AT5G54500

quinone reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013815
Physical Location & Seq
Forward (+)
22124390 .. 22126618
2229 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54500.1

Sequence Viewer

Length: 615 bp
ATGGCGACTAAAGTATATATCGTGTACTACTCTATGTATGGTCATGTTGAGAAATTGGCTGAAGAAATAAGGAAAGGAGCTGCTTCTGTTGAAGGTGTTGAAGCTAAACTATGGCAGGTACCAGAGACGCTTCACGAAGAGGCACTCTCTAAGATGAGCGCACCACCAAAGAGTGAATCCCCAATCATAACCCCGAATGAGCTAGCTGAAGCTGATGGGTTTGTCTTTGGTTTCCCAACAAGATTTGGTATGATGGCTGCTCAGTTCAAAGCCTTTTTGGATGCAACCGGTGGACTCTGGAGGGCTCAGGCACTCGCCGGTAAACCAGCTGGTATCTTCTACAGCACTGGCTCTCAAGGTGGTGGCCAAGAAACCACAGCATTGACGGCAATAACTCAGCTGGTTCACCACGGGATGTTATTTGTCCCAATCGGTTACACATTTGGCGCGGGAATGTTCGAAATGGAGAATGTGAAAGGTGGAAGCCCATATGGAGCTGGAACATTTGCAGGAGACGGTTCAAGGCAGCCAACAGAGCTGGAGCTACAGCAAGCATTTCACCAAGGCCAGTACATTGCCAGCATCACCAAGAAGCTCAAGGGATCTACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

204

Amino Acids

21.8

Weight (kDa)

5.96

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Flavodoxin_1 PF00258 7 - 138 3.8e-10 Flavodoxin
FMN_red PF03358 17 - 145 1e-13 NADPH-dependent FMN reductase
Flavodoxin_2 PF02525 17 - 127 6.1e-06 Flavodoxin-like fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013815)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54500
fragaria_vesca FvH4_3g03210 FvH4_3g03210
rosa_chinensis RchiOBHm_Chr5g0004841
rosa_laevigata RLG00000031266
rosa_multiflora Rmu_sc0010871.1_g000002
rosa_roxburghii Rroxscaffold_1G00070770
rosa_rugosa Rorug04G0414400 Rorug04G0414400 Rorug04G0414500 Rorug04G0414600 Rorug04G0414700.1
rosa_samantha Rh5AG042200 Rh5BG041900 Rh5CG045700 Rh5DG042300
rosa_wichuraiana Rw5G004080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 106
Acc65I GGTACC 1 cut(s) 118
AccB1I GGYRCC 1 cut(s) 118
AccII CGCG 1 cut(s) 449
AciI CCGC 1 cut(s) 449
AclWI GGATC 1 cut(s) 610
AcoI YGGCCR 1 cut(s) 364
AcuI CTGAAG 2 cut(s) 81, 228
AfaI GTAC 3 cut(s) 26, 120, 572
AgeI ACCGGT 1 cut(s) 287
AgsI TTSAA 4 cut(s) 92, 101, 268, 522
Alw26I GTCTC 2 cut(s) 119, 507
AlwI GGATC 1 cut(s) 610
AoxI GGCC 2 cut(s) 364, 565
ApeKI GCWGC 3 cut(s) 80, 257, 526
AsiGI ACCGGT 1 cut(s) 287
Asp718I GGTACC 1 cut(s) 118
AspLEI GCGC 2 cut(s) 161, 449
AsuHPI GGTGA 3 cut(s) 398, 551, 577
AsuII TTCGAA 1 cut(s) 459
AsuNHI GCTAGC 1 cut(s) 202
BalI TGGCCA 1 cut(s) 366
BanI GGYRCC 1 cut(s) 118
BanII GRGCYC 1 cut(s) 307
BbvI GCAGC 3 cut(s) 67, 244, 538
BccI CCATC 2 cut(s) 209, 247
BceAI ACGGC 1 cut(s) 402
BcoDI GTCTC 2 cut(s) 119, 507
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 2 cut(s) 340, 545
BfuAI ACCTGC 1 cut(s) 106
BisI GCNGC 3 cut(s) 81, 258, 527
BlsI GCNGC 3 cut(s) 82, 259, 528
BmiI GGNNCC 1 cut(s) 120
BmsI GCATC 2 cut(s) 271, 591
BmtI GCTAGC 1 cut(s) 206
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpmI CTGGAG 2 cut(s) 319, 560
Bpu10I CCTNAGC 1 cut(s) 306
Bpu14I TTCGAA 1 cut(s) 459
BpuEI CTTGAG 2 cut(s) 339, 581
BsaJI CCNNGG 2 cut(s) 409, 562
BsaWI WCCGGW 1 cut(s) 287
Bse118I RCCGGY 2 cut(s) 287, 317
Bse1I ACTGG 2 cut(s) 352, 568
Bse3DI GCAATG 1 cut(s) 573
BseDI CCNNGG 2 cut(s) 409, 562
BseGI GGATG 2 cut(s) 286, 420
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 3 cut(s) 275, 320, 410
BseNI ACTGG 2 cut(s) 352, 568
BseXI GCAGC 3 cut(s) 67, 244, 538
Bsh1236I CGCG 1 cut(s) 449
BshFI GGCC 2 cut(s) 366, 567
BshNI GGYRCC 1 cut(s) 118
BshTI ACCGGT 1 cut(s) 287
BsiSI CCGG 2 cut(s) 288, 318
BslFI GGGAC 1 cut(s) 410
BsmAI GTCTC 2 cut(s) 119, 507
BsmBI CGTCTC 2 cut(s) 119, 507
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 2 cut(s) 366, 567
Bsp119I TTCGAA 1 cut(s) 459
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 1 cut(s) 602
BspACI CCGC 1 cut(s) 449
BspANI GGCC 2 cut(s) 366, 567
BspCNI CTCAG 3 cut(s) 274, 319, 409
BspFNI CGCG 1 cut(s) 449
BspLI GGNNCC 1 cut(s) 120
BspMI ACCTGC 1 cut(s) 106
BspOI GCTAGC 1 cut(s) 206
BspPI GGATC 1 cut(s) 610
BspT104I TTCGAA 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 573
BsrFI RCCGGY 2 cut(s) 287, 317
BsrI ACTGG 2 cut(s) 352, 568
BssAI RCCGGY 2 cut(s) 287, 317
BssECI CCNNGG 2 cut(s) 409, 562
BssMI GATC 1 cut(s) 602
BssT1I CCWWGG 1 cut(s) 562
Bst4CI ACNGT 1 cut(s) 518
Bst6I CTCTTC 1 cut(s) 132
BstBI TTCGAA 1 cut(s) 459
BstC8I GCNNGC 3 cut(s) 204, 552, 580
BstDEI CTNAG 5 cut(s) 150, 261, 306, 396, 612
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 2 cut(s) 286, 420
BstFNI CGCG 1 cut(s) 449
BstHHI GCGC 2 cut(s) 161, 449
BstKTI GATC 1 cut(s) 605
BstMAI GTCTC 2 cut(s) 119, 507
BstMBI GATC 1 cut(s) 602
BstMWI GCNNNNNNNGC 2 cut(s) 386, 535
BstSFI CTRYAG 2 cut(s) 340, 545
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 3 cut(s) 67, 244, 538
BstX2I RGATCY 1 cut(s) 602
BstYI RGATCY 1 cut(s) 602
BsuRI GGCC 2 cut(s) 366, 567
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 2 cut(s) 286, 420
BtsIMutI CAGTG 1 cut(s) 345
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 3 cut(s) 204, 552, 580
CfoI GCGC 2 cut(s) 161, 449
Cfr10I RCCGGY 2 cut(s) 287, 317
CseI GACGC 1 cut(s) 136
Csp6I GTAC 3 cut(s) 25, 119, 571
CspAI ACCGGT 1 cut(s) 287
CviAII CATG 1 cut(s) 44
CviQI GTAC 3 cut(s) 25, 119, 571
DdeI CTNAG 5 cut(s) 150, 261, 306, 396, 612
DpnI GATC 1 cut(s) 604
DpnII GATC 1 cut(s) 602
EaeI YGGCCR 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 562
Eco24I GRGCYC 1 cut(s) 307
Eco57I CTGAAG 2 cut(s) 81, 228
EcoT14I CCWWGG 1 cut(s) 562
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 562
Esp3I CGTCTC 2 cut(s) 119, 507
FaeI CATG 1 cut(s) 47
FaqI GGGAC 1 cut(s) 410
FatI CATG 1 cut(s) 43
FauI CCCGC 1 cut(s) 442
FauNDI CATATG 1 cut(s) 490
Fnu4HI GCNGC 3 cut(s) 81, 258, 527
FokI GGATG 2 cut(s) 293, 427
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 3 cut(s) 81, 258, 527
FspBI CTAG 1 cut(s) 203
GlaI GCGC 2 cut(s) 160, 448
GluI GCNGC 3 cut(s) 81, 258, 527
GsuI CTGGAG 2 cut(s) 319, 560
HaeIII GGCC 2 cut(s) 366, 567
HapII CCGG 2 cut(s) 288, 318
HgaI GACGC 1 cut(s) 136
HhaI GCGC 2 cut(s) 161, 449
Hin1II CATG 1 cut(s) 47
Hin6I GCGC 2 cut(s) 159, 447
HinP1I GCGC 2 cut(s) 159, 447
HinfI GANTC 2 cut(s) 176, 294
HpaII CCGG 2 cut(s) 288, 318
HphI GGTGA 3 cut(s) 398, 551, 577
Hpy166II GTNNAC 4 cut(s) 25, 293, 323, 406
Hpy188III TCNNGA 2 cut(s) 134, 298
Hpy8I GTNNAC 4 cut(s) 25, 293, 323, 406
HpyAV CCTTC 1 cut(s) 86
HpyCH4III ACNGT 1 cut(s) 518
HpyCH4V TGCA 2 cut(s) 284, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 535
HpyF3I CTNAG 5 cut(s) 150, 261, 306, 396, 612
Hsp92II CATG 1 cut(s) 47
HspAI GCGC 2 cut(s) 159, 447
KpnI GGTACC 1 cut(s) 122
Kzo9I GATC 1 cut(s) 602
LmnI GCTCC 3 cut(s) 77, 494, 541
Lsp1109I GCAGC 3 cut(s) 67, 244, 538
LweI GCATC 2 cut(s) 271, 591
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 1 cut(s) 434
MalI GATC 1 cut(s) 604
MboI GATC 1 cut(s) 602
MboII GAAGA 3 cut(s) 74, 149, 328
MflI RGATCY 1 cut(s) 602
MhlI GDGCHC 1 cut(s) 307
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 1 cut(s) 53
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 1 cut(s) 288
MnlI CCTC 2 cut(s) 133, 294
Mox20I TGGCCA 1 cut(s) 366
MscI TGGCCA 1 cut(s) 366
Msp20I TGGCCA 1 cut(s) 366
MspA1I CMGCKG 2 cut(s) 329, 400
MspI CCGG 2 cut(s) 288, 318
MvnI CGCG 1 cut(s) 449
MwoI GCNNNNNNNGC 2 cut(s) 386, 535
NdeI CATATG 1 cut(s) 490
NdeII GATC 1 cut(s) 602
NheI GCTAGC 1 cut(s) 202
NlaIII CATG 1 cut(s) 47
NlaIV GGNNCC 1 cut(s) 120
NspV TTCGAA 1 cut(s) 459
PfeI GAWTC 1 cut(s) 176
PinAI ACCGGT 1 cut(s) 287
PkrI GCNGC 3 cut(s) 82, 259, 528
PleI GAGTC 1 cut(s) 288
PpsI GAGTC 1 cut(s) 288
PspN4I GGNNCC 1 cut(s) 120
PsuI RGATCY 1 cut(s) 602
PvuII CAGCTG 2 cut(s) 329, 400
RsaI GTAC 3 cut(s) 26, 120, 572
RsaNI GTAC 3 cut(s) 25, 119, 571
SatI GCNGC 3 cut(s) 81, 258, 527
Sau3AI GATC 1 cut(s) 602
SchI GAGTC 1 cut(s) 288
SduI GDGCHC 1 cut(s) 307
SfaNI GCATC 2 cut(s) 271, 591
SfcI CTRYAG 2 cut(s) 340, 545
SfuI TTCGAA 1 cut(s) 459
SmlI CTYRAG 2 cut(s) 354, 596
SmoI CTYRAG 2 cut(s) 354, 596
Sse9I AATT 1 cut(s) 53
SsiI CCGC 1 cut(s) 449
SspMI CTAG 1 cut(s) 203
StyI CCWWGG 1 cut(s) 562
TaaI ACNGT 1 cut(s) 518
TaqI TCGA 1 cut(s) 459
TasI AATT 1 cut(s) 53
TatI WGTACW 2 cut(s) 24, 570
TfiI GAWTC 1 cut(s) 176
TscAI CASTG 1 cut(s) 352
TseI GCWGC 3 cut(s) 80, 257, 526
TspRI CASTG 1 cut(s) 352
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.