MD16G1166800.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0020842
Physical Location & Seq
Forward (+)
13890600 .. 13892014
1415 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1166800.v1.1.491

Sequence Viewer

Length: 1395 bp
ATGTGCCATGAAAATATCTCACTAAACTCTGCCATCATCCATCATCCATCATCCATGGCTAGGATCCAGGAATACGTTCATGTTTTCCCTGACCTCGTCGAACCCGCCACTGCATGTTATATGATGGCGGCGATTGCAGGCCTTGTTCTCATGTATCTTCTCCGCAAGAGAGAGCCAAGAATTTATTTGGTAGACTTTAGCTGTTACCAGCCACCAAAATCTTATAGGCTGACGACGTCCATGTTCCTAGAGAACGTCCTTCTGGACGGCACAGACCCTGAGAGCGCAGCATTCCAAATCAAGATCCTTGAGAAATCCGGGTTCAGCGAGGAGACTAGCATTCCTCCGTCTCTCGCTTGCTTGCCTATCAAGAAATCACTCCAATTTGCGTTGGAAGAGGTCAAAGCAGTGATGTTTGGTGCAGTTTCAGACTTGTTCAACAAGCACAACATAAACCCTAGAGAAATTGATGTTCTGATTTCTAACAGCAGCTTGTTTTGTCCCACACCGTCTCTCAGCACTTTGATCATCAACAAGTTCGGAATGAGAAACAACATCAGAAGCTTCCACTTATCCGGGATGGGATGCAGCGCCGGACTTATTGCAGTTGGTCTAGCCAAGGACTTGCTGAGGGTTCACGAGAACTCATTGGCTTTGATTGTCAGCACTGAGGGATTGCATCTGAATTGGTACACCGGAAAAGTACCGTCCATGTTGCTGACTAATTGTTTGTTTCGGATGGGAGGAGCGGCCGTGTTGATGTCCAGTCGGAATCAAGACAAGAGCAGGGCAAAGTATGAGCTGCAGCATCTTGTCAGAACAAACAATGCACAAGATGATCAGTCTCATGCCTGTGTGTTCCAGGATATAGATCCCGAGAACAAACAGGGGATTTCGATATCAAAGAACATACTCCATGTTGCCGGAGATGCATTGAAAGCAAACATTGGTGCTCTGGGGCCATTGGTTTTGCCACTTTCGGAGCAGTTTCGATATGGGGTTTCGATACTCAGCCGAAAGGTATGGAGTGGGAGGAGGAAGATTCACGTACCGGATTTTAAGAAAGCTTTCGAGCATTTCTGCATACATGCTGGGGGGAGAGCGGTTATACAAGGAATAGAGAAAAACCTGGGGCTGAGAAAAGAAGACGTCGAGGCCTCAAAGATGGCCCTGTACAGATATGGAAACACATCGTCTTCATCGATATGGTACGAGCTGAGTTACATAGAAGCGAAAGGAAGGATGAAGAAGGGCGATCGGGTGTGGCAAATTGGGTTCGGGAGCGGCTTCAAGTGTAACAGCGCGGTGTGGAAGTGTGTTTCCGATGTGGGAGCTGAGAGTGCAAACGTGTGGAGAGAAATAATCCATTCATATCCCATGGAGGTTCCAAACTGA

Protein Analysis

465

Amino Acids

51.66

Weight (kDa)

8.82

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 55 - 341 5.5e-116 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 358 - 438 8.9e-13 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 360 - 436 6.7e-11 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49070 AT5G49070
malus_domestica MD16G1166800.v1.1
prunus_persica Prupe.1G133800_v2.0.a1
pyrus_communis pycom16g14080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 239, 1152
AccBSI CCGCTC 3 cut(s) 749, 1103, 1284
AccI GTMKAC 1 cut(s) 192
AccII CGCG 1 cut(s) 1304
AciI CCGC 7 cut(s) 105, 128, 163, 749, 1103, 1284, 1304
AclWI GGATC 4 cut(s) 58, 71, 298, 866
AcoI YGGCCR 1 cut(s) 750
AcsI RAATTY 1 cut(s) 180
AcyI GRCGYC 2 cut(s) 236, 1149
AfaI GTAC 5 cut(s) 692, 705, 1050, 1175, 1211
AfiI CCNNNNNNNGG 1 cut(s) 60
AflIII ACRYGT 1 cut(s) 1347
AgsI TTSAA 3 cut(s) 439, 937, 1291
AjnI CCWGG 3 cut(s) 66, 861, 1128
AluBI AGCT 7 cut(s) 201, 492, 564, 802, 1067, 1216, 1334
AluI AGCT 7 cut(s) 201, 492, 564, 802, 1067, 1216, 1334
Alw21I GWGCWC 1 cut(s) 955
Alw26I GTCTC 4 cut(s) 326, 354, 516, 849
AlwI GGATC 4 cut(s) 58, 71, 298, 866
AlwNI CAGNNNCTG 1 cut(s) 278
Ama87I CYCGRG 1 cut(s) 875
AoxI GGCC 5 cut(s) 139, 750, 959, 1155, 1167
ApeKI GCWGC 5 cut(s) 287, 489, 588, 802, 805
ApoI RAATTY 1 cut(s) 180
ArsI GACNNNNNNTTYG 2 cut(s) 712, 744
Asp700I GAANNNNTTC 2 cut(s) 75, 1067
AspLEI GCGC 3 cut(s) 287, 593, 1304
AspS9I GGNCC 2 cut(s) 959, 1168
AsuC2I CCSGG 2 cut(s) 319, 577
AvaI CYCGRG 1 cut(s) 875
BamHI GGATCC 1 cut(s) 63
BauI CACGAG 1 cut(s) 638
BbsI GAAGAC 2 cut(s) 1152, 1188
Bbv12I GWGCWC 1 cut(s) 955
BbvCI CCTCAGC 1 cut(s) 629
BbvI GCAGC 5 cut(s) 299, 501, 600, 789, 817
BccI CCATC 7 cut(s) 41, 48, 55, 118, 574, 733, 1159
BceAI ACGGC 2 cut(s) 283, 737
BciT130I CCWGG 3 cut(s) 68, 863, 1130
BclI TGATCA 2 cut(s) 525, 838
BcnI CCSGG 2 cut(s) 319, 577
BcoDI GTCTC 4 cut(s) 326, 354, 516, 849
BfaI CTAG 5 cut(s) 60, 248, 336, 459, 614
BfmI CTRYAG 1 cut(s) 803
BfoI RGCGCY 1 cut(s) 594
BisI GCNGC 8 cut(s) 129, 288, 490, 589, 750, 803, 806, 1285
BlsI GCNGC 8 cut(s) 130, 289, 491, 590, 751, 804, 807, 1286
Bme1390I CCNGG 5 cut(s) 68, 319, 577, 863, 1130
BmeT110I CYCGRG 1 cut(s) 875
BmgT120I GGNCC 2 cut(s) 959, 1168
BmiI GGNNCC 3 cut(s) 65, 960, 1386
BmrFI CCNGG 5 cut(s) 68, 319, 577, 863, 1130
BmsI GCATC 4 cut(s) 575, 688, 817, 919
BpiI GAAGAC 2 cut(s) 1152, 1188
Bpu10I CCTNAGC 1 cut(s) 629
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 2 cut(s) 319, 577
Bsa29I ATCGAT 1 cut(s) 1202
BsaAI YACGTR 1 cut(s) 1048
BsaBI GATNNNNATC 1 cut(s) 870
BsaHI GRCGYC 2 cut(s) 236, 1149
BsaJI CCNNGG 4 cut(s) 54, 618, 1129, 1377
BsaWI WCCGGW 2 cut(s) 695, 1051
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 1 cut(s) 60
Bse1I ACTGG 1 cut(s) 765
Bse8I GATNNNNATC 1 cut(s) 870
BseBI CCWGG 3 cut(s) 68, 863, 1130
BseCI ATCGAT 1 cut(s) 1202
BseDI CCNNGG 4 cut(s) 54, 618, 1129, 1377
BseGI GGATG 7 cut(s) 36, 43, 50, 585, 590, 744, 1248
BseJI GATNNNNATC 1 cut(s) 870
BseLI CCNNNNNNNGG 1 cut(s) 60
BseMII CTCAG 8 cut(s) 270, 529, 620, 660, 1024, 1127, 1208, 1326
BseNI ACTGG 1 cut(s) 765
BseRI GAGGAG 3 cut(s) 344, 759, 1048
BseX3I CGGCCG 1 cut(s) 750
BseXI GCAGC 5 cut(s) 299, 501, 600, 789, 817
BseYI CCCAGC 1 cut(s) 1091
BsgI GTGCAG 1 cut(s) 441
Bsh1236I CGCG 1 cut(s) 1304
Bsh1285I CGRYCG 2 cut(s) 753, 1258
BshFI GGCC 5 cut(s) 141, 752, 961, 1157, 1169
BshVI ATCGAT 1 cut(s) 1202
BsiEI CGRYCG 2 cut(s) 753, 1258
BsiHKAI GWGCWC 1 cut(s) 955
BsiHKCI CYCGRG 1 cut(s) 875
BsiSI CCGG 6 cut(s) 318, 576, 594, 696, 924, 1052
BslFI GGGAC 1 cut(s) 486
BslI CCNNNNNNNGG 1 cut(s) 60
BsmAI GTCTC 4 cut(s) 326, 354, 516, 849
BsmBI CGTCTC 2 cut(s) 354, 516
BsmFI GGGAC 1 cut(s) 486
BsmI GAATGC 2 cut(s) 290, 339
BsnI GGCC 5 cut(s) 141, 752, 961, 1157, 1169
BsoBI CYCGRG 1 cut(s) 875
Bsp1286I GDGCHC 1 cut(s) 955
Bsp1407I TGTACA 1 cut(s) 1173
Bsp143I GATC 6 cut(s) 63, 303, 525, 838, 871, 1255
Bsp19I CCATGG 2 cut(s) 54, 1377
BspACI CCGC 7 cut(s) 105, 128, 163, 749, 1103, 1284, 1304
BspANI GGCC 5 cut(s) 141, 752, 961, 1157, 1169
BspCNI CTCAG 8 cut(s) 271, 528, 621, 661, 1023, 1128, 1209, 1327
BspDI ATCGAT 1 cut(s) 1202
BspFNI CGCG 1 cut(s) 1304
BspLI GGNNCC 3 cut(s) 65, 960, 1386
BspMAI CTGCAG 1 cut(s) 807
BspPI GGATC 4 cut(s) 58, 71, 298, 866
BsrBI CCGCTC 3 cut(s) 749, 1103, 1284
BsrGI TGTACA 1 cut(s) 1173
BsrI ACTGG 1 cut(s) 765
BssECI CCNNGG 4 cut(s) 54, 618, 1129, 1377
BssMI GATC 6 cut(s) 63, 303, 525, 838, 871, 1255
BssNI GRCGYC 2 cut(s) 236, 1149
BssSI CACGAG 1 cut(s) 638
BssT1I CCWWGG 3 cut(s) 54, 618, 1377
Bst2BI CACGAG 1 cut(s) 638
Bst2UI CCWGG 3 cut(s) 68, 863, 1130
Bst4CI ACNGT 2 cut(s) 510, 708
Bst6I CTCTTC 1 cut(s) 390
BstACI GRCGYC 2 cut(s) 236, 1149
BstAUI TGTACA 1 cut(s) 1173
BstBAI YACGTR 1 cut(s) 1048
BstC8I GCNNGC 3 cut(s) 139, 358, 362
BstDEI CTNAG 8 cut(s) 279, 515, 629, 669, 1010, 1136, 1217, 1335
BstDSI CCRYGG 2 cut(s) 54, 1377
BstF5I GGATG 7 cut(s) 36, 43, 50, 585, 590, 744, 1248
BstFNI CGCG 1 cut(s) 1304
BstH2I RGCGCY 1 cut(s) 594
BstHHI GCGC 3 cut(s) 287, 593, 1304
BstKTI GATC 6 cut(s) 66, 306, 528, 841, 874, 1258
BstMAI GTCTC 4 cut(s) 326, 354, 516, 849
BstMBI GATC 6 cut(s) 63, 303, 525, 838, 871, 1255
BstMCI CGRYCG 2 cut(s) 753, 1258
BstMWI GCNNNNNNNGC 4 cut(s) 134, 929, 938, 1340
BstNI CCWGG 3 cut(s) 68, 863, 1130
BstNSI RCATGY 2 cut(s) 117, 1091
BstSCI CCNGG 5 cut(s) 66, 317, 575, 861, 1128
BstSFI CTRYAG 1 cut(s) 803
BstUI CGCG 1 cut(s) 1304
BstV1I GCAGC 5 cut(s) 299, 501, 600, 789, 817
BstV2I GAAGAC 2 cut(s) 1152, 1188
BstX2I RGATCY 3 cut(s) 63, 303, 871
BstYI RGATCY 3 cut(s) 63, 303, 871
BstZI CGGCCG 1 cut(s) 750
Bsu15I ATCGAT 1 cut(s) 1202
BsuRI GGCC 5 cut(s) 141, 752, 961, 1157, 1169
BsuTUI ATCGAT 1 cut(s) 1202
BtgI CCRYGG 2 cut(s) 54, 1377
BtsCI GGATG 7 cut(s) 36, 43, 50, 585, 590, 744, 1248
BtsI GCAGTG 2 cut(s) 108, 414
BtsIMutI CAGTG 3 cut(s) 108, 414, 666
Cac8I GCNNGC 3 cut(s) 139, 358, 362
CaiI CAGNNNCTG 1 cut(s) 278
CfoI GCGC 3 cut(s) 287, 593, 1304
Cfr13I GGNCC 2 cut(s) 959, 1168
ClaI ATCGAT 1 cut(s) 1202
Csp6I GTAC 5 cut(s) 691, 704, 1049, 1174, 1210
CviQI GTAC 5 cut(s) 691, 704, 1049, 1174, 1210
DdeI CTNAG 8 cut(s) 279, 515, 629, 669, 1010, 1136, 1217, 1335
DpnI GATC 6 cut(s) 65, 305, 527, 840, 873, 1257
DpnII GATC 6 cut(s) 63, 303, 525, 838, 871, 1255
EaeI YGGCCR 1 cut(s) 750
EagI CGGCCG 1 cut(s) 750
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
EclXI CGGCCG 1 cut(s) 750
Eco130I CCWWGG 3 cut(s) 54, 618, 1377
Eco147I AGGCCT 2 cut(s) 141, 1157
Eco32I GATATC 1 cut(s) 900
Eco52I CGGCCG 1 cut(s) 750
Eco88I CYCGRG 1 cut(s) 875
EcoRII CCWGG 3 cut(s) 66, 861, 1128
EcoRV GATATC 1 cut(s) 900
EcoT14I CCWWGG 3 cut(s) 54, 618, 1377
EcoT22I ATGCAT 1 cut(s) 934
ErhI CCWWGG 3 cut(s) 54, 618, 1377
Esp3I CGTCTC 2 cut(s) 354, 516
FaqI GGGAC 1 cut(s) 486
FauI CCCGC 1 cut(s) 112
FbaI TGATCA 2 cut(s) 525, 838
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 8 cut(s) 129, 288, 490, 589, 750, 803, 806, 1285
FokI GGATG 7 cut(s) 23, 30, 37, 592, 597, 751, 1255
Fsp4HI GCNGC 8 cut(s) 129, 288, 490, 589, 750, 803, 806, 1285
FspBI CTAG 5 cut(s) 60, 248, 336, 459, 614
GlaI GCGC 3 cut(s) 286, 592, 1303
GluI GCNGC 8 cut(s) 129, 288, 490, 589, 750, 803, 806, 1285
GsaI CCCAGC 1 cut(s) 1095
HaeII RGCGCY 1 cut(s) 594
HaeIII GGCC 5 cut(s) 141, 752, 961, 1157, 1169
HapII CCGG 6 cut(s) 318, 576, 594, 696, 924, 1052
HhaI GCGC 3 cut(s) 287, 593, 1304
Hin1I GRCGYC 2 cut(s) 236, 1149
Hin6I GCGC 3 cut(s) 285, 591, 1302
HinP1I GCGC 3 cut(s) 285, 591, 1302
HindIII AAGCTT 2 cut(s) 562, 1065
HinfI GANTC 2 cut(s) 772, 1042
HpaII CCGG 6 cut(s) 318, 576, 594, 696, 924, 1052
Hpy166II GTNNAC 3 cut(s) 193, 637, 693
Hpy188III TCNNGA 7 cut(s) 263, 301, 370, 638, 776, 875, 1279
Hpy8I GTNNAC 3 cut(s) 193, 637, 693
Hpy99I CGWCG 3 cut(s) 101, 238, 1154
HpyAV CCTTC 3 cut(s) 269, 1233, 1243
HpyCH4III ACNGT 2 cut(s) 510, 708
HpyCH4IV ACGT 6 cut(s) 75, 236, 255, 1047, 1149, 1347
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 929, 938, 1340
HpyF3I CTNAG 8 cut(s) 279, 515, 629, 669, 1010, 1136, 1217, 1335
HpySE526I ACGT 6 cut(s) 75, 236, 255, 1047, 1149, 1347
Hsp92I GRCGYC 2 cut(s) 236, 1149
HspAI GCGC 3 cut(s) 285, 591, 1302
Ksp22I TGATCA 2 cut(s) 525, 838
Kzo9I GATC 6 cut(s) 63, 303, 525, 838, 871, 1255
LmnI GCTCC 4 cut(s) 746, 982, 1281, 1331
Lsp1109I GCAGC 5 cut(s) 299, 501, 600, 789, 817
LweI GCATC 4 cut(s) 575, 688, 817, 919
MaeI CTAG 5 cut(s) 60, 248, 336, 459, 614
MaeII ACGT 6 cut(s) 75, 236, 255, 1047, 1149, 1347
MaeIII GTNAC 3 cut(s) 203, 1220, 1295
MalI GATC 6 cut(s) 65, 305, 527, 840, 873, 1257
MbiI CCGCTC 3 cut(s) 749, 1103, 1284
MboI GATC 6 cut(s) 63, 303, 525, 838, 871, 1255
MboII GAAGA 6 cut(s) 149, 407, 1051, 1157, 1188, 1258
MflI RGATCY 3 cut(s) 63, 303, 871
MhlI GDGCHC 1 cut(s) 955
MluCI AATT 6 cut(s) 180, 383, 465, 685, 724, 1269
MmeI TCCRAC 2 cut(s) 372, 749
Mph1103I ATGCAT 1 cut(s) 934
MroXI GAANNNNTTC 2 cut(s) 75, 1067
MseI TTAA 1 cut(s) 1059
MslI CAYNNNNRTG 2 cut(s) 852, 1204
MspI CCGG 6 cut(s) 318, 576, 594, 696, 924, 1052
MspR9I CCNGG 5 cut(s) 68, 319, 577, 863, 1130
Mva1269I GAATGC 2 cut(s) 290, 339
MvaI CCWGG 3 cut(s) 68, 863, 1130
MvnI CGCG 1 cut(s) 1304
MwoI GCNNNNNNNGC 4 cut(s) 134, 929, 938, 1340
NciI CCSGG 2 cut(s) 319, 577
NcoI CCATGG 2 cut(s) 54, 1377
NdeII GATC 6 cut(s) 63, 303, 525, 838, 871, 1255
NlaIV GGNNCC 3 cut(s) 65, 960, 1386
NsiI ATGCAT 1 cut(s) 934
NspI RCATGY 2 cut(s) 117, 1091
PceI AGGCCT 2 cut(s) 141, 1157
PcsI WCGNNNNNNNCGW 1 cut(s) 1199
PctI GAATGC 2 cut(s) 290, 339
PdmI GAANNNNTTC 2 cut(s) 75, 1067
PfeI GAWTC 2 cut(s) 772, 1042
PflFI GACNNNGTC 2 cut(s) 95, 235
PfoI TCCNGGA 3 cut(s) 66, 575, 861
PkrI GCNGC 8 cut(s) 130, 289, 491, 590, 751, 804, 807, 1286
Ple19I CGATCG 1 cut(s) 1258
Ppu21I YACGTR 1 cut(s) 1048
Psp6I CCWGG 3 cut(s) 66, 861, 1128
PspFI CCCAGC 1 cut(s) 1091
PspGI CCWGG 3 cut(s) 66, 861, 1128
PspN4I GGNNCC 3 cut(s) 65, 960, 1386
PspPI GGNCC 2 cut(s) 959, 1168
PstI CTGCAG 1 cut(s) 807
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 3 cut(s) 63, 303, 871
PsyI GACNNNGTC 2 cut(s) 95, 235
PvuI CGATCG 1 cut(s) 1258
RsaI GTAC 5 cut(s) 692, 705, 1050, 1175, 1211
RsaNI GTAC 5 cut(s) 691, 704, 1049, 1174, 1210
RseI CAYNNNNRTG 2 cut(s) 852, 1204
SaqAI TTAA 1 cut(s) 1059
SatI GCNGC 8 cut(s) 129, 288, 490, 589, 750, 803, 806, 1285
Sau3AI GATC 6 cut(s) 63, 303, 525, 838, 871, 1255
Sau96I GGNCC 2 cut(s) 959, 1168
ScrFI CCNGG 5 cut(s) 68, 319, 577, 863, 1130
SduI GDGCHC 1 cut(s) 955
SfaNI GCATC 4 cut(s) 575, 688, 817, 919
SfcI CTRYAG 1 cut(s) 803
SmiMI CAYNNNNRTG 2 cut(s) 852, 1204
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 6 cut(s) 180, 383, 465, 685, 724, 1269
SseBI AGGCCT 2 cut(s) 141, 1157
SsiI CCGC 7 cut(s) 105, 128, 163, 749, 1103, 1284, 1304
SspMI CTAG 5 cut(s) 60, 248, 336, 459, 614
StuI AGGCCT 2 cut(s) 141, 1157
StyD4I CCNGG 5 cut(s) 66, 317, 575, 861, 1128
StyI CCWWGG 3 cut(s) 54, 618, 1377
TaaI ACNGT 2 cut(s) 510, 708
TaiI ACGT 6 cut(s) 78, 239, 258, 1050, 1152, 1350
TaqI TCGA 7 cut(s) 99, 896, 991, 1004, 1071, 1152, 1202
TasI AATT 6 cut(s) 180, 383, 465, 685, 724, 1269
TatI WGTACW 1 cut(s) 1173
TauI GCSGC 3 cut(s) 131, 752, 1287
TfiI GAWTC 2 cut(s) 772, 1042
Tru1I TTAA 1 cut(s) 1059
Tru9I TTAA 1 cut(s) 1059
TscAI CASTG 3 cut(s) 115, 414, 673
TseI GCWGC 5 cut(s) 287, 489, 588, 802, 805
TspDTI ATGAA 5 cut(s) 24, 68, 1188, 1259, 1359
TspGWI ACGGA 1 cut(s) 336
TspRI CASTG 3 cut(s) 115, 414, 673
Tth111I GACNNNGTC 2 cut(s) 95, 235
XapI RAATTY 1 cut(s) 180
XceI RCATGY 2 cut(s) 117, 1091
XmiI GTMKAC 1 cut(s) 192
XmnI GAANNNNTTC 2 cut(s) 75, 1067
XspI CTAG 5 cut(s) 60, 248, 336, 459, 614
ZraI GACGTC 2 cut(s) 237, 1150
Zsp2I ATGCAT 1 cut(s) 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.