pycom16g14080

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0020842
Physical Location & Seq
Forward (+)
10115536 .. 10116818
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g14080.1

Sequence Viewer

Length: 1242 bp
ATGGCTAGGATCCAGGAATACGTTCATGTTTTCCCTGACCTCGTCGAACCCGCCACTGCGTGTTATACGATGGCGGCGATTGCAGGCCTTGTTCTCATGTATCTTCTCCGCAAGGGAGAGCCAAGAATTTATTTGGTAGACTTCAGCTGTTACCAGCCACCAAAATCTTGTAGGCTGCCGACGTCCATGTTCCTAGAGAAGGTCCTTCTGGACGGCACAGACCCTGAGAGCGCAGCATTCCAAATCAAGATCCTTGAGAAATCTGGGTTCAGCGAGGAGACTAGCATTCCTCCGTCTCTCGCTTGCTTGCCTATCAAGAAATCACTCCAATTTGCGTTGGAAGAGGTCAAAGCAGTGATGTTTGGTGCAGTTTCAGACTTGTTCAACAAGCACAACATAAACCCTAGAGAAATTGATGTTCTGATTTCTAACAGCAGCTTGTTTTGTCCCACACCGTCTCTCAGCACTTTGATCATCAACAAGTTTGGAATGAGAAGCAACATCAGAAGCTTTCACTTATCCGGGATGGGATGCAGCGCCGGACTTATTGCAGTTGGACTAGCTAAGGACTTGCTGAGGGTTCACGAGAACTCATTGGCTTTGATTGTCAGCACTGAGGGATTGCATTTGAATTGGTACACCGGAAAAGTACGGTCCATGTTGCTGACTAATTGTTTGTTTCGGATAGGAGGAGCGGCCGTGTTGATGTCCAGTCGGAATCAAGACAAGGGCAGGGCAAAGTATGAGTTGCAGCACCTTGTGAGAACAAACAATGCACAAGATGATCGGTCTCATGCCTGTGTGTTTCAGGATATAGATCCCGAGAACAAACAGGGGATTTCGATATCGAAGGACATACTCCATGTTGCTGGAGACGCATTGAAGGCAAACATTGGTGCTCTGGGACCATTGGTTTTGCCACTTTCGGAGCAGTTTCGATATGGGGTTTCGATACTCAGCCGAAAGGTATGGAGTGGGAGGAGGAAGATTCACGTACCGGATTTTAAGAAAGCTTTCGAGCATTTCTGCATACATGCTGGGGGGAGAGCAGTTATACAAGGAATAGAGAAAAACCTGGAGCTGAGAAAAGAAGACGTGGAGGCCTCAAAGATGGCCCTGTACAGATATGGAAACACATCATCTTCTTCGATATGGTACGAGCTGAGTTACATAGAAGCGAAAGGAAAGATGAAGAAGGGCAATCGGGTGTGGCAAATTGGGTTCGGGAGCGGCTTCAACTGA

Protein Analysis

414

Amino Acids

45.88

Weight (kDa)

9.13

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 40 - 323 1.8e-116 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 340 - 412 1.1e-09 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 342 - 406 7.5e-11 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49070 AT5G49070
malus_domestica MD16G1166800.v1.1
prunus_persica Prupe.1G133800_v2.0.a1
pyrus_communis pycom16g14080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 185
AccBSI CCGCTC 2 cut(s) 695, 1230
AccI GTMKAC 1 cut(s) 138
AciI CCGC 5 cut(s) 51, 74, 109, 695, 1230
AclWI GGATC 4 cut(s) 4, 17, 244, 812
AcoI YGGCCR 1 cut(s) 696
AcsI RAATTY 1 cut(s) 126
AcuI CTGAAG 1 cut(s) 127
AcyI GRCGYC 1 cut(s) 182
AdeI CACNNNGTG 2 cut(s) 60, 760
AfaI GTAC 5 cut(s) 638, 651, 996, 1121, 1157
AfiI CCNNNNNNNGG 1 cut(s) 199
AgsI TTSAA 4 cut(s) 385, 631, 883, 1237
AjiI CACGTC 1 cut(s) 1096
AjnI CCWGG 2 cut(s) 12, 1074
AluBI AGCT 7 cut(s) 147, 438, 510, 563, 1013, 1081, 1162
AluI AGCT 7 cut(s) 147, 438, 510, 563, 1013, 1081, 1162
Alw21I GWGCWC 1 cut(s) 901
Alw26I GTCTC 5 cut(s) 272, 300, 462, 795, 867
AlwI GGATC 4 cut(s) 4, 17, 244, 812
AlwNI CAGNNNCTG 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 821
AoxI GGCC 4 cut(s) 85, 696, 1101, 1113
ApeKI GCWGC 5 cut(s) 175, 233, 435, 534, 751
ApoI RAATTY 1 cut(s) 126
ArsI GACNNNNNNTTYG 2 cut(s) 658, 690
Asp700I GAANNNNTTC 2 cut(s) 21, 1013
AspLEI GCGC 2 cut(s) 233, 539
AspS9I GGNCC 4 cut(s) 202, 654, 905, 1114
AsuC2I CCSGG 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 821
AvaII GGWCC 3 cut(s) 202, 654, 905
BamHI GGATCC 1 cut(s) 9
BauI CACGAG 1 cut(s) 584
BbsI GAAGAC 1 cut(s) 1098
Bbv12I GWGCWC 1 cut(s) 901
BbvCI CCTCAGC 1 cut(s) 575
BbvI GCAGC 5 cut(s) 162, 245, 447, 546, 763
BccI CCATC 3 cut(s) 64, 520, 1105
BceAI ACGGC 2 cut(s) 229, 683
BciT130I CCWGG 2 cut(s) 14, 1076
BclI TGATCA 1 cut(s) 471
BcnI CCSGG 1 cut(s) 523
BcoDI GTCTC 5 cut(s) 272, 300, 462, 795, 867
BfaI CTAG 5 cut(s) 6, 194, 282, 405, 560
BfoI RGCGCY 1 cut(s) 540
BisI GCNGC 8 cut(s) 75, 176, 234, 436, 535, 696, 752, 1231
BlsI GCNGC 8 cut(s) 76, 177, 235, 437, 536, 697, 753, 1232
Bme1390I CCNGG 3 cut(s) 14, 523, 1076
Bme18I GGWCC 3 cut(s) 202, 654, 905
BmeT110I CYCGRG 1 cut(s) 821
BmgBI CACGTC 1 cut(s) 1096
BmgT120I GGNCC 4 cut(s) 202, 654, 905, 1114
BmiI GGNNCC 2 cut(s) 11, 906
BmrFI CCNGG 3 cut(s) 14, 523, 1076
BmsI GCATC 1 cut(s) 521
BpiI GAAGAC 1 cut(s) 1098
BpmI CTGGAG 2 cut(s) 891, 1097
Bpu10I CCTNAGC 2 cut(s) 564, 575
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 523
BsaAI YACGTR 1 cut(s) 994
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 1 cut(s) 182
BsaI GGTCTC 1 cut(s) 795
BsaWI WCCGGW 2 cut(s) 641, 997
BsaXI ACNNNNNCTCC 2 cut(s) 684, 714
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 1 cut(s) 711
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 2 cut(s) 14, 1076
BseGI GGATG 2 cut(s) 531, 536
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMII CTCAG 7 cut(s) 216, 475, 566, 606, 970, 1073, 1154
BseNI ACTGG 1 cut(s) 711
BseRI GAGGAG 3 cut(s) 290, 705, 994
BseX3I CGGCCG 1 cut(s) 696
BseXI GCAGC 5 cut(s) 162, 245, 447, 546, 763
BseYI CCCAGC 1 cut(s) 1037
BsgI GTGCAG 1 cut(s) 387
Bsh1285I CGRYCG 1 cut(s) 699
BshFI GGCC 4 cut(s) 87, 698, 1103, 1115
BsiEI CGRYCG 1 cut(s) 699
BsiHKAI GWGCWC 1 cut(s) 901
BsiHKCI CYCGRG 1 cut(s) 821
BsiSI CCGG 4 cut(s) 522, 540, 642, 998
BslFI GGGAC 2 cut(s) 432, 918
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 5 cut(s) 272, 300, 462, 795, 867
BsmBI CGTCTC 3 cut(s) 300, 462, 867
BsmFI GGGAC 2 cut(s) 432, 918
BsmI GAATGC 2 cut(s) 236, 285
BsnI GGCC 4 cut(s) 87, 698, 1103, 1115
Bso31I GGTCTC 1 cut(s) 795
BsoBI CYCGRG 1 cut(s) 821
Bsp1286I GDGCHC 1 cut(s) 901
Bsp1407I TGTACA 1 cut(s) 1119
Bsp143I GATC 5 cut(s) 9, 249, 471, 784, 817
BspACI CCGC 5 cut(s) 51, 74, 109, 695, 1230
BspANI GGCC 4 cut(s) 87, 698, 1103, 1115
BspCNI CTCAG 7 cut(s) 217, 474, 567, 607, 969, 1074, 1155
BspLI GGNNCC 2 cut(s) 11, 906
BspPI GGATC 4 cut(s) 4, 17, 244, 812
BspTNI GGTCTC 1 cut(s) 795
BsrBI CCGCTC 2 cut(s) 695, 1230
BsrGI TGTACA 1 cut(s) 1119
BsrI ACTGG 1 cut(s) 711
BssMI GATC 5 cut(s) 9, 249, 471, 784, 817
BssNI GRCGYC 1 cut(s) 182
BssSI CACGAG 1 cut(s) 584
Bst2BI CACGAG 1 cut(s) 584
Bst2UI CCWGG 2 cut(s) 14, 1076
Bst4CI ACNGT 2 cut(s) 456, 654
Bst6I CTCTTC 1 cut(s) 336
BstACI GRCGYC 1 cut(s) 182
BstAUI TGTACA 1 cut(s) 1119
BstBAI YACGTR 1 cut(s) 994
BstC8I GCNNGC 3 cut(s) 85, 304, 308
BstDEI CTNAG 8 cut(s) 225, 461, 564, 575, 615, 956, 1082, 1163
BstENI CCTNNNNNAGG 1 cut(s) 197
BstF5I GGATG 2 cut(s) 531, 536
BstH2I RGCGCY 1 cut(s) 540
BstHHI GCGC 2 cut(s) 233, 539
BstKTI GATC 5 cut(s) 12, 252, 474, 787, 820
BstMAI GTCTC 5 cut(s) 272, 300, 462, 795, 867
BstMBI GATC 5 cut(s) 9, 249, 471, 784, 817
BstMCI CGRYCG 1 cut(s) 699
BstMWI GCNNNNNNNGC 3 cut(s) 80, 875, 884
BstNI CCWGG 2 cut(s) 14, 1076
BstNSI RCATGY 1 cut(s) 1037
BstSCI CCNGG 3 cut(s) 12, 521, 1074
BstV1I GCAGC 5 cut(s) 162, 245, 447, 546, 763
BstV2I GAAGAC 1 cut(s) 1098
BstX2I RGATCY 3 cut(s) 9, 249, 817
BstXI CCANNNNNNTGG 1 cut(s) 869
BstYI RGATCY 3 cut(s) 9, 249, 817
BstZI CGGCCG 1 cut(s) 696
BsuRI GGCC 4 cut(s) 87, 698, 1103, 1115
BtrI CACGTC 1 cut(s) 1096
BtsCI GGATG 2 cut(s) 531, 536
BtsI GCAGTG 2 cut(s) 54, 360
BtsIMutI CAGTG 3 cut(s) 54, 360, 612
Cac8I GCNNGC 3 cut(s) 85, 304, 308
CaiI CAGNNNCTG 1 cut(s) 224
CfoI GCGC 2 cut(s) 233, 539
Cfr13I GGNCC 4 cut(s) 202, 654, 905, 1114
CseI GACGC 1 cut(s) 884
Csp6I GTAC 5 cut(s) 637, 650, 995, 1120, 1156
CviAII CATG 7 cut(s) 26, 97, 187, 658, 794, 863, 1034
CviQI GTAC 5 cut(s) 637, 650, 995, 1120, 1156
DdeI CTNAG 8 cut(s) 225, 461, 564, 575, 615, 956, 1082, 1163
DpnI GATC 5 cut(s) 11, 251, 473, 786, 819
DpnII GATC 5 cut(s) 9, 249, 471, 784, 817
DraIII CACNNNGTG 2 cut(s) 60, 760
EaeI YGGCCR 1 cut(s) 696
EagI CGGCCG 1 cut(s) 696
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
EclXI CGGCCG 1 cut(s) 696
Eco147I AGGCCT 2 cut(s) 87, 1103
Eco31I GGTCTC 1 cut(s) 795
Eco32I GATATC 1 cut(s) 846
Eco47I GGWCC 3 cut(s) 202, 654, 905
Eco52I CGGCCG 1 cut(s) 696
Eco57I CTGAAG 1 cut(s) 127
Eco88I CYCGRG 1 cut(s) 821
EcoNI CCTNNNNNAGG 1 cut(s) 197
EcoO109I RGGNCCY 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 12, 1074
EcoRV GATATC 1 cut(s) 846
Esp3I CGTCTC 3 cut(s) 300, 462, 867
FaeI CATG 7 cut(s) 29, 100, 190, 661, 797, 866, 1037
FaqI GGGAC 2 cut(s) 432, 918
FatI CATG 7 cut(s) 25, 96, 186, 657, 793, 862, 1033
FauI CCCGC 1 cut(s) 58
FbaI TGATCA 1 cut(s) 471
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 8 cut(s) 75, 176, 234, 436, 535, 696, 752, 1231
FokI GGATG 2 cut(s) 538, 543
Fsp4HI GCNGC 8 cut(s) 75, 176, 234, 436, 535, 696, 752, 1231
FspBI CTAG 5 cut(s) 6, 194, 282, 405, 560
GlaI GCGC 2 cut(s) 232, 538
GluI GCNGC 8 cut(s) 75, 176, 234, 436, 535, 696, 752, 1231
GsaI CCCAGC 1 cut(s) 1041
GsuI CTGGAG 2 cut(s) 891, 1097
HaeII RGCGCY 1 cut(s) 540
HaeIII GGCC 4 cut(s) 87, 698, 1103, 1115
HapII CCGG 4 cut(s) 522, 540, 642, 998
HgaI GACGC 1 cut(s) 884
HhaI GCGC 2 cut(s) 233, 539
Hin1I GRCGYC 1 cut(s) 182
Hin1II CATG 7 cut(s) 29, 100, 190, 661, 797, 866, 1037
Hin6I GCGC 2 cut(s) 231, 537
HinP1I GCGC 2 cut(s) 231, 537
HindIII AAGCTT 2 cut(s) 508, 1011
HinfI GANTC 2 cut(s) 718, 988
HpaII CCGG 4 cut(s) 522, 540, 642, 998
Hpy166II GTNNAC 3 cut(s) 139, 583, 639
Hpy188I TCNGA 6 cut(s) 376, 423, 506, 684, 717, 928
Hpy188III TCNNGA 8 cut(s) 209, 247, 316, 584, 722, 809, 821, 1225
Hpy8I GTNNAC 3 cut(s) 139, 583, 639
Hpy99I CGWCG 2 cut(s) 47, 184
HpyAV CCTTC 5 cut(s) 193, 215, 844, 877, 1189
HpyCH4III ACNGT 2 cut(s) 456, 654
HpyCH4IV ACGT 4 cut(s) 21, 182, 993, 1095
HpyCH4V TGCA 8 cut(s) 83, 368, 534, 551, 625, 751, 776, 1029
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 875, 884
HpyF3I CTNAG 8 cut(s) 225, 461, 564, 575, 615, 956, 1082, 1163
HpySE526I ACGT 4 cut(s) 21, 182, 993, 1095
Hsp92I GRCGYC 1 cut(s) 182
Hsp92II CATG 7 cut(s) 29, 100, 190, 661, 797, 866, 1037
HspAI GCGC 2 cut(s) 231, 537
Ksp22I TGATCA 1 cut(s) 471
Kzo9I GATC 5 cut(s) 9, 249, 471, 784, 817
LmnI GCTCC 4 cut(s) 692, 928, 1078, 1227
Lsp1109I GCAGC 5 cut(s) 162, 245, 447, 546, 763
LweI GCATC 1 cut(s) 521
MaeI CTAG 5 cut(s) 6, 194, 282, 405, 560
MaeII ACGT 4 cut(s) 21, 182, 993, 1095
MaeIII GTNAC 2 cut(s) 149, 1166
MalI GATC 5 cut(s) 11, 251, 473, 786, 819
MbiI CCGCTC 2 cut(s) 695, 1230
MboI GATC 5 cut(s) 9, 249, 471, 784, 817
MboII GAAGA 7 cut(s) 95, 353, 997, 1103, 1134, 1137, 1204
MflI RGATCY 3 cut(s) 9, 249, 817
MhlI GDGCHC 1 cut(s) 901
MluCI AATT 6 cut(s) 126, 329, 411, 631, 670, 1215
MmeI TCCRAC 3 cut(s) 318, 535, 695
MroXI GAANNNNTTC 2 cut(s) 21, 1013
MseI TTAA 1 cut(s) 1005
MslI CAYNNNNRTG 1 cut(s) 798
MspA1I CMGCKG 1 cut(s) 147
MspI CCGG 4 cut(s) 522, 540, 642, 998
MspR9I CCNGG 3 cut(s) 14, 523, 1076
Mva1269I GAATGC 2 cut(s) 236, 285
MvaI CCWGG 2 cut(s) 14, 1076
MwoI GCNNNNNNNGC 3 cut(s) 80, 875, 884
NciI CCSGG 1 cut(s) 523
NdeII GATC 5 cut(s) 9, 249, 471, 784, 817
NlaIII CATG 7 cut(s) 29, 100, 190, 661, 797, 866, 1037
NlaIV GGNNCC 2 cut(s) 11, 906
NspI RCATGY 1 cut(s) 1037
PceI AGGCCT 2 cut(s) 87, 1103
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PctI GAATGC 2 cut(s) 236, 285
PdmI GAANNNNTTC 2 cut(s) 21, 1013
PfeI GAWTC 2 cut(s) 718, 988
PflFI GACNNNGTC 1 cut(s) 41
PfoI TCCNGGA 2 cut(s) 12, 521
PkrI GCNGC 8 cut(s) 76, 177, 235, 437, 536, 697, 753, 1232
Ppu21I YACGTR 1 cut(s) 994
PpuMI RGGWCCY 1 cut(s) 202
Psp5II RGGWCCY 1 cut(s) 202
Psp6I CCWGG 2 cut(s) 12, 1074
PspFI CCCAGC 1 cut(s) 1037
PspGI CCWGG 2 cut(s) 12, 1074
PspN4I GGNNCC 2 cut(s) 11, 906
PspPI GGNCC 4 cut(s) 202, 654, 905, 1114
PspPPI RGGWCCY 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 3 cut(s) 9, 249, 817
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 147
RsaI GTAC 5 cut(s) 638, 651, 996, 1121, 1157
RsaNI GTAC 5 cut(s) 637, 650, 995, 1120, 1156
RseI CAYNNNNRTG 1 cut(s) 798
SaqAI TTAA 1 cut(s) 1005
SatI GCNGC 8 cut(s) 75, 176, 234, 436, 535, 696, 752, 1231
Sau3AI GATC 5 cut(s) 9, 249, 471, 784, 817
Sau96I GGNCC 4 cut(s) 202, 654, 905, 1114
ScrFI CCNGG 3 cut(s) 14, 523, 1076
SduI GDGCHC 1 cut(s) 901
SfaNI GCATC 1 cut(s) 521
SinI GGWCC 3 cut(s) 202, 654, 905
SmiMI CAYNNNNRTG 1 cut(s) 798
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 6 cut(s) 126, 329, 411, 631, 670, 1215
SseBI AGGCCT 2 cut(s) 87, 1103
SsiI CCGC 5 cut(s) 51, 74, 109, 695, 1230
SspMI CTAG 5 cut(s) 6, 194, 282, 405, 560
StuI AGGCCT 2 cut(s) 87, 1103
StyD4I CCNGG 3 cut(s) 12, 521, 1074
TaaI ACNGT 2 cut(s) 456, 654
TaiI ACGT 4 cut(s) 24, 185, 996, 1098
TaqI TCGA 7 cut(s) 45, 842, 848, 937, 950, 1017, 1148
TaqII GACCGA 1 cut(s) 777
TasI AATT 6 cut(s) 126, 329, 411, 631, 670, 1215
TatI WGTACW 1 cut(s) 1119
TauI GCSGC 3 cut(s) 77, 698, 1233
TfiI GAWTC 2 cut(s) 718, 988
Tru1I TTAA 1 cut(s) 1005
Tru9I TTAA 1 cut(s) 1005
TscAI CASTG 3 cut(s) 61, 360, 619
TseI GCWGC 5 cut(s) 175, 233, 435, 534, 751
TspDTI ATGAA 2 cut(s) 14, 1205
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 3 cut(s) 61, 360, 619
Tth111I GACNNNGTC 1 cut(s) 41
VpaK11BI GGWCC 3 cut(s) 202, 654, 905
XagI CCTNNNNNAGG 1 cut(s) 197
XapI RAATTY 1 cut(s) 126
XceI RCATGY 1 cut(s) 1037
XmiI GTMKAC 1 cut(s) 138
XmnI GAANNNNTTC 2 cut(s) 21, 1013
XspI CTAG 5 cut(s) 6, 194, 282, 405, 560
ZraI GACGTC 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.