BmrFI	CCNGG	5 cut(s)	368, 452, 468, 479, 862
BmtI	GCTAGC	1 cut(s)	702
BpiI	GAAGAC	1 cut(s)	988
BpmI	CTGGAG	1 cut(s)	883
BpuMI	CCSGG	3 cut(s)	368, 452, 468
BsaBI	GATNNNNATC	1 cut(s)	461
BsaHI	GRCGYC	2 cut(s)	351, 441
BsaJI	CCNNGG	4 cut(s)	304, 466, 478, 488
Bsc4I	CCNNNNNNNGG	5 cut(s)	100, 133, 151, 228, 340
Bse118I	RCCGGY	1 cut(s)	207
Bse21I	CCTNAGG	1 cut(s)	992
Bse8I	GATNNNNATC	1 cut(s)	461
BseBI	CCWGG	2 cut(s)	479, 862
BseDI	CCNNGG	4 cut(s)	304, 466, 478, 488
BseGI	GGATG	3 cut(s)	126, 462, 522
BseJI	GATNNNNATC	1 cut(s)	461
BseLI	CCNNNNNNNGG	5 cut(s)	100, 133, 151, 228, 340
BseMII	CTCAG	4 cut(s)	369, 789, 822, 983
BseRI	GAGGAG	2 cut(s)	158, 614
BseSI	GKGCMC	1 cut(s)	346
BseX3I	CGGCCG	1 cut(s)	209
BseXI	GCAGC	2 cut(s)	589, 728
Bsh1236I	CGCG	2 cut(s)	134, 499
Bsh1285I	CGRYCG	1 cut(s)	212
BshFI	GGCC	4 cut(s)	93, 211, 474, 510
BshNI	GGYRCC	1 cut(s)	343
BsiEI	CGRYCG	1 cut(s)	212
BsiHKCI	CYCGRG	2 cut(s)	226, 705
BsiSI	CCGG	4 cut(s)	208, 368, 452, 468
BslFI	GGGAC	2 cut(s)	495, 722
BslI	CCNNNNNNNGG	5 cut(s)	100, 133, 151, 228, 340
BsmAI	GTCTC	8 cut(s)	35, 107, 358, 432, 448, 677, 859, 901
BsmBI	CGTCTC	4 cut(s)	358, 432, 448, 859
BsmFI	GGGAC	2 cut(s)	495, 722
BsmI	GAATGC	1 cut(s)	960
BsnI	GGCC	4 cut(s)	93, 211, 474, 510
BsoBI	CYCGRG	2 cut(s)	226, 705
Bsp1286I	GDGCHC	2 cut(s)	128, 346
Bsp1407I	TGTACA	1 cut(s)	27
Bsp143I	GATC	7 cut(s)	108, 359, 456, 754, 825, 834, 918
BspACI	CCGC	3 cut(s)	134, 212, 562
BspANI	GGCC	4 cut(s)	93, 211, 474, 510
BspCNI	CTCAG	4 cut(s)	368, 788, 823, 984
BspFNI	CGCG	2 cut(s)	134, 499
BspLI	GGNNCC	4 cut(s)	63, 310, 345, 483
BspMI	ACCTGC	1 cut(s)	889
BspOI	GCTAGC	1 cut(s)	702
BspPI	GGATC	2 cut(s)	367, 820
BspQI	GCTCTTC	1 cut(s)	508
BspT107I	GGYRCC	1 cut(s)	343
BsrBI	CCGCTC	1 cut(s)	214
BsrFI	RCCGGY	1 cut(s)	207
BsrGI	TGTACA	1 cut(s)	27
BssAI	RCCGGY	1 cut(s)	207
BssECI	CCNNGG	4 cut(s)	304, 466, 478, 488
BssMI	GATC	7 cut(s)	108, 359, 456, 754, 825, 834, 918
BssNI	GRCGYC	2 cut(s)	351, 441
BssSI	CACGAG	2 cut(s)	215, 614
BssT1I	CCWWGG	1 cut(s)	304
Bst2BI	CACGAG	2 cut(s)	215, 614
Bst2UI	CCWGG	2 cut(s)	479, 862
Bst4CI	ACNGT	4 cut(s)	48, 554, 930, 939
Bst6I	CTCTTC	2 cut(s)	144, 508
BstACI	GRCGYC	2 cut(s)	351, 441
BstAUI	TGTACA	1 cut(s)	27
BstC8I	GCNNGC	1 cut(s)	700
BstENI	CCTNNNNNAGG	1 cut(s)	226
BstF5I	GGATG	3 cut(s)	126, 462, 522
BstFNI	CGCG	2 cut(s)	134, 499
BstHHI	GCGC	1 cut(s)	501
BstKTI	GATC	7 cut(s)	111, 362, 459, 757, 828, 837, 921
BstMAI	GTCTC	8 cut(s)	35, 107, 358, 432, 448, 677, 859, 901
BstMBI	GATC	7 cut(s)	108, 359, 456, 754, 825, 834, 918
BstMCI	CGRYCG	1 cut(s)	212
BstMWI	GCNNNNNNNGC	4 cut(s)	90, 507, 536, 955
BstNI	CCWGG	2 cut(s)	479, 862
BstSCI	CCNGG	5 cut(s)	366, 450, 466, 477, 860
BstSFI	CTRYAG	1 cut(s)	871
BstSLI	GKGCMC	1 cut(s)	346
BstUI	CGCG	2 cut(s)	134, 499
BstV1I	GCAGC	2 cut(s)	589, 728
BstV2I	GAAGAC	1 cut(s)	988
BstX2I	RGATCY	1 cut(s)	825
BstYI	RGATCY	1 cut(s)	825
BstZI	CGGCCG	1 cut(s)	209
Bsu36I	CCTNAGG	1 cut(s)	992
BsuI	GTATCC	1 cut(s)	294
BsuRI	GGCC	4 cut(s)	93, 211, 474, 510
BtsCI	GGATG	3 cut(s)	126, 462, 522
BveI	ACCTGC	1 cut(s)	889
Cac8I	GCNNGC	1 cut(s)	700
CfoI	GCGC	1 cut(s)	501
Cfr10I	RCCGGY	1 cut(s)	207
Cfr13I	GGNCC	5 cut(s)	62, 482, 508, 604, 901
CseI	GACGC	3 cut(s)	169, 724, 876
Csp6I	GTAC	2 cut(s)	28, 619
CviAII	CATG	8 cut(s)	58, 100, 158, 335, 427, 512, 652, 809
CviQI	GTAC	2 cut(s)	28, 619
DpnI	GATC	7 cut(s)	110, 361, 458, 756, 827, 836, 920
DpnII	GATC	7 cut(s)	108, 359, 456, 754, 825, 834, 918
EaeI	YGGCCR	1 cut(s)	209
EagI	CGGCCG	1 cut(s)	209
Eam1104I	CTCTTC	2 cut(s)	144, 508
EarI	CTCTTC	2 cut(s)	144, 508
EclXI	CGGCCG	1 cut(s)	209
Eco130I	CCWWGG	1 cut(s)	304
Eco24I	GRGCYC	1 cut(s)	128
Eco47I	GGWCC	4 cut(s)	62, 482, 604, 901
Eco52I	CGGCCG	1 cut(s)	209
Eco81I	CCTNAGG	1 cut(s)	992
Eco88I	CYCGRG	2 cut(s)	226, 705
EcoNI	CCTNNNNNAGG	1 cut(s)	226
EcoRI	GAATTC	1 cut(s)	567
EcoRII	CCWGG	2 cut(s)	477, 860
EcoT14I	CCWWGG	1 cut(s)	304
EcoT38I	GRGCYC	1 cut(s)	128
ErhI	CCWWGG	1 cut(s)	304
Esp3I	CGTCTC	4 cut(s)	358, 432, 448, 859
FaeI	CATG	8 cut(s)	61, 103, 161, 338, 430, 515, 655, 812
FalI	AAGNNNNNCTT	2 cut(s)	237, 269
FaqI	GGGAC	2 cut(s)	495, 722
FatI	CATG	8 cut(s)	57, 99, 157, 334, 426, 511, 651, 808
FbaI	TGATCA	2 cut(s)	754, 918
Fnu4HI	GCNGC	4 cut(s)	135, 212, 578, 717
FokI	GGATG	3 cut(s)	133, 449, 509
FriOI	GRGCYC	1 cut(s)	128
Fsp4HI	GCNGC	4 cut(s)	135, 212, 578, 717
FspBI	CTAG	2 cut(s)	681, 699
GlaI	GCGC	1 cut(s)	500
GluI	GCNGC	4 cut(s)	135, 212, 578, 717
GsuI	CTGGAG	1 cut(s)	883
HaeIII	GGCC	4 cut(s)	93, 211, 474, 510
HapII	CCGG	4 cut(s)	208, 368, 452, 468
HgaI	GACGC	3 cut(s)	169, 724, 876
HhaI	GCGC	1 cut(s)	501
Hin1I	GRCGYC	2 cut(s)	351, 441
Hin1II	CATG	8 cut(s)	61, 103, 161, 338, 430, 515, 655, 812
Hin6I	GCGC	1 cut(s)	499
HinP1I	GCGC	1 cut(s)	499
HinfI	GANTC	1 cut(s)	708
HpaII	CCGG	4 cut(s)	208, 368, 452, 468
HphI	GGTGA	1 cut(s)	793
Hpy166II	GTNNAC	5 cut(s)	28, 55, 548, 613, 801
Hpy188I	TCNGA	3 cut(s)	418, 754, 778
Hpy188III	TCNNGA	7 cut(s)	112, 217, 254, 357, 785, 904, 916
Hpy8I	GTNNAC	5 cut(s)	28, 55, 548, 613, 801
Hpy99I	CGWCG	2 cut(s)	185, 266
HpyAV	CCTTC	5 cut(s)	29, 83, 413, 825, 882
HpyCH4III	ACNGT	4 cut(s)	48, 554, 930, 939
HpyCH4IV	ACGT	5 cut(s)	261, 324, 351, 441, 780
HpyCH4V	TGCA	5 cut(s)	402, 577, 716, 883, 898
HpyF10VI	GCNNNNNNNGC	4 cut(s)	90, 507, 536, 955
HpySE526I	ACGT	5 cut(s)	261, 324, 351, 441, 780
Hsp92I	GRCGYC	2 cut(s)	351, 441
Hsp92II	CATG	8 cut(s)	61, 103, 161, 338, 430, 515, 655, 812
HspAI	GCGC	1 cut(s)	499
Ksp22I	TGATCA	2 cut(s)	754, 918
Kzo9I	GATC	7 cut(s)	108, 359, 456, 754, 825, 834, 918
LguI	GCTCTTC	1 cut(s)	508
LmnI	GCTCC	2 cut(s)	308, 370
Lsp1109I	GCAGC	2 cut(s)	589, 728
MaeI	CTAG	2 cut(s)	681, 699
MaeII	ACGT	5 cut(s)	261, 324, 351, 441, 780
MaeIII	GTNAC	2 cut(s)	217, 930
MalI	GATC	7 cut(s)	110, 361, 458, 756, 827, 836, 920
MbiI	CCGCTC	1 cut(s)	214
MboI	GATC	7 cut(s)	108, 359, 456, 754, 825, 834, 918
MboII	GAAGA	7 cut(s)	19, 161, 495, 526, 835, 953, 993
MflI	RGATCY	1 cut(s)	825
MhlI	GDGCHC	2 cut(s)	128, 346
MluCI	AATT	3 cut(s)	11, 95, 567
MlyI	GAGTC	1 cut(s)	717
MnlI	CCTC	9 cut(s)	145, 179, 222, 464, 498, 592, 595, 714, 987
MroXI	GAANNNNTTC	2 cut(s)	11, 237
MseI	TTAA	1 cut(s)	723
MspI	CCGG	4 cut(s)	208, 368, 452, 468
MspR9I	CCNGG	5 cut(s)	368, 452, 468, 479, 862
Mva1269I	GAATGC	1 cut(s)	960
MvaI	CCWGG	2 cut(s)	479, 862
MvnI	CGCG	2 cut(s)	134, 499
MwoI	GCNNNNNNNGC	4 cut(s)	90, 507, 536, 955
NciI	CCSGG	3 cut(s)	368, 452, 468
NdeII	GATC	7 cut(s)	108, 359, 456, 754, 825, 834, 918
NheI	GCTAGC	1 cut(s)	698
NlaIII	CATG	8 cut(s)	61, 103, 161, 338, 430, 515, 655, 812
NlaIV	GGNNCC	4 cut(s)	63, 310, 345, 483
NmuCI	GTSAC	2 cut(s)	217, 930
PaeR7I	CTCGAG	2 cut(s)	226, 705
PciSI	GCTCTTC	1 cut(s)	508
PcsI	WCGNNNNNNNCGW	1 cut(s)	189
PctI	GAATGC	1 cut(s)	960
PdmI	GAANNNNTTC	2 cut(s)	11, 237
PflFI	GACNNNGTC	1 cut(s)	260
PflMI	CCANNNNNTGG	1 cut(s)	100
PfoI	TCCNGGA	2 cut(s)	450, 860
PkrI	GCNGC	4 cut(s)	136, 213, 579, 718
PleI	GAGTC	1 cut(s)	716
PpsI	GAGTC	1 cut(s)	716
Psp6I	CCWGG	2 cut(s)	477, 860
PspGI	CCWGG	2 cut(s)	477, 860
PspN4I	GGNNCC	4 cut(s)	63, 310, 345, 483
PspPI	GGNCC	5 cut(s)	62, 482, 508, 604, 901
PspXI	VCTCGAGB	2 cut(s)	226, 705
PsrI	GAACNNNNNNTAC	2 cut(s)	62, 94
PsuI	RGATCY	1 cut(s)	825
PsyI	GACNNNGTC	1 cut(s)	260
RsaI	GTAC	2 cut(s)	29, 620
RsaNI	GTAC	2 cut(s)	28, 619
SapI	GCTCTTC	1 cut(s)	508
SaqAI	TTAA	1 cut(s)	723
SatI	GCNGC	4 cut(s)	135, 212, 578, 717
Sau3AI	GATC	7 cut(s)	108, 359, 456, 754, 825, 834, 918
Sau96I	GGNCC	5 cut(s)	62, 482, 508, 604, 901
ScaI	AGTACT	1 cut(s)	620
SchI	GAGTC	1 cut(s)	717
ScrFI	CCNGG	5 cut(s)	368, 452, 468, 479, 862
SduI	GDGCHC	2 cut(s)	128, 346
SfcI	CTRYAG	1 cut(s)	871
Sfr274I	CTCGAG	2 cut(s)	226, 705
SinI	GGWCC	4 cut(s)	62, 482, 604, 901
SlaI	CTCGAG	2 cut(s)	226, 705
SmlI	CTYRAG	2 cut(s)	226, 705
SmoI	CTYRAG	2 cut(s)	226, 705
Sse9I	AATT	3 cut(s)	11, 95, 567
SsiI	CCGC	3 cut(s)	134, 212, 562
SspMI	CTAG	2 cut(s)	681, 699
StyD4I	CCNGG	5 cut(s)	366, 450, 466, 477, 860
StyI	CCWWGG	1 cut(s)	304
TaaI	ACNGT	4 cut(s)	48, 554, 930, 939
TaiI	ACGT	5 cut(s)	264, 327, 354, 444, 783
TaqI	TCGA	5 cut(s)	111, 227, 264, 362, 706
TaqII	GACCGA	1 cut(s)	531
TasI	AATT	3 cut(s)	11, 95, 567
TatI	WGTACW	2 cut(s)	27, 618
TauI	GCSGC	2 cut(s)	137, 214
Tru1I	TTAA	1 cut(s)	723
Tru9I	TTAA	1 cut(s)	723
TseFI	GTSAC	2 cut(s)	217, 930
TseI	GCWGC	2 cut(s)	577, 716
Tsp45I	GTSAC	2 cut(s)	217, 930
TspDTI	ATGAA	6 cut(s)	83, 88, 185, 392, 415, 797
Tth111I	GACNNNGTC	1 cut(s)	260
Van91I	CCANNNNNTGG	1 cut(s)	100
VpaK11BI	GGWCC	4 cut(s)	62, 482, 604, 901
XagI	CCTNNNNNAGG	1 cut(s)	226
XapI	RAATTY	2 cut(s)	11, 567
XhoI	CTCGAG	2 cut(s)	226, 705
XmnI	GAANNNNTTC	2 cut(s)	11, 237
XspI	CTAG	2 cut(s)	681, 699
ZraI	GACGTC	2 cut(s)	352, 442
ZrmI	AGTACT	1 cut(s)	620

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.