AT5G27970

C-terminal region of Mon2 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007304
Physical Location & Seq
Forward (+)
10004411 .. 10015746
11336 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27970.4

Sequence Viewer

Length: 4893 bp
ATGGCTTTGGTGGCGGCTTTAGAGGCGGATCTTCGCGCTCTCTCCGCCGAAGCGCGGCGGAGGTATCCCGCCGTGAAAGACGGAGCCGAGCACGCAATCCTAAAGCTTCGGTCGTCATCGAGTGCCAGTGATCTTTCAAGCAATGAAGACATTCTTCGGATATTTCTGATGGCGTGTGGAGTCAGGAATACAAAGCTTAGTGTTATAGGACTTTCCTGCTTGCAGAAGCTTATATCTCATGATGCAGTTGAACCATCATCTCTAAAGGAGATACTTTATACATTGAAAGATGCAAAACAGCTATCTGACGCCGTGTTTCCTTACTTGCAGCATTCAGAAATGGCAGAAGAAAATATTCAACTAAAGACTCTACAAACTATTCTAATAATATTTCAGTCTCGACTACATCCAGAAACAGAGGATAATATGGTCCTAGGACTAAGCATCTGTCTCACGCTTCTTGATAATAACCGTCCTCCTAGTGTTTACAATACTGCTGCAGCTACCTTCAGGCAAGCAGTTGCATTGATTTTTGATCAAGTGGTTTCGGCTGAGTCCCTTCCTATGCCTAAATTTGGTTCGAGCAGTCAAACAGCAAGGACTGGATCAGTTACTGGAGATTTGAGTCAGAATATCAATAATTCAGGGCCCTTGGAGAAGGATGTTATCGGTGGTCGATTGACAATAAGAGACACCCTGAGTGAAACCGGTAAACTTGGTCTGCGGTTGCTTGAGGACCTGACAGCCTCAGCTGCAGGTGGATCTGCAGCTTGGTTGCATGTTACTTCTCTTCCAAGGACGTTTTCCCTCGAACTAATTGAGTTTGTTTTGTCGAACTATATATCAGTTTTTAAGATACTACTTCCCTACGAACAGGTTTTGCGTCACCAAATCTGTTCCCTTCTGATGACATCGCTTCGGACCAGTTCAGAGCTCGAGGGAGAAATGGTTGAACCTTATTTTCGCCGGTTAGTGCTGCGGTCGGTTGCGCATATTATAAGACTGTATAGTTCGTCTCTCATCACAGAATGCGAGGTGTTCCTAAGTATGCTCGTGAAAGCTACATTTTTAGATTTGCCATTGTGGCATCGCATTCTTGTTCTTGAGATTTTGCGGGGGTTCTGTGTTGAGGCAAGAACATTACGGATTCTTTTCCAGAACTTTGATATGCACCCCAAAAACACGAATGTTGTGGAGAGCATGGTCAAAGCACTTGCTCGAGTTGTTTCTAGCATACAGTTTCAGGAGACGAGTGAGGAAAGTCTAGCAGCTGTTGCTGGGATGTTTAGTAGCAAAGCTAAAGGAATTGAGTGGATTCTTGATAATGATGCATCCAGTGCTGCTGTTCTCGTTGCGAGTGAAGCTCATGCAATAACGTTGGCAATTGAAGGCTTACTTGGAGTTGTTTTTACAGTTGCCACATTAACAGATGAAGCAGTAGATGTTGGGGAGCTTGAATCTCCCAGATACGAGCATCTTCCTTCATCAGATTATACTGGGAAAACCTCACTTCTCTGTATCTCAATGGTAGATTCATTGTGGTTGACTATACTTGACGCATTTTCCCTTATATTATCAAGGTCACAAGGAGAGGCAATCGTATTAGAGATACTGAAAGGATATCAAGCATTTACTCAGGCATGTGGCGTTCTCCATGCTGTAGAACCCTTGAACTCTTTTCTTGCATCGCTTTGTAAATTTACTATCGTTTTGCCAACTGATGTGGAAAGGAAAAGCGTCGTACAGTCGCCTGTGTCCAAACGTTCTGAAGTACAGGTTGATCTGAAAGATGTCATTGTCCTGACACCCAAGAATGTTCAGGCATTGAGAACACTCTTCAACATCGCTCACAGGTTGCATAACGTGTTGGGCCCATCATGGGTGTTGGTTCTTGAAACTCTGGCAGCCCTAGATCGAGCTATTCATTCTCCTCATGCAACTACCCAGGAGGTTGCAACTGCAGTCCCAAAGCTTACCAGAGAGCCTTCTAGGCAGTACGCTGATTTTAGCATTCTATCCTCCTTAAATTCTCAGCTGTTTGAGAGCTCAGCGCTGATGCAAGTGTCTTCTGTTAAATCACTTCTTTCTGCACTCCATATGTTGTCCCATCAATCCATGACAGAAACTTCAGGCAGTGTTTCATCAGCTTCAAGTAAACAAATTGGGAGCATCAGTTTTTCGGTGGACAGAATGATATCTATACTCGTAAATAATCTTCACAGGGTGGAACCACTATGGGACCAAGTGGTTGGCCACTTTCTTGAGCTCGCTGAACATTCAAATCAGAACTTGAGAAATATGGCGCTTGATGCCCTGGATCAATCCATATGTGCTGTCTTAGGTTCAGAGCAGTTTGGAGAAGATCCAGCCAGATCAAGAGATGCAACTTTGGATGTAGACTCAAAGTCAACTGAGGTGAAGTCAGTTGAGTGTGCTGTACTATCTTCTCTCAGGGTTCTTTACTTTTCTGCTCAAAAGGCTGATGTTCGAGTTGGGTCATTAAAAATTCTTCTTCACGTACTGGAGAGATGTGGAGAAAAGTTATACTATAGCTGGTCGAGCATTCTTGAAATGTTGAGGTCTGTTGCAGACGCATCAGAGAAGGATGTGGCGACCCTTGGGTTCCAGAGTCTCCGGGTTATTATGAGTGATGGACTTCCTACACTCCCGGAAGACTGTCTTCATGTGTGCATAGACGTTACTGGAGCTTATAGTGCACAAAAGACTGATTTGAATATAAGTTTGACTGCCATTGGCCTGTTGTGGACTTTGACTGATTTCGTAGCAAAAGGACTCCACCATGGGTCTCTAGTGGAGAAAGGATCGGGATTCAATAATGCAGATTCCACTCCACAGCAAACAAATGGCGAGGACGGGGAAAAGCATATGGGTAGCAATTCGGGCAAGTCAGATTATGAAGCTCCAATACAAGTGGTCAATCATGAGAAATTACTGTTCTTAGTCTTTTCGTTAATTCAGAAACTTGTTGATGACGAACGACCAGAGGTCAGGAATTCAGCTGTCAGGACATTTTTTCAGATTTTGGGAAGTCATGGAAACAAACTTTCAAAAAGCATGTGGGAGGATTGTCTATGGAACTATATCTTCCCAATGTTGGATGGCGCCTCTCACAAGGCTGCAACATCATCGAAGGACGAATGGCAAGGGAAAGAAATCGGTACTCGAGGAGGGAAAGCTGTGCACATGCTTATACATCATAGTCGCAATTCAGCCCAGAAGCAGTGGGATGAAACATTTGTACTTGTCCTTGGAGGAATAGCCCGCCTCTTCCGGTCCTACTTTCCTCTTCTTGAAAGCTTACCTAACTTCTGGTCAGGATGGGAGTCTTTGCTTGCTTTTGTTAAGAAAAGCATTTTCAATGGGAGTAAAGAGGTATCACTTGCAGCGATAAATTGTCTGCAGACAGCTGTTGTGTCCCACTGTGTCAAGGGAAACTTGCAACTTCGATATCTTAATTCGGTACTGGACGTGTATGAGCTTGTTTTCCAGAAGTCATCGAGTTACACAGGTGACACAGCAGCCAAGGTGAAACAAGAGATTCTGCATGGTTTAGGTGAATTATATGTGCAATCATCGAAGATGTTTGATGATAAAATGTACATGCAATTGTTGGGAATTGTTGATTTGGCTATAAAACAGGCCATCATAAATAGCGAAAATTTCGAAACTGAATATGGACATGTACCCCCTGTACTACGTCATGTTTTGGAAATCTTGCCCTCTTTGGGGCCTCCTGAACACCTTTCGTCAATGTGGCTAATCTTGCTTAGAGAGTTCTTGCATTATCTTCCACGGGTTGATTCTGTTCTGCCAAATGACGAAGGCAGTGAAGTATTGGAACAGAAAGCTGATGCTTCGTCTGAAACAATTCCAACCACAAGAATTACGACTAATATGTTTGCAGAAAAGCTGATCCCTGCCTTGATAGAGCTTCTCCTTCAGGCTCCTGCAGTTGAAAAGTATATCCTGTTTCCGGAAGTCATCCAGAACCTGAGAAGGTGCATGATGACAAGACGAGACAATCCAGATGGTTCACTCTGGAAGGTAGCAGCTGAGGGCTTCAACCGTTTACTTGTTGAAGATGTGAAGTTATGTTCTGTGGGTGGTGAAACAGAACTGAAAATTAGCAAAACAGCTCGAATACGCATTTGGAAAGAAATTGGAGATGTTTACGATATATTCCTTGTTGGTTATTGTGGACGTGCACTTTCTTCAAATTCTCTCCCTGCTGCAACACTTAAGGCCAATGAGACCCTTGAGATTGCCTTATTAAACGGTCTTGGCGATATTATTCTTAAGTCGACCGTGGATGCACCCCGAGAAGTCCTGGAGCGTCTTGTTTCAACTCTAGATCGATGTGCATCACGTACATGCTCATTGCCTGTTGAAACTGTAGAGCTGATGCCTGCCCATTGTAGCAGATTTTCCTTGACATGCTTACAAAAATTGTTTTCCTTGAGCAGCTCTGAAACCGAGAACTGGCACTCCACAAGAGCAGAAGTGAGCAAGATCTCTATCACCACACTAATGGCCAGATGTGAATTCATTTTGAGTAGATTTTTAATCGACGAAAATAATCTAGGCAACCGTCCAATCCCAACAGCTAGACTTGAAGAGATTATCTTTACCCTCCAAGAACTTTACCGCCTCAGCATTCATCCTGAGGTTGCATCTGTTCTTCCGTTGCAACCCTACCTGAAAAACGTTCTACGCGAAGATAACCGTGACACCCGTGCACACCTACTTGTCCTTTTTCCCTCTCTGTGCGAGATTGTGTTATCAAGGGAAATGCGGGTGAGAGAGCTGGTGCAAATTTTACTACGAGCAGTTGCTACGGAACTAGGCCTAGAAAAGGTTAGTCTATCCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7_N

Protein Analysis

1630

Amino Acids

180.48

Weight (kDa)

5.73

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 3 - 169 3.6e-37 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 244 - 384 2.6e-21 Mon2/Sec7/BIG1-like, HUS domain
Mon2_C PF16206 872 - 1132 6.3e-49 C-terminal region of Mon2 protein
Mon2_C PF16206 1294 - 1588 6.8e-10 C-terminal region of Mon2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 998
AarI CACCTGC 1 cut(s) 746
Acc16I TGCGCA 1 cut(s) 990
Acc36I ACCTGC 1 cut(s) 746
AccB1I GGYRCC 1 cut(s) 3122
AccI GTMKAC 2 cut(s) 2397, 4322
AccII CGCG 3 cut(s) 36, 55, 4734
AccIII TCCGGA 1 cut(s) 3994
AclI AACGTT 3 cut(s) 1376, 1762, 4725
AclWI GGATC 7 cut(s) 36, 613, 769, 2325, 2357, 2830, 3928
AcoI YGGCCR 2 cut(s) 2251, 4551
AcsI RAATTY 9 cut(s) 572, 1697, 2026, 2505, 3013, 3679, 4237, 4562, 4833
AcuI CTGAAG 4 cut(s) 493, 1788, 2112, 3944
AcyI GRCGYC 2 cut(s) 309, 3123
AdeI CACNNNGTG 1 cut(s) 2224
AfeI AGCGCT 1 cut(s) 2052
AfiI CCNNNNNNNGG 9 cut(s) 54, 575, 1879, 3115, 3746, 3747, 3994, 4500, 4873
AflII CTTAAG 2 cut(s) 4259, 4316
AflIII ACRYGT 3 cut(s) 1863, 3489, 3700
AgeI ACCGGT 1 cut(s) 707
AhdI GACNNNNNGTC 2 cut(s) 2404, 3005
AjiI CACGTC 2 cut(s) 3490, 4223
AjnI CCWGG 3 cut(s) 1944, 2313, 4347
AjuI GAANNNNNNNTTGG 6 cut(s) 787, 819, 1380, 1412, 4153, 4185
AloI GAACNNNNNNTCC 2 cut(s) 4490, 4522
Alw21I GWGCWC 8 cut(s) 93, 936, 2048, 2268, 2719, 3204, 4228, 4759
Alw26I GTCTC 9 cut(s) 402, 455, 684, 1020, 1241, 2636, 2811, 4032, 4265
Alw44I GTGCAC 4 cut(s) 2715, 3200, 4224, 4755
AlwI GGATC 7 cut(s) 36, 613, 769, 2325, 2357, 2830, 3928
AlwNI CAGNNNCTG 2 cut(s) 614, 4012
Ama87I CYCGRG 4 cut(s) 935, 1218, 3183, 4338
Aor13HI TCCGGA 1 cut(s) 3994
Aor51HI AGCGCT 1 cut(s) 2052
AoxI GGCC 9 cut(s) 647, 1870, 2251, 2755, 3660, 3749, 4263, 4551, 4864
ApaI GGGCCC 2 cut(s) 651, 1874
ApaLI GTGCAC 4 cut(s) 2715, 3200, 4224, 4755
ApoI RAATTY 9 cut(s) 572, 1697, 2026, 2505, 3013, 3679, 4237, 4562, 4833
AsiGI ACCGGT 1 cut(s) 707
Asp700I GAANNNNTTC 3 cut(s) 150, 354, 3888
AspA2I CCTAGG 1 cut(s) 433
AspLEI GCGC 6 cut(s) 38, 55, 991, 2053, 2305, 3125
AsuC2I CCSGG 2 cut(s) 2636, 2669
AsuHPI GGTGA 8 cut(s) 878, 2428, 3542, 3559, 3587, 4139, 4531, 4828
AsuII TTCGAA 1 cut(s) 3684
AvaI CYCGRG 4 cut(s) 935, 1218, 3183, 4338
AvaII GGWCC 5 cut(s) 430, 736, 921, 2239, 3294
AvrII CCTAGG 1 cut(s) 433
AxyI CCTNAGG 1 cut(s) 4683
BaeGI GKGCMC 6 cut(s) 651, 1874, 2719, 3204, 4228, 4759
BalI TGGCCA 2 cut(s) 2253, 4553
BanI GGYRCC 1 cut(s) 3122
BanII GRGCYC 5 cut(s) 651, 936, 1874, 2048, 2268
BarI GAAGNNNNNNTAC 4 cut(s) 2199, 2231, 2910, 2942
BauI CACGAG 1 cut(s) 1052
BbsI GAAGAC 4 cut(s) 153, 2058, 2672, 2679
Bbv12I GWGCWC 8 cut(s) 93, 936, 2048, 2268, 2719, 3204, 4228, 4759
BbvCI CCTCAGC 3 cut(s) 748, 4074, 4670
BccI CCATC 9 cut(s) 163, 262, 1882, 2115, 2645, 3113, 3333, 3671, 4043
BceAI ACGGC 2 cut(s) 56, 296
BcgI CGANNNNNNTGC 2 cut(s) 3129, 3163
BciT130I CCWGG 3 cut(s) 1946, 2315, 4349
BciVI GTATCC 1 cut(s) 75
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 2 cut(s) 2636, 2669
BcoDI GTCTC 9 cut(s) 402, 455, 684, 1020, 1241, 2636, 2811, 4032, 4265
BfmI CTRYAG 9 cut(s) 498, 753, 765, 1659, 1959, 2548, 3419, 3969, 4413
BfoI RGCGCY 3 cut(s) 2054, 2306, 3126
BfrI CTTAAG 2 cut(s) 4259, 4316
BfuAI ACCTGC 1 cut(s) 746
BfuI GTATCC 1 cut(s) 75
BglI GCCNNNNNGGC 2 cut(s) 1084, 1990
BglII AGATCT 1 cut(s) 4530
BlnI CCTAGG 1 cut(s) 433
BlpI GCTNAGC 1 cut(s) 2047
Bme1390I CCNGG 5 cut(s) 1946, 2315, 2636, 2669, 4349
Bme18I GGWCC 5 cut(s) 430, 736, 921, 2239, 3294
BmeRI GACNNNNNGTC 2 cut(s) 2404, 3005
BmeT110I CYCGRG 4 cut(s) 935, 1218, 3183, 4338
BmgBI CACGTC 2 cut(s) 3490, 4223
BmiI GGNNCC 9 cut(s) 85, 649, 1872, 2229, 2240, 2624, 3124, 3750, 3966
BmrFI CCNGG 5 cut(s) 1946, 2315, 2636, 2669, 4349
BmrI ACTGGG 1 cut(s) 1506
BmuI ACTGGG 1 cut(s) 1506
BpiI GAAGAC 4 cut(s) 153, 2058, 2672, 2679
BplI GAGNNNNNCTC 2 cut(s) 1348, 1380
BpmI CTGGAG 4 cut(s) 636, 2543, 2724, 4370
Bpu10I CCTNAGC 3 cut(s) 748, 4074, 4670
Bpu1102I GCTNAGC 1 cut(s) 2047
Bpu14I TTCGAA 1 cut(s) 3684
BpuEI CTTGAG 6 cut(s) 752, 1124, 2282, 2311, 4298, 4498
BpuMI CCSGG 2 cut(s) 2636, 2669
Bsa29I ATCGAT 1 cut(s) 4375
BsaAI YACGTR 2 cut(s) 2518, 4388
BsaBI GATNNNNATC 2 cut(s) 4381, 4535
BsaHI GRCGYC 2 cut(s) 309, 3123
BsaI GGTCTC 2 cut(s) 2811, 4265
BsaWI WCCGGW 3 cut(s) 707, 3291, 3994
BsaXI ACNNNNNCTCC 6 cut(s) 933, 963, 2158, 2188, 4490, 4520
Bsc4I CCNNNNNNNGG 9 cut(s) 54, 575, 1879, 3115, 3746, 3747, 3994, 4500, 4873
Bse118I RCCGGY 2 cut(s) 707, 966
Bse21I CCTNAGG 1 cut(s) 4683
Bse3DI GCAATG 2 cut(s) 148, 4397
Bse8I GATNNNNATC 2 cut(s) 4381, 4535
BseAI TCCGGA 1 cut(s) 3994
BseBI CCWGG 3 cut(s) 1946, 2315, 4349
BseCI ATCGAT 1 cut(s) 4375
BseJI GATNNNNATC 2 cut(s) 4381, 4535
BseLI CCNNNNNNNGG 9 cut(s) 54, 575, 1879, 3115, 3746, 3747, 3994, 4500, 4873
BseMI GCAATG 2 cut(s) 148, 4397
BseRI GAGGAG 2 cut(s) 1920, 3201
BseSI GKGCMC 6 cut(s) 651, 1874, 2719, 3204, 4228, 4759
BseYI CCCAGC 1 cut(s) 1277
BsgI GTGCAG 1 cut(s) 2073
Bsh1236I CGCG 3 cut(s) 36, 55, 4734
Bsh1285I CGRYCG 3 cut(s) 113, 983, 4326
BshFI GGCC 9 cut(s) 649, 1872, 2253, 2757, 3662, 3751, 4265, 4553, 4866
BshNI GGYRCC 1 cut(s) 3122
BshTI ACCGGT 1 cut(s) 707
BshVI ATCGAT 1 cut(s) 4375
BsiEI CGRYCG 3 cut(s) 113, 983, 4326
BsiHKAI GWGCWC 8 cut(s) 93, 936, 2048, 2268, 2719, 3204, 4228, 4759
BsiHKCI CYCGRG 4 cut(s) 935, 1218, 3183, 4338
BsiSI CCGG 6 cut(s) 708, 967, 2635, 2669, 3292, 3995
BslFI GGGAC 5 cut(s) 541, 1949, 2089, 2252, 3421
BslI CCNNNNNNNGG 9 cut(s) 54, 575, 1879, 3115, 3746, 3747, 3994, 4500, 4873
BsmAI GTCTC 9 cut(s) 402, 455, 684, 1020, 1241, 2636, 2811, 4032, 4265
BsmBI CGTCTC 2 cut(s) 1020, 1241
BsmFI GGGAC 5 cut(s) 541, 1949, 2089, 2252, 3421
BsmI GAATGC 6 cut(s) 331, 1034, 1092, 2010, 2562, 4674
BsnI GGCC 9 cut(s) 649, 1872, 2253, 2757, 3662, 3751, 4265, 4553, 4866
Bso31I GGTCTC 2 cut(s) 2811, 4265
BsoBI CYCGRG 4 cut(s) 935, 1218, 3183, 4338
Bsp119I TTCGAA 1 cut(s) 3684
Bsp120I GGGCCC 2 cut(s) 647, 1870
Bsp13I TCCGGA 1 cut(s) 3994
Bsp1407I TGTACA 1 cut(s) 3618
Bsp1720I GCTNAGC 1 cut(s) 2047
Bsp19I CCATGG 1 cut(s) 2800
BspANI GGCC 9 cut(s) 649, 1872, 2253, 2757, 3662, 3751, 4265, 4553, 4866
BspDI ATCGAT 1 cut(s) 4375
BspEI TCCGGA 1 cut(s) 3994
BspFNI CGCG 3 cut(s) 36, 55, 4734
BspHI TCATGA 2 cut(s) 238, 2941
BspLI GGNNCC 9 cut(s) 85, 649, 1872, 2229, 2240, 2624, 3124, 3750, 3966
BspMAI CTGCAG 6 cut(s) 502, 757, 769, 1963, 3423, 3973
BspMI ACCTGC 1 cut(s) 746
BspPI GGATC 7 cut(s) 36, 613, 769, 2325, 2357, 2830, 3928
BspT104I TTCGAA 1 cut(s) 3684
BspT107I GGYRCC 1 cut(s) 3122
BspTI CTTAAG 2 cut(s) 4259, 4316
BspTNI GGTCTC 2 cut(s) 2811, 4265
BsrDI GCAATG 2 cut(s) 148, 4397
BsrFI RCCGGY 2 cut(s) 707, 966
BsrGI TGTACA 1 cut(s) 3618
BssAI RCCGGY 2 cut(s) 707, 966
BssNI GRCGYC 2 cut(s) 309, 3123
BssSI CACGAG 1 cut(s) 1052
BssT1I CCWWGG 7 cut(s) 433, 651, 794, 2617, 2800, 3268, 3543
Bst2BI CACGAG 1 cut(s) 1052
Bst2UI CCWGG 3 cut(s) 1946, 2315, 4349
Bst6I CTCTTC 5 cut(s) 795, 1841, 3293, 3312, 4629
BstACI GRCGYC 2 cut(s) 309, 3123
BstAFI CTTAAG 2 cut(s) 4259, 4316
BstAPI GCANNNNNTGC 2 cut(s) 1274, 1337
BstAUI TGTACA 1 cut(s) 3618
BstBAI YACGTR 2 cut(s) 2518, 4388
BstBI TTCGAA 1 cut(s) 3684
BstC8I GCNNGC 7 cut(s) 93, 221, 516, 2268, 3283, 3354, 4428
BstDSI CCRYGG 3 cut(s) 2800, 3812, 4326
BstENI CCTNNNNNAGG 1 cut(s) 4871
BstFNI CGCG 3 cut(s) 36, 55, 4734
BstH2I RGCGCY 3 cut(s) 2054, 2306, 3126
BstHHI GCGC 6 cut(s) 38, 55, 991, 2053, 2305, 3125
BstMAI GTCTC 9 cut(s) 402, 455, 684, 1020, 1241, 2636, 2811, 4032, 4265
BstMCI CGRYCG 3 cut(s) 113, 983, 4326
BstNI CCWGG 3 cut(s) 1946, 2315, 4349
BstNSI RCATGY 8 cut(s) 782, 1644, 3079, 3208, 3625, 3704, 4395, 4458
BstSCI CCNGG 5 cut(s) 1944, 2313, 2634, 2667, 4347
BstSFI CTRYAG 9 cut(s) 498, 753, 765, 1659, 1959, 2548, 3419, 3969, 4413
BstSLI GKGCMC 6 cut(s) 651, 1874, 2719, 3204, 4228, 4759
BstUI CGCG 3 cut(s) 36, 55, 4734
BstV2I GAAGAC 4 cut(s) 153, 2058, 2672, 2679
BstX2I RGATCY 4 cut(s) 28, 761, 2362, 4530
BstXI CCANNNNNNTGG 2 cut(s) 2249, 4549
BstYI RGATCY 4 cut(s) 28, 761, 2362, 4530
Bsu15I ATCGAT 1 cut(s) 4375
Bsu36I CCTNAGG 1 cut(s) 4683
BsuI GTATCC 1 cut(s) 75
BsuRI GGCC 9 cut(s) 649, 1872, 2253, 2757, 3662, 3751, 4265, 4553, 4866
BsuTUI ATCGAT 1 cut(s) 4375
BtgI CCRYGG 3 cut(s) 2800, 3812, 4326
BtgZI GCGATG 4 cut(s) 897, 1073, 1671, 1828
BtrI CACGTC 2 cut(s) 3490, 4223
BtsI GCAGTG 3 cut(s) 2140, 3248, 3853
BtsIMutI CAGTG 6 cut(s) 133, 1342, 2140, 3248, 3439, 3853
BveI ACCTGC 1 cut(s) 746
Cac8I GCNNGC 7 cut(s) 93, 221, 516, 2268, 3283, 3354, 4428
CaiI CAGNNNCTG 2 cut(s) 614, 4012
CciI TCATGA 2 cut(s) 238, 2941
CfoI GCGC 6 cut(s) 38, 55, 991, 2053, 2305, 3125
Cfr10I RCCGGY 2 cut(s) 707, 966
ClaI ATCGAT 1 cut(s) 4375
CseI GACGC 6 cut(s) 317, 872, 1565, 1726, 2600, 4343
CspAI ACCGGT 1 cut(s) 707
CspCI CAANNNNNGTGG 4 cut(s) 1704, 1739, 2913, 2948
DinI GGCGCC 1 cut(s) 3124
DraIII CACNNNGTG 1 cut(s) 2224
DriI GACNNNNNGTC 2 cut(s) 2404, 3005
EaeI YGGCCR 2 cut(s) 2251, 4551
Eam1104I CTCTTC 5 cut(s) 795, 1841, 3293, 3312, 4629
Eam1105I GACNNNNNGTC 2 cut(s) 2404, 3005
EarI CTCTTC 5 cut(s) 795, 1841, 3293, 3312, 4629
EciI GGCGGA 3 cut(s) 34, 41, 73
Ecl136II GAGCTC 3 cut(s) 934, 2046, 2266
Eco130I CCWWGG 7 cut(s) 433, 651, 794, 2617, 2800, 3268, 3543
Eco147I AGGCCT 1 cut(s) 4866
Eco24I GRGCYC 5 cut(s) 651, 936, 1874, 2048, 2268
Eco31I GGTCTC 2 cut(s) 2811, 4265
Eco32I GATATC 3 cut(s) 1622, 2196, 3470
Eco47I GGWCC 5 cut(s) 430, 736, 921, 2239, 3294
Eco47III AGCGCT 1 cut(s) 2052
Eco53kI GAGCTC 3 cut(s) 934, 2046, 2266
Eco57I CTGAAG 4 cut(s) 493, 1788, 2112, 3944
Eco81I CCTNAGG 1 cut(s) 4683
Eco88I CYCGRG 4 cut(s) 935, 1218, 3183, 4338
EcoICRI GAGCTC 3 cut(s) 934, 2046, 2266
EcoNI CCTNNNNNAGG 1 cut(s) 4871
EcoO109I RGGNCCY 4 cut(s) 647, 648, 736, 3749
EcoRI GAATTC 2 cut(s) 3013, 4562
EcoRII CCWGG 3 cut(s) 1944, 2313, 4347
EcoRV GATATC 3 cut(s) 1622, 2196, 3470
EcoT14I CCWWGG 7 cut(s) 433, 651, 794, 2617, 2800, 3268, 3543
EcoT22I ATGCAT 1 cut(s) 1333
EcoT38I GRGCYC 5 cut(s) 651, 936, 1874, 2048, 2268
EgeI GGCGCC 1 cut(s) 3124
EheI GGCGCC 1 cut(s) 3124
ErhI CCWWGG 7 cut(s) 433, 651, 794, 2617, 2800, 3268, 3543
Esp3I CGTCTC 2 cut(s) 1020, 1241
FalI AAGNNNNNCTT 8 cut(s) 138, 170, 1380, 1412, 2664, 2696, 3440, 3472
FaqI GGGAC 5 cut(s) 541, 1949, 2089, 2252, 3421
FauI CCCGC 4 cut(s) 76, 1107, 3290, 4806
FauNDI CATATG 3 cut(s) 2097, 2327, 2886
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 2 cut(s) 2397, 4322
FriOI GRGCYC 5 cut(s) 651, 936, 1874, 2048, 2268
FspI TGCGCA 1 cut(s) 990
GlaI GCGC 6 cut(s) 37, 54, 990, 2052, 2304, 3124
GsaI CCCAGC 1 cut(s) 1281
GsuI CTGGAG 4 cut(s) 636, 2543, 2724, 4370
HaeII RGCGCY 3 cut(s) 2054, 2306, 3126
HaeIII GGCC 9 cut(s) 649, 1872, 2253, 2757, 3662, 3751, 4265, 4553, 4866
HapII CCGG 6 cut(s) 708, 967, 2635, 2669, 3292, 3995
HgaI GACGC 6 cut(s) 317, 872, 1565, 1726, 2600, 4343
HhaI GCGC 6 cut(s) 38, 55, 991, 2053, 2305, 3125
Hin1I GRCGYC 2 cut(s) 309, 3123
Hin6I GCGC 6 cut(s) 36, 53, 989, 2051, 2303, 3123
HinP1I GCGC 6 cut(s) 36, 53, 989, 2051, 2303, 3123
HincII GTYRAC 3 cut(s) 1545, 2409, 4323
HindII GTYRAC 3 cut(s) 1545, 2409, 4323
HindIII AAGCTT 5 cut(s) 104, 194, 227, 1970, 3316
HpaII CCGG 6 cut(s) 708, 967, 2635, 2669, 3292, 3995
HphI GGTGA 8 cut(s) 878, 2428, 3542, 3559, 3587, 4139, 4531, 4828
Hpy99I CGWCG 2 cut(s) 1742, 4592
Hsp92I GRCGYC 2 cut(s) 309, 3123
HspAI GCGC 6 cut(s) 36, 53, 989, 2051, 2303, 3123
KasI GGCGCC 1 cut(s) 3122
Kpn2I TCCGGA 1 cut(s) 3994
Ksp22I TGATCA 1 cut(s) 535
LmnI GCTCC 7 cut(s) 83, 1450, 2166, 2705, 2926, 3970, 4351
MaeIII GTNAC 8 cut(s) 610, 781, 884, 1581, 2698, 3521, 3530, 4745
MfeI CAATTG 2 cut(s) 1383, 3626
MflI RGATCY 4 cut(s) 28, 761, 2362, 4530
MlsI TGGCCA 2 cut(s) 2253, 4553
MluNI TGGCCA 2 cut(s) 2253, 4553
Mly113I GGCGCC 1 cut(s) 3123
MlyI GAGTC 8 cut(s) 189, 361, 563, 634, 2393, 2638, 2787, 3353
MmeI TCCRAC 2 cut(s) 3096, 3917
Mox20I TGGCCA 2 cut(s) 2253, 4553
Mph1103I ATGCAT 1 cut(s) 1333
MroI TCCGGA 1 cut(s) 3994
MroXI GAANNNNTTC 3 cut(s) 150, 354, 3888
MscI TGGCCA 2 cut(s) 2253, 4553
MslI CAYNNNNRTG 2 cut(s) 4390, 4547
Msp20I TGGCCA 2 cut(s) 2253, 4553
MspA1I CMGCKG 6 cut(s) 752, 1271, 2035, 3020, 3428, 4073
MspCI CTTAAG 2 cut(s) 4259, 4316
MspI CCGG 6 cut(s) 708, 967, 2635, 2669, 3292, 3995
MspR9I CCNGG 5 cut(s) 1946, 2315, 2636, 2669, 4349
MunI CAATTG 2 cut(s) 1383, 3626
Mva1269I GAATGC 6 cut(s) 331, 1034, 1092, 2010, 2562, 4674
MvaI CCWGG 3 cut(s) 1946, 2315, 4349
MvnI CGCG 3 cut(s) 36, 55, 4734
NarI GGCGCC 1 cut(s) 3123
NciI CCSGG 2 cut(s) 2636, 2669
NcoI CCATGG 1 cut(s) 2800
NdeI CATATG 3 cut(s) 2097, 2327, 2886
NlaIV GGNNCC 9 cut(s) 85, 649, 1872, 2229, 2240, 2624, 3124, 3750, 3966
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 4 cut(s) 884, 1581, 3530, 4745
NsbI TGCGCA 1 cut(s) 990
NsiI ATGCAT 1 cut(s) 1333
NspI RCATGY 8 cut(s) 782, 1644, 3079, 3208, 3625, 3704, 4395, 4458
NspV TTCGAA 1 cut(s) 3684
PaeR7I CTCGAG 3 cut(s) 935, 1218, 3183
PagI TCATGA 2 cut(s) 238, 2941
PaqCI CACCTGC 1 cut(s) 746
PceI AGGCCT 1 cut(s) 4866
PciI ACATGT 1 cut(s) 3700
PcsI WCGNNNNNNNCGW 2 cut(s) 4302, 4731
PctI GAATGC 6 cut(s) 331, 1034, 1092, 2010, 2562, 4674
PdmI GAANNNNTTC 3 cut(s) 150, 354, 3888
PfeI GAWTC 8 cut(s) 1147, 1315, 1457, 1532, 2829, 2843, 3559, 3821
PfoI TCCNGGA 2 cut(s) 2667, 4347
PinAI ACCGGT 1 cut(s) 707
PleI GAGTC 8 cut(s) 188, 361, 562, 633, 2393, 2637, 2787, 3352
PluTI GGCGCC 1 cut(s) 3126
PpsI GAGTC 8 cut(s) 188, 361, 562, 633, 2393, 2637, 2787, 3352
Ppu21I YACGTR 2 cut(s) 2518, 4388
PpuMI RGGWCCY 1 cut(s) 736
PscI ACATGT 1 cut(s) 3700
PsiI TTATAA 1 cut(s) 998
Psp124BI GAGCTC 3 cut(s) 936, 2048, 2268
Psp1406I AACGTT 3 cut(s) 1376, 1762, 4725
Psp5II RGGWCCY 1 cut(s) 736
Psp6I CCWGG 3 cut(s) 1944, 2313, 4347
PspFI CCCAGC 1 cut(s) 1277
PspGI CCWGG 3 cut(s) 1944, 2313, 4347
PspN4I GGNNCC 9 cut(s) 85, 649, 1872, 2229, 2240, 2624, 3124, 3750, 3966
PspOMI GGGCCC 2 cut(s) 647, 1870
PspPPI RGGWCCY 1 cut(s) 736
PspXI VCTCGAGB 3 cut(s) 935, 1218, 3183
PstI CTGCAG 6 cut(s) 502, 757, 769, 1963, 3423, 3973
PstNI CAGNNNCTG 2 cut(s) 614, 4012
PsuI RGATCY 4 cut(s) 28, 761, 2362, 4530
PvuII CAGCTG 6 cut(s) 752, 1271, 2035, 3020, 3428, 4073
RseI CAYNNNNRTG 2 cut(s) 4390, 4547
SacI GAGCTC 3 cut(s) 936, 2048, 2268
SalI GTCGAC 1 cut(s) 4321
SchI GAGTC 8 cut(s) 189, 361, 563, 634, 2393, 2638, 2787, 3353
ScrFI CCNGG 5 cut(s) 1946, 2315, 2636, 2669, 4349
SfcI CTRYAG 9 cut(s) 498, 753, 765, 1659, 1959, 2548, 3419, 3969, 4413
SfoI GGCGCC 1 cut(s) 3124
Sfr274I CTCGAG 3 cut(s) 935, 1218, 3183
SfuI TTCGAA 1 cut(s) 3684
SinI GGWCC 5 cut(s) 430, 736, 921, 2239, 3294
SlaI CTCGAG 3 cut(s) 935, 1218, 3183
SmiMI CAYNNNNRTG 2 cut(s) 4390, 4547
SseBI AGGCCT 1 cut(s) 4866
SspDI GGCGCC 1 cut(s) 3122
SspI AATATT 2 cut(s) 355, 390
SstI GAGCTC 3 cut(s) 936, 2048, 2268
StuI AGGCCT 1 cut(s) 4866
StyD4I CCNGG 5 cut(s) 1944, 2313, 2634, 2667, 4347
StyI CCWWGG 7 cut(s) 433, 651, 794, 2617, 2800, 3268, 3543
TaqII GACCGA 1 cut(s) 99
TatI WGTACW 5 cut(s) 1771, 2437, 3259, 3618, 3712
TauI GCSGC 2 cut(s) 17, 58
TfiI GAWTC 8 cut(s) 1147, 1315, 1457, 1532, 2829, 2843, 3559, 3821
TscAI CASTG 6 cut(s) 133, 1342, 2140, 3248, 3446, 3853
TseFI GTSAC 4 cut(s) 884, 1581, 3530, 4745
Tsp45I GTSAC 4 cut(s) 884, 1581, 3530, 4745
TspGWI ACGGA 4 cut(s) 96, 1159, 4692, 4871
TspRI CASTG 6 cut(s) 133, 1342, 2140, 3248, 3446, 3853
Vha464I CTTAAG 2 cut(s) 4259, 4316
VneI GTGCAC 4 cut(s) 2715, 3200, 4224, 4755
VpaK11BI GGWCC 5 cut(s) 430, 736, 921, 2239, 3294
XagI CCTNNNNNAGG 1 cut(s) 4871
XapI RAATTY 9 cut(s) 572, 1697, 2026, 2505, 3013, 3679, 4237, 4562, 4833
XbaI TCTAGA 1 cut(s) 4369
XceI RCATGY 8 cut(s) 782, 1644, 3079, 3208, 3625, 3704, 4395, 4458
XhoI CTCGAG 3 cut(s) 935, 1218, 3183
XmaJI CCTAGG 1 cut(s) 433
XmiI GTMKAC 2 cut(s) 2397, 4322
XmnI GAANNNNTTC 3 cut(s) 150, 354, 3888
Zsp2I ATGCAT 1 cut(s) 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.