MD04G1218000.v1.1

Protein MON2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007304
Physical Location & Seq
Reverse (-)
30043764 .. 30059656
15893 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1218000.v1.1.491

Sequence Viewer

Length: 4980 bp
ATGGCTTTCATGGCGGTTTTGGAGTCGGATCTTCGCGCGCTTTCCGCCGAAGCCCGCCGTCGCTACCCCGCCGTCAAGGACGGCGCTGAGCACGCAATTATCAAGCTTCGTAATTTGTCGAGTCCCAGTGAGATTGCGCAGAATGAGGACATATTGCGAATCTTTTTGATGGCTTGTGAGGTTAAAACTGTGAAGCTGAGTGTTATCGGACTTTCGTGCTTACAAAAGCTAATATCTCACGACGCTGTGGCTCCGTCAGCCCTGAATGAGATTCTTTCTACACTGAAGGATCATGCAGAAATGGCTGACGAAAGTGTTCAACTTAAGACCCTGCAGACTGTGTTAATCATATTGCAGTCACCTTTACATCCCGAGACTGAGGACAATATGGCTCAAGCTCTTGGTATCTGCCTTCGGCTGCTTGAAAACAACCGGTCCTCGGATAGCGTACGGAATACTGCTGCAGCTACTTTCAGGCAAGCCGTAGCACTGATTTTTGATCATGTGATTTGCGCTGAGACTCTTCCTGCTGGCAAGCTAAGTTCTGGAGGTTACATCTCTCGCACCAGTCTAGTTAGTGGCGATGTTAGTTCCAGTATCAATCTTTCAGAGTCATTGGATAAATCTCTTTCTGGACGGTCAGCATTGATGAGGGAGACCTTAACTAAAGCTGGAAAGCTTGGACTTCGTCTGCTTGAAGACCTGACAGCTCTTGCTGCAGGTGGATCTGCAATTTGGTTACGAGTGGGCTCTCTTCAGCGGTCCTTTGCACTTGATATACTGGAGTTTGTTTTGTCCAATTATGTGGCTGTTTTCAGAACCCTGCTTCCTTATGAGCAGGTTCTGCGCCACCAGATATGTTCACTTCTAATGACTTCACTTCGTACCAATGCTGAGATTGAAGGGGAAGCAGGAGAGCCTTCTTTTCGTCGTTTGGTCTTGCGATCAGTTGCCCATATTATCAGGCTATACAGTTCATCCCTTATCACAGAATGTGAGGTTTTCCTTAGTATGTTGGTGAAGGCCACATTTCTTGATCTGCCATTGTGGCATCGTATTCTTGTTCTTGAGATCCTGAGGGGCTTTTGTGTGGACGCAAGGACATTGCGGATTCTTTTCCGAAATTTTGATATGCATCCGAAGAACACCAATGTTGTGGAGGGCATGGTCAAAGCTCTTGCTAGAGTTGTTTCAAGTGTACAGTTTCAAGAGACTAGTGATGAGAGCCTGGCAGCTGTTGCAGGGATGTTTAACAGCAAAGCTAAAGGAATTGAGTGGAGCCTTGATAACGATGCATCCAATGCTGCGGTCTTGGTTGCCAGTGAAGCTCATTCGATAACATTGGCAGTTGAAGGCCTTCTGGGTATTGTCTTTACTGTGGCTACACTGACGGATGAAGCAGTAGATTCTGGGGAGATTGAATCCCCCAGACATGATTATGACCCACCTGCAAAGTGTTCTGGAAATACTTCTATTCTCTGCCTCTCAATGGTTGATTCATTATGGTTGACTATACTAGATGCATTGTCCTTTATTTTGTCAAGGTCACAAGGAGAGGCTATTGTGTTGGAAATACTGAAGGGATATCAAGCCTTCACCCAGGCATGCGGGGTTCTTCGTGCCGTAGAACCTTTGAATTCTTTTCTTGCATCCCTTTGCAAATTTACGATCAATTTTCCCATTGAATCTGAAAGAAGGAGTGCTGTACAATCTCCTGGGTCAAAACGCTTTGAACTATTAGTTGATCAAAGGGAAAGCGTTGTTCTTACTCCCAAGAATGTCCAGGCTTTAAGAACTCTCTTCAACATTGCTCATCGACTACATAATGTATTGGGTCCATCCTGGGTCTTGGTATTAGAAACACTAGCAGCTCTGGACCGAGCAATCCATTCACCACATGCCACCACGCAGGAGGTGTCCACGGGTGTGCCAAAGCTTACAAGGGAATCATCTGGTCAATCCAGCGACTTAAACATTCTTTCTTCTTTGAATTCTCAGCTGTTCGAGAGCTCAGCACTAATGCACATATCTGCAGTAAAGTCCCTTCTTTCTGCCCTATGCCAACTGTCACAGCAATGCATGGCTGGGATTACAAGTGGTACCGTACCCACATCAAGTCAAAAAGTTGACAACATAAACTTTTCAGTGGAGAGAATAATATCCATCCTTGTCAATAATCTACACAGAGTGGAGCCATTGTGGGATCAAGTCGTTAGCCACTTTCTTGAGCTTGCAGATAAGTCTAACCAGCATTTACGTAATATGGCCCTTGATGCATTAGATGAATCGATATGTGCTGTTTTAGGTTCAGACCAGTTCCAGGACAGCACAACAACCAGATCTAGAGCATCTCAGAATATGGAAACTGGACTTACACACTTGGGATCACTTGAATGTGCTGTCATATCGCCACTAAGGGTTCTTTATTTATCTACTCAAAGTGTTGAATTACGTGCTGGATCTTTGAAAATTCTTCTGCATGTTTTAGAGAGGCACGGGGAGAAATTGCACTACAGTTGGCCGGATATTCTTGAACTGTTGAGGTCTGTAGCGGATTCTTCAGAGAAGGAACTTGTTACTCTGGCCTTTCAGAGCCTACGTGTGATTATGAATGATGGACTTTCAGCTATACCTGCCGACTGCCTTCCTGTATGTGTAGATGTGACTGGAGCATACAGTGCTCAAAAGACAGAACTGAATATAAGCTTGACAGCGATAGGCCTTTTATGGACAACAACCGACTTTATAGCCAAAGGTCTGACGCATGGGATTGGAGAGGAAAAGGAAACAGGGATTTCTGATGTGCAATTGGATAGTGAAAAGCCAAAAGAACAAGCATCCGATGTATCAGACAATGTAAATGACCAAGCTCCCTTGTTAAATGTAGTTGATCGTGACAGGTTGCTTTTCTCTGCTTTCTCGTTACTTCAGAAGCTTGGAGCTGATGAAAGGCCAGAGGTTAGAAATTCAGCGATCAGGACACTTTTTCAGACACTTGGAAGTCATGGGCAAAAACTTTCGAAAAGCATGTGGGAGGATTGCCTTTGGAATTACATTTTTCCTACACTAGATCGTGCTTCTCACATGGCTGAGACTTCATCAAAAGATGAATGGCACGGGAAAGAACTCGGAACTCGGGGAGGAAAGGCCGTTCATATGCTCATACATCATAGTCGCAACACAGCTCAGAAACAGTGGGATGAAACACTTGTACTAGTGCTTGGAGGCATAGCACGTATATTACGTTCATTCTTTCCTTTTCTTAGAAGCTTAAACAACTTTTGGTCAGGATGGGAGTCTTTGCTTTTATTTGTCAAGAACAGCATTTTAAATGGTAGCAAAGAGGTTGCTATTGCTGCAATAAATTGTTTACTGACACCCGTTCTTTCTCATTCTTCTAAGGGGAACCTGCCAAGGCCTTACCTTGAATCAGTTCTTGATGCCTACGAGGTTGTTCTCCAAAAGTCAACAAACCTGAGTGGCAATGCAGCTATCAAGGTGAAGCAGGAGATTTTGCACAGTCTCGGGGAACTTTATGTGCAAGCCCAGAGGATGTTTGACGATAATTTGTATCAGCAGTTGCTTGCAGTCATAGATTCAGCTGTTAAACAAGCCATAATTGGCAACGATAATTGTGACACTGAGTTTGGACATGTTCCACTTGTATTACGTTCTGTGCTGGAGATTCTTCCGATGTTACGTCCAACAGAGCTCATTTCTTCCATGTGGCTTATTCTTCTTCGGGATTTTTTGCAATATCTGCCAAGATTACATTCTTCTGTACAAAAGGAGGAAGATGGCGCAGAGGAAGCCAGCACTAGTGACCAAGTTCCAGACGATCACGTGAGAATAAAACGTGAAATTCCTAATGGGACATCTTCCATATCTTCAAGCAGAGTAGAACGTTCTCCAAGTTCTGGATTAAAGACATCTGTAATAGCAGGCATCCCCAATTATATGTTTGCAGAAAAGCTTGTTCCCCTGCTGGTCGATCTTTTTCTACAGGCCCCAGCAGTTCAAAAGTATATCTTGTATTCTGAAATTATTCAAAGTCTTGGGAGGTGTATGACTACAAGAAGAGACAATCCAGATGGTGCTGTTTGGAGATTAGCTGTTGAAGGCTTCAACCGTATTCTGGTTGATGATGCCAGCAAATCGGCTATAAATTCTGGACTAGATTCAGGTGCTAGTAAGCCTGCAAGAACACGTATATGGAAGGAAGTTGCCGATGTTTATGAAGTATTCCTTGTGGGTTACTGTGGACGAGCTCTTCCCTCTGATGATTCTTTCTCAACTGTGGATGTAAAAGCTGATGAGTCCCTTGAGATGATCGTCTTAGACGTACTCGGAGACAGGATTCTAAAGTCACCTATCGATGCACCTTCCGATATTTTGCATCGTCTAGTTTCCACCTTGGACCGATGCGCATCACGTACTTGTTCCTTACCTGTCGATTTTGTGGAACTTATGCCTTCCCACTGCAGCAGATTTTCCTTGAAATGTTTACAGAAGCTATTCTCCTTGAGCAGTTACGACAACAAATCCCATGATTGGAATTCAGCGAGATCCGAAGTGAGCAAAATTGCAGTCATGGTACTGATAACCAGATGCGAATACATCTTGAGCAGATTTCTAATTGACGAGAACGACTTAGGTGGACGCCCATTGCCGGCTGCAAGACTTGAAGAAATTATTTACGTTCTTGAAGAGTTGGCGCGGCTGATAATTCATTCAGATACTGCATCCGTTCTTCCCTTGAAGGCACATTTGAAAAGTGCCCTAGAAAACGAAAAGAATCACGACATACGCCCGCATCTGCTTGTGCTATTTCCCTCATTTTCCGAGCTCGTAGTATCAAGAGAAGCAAGAATAAGAGGGTCAGTGCAAGTCTTATTCAGACTGATCTCCAAAGAATTGGGTCTTGACCGAGCTTCCATAAACGGGCAAAATCCGGAAGAATTTGTTCCAAGGACACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7_N

Protein Analysis

1660

Amino Acids

182.98

Weight (kDa)

5.76

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 3 - 170 1.7e-38 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 230 - 385 2.7e-20 Mon2/Sec7/BIG1-like, HUS domain
Sec7-like_HDS PF09324 800 - 866 1.8e-06 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 872 - 1133 8.2e-48 C-terminal region of Mon2 protein
Mon2_C PF16206 1318 - 1576 2.9e-14 C-terminal region of Mon2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 710, 1456
Acc16I TGCGCA 2 cut(s) 138, 4426
Acc36I ACCTGC 5 cut(s) 710, 829, 1456, 2641, 3417
Acc65I GGTACC 1 cut(s) 2099
AccB1I GGYRCC 1 cut(s) 2099
AccB7I CCANNNNNTGG 1 cut(s) 3917
AccII CGCG 3 cut(s) 36, 38, 4717
AccIII TCCGGA 1 cut(s) 4951
AclI AACGTT 1 cut(s) 3904
AclWI GGATC 8 cut(s) 36, 297, 733, 1066, 2211, 2392, 2467, 4560
AcoI YGGCCR 1 cut(s) 2519
AcuI CTGAAG 5 cut(s) 305, 740, 1598, 2544, 2912
AcvI CACGTG 1 cut(s) 3844
AcyI GRCGYC 1 cut(s) 4660
AdeI CACNNNGTG 1 cut(s) 2188
AfiI CCNNNNNNNGG 8 cut(s) 439, 1489, 2320, 3917, 4135, 4551, 4669, 4941
AflII CTTAAG 1 cut(s) 323
AflIII ACRYGT 3 cut(s) 2599, 3652, 4205
AgeI ACCGGT 1 cut(s) 432
AhlI ACTAGT 3 cut(s) 1214, 3214, 3818
AjnI CCWGG 6 cut(s) 1227, 1599, 1714, 1782, 1841, 2319
AjuI GAANNNNNNNTTGG 2 cut(s) 3757, 3789
AleI CACNNNNGTG 1 cut(s) 1925
AloI GAACNNNNNNTCC 4 cut(s) 811, 843, 3135, 3167
Alw21I GWGCWC 6 cut(s) 93, 2012, 2683, 3714, 4270, 4848
Alw26I GTCTC 8 cut(s) 368, 512, 650, 1205, 3086, 3527, 4074, 4344
AlwI GGATC 8 cut(s) 36, 297, 733, 1066, 2211, 2392, 2467, 4560
AlwNI CAGNNNCTG 2 cut(s) 844, 4739
Ama87I CYCGRG 3 cut(s) 371, 3135, 3524
Aor13HI TCCGGA 1 cut(s) 4951
ArsI GACNNNNNNTTYG 4 cut(s) 3629, 3661, 4572, 4604
AsiGI ACCGGT 1 cut(s) 432
Asp700I GAANNNNTTC 7 cut(s) 315, 1356, 1468, 3432, 4044, 4514, 4962
Asp718I GGTACC 1 cut(s) 2099
AspLEI GCGC 9 cut(s) 38, 40, 86, 139, 515, 849, 3803, 4427, 4717
AspS9I GGNCC 7 cut(s) 435, 762, 1835, 1876, 2266, 4006, 4417
AsuHPI GGTGA 6 cut(s) 351, 1030, 1588, 1884, 3511, 4359
AsuII TTCGAA 1 cut(s) 3020
AvaI CYCGRG 3 cut(s) 371, 3135, 3524
AvaII GGWCC 5 cut(s) 435, 762, 1835, 1876, 4417
AxyI CCTNAGG 1 cut(s) 1076
BaeGI GKGCMC 1 cut(s) 4780
BanI GGYRCC 1 cut(s) 2099
BanII GRGCYC 5 cut(s) 752, 2012, 3714, 4270, 4848
BarI GAAGNNNNNNTAC 2 cut(s) 2028, 2060
BbrPI CACGTG 1 cut(s) 3844
BbsI GAAGAC 1 cut(s) 705
Bbv12I GWGCWC 6 cut(s) 93, 2012, 2683, 3714, 4270, 4848
BccI CCATC 7 cut(s) 163, 1846, 2171, 2609, 3285, 3791, 4085
BceAI ACGGC 6 cut(s) 42, 56, 97, 467, 1607, 3134
BciT130I CCWGG 6 cut(s) 1229, 1601, 1716, 1784, 1843, 2321
BclI TGATCA 2 cut(s) 499, 1744
BcoDI GTCTC 8 cut(s) 368, 512, 650, 1205, 3086, 3527, 4074, 4344
BcuI ACTAGT 3 cut(s) 1214, 3214, 3818
BfmI CTRYAG 8 cut(s) 332, 462, 717, 2031, 2512, 2547, 4001, 4480
BfoI RGCGCY 1 cut(s) 87
BfrI CTTAAG 1 cut(s) 323
BfuAI ACCTGC 5 cut(s) 710, 829, 1456, 2641, 3417
BglI GCCNNNNNGGC 1 cut(s) 1048
BglII AGATCT 1 cut(s) 2339
BlpI GCTNAGC 2 cut(s) 87, 2011
Bme1390I CCNGG 6 cut(s) 1229, 1601, 1716, 1784, 1843, 2321
Bme18I GGWCC 5 cut(s) 435, 762, 1835, 1876, 4417
BmeT110I CYCGRG 3 cut(s) 371, 3135, 3524
BmgT120I GGNCC 7 cut(s) 435, 762, 1835, 1876, 2266, 4006, 4417
BmiI GGNNCC 7 cut(s) 252, 1280, 1836, 2101, 2193, 3407, 4008
BmrFI CCNGG 6 cut(s) 1229, 1601, 1716, 1784, 1843, 2321
BmrI ACTGGG 1 cut(s) 120
BmuI ACTGGG 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 705
BpmI CTGGAG 4 cut(s) 567, 803, 2688, 3701
Bpu1102I GCTNAGC 2 cut(s) 87, 2011
Bpu14I TTCGAA 1 cut(s) 3020
BpuEI CTTGAG 6 cut(s) 378, 1088, 2246, 4343, 4543, 4642
Bsa29I ATCGAT 2 cut(s) 2288, 4374
BsaAI YACGTR 7 cut(s) 2258, 2453, 2600, 3236, 3844, 4208, 4433
BsaBI GATNNNNATC 2 cut(s) 1134, 4426
BsaHI GRCGYC 1 cut(s) 4660
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 8 cut(s) 438, 1599, 1715, 1842, 1920, 3413, 4413, 4967
BsaWI WCCGGW 2 cut(s) 432, 4951
BsaXI ACNNNNNCTCC 2 cut(s) 2183, 2213
Bsc4I CCNNNNNNNGG 8 cut(s) 439, 1489, 2320, 3917, 4135, 4551, 4669, 4941
Bse118I RCCGGY 2 cut(s) 432, 4669
Bse1I ACTGG 8 cut(s) 126, 567, 594, 786, 1320, 2314, 2371, 2671
Bse21I CCTNAGG 1 cut(s) 1076
Bse3DI GCAATG 5 cut(s) 1103, 1806, 2081, 3490, 4664
Bse8I GATNNNNATC 2 cut(s) 1134, 4426
BseAI TCCGGA 1 cut(s) 4951
BseBI CCWGG 6 cut(s) 1229, 1601, 1716, 1784, 1843, 2321
BseCI ATCGAT 2 cut(s) 2288, 4374
BseDI CCNNGG 8 cut(s) 438, 1599, 1715, 1842, 1920, 3413, 4413, 4967
BseJI GATNNNNATC 2 cut(s) 1134, 4426
BseLI CCNNNNNNNGG 8 cut(s) 439, 1489, 2320, 3917, 4135, 4551, 4669, 4941
BseMI GCAATG 5 cut(s) 1103, 1806, 2081, 3490, 4664
BseNI ACTGG 8 cut(s) 126, 567, 594, 786, 1320, 2314, 2371, 2671
BsePI GCGCGC 1 cut(s) 36
BseSI GKGCMC 1 cut(s) 4780
BseYI CCCAGC 2 cut(s) 2084, 4009
Bsh1236I CGCG 3 cut(s) 36, 38, 4717
BshNI GGYRCC 1 cut(s) 2099
BshTI ACCGGT 1 cut(s) 432
BshVI ATCGAT 2 cut(s) 2288, 4374
BsiHKAI GWGCWC 6 cut(s) 93, 2012, 2683, 3714, 4270, 4848
BsiHKCI CYCGRG 3 cut(s) 371, 3135, 3524
BsiSI CCGG 4 cut(s) 433, 2522, 4670, 4952
BsiWI CGTACG 1 cut(s) 448
BslFI GGGAC 4 cut(s) 108, 2026, 3886, 4303
BslI CCNNNNNNNGG 8 cut(s) 439, 1489, 2320, 3917, 4135, 4551, 4669, 4941
BsmAI GTCTC 8 cut(s) 368, 512, 650, 1205, 3086, 3527, 4074, 4344
BsmFI GGGAC 4 cut(s) 108, 2026, 3886, 4303
Bso31I GGTCTC 1 cut(s) 650
BsoBI CYCGRG 3 cut(s) 371, 3135, 3524
Bsp119I TTCGAA 1 cut(s) 3020
Bsp1286I GDGCHC 8 cut(s) 93, 752, 2012, 2683, 3714, 4270, 4780, 4848
Bsp13I TCCGGA 1 cut(s) 4951
Bsp1407I TGTACA 3 cut(s) 1198, 1705, 3781
Bsp1720I GCTNAGC 2 cut(s) 87, 2011
BspDI ATCGAT 2 cut(s) 2288, 4374
BspEI TCCGGA 1 cut(s) 4951
BspFNI CGCG 3 cut(s) 36, 38, 4717
BspLI GGNNCC 7 cut(s) 252, 1280, 1836, 2101, 2193, 3407, 4008
BspMAI CTGCAG 5 cut(s) 336, 466, 721, 2035, 4484
BspMI ACCTGC 5 cut(s) 710, 829, 1456, 2641, 3417
BspPI GGATC 8 cut(s) 36, 297, 733, 1066, 2211, 2392, 2467, 4560
BspQI GCTCTTC 1 cut(s) 4275
BspT104I TTCGAA 1 cut(s) 3020
BspT107I GGYRCC 1 cut(s) 2099
BspTI CTTAAG 1 cut(s) 323
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 5 cut(s) 1103, 1806, 2081, 3490, 4664
BsrFI RCCGGY 2 cut(s) 432, 4669
BsrGI TGTACA 3 cut(s) 1198, 1705, 3781
BsrI ACTGG 8 cut(s) 126, 567, 594, 786, 1320, 2314, 2371, 2671
BssAI RCCGGY 2 cut(s) 432, 4669
BssECI CCNNGG 8 cut(s) 438, 1599, 1715, 1842, 1920, 3413, 4413, 4967
BssHII GCGCGC 1 cut(s) 36
BssNI GRCGYC 1 cut(s) 4660
BssT1I CCWWGG 3 cut(s) 3413, 4413, 4967
Bst2UI CCWGG 6 cut(s) 1229, 1601, 1716, 1784, 1843, 2321
Bst6I CTCTTC 6 cut(s) 528, 759, 1805, 4072, 4275, 4701
BstACI GRCGYC 1 cut(s) 4660
BstAFI CTTAAG 1 cut(s) 323
BstAPI GCANNNNNTGC 5 cut(s) 844, 1238, 1301, 2678, 3760
BstAUI TGTACA 3 cut(s) 1198, 1705, 3781
BstBAI YACGTR 7 cut(s) 2258, 2453, 2600, 3236, 3844, 4208, 4433
BstBI TTCGAA 1 cut(s) 3020
BstDSI CCRYGG 1 cut(s) 1920
BstFNI CGCG 3 cut(s) 36, 38, 4717
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 9 cut(s) 38, 40, 86, 139, 515, 849, 3803, 4427, 4717
BstMAI GTCTC 8 cut(s) 368, 512, 650, 1205, 3086, 3527, 4074, 4344
BstNI CCWGG 6 cut(s) 1229, 1601, 1716, 1784, 1843, 2321
BstNSI RCATGY 5 cut(s) 1608, 1901, 2483, 3031, 3656
BstSCI CCNGG 6 cut(s) 1227, 1599, 1714, 1782, 1841, 2319
BstSFI CTRYAG 8 cut(s) 332, 462, 717, 2031, 2512, 2547, 4001, 4480
BstSLI GKGCMC 1 cut(s) 4780
BstSNI TACGTA 1 cut(s) 2258
BstUI CGCG 3 cut(s) 36, 38, 4717
BstV2I GAAGAC 1 cut(s) 705
BstX2I RGATCY 6 cut(s) 28, 725, 1071, 2339, 2459, 4565
BstXI CCANNNNNNTGG 3 cut(s) 805, 1156, 4915
BstYI RGATCY 6 cut(s) 28, 725, 1071, 2339, 2459, 4565
Bsu15I ATCGAT 2 cut(s) 2288, 4374
Bsu36I CCTNAGG 1 cut(s) 1076
BsuTUI ATCGAT 2 cut(s) 2288, 4374
BtgI CCRYGG 1 cut(s) 1920
BtgZI GCGATG 1 cut(s) 597
BtsI GCAGTG 1 cut(s) 4477
BveI ACCTGC 5 cut(s) 710, 829, 1456, 2641, 3417
CaiI CAGNNNCTG 2 cut(s) 844, 4739
CfoI GCGC 9 cut(s) 38, 40, 86, 139, 515, 849, 3803, 4427, 4717
Cfr10I RCCGGY 2 cut(s) 432, 4669
Cfr13I GGNCC 7 cut(s) 435, 762, 1835, 1876, 2266, 4006, 4417
ClaI ATCGAT 2 cut(s) 2288, 4374
CseI GACGC 4 cut(s) 251, 1103, 2770, 4668
CspAI ACCGGT 1 cut(s) 432
CspCI CAANNNNNGTGG 2 cut(s) 1015, 1050
DraI TTTAAA 1 cut(s) 3330
DraIII CACNNNGTG 1 cut(s) 2188
EaeI YGGCCR 1 cut(s) 2519
Eam1104I CTCTTC 6 cut(s) 528, 759, 1805, 4072, 4275, 4701
EarI CTCTTC 6 cut(s) 528, 759, 1805, 4072, 4275, 4701
EciI GGCGGA 1 cut(s) 34
Ecl136II GAGCTC 4 cut(s) 2010, 3712, 4268, 4846
Eco105I TACGTA 1 cut(s) 2258
Eco130I CCWWGG 3 cut(s) 3413, 4413, 4967
Eco147I AGGCCT 3 cut(s) 1356, 2721, 3418
Eco24I GRGCYC 5 cut(s) 752, 2012, 3714, 4270, 4848
Eco31I GGTCTC 1 cut(s) 650
Eco32I GATATC 1 cut(s) 1586
Eco47I GGWCC 5 cut(s) 435, 762, 1835, 1876, 4417
Eco53kI GAGCTC 4 cut(s) 2010, 3712, 4268, 4846
Eco57I CTGAAG 5 cut(s) 305, 740, 1598, 2544, 2912
Eco72I CACGTG 1 cut(s) 3844
Eco81I CCTNAGG 1 cut(s) 1076
Eco88I CYCGRG 3 cut(s) 371, 3135, 3524
EcoICRI GAGCTC 4 cut(s) 2010, 3712, 4268, 4846
EcoO109I RGGNCCY 1 cut(s) 4006
EcoRI GAATTC 3 cut(s) 1636, 1990, 4555
EcoRII CCWGG 6 cut(s) 1227, 1599, 1714, 1782, 1841, 2319
EcoRV GATATC 1 cut(s) 1586
EcoT14I CCWWGG 3 cut(s) 3413, 4413, 4967
EcoT22I ATGCAT 5 cut(s) 1137, 1297, 1525, 2081, 2278
EcoT38I GRGCYC 5 cut(s) 752, 2012, 3714, 4270, 4848
ErhI CCWWGG 3 cut(s) 3413, 4413, 4967
FalI AAGNNNNNCTT 4 cut(s) 4013, 4045, 4230, 4262
FaqI GGGAC 4 cut(s) 108, 2026, 3886, 4303
FauI CCCGC 4 cut(s) 62, 76, 1601, 4818
FauNDI CATATG 1 cut(s) 3156
FbaI TGATCA 2 cut(s) 499, 1744
FriOI GRGCYC 5 cut(s) 752, 2012, 3714, 4270, 4848
FspAI RTGCGCAY 1 cut(s) 4426
FspI TGCGCA 2 cut(s) 138, 4426
GlaI GCGC 9 cut(s) 37, 39, 85, 138, 514, 848, 3802, 4426, 4716
GsaI CCCAGC 2 cut(s) 2088, 4013
GsuI CTGGAG 4 cut(s) 567, 803, 2688, 3701
HaeII RGCGCY 1 cut(s) 87
HapII CCGG 4 cut(s) 433, 2522, 4670, 4952
HgaI GACGC 4 cut(s) 251, 1103, 2770, 4668
HhaI GCGC 9 cut(s) 38, 40, 86, 139, 515, 849, 3803, 4427, 4717
Hin1I GRCGYC 1 cut(s) 4660
Hin6I GCGC 9 cut(s) 36, 38, 84, 137, 513, 847, 3801, 4425, 4715
HinP1I GCGC 9 cut(s) 36, 38, 84, 137, 513, 847, 3801, 4425, 4715
HincII GTYRAC 3 cut(s) 1509, 2128, 3468
HindII GTYRAC 3 cut(s) 1509, 2128, 3468
HindIII AAGCTT 7 cut(s) 104, 677, 1934, 2704, 2933, 3268, 3971
HpaII CCGG 4 cut(s) 433, 2522, 4670, 4952
HphI GGTGA 6 cut(s) 351, 1030, 1588, 1884, 3511, 4359
Hpy99I CGWCG 3 cut(s) 63, 245, 933
Hsp92I GRCGYC 1 cut(s) 4660
HspAI GCGC 9 cut(s) 36, 38, 84, 137, 513, 847, 3801, 4425, 4715
Kpn2I TCCGGA 1 cut(s) 4951
KpnI GGTACC 1 cut(s) 2103
KroI GCCGGC 1 cut(s) 4669
KroNI GCCGGC 1 cut(s) 4671
Ksp22I TGATCA 2 cut(s) 499, 1744
LguI GCTCTTC 1 cut(s) 4275
LmnI GCTCC 6 cut(s) 256, 1278, 2191, 2669, 2875, 2939
MfeI CAATTG 1 cut(s) 2807
MflI RGATCY 6 cut(s) 28, 725, 1071, 2339, 2459, 4565
MhlI GDGCHC 8 cut(s) 93, 752, 2012, 2683, 3714, 4270, 4780, 4848
MlyI GAGTC 6 cut(s) 32, 130, 514, 620, 3305, 4325
MmeI TCCRAC 3 cut(s) 6, 1548, 3728
Mph1103I ATGCAT 5 cut(s) 1137, 1297, 1525, 2081, 2278
MroI TCCGGA 1 cut(s) 4951
MroNI GCCGGC 1 cut(s) 4669
MroXI GAANNNNTTC 7 cut(s) 315, 1356, 1468, 3432, 4044, 4514, 4962
MslI CAYNNNNRTG 5 cut(s) 1437, 1925, 2074, 2392, 4210
MspA1I CMGCKG 4 cut(s) 760, 1235, 1999, 3602
MspCI CTTAAG 1 cut(s) 323
MspI CCGG 4 cut(s) 433, 2522, 4670, 4952
MspR9I CCNGG 6 cut(s) 1229, 1601, 1716, 1784, 1843, 2321
MunI CAATTG 1 cut(s) 2807
MvaI CCWGG 6 cut(s) 1229, 1601, 1716, 1784, 1843, 2321
MvnI CGCG 3 cut(s) 36, 38, 4717
NaeI GCCGGC 1 cut(s) 4671
NdeI CATATG 1 cut(s) 3156
NgoMIV GCCGGC 1 cut(s) 4669
NlaIV GGNNCC 7 cut(s) 252, 1280, 1836, 2101, 2193, 3407, 4008
NmuCI GTSAC 8 cut(s) 357, 1545, 2067, 2662, 2894, 3635, 3821, 4365
NsbI TGCGCA 2 cut(s) 138, 4426
NsiI ATGCAT 5 cut(s) 1137, 1297, 1525, 2081, 2278
NspI RCATGY 5 cut(s) 1608, 1901, 2483, 3031, 3656
NspV TTCGAA 1 cut(s) 3020
OliI CACNNNNGTG 1 cut(s) 1925
PaeI GCATGC 1 cut(s) 1608
PaqCI CACCTGC 2 cut(s) 710, 1456
PauI GCGCGC 1 cut(s) 36
PceI AGGCCT 3 cut(s) 1356, 2721, 3418
PciI ACATGT 1 cut(s) 3652
PciSI GCTCTTC 1 cut(s) 4275
PcsI WCGNNNNNNNCGW 2 cut(s) 3241, 4338
PdiI GCCGGC 1 cut(s) 4671
PdmI GAANNNNTTC 7 cut(s) 315, 1356, 1468, 3432, 4044, 4514, 4962
Pfl23II CGTACG 1 cut(s) 448
PflFI GACNNNGTC 1 cut(s) 687
PflMI CCANNNNNTGG 1 cut(s) 3917
PfoI TCCNGGA 1 cut(s) 2319
PinAI ACCGGT 1 cut(s) 432
PleI GAGTC 6 cut(s) 31, 129, 514, 619, 3304, 4324
PmaCI CACGTG 1 cut(s) 3844
PmlI CACGTG 1 cut(s) 3844
PpsI GAGTC 6 cut(s) 31, 129, 514, 619, 3304, 4324
Ppu21I YACGTR 7 cut(s) 2258, 2453, 2600, 3236, 3844, 4208, 4433
PscI ACATGT 1 cut(s) 3652
Psp124BI GAGCTC 4 cut(s) 2012, 3714, 4270, 4848
Psp1406I AACGTT 1 cut(s) 3904
Psp6I CCWGG 6 cut(s) 1227, 1599, 1714, 1782, 1841, 2319
PspCI CACGTG 1 cut(s) 3844
PspFI CCCAGC 2 cut(s) 2084, 4009
PspGI CCWGG 6 cut(s) 1227, 1599, 1714, 1782, 1841, 2319
PspLI CGTACG 1 cut(s) 448
PspN4I GGNNCC 7 cut(s) 252, 1280, 1836, 2101, 2193, 3407, 4008
PspPI GGNCC 7 cut(s) 435, 762, 1835, 1876, 2266, 4006, 4417
PsrI GAACNNNNNNTAC 2 cut(s) 3229, 3261
PstI CTGCAG 5 cut(s) 336, 466, 721, 2035, 4484
PstNI CAGNNNCTG 2 cut(s) 844, 4739
PsuI RGATCY 6 cut(s) 28, 725, 1071, 2339, 2459, 4565
PsyI GACNNNGTC 1 cut(s) 687
PteI GCGCGC 1 cut(s) 36
PvuII CAGCTG 3 cut(s) 1235, 1999, 3602
RseI CAYNNNNRTG 5 cut(s) 1437, 1925, 2074, 2392, 4210
SacI GAGCTC 4 cut(s) 2012, 3714, 4270, 4848
SapI GCTCTTC 1 cut(s) 4275
Sau96I GGNCC 7 cut(s) 435, 762, 1835, 1876, 2266, 4006, 4417
SchI GAGTC 6 cut(s) 32, 130, 514, 620, 3305, 4325
ScrFI CCNGG 6 cut(s) 1229, 1601, 1716, 1784, 1843, 2321
SduI GDGCHC 8 cut(s) 93, 752, 2012, 2683, 3714, 4270, 4780, 4848
SfcI CTRYAG 8 cut(s) 332, 462, 717, 2031, 2512, 2547, 4001, 4480
SfuI TTCGAA 1 cut(s) 3020
SinI GGWCC 5 cut(s) 435, 762, 1835, 1876, 4417
SmiMI CAYNNNNRTG 5 cut(s) 1437, 1925, 2074, 2392, 4210
SmlI CTYRAG 7 cut(s) 323, 393, 1067, 2225, 4322, 4522, 4621
SmoI CTYRAG 7 cut(s) 323, 393, 1067, 2225, 4322, 4522, 4621
SnaBI TACGTA 1 cut(s) 2258
SpeI ACTAGT 3 cut(s) 1214, 3214, 3818
SphI GCATGC 1 cut(s) 1608
SseBI AGGCCT 3 cut(s) 1356, 2721, 3418
SstI GAGCTC 4 cut(s) 2012, 3714, 4270, 4848
StuI AGGCCT 3 cut(s) 1356, 2721, 3418
StyD4I CCNGG 6 cut(s) 1227, 1599, 1714, 1782, 1841, 2319
StyI CCWWGG 3 cut(s) 3413, 4413, 4967
TaqI TCGA 9 cut(s) 119, 1334, 1816, 2004, 2288, 3020, 3990, 4374, 4452
TaqII GACCGA 3 cut(s) 1893, 4434, 4941
TatI WGTACW 4 cut(s) 1198, 1705, 3211, 3781
TauI GCSGC 1 cut(s) 4720
TseFI GTSAC 8 cut(s) 357, 1545, 2067, 2662, 2894, 3635, 3821, 4365
Tsp45I GTSAC 8 cut(s) 357, 1545, 2067, 2662, 2894, 3635, 3821, 4365
TspGWI ACGGA 4 cut(s) 243, 466, 1406, 4735
Tth111I GACNNNGTC 1 cut(s) 687
Van91I CCANNNNNTGG 1 cut(s) 3917
Vha464I CTTAAG 1 cut(s) 323
VpaK11BI GGWCC 5 cut(s) 435, 762, 1835, 1876, 4417
XbaI TCTAGA 1 cut(s) 2342
XceI RCATGY 5 cut(s) 1608, 1901, 2483, 3031, 3656
XmnI GAANNNNTTC 7 cut(s) 315, 1356, 1468, 3432, 4044, 4514, 4962
Zsp2I ATGCAT 5 cut(s) 1137, 1297, 1525, 2081, 2278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.