MD02G1022700.v1.1

glycosyltransferase At5g20260

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0019559
Physical Location & Seq
Forward (+)
1585051 .. 1587896
2846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1022700.v1.1.491

Sequence Viewer

Length: 1509 bp
ATGGCAGAACTTGGCTCTTCTACTCACATCGTTACTGCACTGCTACCAAGTCTTTCAATTATCCTTTTATTTATCTTTTTCTTATCTCCATTAAACCAAACCAACCTTACCAATATTATTTTCTCGTCTCCTACTCCCATTTCCGGCATCAAAATTATCCATGACACCCCTCCACCCATTTCATCACCTCCTTCTTTGCCATCCTCACCCATTTCACCACCTCCGTCTCCGCCACCATTGTTGCCATCATATTTGTTGCCAACCGATGAATCAAGGAGTATCAATACTTGGATAGTGAAGGAGGAACGCAAAAAAGTGCGCCGTGCAATAAAAATGAGTAATTTGGAGAGAATTGAAGCAGGTTTGGCCAGAGCAAGAGAGGCGATCTGGAGAGCAGTACATGAAAAGAATCAAGCGTCTAATTACAAAGAAGACGAAGAAGTGTTCATCCCAAAAGGATCAATTTACAGAAATGCACATTGTTTTCATCAGAGTTACGCAGAGATGGAGAAAAGATTGAAGGTGTGGGTTTACAGGGAAGGAGAGCATCCCTTGGTTCACACCGGGCCCTTCAACAACATTTATAGTGTTGAGGGGCTGTTTATCGACGAGATGGAGAGCAGGAAGAGCCGGTTCATGGCTCGCCATCCCGACGAGGCACACCTATTCCTCATCCCAATCAGTGTATCCAACATCGTCAATTTTCTGTACAGGCCTCTTGTCACATATAACCGTGATCAACTTCAAAGGGTTGTTATGGACTATATTGGCGTTGTAGCTGAAAAATACCCTTATTGGAATAGAAGCCACGGTGCTGATCATTTCTTGGTTTCTTGTCATGATTGGGCACCAGACATTTCACAAGCTAATCCCAAGTTCTACCAGAACTTCATTAGGGTTTTATGCAATGCCAATACATCAGAAGGGTTTAAGTCCCAAAGAGACGCCTCCATTCCAGAAATCAACATACCATATGGAGAACTTGGTCCGACCCCGCAAAATACAAGCCTCGACAAGGGTTCAATCCTTGCCTTCTTCGCCGGAGGATCTCACGGTCTCATAAGAAAGATCTTGCTGGAGCATTGGAAGGACAAAGACAATGAGGTGCAAGTGCATGAGTACCTAGAAAATAAGGAAGACTACTTCAAGTTGATGGGTAAAGCCAAGTTTTGCTTGTGCCCTAGTGGGTACGAAGTGGCAAGTCCAAGAGTAGTCACAGCAATTTCTATGGGGTGCGTCCCCGTCCTCATTTCTGACAACTATTCATTGCCCTTTAGCGACGTTCTTGACTGGAGCAAATTCTCGGTGTACATTCCGTCGAAAAAAATACCCGAAATCAAGACGATCTTGAAAGAGATTTCGCACTCGAGGTATTTGGAACTGCAAAAGAGTGTGACGGAGGTGCACCGGCATTTTACGGTCCATCGTCCTGCTCAGCCTTACGATATGCTTAACATGGTGCTCCACTCTGTGTGGCTAAGAAGACTCGATGTTTCACTGCTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

503

Amino Acids

57.35

Weight (kDa)

8.09

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 170 - 452 7.3e-58 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20260 AT5G20260 AT5G20260
malus_domestica MD02G1022700.v1.1 MD15G1165400.v1.1
prunus_persica Prupe.7G249400_v2.0.a1
pyrus_communis pycom15g14810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 350
AccB1I GGYRCC 1 cut(s) 847
AciI CCGC 2 cut(s) 230, 995
AclWI GGATC 2 cut(s) 466, 1054
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 1 cut(s) 1298
AcyI GRCGYC 1 cut(s) 945
AdeI CACNNNGTG 1 cut(s) 1471
AfaI GTAC 5 cut(s) 399, 710, 1121, 1190, 1310
AfiI CCNNNNNNNGG 3 cut(s) 143, 637, 1015
AgsI TTSAA 8 cut(s) 57, 356, 520, 574, 746, 1023, 1147, 1351
AjuI GAANNNNNNNTTGG 2 cut(s) 104, 136
AluBI AGCT 2 cut(s) 779, 866
AluI AGCT 2 cut(s) 779, 866
Alw21I GWGCWC 2 cut(s) 1407, 1464
Alw26I GTCTC 4 cut(s) 132, 231, 936, 1061
Alw44I GTGCAC 1 cut(s) 1403
AlwI GGATC 2 cut(s) 466, 1054
Ama87I CYCGRG 1 cut(s) 1366
AoxI GGCC 3 cut(s) 366, 566, 713
ApaI GGGCCC 1 cut(s) 570
ApaLI GTGCAC 1 cut(s) 1403
ApoI RAATTY 1 cut(s) 1298
AspLEI GCGC 1 cut(s) 321
AspS9I GGNCC 4 cut(s) 566, 567, 986, 1420
AsuC2I CCSGG 1 cut(s) 565
AsuHPI GGTGA 3 cut(s) 177, 198, 207
AvaI CYCGRG 1 cut(s) 1366
AvaII GGWCC 2 cut(s) 986, 1420
BaeGI GKGCMC 4 cut(s) 570, 850, 1181, 1407
BalI TGGCCA 1 cut(s) 368
BanI GGYRCC 1 cut(s) 847
BanII GRGCYC 1 cut(s) 570
BbsI GAAGAC 3 cut(s) 438, 1143, 1489
Bbv12I GWGCWC 2 cut(s) 1407, 1464
BccI CCATC 7 cut(s) 208, 253, 499, 607, 654, 1147, 1431
BceAI ACGGC 1 cut(s) 306
BciVI GTATCC 1 cut(s) 697
BclI TGATCA 2 cut(s) 736, 817
BcnI CCSGG 1 cut(s) 565
BcoDI GTCTC 4 cut(s) 132, 231, 936, 1061
BfaI CTAG 2 cut(s) 1124, 1182
BfuAI ACCTGC 1 cut(s) 350
BfuI GTATCC 1 cut(s) 697
BglII AGATCT 1 cut(s) 1068
BlpI GCTNAGC 1 cut(s) 1434
Bme1390I CCNGG 1 cut(s) 565
Bme18I GGWCC 2 cut(s) 986, 1420
BmeT110I CYCGRG 1 cut(s) 1366
BmgT120I GGNCC 4 cut(s) 566, 567, 986, 1420
BmiI GGNNCC 2 cut(s) 568, 849
BmrFI CCNGG 1 cut(s) 565
BmsI GCATC 2 cut(s) 156, 556
BpiI GAAGAC 3 cut(s) 438, 1143, 1489
BpmI CTGGAG 3 cut(s) 409, 1097, 1312
Bpu1102I GCTNAGC 1 cut(s) 1434
BpuMI CCSGG 1 cut(s) 565
BsaHI GRCGYC 1 cut(s) 945
BsaI GGTCTC 1 cut(s) 1061
BsaJI CCNNGG 2 cut(s) 552, 808
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 637, 1015
Bse118I RCCGGY 2 cut(s) 630, 1407
Bse1I ACTGG 1 cut(s) 1295
Bse3DI GCAATG 2 cut(s) 913, 1265
BseDI CCNNGG 2 cut(s) 552, 808
BseGI GGATG 5 cut(s) 200, 447, 547, 646, 672
BseLI CCNNNNNNNGG 3 cut(s) 143, 637, 1015
BseMI GCAATG 2 cut(s) 913, 1265
BseMII CTCAG 1 cut(s) 1448
BseNI ACTGG 1 cut(s) 1295
BseSI GKGCMC 4 cut(s) 570, 850, 1181, 1407
BsgI GTGCAG 1 cut(s) 21
BshFI GGCC 3 cut(s) 368, 568, 715
BshNI GGYRCC 1 cut(s) 847
BsiHKAI GWGCWC 2 cut(s) 1407, 1464
BsiHKCI CYCGRG 1 cut(s) 1366
BsiSI CCGG 5 cut(s) 144, 564, 631, 1041, 1408
BslFI GGGAC 2 cut(s) 919, 1223
BslI CCNNNNNNNGG 3 cut(s) 143, 637, 1015
BsmAI GTCTC 4 cut(s) 132, 231, 936, 1061
BsmBI CGTCTC 3 cut(s) 132, 231, 936
BsmFI GGGAC 2 cut(s) 919, 1223
BsnI GGCC 3 cut(s) 368, 568, 715
Bso31I GGTCTC 1 cut(s) 1061
BsoBI CYCGRG 1 cut(s) 1366
Bsp120I GGGCCC 1 cut(s) 566
Bsp1286I GDGCHC 5 cut(s) 570, 850, 1181, 1407, 1464
Bsp1407I TGTACA 2 cut(s) 708, 1308
Bsp143I GATC 7 cut(s) 384, 458, 736, 817, 1046, 1068, 1344
Bsp1720I GCTNAGC 1 cut(s) 1434
BspACI CCGC 2 cut(s) 230, 995
BspANI GGCC 3 cut(s) 368, 568, 715
BspCNI CTCAG 1 cut(s) 1447
BspHI TCATGA 1 cut(s) 838
BspLI GGNNCC 2 cut(s) 568, 849
BspMI ACCTGC 1 cut(s) 350
BspPI GGATC 2 cut(s) 466, 1054
BspQI GCTCTTC 2 cut(s) 22, 620
BspT107I GGYRCC 1 cut(s) 847
BspTNI GGTCTC 1 cut(s) 1061
BsrDI GCAATG 2 cut(s) 913, 1265
BsrFI RCCGGY 2 cut(s) 630, 1407
BsrGI TGTACA 2 cut(s) 708, 1308
BsrI ACTGG 1 cut(s) 1295
BssAI RCCGGY 2 cut(s) 630, 1407
BssECI CCNNGG 2 cut(s) 552, 808
BssMI GATC 7 cut(s) 384, 458, 736, 817, 1046, 1068, 1344
BssNI GRCGYC 1 cut(s) 945
BssT1I CCWWGG 1 cut(s) 552
Bst4CI ACNGT 4 cut(s) 734, 812, 1055, 1420
Bst6I CTCTTC 2 cut(s) 22, 620
BstACI GRCGYC 1 cut(s) 945
BstAUI TGTACA 2 cut(s) 708, 1308
BstC8I GCNNGC 1 cut(s) 643
BstDEI CTNAG 2 cut(s) 1434, 1478
BstDSI CCRYGG 1 cut(s) 808
BstENI CCTNNNNNAGG 1 cut(s) 1013
BstF5I GGATG 5 cut(s) 200, 447, 547, 646, 672
BstHHI GCGC 1 cut(s) 321
BstKTI GATC 7 cut(s) 387, 461, 739, 820, 1049, 1071, 1347
BstMAI GTCTC 4 cut(s) 132, 231, 936, 1061
BstMBI GATC 7 cut(s) 384, 458, 736, 817, 1046, 1068, 1344
BstMWI GCNNNNNNNGC 4 cut(s) 365, 380, 627, 1037
BstSCI CCNGG 1 cut(s) 563
BstSLI GKGCMC 4 cut(s) 570, 850, 1181, 1407
BstV2I GAAGAC 3 cut(s) 438, 1143, 1489
BstX2I RGATCY 2 cut(s) 1046, 1068
BstYI RGATCY 2 cut(s) 1046, 1068
BsuI GTATCC 1 cut(s) 697
BsuRI GGCC 3 cut(s) 368, 568, 715
BtgI CCRYGG 1 cut(s) 808
BtsCI GGATG 5 cut(s) 200, 447, 547, 646, 672
BtsI GCAGTG 2 cut(s) 38, 1496
BtsIMutI CAGTG 3 cut(s) 38, 688, 1496
BveI ACCTGC 1 cut(s) 350
Cac8I GCNNGC 1 cut(s) 643
CciI TCATGA 1 cut(s) 838
CfoI GCGC 1 cut(s) 321
Cfr10I RCCGGY 2 cut(s) 630, 1407
Cfr13I GGNCC 4 cut(s) 566, 567, 986, 1420
CseI GACGC 3 cut(s) 405, 953, 1225
Csp6I GTAC 5 cut(s) 398, 709, 1120, 1189, 1309
CspCI CAANNNNNGTGG 2 cut(s) 222, 257
CviAII CATG 6 cut(s) 161, 401, 637, 839, 1115, 1456
CviQI GTAC 5 cut(s) 398, 709, 1120, 1189, 1309
DdeI CTNAG 2 cut(s) 1434, 1478
DpnI GATC 7 cut(s) 386, 460, 738, 819, 1048, 1070, 1346
DpnII GATC 7 cut(s) 384, 458, 736, 817, 1046, 1068, 1344
DraIII CACNNNGTG 1 cut(s) 1471
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 2 cut(s) 22, 620
EarI CTCTTC 2 cut(s) 22, 620
EciI GGCGGA 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 552
Eco147I AGGCCT 1 cut(s) 715
Eco24I GRGCYC 1 cut(s) 570
Eco31I GGTCTC 1 cut(s) 1061
Eco47I GGWCC 2 cut(s) 986, 1420
Eco88I CYCGRG 1 cut(s) 1366
EcoNI CCTNNNNNAGG 1 cut(s) 1013
EcoO109I RGGNCCY 1 cut(s) 567
EcoT14I CCWWGG 1 cut(s) 552
EcoT38I GRGCYC 1 cut(s) 570
ErhI CCWWGG 1 cut(s) 552
Esp3I CGTCTC 3 cut(s) 132, 231, 936
FaeI CATG 6 cut(s) 164, 404, 640, 842, 1118, 1459
FalI AAGNNNNNCTT 4 cut(s) 1157, 1189, 1331, 1363
FaqI GGGAC 2 cut(s) 919, 1223
FatI CATG 6 cut(s) 160, 400, 636, 838, 1114, 1455
FauI CCCGC 1 cut(s) 1002
FauNDI CATATG 1 cut(s) 973
FbaI TGATCA 2 cut(s) 736, 817
FokI GGATG 5 cut(s) 187, 434, 534, 633, 659
FriOI GRGCYC 1 cut(s) 570
FspBI CTAG 2 cut(s) 1124, 1182
GlaI GCGC 1 cut(s) 320
GsuI CTGGAG 3 cut(s) 409, 1097, 1312
HaeIII GGCC 3 cut(s) 368, 568, 715
HapII CCGG 5 cut(s) 144, 564, 631, 1041, 1408
HgaI GACGC 3 cut(s) 405, 953, 1225
HhaI GCGC 1 cut(s) 321
Hin1I GRCGYC 1 cut(s) 945
Hin1II CATG 6 cut(s) 164, 404, 640, 842, 1118, 1459
Hin6I GCGC 1 cut(s) 319
HinP1I GCGC 1 cut(s) 319
HinfI GANTC 3 cut(s) 269, 409, 1485
HpaII CCGG 5 cut(s) 144, 564, 631, 1041, 1408
HphI GGTGA 3 cut(s) 177, 198, 207
Hpy166II GTNNAC 4 cut(s) 532, 559, 1309, 1405
Hpy188I TCNGA 4 cut(s) 492, 922, 990, 1255
Hpy188III TCNNGA 8 cut(s) 388, 650, 839, 956, 1286, 1339, 1348, 1506
Hpy8I GTNNAC 4 cut(s) 532, 559, 1309, 1405
Hpy99I CGWCG 4 cut(s) 611, 656, 1283, 1321
HpyAV CCTTC 8 cut(s) 201, 292, 514, 533, 580, 917, 1042, 1081
HpyCH4III ACNGT 4 cut(s) 734, 812, 1055, 1420
HpyCH4IV ACGT 1 cut(s) 1281
HpyCH4V TGCA 8 cut(s) 38, 326, 476, 906, 1108, 1114, 1384, 1405
HpyF10VI GCNNNNNNNGC 4 cut(s) 365, 380, 627, 1037
HpyF3I CTNAG 2 cut(s) 1434, 1478
HpySE526I ACGT 1 cut(s) 1281
Hsp92I GRCGYC 1 cut(s) 945
Hsp92II CATG 6 cut(s) 164, 404, 640, 842, 1118, 1459
HspAI GCGC 1 cut(s) 319
Ksp22I TGATCA 2 cut(s) 736, 817
Kzo9I GATC 7 cut(s) 384, 458, 736, 817, 1046, 1068, 1344
LguI GCTCTTC 2 cut(s) 22, 620
LmnI GCTCC 3 cut(s) 1078, 1293, 1467
LweI GCATC 2 cut(s) 156, 556
MaeI CTAG 2 cut(s) 1124, 1182
MaeII ACGT 1 cut(s) 1281
MaeIII GTNAC 5 cut(s) 31, 494, 721, 1213, 1393
MalI GATC 7 cut(s) 386, 460, 738, 819, 1048, 1070, 1346
MboI GATC 7 cut(s) 384, 458, 736, 817, 1046, 1068, 1344
MboII GAAGA 7 cut(s) 9, 443, 449, 637, 1027, 1148, 1494
MflI RGATCY 2 cut(s) 1046, 1068
MhlI GDGCHC 5 cut(s) 570, 850, 1181, 1407, 1464
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 9 cut(s) 57, 153, 340, 351, 421, 462, 700, 1221, 1298
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 1 cut(s) 1479
MmeI TCCRAC 2 cut(s) 714, 1013
Mox20I TGGCCA 1 cut(s) 368
MscI TGGCCA 1 cut(s) 368
MseI TTAA 3 cut(s) 92, 930, 1452
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 5 cut(s) 144, 564, 631, 1041, 1408
MspR9I CCNGG 1 cut(s) 565
MwoI GCNNNNNNNGC 4 cut(s) 365, 380, 627, 1037
NciI CCSGG 1 cut(s) 565
NdeI CATATG 1 cut(s) 973
NdeII GATC 7 cut(s) 384, 458, 736, 817, 1046, 1068, 1344
NlaIII CATG 6 cut(s) 164, 404, 640, 842, 1118, 1459
NlaIV GGNNCC 2 cut(s) 568, 849
NmuCI GTSAC 3 cut(s) 721, 1213, 1393
PaeR7I CTCGAG 1 cut(s) 1366
PagI TCATGA 1 cut(s) 838
PceI AGGCCT 1 cut(s) 715
PciSI GCTCTTC 2 cut(s) 22, 620
PfeI GAWTC 2 cut(s) 269, 409
PleI GAGTC 1 cut(s) 1479
PpsI GAGTC 1 cut(s) 1479
PspN4I GGNNCC 2 cut(s) 568, 849
PspOMI GGGCCC 1 cut(s) 566
PspPI GGNCC 4 cut(s) 566, 567, 986, 1420
PspXI VCTCGAGB 1 cut(s) 1366
PsuI RGATCY 2 cut(s) 1046, 1068
RsaI GTAC 5 cut(s) 399, 710, 1121, 1190, 1310
RsaNI GTAC 5 cut(s) 398, 709, 1120, 1189, 1309
SapI GCTCTTC 2 cut(s) 22, 620
SaqAI TTAA 3 cut(s) 92, 930, 1452
Sau3AI GATC 7 cut(s) 384, 458, 736, 817, 1046, 1068, 1344
Sau96I GGNCC 4 cut(s) 566, 567, 986, 1420
SchI GAGTC 1 cut(s) 1479
ScrFI CCNGG 1 cut(s) 565
SduI GDGCHC 5 cut(s) 570, 850, 1181, 1407, 1464
SfaNI GCATC 2 cut(s) 156, 556
Sfr274I CTCGAG 1 cut(s) 1366
SinI GGWCC 2 cut(s) 986, 1420
SlaI CTCGAG 1 cut(s) 1366
SmlI CTYRAG 1 cut(s) 1366
SmoI CTYRAG 1 cut(s) 1366
Sse9I AATT 9 cut(s) 57, 153, 340, 351, 421, 462, 700, 1221, 1298
SseBI AGGCCT 1 cut(s) 715
SsiI CCGC 2 cut(s) 230, 995
SspI AATATT 1 cut(s) 115
SspMI CTAG 2 cut(s) 1124, 1182
StuI AGGCCT 1 cut(s) 715
StyD4I CCNGG 1 cut(s) 563
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 4 cut(s) 734, 812, 1055, 1420
TaiI ACGT 1 cut(s) 1284
TaqI TCGA 5 cut(s) 606, 1011, 1319, 1367, 1488
TasI AATT 9 cut(s) 57, 153, 340, 351, 421, 462, 700, 1221, 1298
TatI WGTACW 3 cut(s) 397, 708, 1308
TfiI GAWTC 2 cut(s) 269, 409
Tru1I TTAA 3 cut(s) 92, 930, 1452
Tru9I TTAA 3 cut(s) 92, 930, 1452
TscAI CASTG 3 cut(s) 45, 688, 1503
TseFI GTSAC 3 cut(s) 721, 1213, 1393
Tsp45I GTSAC 3 cut(s) 721, 1213, 1393
TspDTI ATGAA 8 cut(s) 171, 282, 417, 436, 476, 625, 880, 1254
TspGWI ACGGA 3 cut(s) 213, 1305, 1412
TspRI CASTG 3 cut(s) 45, 688, 1503
VneI GTGCAC 1 cut(s) 1403
VpaK11BI GGWCC 2 cut(s) 986, 1420
XagI CCTNNNNNAGG 1 cut(s) 1013
XapI RAATTY 1 cut(s) 1298
XhoI CTCGAG 1 cut(s) 1366
XspI CTAG 2 cut(s) 1124, 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.