MD16G1135200.v1.1

Cytosolic purine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007993
Physical Location & Seq
Forward (+)
10381634 .. 10387053
5420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1135200.v1.1.491

Sequence Viewer

Length: 1923 bp
ATGTACGCGTTCGAGGCGTTGGGGAAATTCTCAGTTTTCAGAGAGACCCCCGTTTCAATGGCGGCTTCCAGCTCTCTCAGAACCCCGTTTCGAATCCGACCCATGTCCCAGAACTTCATCAGCAGCAAGAATTTGCGCCGGAATCGTGGAATGAAGTGCAACTGCAGCAGCAGCGGCAGCGGCGTTGACGAGAAGTCAGCGTTTTCAGTGACTTCGTCGAGCAAGTATGAAGTGGATTACTTGGGAGAGAAAACCAAAGGAGATTTGAATGTGAAGATGGAGCATCTCCAGGCTTTTGGAATCGATGGTGATGTAACTTTGAAAGGTCCGATTGAGGAAGTTGCCAGAGTGGAGGCTGAAGAAGCTGGAGACTTGCTCAGAGACTTGGGTATTCCGAGCCCTTTTTCGTCGAGGCAATCACCTCGTGGCATTTTCTGTAGCCGCACATTGAATCTCCGATCAATCAGTGCCATCGGATATGATATGGACTACACCTTGATGCATTATAACGTGATTTGTTGGAGCCAGAGTACATTAGCTTGGGAAGGGAGGGCTTATGATTACTGTATGGAAAATTTAAAGAAAGTGGGATTCCCTGTTGACGGTCTTGCATTTGACCCTGACTTGGTTATTAGAGGCCTCGTCATAGACAAAGAGAACGGAAACTTGGTGAAGGCTGATCGATTTGGTTATGTTAAGAGGGCTATGCATGGCACATCCATGCTATCTAATCGAGCTGTAAGTGAGATGTACGGGAGGGAACTGGTGGACCTCCGGAAGGAAACTCGGTGGGAATTCCTTAATACACTGTTCTCTGTCTCAGAAGCTGTGGCTTATATGCAGATGGTTGACAGATTGGATGATGGAACCATACCCGCAGAACTTGGTCCACTTGATTATAAAGGGCTCTATAAGGCTGTTGGAACAGCCCTCTTCTGGGCACATGTCGAGGGTCAACTAAAGAGCGAGATAATGTCTAAGCCTGAACTGTTTGTGACGCCTGATCCAGAATTGCCACTAGCGCTTTTGGATCAAAAAGAGGCTGGTAAAAAGCTTCTGCTCATTACCAACTCAGATTATCATTACACCGACAAAATGATGCAGCATTCCTTCAACAGATTTCTTCCCAATGAGATGGGTTGGAGGGATCTATTTGACATTGTAATAGTCTCTGCAAGGAAGCCAGAGTTCTTCCAAATGTCACACCCAATGTATGAGGTAGTGACGGGTGAGGGCCTCATGCGTCCATGCTTCAAGGCTAAAACAGGGGGATTGTATTCAGGAGGTAGTGCTCAGATGGTTGAGAATTCCCTAAATATCCACGGAGATGAGATACTATATGTTGGTGATCATATCTACACCGATGTAAGTCAATCCAAAGTTCATCTGCGATGGCGAACAGCATTGATTTGTCGAGAATTGGAAGAAGAGTATAGTGCTTTGATTCATAGCCGGGGTCATAGAGCAGCACTAGTAGATCTTATAAATCAAAAGGAGGTCGTAGGGGATCTTTTCAACCAACTTCGGCTCGCCTCACAAAGGCGAACTAAAGGGCGTCCTGCTCAAACCCTTGCTGCAACAAACTTGGATGATCAAGAACTCTCAGAAAGCATGCAAAAGCTACTTATTGTTATGCAACGACTAGACCAGAAAATTGCTCCGATGCTAGAAGCAGATGGAGAGCTCTTCAACAAAAGGTGGGGATTTCTTTCACGTGCAGGGTTCTGGGATAAAAGCCATTTGATGAGGCAAATCGAGAAGTATGCTGATATATATACCTCTAGGGTGTCCAATTTCCTACATTATACACCGTTCATGTATTTCCGCTCGCAGGAACAGACGCTTGCTCATGATTCGTATTCATACTACTGTTCGAGGTTTAATGGATCTGCCATGGACGACGAGGTGAACTGTATGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

641

Amino Acids

72.78

Weight (kDa)

6.16

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 144 - 619 8.6e-144 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 507, 900, 1484
AccBSI CCGCTC 1 cut(s) 1827
AccII CGCG 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 774
AciI CCGC 6 cut(s) 62, 174, 180, 442, 876, 1825
AclWI GGATC 5 cut(s) 998, 1038, 1155, 1515, 1894
AcsI RAATTY 5 cut(s) 26, 130, 574, 794, 1306
AcuI CTGAAG 1 cut(s) 378
AcvI CACGTG 1 cut(s) 1715
AcyI GRCGYC 2 cut(s) 998, 1555
AdeI CACNNNGTG 1 cut(s) 425
AfaI GTAC 3 cut(s) 5, 532, 752
AfeI AGCGCT 1 cut(s) 1023
AfiI CCNNNNNNNGG 7 cut(s) 602, 625, 778, 936, 937, 1539, 1831
AflIII ACRYGT 2 cut(s) 6, 943
AgsI TTSAA 8 cut(s) 57, 268, 322, 451, 1114, 1255, 1516, 1690
AhdI GACNNNNNGTC 1 cut(s) 193
AhlI ACTAGT 1 cut(s) 1471
AjnI CCWGG 1 cut(s) 288
AjuI GAANNNNNNNTTGG 2 cut(s) 650, 682
AluBI AGCT 8 cut(s) 72, 365, 539, 737, 827, 1054, 1621, 1684
AluI AGCT 8 cut(s) 72, 365, 539, 737, 827, 1054, 1621, 1684
Alw21I GWGCWC 2 cut(s) 1294, 1686
Alw26I GTCTC 5 cut(s) 38, 363, 375, 823, 1174
AlwI GGATC 5 cut(s) 998, 1038, 1155, 1515, 1894
AlwNI CAGNNNCTG 1 cut(s) 827
Aor13HI TCCGGA 1 cut(s) 774
Aor51HI AGCGCT 1 cut(s) 1023
AoxI GGCC 2 cut(s) 637, 1234
ApeKI GCWGC 8 cut(s) 123, 165, 168, 171, 177, 1102, 1466, 1574
ApoI RAATTY 5 cut(s) 26, 130, 574, 794, 1306
AspLEI GCGC 2 cut(s) 138, 1024
AspS9I GGNCC 4 cut(s) 326, 769, 887, 1234
AsuC2I CCSGG 1 cut(s) 1454
AsuHPI GGTGA 6 cut(s) 320, 411, 682, 1241, 1358, 1918
AsuII TTCGAA 1 cut(s) 91
AvaII GGWCC 3 cut(s) 326, 769, 887
BaeGI GKGCMC 1 cut(s) 943
BanII GRGCYC 3 cut(s) 401, 909, 1686
BauI CACGAG 1 cut(s) 423
BbrPI CACGTG 1 cut(s) 1715
Bbv12I GWGCWC 2 cut(s) 1294, 1686
BbvI GCAGC 8 cut(s) 135, 177, 180, 183, 189, 1114, 1478, 1561
BccI CCATC 9 cut(s) 271, 299, 479, 838, 857, 1129, 1291, 1386, 1670
BcgI CGANNNNNNTGC 4 cut(s) 404, 438, 1745, 1779
BciT130I CCWGG 1 cut(s) 290
BclI TGATCA 2 cut(s) 1348, 1591
BcnI CCSGG 1 cut(s) 1454
BcoDI GTCTC 5 cut(s) 38, 363, 375, 823, 1174
BcuI ACTAGT 1 cut(s) 1471
BfaI CTAG 5 cut(s) 1019, 1472, 1643, 1667, 1782
BfmI CTRYAG 2 cut(s) 163, 436
BfoI RGCGCY 1 cut(s) 1025
BglII AGATCT 1 cut(s) 1477
Bme1390I CCNGG 2 cut(s) 290, 1454
Bme18I GGWCC 3 cut(s) 326, 769, 887
BmeRI GACNNNNNGTC 1 cut(s) 193
BmgT120I GGNCC 4 cut(s) 326, 769, 887, 1234
BmiI GGNNCC 2 cut(s) 524, 868
BmrFI CCNGG 2 cut(s) 290, 1454
BmsI GCATC 4 cut(s) 292, 489, 1089, 1653
BoxI GACNNNNGTC 1 cut(s) 103
BplI GAGNNNNNCTC 2 cut(s) 360, 392
BpmI CTGGAG 2 cut(s) 272, 387
Bpu14I TTCGAA 1 cut(s) 91
BpuMI CCSGG 1 cut(s) 1454
Bsa29I ATCGAT 2 cut(s) 303, 682
BsaAI YACGTR 1 cut(s) 1715
BsaHI GRCGYC 2 cut(s) 998, 1555
BsaI GGTCTC 1 cut(s) 38
BsaJI CCNNGG 3 cut(s) 1321, 1453, 1893
BsaWI WCCGGW 1 cut(s) 774
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 7 cut(s) 602, 625, 778, 936, 937, 1539, 1831
Bse1I ACTGG 1 cut(s) 768
BseAI TCCGGA 1 cut(s) 774
BseBI CCWGG 1 cut(s) 290
BseCI ATCGAT 2 cut(s) 303, 682
BseDI CCNNGG 3 cut(s) 1321, 1453, 1893
BseGI GGATG 3 cut(s) 716, 865, 1594
BseLI CCNNNNNNNGG 7 cut(s) 602, 625, 778, 936, 937, 1539, 1831
BseMII CTCAG 7 cut(s) 45, 91, 391, 834, 1086, 1307, 1617
BseNI ACTGG 1 cut(s) 768
BseSI GKGCMC 1 cut(s) 943
BseXI GCAGC 8 cut(s) 135, 177, 180, 183, 189, 1114, 1478, 1561
BsgI GTGCAG 1 cut(s) 1737
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 2 cut(s) 639, 1236
BshVI ATCGAT 2 cut(s) 303, 682
BsiHKAI GWGCWC 2 cut(s) 1294, 1686
BsiSI CCGG 3 cut(s) 139, 775, 1453
BslFI GGGAC 1 cut(s) 91
BslI CCNNNNNNNGG 7 cut(s) 602, 625, 778, 936, 937, 1539, 1831
BsmAI GTCTC 5 cut(s) 38, 363, 375, 823, 1174
BsmFI GGGAC 1 cut(s) 91
BsmI GAATGC 1 cut(s) 1105
BsnI GGCC 2 cut(s) 639, 1236
Bso31I GGTCTC 1 cut(s) 38
Bsp119I TTCGAA 1 cut(s) 91
Bsp1286I GDGCHC 5 cut(s) 401, 909, 943, 1294, 1686
Bsp13I TCCGGA 1 cut(s) 774
Bsp19I CCATGG 1 cut(s) 1893
BspACI CCGC 6 cut(s) 62, 174, 180, 442, 876, 1825
BspANI GGCC 2 cut(s) 639, 1236
BspCNI CTCAG 7 cut(s) 44, 90, 390, 833, 1085, 1306, 1616
BspDI ATCGAT 2 cut(s) 303, 682
BspEI TCCGGA 1 cut(s) 774
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 1849
BspLI GGNNCC 2 cut(s) 524, 868
BspMAI CTGCAG 1 cut(s) 167
BspPI GGATC 5 cut(s) 998, 1038, 1155, 1515, 1894
BspQI GCTCTTC 1 cut(s) 1691
BspT104I TTCGAA 1 cut(s) 91
BspTNI GGTCTC 1 cut(s) 38
BsrBI CCGCTC 1 cut(s) 1827
BsrI ACTGG 1 cut(s) 768
BssECI CCNNGG 3 cut(s) 1321, 1453, 1893
BssNI GRCGYC 2 cut(s) 998, 1555
BssSI CACGAG 1 cut(s) 423
BssT1I CCWWGG 1 cut(s) 1893
Bst2BI CACGAG 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 290
Bst4CI ACNGT 7 cut(s) 566, 605, 810, 990, 1812, 1871, 1913
Bst6I CTCTTC 3 cut(s) 938, 1422, 1691
BstACI GRCGYC 2 cut(s) 998, 1555
BstBAI YACGTR 1 cut(s) 1715
BstBI TTCGAA 1 cut(s) 91
BstC8I GCNNGC 4 cut(s) 1530, 1613, 1829, 1845
BstDEI CTNAG 8 cut(s) 31, 77, 377, 820, 978, 1072, 1293, 1603
BstDSI CCRYGG 2 cut(s) 1321, 1893
BstENI CCTNNNNNAGG 2 cut(s) 776, 1537
BstF5I GGATG 3 cut(s) 716, 865, 1594
BstFNI CGCG 1 cut(s) 8
BstH2I RGCGCY 1 cut(s) 1025
BstHHI GCGC 2 cut(s) 138, 1024
BstMAI GTCTC 5 cut(s) 38, 363, 375, 823, 1174
BstMWI GCNNNNNNNGC 8 cut(s) 14, 165, 171, 174, 177, 180, 362, 1021
BstNI CCWGG 1 cut(s) 290
BstNSI RCATGY 2 cut(s) 947, 1615
BstPAI GACNNNNGTC 1 cut(s) 103
BstSCI CCNGG 2 cut(s) 288, 1452
BstSFI CTRYAG 2 cut(s) 163, 436
BstSLI GKGCMC 1 cut(s) 943
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 8 cut(s) 135, 177, 180, 183, 189, 1114, 1478, 1561
BstX2I RGATCY 4 cut(s) 1147, 1477, 1507, 1886
BstXI CCANNNNNNTGG 2 cut(s) 296, 1135
BstYI RGATCY 4 cut(s) 1147, 1477, 1507, 1886
Bsu15I ATCGAT 2 cut(s) 303, 682
BsuRI GGCC 2 cut(s) 639, 1236
BsuTUI ATCGAT 2 cut(s) 303, 682
BtgI CCRYGG 2 cut(s) 1321, 1893
BtgZI GCGATG 1 cut(s) 1405
BtsCI GGATG 3 cut(s) 716, 865, 1594
BtsIMutI CAGTG 3 cut(s) 213, 472, 806
Cac8I GCNNGC 4 cut(s) 1530, 1613, 1829, 1845
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 1849
CfoI GCGC 2 cut(s) 138, 1024
Cfr13I GGNCC 4 cut(s) 326, 769, 887, 1234
ClaI ATCGAT 2 cut(s) 303, 682
CseI GACGC 4 cut(s) 1006, 1232, 1544, 1849
Csp6I GTAC 3 cut(s) 4, 531, 751
CviQI GTAC 3 cut(s) 4, 531, 751
DdeI CTNAG 8 cut(s) 31, 77, 377, 820, 978, 1072, 1293, 1603
DraI TTTAAA 1 cut(s) 579
DraIII CACNNNGTG 1 cut(s) 425
DriI GACNNNNNGTC 1 cut(s) 193
Eam1104I CTCTTC 3 cut(s) 938, 1422, 1691
Eam1105I GACNNNNNGTC 1 cut(s) 193
EarI CTCTTC 3 cut(s) 938, 1422, 1691
Ecl136II GAGCTC 1 cut(s) 1684
Eco130I CCWWGG 1 cut(s) 1893
Eco147I AGGCCT 1 cut(s) 639
Eco24I GRGCYC 3 cut(s) 401, 909, 1686
Eco31I GGTCTC 1 cut(s) 38
Eco47I GGWCC 3 cut(s) 326, 769, 887
Eco47III AGCGCT 1 cut(s) 1023
Eco53kI GAGCTC 1 cut(s) 1684
Eco57I CTGAAG 1 cut(s) 378
Eco72I CACGTG 1 cut(s) 1715
EcoICRI GAGCTC 1 cut(s) 1684
EcoNI CCTNNNNNAGG 2 cut(s) 776, 1537
EcoO109I RGGNCCY 1 cut(s) 1234
EcoRI GAATTC 2 cut(s) 794, 1306
EcoRII CCWGG 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 1893
EcoT22I ATGCAT 2 cut(s) 504, 711
EcoT38I GRGCYC 3 cut(s) 401, 909, 1686
ErhI CCWWGG 1 cut(s) 1893
FaqI GGGAC 1 cut(s) 91
FauI CCCGC 1 cut(s) 883
FbaI TGATCA 2 cut(s) 1348, 1591
FokI GGATG 3 cut(s) 703, 872, 1601
FriOI GRGCYC 3 cut(s) 401, 909, 1686
FspBI CTAG 5 cut(s) 1019, 1472, 1643, 1667, 1782
GlaI GCGC 2 cut(s) 137, 1023
GsuI CTGGAG 2 cut(s) 272, 387
HaeII RGCGCY 1 cut(s) 1025
HaeIII GGCC 2 cut(s) 639, 1236
HapII CCGG 3 cut(s) 139, 775, 1453
HgaI GACGC 4 cut(s) 1006, 1232, 1544, 1849
HhaI GCGC 2 cut(s) 138, 1024
Hin1I GRCGYC 2 cut(s) 998, 1555
Hin6I GCGC 2 cut(s) 136, 1022
HinP1I GCGC 2 cut(s) 136, 1022
HincII GTYRAC 4 cut(s) 187, 601, 850, 956
HindII GTYRAC 4 cut(s) 187, 601, 850, 956
HindIII AAGCTT 1 cut(s) 1052
HinfI GANTC 7 cut(s) 93, 142, 300, 451, 591, 1444, 1853
HpaII CCGG 3 cut(s) 139, 775, 1453
HphI GGTGA 6 cut(s) 320, 411, 682, 1241, 1358, 1918
Hpy166II GTNNAC 7 cut(s) 187, 601, 769, 850, 890, 956, 1909
Hpy188III TCNNGA 7 cut(s) 775, 1007, 1281, 1415, 1595, 1756, 1850
Hpy8I GTNNAC 7 cut(s) 187, 601, 769, 850, 890, 956, 1909
Hpy99I CGWCG 3 cut(s) 220, 412, 1904
HpyAV CCTTC 4 cut(s) 539, 667, 772, 1120
HpyCH4III ACNGT 7 cut(s) 566, 605, 810, 990, 1812, 1871, 1913
HpyCH4IV ACGT 2 cut(s) 510, 1714
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 165, 171, 174, 177, 180, 362, 1021
HpyF3I CTNAG 8 cut(s) 31, 77, 377, 820, 978, 1072, 1293, 1603
HpySE526I ACGT 2 cut(s) 510, 1714
Hsp92I GRCGYC 2 cut(s) 998, 1555
HspAI GCGC 2 cut(s) 136, 1022
Kpn2I TCCGGA 1 cut(s) 774
Ksp22I TGATCA 2 cut(s) 1348, 1591
LguI GCTCTTC 1 cut(s) 1691
LmnI GCTCC 3 cut(s) 280, 522, 1663
Lsp1109I GCAGC 8 cut(s) 135, 177, 180, 183, 189, 1114, 1478, 1561
LweI GCATC 4 cut(s) 292, 489, 1089, 1653
MaeI CTAG 5 cut(s) 1019, 1472, 1643, 1667, 1782
MaeII ACGT 2 cut(s) 510, 1714
MaeIII GTNAC 5 cut(s) 208, 313, 994, 1200, 1222
MbiI CCGCTC 1 cut(s) 1827
MboII GAAGA 8 cut(s) 286, 371, 925, 1115, 1183, 1436, 1439, 1678
MflI RGATCY 4 cut(s) 1147, 1477, 1507, 1886
MhlI GDGCHC 5 cut(s) 401, 909, 943, 1294, 1686
MluCI AATT 9 cut(s) 26, 130, 574, 794, 1010, 1306, 1418, 1653, 1792
MluI ACGCGT 1 cut(s) 6
MmeI TCCRAC 4 cut(s) 121, 500, 901, 1121
Mph1103I ATGCAT 2 cut(s) 504, 711
MroI TCCGGA 1 cut(s) 774
MseI TTAA 4 cut(s) 578, 696, 801, 1881
MslI CAYNNNNRTG 3 cut(s) 497, 719, 1326
MspA1I CMGCKG 2 cut(s) 174, 180
MspI CCGG 3 cut(s) 139, 775, 1453
MspR9I CCNGG 2 cut(s) 290, 1454
Mva1269I GAATGC 1 cut(s) 1105
MvaI CCWGG 1 cut(s) 290
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 8 cut(s) 14, 165, 171, 174, 177, 180, 362, 1021
NciI CCSGG 1 cut(s) 1454
NcoI CCATGG 1 cut(s) 1893
NlaIV GGNNCC 2 cut(s) 524, 868
NmuCI GTSAC 4 cut(s) 208, 994, 1200, 1222
NsiI ATGCAT 2 cut(s) 504, 711
NspI RCATGY 2 cut(s) 947, 1615
NspV TTCGAA 1 cut(s) 91
PaeI GCATGC 1 cut(s) 1615
PagI TCATGA 1 cut(s) 1849
PceI AGGCCT 1 cut(s) 639
PciI ACATGT 1 cut(s) 943
PciSI GCTCTTC 1 cut(s) 1691
PctI GAATGC 1 cut(s) 1105
PfeI GAWTC 7 cut(s) 93, 142, 300, 451, 591, 1444, 1853
PflFI GACNNNGTC 1 cut(s) 214
PmaCI CACGTG 1 cut(s) 1715
PmlI CACGTG 1 cut(s) 1715
Ppu21I YACGTR 1 cut(s) 1715
PscI ACATGT 1 cut(s) 943
PshAI GACNNNNGTC 1 cut(s) 103
PsiI TTATAA 3 cut(s) 507, 900, 1484
Psp124BI GAGCTC 1 cut(s) 1686
Psp6I CCWGG 1 cut(s) 288
PspCI CACGTG 1 cut(s) 1715
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 2 cut(s) 524, 868
PspPI GGNCC 4 cut(s) 326, 769, 887, 1234
PstI CTGCAG 1 cut(s) 167
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 4 cut(s) 1147, 1477, 1507, 1886
PsyI GACNNNGTC 1 cut(s) 214
RsaI GTAC 3 cut(s) 5, 532, 752
RsaNI GTAC 3 cut(s) 4, 531, 751
RseI CAYNNNNRTG 3 cut(s) 497, 719, 1326
SacI GAGCTC 1 cut(s) 1686
SapI GCTCTTC 1 cut(s) 1691
SaqAI TTAA 4 cut(s) 578, 696, 801, 1881
Sau96I GGNCC 4 cut(s) 326, 769, 887, 1234
ScrFI CCNGG 2 cut(s) 290, 1454
SduI GDGCHC 5 cut(s) 401, 909, 943, 1294, 1686
SfaNI GCATC 4 cut(s) 292, 489, 1089, 1653
SfcI CTRYAG 2 cut(s) 163, 436
SfuI TTCGAA 1 cut(s) 91
SinI GGWCC 3 cut(s) 326, 769, 887
SmiMI CAYNNNNRTG 3 cut(s) 497, 719, 1326
SpeI ACTAGT 1 cut(s) 1471
SphI GCATGC 1 cut(s) 1615
Sse9I AATT 9 cut(s) 26, 130, 574, 794, 1010, 1306, 1418, 1653, 1792
SseBI AGGCCT 1 cut(s) 639
SsiI CCGC 6 cut(s) 62, 174, 180, 442, 876, 1825
SspMI CTAG 5 cut(s) 1019, 1472, 1643, 1667, 1782
SstI GAGCTC 1 cut(s) 1686
StuI AGGCCT 1 cut(s) 639
StyD4I CCNGG 2 cut(s) 288, 1452
StyI CCWWGG 1 cut(s) 1893
TaaI ACNGT 7 cut(s) 566, 605, 810, 990, 1812, 1871, 1913
TaiI ACGT 2 cut(s) 513, 1717
TasI AATT 9 cut(s) 26, 130, 574, 794, 1010, 1306, 1418, 1653, 1792
TatI WGTACW 1 cut(s) 530
TauI GCSGC 4 cut(s) 65, 177, 183, 444
TfiI GAWTC 7 cut(s) 93, 142, 300, 451, 591, 1444, 1853
Tru1I TTAA 4 cut(s) 578, 696, 801, 1881
Tru9I TTAA 4 cut(s) 578, 696, 801, 1881
TscAI CASTG 3 cut(s) 213, 472, 813
TseFI GTSAC 4 cut(s) 208, 994, 1200, 1222
TseI GCWGC 8 cut(s) 123, 165, 168, 171, 177, 1102, 1466, 1574
Tsp45I GTSAC 4 cut(s) 208, 994, 1200, 1222
TspDTI ATGAA 7 cut(s) 106, 167, 243, 1373, 1436, 1804, 1851
TspGWI ACGGA 2 cut(s) 675, 1338
TspRI CASTG 3 cut(s) 213, 472, 813
Tth111I GACNNNGTC 1 cut(s) 214
VpaK11BI GGWCC 3 cut(s) 326, 769, 887
XagI CCTNNNNNAGG 2 cut(s) 776, 1537
XapI RAATTY 5 cut(s) 26, 130, 574, 794, 1306
XceI RCATGY 2 cut(s) 947, 1615
XspI CTAG 5 cut(s) 1019, 1472, 1643, 1667, 1782
Zsp2I ATGCAT 2 cut(s) 504, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.