MD15G1165400.v1.1

glycosyltransferase At5g20260

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0019559
Physical Location & Seq
Forward (+)
12443359 .. 12445778
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1165400.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGAGTAATTTGGAGAGAATTGAAGCAGATTTGGGTAGAGCAAGAGAGGCGATCCGGAAAGCAGTTCATGAAAAGAATCAAACGTCTAATTACAAGGGAAACGACGATGTGATCATCCCAAAAGGATCAGTTTACAGAAACGCACAATCTTTTCATCAGAGCTACGTAGAGATGGAGAAAAGATTGAAGGTGTGGGTTTACAGGGAAGGAGAGCATCCGCTGGTTCACACCGGGCCCTTGAACGACATCTACGCCATCGAGGGGCAGTTTATAGACGAGATGGAGAGTGGTAAGAGCCGCTTCATGGCTCGCCATCCCGACGAGGCACACCTATTCCTCATTCCTATCAGTGTAGCCAACATCATCAACACTCTTTACAAGCCTCTTGTCACGTATAACCGTGATCAACTTCAAAGGGTGGTTATGGATTATATTGGCGTTATAGCTGAAAAATACCCTCACTGGAATAGAAGCCGAGGTGCTGACCATTTCTTGGTTTCTTGTCATGATTGGGCACCAGACATTTCACAAGTGAATCCTAAGTTATATCGAGACTTCATTAGGGTTTTATGCAATGCCAATATATCAGAAGGGTTTAAGCCACAAAGAGACGTCTCCATTCCAGAAATCAACATACCGTATGGAAAATTTGGTCCAACCCCGCAAAATACAAGCCTCGACAAGCGTTCAATCCTTGCCTTCTTCGCCGGAGGATCTCACGGCCGCATAAGAAAGATCTTGCTTGAGCATTGGAAGGACAAGGACAATGAAGTGCAAGTGCACGAGTACCTAAAAAACAAGAAAGACTACTTCAAGTTAATGGGTAAAGCCAAGTTTTGCTTGTGCCCTAGTGGGTACGAAGTGGCAAGTGCAAGGGTGGTCACAGCAATTTCCGTGGGGTGCGTCCCCGTGCTCATTTCCGATGACTACGTATTGCCCTTTAGTGACGTTCTTGACTGGAGCAAATTCTCCGTTTACATTCCATCGAAAAAAATACCCGAAATCAAGACAATTTTAAGGGGGATTTCGCCCTCGAGGTATTTGGAACTGCAAAAGAGCGTGATGGAGGTGCACCGGCATTTTACGATCCATCGCCCTGCTCAACCTTACGATATGCTTCACATGGTGCTTCACTCGGTCTGGTTGAGAAGGCTCGATGTTTCGTTGCTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

392

Amino Acids

45.15

Weight (kDa)

9.03

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 58 - 341 7.6e-60 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20260 AT5G20260 AT5G20260
malus_domestica MD02G1022700.v1.1 MD15G1165400.v1.1
prunus_persica Prupe.7G249400_v2.0.a1
pyrus_communis pycom15g14810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 615
AbsI CCTCGAGG 1 cut(s) 1033
AccB1I GGYRCC 1 cut(s) 514
AccB7I CCANNNNNTGG 1 cut(s) 493
AccIII TCCGGA 1 cut(s) 54
AciI CCGC 4 cut(s) 218, 298, 662, 724
AclWI GGATC 4 cut(s) 46, 133, 721, 1081
AcoI YGGCCR 1 cut(s) 721
AcsI RAATTY 2 cut(s) 647, 965
AcyI GRCGYC 1 cut(s) 612
AdeI CACNNNGTG 1 cut(s) 1126
AfaI GTAC 2 cut(s) 788, 857
AfiI CCNNNNNNNGG 3 cut(s) 261, 304, 493
AgsI TTSAA 6 cut(s) 23, 187, 241, 413, 690, 814
AluBI AGCT 2 cut(s) 162, 446
AluI AGCT 2 cut(s) 162, 446
Alw21I GWGCWC 3 cut(s) 783, 915, 1074
Alw26I GTCTC 3 cut(s) 546, 603, 619
Alw44I GTGCAC 2 cut(s) 779, 1070
AlwI GGATC 4 cut(s) 46, 133, 721, 1081
Ama87I CYCGRG 1 cut(s) 1033
Aor13HI TCCGGA 1 cut(s) 54
AoxI GGCC 2 cut(s) 233, 721
ApaI GGGCCC 1 cut(s) 237
ApaLI GTGCAC 2 cut(s) 779, 1070
ApoI RAATTY 2 cut(s) 647, 965
AspS9I GGNCC 3 cut(s) 233, 234, 653
AsuC2I CCSGG 1 cut(s) 232
AvaI CYCGRG 1 cut(s) 1033
AvaII GGWCC 1 cut(s) 653
BaeGI GKGCMC 5 cut(s) 237, 517, 783, 848, 1074
BanI GGYRCC 1 cut(s) 514
BanII GRGCYC 1 cut(s) 237
BauI CACGAG 1 cut(s) 782
Bbv12I GWGCWC 3 cut(s) 783, 915, 1074
BccI CCATC 7 cut(s) 166, 263, 274, 321, 991, 1057, 1098
BceAI ACGGC 1 cut(s) 736
BclI TGATCA 2 cut(s) 111, 403
BcnI CCSGG 1 cut(s) 232
BcoDI GTCTC 3 cut(s) 546, 603, 619
BfaI CTAG 1 cut(s) 849
BglII AGATCT 1 cut(s) 735
BisI GCNGC 2 cut(s) 298, 724
BlsI GCNGC 2 cut(s) 299, 725
Bme1390I CCNGG 1 cut(s) 232
Bme18I GGWCC 1 cut(s) 653
BmeT110I CYCGRG 1 cut(s) 1033
BmgT120I GGNCC 3 cut(s) 233, 234, 653
BmiI GGNNCC 2 cut(s) 235, 516
BmrFI CCNGG 1 cut(s) 232
BmsI GCATC 1 cut(s) 223
BpmI CTGGAG 1 cut(s) 979
BpuEI CTTGAG 1 cut(s) 764
BpuMI CCSGG 1 cut(s) 232
BsaAI YACGTR 3 cut(s) 166, 393, 931
BsaHI GRCGYC 1 cut(s) 612
BsaJI CCNNGG 2 cut(s) 475, 894
BsaWI WCCGGW 1 cut(s) 54
Bsc4I CCNNNNNNNGG 3 cut(s) 261, 304, 493
Bse118I RCCGGY 1 cut(s) 1074
Bse1I ACTGG 2 cut(s) 467, 962
Bse3DI GCAATG 1 cut(s) 580
BseAI TCCGGA 1 cut(s) 54
BseDI CCNNGG 2 cut(s) 475, 894
BseGI GGATG 3 cut(s) 114, 214, 313
BseLI CCNNNNNNNGG 3 cut(s) 261, 304, 493
BseMI GCAATG 1 cut(s) 580
BseNI ACTGG 2 cut(s) 467, 962
BseSI GKGCMC 5 cut(s) 237, 517, 783, 848, 1074
BseX3I CGGCCG 1 cut(s) 721
Bsh1285I CGRYCG 1 cut(s) 724
BshFI GGCC 2 cut(s) 235, 723
BshNI GGYRCC 1 cut(s) 514
BsiEI CGRYCG 1 cut(s) 724
BsiHKAI GWGCWC 3 cut(s) 783, 915, 1074
BsiHKCI CYCGRG 1 cut(s) 1033
BsiSI CCGG 4 cut(s) 55, 231, 708, 1075
BslFI GGGAC 1 cut(s) 890
BslI CCNNNNNNNGG 3 cut(s) 261, 304, 493
BsmAI GTCTC 3 cut(s) 546, 603, 619
BsmBI CGTCTC 2 cut(s) 603, 619
BsmFI GGGAC 1 cut(s) 890
BsnI GGCC 2 cut(s) 235, 723
BsoBI CYCGRG 1 cut(s) 1033
Bsp120I GGGCCC 1 cut(s) 233
Bsp1286I GDGCHC 6 cut(s) 237, 517, 783, 848, 915, 1074
Bsp13I TCCGGA 1 cut(s) 54
Bsp143I GATC 7 cut(s) 51, 111, 125, 403, 713, 735, 1086
BspACI CCGC 4 cut(s) 218, 298, 662, 724
BspANI GGCC 2 cut(s) 235, 723
BspEI TCCGGA 1 cut(s) 54
BspHI TCATGA 2 cut(s) 67, 505
BspLI GGNNCC 2 cut(s) 235, 516
BspPI GGATC 4 cut(s) 46, 133, 721, 1081
BspT107I GGYRCC 1 cut(s) 514
BsrDI GCAATG 1 cut(s) 580
BsrFI RCCGGY 1 cut(s) 1074
BsrI ACTGG 2 cut(s) 467, 962
BssAI RCCGGY 1 cut(s) 1074
BssECI CCNNGG 2 cut(s) 475, 894
BssMI GATC 7 cut(s) 51, 111, 125, 403, 713, 735, 1086
BssNI GRCGYC 1 cut(s) 612
BssSI CACGAG 1 cut(s) 782
Bst2BI CACGAG 1 cut(s) 782
Bst4CI ACNGT 2 cut(s) 401, 639
BstACI GRCGYC 1 cut(s) 612
BstBAI YACGTR 3 cut(s) 166, 393, 931
BstC8I GCNNGC 1 cut(s) 310
BstDEI CTNAG 1 cut(s) 540
BstDSI CCRYGG 1 cut(s) 894
BstF5I GGATG 3 cut(s) 114, 214, 313
BstKTI GATC 7 cut(s) 54, 114, 128, 406, 716, 738, 1089
BstMAI GTCTC 3 cut(s) 546, 603, 619
BstMBI GATC 7 cut(s) 51, 111, 125, 403, 713, 735, 1086
BstMCI CGRYCG 1 cut(s) 724
BstMWI GCNNNNNNNGC 2 cut(s) 47, 704
BstSCI CCNGG 1 cut(s) 230
BstSLI GKGCMC 5 cut(s) 237, 517, 783, 848, 1074
BstSNI TACGTA 2 cut(s) 166, 931
BstX2I RGATCY 2 cut(s) 713, 735
BstYI RGATCY 2 cut(s) 713, 735
BstZI CGGCCG 1 cut(s) 721
BsuRI GGCC 2 cut(s) 235, 723
BtgI CCRYGG 1 cut(s) 894
BtgZI GCGATG 1 cut(s) 1076
BtsCI GGATG 3 cut(s) 114, 214, 313
BtsIMutI CAGTG 2 cut(s) 355, 460
Cac8I GCNNGC 1 cut(s) 310
CciI TCATGA 2 cut(s) 67, 505
Cfr10I RCCGGY 1 cut(s) 1074
Cfr13I GGNCC 3 cut(s) 233, 234, 653
CseI GACGC 1 cut(s) 892
Csp6I GTAC 2 cut(s) 787, 856
CspCI CAANNNNNGTGG 2 cut(s) 876, 911
CviAII CATG 4 cut(s) 68, 304, 506, 1123
CviQI GTAC 2 cut(s) 787, 856
DdeI CTNAG 1 cut(s) 540
DpnI GATC 7 cut(s) 53, 113, 127, 405, 715, 737, 1088
DpnII GATC 7 cut(s) 51, 111, 125, 403, 713, 735, 1086
DraIII CACNNNGTG 1 cut(s) 1126
EaeI YGGCCR 1 cut(s) 721
EagI CGGCCG 1 cut(s) 721
EclXI CGGCCG 1 cut(s) 721
Eco105I TACGTA 2 cut(s) 166, 931
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 653
Eco52I CGGCCG 1 cut(s) 721
Eco88I CYCGRG 1 cut(s) 1033
EcoO109I RGGNCCY 1 cut(s) 234
EcoT38I GRGCYC 1 cut(s) 237
Esp3I CGTCTC 2 cut(s) 603, 619
FaeI CATG 4 cut(s) 71, 307, 509, 1126
FalI AAGNNNNNCTT 4 cut(s) 284, 316, 824, 856
FaqI GGGAC 1 cut(s) 890
FatI CATG 4 cut(s) 67, 303, 505, 1122
FauI CCCGC 1 cut(s) 669
FbaI TGATCA 2 cut(s) 111, 403
Fnu4HI GCNGC 2 cut(s) 298, 724
FokI GGATG 3 cut(s) 101, 201, 300
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 2 cut(s) 298, 724
FspBI CTAG 1 cut(s) 849
GluI GCNGC 2 cut(s) 298, 724
GsuI CTGGAG 1 cut(s) 979
HaeIII GGCC 2 cut(s) 235, 723
HapII CCGG 4 cut(s) 55, 231, 708, 1075
HgaI GACGC 1 cut(s) 892
Hin1I GRCGYC 1 cut(s) 612
Hin1II CATG 4 cut(s) 71, 307, 509, 1126
HinfI GANTC 2 cut(s) 76, 535
HpaII CCGG 4 cut(s) 55, 231, 708, 1075
Hpy166II GTNNAC 6 cut(s) 133, 199, 226, 781, 976, 1072
Hpy188I TCNGA 3 cut(s) 159, 589, 922
Hpy188III TCNNGA 9 cut(s) 55, 68, 317, 506, 551, 623, 953, 1006, 1173
Hpy8I GTNNAC 6 cut(s) 133, 199, 226, 781, 976, 1072
Hpy99I CGWCG 2 cut(s) 107, 323
HpyAV CCTTC 6 cut(s) 181, 200, 584, 709, 748, 1143
HpyCH4III ACNGT 2 cut(s) 401, 639
HpyCH4IV ACGT 6 cut(s) 83, 165, 392, 612, 930, 948
HpyCH4V TGCA 6 cut(s) 573, 775, 781, 872, 1051, 1072
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 704
HpyF3I CTNAG 1 cut(s) 540
HpySE526I ACGT 6 cut(s) 83, 165, 392, 612, 930, 948
Hsp92I GRCGYC 1 cut(s) 612
Hsp92II CATG 4 cut(s) 71, 307, 509, 1126
Kpn2I TCCGGA 1 cut(s) 54
Ksp22I TGATCA 2 cut(s) 111, 403
Kzo9I GATC 7 cut(s) 51, 111, 125, 403, 713, 735, 1086
LmnI GCTCC 1 cut(s) 960
LweI GCATC 1 cut(s) 223
MaeI CTAG 1 cut(s) 849
MaeII ACGT 6 cut(s) 83, 165, 392, 612, 930, 948
MaeIII GTNAC 3 cut(s) 388, 880, 944
MalI GATC 7 cut(s) 53, 113, 127, 405, 715, 737, 1088
MboI GATC 7 cut(s) 51, 111, 125, 403, 713, 735, 1086
MboII GAAGA 1 cut(s) 694
MflI RGATCY 2 cut(s) 713, 735
MhlI GDGCHC 6 cut(s) 237, 517, 783, 848, 915, 1074
MluCI AATT 7 cut(s) 7, 18, 88, 647, 888, 965, 1011
MmeI TCCRAC 1 cut(s) 680
MroI TCCGGA 1 cut(s) 54
MseI TTAA 3 cut(s) 597, 818, 1016
MspA1I CMGCKG 1 cut(s) 220
MspI CCGG 4 cut(s) 55, 231, 708, 1075
MspR9I CCNGG 1 cut(s) 232
MwoI GCNNNNNNNGC 2 cut(s) 47, 704
NciI CCSGG 1 cut(s) 232
NdeII GATC 7 cut(s) 51, 111, 125, 403, 713, 735, 1086
NlaIII CATG 4 cut(s) 71, 307, 509, 1126
NlaIV GGNNCC 2 cut(s) 235, 516
NmeAIII GCCGAG 1 cut(s) 500
NmuCI GTSAC 3 cut(s) 388, 880, 944
PaeR7I CTCGAG 1 cut(s) 1033
PagI TCATGA 2 cut(s) 67, 505
PfeI GAWTC 2 cut(s) 76, 535
PflMI CCANNNNNTGG 1 cut(s) 493
PkrI GCNGC 2 cut(s) 299, 725
Ppu21I YACGTR 3 cut(s) 166, 393, 931
PspN4I GGNNCC 2 cut(s) 235, 516
PspOMI GGGCCC 1 cut(s) 233
PspPI GGNCC 3 cut(s) 233, 234, 653
PspXI VCTCGAGB 1 cut(s) 1033
PsrI GAACNNNNNNTAC 2 cut(s) 233, 265
PsuI RGATCY 2 cut(s) 713, 735
RsaI GTAC 2 cut(s) 788, 857
RsaNI GTAC 2 cut(s) 787, 856
SaqAI TTAA 3 cut(s) 597, 818, 1016
SatI GCNGC 2 cut(s) 298, 724
Sau3AI GATC 7 cut(s) 51, 111, 125, 403, 713, 735, 1086
Sau96I GGNCC 3 cut(s) 233, 234, 653
ScrFI CCNGG 1 cut(s) 232
SduI GDGCHC 6 cut(s) 237, 517, 783, 848, 915, 1074
SfaNI GCATC 1 cut(s) 223
Sfr274I CTCGAG 1 cut(s) 1033
SinI GGWCC 1 cut(s) 653
SlaI CTCGAG 1 cut(s) 1033
SmlI CTYRAG 2 cut(s) 743, 1033
SmoI CTYRAG 2 cut(s) 743, 1033
SnaBI TACGTA 2 cut(s) 166, 931
Sse9I AATT 7 cut(s) 7, 18, 88, 647, 888, 965, 1011
SsiI CCGC 4 cut(s) 218, 298, 662, 724
SspMI CTAG 1 cut(s) 849
StyD4I CCNGG 1 cut(s) 230
TaaI ACNGT 2 cut(s) 401, 639
TaiI ACGT 6 cut(s) 86, 168, 395, 615, 933, 951
TaqI TCGA 6 cut(s) 258, 550, 678, 986, 1034, 1155
TaqII GACCGA 1 cut(s) 1126
TasI AATT 7 cut(s) 7, 18, 88, 647, 888, 965, 1011
TauI GCSGC 2 cut(s) 300, 726
TfiI GAWTC 2 cut(s) 76, 535
Tru1I TTAA 3 cut(s) 597, 818, 1016
Tru9I TTAA 3 cut(s) 597, 818, 1016
TscAI CASTG 2 cut(s) 355, 467
TseFI GTSAC 3 cut(s) 388, 880, 944
Tsp45I GTSAC 3 cut(s) 388, 880, 944
TspDTI ATGAA 6 cut(s) 56, 84, 143, 292, 547, 783
TspGWI ACGGA 2 cut(s) 883, 961
TspRI CASTG 2 cut(s) 355, 467
Van91I CCANNNNNTGG 1 cut(s) 493
VneI GTGCAC 2 cut(s) 779, 1070
VpaK11BI GGWCC 1 cut(s) 653
XapI RAATTY 2 cut(s) 647, 965
XhoI CTCGAG 1 cut(s) 1033
XspI CTAG 1 cut(s) 849
ZraI GACGTC 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.