AT5G49070

3-ketoacyl-coa synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0020842
Physical Location & Seq
Reverse (-)
19888751 .. 19890174
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G49070.1

Sequence Viewer

Length: 1395 bp
ATGAATCAAACGATACACAGAGTTTCACCAATCTCCATGAGCATCTCAGAGCTGACAACACTTCTCAGCTCAGGTGTTTCTGTCTTTGAGATCTTTGCCGGTTTGCTAGTAGTGCATCTGATCTACCAAAGAATTAGAACAAGAGTCAAAGTCTACTTACTTGATTTCACTTGTTACCGGGCACCTGACTCAAACCGCGTCCCAATGTCAACTCTTATCGAAACCATCTACCTCGATGACAAGCTTGATCAAGAGAGTATTGACTTCCAAGCCCGGATTCTTGAGAGGTCCTGGTTAAGCAACCAAACCAGTATTCCTCGTTCCCTGATGGAAATACCCCTCAAGAAATCTCTCTCCTCGGTCAAAATAGAAACCATGACCACTATCTTCACCTCTGTTGAGGATCTCCTGAGGAAAAACAAACTAAGCCCAAGAAGCATAGACATACTGATAACAAACTGTAGTTTGCATTCGCCTTCACCGTCTCTAAGTGCAATGGTGATCAACAAATTCCATATGAGAAGCAATATCAAGAGCTTTAACCTGTCTGGGATGGGTTGTGCGGCTGGGATTCTATCAGTCAATTTGGCTAATGATTTGCTACAGGCTCACCGAGGCTCATTGGCTCTTATTGTTAGCACCGAAGCGCTGAACACACATTGGTACATTGGCAAAGACAGATCTATGCTTCTTACTAATTGTCTCTTCCGAATGGGAGCAGCTGCAGTTTTGATGTCTAGCAATGATCATGACAGAGACAATGCAAAGTATGAGCTCTTGCATGTTGTTAGAAAAAATAAAGCGAAAGATGATCGAGCTTACCGATGTATCTATCAAGACATAGACTCTGATGAAAAGCAAGGGGTGTCGATAACCAAAGATGTAATCAGCGTTGCTGGGGATATGCTGAAGATGAATCTAACTTCACTGGGACCTCTGGTATTACCTTACTTAGAGCAATTCCAATATGTGATACAACACATTCTTTGCAAGAAACTAAAGATCTATGAGTCGAATTCTTCTTATACACCAAACTTCAAGACAGCGTTTGAGCATTTCTGCATTCATACAGGAGGAAGAGCTGTAATTCAAGCAATGGAGATGAACCTGAAACTGACAAAAGTAGACATCGAGCCATCAAAGATGACTCTTCATAGGTTTGGGAATACTTCTTCGTCATCCATTTGGTACGCTCTCTCTTACTTGGAAGCAAAAAGGAGAATGAAAAAAGGCGATAGGGTCTTGCAGATTGCATTTGGAAGTGGGTTCAAGTGTAACAGTGCGGTGTGGAGATGCATCCGAAAAGTGGAGCCCAACACAGAAAACAAATGGCTCGACTTTATCGACTCATATCCTGTAGACGTACCTGACTCTACAAATATCAGACCAGGCTAG

Protein Analysis

464

Amino Acids

52.56

Weight (kDa)

9.16

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 43 - 332 4.1e-125 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 351 - 431 1.2e-13 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 353 - 433 1.6e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49070 AT5G49070
malus_domestica MD16G1166800.v1.1
prunus_persica Prupe.1G133800_v2.0.a1
pyrus_communis pycom16g14080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 181
AccI GTMKAC 3 cut(s) 153, 1125, 1359
AccII CGCG 1 cut(s) 198
AciI CCGC 3 cut(s) 196, 563, 1283
AclWI GGATC 1 cut(s) 411
AcsI RAATTY 2 cut(s) 509, 1015
AcuI CTGAAG 1 cut(s) 929
AfaI GTAC 3 cut(s) 665, 1190, 1365
AfeI AGCGCT 1 cut(s) 648
AfiI CCNNNNNNNGG 1 cut(s) 1306
AgsI TTSAA 3 cut(s) 1039, 1091, 1270
AjnI CCWGG 2 cut(s) 290, 1387
AjuI GAANNNNNNNTTGG 4 cut(s) 261, 293, 297, 329
AluBI AGCT 8 cut(s) 52, 69, 244, 537, 722, 775, 818, 1082
AluI AGCT 8 cut(s) 52, 69, 244, 537, 722, 775, 818, 1082
Alw21I GWGCWC 1 cut(s) 777
Alw26I GTCTC 3 cut(s) 489, 707, 750
AlwI GGATC 1 cut(s) 411
Aor51HI AGCGCT 1 cut(s) 648
ApeKI GCWGC 2 cut(s) 719, 722
ApoI RAATTY 2 cut(s) 509, 1015
AspLEI GCGC 1 cut(s) 649
AspS9I GGNCC 2 cut(s) 288, 932
AsuC2I CCSGG 2 cut(s) 179, 274
AsuHPI GGTGA 5 cut(s) 18, 382, 471, 511, 602
AvaII GGWCC 2 cut(s) 288, 932
AxyI CCTNAGG 1 cut(s) 410
BaeGI GKGCMC 1 cut(s) 184
BaeI ACNNNNGTAYC 2 cut(s) 811, 844
BanI GGYRCC 1 cut(s) 181
BanII GRGCYC 2 cut(s) 777, 1314
Bbv12I GWGCWC 1 cut(s) 777
BbvI GCAGC 2 cut(s) 709, 731
BccI CCATC 4 cut(s) 233, 322, 547, 1144
BciT130I CCWGG 2 cut(s) 292, 1389
BclI TGATCA 3 cut(s) 247, 501, 745
BcnI CCSGG 2 cut(s) 179, 274
BcoDI GTCTC 3 cut(s) 489, 707, 750
BfaI CTAG 3 cut(s) 107, 738, 1393
BfmI CTRYAG 4 cut(s) 460, 602, 723, 1356
BfoI RGCGCY 1 cut(s) 650
BglII AGATCT 3 cut(s) 90, 680, 1002
BisI GCNGC 3 cut(s) 564, 720, 723
BlsI GCNGC 3 cut(s) 565, 721, 724
Bme1390I CCNGG 4 cut(s) 179, 274, 292, 1389
Bme18I GGWCC 2 cut(s) 288, 932
BmgT120I GGNCC 2 cut(s) 288, 932
BmiI GGNNCC 3 cut(s) 183, 933, 1311
BmrFI CCNGG 4 cut(s) 179, 274, 292, 1389
BmrI ACTGGG 1 cut(s) 938
BmsI GCATC 4 cut(s) 51, 124, 1283, 1305
BmuI ACTGGG 1 cut(s) 938
Bpu10I CCTNAGC 1 cut(s) 70
BpuEI CTTGAG 2 cut(s) 302, 326
BpuMI CCSGG 2 cut(s) 179, 274
BsaJI CCNNGG 2 cut(s) 357, 613
Bsc4I CCNNNNNNNGG 1 cut(s) 1306
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 2 cut(s) 309, 933
Bse21I CCTNAGG 1 cut(s) 410
Bse3DI GCAATG 3 cut(s) 501, 748, 1101
BseBI CCWGG 2 cut(s) 292, 1389
BseDI CCNNGG 2 cut(s) 357, 613
BseGI GGATG 3 cut(s) 558, 1178, 1296
BseLI CCNNNNNNNGG 1 cut(s) 1306
BseMI GCAATG 3 cut(s) 501, 748, 1101
BseMII CTCAG 4 cut(s) 60, 79, 84, 401
BseNI ACTGG 2 cut(s) 309, 933
BseRI GAGGAG 1 cut(s) 346
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 2 cut(s) 709, 731
BseYI CCCAGC 2 cut(s) 566, 896
Bsh1236I CGCG 1 cut(s) 198
BshNI GGYRCC 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 777
BsiSI CCGG 3 cut(s) 99, 178, 274
BslFI GGGAC 2 cut(s) 185, 945
BslI CCNNNNNNNGG 1 cut(s) 1306
BsmAI GTCTC 3 cut(s) 489, 707, 750
BsmBI CGTCTC 1 cut(s) 489
BsmFI GGGAC 2 cut(s) 185, 945
BsmI GAATGC 2 cut(s) 469, 1062
Bsp1286I GDGCHC 3 cut(s) 184, 777, 1314
Bsp143I GATC 9 cut(s) 90, 120, 247, 403, 501, 680, 745, 811, 1002
BspACI CCGC 3 cut(s) 196, 563, 1283
BspCNI CTCAG 4 cut(s) 59, 78, 83, 402
BspFNI CGCG 1 cut(s) 198
BspHI TCATGA 1 cut(s) 748
BspLI GGNNCC 3 cut(s) 183, 933, 1311
BspMAI CTGCAG 1 cut(s) 727
BspPI GGATC 1 cut(s) 411
BspQI GCTCTTC 1 cut(s) 1072
BspT107I GGYRCC 1 cut(s) 181
BsrDI GCAATG 3 cut(s) 501, 748, 1101
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 2 cut(s) 309, 933
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 357, 613
BssMI GATC 9 cut(s) 90, 120, 247, 403, 501, 680, 745, 811, 1002
Bst2UI CCWGG 2 cut(s) 292, 1389
Bst4CI ACNGT 3 cut(s) 461, 483, 1280
Bst6I CTCTTC 3 cut(s) 710, 1072, 1155
BstDEI CTNAG 7 cut(s) 46, 65, 70, 410, 425, 488, 952
BstF5I GGATG 3 cut(s) 558, 1178, 1296
BstFNI CGCG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 650
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 9 cut(s) 93, 123, 250, 406, 504, 683, 748, 814, 1005
BstMAI GTCTC 3 cut(s) 489, 707, 750
BstMBI GATC 9 cut(s) 90, 120, 247, 403, 501, 680, 745, 811, 1002
BstMWI GCNNNNNNNGC 2 cut(s) 112, 435
BstNI CCWGG 2 cut(s) 292, 1389
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 4 cut(s) 177, 272, 290, 1387
BstSFI CTRYAG 4 cut(s) 460, 602, 723, 1356
BstSLI GKGCMC 1 cut(s) 184
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 2 cut(s) 709, 731
BstX2I RGATCY 4 cut(s) 90, 403, 680, 1002
BstYI RGATCY 4 cut(s) 90, 403, 680, 1002
Bsu36I CCTNAGG 1 cut(s) 410
BtsCI GGATG 3 cut(s) 558, 1178, 1296
BtsIMutI CAGTG 2 cut(s) 926, 1285
CciI TCATGA 1 cut(s) 748
CfoI GCGC 1 cut(s) 649
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 2 cut(s) 288, 932
CseI GACGC 1 cut(s) 187
Csp6I GTAC 3 cut(s) 664, 1189, 1364
CviAII CATG 4 cut(s) 37, 376, 749, 782
CviQI GTAC 3 cut(s) 664, 1189, 1364
DdeI CTNAG 7 cut(s) 46, 65, 70, 410, 425, 488, 952
DpnI GATC 9 cut(s) 92, 122, 249, 405, 503, 682, 747, 813, 1004
DpnII GATC 9 cut(s) 90, 120, 247, 403, 501, 680, 745, 811, 1002
Eam1104I CTCTTC 3 cut(s) 710, 1072, 1155
EarI CTCTTC 3 cut(s) 710, 1072, 1155
Ecl136II GAGCTC 1 cut(s) 775
Eco24I GRGCYC 2 cut(s) 777, 1314
Eco47I GGWCC 2 cut(s) 288, 932
Eco47III AGCGCT 1 cut(s) 648
Eco53kI GAGCTC 1 cut(s) 775
Eco57I CTGAAG 1 cut(s) 929
Eco81I CCTNAGG 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 775
EcoO109I RGGNCCY 2 cut(s) 288, 932
EcoRI GAATTC 1 cut(s) 1015
EcoRII CCWGG 2 cut(s) 290, 1387
EcoT22I ATGCAT 1 cut(s) 1298
EcoT38I GRGCYC 2 cut(s) 777, 1314
Esp3I CGTCTC 1 cut(s) 489
FaeI CATG 4 cut(s) 40, 379, 752, 785
FaqI GGGAC 2 cut(s) 185, 945
FatI CATG 4 cut(s) 36, 375, 748, 781
FauNDI CATATG 1 cut(s) 516
FbaI TGATCA 3 cut(s) 247, 501, 745
FblI GTMKAC 3 cut(s) 153, 1125, 1359
Fnu4HI GCNGC 3 cut(s) 564, 720, 723
FokI GGATG 3 cut(s) 565, 1165, 1283
FriOI GRGCYC 2 cut(s) 777, 1314
Fsp4HI GCNGC 3 cut(s) 564, 720, 723
FspBI CTAG 3 cut(s) 107, 738, 1393
GlaI GCGC 1 cut(s) 648
GluI GCNGC 3 cut(s) 564, 720, 723
GsaI CCCAGC 2 cut(s) 570, 900
HaeII RGCGCY 1 cut(s) 650
HapII CCGG 3 cut(s) 99, 178, 274
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 649
Hin1II CATG 4 cut(s) 40, 379, 752, 785
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 242
HpaII CCGG 3 cut(s) 99, 178, 274
HphI GGTGA 5 cut(s) 18, 382, 471, 511, 602
Hpy166II GTNNAC 4 cut(s) 154, 210, 1126, 1360
Hpy188I TCNGA 6 cut(s) 49, 120, 710, 850, 1301, 1385
Hpy188III TCNNGA 8 cut(s) 251, 281, 343, 409, 532, 749, 836, 1039
Hpy8I GTNNAC 4 cut(s) 154, 210, 1126, 1360
HpyAV CCTTC 1 cut(s) 486
HpyCH4III ACNGT 3 cut(s) 461, 483, 1280
HpyCH4IV ACGT 1 cut(s) 1362
HpyF10VI GCNNNNNNNGC 2 cut(s) 112, 435
HpyF3I CTNAG 7 cut(s) 46, 65, 70, 410, 425, 488, 952
HpySE526I ACGT 1 cut(s) 1362
Hsp92II CATG 4 cut(s) 40, 379, 752, 785
HspAI GCGC 1 cut(s) 647
Ksp22I TGATCA 3 cut(s) 247, 501, 745
Kzo9I GATC 9 cut(s) 90, 120, 247, 403, 501, 680, 745, 811, 1002
LguI GCTCTTC 1 cut(s) 1072
LmnI GCTCC 2 cut(s) 716, 1309
Lsp1109I GCAGC 2 cut(s) 709, 731
LweI GCATC 4 cut(s) 51, 124, 1283, 1305
MaeI CTAG 3 cut(s) 107, 738, 1393
MaeII ACGT 1 cut(s) 1362
MaeIII GTNAC 2 cut(s) 173, 1274
MalI GATC 9 cut(s) 92, 122, 249, 405, 503, 682, 747, 813, 1004
MboI GATC 9 cut(s) 90, 120, 247, 403, 501, 680, 745, 811, 1002
MboII GAAGA 7 cut(s) 379, 697, 922, 1011, 1089, 1142, 1164
MflI RGATCY 4 cut(s) 90, 403, 680, 1002
MhlI GDGCHC 3 cut(s) 184, 777, 1314
MluCI AATT 7 cut(s) 132, 509, 583, 697, 959, 1015, 1086
MlyI GAGTC 7 cut(s) 153, 182, 839, 1019, 1141, 1340, 1364
Mph1103I ATGCAT 1 cut(s) 1298
MseI TTAA 2 cut(s) 296, 540
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 3 cut(s) 99, 178, 274
MspR9I CCNGG 4 cut(s) 179, 274, 292, 1389
Mva1269I GAATGC 2 cut(s) 469, 1062
MvaI CCWGG 2 cut(s) 292, 1389
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 2 cut(s) 112, 435
NciI CCSGG 2 cut(s) 179, 274
NdeI CATATG 1 cut(s) 516
NdeII GATC 9 cut(s) 90, 120, 247, 403, 501, 680, 745, 811, 1002
NlaIII CATG 4 cut(s) 40, 379, 752, 785
NlaIV GGNNCC 3 cut(s) 183, 933, 1311
NsiI ATGCAT 1 cut(s) 1298
NspI RCATGY 1 cut(s) 785
PagI TCATGA 1 cut(s) 748
PciSI GCTCTTC 1 cut(s) 1072
PcsI WCGNNNNNNNCGW 3 cut(s) 479, 820, 1341
PctI GAATGC 2 cut(s) 469, 1062
PfeI GAWTC 4 cut(s) 4, 277, 571, 916
PkrI GCNGC 3 cut(s) 565, 721, 724
PleI GAGTC 7 cut(s) 152, 182, 839, 1018, 1141, 1340, 1364
PpsI GAGTC 7 cut(s) 152, 182, 839, 1018, 1141, 1340, 1364
PpuMI RGGWCCY 2 cut(s) 288, 932
Psp124BI GAGCTC 1 cut(s) 777
Psp5II RGGWCCY 2 cut(s) 288, 932
Psp6I CCWGG 2 cut(s) 290, 1387
PspFI CCCAGC 2 cut(s) 566, 896
PspGI CCWGG 2 cut(s) 290, 1387
PspN4I GGNNCC 3 cut(s) 183, 933, 1311
PspPI GGNCC 2 cut(s) 288, 932
PspPPI RGGWCCY 2 cut(s) 288, 932
PsrI GAACNNNNNNTAC 2 cut(s) 304, 336
PstI CTGCAG 1 cut(s) 727
PsuI RGATCY 4 cut(s) 90, 403, 680, 1002
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 3 cut(s) 665, 1190, 1365
RsaNI GTAC 3 cut(s) 664, 1189, 1364
SacI GAGCTC 1 cut(s) 777
SapI GCTCTTC 1 cut(s) 1072
SaqAI TTAA 2 cut(s) 296, 540
SatI GCNGC 3 cut(s) 564, 720, 723
Sau3AI GATC 9 cut(s) 90, 120, 247, 403, 501, 680, 745, 811, 1002
Sau96I GGNCC 2 cut(s) 288, 932
SchI GAGTC 7 cut(s) 153, 182, 839, 1019, 1141, 1340, 1364
ScrFI CCNGG 4 cut(s) 179, 274, 292, 1389
SduI GDGCHC 3 cut(s) 184, 777, 1314
SfaNI GCATC 4 cut(s) 51, 124, 1283, 1305
SfcI CTRYAG 4 cut(s) 460, 602, 723, 1356
SinI GGWCC 2 cut(s) 288, 932
SmlI CTYRAG 2 cut(s) 281, 341
SmoI CTYRAG 2 cut(s) 281, 341
Sse9I AATT 7 cut(s) 132, 509, 583, 697, 959, 1015, 1086
SsiI CCGC 3 cut(s) 196, 563, 1283
SspMI CTAG 3 cut(s) 107, 738, 1393
SstI GAGCTC 1 cut(s) 777
StyD4I CCNGG 4 cut(s) 177, 272, 290, 1387
TaaI ACNGT 3 cut(s) 461, 483, 1280
TaiI ACGT 1 cut(s) 1365
TaqI TCGA 8 cut(s) 219, 234, 814, 869, 1013, 1131, 1335, 1344
TaqII GACCGA 1 cut(s) 349
TasI AATT 7 cut(s) 132, 509, 583, 697, 959, 1015, 1086
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 4 cut(s) 4, 277, 571, 916
Tru1I TTAA 2 cut(s) 296, 540
Tru9I TTAA 2 cut(s) 296, 540
TscAI CASTG 2 cut(s) 933, 1285
TseI GCWGC 2 cut(s) 719, 722
TspDTI ATGAA 7 cut(s) 17, 867, 929, 1055, 1118, 1142, 1238
TspRI CASTG 2 cut(s) 933, 1285
VpaK11BI GGWCC 2 cut(s) 288, 932
XapI RAATTY 2 cut(s) 509, 1015
XceI RCATGY 1 cut(s) 785
XmiI GTMKAC 3 cut(s) 153, 1125, 1359
XspI CTAG 3 cut(s) 107, 738, 1393
Zsp2I ATGCAT 1 cut(s) 1298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.