Rw3G009160

Protein MON2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0007304
Physical Location & Seq
Forward (+)
8720568 .. 8741467
20900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G009160.1

Sequence Viewer

Length: 4968 bp
ATGGCTTTCATGGCGGTTTTGGAGTCAGATCTTCGCGCTCTCTCCGCCGAGGCTCGCCGTCGCTACCCTGCTGTCAAAGACGGCGCTGAGCACGCAATTATAAAGCTCCGTTCTTTGTCAAGCCCTGGTGAGATTGCGCAGAATGAGGACATATTGAGAATCTTCCTGATGGCTTGTGAGGTTAAAACTGTGAAGTTGAGTGTTATTGGACTCTCGTGTCTACAGAAGCTTATATCTCATGATGCTGTTGCTTCATCAGCTCTGCACGAGATACTTTGTACATTGAAGGATCATGCTGAAATGACCGATGAGAGTGTTCAACTTAAGACCCTGCAGACTGTATTGATAATATTGCAGTCACCTTTACACCCTCAGAATGAGGACGATATGGCTCAAGCTCTTGGTATATGCCTTCGGCTTCTCGAAAATAGGTCCCCTGACAGTGTGCGCAATACTGCTGCAGCTACTTTCAGGCAAGCTGTAGCACTGATTTTTGATCATGTGGTTTGCGCTGAGACTCTTCCTGCCGGCAAGCTTAGTTCTGGAGGTTACATCTCTCGGACCAGTCCAGTTAGTGGTGATGTTAGTTGCAGCATAAATCTTTCAGAGTCTTTGGATGGATCTGTTTCTGGACAGTCATCATTGATGAGGGAGACACTAACTAAAGCTGGAAAGCTTGGGCTTCGTCTGCTTGAAGACCTGACAGCTCTTGCTGCAGGTGGATCTGCAATCTGGTTACGTGTCAGTTCTCTACAGCGGTCCTTTGCACTTGACATACTGGAGTTTGTTCTGTCCAACTATGTGGCTGTTTTCCGAACCTTGCTTCCTTATGAGCAGGTCCTGCGTTACCAGATATGTTCAATTCTAATGACCTCACTTCGTACAAATGCGGAGCTTGAAGGGGAAGCAGGAGAGCCGTCATTTCGACGCTTGGTCTTGCGGTCAGTTGCCCATATCATCCGGCTTTACAGTTCATCCCTTATCACTGAATGTGAGGTCTTCCTTAGTATGTTGGTGAAGGTCACATTTCTTGATTTGCCATTATGGCATCGTATTCTTGTTCTTGAGATCCTGAGGGGCTTTTGTGTGGAGGCAAGGACATTGCGGATTCTTTTCCGAAACTTTGATATGAATCCCAAGAACACCAACGTTGTGGAGGGCATGGTGAAAGCTCTTGCTAGAGTAGTTTCAAGTGTACAGGTTCAAGAGACTAGTGAAGAGAGCCTCGCAGCTGTTGCAGGAATGTTTAACAGCAAAGCTAAAGGAGTTGAGTGGAGCCTTGATAATGATGCATCCAATGCTGCAGTATTGGTTGCTAGTGAAGCTCATTGTATAACCTTGGCAGTCGAAGGTCTGCTCGGTGTAGTCTTCACAGTAGCTACATTGACGGATGAAGCAGTAGATTCCGGAGAGATTGAATCCCCCAGATGTGACTATGATCCACCTGCAAAGAAGACTGGAAATGCTGCTCTTCTCTGTCTCTCAATGGTTGATTCATTATGGTTGACGATACTAGATGCATTCTCCTTTATTTTGTCAAGGTCACAAGGTGAGGCTATTGTGTTGGAAATACTGAAGGGATATCAGGCCTTCACCCAGGCATGCGGAGTTCTTGGTGCTGTAGAACCTTTGAACTCCTTTTTAGCATCACTTTGCAAATTTACAATCATTTTCCCCGTTGAAGCAGAAAAAAGGAGCATTACTTTACAATCTCCTGGGTCCAAACGGTCTGAACAAGTAGTTGATCAAAGGGAAAGTGTTGTTCTCACCCCTAAGAATGTCCAGGCCTTAAGAACTCTCTTCAACATTGCTCATAGATTACACAATGTGTTGGGCCCATCCTGGGTTTTGGTATTAGAAACCCTAGCAGCCCTAGACCGCGCAATCCATTCTCCGCATGCTACCACACAGGAGGTGTCCACGGCTGTGCCAAAACTTACAAGAGAATCATCTGGTCAATCCAGTGACTTAAATATTCTTTCATCCTTGAATTCTCAGCTGTTTGAGAGCTCAGCACTGATGCACATATCTGCAGTAAAATCCCTTCTTTGTGCACTGGGCCAACTGTCACAGCAATGCATGGCTGGGATTTCAAATGGATCTGTACCTGCTTCAAGTCAAAAAGTTGGAAACATAAACTTTTCAGTGGAGAGAATGATATCCATCCTAGTCAATAATCTACACAGAGTGGAGCCATTGTGGGATCAAGTTGTTGGCCACTTTCTTGAGCTTGCAGAAAATTCTAACCAGCATTTACGTAACATGGCCCTTGATGCATTAGATGAATCAATATGTGCTGTTTTAGGTTCAGACAAGTTCCCAGACAACACAACAAGCAGATTCAATGGCTCATCTCAGAGTATGGATACTGGGGTTACAGATTTGGGATCACTTGAGTGTGCTGTCATATCTCCACTAAGGGTTCTTTATCTTTCTACTCAAAGTGTTGACTCACGTACTGGATCTTTGAAAATTCTTCTGCATGTTTTAGAGAGGCATGGGGAGAAATTACACTACAGTTGGCCAAATATTCTTGAAATGTTGAGGTCTGTAGCGGACTCTTCAGATAAGGAACTCATTACTCTGGGCTTTCAGTGCCTGCGGGTGATTATGAATGACGGACTTTCTACTATACCTGCAGACTGCCTTCAAGTATGTGTAGATGTGACTGGAGCATACAGTGCTCAAAAGACAGAGCTGAATATAAGTCTGACTGCAATAGGCCTCTTATGGACAACAACTGACTTTATTGTGAAAGCTCTAATCCATGGACCTGGGGCGGAGAAGAAAACAGGGATTTCTGATGTGCATCCTATTCTAAAGCCATTGGATGGCGACATACCAAAAGAACAAACAATCAATGGCTCAGATAATGCCAATGAGCAAGTTCCCTCATTAACTATAGTTGACAGTGACAGACTGCTTTTCTCTGTTTTCTCATTACTTCAAAAGCTTGGAGCCGATGAGAGGCCAGAGGTTAGAAATTCAGCTGTCAGGACATTGTTTCAGACACTTGGAAGTCACGGGCAAAAACTTTCGAAAAGCATGTGGGAAGATTGCCTTTGGAATTATGTGTTTCCTACACTCGATCGTGCTTCTCACATGGCTGCAACTTCATCGAAAGATGAATGGCATGGGAAAGAACTTGGAACTCGTGGAGGAAAAGCAGTTCATATGCTCATACATCACAGTCGCAACACAGCTCAGAAACAGTGGGATGAGACGTTAGTACTTGTCCTTGGTGGCGTAGCCCGTATTCTACGCTCATTCTTTCCTTTTCTTAGAAGTTTAAGCAATTTTTGGTCAGGATGGGAGTCTTTGCTTTTATTTGTGAAGAATAGTGTATTAAATGGAAGCAAAGAGGTTGCGCTTGCTGCAATAAATTGCTTACAGACACCTATTCTTTCTCATTCTTCCAAGGGGAACTTGCCGATGCCTTATCTTGAATCAGTACTTGATGTTTATGAGCTTGTTCTTCAAAATTCAACAAACCTGAGTGGAAATGCAGCCAGCAAGGTGAAGCAGGAGATTTTGCAGAGTCTTGGGGAACTATATGTGCAAGCACAAAGGATGTTTGATGATCGGTTGTATACACAGTTGCTTGCAGTCATACGCTTGGCTATGAAACATGCCATAGTTGCCAACGATAACTGTGAAACTGAATATGGAAATGTTCCACCTGTACTACGTACTGTGCTCGAGATCATTCCAATGTTATGTCCAACAGAGCACATTTCTTCAATGTGGCTTATTCTTCTTCGGGATTTTTCTCAATTTCTGCCAAGATTGAATTCTACCGTACAAATTGAGGAAGATGAAGCAGAGGAAGCTAGCACTAGTGACCGAGTTCCAGATACTCACTTGAAAATAAAACATGAAAGACCTAATGGGACGGCTTCGATGACTCTAGGAGTAGGAGATTCTCCTAGTTCTAGATTAAAGACATCCGTAACAGCCAGCATTCCTAATTATATGTTTGCGGAAAAGCTTGTTCCCTTGTTGGTAGATCTTTTCCTACGGGCTCCAGCAGTTGAAAAGTATATCTTGTATCCTGAAATTATTCAAAGTCTTGGGAGGTGTATGACTACAAGAAGAGACAATCCAGATGGTTCTCTTTGGAGATTAGCTGTCGAAGGCTTCAATCGCATTCTTGTTGATGATGCCAGCAACTCCACTGTAAATGCTGGATCAGATTCAGGTGCTAGTAAGCCTGCAAGAACACGTATATGGAAGGAAGTTGCCGATGTTTATGAAGTATTCCTTGTGGGTTATTGTGGCCGAGCTCTTCCTTCTGATTCCTTCTCAACAGTGGATGTAAAAGCTGACGAGTCCCTTGAGATGACTATCTTGCACATTCTTGGCGACAAGATTTTGAAGTCACCAATTGATGCACCGACCGATATTTTGCAGCGTCTCGTTTCCACTTTGGACCGTTGTGCTTCACGTACTGGTTCTTTACCTGTTGATTTTGTGGAACGTATGCCTTCCCACTGCAGCAGGTTTTCCTTGATTTGCTTACAGAAGTTGTTTTCCTTGAGCAGTTATGACACCAAAGACCATGATTGGAATTCAGCGAGAGCTGAGGTGAGTAAAATTGCAATCATGATACTGATGATGAGATGTGAATACATCTTAAGCAGATTTCTGAATGACGAAAATGATTTAGGTGGACGCCCATTGCCAGCAGCAAGACTTGAAGAAATCATATATGTTCTTGAAGAGTTGGGCCGACTAGTAATTCATTCAGATACTGCATCCATTCTTCCCTTGCAACCATATTTGAAAGGTGCTCTATCAAGAGACAAGAATCATGATGACCGTTCGCACCTTCTTGTTCTATTTCCATCCTTTTCTGAGCTCATAGTGTCAAGAGAAGCAAAAGTAAGAGCATTGGTTCAAGTCTTGTTTAGACTCATCAGCAAAGAATTGTCACTTGAAAGATTATATAGTCTGTGTACATTATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7_N

Protein Analysis

1655

Amino Acids

182.16

Weight (kDa)

5.75

Isoelectric Point (pI)

46.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 3 - 169 1.2e-38 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 229 - 384 1.2e-19 Mon2/Sec7/BIG1-like, HUS domain
Sec7-like_HDS PF09324 802 - 868 8.4e-07 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 873 - 1158 6.8e-49 C-terminal region of Mon2 protein
Mon2_C PF16206 1323 - 1580 1.3e-14 C-terminal region of Mon2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 101
AarI CACCTGC 2 cut(s) 707, 1453
AasI GACNNNNNNGTC 1 cut(s) 216
Acc16I TGCGCA 2 cut(s) 138, 449
Acc36I ACCTGC 6 cut(s) 707, 826, 1453, 2116, 2644, 4488
AccB7I CCANNNNNTGG 2 cut(s) 575, 2831
AccI GTMKAC 2 cut(s) 220, 3590
AccII CGCG 2 cut(s) 36, 1881
AccIII TCCGGA 1 cut(s) 1406
AclI AACGTT 1 cut(s) 1149
AcoI YGGCCR 3 cut(s) 2215, 2522, 4276
AcsI RAATTY 8 cut(s) 1658, 1990, 2239, 2472, 2983, 3481, 3790, 4567
AcuI CTGAAG 2 cut(s) 1595, 2547
AcyI GRCGYC 1 cut(s) 4672
AdeI CACNNNGTG 3 cut(s) 1550, 1828, 2188
AfiI CCNNNNNNNGG 4 cut(s) 575, 1843, 2831, 2967
AflII CTTAAG 3 cut(s) 323, 1789, 4633
AflIII ACRYGT 2 cut(s) 739, 4220
AhdI GACNNNNNGTC 1 cut(s) 932
AhlI ACTAGT 3 cut(s) 1211, 3836, 4732
AjnI CCWGG 6 cut(s) 124, 1596, 1714, 1782, 1841, 2773
AjuI GAANNNNNNNTTGG 2 cut(s) 3775, 3807
AleI CACNNNNGTG 2 cut(s) 1925, 2395
AloI GAACNNNNNNTCC 4 cut(s) 808, 840, 3153, 3185
Alw21I GWGCWC 9 cut(s) 93, 2012, 2056, 2686, 3699, 3732, 4285, 4792, 4860
Alw26I GTCTC 8 cut(s) 509, 647, 1202, 1484, 3215, 4089, 4418, 4794
Alw44I GTGCAC 1 cut(s) 2052
AlwNI CAGNNNCTG 3 cut(s) 841, 2599, 4751
Ama87I CYCGRG 1 cut(s) 3698
Aor13HI TCCGGA 1 cut(s) 1406
ApaI GGGCCC 1 cut(s) 1838
ApaLI GTGCAC 1 cut(s) 2052
ApoI RAATTY 8 cut(s) 1658, 1990, 2239, 2472, 2983, 3481, 3790, 4567
ArsI GACNNNNNNTTYG 2 cut(s) 4387, 4419
Asp700I GAANNNNTTC 1 cut(s) 4059
AspLEI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1883, 3370
AsuII TTCGAA 1 cut(s) 3038
AsuNHI GCTAGC 1 cut(s) 3830
AvaI CYCGRG 1 cut(s) 3698
AvaII GGWCC 7 cut(s) 432, 561, 759, 838, 1719, 2771, 4429
AxyI CCTNAGG 1 cut(s) 1073
BaeGI GKGCMC 2 cut(s) 1838, 2056
BaeI ACNNNNGTAYC 2 cut(s) 2358, 2391
BalI TGGCCA 2 cut(s) 2217, 2524
BanII GRGCYC 5 cut(s) 1838, 2012, 4024, 4285, 4860
BarI GAAGNNNNNNTAC 2 cut(s) 2028, 2060
BauI CACGAG 3 cut(s) 214, 266, 3153
BbsI GAAGAC 4 cut(s) 702, 991, 1360, 1460
Bbv12I GWGCWC 9 cut(s) 93, 2012, 2056, 2686, 3699, 3732, 4285, 4792, 4860
BbvCI CCTCAGC 1 cut(s) 4581
BccI CCATC 8 cut(s) 163, 611, 1846, 2171, 2825, 3303, 4100, 4852
BceAI ACGGC 5 cut(s) 42, 97, 901, 1938, 3909
BcgI CGANNNNNNTGC 2 cut(s) 3099, 3133
BciT130I CCWGG 6 cut(s) 126, 1598, 1716, 1784, 1843, 2775
BciVI GTATCC 2 cut(s) 2359, 4059
BclI TGATCA 2 cut(s) 496, 1744
BcoDI GTCTC 8 cut(s) 509, 647, 1202, 1484, 3215, 4089, 4418, 4794
BcuI ACTAGT 3 cut(s) 1211, 3836, 4732
BfoI RGCGCY 1 cut(s) 87
BfrI CTTAAG 3 cut(s) 323, 1789, 4633
BfuAI ACCTGC 6 cut(s) 707, 826, 1453, 2116, 2644, 4488
BfuI GTATCC 2 cut(s) 2359, 4059
BglI GCCNNNNNGGC 1 cut(s) 1045
BglII AGATCT 2 cut(s) 28, 4006
BlpI GCTNAGC 2 cut(s) 87, 2011
BmcAI AGTACT 2 cut(s) 3231, 3453
Bme1390I CCNGG 6 cut(s) 126, 1598, 1716, 1784, 1843, 2775
Bme18I GGWCC 7 cut(s) 432, 561, 759, 838, 1719, 2771, 4429
BmeRI GACNNNNNGTC 1 cut(s) 932
BmeT110I CYCGRG 1 cut(s) 3698
BmiI GGNNCC 7 cut(s) 434, 1277, 1720, 1836, 2193, 2959, 4023
BmrFI CCNGG 6 cut(s) 126, 1598, 1716, 1784, 1843, 2775
BmrI ACTGGG 2 cut(s) 2066, 2379
BmtI GCTAGC 1 cut(s) 3834
BmuI ACTGGG 2 cut(s) 2066, 2379
BpiI GAAGAC 4 cut(s) 702, 991, 1360, 1460
BpmI CTGGAG 4 cut(s) 564, 800, 2691, 4008
Bpu10I CCTNAGC 1 cut(s) 4581
Bpu1102I GCTNAGC 2 cut(s) 87, 2011
Bpu14I TTCGAA 1 cut(s) 3038
BpuEI CTTGAG 6 cut(s) 378, 1085, 2246, 2414, 4355, 4555
BsaAI YACGTR 6 cut(s) 740, 2258, 2456, 3689, 4223, 4445
BsaBI GATNNNNATC 4 cut(s) 1131, 2162, 2808, 4343
BsaHI GRCGYC 1 cut(s) 4672
BsaWI WCCGGW 1 cut(s) 1406
BsaXI ACNNNNNCTCC 2 cut(s) 2183, 2213
Bsc4I CCNNNNNNNGG 4 cut(s) 575, 1843, 2831, 2967
Bse118I RCCGGY 1 cut(s) 527
Bse21I CCTNAGG 1 cut(s) 1073
Bse3DI GCAATG 4 cut(s) 1100, 1806, 2081, 4676
Bse8I GATNNNNATC 4 cut(s) 1131, 2162, 2808, 4343
BseAI TCCGGA 1 cut(s) 1406
BseBI CCWGG 6 cut(s) 126, 1598, 1716, 1784, 1843, 2775
BseJI GATNNNNATC 4 cut(s) 1131, 2162, 2808, 4343
BseLI CCNNNNNNNGG 4 cut(s) 575, 1843, 2831, 2967
BseMI GCAATG 4 cut(s) 1100, 1806, 2081, 4676
BseSI GKGCMC 2 cut(s) 1838, 2056
BseYI CCCAGC 1 cut(s) 2084
BsgI GTGCAG 1 cut(s) 248
Bsh1236I CGCG 2 cut(s) 36, 1881
Bsh1285I CGRYCG 2 cut(s) 3091, 4398
BsiEI CGRYCG 2 cut(s) 3091, 4398
BsiHKAI GWGCWC 9 cut(s) 93, 2012, 2056, 2686, 3699, 3732, 4285, 4792, 4860
BsiHKCI CYCGRG 1 cut(s) 3698
BsiSI CCGG 3 cut(s) 528, 961, 1407
BslFI GGGAC 3 cut(s) 418, 3904, 4315
BslI CCNNNNNNNGG 4 cut(s) 575, 1843, 2831, 2967
BsmAI GTCTC 8 cut(s) 509, 647, 1202, 1484, 3215, 4089, 4418, 4794
BsmBI CGTCTC 2 cut(s) 3215, 4418
BsmFI GGGAC 3 cut(s) 418, 3904, 4315
BsmI GAATGC 3 cut(s) 1520, 3960, 4146
BsoBI CYCGRG 1 cut(s) 3698
Bsp119I TTCGAA 1 cut(s) 3038
Bsp120I GGGCCC 1 cut(s) 1834
Bsp13I TCCGGA 1 cut(s) 1406
Bsp1407I TGTACA 3 cut(s) 278, 1195, 4955
Bsp1720I GCTNAGC 2 cut(s) 87, 2011
Bsp19I CCATGG 1 cut(s) 2767
BspEI TCCGGA 1 cut(s) 1406
BspFNI CGCG 2 cut(s) 36, 1881
BspHI TCATGA 3 cut(s) 238, 4602, 4810
BspLI GGNNCC 7 cut(s) 434, 1277, 1720, 1836, 2193, 2959, 4023
BspMAI CTGCAG 7 cut(s) 336, 463, 718, 1306, 2035, 2641, 4496
BspMI ACCTGC 6 cut(s) 707, 826, 1453, 2116, 2644, 4488
BspOI GCTAGC 1 cut(s) 3834
BspQI GCTCTTC 2 cut(s) 1476, 4290
BspT104I TTCGAA 1 cut(s) 3038
BspTI CTTAAG 3 cut(s) 323, 1789, 4633
BsrDI GCAATG 4 cut(s) 1100, 1806, 2081, 4676
BsrFI RCCGGY 1 cut(s) 527
BsrGI TGTACA 3 cut(s) 278, 1195, 4955
BssAI RCCGGY 1 cut(s) 527
BssNAI GTATAC 1 cut(s) 3591
BssNI GRCGYC 1 cut(s) 4672
BssSI CACGAG 3 cut(s) 214, 266, 3153
BssT1I CCWWGG 4 cut(s) 1338, 2767, 3238, 3417
Bst1107I GTATAC 1 cut(s) 3591
Bst2BI CACGAG 3 cut(s) 214, 266, 3153
Bst2UI CCWGG 6 cut(s) 126, 1598, 1716, 1784, 1843, 2775
Bst6I CTCTTC 8 cut(s) 525, 1212, 1476, 1805, 2566, 4087, 4290, 4713
BstACI GRCGYC 1 cut(s) 4672
BstAFI CTTAAG 3 cut(s) 323, 1789, 4633
BstAPI GCANNNNNTGC 4 cut(s) 841, 1235, 1298, 2681
BstAUI TGTACA 3 cut(s) 278, 1195, 4955
BstBAI YACGTR 6 cut(s) 740, 2258, 2456, 3689, 4223, 4445
BstBI TTCGAA 1 cut(s) 3038
BstDSI CCRYGG 2 cut(s) 1920, 2767
BstFNI CGCG 2 cut(s) 36, 1881
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1883, 3370
BstMAI GTCTC 8 cut(s) 509, 647, 1202, 1484, 3215, 4089, 4418, 4794
BstMCI CGRYCG 2 cut(s) 3091, 4398
BstNI CCWGG 6 cut(s) 126, 1598, 1716, 1784, 1843, 2775
BstNSI RCATGY 5 cut(s) 1605, 1901, 2486, 3049, 3632
BstSCI CCNGG 6 cut(s) 124, 1596, 1714, 1782, 1841, 2773
BstSLI GKGCMC 2 cut(s) 1838, 2056
BstSNI TACGTA 2 cut(s) 2258, 3689
BstUI CGCG 2 cut(s) 36, 1881
BstV2I GAAGAC 4 cut(s) 702, 991, 1360, 1460
BstX2I RGATCY 7 cut(s) 28, 620, 722, 1068, 2099, 2462, 4006
BstXI CCANNNNNNTGG 3 cut(s) 802, 1153, 2774
BstYI RGATCY 7 cut(s) 28, 620, 722, 1068, 2099, 2462, 4006
BstZ17I GTATAC 1 cut(s) 3591
Bsu36I CCTNAGG 1 cut(s) 1073
BsuI GTATCC 2 cut(s) 2359, 4059
BtgI CCRYGG 2 cut(s) 1920, 2767
BtsI GCAGTG 1 cut(s) 4489
BveI ACCTGC 6 cut(s) 707, 826, 1453, 2116, 2644, 4488
CaiI CAGNNNCTG 3 cut(s) 841, 2599, 4751
CciI TCATGA 3 cut(s) 238, 4602, 4810
CfoI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1883, 3370
Cfr10I RCCGGY 1 cut(s) 527
CseI GACGC 3 cut(s) 936, 4400, 4680
DraIII CACNNNGTG 3 cut(s) 1550, 1828, 2188
DrdI GACNNNNNNGTC 1 cut(s) 216
DriI GACNNNNNGTC 1 cut(s) 932
DseDI GACNNNNNNGTC 1 cut(s) 216
EaeI YGGCCR 3 cut(s) 2215, 2522, 4276
Eam1104I CTCTTC 8 cut(s) 525, 1212, 1476, 1805, 2566, 4087, 4290, 4713
Eam1105I GACNNNNNGTC 1 cut(s) 932
EarI CTCTTC 8 cut(s) 525, 1212, 1476, 1805, 2566, 4087, 4290, 4713
EciI GGCGGA 2 cut(s) 34, 2795
Ecl136II GAGCTC 3 cut(s) 2010, 4283, 4858
Eco105I TACGTA 2 cut(s) 2258, 3689
Eco130I CCWWGG 4 cut(s) 1338, 2767, 3238, 3417
Eco147I AGGCCT 3 cut(s) 1589, 1787, 2724
Eco24I GRGCYC 5 cut(s) 1838, 2012, 4024, 4285, 4860
Eco32I GATATC 2 cut(s) 1583, 2160
Eco47I GGWCC 7 cut(s) 432, 561, 759, 838, 1719, 2771, 4429
Eco53kI GAGCTC 3 cut(s) 2010, 4283, 4858
Eco57I CTGAAG 2 cut(s) 1595, 2547
Eco81I CCTNAGG 1 cut(s) 1073
Eco88I CYCGRG 1 cut(s) 3698
EcoICRI GAGCTC 3 cut(s) 2010, 4283, 4858
EcoO109I RGGNCCY 2 cut(s) 432, 838
EcoRI GAATTC 3 cut(s) 1990, 3790, 4567
EcoRII CCWGG 6 cut(s) 124, 1596, 1714, 1782, 1841, 2773
EcoRV GATATC 2 cut(s) 1583, 2160
EcoT14I CCWWGG 4 cut(s) 1338, 2767, 3238, 3417
EcoT22I ATGCAT 4 cut(s) 1294, 1522, 2081, 2278
EcoT38I GRGCYC 5 cut(s) 1838, 2012, 4024, 4285, 4860
ErhI CCWWGG 4 cut(s) 1338, 2767, 3238, 3417
Esp3I CGTCTC 2 cut(s) 3215, 4418
FalI AAGNNNNNCTT 8 cut(s) 3045, 3077, 3410, 3442, 4028, 4060, 4245, 4277
FaqI GGGAC 3 cut(s) 418, 3904, 4315
FauI CCCGC 1 cut(s) 2595
FauNDI CATATG 1 cut(s) 3174
FbaI TGATCA 2 cut(s) 496, 1744
FblI GTMKAC 2 cut(s) 220, 3590
FriOI GRGCYC 5 cut(s) 1838, 2012, 4024, 4285, 4860
FspI TGCGCA 2 cut(s) 138, 449
GlaI GCGC 7 cut(s) 37, 85, 138, 449, 511, 1882, 3369
GsaI CCCAGC 1 cut(s) 2088
GsuI CTGGAG 4 cut(s) 564, 800, 2691, 4008
HaeII RGCGCY 1 cut(s) 87
HapII CCGG 3 cut(s) 528, 961, 1407
HgaI GACGC 3 cut(s) 936, 4400, 4680
HhaI GCGC 7 cut(s) 38, 86, 139, 450, 512, 1883, 3370
Hin1I GRCGYC 1 cut(s) 4672
Hin6I GCGC 7 cut(s) 36, 84, 137, 448, 510, 1881, 3368
HinP1I GCGC 7 cut(s) 36, 84, 137, 448, 510, 1881, 3368
HincII GTYRAC 3 cut(s) 1506, 2449, 2908
HindII GTYRAC 3 cut(s) 1506, 2449, 2908
HindIII AAGCTT 5 cut(s) 227, 533, 674, 2951, 3986
HpaII CCGG 3 cut(s) 528, 961, 1407
Hpy99I CGWCG 2 cut(s) 63, 930
HpyCH4IV ACGT 9 cut(s) 739, 1149, 2257, 2455, 3224, 3688, 4222, 4444, 4477
HpySE526I ACGT 9 cut(s) 739, 1149, 2257, 2455, 3224, 3688, 4222, 4444, 4477
Hsp92I GRCGYC 1 cut(s) 4672
HspAI GCGC 7 cut(s) 36, 84, 137, 448, 510, 1881, 3368
Kpn2I TCCGGA 1 cut(s) 1406
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
Ksp22I TGATCA 2 cut(s) 496, 1744
LguI GCTCTTC 2 cut(s) 1476, 4290
LmnI GCTCC 8 cut(s) 111, 892, 1275, 1695, 2191, 2672, 2957, 4027
MaeII ACGT 9 cut(s) 739, 1149, 2257, 2455, 3224, 3688, 4222, 4444, 4477
MfeI CAATTG 1 cut(s) 4383
MflI RGATCY 7 cut(s) 28, 620, 722, 1068, 2099, 2462, 4006
MlsI TGGCCA 2 cut(s) 2217, 2524
MluNI TGGCCA 2 cut(s) 2217, 2524
MmeI TCCRAC 4 cut(s) 819, 1545, 2107, 3746
Mox20I TGGCCA 2 cut(s) 2217, 2524
Mph1103I ATGCAT 4 cut(s) 1294, 1522, 2081, 2278
MroI TCCGGA 1 cut(s) 1406
MroNI GCCGGC 1 cut(s) 527
MroXI GAANNNNTTC 1 cut(s) 4059
MscI TGGCCA 2 cut(s) 2217, 2524
MslI CAYNNNNRTG 5 cut(s) 1925, 2074, 2395, 3710, 4225
Msp20I TGGCCA 2 cut(s) 2217, 2524
MspA1I CMGCKG 4 cut(s) 757, 1232, 1999, 2990
MspCI CTTAAG 3 cut(s) 323, 1789, 4633
MspI CCGG 3 cut(s) 528, 961, 1407
MspR9I CCNGG 6 cut(s) 126, 1598, 1716, 1784, 1843, 2775
MunI CAATTG 1 cut(s) 4383
Mva1269I GAATGC 3 cut(s) 1520, 3960, 4146
MvaI CCWGG 6 cut(s) 126, 1598, 1716, 1784, 1843, 2775
MvnI CGCG 2 cut(s) 36, 1881
NaeI GCCGGC 1 cut(s) 529
NcoI CCATGG 1 cut(s) 2767
NdeI CATATG 1 cut(s) 3174
NgoMIV GCCGGC 1 cut(s) 527
NheI GCTAGC 1 cut(s) 3830
NlaIV GGNNCC 7 cut(s) 434, 1277, 1720, 1836, 2193, 2959, 4023
NmeAIII GCCGAG 2 cut(s) 73, 4304
NsbI TGCGCA 2 cut(s) 138, 449
NsiI ATGCAT 4 cut(s) 1294, 1522, 2081, 2278
NspI RCATGY 5 cut(s) 1605, 1901, 2486, 3049, 3632
NspV TTCGAA 1 cut(s) 3038
OliI CACNNNNGTG 2 cut(s) 1925, 2395
PaeI GCATGC 2 cut(s) 1605, 1901
PaeR7I CTCGAG 1 cut(s) 3698
PagI TCATGA 3 cut(s) 238, 4602, 4810
PaqCI CACCTGC 2 cut(s) 707, 1453
PceI AGGCCT 3 cut(s) 1589, 1787, 2724
PciSI GCTCTTC 2 cut(s) 1476, 4290
PctI GAATGC 3 cut(s) 1520, 3960, 4146
PdiI GCCGGC 1 cut(s) 529
PdmI GAANNNNTTC 1 cut(s) 4059
PflMI CCANNNNNTGG 2 cut(s) 575, 2831
Ple19I CGATCG 1 cut(s) 3091
Ppu21I YACGTR 6 cut(s) 740, 2258, 2456, 3689, 4223, 4445
PpuMI RGGWCCY 2 cut(s) 432, 838
PsiI TTATAA 1 cut(s) 101
Psp124BI GAGCTC 3 cut(s) 2012, 4285, 4860
Psp1406I AACGTT 1 cut(s) 1149
Psp5II RGGWCCY 2 cut(s) 432, 838
Psp6I CCWGG 6 cut(s) 124, 1596, 1714, 1782, 1841, 2773
PspFI CCCAGC 1 cut(s) 2084
PspGI CCWGG 6 cut(s) 124, 1596, 1714, 1782, 1841, 2773
PspN4I GGNNCC 7 cut(s) 434, 1277, 1720, 1836, 2193, 2959, 4023
PspOMI GGGCCC 1 cut(s) 1834
PspPPI RGGWCCY 2 cut(s) 432, 838
PstI CTGCAG 7 cut(s) 336, 463, 718, 1306, 2035, 2641, 4496
PstNI CAGNNNCTG 3 cut(s) 841, 2599, 4751
PsuI RGATCY 7 cut(s) 28, 620, 722, 1068, 2099, 2462, 4006
PvuI CGATCG 1 cut(s) 3091
PvuII CAGCTG 3 cut(s) 1232, 1999, 2990
RseI CAYNNNNRTG 5 cut(s) 1925, 2074, 2395, 3710, 4225
SacI GAGCTC 3 cut(s) 2012, 4285, 4860
SapI GCTCTTC 2 cut(s) 1476, 4290
ScaI AGTACT 2 cut(s) 3231, 3453
ScrFI CCNGG 6 cut(s) 126, 1598, 1716, 1784, 1843, 2775
Sfr274I CTCGAG 1 cut(s) 3698
SfuI TTCGAA 1 cut(s) 3038
SinI GGWCC 7 cut(s) 432, 561, 759, 838, 1719, 2771, 4429
SlaI CTCGAG 1 cut(s) 3698
SmiMI CAYNNNNRTG 5 cut(s) 1925, 2074, 2395, 3710, 4225
SnaBI TACGTA 2 cut(s) 2258, 3689
SpeI ACTAGT 3 cut(s) 1211, 3836, 4732
SphI GCATGC 2 cut(s) 1605, 1901
SseBI AGGCCT 3 cut(s) 1589, 1787, 2724
SspI AATATT 3 cut(s) 351, 1975, 2530
SstI GAGCTC 3 cut(s) 2012, 4285, 4860
StuI AGGCCT 3 cut(s) 1589, 1787, 2724
StyD4I CCNGG 6 cut(s) 124, 1596, 1714, 1782, 1841, 2773
StyI CCWWGG 4 cut(s) 1338, 2767, 3238, 3417
TaiI ACGT 9 cut(s) 742, 1152, 2260, 2458, 3227, 3691, 4225, 4447, 4480
TaqI TCGA 9 cut(s) 423, 925, 1347, 3038, 3087, 3119, 3699, 3899, 4131
TaqII GACCGA 3 cut(s) 320, 3858, 4412
TatI WGTACW 6 cut(s) 278, 1195, 3229, 3451, 3682, 4955
TspGWI ACGGA 4 cut(s) 98, 1403, 2634, 3937
Van91I CCANNNNNTGG 2 cut(s) 575, 2831
Vha464I CTTAAG 3 cut(s) 323, 1789, 4633
VneI GTGCAC 1 cut(s) 2052
VpaK11BI GGWCC 7 cut(s) 432, 561, 759, 838, 1719, 2771, 4429
XapI RAATTY 8 cut(s) 1658, 1990, 2239, 2472, 2983, 3481, 3790, 4567
XbaI TCTAGA 1 cut(s) 3932
XceI RCATGY 5 cut(s) 1605, 1901, 2486, 3049, 3632
XhoI CTCGAG 1 cut(s) 3698
XmiI GTMKAC 2 cut(s) 220, 3590
XmnI GAANNNNTTC 1 cut(s) 4059
ZrmI AGTACT 2 cut(s) 3231, 3453
Zsp2I ATGCAT 4 cut(s) 1294, 1522, 2081, 2278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.