AT5G23400

Leucine rich repeat N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014487
Physical Location & Seq
Forward (+)
7880272 .. 7882634
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23400.1

Sequence Viewer

Length: 1770 bp
ATGCAAAACCTGAAATGGGTTTTGATGAATCTCTTGTTTGTTTCTGCTCTTGTTCGTAATTTTGTTCTTTCATCATCACAACAAGTAATCTGTTCAAGCCAAGACAGAGCAACACTTCTGGGTTTCAAGTCAAGCATCATCGAGGACACAACAGGAGTTCTTGATTCATGGGTTGGTAAAGATTGTTGCAATGGAGATTGGGAAGGTGTTCAATGCAATCCAGCCACCGGAAAAGTCACCGGCTTGGTGTTACAATCCGCCGTGAACGAGCCTACTCTCTACATGAAAGGCACATTATCACCTTCTTTGGGTAATCTCCGATCTCTTGAGCTTTTGCTGATTACTGGAAACAAATTCATCACTGGTTCAATCCCCAACAGCTTCTCTAATCTGACCAGTCTTCGTCAGCTCATACTCGATGATAATTCTCTCCAAGGCAATGTTCTTTCTTCTTTAGGCCATCTTCCATTGTTGGAGATTCTCTCTTTAGCTGGGAATCGCTTCTCAGGTCTTGTCCCGGCGAGTTTTGGAAGCTTGAGACGTCTTACTACGATGAATTTGGCTCGAAACTCTTTCTCTGGTCCAATCCCAGTGACTTTCAAGAACCTCCTCAAACTTGAGAATCTTGATCTCAGCTCCAATTTGTTATCTGGACCAATCCCAGATTTCATAGGCCAGTTTCAGAATTTAACCAATCTTTATCTCTCCAGCAACAGATTCTCCGGGGTGTTACCAGTTTCTGTTTACAGCTTAAGGAAGCTTCAAACCATGTCGTTGGAGCGTAATGGTCTAACCGGACCACTCTCAGATCGATTCAGCTATTTGAAGTCACTCACTAGCCTTCAGCTAAGCGGCAACAAGTTCATCGGTCACATTCCAGCATCCATTACAGGACTGCAGAATCTTTGGTCTCTCAACCTCTCAAGAAACCTGTTCTCTGATCCTCTGCCTGTTGTTGGAGCCAGAGGGTTCCCTTCTCTACTGTCCATTGATCTTTCCTACAACAACCTTAATCTTGGAGCAATTCCAAGCTGGATCAGAGACAAGCAACTCTCAGATATCAACTTAGCTGGTTGCAAACTGAGAGGAACCTTTCCAAAGCTAACAAGACCAACCACCTTGACGTCGCTAGATTTGTCTGACAACTTTCTCACAGGTGATGTTTCAGCTTTCCTTACAAGTTTGACCAATGTTCAGAAAGTGAAGCTCTCAAAGAACCAGCTCAGGTTCGATCTCTCCAAGCTGAAGTTGCCAGAAGGAGTCGCGTCTATTGATCTGAGTTCAAATCTAGTGACCGGGTCCCTTTCAAGCCTGATAAACAACAAGACAAGCAGTTTCTTGGAAGAGATCCATCTCACCAACAACCAAATCTCAGGGAGGATTCCAGATTTCGGAGAAAGCTTGAACCTGAAAGTTCTCAACATAGGAAGCAACAAAATCAGTGGACAAATCCCAAGTTCAATATCAAACCTTGTTGAACTTGTGAGACTAGACATCTCAAGGAATCACATAACAGGAGGCATACCTCAAGCTATAGGACAGCTAGCACAGCTCAAATGGCTTGATCTCTCGATCAATGCACTTACAGGAAGGATCCCGGATAGCTTATTGAACATCAAGACGATCAAACATGCGAGTTTCAGAGCCAACAGATTGTGTGGACAGATTCCACAAGGAAGACCATTCAACATCTTTCCTGCAGCTGCTTATCTGCATAACCTTTGCTTGTGTGGTAAGCCATTACCAGCTTGTAGGAAGACAATGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

589

Amino Acids

64.02

Weight (kDa)

9.65

Isoelectric Point (pI)

35.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 73 3.4e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 99 - 308 2e-10 Leucine-rich repeat region
LRR_8 PF13855 157 - 216 7.2e-09 Leucine rich repeat
LRR_8 PF13855 229 - 288 1.4e-09 Leucine rich repeat
LRR_8 PF13855 447 - 504 1.3e-06 Leucine rich repeat
LRR_14 PF23598 461 - 542 4.9e-06 Leucine-rich repeat region
LRR_8 PF13855 469 - 528 1.8e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23400
fragaria_vesca FvH4_4g37290
malus_domestica MD13G1001800.v1.1
prunus_persica Prupe.1G350800_v2.0.a1
pyrus_communis pycom13g00140 pycom16g00040
rosa_chinensis RchiOBHm_Chr4g0447111
rosa_laevigata RLG00000005604
rosa_multiflora Rmu_sc0010825.1_g000008 Rmu_ssc0000178.1_g000011
rosa_roxburghii Rroxscaffold_5G00387450
rosa_rugosa Rorug04G0378300
rosa_samantha Rh4AG439400 Rh4BG448500 Rh4CG465500 Rh4DG446900
rosa_wichuraiana Rw4G037590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 544, 1127
AccII CGCG 1 cut(s) 1265
AciI CCGC 2 cut(s) 258, 852
AclWI GGATC 5 cut(s) 935, 1043, 1342, 1588, 1601
AcsI RAATTY 3 cut(s) 353, 556, 685
AcuI CTGAAG 2 cut(s) 827, 1265
AcyI GRCGYC 2 cut(s) 541, 1124
AfiI CCNNNNNNNGG 5 cut(s) 16, 227, 308, 956, 1391
AflII CTTAAG 1 cut(s) 751
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
Alw26I GTCTC 4 cut(s) 532, 915, 1035, 1480
AlwI GGATC 5 cut(s) 935, 1043, 1342, 1588, 1601
AlwNI CAGNNNCTG 1 cut(s) 740
AoxI GGCC 2 cut(s) 457, 673
ApeKI GCWGC 2 cut(s) 1700, 1703
ApoI RAATTY 3 cut(s) 353, 556, 685
Asp700I GAANNNNTTC 2 cut(s) 207, 500
AspS9I GGNCC 4 cut(s) 581, 653, 797, 1299
AsuC2I CCSGG 4 cut(s) 518, 724, 1297, 1598
AsuHPI GGTGA 4 cut(s) 229, 291, 1169, 1348
AsuNHI GCTAGC 1 cut(s) 1543
AvaII GGWCC 4 cut(s) 581, 653, 797, 1299
BamHI GGATCC 1 cut(s) 1593
BbsI GAAGAC 3 cut(s) 392, 1684, 1763
BbvI GCAGC 2 cut(s) 1690, 1712
BccI CCATC 2 cut(s) 468, 1359
BceAI ACGGC 1 cut(s) 245
BcnI CCSGG 4 cut(s) 518, 724, 1297, 1598
BcoDI GTCTC 4 cut(s) 532, 915, 1035, 1480
BfaI CTAG 5 cut(s) 837, 1130, 1289, 1490, 1544
BfmI CTRYAG 3 cut(s) 896, 1533, 1698
BfrI CTTAAG 1 cut(s) 751
BisI GCNGC 3 cut(s) 853, 1701, 1704
BlpI GCTNAGC 1 cut(s) 848
BlsI GCNGC 3 cut(s) 854, 1702, 1705
Bme1390I CCNGG 4 cut(s) 518, 724, 1297, 1598
Bme18I GGWCC 4 cut(s) 581, 653, 797, 1299
BmgT120I GGNCC 4 cut(s) 581, 653, 797, 1299
BmiI GGNNCC 6 cut(s) 961, 971, 1090, 1300, 1301, 1595
BmrFI CCNGG 4 cut(s) 518, 724, 1297, 1598
BmrI ACTGGG 1 cut(s) 584
BmsI GCATC 2 cut(s) 144, 890
BmtI GCTAGC 1 cut(s) 1547
BmuI ACTGGG 1 cut(s) 584
BpiI GAAGAC 3 cut(s) 392, 1684, 1763
BplI GAGNNNNNCTC 2 cut(s) 467, 499
BpmI CTGGAG 1 cut(s) 691
Bpu10I CCTNAGC 1 cut(s) 1223
Bpu1102I GCTNAGC 1 cut(s) 848
BpuEI CTTGAG 6 cut(s) 347, 556, 638, 907, 1483, 1512
BpuMI CCSGG 4 cut(s) 518, 724, 1297, 1598
Bsa29I ATCGAT 1 cut(s) 811
BsaHI GRCGYC 2 cut(s) 541, 1124
BsaI GGTCTC 1 cut(s) 915
BsaJI CCNNGG 2 cut(s) 433, 723
BsaWI WCCGGW 2 cut(s) 227, 794
BsaXI ACNNNNNCTCC 2 cut(s) 704, 734
Bsc4I CCNNNNNNNGG 5 cut(s) 16, 227, 308, 956, 1391
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 6 cut(s) 349, 367, 396, 590, 676, 734
Bse3DI GCAATG 2 cut(s) 196, 445
BseCI ATCGAT 1 cut(s) 811
BseDI CCNNGG 2 cut(s) 433, 723
BseGI GGATG 1 cut(s) 881
BseLI CCNNNNNNNGG 5 cut(s) 16, 227, 308, 956, 1391
BseMI GCAATG 2 cut(s) 196, 445
BseMII CTCAG 8 cut(s) 519, 646, 819, 1068, 1073, 1237, 1268, 1386
BseNI ACTGG 6 cut(s) 349, 367, 396, 590, 676, 734
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 2 cut(s) 1690, 1712
BseYI CCCAGC 1 cut(s) 491
Bsh1236I CGCG 1 cut(s) 1265
BshFI GGCC 2 cut(s) 459, 675
BshVI ATCGAT 1 cut(s) 811
BsiSI CCGG 7 cut(s) 228, 240, 518, 723, 795, 1296, 1598
BslFI GGGAC 2 cut(s) 500, 1285
BslI CCNNNNNNNGG 5 cut(s) 16, 227, 308, 956, 1391
BsmAI GTCTC 4 cut(s) 532, 915, 1035, 1480
BsmBI CGTCTC 1 cut(s) 532
BsmFI GGGAC 2 cut(s) 500, 1285
BsnI GGCC 2 cut(s) 459, 675
Bso31I GGTCTC 1 cut(s) 915
Bsp1720I GCTNAGC 1 cut(s) 848
BspACI CCGC 2 cut(s) 258, 852
BspANI GGCC 2 cut(s) 459, 675
BspCNI CTCAG 8 cut(s) 518, 645, 818, 1067, 1074, 1236, 1269, 1385
BspDI ATCGAT 1 cut(s) 811
BspFNI CGCG 1 cut(s) 1265
BspLI GGNNCC 6 cut(s) 961, 971, 1090, 1300, 1301, 1595
BspMAI CTGCAG 2 cut(s) 900, 1702
BspOI GCTAGC 1 cut(s) 1547
BspPI GGATC 5 cut(s) 935, 1043, 1342, 1588, 1601
BspTI CTTAAG 1 cut(s) 751
BspTNI GGTCTC 1 cut(s) 915
BsrDI GCAATG 2 cut(s) 196, 445
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 6 cut(s) 349, 367, 396, 590, 676, 734
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 433, 723
BssNI GRCGYC 2 cut(s) 541, 1124
BssT1I CCWWGG 1 cut(s) 433
Bst4CI ACNGT 1 cut(s) 984
Bst6I CTCTTC 1 cut(s) 1338
BstACI GRCGYC 2 cut(s) 541, 1124
BstAFI CTTAAG 1 cut(s) 751
BstC8I GCNNGC 1 cut(s) 1545
BstF5I GGATG 1 cut(s) 881
BstFNI CGCG 1 cut(s) 1265
BstMAI GTCTC 4 cut(s) 532, 915, 1035, 1480
BstMWI GCNNNNNNNGC 3 cut(s) 1249, 1549, 1558
BstNSI RCATGY 1 cut(s) 1634
BstSCI CCNGG 4 cut(s) 516, 722, 1295, 1596
BstSFI CTRYAG 3 cut(s) 896, 1533, 1698
BstUI CGCG 1 cut(s) 1265
BstV1I GCAGC 2 cut(s) 1690, 1712
BstV2I GAAGAC 3 cut(s) 392, 1684, 1763
BstX2I RGATCY 2 cut(s) 1347, 1593
BstXI CCANNNNNNTGG 1 cut(s) 775
BstYI RGATCY 2 cut(s) 1347, 1593
Bsu15I ATCGAT 1 cut(s) 811
BsuRI GGCC 2 cut(s) 459, 675
BsuTUI ATCGAT 1 cut(s) 811
BtsCI GGATG 1 cut(s) 881
BtsIMutI CAGTG 3 cut(s) 360, 597, 1447
Cac8I GCNNGC 1 cut(s) 1545
CaiI CAGNNNCTG 1 cut(s) 740
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 4 cut(s) 581, 653, 797, 1299
ClaI ATCGAT 1 cut(s) 811
CseI GACGC 1 cut(s) 1254
CspCI CAANNNNNGTGG 2 cut(s) 1423, 1458
CviAII CATG 4 cut(s) 168, 283, 769, 1631
Eam1104I CTCTTC 1 cut(s) 1338
EarI CTCTTC 1 cut(s) 1338
EciI GGCGGA 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 915
Eco32I GATATC 1 cut(s) 1060
Eco47I GGWCC 4 cut(s) 581, 653, 797, 1299
Eco57I CTGAAG 2 cut(s) 827, 1265
EcoO109I RGGNCCY 1 cut(s) 1299
EcoRV GATATC 1 cut(s) 1060
EcoT14I CCWWGG 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 433
Esp3I CGTCTC 1 cut(s) 532
FaeI CATG 4 cut(s) 171, 286, 772, 1634
FaqI GGGAC 2 cut(s) 500, 1285
FatI CATG 4 cut(s) 167, 282, 768, 1630
Fnu4HI GCNGC 3 cut(s) 853, 1701, 1704
FokI GGATG 1 cut(s) 868
Fsp4HI GCNGC 3 cut(s) 853, 1701, 1704
FspBI CTAG 5 cut(s) 837, 1130, 1289, 1490, 1544
GluI GCNGC 3 cut(s) 853, 1701, 1704
GsaI CCCAGC 1 cut(s) 495
GsuI CTGGAG 1 cut(s) 691
HaeIII GGCC 2 cut(s) 459, 675
HapII CCGG 7 cut(s) 228, 240, 518, 723, 795, 1296, 1598
HgaI GACGC 1 cut(s) 1254
Hin1I GRCGYC 2 cut(s) 541, 1124
Hin1II CATG 4 cut(s) 171, 286, 772, 1634
HindIII AAGCTT 3 cut(s) 532, 758, 1399
HpaII CCGG 7 cut(s) 228, 240, 518, 723, 795, 1296, 1598
HphI GGTGA 4 cut(s) 229, 291, 1169, 1348
Hpy166II GTNNAC 4 cut(s) 265, 745, 1445, 1661
Hpy188III TCNNGA 9 cut(s) 161, 326, 601, 626, 651, 924, 1385, 1570, 1618
Hpy8I GTNNAC 4 cut(s) 265, 745, 1445, 1661
Hpy99I CGWCG 1 cut(s) 1129
HpyAV CCTTC 6 cut(s) 197, 312, 851, 984, 1250, 1584
HpyCH4III ACNGT 1 cut(s) 984
HpyCH4IV ACGT 2 cut(s) 541, 1124
HpyCH4V TGCA 8 cut(s) 4, 189, 216, 898, 1077, 1580, 1700, 1714
HpyF10VI GCNNNNNNNGC 3 cut(s) 1249, 1549, 1558
HpySE526I ACGT 2 cut(s) 541, 1124
Hsp92I GRCGYC 2 cut(s) 541, 1124
Hsp92II CATG 4 cut(s) 171, 286, 772, 1634
KflI GGGWCCC 1 cut(s) 1299
LmnI GCTCC 4 cut(s) 641, 778, 959, 1019
Lsp1109I GCAGC 2 cut(s) 1690, 1712
LweI GCATC 2 cut(s) 144, 890
MaeI CTAG 5 cut(s) 837, 1130, 1289, 1490, 1544
MaeII ACGT 2 cut(s) 541, 1124
MaeIII GTNAC 7 cut(s) 235, 249, 592, 729, 828, 869, 1291
MboII GAAGA 6 cut(s) 392, 441, 455, 1355, 1689, 1768
MflI RGATCY 2 cut(s) 1347, 1593
MluCI AATT 7 cut(s) 58, 353, 424, 556, 640, 685, 1023
MlyI GAGTC 1 cut(s) 1269
MmeI TCCRAC 3 cut(s) 453, 756, 937
MroXI GAANNNNTTC 2 cut(s) 207, 500
MseI TTAA 3 cut(s) 689, 752, 1011
MspA1I CMGCKG 1 cut(s) 1703
MspCI CTTAAG 1 cut(s) 751
MspI CCGG 7 cut(s) 228, 240, 518, 723, 795, 1296, 1598
MspR9I CCNGG 4 cut(s) 518, 724, 1297, 1598
MvnI CGCG 1 cut(s) 1265
MwoI GCNNNNNNNGC 3 cut(s) 1249, 1549, 1558
NciI CCSGG 4 cut(s) 518, 724, 1297, 1598
NheI GCTAGC 1 cut(s) 1543
NlaIII CATG 4 cut(s) 171, 286, 772, 1634
NlaIV GGNNCC 6 cut(s) 961, 971, 1090, 1300, 1301, 1595
NmuCI GTSAC 5 cut(s) 235, 592, 828, 869, 1291
NspI RCATGY 1 cut(s) 1634
PdmI GAANNNNTTC 2 cut(s) 207, 500
PflFI GACNNNGTC 1 cut(s) 1297
PfoI TCCNGGA 1 cut(s) 1596
PkrI GCNGC 3 cut(s) 854, 1702, 1705
PleI GAGTC 1 cut(s) 1268
PpsI GAGTC 1 cut(s) 1268
PpuMI RGGWCCY 1 cut(s) 1299
Psp5II RGGWCCY 1 cut(s) 1299
PspFI CCCAGC 1 cut(s) 491
PspN4I GGNNCC 6 cut(s) 961, 971, 1090, 1300, 1301, 1595
PspPI GGNCC 4 cut(s) 581, 653, 797, 1299
PspPPI RGGWCCY 1 cut(s) 1299
PstI CTGCAG 2 cut(s) 900, 1702
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 2 cut(s) 1347, 1593
PsyI GACNNNGTC 1 cut(s) 1297
PvuII CAGCTG 1 cut(s) 1703
SaqAI TTAA 3 cut(s) 689, 752, 1011
SatI GCNGC 3 cut(s) 853, 1701, 1704
Sau96I GGNCC 4 cut(s) 581, 653, 797, 1299
SchI GAGTC 1 cut(s) 1269
ScrFI CCNGG 4 cut(s) 518, 724, 1297, 1598
SfaNI GCATC 2 cut(s) 144, 890
SfcI CTRYAG 3 cut(s) 896, 1533, 1698
SinI GGWCC 4 cut(s) 581, 653, 797, 1299
SmlI CTYRAG 7 cut(s) 326, 535, 617, 751, 922, 1498, 1527
SmoI CTYRAG 7 cut(s) 326, 535, 617, 751, 922, 1498, 1527
Sse9I AATT 7 cut(s) 58, 353, 424, 556, 640, 685, 1023
SsiI CCGC 2 cut(s) 258, 852
SspMI CTAG 5 cut(s) 837, 1130, 1289, 1490, 1544
StyD4I CCNGG 4 cut(s) 516, 722, 1295, 1596
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 1 cut(s) 984
TaiI ACGT 2 cut(s) 544, 1127
TaqI TCGA 6 cut(s) 141, 417, 565, 811, 1230, 1571
TaqII GACCGA 1 cut(s) 857
TasI AATT 7 cut(s) 58, 353, 424, 556, 640, 685, 1023
TauI GCSGC 1 cut(s) 855
Tru1I TTAA 3 cut(s) 689, 752, 1011
Tru9I TTAA 3 cut(s) 689, 752, 1011
TscAI CASTG 3 cut(s) 367, 597, 1447
TseFI GTSAC 5 cut(s) 235, 592, 828, 869, 1291
TseI GCWGC 2 cut(s) 1700, 1703
Tsp45I GTSAC 5 cut(s) 235, 592, 828, 869, 1291
TspDTI ATGAA 8 cut(s) 41, 60, 156, 299, 346, 569, 658, 853
TspRI CASTG 3 cut(s) 367, 597, 1447
Tth111I GACNNNGTC 1 cut(s) 1297
Vha464I CTTAAG 1 cut(s) 751
VpaK11BI GGWCC 4 cut(s) 581, 653, 797, 1299
XapI RAATTY 3 cut(s) 353, 556, 685
XceI RCATGY 1 cut(s) 1634
XmnI GAANNNNTTC 2 cut(s) 207, 500
XspI CTAG 5 cut(s) 837, 1130, 1289, 1490, 1544
ZraI GACGTC 2 cut(s) 542, 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.