AT5G48960

5'-nucleotidase domain-containing protein 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007993
Physical Location & Seq
Forward (+)
19849437 .. 19853598
4162 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G48960.1

Sequence Viewer

Length: 1929 bp
ATGCCTAATTTCTCAGTTAACGTTCCCCAACTCTCATCTCTTTACAGTACAAAAACGCCCAAAGTGAGAATGAATCTATGTGCCGATCAGGTGTTCGATAAAAAGCTTCTGTGGAGAGATATGTCAACGAAGATGAAATTTCCTTCTTTTTCTGCTGCGGAATTACCTGATTTGAGGAAAAGTAACAAGAGGAGGGGATCTCTTAGGATGATCAAGTGCAGAGCCGCCGGAGCTGACGGTGGACGCGTGGCTGTTGGGGATGATGTGTTTTCGGTTACTACTTCTTCTAAGTATGAAGTTGACTATCTGGGTCAAAGTACTAAGGGAGATTTGAATCTCAAGCTTGACCCTCTTCAGTCATTTGGAGATGGGCAGGCTACATTGGAGGGTCCCATTGAGGAGGTAGCGAGAACAGAGGCTCAAGCGGCTGAAAATTTGATTAGAGAGTTGGGTATCCAAGGCCCTTTCTCTGCACAGCACTCTCCTCGGGGTATATTTTGTAGTCGTACATTGAATCTTCGGTCCATTAGTGCAATTGGATATGATATGGATTACACTTTGATGCACTACAATGTCATGGCTTGGGAAGGAAAGGCTTATGACTATTGCATGGAAAATCTAAAGAGCATGGGTTTCCCTGTTGATGGACTTGCTTTTGATCCGGAACTGGTTATCAGGGGTCTCATGATTGACAAAGAGAAAGGTAATTTAGTTAAGGCCGATAGATTTGGGTATGTGAAGAGAGCCATGCACGGTACAAAGATGTTATCAAATAAAGCTGTCAGTGAGATCTATGGAAGGGAGTTAGTTGACCTGCGGAACCAGAGTCGATGGGAGTTTCTCAATACATTTTTTTCAGTTTCAGAGGCTCTGGCTTATGCACAGATGGTTGATAGATTGGATGATGGATTTATTTCGGCAGATCTTGGCACTCTTGATTATAAAGGACTGTATAAGGCTGTTGCAAAAGCTCTCTTCAGAGCACATGTTGAAGGACAACTTAAGAGTGAGATAATGTCCAAGCCGGAACTATTTGTCGAGCCAGACCCAGAACTACCTTTAGCTCTTTTAGATCAAAAGGAGGCTGGTAAGAAGCTCTTGCTTATCACAAACTCGGATTATCACTACACAGACAAAATGATGAAGCATTCATTTAACAAATTCCTTCCCAATGACATGGACTGGCGAGATCTTTTTGACATGGTGATAGTTTCTGCGAGGAAACCAGAGTTCTTCCAGATGTCGCACCCTCTATATGAGGTTGTGACTGGAGAGGGTTTGATGCGTCCATGCTTCAAGGCTGAAACAGGAGGTTTGTACTCAGGAGGAAGTGCTCAAATGATAGAGAGTTCACTCAACGTTCATGGAGATGAGATTTTGTATGTTGGTGACCACATCTACACTGATGTCAGCGTATCCAAAGTCCATCTCAGGTGGCGAACTGCGCTGATTTGCCGTGAACTGGAAGAAGAGTATATGGCTCTAATTGGCAGTCGTGGTCACCGAGAAGAGCTAATAGAGCTTATAAATCAAAAAGAGGTTGTTGGGGATCTCTTTAACCAACTTCGGCTTGCTCTTCAAAGACGAAGCAAAGGCCGTCCTGCTCAGACTCTCGCTGCTACCAACTTGGATGATCAAGAACTGACAGAGACCATGCAAAAGCTTCTTATTGTAATGCAAAGACTAGATGACAAGATTGGTCTAATGCTGGAAACAGATGGCGAGCTCTTTAACAAAAGGTGGGGCTTCCTCTCACGCGCGGGTTTGTGGGATAAAAGCCACTTGATGAGACAAATCGAAAAGTATGCGGATATATACACATCAAGAGTCTCCAACTTCCTCAACTACACACCCTTCATGTATTTCCGCTCACAAGAGCAGTCACTGGCTCACGATTCTCCGCTTCCAGATGCGGGTATAGAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

642

Amino Acids

72.89

Weight (kDa)

5.99

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 165 - 633 7.6e-148 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 942, 1526
Acc36I ACCTGC 1 cut(s) 822
AccBSI CCGCTC 1 cut(s) 1869
AccII CGCG 3 cut(s) 246, 1758, 1760
AccIII TCCGGA 1 cut(s) 661
AciI CCGC 9 cut(s) 158, 225, 425, 817, 1760, 1808, 1867, 1901, 1913
AclI AACGTT 2 cut(s) 21, 1359
AclWI GGATC 3 cut(s) 205, 653, 1557
AcsI RAATTY 3 cut(s) 137, 433, 1160
AcuI CTGAAG 2 cut(s) 338, 961
AfaI GTAC 5 cut(s) 49, 319, 508, 757, 1319
AfiI CCNNNNNNNGG 3 cut(s) 644, 1462, 1913
AflII CTTAAG 1 cut(s) 1001
AflIII ACRYGT 2 cut(s) 244, 985
AgsI TTSAA 5 cut(s) 334, 514, 992, 1297, 1580
Alw21I GWGCWC 3 cut(s) 985, 1336, 1728
Alw26I GTCTC 4 cut(s) 686, 1643, 1783, 1834
AlwI GGATC 3 cut(s) 205, 653, 1557
AlwNI CAGNNNCTG 1 cut(s) 1885
Ama87I CYCGRG 1 cut(s) 486
Aor13HI TCCGGA 1 cut(s) 661
AoxI GGCC 3 cut(s) 460, 717, 1594
ApeKI GCWGC 2 cut(s) 155, 1616
ApoI RAATTY 3 cut(s) 137, 433, 1160
AspLEI GCGC 2 cut(s) 1447, 1760
AspS9I GGNCC 3 cut(s) 389, 461, 522
AsuHPI GGTGA 3 cut(s) 1216, 1400, 1493
AvaI CYCGRG 1 cut(s) 486
AvaII GGWCC 2 cut(s) 389, 522
BanII GRGCYC 1 cut(s) 1728
Bbv12I GWGCWC 3 cut(s) 985, 1336, 1728
BbvI GCAGC 2 cut(s) 142, 1603
BccI CCATC 7 cut(s) 362, 638, 825, 880, 899, 1434, 1712
BceAI ACGGC 2 cut(s) 1440, 1581
BcgI CGANNNNNNTGC 4 cut(s) 467, 501, 1787, 1821
BciVI GTATCC 2 cut(s) 464, 1426
BclI TGATCA 2 cut(s) 210, 1633
BcoDI GTCTC 4 cut(s) 686, 1643, 1783, 1834
BfaI CTAG 2 cut(s) 1685, 1927
BfrI CTTAAG 1 cut(s) 1001
BfuAI ACCTGC 1 cut(s) 822
BfuI GTATCC 2 cut(s) 464, 1426
BglII AGATCT 3 cut(s) 789, 922, 1189
BisI GCNGC 4 cut(s) 156, 225, 426, 1617
BlsI GCNGC 4 cut(s) 157, 226, 427, 1618
BmcAI AGTACT 1 cut(s) 319
Bme18I GGWCC 2 cut(s) 389, 522
BmeT110I CYCGRG 1 cut(s) 486
BmgT120I GGNCC 3 cut(s) 389, 461, 522
BmiI GGNNCC 3 cut(s) 390, 391, 821
BmsI GCATC 3 cut(s) 552, 1272, 1900
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BpmI CTGGAG 1 cut(s) 1290
BpuEI CTTGAG 2 cut(s) 323, 405
BsaBI GATNNNNATC 1 cut(s) 333
BsaI GGTCTC 2 cut(s) 686, 1643
BsaJI CCNNGG 2 cut(s) 457, 485
BsaWI WCCGGW 1 cut(s) 661
Bsc4I CCNNNNNNNGG 3 cut(s) 644, 1462, 1913
Bse1I ACTGG 5 cut(s) 672, 1187, 1273, 1467, 1890
Bse8I GATNNNNATC 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 661
BseDI CCNNGG 2 cut(s) 457, 485
BseGI GGATG 4 cut(s) 213, 265, 907, 1636
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 3 cut(s) 644, 1462, 1913
BseMII CTCAG 4 cut(s) 27, 1335, 1444, 1619
BseNI ACTGG 5 cut(s) 672, 1187, 1273, 1467, 1890
BseRI GAGGAG 3 cut(s) 205, 413, 474
BseXI GCAGC 2 cut(s) 142, 1603
BsgI GTGCAG 2 cut(s) 238, 456
Bsh1236I CGCG 3 cut(s) 246, 1758, 1760
BshFI GGCC 3 cut(s) 462, 719, 1596
BsiHKAI GWGCWC 3 cut(s) 985, 1336, 1728
BsiHKCI CYCGRG 1 cut(s) 486
BsiSI CCGG 3 cut(s) 228, 662, 1025
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 3 cut(s) 644, 1462, 1913
BsmAI GTCTC 4 cut(s) 686, 1643, 1783, 1834
BsmFI GGGAC 1 cut(s) 375
BsmI GAATGC 1 cut(s) 1147
BsnI GGCC 3 cut(s) 462, 719, 1596
Bso31I GGTCTC 2 cut(s) 686, 1643
BsoBI CYCGRG 1 cut(s) 486
Bsp1286I GDGCHC 3 cut(s) 985, 1336, 1728
Bsp13I TCCGGA 1 cut(s) 661
BspACI CCGC 9 cut(s) 158, 225, 425, 817, 1760, 1808, 1867, 1901, 1913
BspANI GGCC 3 cut(s) 462, 719, 1596
BspCNI CTCAG 4 cut(s) 26, 1334, 1443, 1618
BspEI TCCGGA 1 cut(s) 661
BspFNI CGCG 3 cut(s) 246, 1758, 1760
BspHI TCATGA 1 cut(s) 684
BspLI GGNNCC 3 cut(s) 390, 391, 821
BspMI ACCTGC 1 cut(s) 822
BspPI GGATC 3 cut(s) 205, 653, 1557
BspQI GCTCTTC 2 cut(s) 1503, 1581
BspTI CTTAAG 1 cut(s) 1001
BspTNI GGTCTC 2 cut(s) 686, 1643
BsrBI CCGCTC 1 cut(s) 1869
BsrI ACTGG 5 cut(s) 672, 1187, 1273, 1467, 1890
BssECI CCNNGG 2 cut(s) 457, 485
BssT1I CCWWGG 1 cut(s) 457
Bst4CI ACNGT 4 cut(s) 47, 239, 755, 951
Bst6I CTCTTC 6 cut(s) 357, 734, 980, 1464, 1503, 1581
BstAFI CTTAAG 1 cut(s) 1001
BstC8I GCNNGC 3 cut(s) 375, 1572, 1724
BstDEI CTNAG 7 cut(s) 13, 203, 288, 321, 1321, 1430, 1605
BstEII GGTNACC 2 cut(s) 1388, 1499
BstF5I GGATG 4 cut(s) 213, 265, 907, 1636
BstFNI CGCG 3 cut(s) 246, 1758, 1760
BstHHI GCGC 2 cut(s) 1447, 1760
BstMAI GTCTC 4 cut(s) 686, 1643, 1783, 1834
BstMWI GCNNNNNNNGC 4 cut(s) 230, 425, 1444, 1519
BstNSI RCATGY 1 cut(s) 989
BstPI GGTNACC 2 cut(s) 1388, 1499
BstUI CGCG 3 cut(s) 246, 1758, 1760
BstV1I GCAGC 2 cut(s) 142, 1603
BstX2I RGATCY 5 cut(s) 197, 789, 922, 1189, 1549
BstXI CCANNNNNNTGG 1 cut(s) 1177
BstYI RGATCY 5 cut(s) 197, 789, 922, 1189, 1549
BsuI GTATCC 2 cut(s) 464, 1426
BsuRI GGCC 3 cut(s) 462, 719, 1596
BtsCI GGATG 4 cut(s) 213, 265, 907, 1636
BtsIMutI CAGTG 3 cut(s) 790, 1401, 1883
BveI ACCTGC 1 cut(s) 822
Cac8I GCNNGC 3 cut(s) 375, 1572, 1724
CaiI CAGNNNCTG 1 cut(s) 1885
CciI TCATGA 1 cut(s) 684
CfoI GCGC 2 cut(s) 1447, 1760
Cfr13I GGNCC 3 cut(s) 389, 461, 522
CseI GACGC 2 cut(s) 252, 1274
Csp6I GTAC 5 cut(s) 48, 318, 507, 756, 1318
CviQI GTAC 5 cut(s) 48, 318, 507, 756, 1318
DdeI CTNAG 7 cut(s) 13, 203, 288, 321, 1321, 1430, 1605
Eam1104I CTCTTC 6 cut(s) 357, 734, 980, 1464, 1503, 1581
EarI CTCTTC 6 cut(s) 357, 734, 980, 1464, 1503, 1581
Ecl136II GAGCTC 1 cut(s) 1726
Eco130I CCWWGG 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 1728
Eco31I GGTCTC 2 cut(s) 686, 1643
Eco47I GGWCC 2 cut(s) 389, 522
Eco53kI GAGCTC 1 cut(s) 1726
Eco57I CTGAAG 2 cut(s) 338, 961
Eco88I CYCGRG 1 cut(s) 486
Eco91I GGTNACC 2 cut(s) 1388, 1499
EcoICRI GAGCTC 1 cut(s) 1726
EcoO109I RGGNCCY 2 cut(s) 389, 461
EcoO65I GGTNACC 2 cut(s) 1388, 1499
EcoT14I CCWWGG 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 1728
ErhI CCWWGG 1 cut(s) 457
FalI AAGNNNNNCTT 4 cut(s) 984, 1016, 1082, 1114
FaqI GGGAC 1 cut(s) 375
FauI CCCGC 2 cut(s) 1753, 1906
FbaI TGATCA 2 cut(s) 210, 1633
Fnu4HI GCNGC 4 cut(s) 156, 225, 426, 1617
FokI GGATG 4 cut(s) 220, 272, 914, 1643
FriOI GRGCYC 1 cut(s) 1728
Fsp4HI GCNGC 4 cut(s) 156, 225, 426, 1617
FspBI CTAG 2 cut(s) 1685, 1927
GlaI GCGC 2 cut(s) 1446, 1759
GluI GCNGC 4 cut(s) 156, 225, 426, 1617
GsuI CTGGAG 1 cut(s) 1290
HaeIII GGCC 3 cut(s) 462, 719, 1596
HapII CCGG 3 cut(s) 228, 662, 1025
HgaI GACGC 2 cut(s) 252, 1274
HhaI GCGC 2 cut(s) 1447, 1760
Hin6I GCGC 2 cut(s) 1445, 1758
HinP1I GCGC 2 cut(s) 1445, 1758
HincII GTYRAC 4 cut(s) 19, 126, 301, 811
HindII GTYRAC 4 cut(s) 19, 126, 301, 811
HindIII AAGCTT 3 cut(s) 104, 341, 1661
HinfI GANTC 7 cut(s) 73, 334, 514, 826, 1609, 1827, 1895
HpaI GTTAAC 1 cut(s) 19
HpaII CCGG 3 cut(s) 228, 662, 1025
HphI GGTGA 3 cut(s) 1216, 1400, 1493
Hpy166II GTNNAC 7 cut(s) 19, 126, 242, 301, 811, 1352, 1460
Hpy188I TCNGA 4 cut(s) 865, 980, 1117, 1608
Hpy188III TCNNGA 9 cut(s) 662, 685, 935, 1237, 1323, 1637, 1824, 1892, 1907
Hpy8I GTNNAC 7 cut(s) 19, 126, 242, 301, 811, 1352, 1460
HpyAV CCTTC 6 cut(s) 153, 581, 792, 986, 1175, 1864
HpyCH4III ACNGT 4 cut(s) 47, 239, 755, 951
HpyCH4IV ACGT 2 cut(s) 21, 1359
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 425, 1444, 1519
HpyF3I CTNAG 7 cut(s) 13, 203, 288, 321, 1321, 1430, 1605
HpySE526I ACGT 2 cut(s) 21, 1359
HspAI GCGC 2 cut(s) 1445, 1758
KflI GGGWCCC 1 cut(s) 389
Kpn2I TCCGGA 1 cut(s) 661
Ksp22I TGATCA 2 cut(s) 210, 1633
KspAI GTTAAC 1 cut(s) 19
LguI GCTCTTC 2 cut(s) 1503, 1581
LmnI GCTCC 1 cut(s) 230
Lsp1109I GCAGC 2 cut(s) 142, 1603
LweI GCATC 3 cut(s) 552, 1272, 1900
MaeI CTAG 2 cut(s) 1685, 1927
MaeII ACGT 2 cut(s) 21, 1359
MaeIII GTNAC 6 cut(s) 182, 274, 1264, 1388, 1499, 1881
MbiI CCGCTC 1 cut(s) 1869
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 5 cut(s) 197, 789, 922, 1189, 1549
MhlI GDGCHC 3 cut(s) 985, 1336, 1728
MluCI AATT 8 cut(s) 7, 137, 161, 433, 534, 706, 1160, 1485
MluI ACGCGT 1 cut(s) 244
MlyI GAGTC 3 cut(s) 835, 1603, 1836
MmeI TCCRAC 1 cut(s) 1857
MroI TCCGGA 1 cut(s) 661
MseI TTAA 6 cut(s) 18, 714, 1002, 1155, 1557, 1731
MslI CAYNNNNRTG 3 cut(s) 560, 570, 1368
MspCI CTTAAG 1 cut(s) 1001
MspI CCGG 3 cut(s) 228, 662, 1025
MunI CAATTG 1 cut(s) 534
Mva1269I GAATGC 1 cut(s) 1147
MvnI CGCG 3 cut(s) 246, 1758, 1760
MwoI GCNNNNNNNGC 4 cut(s) 230, 425, 1444, 1519
NlaIV GGNNCC 3 cut(s) 390, 391, 821
NmuCI GTSAC 4 cut(s) 1264, 1388, 1499, 1881
NspI RCATGY 1 cut(s) 989
PagI TCATGA 1 cut(s) 684
PciI ACATGT 1 cut(s) 985
PciSI GCTCTTC 2 cut(s) 1503, 1581
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 1501
PctI GAATGC 1 cut(s) 1147
PfeI GAWTC 4 cut(s) 73, 334, 514, 1895
PkrI GCNGC 4 cut(s) 157, 226, 427, 1618
PleI GAGTC 3 cut(s) 834, 1603, 1835
PpsI GAGTC 3 cut(s) 834, 1603, 1835
PpuMI RGGWCCY 1 cut(s) 389
PscI ACATGT 1 cut(s) 985
PsiI TTATAA 2 cut(s) 942, 1526
Psp124BI GAGCTC 1 cut(s) 1728
Psp1406I AACGTT 2 cut(s) 21, 1359
Psp5II RGGWCCY 1 cut(s) 389
PspEI GGTNACC 2 cut(s) 1388, 1499
PspN4I GGNNCC 3 cut(s) 390, 391, 821
PspPI GGNCC 3 cut(s) 389, 461, 522
PspPPI RGGWCCY 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 1885
PsuI RGATCY 5 cut(s) 197, 789, 922, 1189, 1549
RsaI GTAC 5 cut(s) 49, 319, 508, 757, 1319
RsaNI GTAC 5 cut(s) 48, 318, 507, 756, 1318
RseI CAYNNNNRTG 3 cut(s) 560, 570, 1368
SacI GAGCTC 1 cut(s) 1728
SapI GCTCTTC 2 cut(s) 1503, 1581
SaqAI TTAA 6 cut(s) 18, 714, 1002, 1155, 1557, 1731
SatI GCNGC 4 cut(s) 156, 225, 426, 1617
Sau96I GGNCC 3 cut(s) 389, 461, 522
ScaI AGTACT 1 cut(s) 319
SchI GAGTC 3 cut(s) 835, 1603, 1836
SduI GDGCHC 3 cut(s) 985, 1336, 1728
SfaNI GCATC 3 cut(s) 552, 1272, 1900
SinI GGWCC 2 cut(s) 389, 522
SmiMI CAYNNNNRTG 3 cut(s) 560, 570, 1368
SmlI CTYRAG 3 cut(s) 338, 420, 1001
SmoI CTYRAG 3 cut(s) 338, 420, 1001
Sse9I AATT 8 cut(s) 7, 137, 161, 433, 534, 706, 1160, 1485
SsiI CCGC 9 cut(s) 158, 225, 425, 817, 1760, 1808, 1867, 1901, 1913
SspMI CTAG 2 cut(s) 1685, 1927
SstI GAGCTC 1 cut(s) 1728
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 4 cut(s) 47, 239, 755, 951
TaiI ACGT 2 cut(s) 24, 1362
TaqI TCGA 4 cut(s) 96, 829, 1038, 1797
TaqII GACCGA 1 cut(s) 510
TasI AATT 8 cut(s) 7, 137, 161, 433, 534, 706, 1160, 1485
TatI WGTACW 3 cut(s) 47, 317, 1317
TauI GCSGC 2 cut(s) 227, 428
TfiI GAWTC 4 cut(s) 73, 334, 514, 1895
Tru1I TTAA 6 cut(s) 18, 714, 1002, 1155, 1557, 1731
Tru9I TTAA 6 cut(s) 18, 714, 1002, 1155, 1557, 1731
TscAI CASTG 3 cut(s) 790, 1408, 1890
TseFI GTSAC 4 cut(s) 1264, 1388, 1499, 1881
TseI GCWGC 2 cut(s) 155, 1616
Tsp45I GTSAC 4 cut(s) 1264, 1388, 1499, 1881
TspDTI ATGAA 7 cut(s) 86, 149, 309, 1140, 1157, 1352, 1846
TspRI CASTG 3 cut(s) 790, 1408, 1890
Vha464I CTTAAG 1 cut(s) 1001
VpaK11BI GGWCC 2 cut(s) 389, 522
XapI RAATTY 3 cut(s) 137, 433, 1160
XceI RCATGY 1 cut(s) 989
XspI CTAG 2 cut(s) 1685, 1927
ZrmI AGTACT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.