Prupe.1G133800_v2.0.a1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0020842
Physical Location & Seq
Forward (+)
10576450 .. 10578107
1658 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G133800.1

Sequence Viewer

Length: 1347 bp
ATGGCTAGGATCCAAGAATACATTCTCATTTACCCTGAGCTCATCATCTCCACCGCTATATGTTCTACTATGGCGGCCGCAATTGCAGGCCTTATTCTCATGTATCTTATCCACAAGAGAAAACCCAAAGTTTATTTATTGGACTTCAGCTGCTACCAGCCACCAAACTCTTGTAGGCTGCCCATGTCAATGTTCCTGGAGAATGTCCTTCTGGATGGCACAGACCCTGACAGCATAGCTTTCCAAGTCAAGATCCTAGAGAAATCTGGGTTCAGTGAGGAGACTTGTATTCCTCCATCTCTCACTTGCTTACCTATAAGAAAGTCACTTTCATTTGCTCTGGAAGAGGTCACAACAGTAATGTTTTCTGCAGTTTCGAATTTGTTTGAGAAACACAACATCAACCCGAGAGCGGTCGATATACTGATTTCTAACAGCAGCTTGTTTTGTCCCACACCATCTCTCAGCACTCTGATAATTAACAAGTTTAGAATGAGGAGCAACATAAAGAATTTCAACTTATCTGGTATGGGATGCAGTGCTGGACTTATAGCAGTTAGTCTAGCTAAGGACTTACTGAGAGTTCACAGCAACTCATTGGCTTTAATTGTCAGCACAGAGACCTTAGTTTTAAATTGGTACAAAGGAAAAGTACCATCCATGTTGCTGACCAATTGTTTGTTCCGAATGGGAGGAGCGGCCATGTTAATGTCAAGTCGAAAAAAAGATAAGGGCAGGGCAAAGTATGAGCTGCAACACCTTGTTAGAACAAACAAAGCACAAGATGATCAGTCTCATGCCTGTGTGTTTCAAGATATGGACCCTGAGCACAAACAGGGGATTTCAATATCCAAAGACATACTACATGTTGCTGGAGATGCATTGAAGGCAAACATTGGTGCTCTGGGGCCGATGGTTTTGCCAATTTCAGAGCAGATTCGATATGGGATTTCGATACTCATCCGAAAGATATGGAGCCAAAGGAGGAGGAAGACTCGCATACCGAATTTCAAGAAAGCTTTTGAGCATTTCTGCATACATGCTGGAGGGAGGGCAGTAATACAAGGCATTGAAAGAAACCTGGAGTTAAGAAAAGAAGATGTTGAGGCCTCAAAGATGACTCTGTACAGATTCGGAAATACGTCTTCTTCTTCGTTATGGTACGAGCTGAGTTATATAGAAGCAAAAGGAAAAATGAAGAAGGGAGATAGGGTCTGGCAAATTGCATTTGGGAGCGGCTTCAAGTGTAACAGTGCGGTTTGGAAGTGTGTTGCCAAAGTGGGTGCTGAGAGAGAGAATGTGTGGAGAGACATAATCCATTCATATCCTGTAGAGATTCCAAACTAG

Protein Analysis

449

Amino Acids

50.29

Weight (kDa)

9.41

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49070 AT5G49070
malus_domestica MD16G1166800.v1.1
prunus_persica Prupe.1G133800_v2.0.a1
pyrus_communis pycom16g14080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 413, 698, 1236
AciI CCGC 7 cut(s) 54, 74, 78, 413, 698, 1236, 1256
AclWI GGATC 3 cut(s) 4, 17, 247
AcoI YGGCCR 2 cut(s) 75, 699
AcsI RAATTY 3 cut(s) 379, 511, 1006
AcuI CTGAAG 1 cut(s) 130
AfaI GTAC 4 cut(s) 641, 654, 1127, 1163
AfiI CCNNNNNNNGG 1 cut(s) 412
AflIII ACRYGT 1 cut(s) 865
AgsI TTSAA 7 cut(s) 517, 812, 846, 886, 1012, 1073, 1243
AjnI CCWGG 2 cut(s) 195, 1080
AjuI GAANNNNNNNTTGG 3 cut(s) 38, 665, 697
AluBI AGCT 8 cut(s) 40, 150, 239, 441, 566, 751, 1019, 1168
AluI AGCT 8 cut(s) 40, 150, 239, 441, 566, 751, 1019, 1168
Alw21I GWGCWC 3 cut(s) 42, 831, 904
Alw26I GTCTC 4 cut(s) 275, 614, 798, 1302
AlwI GGATC 3 cut(s) 4, 17, 247
AlwNI CAGNNNCTG 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 406
AoxI GGCC 5 cut(s) 75, 88, 699, 908, 1107
ApeKI GCWGC 4 cut(s) 150, 178, 438, 751
ApoI RAATTY 3 cut(s) 379, 511, 1006
ArsI GACNNNNNNTTYG 2 cut(s) 661, 693
Asp700I GAANNNNTTC 1 cut(s) 21
AspS9I GGNCC 2 cut(s) 820, 908
AsuII TTCGAA 1 cut(s) 377
AvaI CYCGRG 1 cut(s) 406
AvaII GGWCC 1 cut(s) 820
BamHI GGATCC 1 cut(s) 9
BanII GRGCYC 1 cut(s) 42
BbsI GAAGAC 2 cut(s) 998, 1137
Bbv12I GWGCWC 3 cut(s) 42, 831, 904
BbvI GCAGC 4 cut(s) 137, 165, 450, 738
BccI CCATC 5 cut(s) 209, 304, 466, 664, 907
BcgI CGANNNNNNTGC 2 cut(s) 901, 935
BciT130I CCWGG 2 cut(s) 197, 1082
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 4 cut(s) 275, 614, 798, 1302
BfaI CTAG 4 cut(s) 6, 257, 563, 1345
BfmI CTRYAG 2 cut(s) 369, 1329
BisI GCNGC 8 cut(s) 75, 78, 151, 179, 439, 699, 752, 1237
BlsI GCNGC 8 cut(s) 76, 79, 152, 180, 440, 700, 753, 1238
Bme1390I CCNGG 2 cut(s) 197, 1082
Bme18I GGWCC 1 cut(s) 820
BmeT110I CYCGRG 1 cut(s) 406
BmgT120I GGNCC 2 cut(s) 820, 908
BmiI GGNNCC 4 cut(s) 11, 822, 909, 977
BmrFI CCNGG 2 cut(s) 197, 1082
BmsI GCATC 2 cut(s) 524, 868
BpiI GAAGAC 2 cut(s) 998, 1137
BplI GAGNNNNNCTC 2 cut(s) 979, 1011
BpmI CTGGAG 4 cut(s) 218, 894, 1065, 1103
Bpu10I CCTNAGC 3 cut(s) 36, 567, 825
Bpu14I TTCGAA 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 959
BsaI GGTCTC 1 cut(s) 614
BsaXI ACNNNNNCTCC 2 cut(s) 1197, 1227
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse8I GATNNNNATC 1 cut(s) 959
BseBI CCWGG 2 cut(s) 197, 1082
BseGI GGATG 4 cut(s) 220, 539, 656, 960
BseJI GATNNNNATC 1 cut(s) 959
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMII CTCAG 6 cut(s) 27, 478, 569, 816, 1160, 1278
BseRI GAGGAG 4 cut(s) 293, 511, 708, 1000
BseX3I CGGCCG 1 cut(s) 75
BseXI GCAGC 4 cut(s) 137, 165, 450, 738
Bsh1285I CGRYCG 2 cut(s) 78, 417
BshFI GGCC 5 cut(s) 77, 90, 701, 910, 1109
BsiEI CGRYCG 2 cut(s) 78, 417
BsiHKAI GWGCWC 3 cut(s) 42, 831, 904
BsiHKCI CYCGRG 1 cut(s) 406
BslFI GGGAC 1 cut(s) 435
BslI CCNNNNNNNGG 1 cut(s) 412
BsmAI GTCTC 4 cut(s) 275, 614, 798, 1302
BsmFI GGGAC 1 cut(s) 435
BsnI GGCC 5 cut(s) 77, 90, 701, 910, 1109
Bso31I GGTCTC 1 cut(s) 614
BsoBI CYCGRG 1 cut(s) 406
Bsp119I TTCGAA 1 cut(s) 377
Bsp1286I GDGCHC 3 cut(s) 42, 831, 904
Bsp1407I TGTACA 1 cut(s) 1125
Bsp143I GATC 3 cut(s) 9, 252, 787
BspACI CCGC 7 cut(s) 54, 74, 78, 413, 698, 1236, 1256
BspANI GGCC 5 cut(s) 77, 90, 701, 910, 1109
BspCNI CTCAG 6 cut(s) 28, 477, 570, 817, 1161, 1279
BspLI GGNNCC 4 cut(s) 11, 822, 909, 977
BspMAI CTGCAG 1 cut(s) 373
BspPI GGATC 3 cut(s) 4, 17, 247
BspT104I TTCGAA 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 614
BsrBI CCGCTC 3 cut(s) 413, 698, 1236
BsrGI TGTACA 1 cut(s) 1125
BssMI GATC 3 cut(s) 9, 252, 787
Bst2UI CCWGG 2 cut(s) 197, 1082
Bst4CI ACNGT 2 cut(s) 358, 1253
Bst6I CTCTTC 1 cut(s) 339
BstAUI TGTACA 1 cut(s) 1125
BstBI TTCGAA 1 cut(s) 377
BstC8I GCNNGC 1 cut(s) 88
BstDEI CTNAG 8 cut(s) 36, 464, 567, 578, 625, 825, 1169, 1287
BstF5I GGATG 4 cut(s) 220, 539, 656, 960
BstKTI GATC 3 cut(s) 12, 255, 790
BstMAI GTCTC 4 cut(s) 275, 614, 798, 1302
BstMBI GATC 3 cut(s) 9, 252, 787
BstMCI CGRYCG 2 cut(s) 78, 417
BstMWI GCNNNNNNNGC 3 cut(s) 83, 878, 887
BstNI CCWGG 2 cut(s) 197, 1082
BstNSI RCATGY 2 cut(s) 869, 1043
BstSCI CCNGG 2 cut(s) 195, 1080
BstSFI CTRYAG 2 cut(s) 369, 1329
BstV1I GCAGC 4 cut(s) 137, 165, 450, 738
BstV2I GAAGAC 2 cut(s) 998, 1137
BstX2I RGATCY 2 cut(s) 9, 252
BstYI RGATCY 2 cut(s) 9, 252
BstZI CGGCCG 1 cut(s) 75
BsuRI GGCC 5 cut(s) 77, 90, 701, 910, 1109
BtsCI GGATG 4 cut(s) 220, 539, 656, 960
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 3 cut(s) 280, 544, 1258
Cac8I GCNNGC 1 cut(s) 88
CaiI CAGNNNCTG 1 cut(s) 227
CciNI GCGGCCGC 1 cut(s) 75
Cfr13I GGNCC 2 cut(s) 820, 908
Csp6I GTAC 4 cut(s) 640, 653, 1126, 1162
CviAII CATG 7 cut(s) 100, 184, 661, 703, 797, 866, 1040
CviQI GTAC 4 cut(s) 640, 653, 1126, 1162
DdeI CTNAG 8 cut(s) 36, 464, 567, 578, 625, 825, 1169, 1287
DpnI GATC 3 cut(s) 11, 254, 789
DpnII GATC 3 cut(s) 9, 252, 787
DraI TTTAAA 1 cut(s) 633
EaeI YGGCCR 2 cut(s) 75, 699
EagI CGGCCG 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 40
EclXI CGGCCG 1 cut(s) 75
Eco147I AGGCCT 2 cut(s) 90, 1109
Eco24I GRGCYC 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 614
Eco47I GGWCC 1 cut(s) 820
Eco52I CGGCCG 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 40
EcoRII CCWGG 2 cut(s) 195, 1080
EcoT22I ATGCAT 1 cut(s) 883
EcoT38I GRGCYC 1 cut(s) 42
FaeI CATG 7 cut(s) 103, 187, 664, 706, 800, 869, 1043
FaqI GGGAC 1 cut(s) 435
FatI CATG 7 cut(s) 99, 183, 660, 702, 796, 865, 1039
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 8 cut(s) 75, 78, 151, 179, 439, 699, 752, 1237
FokI GGATG 4 cut(s) 227, 546, 643, 947
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 8 cut(s) 75, 78, 151, 179, 439, 699, 752, 1237
FspBI CTAG 4 cut(s) 6, 257, 563, 1345
GluI GCNGC 8 cut(s) 75, 78, 151, 179, 439, 699, 752, 1237
GsuI CTGGAG 4 cut(s) 218, 894, 1065, 1103
HaeIII GGCC 5 cut(s) 77, 90, 701, 910, 1109
Hin1II CATG 7 cut(s) 103, 187, 664, 706, 800, 869, 1043
HindIII AAGCTT 1 cut(s) 1017
HinfI GANTC 5 cut(s) 937, 994, 1120, 1131, 1336
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 5 cut(s) 474, 686, 931, 965, 1136
Hpy188III TCNNGA 5 cut(s) 212, 250, 341, 812, 1012
Hpy8I GTNNAC 1 cut(s) 586
HpyAV CCTTC 3 cut(s) 218, 880, 1195
HpyCH4III ACNGT 2 cut(s) 358, 1253
HpyCH4IV ACGT 1 cut(s) 1142
HpyCH4V TGCA 7 cut(s) 86, 371, 537, 754, 881, 1035, 1226
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 878, 887
HpyF3I CTNAG 8 cut(s) 36, 464, 567, 578, 625, 825, 1169, 1287
HpySE526I ACGT 1 cut(s) 1142
Hsp92II CATG 7 cut(s) 103, 187, 664, 706, 800, 869, 1043
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 3 cut(s) 9, 252, 787
LmnI GCTCC 4 cut(s) 498, 695, 975, 1233
Lsp1109I GCAGC 4 cut(s) 137, 165, 450, 738
LweI GCATC 2 cut(s) 524, 868
MaeI CTAG 4 cut(s) 6, 257, 563, 1345
MaeII ACGT 1 cut(s) 1142
MaeIII GTNAC 3 cut(s) 324, 349, 1247
MalI GATC 3 cut(s) 11, 254, 789
MbiI CCGCTC 3 cut(s) 413, 698, 1236
MboI GATC 3 cut(s) 9, 252, 787
MboII GAAGA 7 cut(s) 356, 1003, 1109, 1137, 1140, 1143, 1210
MfeI CAATTG 2 cut(s) 81, 673
MflI RGATCY 2 cut(s) 9, 252
MhlI GDGCHC 3 cut(s) 42, 831, 904
MlyI GAGTC 2 cut(s) 988, 1114
Mph1103I ATGCAT 1 cut(s) 883
MroXI GAANNNNTTC 1 cut(s) 21
MseI TTAA 5 cut(s) 480, 605, 632, 707, 1088
MslI CAYNNNNRTG 3 cut(s) 188, 707, 801
MspA1I CMGCKG 1 cut(s) 150
MspR9I CCNGG 2 cut(s) 197, 1082
MunI CAATTG 2 cut(s) 81, 673
MvaI CCWGG 2 cut(s) 197, 1082
MwoI GCNNNNNNNGC 3 cut(s) 83, 878, 887
NdeII GATC 3 cut(s) 9, 252, 787
NlaIII CATG 7 cut(s) 103, 187, 664, 706, 800, 869, 1043
NlaIV GGNNCC 4 cut(s) 11, 822, 909, 977
NmuCI GTSAC 2 cut(s) 324, 349
NotI GCGGCCGC 1 cut(s) 75
NsiI ATGCAT 1 cut(s) 883
NspI RCATGY 2 cut(s) 869, 1043
NspV TTCGAA 1 cut(s) 377
PceI AGGCCT 2 cut(s) 90, 1109
PciI ACATGT 1 cut(s) 865
PdmI GAANNNNTTC 1 cut(s) 21
PfeI GAWTC 3 cut(s) 937, 1131, 1336
PfoI TCCNGGA 1 cut(s) 195
PkrI GCNGC 8 cut(s) 76, 79, 152, 180, 440, 700, 753, 1238
PleI GAGTC 2 cut(s) 988, 1114
PpsI GAGTC 2 cut(s) 988, 1114
PscI ACATGT 1 cut(s) 865
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 2 cut(s) 195, 1080
PspGI CCWGG 2 cut(s) 195, 1080
PspN4I GGNNCC 4 cut(s) 11, 822, 909, 977
PspPI GGNCC 2 cut(s) 820, 908
PstI CTGCAG 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 2 cut(s) 9, 252
PvuII CAGCTG 1 cut(s) 150
RsaI GTAC 4 cut(s) 641, 654, 1127, 1163
RsaNI GTAC 4 cut(s) 640, 653, 1126, 1162
RseI CAYNNNNRTG 3 cut(s) 188, 707, 801
SacI GAGCTC 1 cut(s) 42
SaqAI TTAA 5 cut(s) 480, 605, 632, 707, 1088
SatI GCNGC 8 cut(s) 75, 78, 151, 179, 439, 699, 752, 1237
Sau3AI GATC 3 cut(s) 9, 252, 787
Sau96I GGNCC 2 cut(s) 820, 908
SchI GAGTC 2 cut(s) 988, 1114
ScrFI CCNGG 2 cut(s) 197, 1082
SduI GDGCHC 3 cut(s) 42, 831, 904
SfaNI GCATC 2 cut(s) 524, 868
SfcI CTRYAG 2 cut(s) 369, 1329
SfuI TTCGAA 1 cut(s) 377
SinI GGWCC 1 cut(s) 820
SmiMI CAYNNNNRTG 3 cut(s) 188, 707, 801
SseBI AGGCCT 2 cut(s) 90, 1109
SsiI CCGC 7 cut(s) 54, 74, 78, 413, 698, 1236, 1256
SspMI CTAG 4 cut(s) 6, 257, 563, 1345
SstI GAGCTC 1 cut(s) 42
StuI AGGCCT 2 cut(s) 90, 1109
StyD4I CCNGG 2 cut(s) 195, 1080
TaaI ACNGT 2 cut(s) 358, 1253
TaiI ACGT 1 cut(s) 1145
TaqI TCGA 5 cut(s) 377, 417, 718, 940, 953
TatI WGTACW 1 cut(s) 1125
TauI GCSGC 4 cut(s) 77, 80, 701, 1239
TfiI GAWTC 3 cut(s) 937, 1131, 1336
Tru1I TTAA 5 cut(s) 480, 605, 632, 707, 1088
Tru9I TTAA 5 cut(s) 480, 605, 632, 707, 1088
TscAI CASTG 3 cut(s) 280, 544, 1258
TseFI GTSAC 2 cut(s) 324, 349
TseI GCWGC 4 cut(s) 150, 178, 438, 751
Tsp45I GTSAC 2 cut(s) 324, 349
TspDTI ATGAA 3 cut(s) 321, 1211, 1311
TspRI CASTG 3 cut(s) 280, 544, 1258
VpaK11BI GGWCC 1 cut(s) 820
XapI RAATTY 3 cut(s) 379, 511, 1006
XceI RCATGY 2 cut(s) 869, 1043
XmnI GAANNNNTTC 1 cut(s) 21
XspI CTAG 4 cut(s) 6, 257, 563, 1345
Zsp2I ATGCAT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.