MD13G1140600.v1.1

Cytosolic purine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007993
Physical Location & Seq
Reverse (-)
10841676 .. 10846974
5299 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1140600.v1.1.491

Sequence Viewer

Length: 1902 bp
ATGTACGCGGCCGAGGCGTTGGGGAAGTTCTCGGTTTTCAGAGAGACCCCTGCATCAATGGCGGCCATGAACCATCTCAGAGGTCCGGTTCGAATCCGACCCATGTCCCAGAACTTCATCAGCAGCAAGAACTTGCTCTGGAATCACGGAATGAAGTGCCAGTGCAGCAGCGGCGGCGGCAGCGTGGACGAGAAGTCGGCGTTTTCTGTGACTTCGTCGAGCAAGTACGAAGTGGATTACTTGGGAGAGAAAACCAAAGGGGATTTGAATGTCAAGGTGGAGCATCTCGAAGCTTTTGGAATTGATGGTGATGTAACTTTGAAAGGTCCCATTGAGGAAGTTGCCAGAGTGGAGGCTGAAGAAGCTGGAGACTTGCTCAGAGACTTGGGCATTCCGAGCCCTTTTTCGTCAAGGCAATCGCCTCGTGGCATTTTTTGTAGCCGCACATTGAATCTTCGATCAATCAGTGCCATTGGATATGATATGGACTACACATTGATGCATTATAATGTGATTGCTTGGGAAGGGAGAGCTTATGATTACTGTATGGAGAATTTGAAGAAAGTGGGCTTCCCCGTTGACGGTCTTGCATTTGACCCCGACTTGGTTATTAGAGGCCTTGTCATAGACAAAGAGAAAGGAAACTTGGTGAAGCCTGATCGATTTGGTTATGTTAAAAGGGCTATGCATGGCACAACCATGCTATCTAATCGAGCTGTGAGTGAGATGTACGGGAGGGAACTGGTGGACCTCCGGAAGGAAACTCGATGGGAGTTCCTTAATACACTGTTCTCTGTCTCAGAAGCTGTGGCTTATATGCAGATGGTTGACAGATTGGATGATGGAACCATAGCCGCAGAAATTGGTCCACTTGATTATAAAGGGCTCTATAAGGCTGTTGGAAGAGCCCTCTTCCGGGCACATGTCGAGGGTCAACTAAAGAGCGAAATAATGTCGAAGCCTGAACTGTTTGTGGAGCCTGATCCAGAATTGCCATTAGCGCTTTTGGATCAAAAAGAGGCTGGTAAAAAGCTTCTGCTCATTACCAACTCAGATTATCATTACACAAACAAAATGATGCAGCATTCCTTCAACAGATTTCTTCCCAATGAGATGGGTTGGAGGGATCTATTTGACATTGTAATAGTCTCTGCAAGGAAGCCAGAGTTCTTCCAAATGTCACACCCAATGTATGAGGTAGTGACGGGTGAGGGCCTCATGCGTCCATGCTTCAAGGCTAAAACAGGGGGCTTGTATTCAGGGGGAAGTGCTCAGATGGTTGAGAATTCCCTAAATATCCATGGAGATGAAATACTGTATGTTGGTGATCATATCTACACAGATGTAAGTCAATCCAAAGTTCATCTGCGATGGCGAACAGCATTGGTTTGCCGAGAATTGGAAGAAGAGTATAGTGCTTTGATTCTGAGCGGGGGTCATAGAGCAGCACTAGTAGATCTTATAAACCAAAAGGAGGTCGTAGGGGATCTTTTCAACCAACTTCGGCTCGCCTCACAAAGGCAAACTAAGGGGCGCCCTGCTCAAACCCTTGCTGCAACAAACTTGGAGGATCAAGAACTCTCAGAAAGCATGCAAAAGCTACTTATCGTTATGCAAAGACTAGACCAGAAAATTGCTCCGATGCTAGAAGCAGATGGAGAGCTCTTCAACAAAAGGTGGGGATTTCTTTCCCGTGCAGGTTTCTGGGATAAAAGCCATTTGATGAGGCAAATTGAGAAGTATGCTGATATATATACCTCCAGGGTGTCCAATTTCCTACATTATACACCGTTCATGTATTTCCGATCACAGGAACAGACACTTGCTCATGATTCGTACTCATACTACTGTTCGAGGTTTAATGGGCCTGCCATGGACGATGAGGTGAACTTTATGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

634

Amino Acids

71.8

Weight (kDa)

5.96

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 144 - 612 5.1e-146 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 507, 879, 1463
Acc36I ACCTGC 1 cut(s) 1688
AccB1I GGYRCC 1 cut(s) 1533
AccBSI CCGCTC 1 cut(s) 1431
AccII CGCG 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 753
AciI CCGC 8 cut(s) 8, 62, 171, 174, 177, 442, 855, 1431
AclWI GGATC 5 cut(s) 977, 1017, 1134, 1494, 1578
AcoI YGGCCR 2 cut(s) 9, 63
AcsI RAATTY 2 cut(s) 553, 1285
AcuI CTGAAG 1 cut(s) 378
AcyI GRCGYC 1 cut(s) 1534
AfaI GTAC 4 cut(s) 5, 227, 731, 1838
AfeI AGCGCT 1 cut(s) 1002
AfiI CCNNNNNNNGG 9 cut(s) 581, 604, 757, 915, 916, 1399, 1474, 1518, 1810
AflIII ACRYGT 1 cut(s) 922
AgsI TTSAA 8 cut(s) 268, 322, 451, 559, 1093, 1234, 1495, 1669
AhdI GACNNNNNGTC 1 cut(s) 193
AhlI ACTAGT 1 cut(s) 1450
AjnI CCWGG 1 cut(s) 1760
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AluBI AGCT 8 cut(s) 293, 365, 533, 716, 806, 1033, 1600, 1663
AluI AGCT 8 cut(s) 293, 365, 533, 716, 806, 1033, 1600, 1663
Alw21I GWGCWC 2 cut(s) 1273, 1665
Alw26I GTCTC 5 cut(s) 38, 363, 375, 802, 1153
AlwI GGATC 5 cut(s) 977, 1017, 1134, 1494, 1578
AlwNI CAGNNNCTG 1 cut(s) 806
Aor13HI TCCGGA 1 cut(s) 753
Aor51HI AGCGCT 1 cut(s) 1002
AoxI GGCC 5 cut(s) 9, 63, 616, 1213, 1865
ApeKI GCWGC 7 cut(s) 123, 165, 168, 180, 1081, 1445, 1553
ApoI RAATTY 2 cut(s) 553, 1285
AspLEI GCGC 2 cut(s) 1003, 1536
AspS9I GGNCC 6 cut(s) 83, 326, 748, 866, 1213, 1865
AsuC2I CCSGG 1 cut(s) 917
AsuHPI GGTGA 5 cut(s) 320, 661, 1220, 1337, 1897
AsuII TTCGAA 1 cut(s) 91
AvaII GGWCC 4 cut(s) 83, 326, 748, 866
BaeGI GKGCMC 1 cut(s) 922
BanI GGYRCC 1 cut(s) 1533
BanII GRGCYC 4 cut(s) 401, 888, 910, 1665
BauI CACGAG 1 cut(s) 423
Bbv12I GWGCWC 2 cut(s) 1273, 1665
BbvI GCAGC 7 cut(s) 135, 177, 180, 192, 1093, 1457, 1540
BccI CCATC 9 cut(s) 81, 299, 762, 817, 836, 1108, 1270, 1365, 1649
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 1 cut(s) 1762
BclI TGATCA 1 cut(s) 1327
BcnI CCSGG 1 cut(s) 917
BcoDI GTCTC 5 cut(s) 38, 363, 375, 802, 1153
BcuI ACTAGT 1 cut(s) 1450
BfaI CTAG 3 cut(s) 1451, 1622, 1646
BfoI RGCGCY 2 cut(s) 1004, 1537
BfuAI ACCTGC 1 cut(s) 1688
BglII AGATCT 1 cut(s) 1456
Bme1390I CCNGG 2 cut(s) 917, 1762
Bme18I GGWCC 4 cut(s) 83, 326, 748, 866
BmeRI GACNNNNNGTC 1 cut(s) 193
BmgT120I GGNCC 6 cut(s) 83, 326, 748, 866, 1213, 1865
BmiI GGNNCC 4 cut(s) 328, 847, 978, 1535
BmrFI CCNGG 2 cut(s) 917, 1762
BmsI GCATC 5 cut(s) 62, 292, 489, 1068, 1632
BoxI GACNNNNGTC 1 cut(s) 103
BplI GAGNNNNNCTC 2 cut(s) 360, 392
BpmI CTGGAG 2 cut(s) 387, 1744
Bpu14I TTCGAA 1 cut(s) 91
BpuMI CCSGG 1 cut(s) 917
Bsa29I ATCGAT 1 cut(s) 661
BsaHI GRCGYC 1 cut(s) 1534
BsaI GGTCTC 1 cut(s) 38
BsaJI CCNNGG 4 cut(s) 12, 1300, 1761, 1872
BsaWI WCCGGW 2 cut(s) 85, 753
Bsc4I CCNNNNNNNGG 9 cut(s) 581, 604, 757, 915, 916, 1399, 1474, 1518, 1810
Bse1I ACTGG 2 cut(s) 160, 747
BseAI TCCGGA 1 cut(s) 753
BseBI CCWGG 1 cut(s) 1762
BseCI ATCGAT 1 cut(s) 661
BseDI CCNNGG 4 cut(s) 12, 1300, 1761, 1872
BseGI GGATG 1 cut(s) 844
BseLI CCNNNNNNNGG 9 cut(s) 581, 604, 757, 915, 916, 1399, 1474, 1518, 1810
BseMII CTCAG 7 cut(s) 91, 391, 813, 1065, 1286, 1418, 1596
BseNI ACTGG 2 cut(s) 160, 747
BseSI GKGCMC 1 cut(s) 922
BseX3I CGGCCG 1 cut(s) 9
BseXI GCAGC 7 cut(s) 135, 177, 180, 192, 1093, 1457, 1540
BsgI GTGCAG 2 cut(s) 184, 1716
Bsh1236I CGCG 1 cut(s) 8
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 5 cut(s) 11, 65, 618, 1215, 1867
BshNI GGYRCC 1 cut(s) 1533
BshVI ATCGAT 1 cut(s) 661
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 1273, 1665
BsiSI CCGG 3 cut(s) 86, 754, 916
BslFI GGGAC 2 cut(s) 91, 312
BslI CCNNNNNNNGG 9 cut(s) 581, 604, 757, 915, 916, 1399, 1474, 1518, 1810
BsmAI GTCTC 5 cut(s) 38, 363, 375, 802, 1153
BsmFI GGGAC 2 cut(s) 91, 312
BsmI GAATGC 2 cut(s) 390, 1084
BsnI GGCC 5 cut(s) 11, 65, 618, 1215, 1867
Bso31I GGTCTC 1 cut(s) 38
Bsp119I TTCGAA 1 cut(s) 91
Bsp1286I GDGCHC 6 cut(s) 401, 888, 910, 922, 1273, 1665
Bsp13I TCCGGA 1 cut(s) 753
Bsp19I CCATGG 2 cut(s) 1300, 1872
BspACI CCGC 8 cut(s) 8, 62, 171, 174, 177, 442, 855, 1431
BspANI GGCC 5 cut(s) 11, 65, 618, 1215, 1867
BspCNI CTCAG 7 cut(s) 90, 390, 812, 1064, 1285, 1419, 1595
BspDI ATCGAT 1 cut(s) 661
BspEI TCCGGA 1 cut(s) 753
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 1828
BspLI GGNNCC 4 cut(s) 328, 847, 978, 1535
BspMI ACCTGC 1 cut(s) 1688
BspPI GGATC 5 cut(s) 977, 1017, 1134, 1494, 1578
BspQI GCTCTTC 2 cut(s) 898, 1670
BspT104I TTCGAA 1 cut(s) 91
BspT107I GGYRCC 1 cut(s) 1533
BspTNI GGTCTC 1 cut(s) 38
BsrBI CCGCTC 1 cut(s) 1431
BsrI ACTGG 2 cut(s) 160, 747
BssECI CCNNGG 4 cut(s) 12, 1300, 1761, 1872
BssNI GRCGYC 1 cut(s) 1534
BssSI CACGAG 1 cut(s) 423
BssT1I CCWWGG 2 cut(s) 1300, 1872
Bst2BI CACGAG 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 1762
Bst4CI ACNGT 7 cut(s) 545, 584, 789, 969, 1317, 1791, 1850
Bst6I CTCTTC 4 cut(s) 898, 917, 1401, 1670
BstACI GRCGYC 1 cut(s) 1534
BstBI TTCGAA 1 cut(s) 91
BstC8I GCNNGC 3 cut(s) 1509, 1592, 1869
BstDEI CTNAG 8 cut(s) 77, 377, 799, 1051, 1272, 1427, 1527, 1582
BstDSI CCRYGG 2 cut(s) 1300, 1872
BstENI CCTNNNNNAGG 2 cut(s) 755, 1516
BstF5I GGATG 1 cut(s) 844
BstFNI CGCG 1 cut(s) 8
BstH2I RGCGCY 2 cut(s) 1004, 1537
BstHHI GCGC 2 cut(s) 1003, 1536
BstMAI GTCTC 5 cut(s) 38, 363, 375, 802, 1153
BstMCI CGRYCG 1 cut(s) 12
BstNI CCWGG 1 cut(s) 1762
BstNSI RCATGY 2 cut(s) 926, 1594
BstPAI GACNNNNGTC 1 cut(s) 103
BstSCI CCNGG 2 cut(s) 915, 1760
BstSLI GKGCMC 1 cut(s) 922
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 7 cut(s) 135, 177, 180, 192, 1093, 1457, 1540
BstX2I RGATCY 3 cut(s) 1126, 1456, 1486
BstXI CCANNNNNNTGG 1 cut(s) 1114
BstYI RGATCY 3 cut(s) 1126, 1456, 1486
BstZI CGGCCG 1 cut(s) 9
Bsu15I ATCGAT 1 cut(s) 661
BsuRI GGCC 5 cut(s) 11, 65, 618, 1215, 1867
BsuTUI ATCGAT 1 cut(s) 661
BtgI CCRYGG 2 cut(s) 1300, 1872
BtgZI GCGATG 1 cut(s) 1384
BtsCI GGATG 1 cut(s) 844
BtsIMutI CAGTG 3 cut(s) 167, 472, 785
BveI ACCTGC 1 cut(s) 1688
Cac8I GCNNGC 3 cut(s) 1509, 1592, 1869
CaiI CAGNNNCTG 1 cut(s) 806
CciI TCATGA 1 cut(s) 1828
CfoI GCGC 2 cut(s) 1003, 1536
Cfr13I GGNCC 6 cut(s) 83, 326, 748, 866, 1213, 1865
ClaI ATCGAT 1 cut(s) 661
CseI GACGC 1 cut(s) 1211
Csp6I GTAC 4 cut(s) 4, 226, 730, 1837
CviQI GTAC 4 cut(s) 4, 226, 730, 1837
DdeI CTNAG 8 cut(s) 77, 377, 799, 1051, 1272, 1427, 1527, 1582
DinI GGCGCC 1 cut(s) 1535
DriI GACNNNNNGTC 1 cut(s) 193
EaeI YGGCCR 2 cut(s) 9, 63
EagI CGGCCG 1 cut(s) 9
Eam1104I CTCTTC 4 cut(s) 898, 917, 1401, 1670
Eam1105I GACNNNNNGTC 1 cut(s) 193
EarI CTCTTC 4 cut(s) 898, 917, 1401, 1670
Ecl136II GAGCTC 1 cut(s) 1663
EclXI CGGCCG 1 cut(s) 9
Eco130I CCWWGG 2 cut(s) 1300, 1872
Eco147I AGGCCT 1 cut(s) 618
Eco24I GRGCYC 4 cut(s) 401, 888, 910, 1665
Eco31I GGTCTC 1 cut(s) 38
Eco47I GGWCC 4 cut(s) 83, 326, 748, 866
Eco47III AGCGCT 1 cut(s) 1002
Eco52I CGGCCG 1 cut(s) 9
Eco53kI GAGCTC 1 cut(s) 1663
Eco57I CTGAAG 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 1663
EcoNI CCTNNNNNAGG 2 cut(s) 755, 1516
EcoO109I RGGNCCY 2 cut(s) 326, 1213
EcoRI GAATTC 1 cut(s) 1285
EcoRII CCWGG 1 cut(s) 1760
EcoT14I CCWWGG 2 cut(s) 1300, 1872
EcoT22I ATGCAT 2 cut(s) 504, 690
EcoT38I GRGCYC 4 cut(s) 401, 888, 910, 1665
EgeI GGCGCC 1 cut(s) 1535
EheI GGCGCC 1 cut(s) 1535
ErhI CCWWGG 2 cut(s) 1300, 1872
FaqI GGGAC 2 cut(s) 91, 312
FauI CCCGC 1 cut(s) 1424
FbaI TGATCA 1 cut(s) 1327
FokI GGATG 1 cut(s) 851
FriOI GRGCYC 4 cut(s) 401, 888, 910, 1665
FspBI CTAG 3 cut(s) 1451, 1622, 1646
GlaI GCGC 2 cut(s) 1002, 1535
GsuI CTGGAG 2 cut(s) 387, 1744
HaeII RGCGCY 2 cut(s) 1004, 1537
HaeIII GGCC 5 cut(s) 11, 65, 618, 1215, 1867
HapII CCGG 3 cut(s) 86, 754, 916
HgaI GACGC 1 cut(s) 1211
HhaI GCGC 2 cut(s) 1003, 1536
Hin1I GRCGYC 1 cut(s) 1534
Hin6I GCGC 2 cut(s) 1001, 1534
HinP1I GCGC 2 cut(s) 1001, 1534
HincII GTYRAC 3 cut(s) 580, 829, 935
HindII GTYRAC 3 cut(s) 580, 829, 935
HindIII AAGCTT 2 cut(s) 291, 1031
HinfI GANTC 5 cut(s) 93, 142, 451, 1423, 1832
HpaII CCGG 3 cut(s) 86, 754, 916
HphI GGTGA 5 cut(s) 320, 661, 1220, 1337, 1897
Hpy166II GTNNAC 7 cut(s) 187, 580, 748, 829, 869, 935, 1888
Hpy188III TCNNGA 6 cut(s) 139, 287, 754, 986, 1574, 1829
Hpy8I GTNNAC 7 cut(s) 187, 580, 748, 829, 869, 935, 1888
Hpy99I CGWCG 1 cut(s) 220
HpyAV CCTTC 3 cut(s) 518, 751, 1099
HpyCH4III ACNGT 7 cut(s) 545, 584, 789, 969, 1317, 1791, 1850
HpyF3I CTNAG 8 cut(s) 77, 377, 799, 1051, 1272, 1427, 1527, 1582
Hsp92I GRCGYC 1 cut(s) 1534
HspAI GCGC 2 cut(s) 1001, 1534
KasI GGCGCC 1 cut(s) 1533
Kpn2I TCCGGA 1 cut(s) 753
Ksp22I TGATCA 1 cut(s) 1327
LguI GCTCTTC 2 cut(s) 898, 1670
LmnI GCTCC 3 cut(s) 280, 976, 1642
Lsp1109I GCAGC 7 cut(s) 135, 177, 180, 192, 1093, 1457, 1540
LweI GCATC 5 cut(s) 62, 292, 489, 1068, 1632
MaeI CTAG 3 cut(s) 1451, 1622, 1646
MaeIII GTNAC 4 cut(s) 208, 313, 1179, 1201
MbiI CCGCTC 1 cut(s) 1431
MflI RGATCY 3 cut(s) 1126, 1456, 1486
MhlI GDGCHC 6 cut(s) 401, 888, 910, 922, 1273, 1665
MluCI AATT 9 cut(s) 300, 553, 861, 989, 1285, 1397, 1632, 1731, 1771
Mly113I GGCGCC 1 cut(s) 1534
MmeI TCCRAC 3 cut(s) 121, 880, 1100
Mph1103I ATGCAT 2 cut(s) 504, 690
MroI TCCGGA 1 cut(s) 753
MseI TTAA 3 cut(s) 675, 780, 1860
MslI CAYNNNNRTG 4 cut(s) 497, 507, 698, 1305
MspA1I CMGCKG 1 cut(s) 171
MspI CCGG 3 cut(s) 86, 754, 916
MspR9I CCNGG 2 cut(s) 917, 1762
Mva1269I GAATGC 2 cut(s) 390, 1084
MvaI CCWGG 1 cut(s) 1762
MvnI CGCG 1 cut(s) 8
NarI GGCGCC 1 cut(s) 1534
NciI CCSGG 1 cut(s) 917
NcoI CCATGG 2 cut(s) 1300, 1872
NlaIV GGNNCC 4 cut(s) 328, 847, 978, 1535
NmeAIII GCCGAG 2 cut(s) 37, 1418
NmuCI GTSAC 3 cut(s) 208, 1179, 1201
NsiI ATGCAT 2 cut(s) 504, 690
NspI RCATGY 2 cut(s) 926, 1594
NspV TTCGAA 1 cut(s) 91
PaeI GCATGC 1 cut(s) 1594
PagI TCATGA 1 cut(s) 1828
PceI AGGCCT 1 cut(s) 618
PciI ACATGT 1 cut(s) 922
PciSI GCTCTTC 2 cut(s) 898, 1670
PctI GAATGC 2 cut(s) 390, 1084
PfeI GAWTC 5 cut(s) 93, 142, 451, 1423, 1832
PflFI GACNNNGTC 1 cut(s) 214
PluTI GGCGCC 1 cut(s) 1537
PpuMI RGGWCCY 1 cut(s) 326
PscI ACATGT 1 cut(s) 922
PshAI GACNNNNGTC 1 cut(s) 103
PsiI TTATAA 3 cut(s) 507, 879, 1463
Psp124BI GAGCTC 1 cut(s) 1665
Psp5II RGGWCCY 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 1760
PspGI CCWGG 1 cut(s) 1760
PspN4I GGNNCC 4 cut(s) 328, 847, 978, 1535
PspPI GGNCC 6 cut(s) 83, 326, 748, 866, 1213, 1865
PspPPI RGGWCCY 1 cut(s) 326
PstNI CAGNNNCTG 1 cut(s) 806
PsuI RGATCY 3 cut(s) 1126, 1456, 1486
PsyI GACNNNGTC 1 cut(s) 214
RsaI GTAC 4 cut(s) 5, 227, 731, 1838
RsaNI GTAC 4 cut(s) 4, 226, 730, 1837
RseI CAYNNNNRTG 4 cut(s) 497, 507, 698, 1305
SacI GAGCTC 1 cut(s) 1665
SapI GCTCTTC 2 cut(s) 898, 1670
SaqAI TTAA 3 cut(s) 675, 780, 1860
Sau96I GGNCC 6 cut(s) 83, 326, 748, 866, 1213, 1865
ScrFI CCNGG 2 cut(s) 917, 1762
SduI GDGCHC 6 cut(s) 401, 888, 910, 922, 1273, 1665
SfaNI GCATC 5 cut(s) 62, 292, 489, 1068, 1632
SfoI GGCGCC 1 cut(s) 1535
SfuI TTCGAA 1 cut(s) 91
SinI GGWCC 4 cut(s) 83, 326, 748, 866
SmiMI CAYNNNNRTG 4 cut(s) 497, 507, 698, 1305
SpeI ACTAGT 1 cut(s) 1450
SphI GCATGC 1 cut(s) 1594
Sse9I AATT 9 cut(s) 300, 553, 861, 989, 1285, 1397, 1632, 1731, 1771
SseBI AGGCCT 1 cut(s) 618
SsiI CCGC 8 cut(s) 8, 62, 171, 174, 177, 442, 855, 1431
SspDI GGCGCC 1 cut(s) 1533
SspMI CTAG 3 cut(s) 1451, 1622, 1646
SstI GAGCTC 1 cut(s) 1665
StuI AGGCCT 1 cut(s) 618
StyD4I CCNGG 2 cut(s) 915, 1760
StyI CCWWGG 2 cut(s) 1300, 1872
TaaI ACNGT 7 cut(s) 545, 584, 789, 969, 1317, 1791, 1850
TasI AATT 9 cut(s) 300, 553, 861, 989, 1285, 1397, 1632, 1731, 1771
TauI GCSGC 7 cut(s) 11, 65, 174, 177, 180, 444, 857
TfiI GAWTC 5 cut(s) 93, 142, 451, 1423, 1832
Tru1I TTAA 3 cut(s) 675, 780, 1860
Tru9I TTAA 3 cut(s) 675, 780, 1860
TscAI CASTG 3 cut(s) 167, 472, 792
TseFI GTSAC 3 cut(s) 208, 1179, 1201
TseI GCWGC 7 cut(s) 123, 165, 168, 180, 1081, 1445, 1553
Tsp45I GTSAC 3 cut(s) 208, 1179, 1201
TspDTI ATGAA 6 cut(s) 83, 106, 167, 1323, 1352, 1783
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 3 cut(s) 167, 472, 792
Tth111I GACNNNGTC 1 cut(s) 214
VpaK11BI GGWCC 4 cut(s) 83, 326, 748, 866
XagI CCTNNNNNAGG 2 cut(s) 755, 1516
XapI RAATTY 2 cut(s) 553, 1285
XceI RCATGY 2 cut(s) 926, 1594
XspI CTAG 3 cut(s) 1451, 1622, 1646
Zsp2I ATGCAT 2 cut(s) 504, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.