Rorug04G0414600

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0013815
Physical Location & Seq
Reverse (-)
57617119 .. 57618654
1536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0414600.1

Sequence Viewer

Length: 864 bp
ATGACCCAAATCCCTTTGTTCCTCCATCAGATGGTTCCAGTGACGTCCGATGAGCAAGGCAGAAATAGCAGTGATTGTTTTTATAGTGATAAACAACCGGCAGCTGCAGCTCCTCAGCGAAGTTCCCGATGGAATCCAACCCCGGAGCAGATAATGCACCTGGAGGAGCTCTATCGATCTGGGATCAAGACACCGACGGCCCAACAAATCCGACAGATGACTGCACGGCTTCGAAACTATGGGAGGGTTGAAGGCAAGAATGTATTCTACTGGTTTCAGAACCACCGAGCCAGGGAGAGGCAGAAGAGACGCCGTGAACTCATGTCCATGTATCAGTCTGACTCTAAACAACAACAACAACAAACTTTGACTAACAACACAATTAGCAGTCTCCACGACAAAGAATCTGCAGGAGGTGACATTGTTCGTTGTCTTGAAGTTGAGCAGCAGAATAGGCGGACACTACCAAACTTAACTGTTCTTTCCGATCAGGAATCATCTTCAGTACTGCAAGAGAGGATGATGAGAATGGTGAAAAGCAATTCCTCCAAGCTACTTGTGGAACAATCCGATTCACCTGGATCGACCTCAAACACAAACGCGTCAGCTACAGTCATTTACACAAAGCTCTTGGACTTCCATCACTATTATGATTATGATTATGATTATGGTATGGTATTGACCACACCCTGTATTTACAAAGAATTCAAAGAAGAAGAAGAAGAAAGCAGAGAAACTCAAACTCTGGAGCTCTTTCCACTCGAGAGCGACAATCTTAAAGGTGCTAAGGATACCAAATTACCTATGAGCAGAACCACAAGCACAGACACTACTTCAGATTTCATGGCAGACCAATATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

287

Amino Acids

33.36

Weight (kDa)

5.93

Isoelectric Point (pI)

65.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 40 - 101 6.3e-14 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013815)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54500
fragaria_vesca FvH4_3g03210 FvH4_3g03210
rosa_chinensis RchiOBHm_Chr5g0004841
rosa_laevigata RLG00000031266
rosa_multiflora Rmu_sc0010871.1_g000002
rosa_roxburghii Rroxscaffold_1G00070770
rosa_rugosa Rorug04G0414400 Rorug04G0414400 Rorug04G0414500 Rorug04G0414600 Rorug04G0414700.1
rosa_samantha Rh5AG042200 Rh5BG041900 Rh5CG045700 Rh5DG042300
rosa_wichuraiana Rw5G004080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 47
AccB7I CCANNNNNTGG 1 cut(s) 31
AccII CGCG 1 cut(s) 602
AciI CCGC 1 cut(s) 457
AclWI GGATC 2 cut(s) 191, 589
AcsI RAATTY 1 cut(s) 704
AcuI CTGAAG 2 cut(s) 486, 819
AcyI GRCGYC 2 cut(s) 44, 310
AfaI GTAC 1 cut(s) 507
AfiI CCNNNNNNNGG 3 cut(s) 31, 292, 297
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 3 cut(s) 251, 437, 709
AjnI CCWGG 3 cut(s) 159, 290, 577
AluBI AGCT 7 cut(s) 104, 110, 169, 553, 608, 628, 751
AluI AGCT 7 cut(s) 104, 110, 169, 553, 608, 628, 751
Alw21I GWGCWC 2 cut(s) 171, 753
Alw26I GTCTC 2 cut(s) 301, 395
AlwI GGATC 2 cut(s) 191, 589
Ama87I CYCGRG 1 cut(s) 761
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 4 cut(s) 101, 104, 107, 445
ApoI RAATTY 1 cut(s) 704
Asp700I GAANNNNTTC 1 cut(s) 263
AspS9I GGNCC 1 cut(s) 199
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 3 cut(s) 428, 544, 567
AsuII TTCGAA 1 cut(s) 232
AvaI CYCGRG 1 cut(s) 761
BanII GRGCYC 2 cut(s) 171, 753
Bbv12I GWGCWC 2 cut(s) 171, 753
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 4 cut(s) 91, 113, 119, 457
BccI CCATC 4 cut(s) 25, 33, 123, 648
BceAI ACGGC 3 cut(s) 213, 242, 297
BciT130I CCWGG 3 cut(s) 161, 292, 579
BciVI GTATCC 1 cut(s) 784
BcnI CCSGG 1 cut(s) 143
BcoDI GTCTC 2 cut(s) 301, 395
BfmI CTRYAG 3 cut(s) 105, 408, 609
BfuI GTATCC 1 cut(s) 784
BisI GCNGC 4 cut(s) 102, 105, 108, 446
BlsI GCNGC 4 cut(s) 103, 106, 109, 447
BmcAI AGTACT 1 cut(s) 507
Bme1390I CCNGG 4 cut(s) 143, 161, 292, 579
BmeT110I CYCGRG 1 cut(s) 761
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 4 cut(s) 143, 161, 292, 579
BpmI CTGGAG 2 cut(s) 182, 767
Bpu10I CCTNAGC 2 cut(s) 114, 786
Bpu14I TTCGAA 1 cut(s) 232
BpuMI CCSGG 1 cut(s) 143
Bsa29I ATCGAT 1 cut(s) 175
BsaHI GRCGYC 2 cut(s) 44, 310
BsaJI CCNNGG 2 cut(s) 141, 291
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 292, 297
Bse118I RCCGGY 1 cut(s) 97
Bse1I ACTGG 2 cut(s) 38, 275
BseBI CCWGG 3 cut(s) 161, 292, 579
BseCI ATCGAT 1 cut(s) 175
BseDI CCNNGG 2 cut(s) 141, 291
BseGI GGATG 1 cut(s) 525
BseLI CCNNNNNNNGG 3 cut(s) 31, 292, 297
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 2 cut(s) 38, 275
BseRI GAGGAG 2 cut(s) 102, 179
BseXI GCAGC 4 cut(s) 91, 113, 119, 457
BsgI GTGCAG 1 cut(s) 207
Bsh1236I CGCG 1 cut(s) 602
BshFI GGCC 1 cut(s) 200
BshVI ATCGAT 1 cut(s) 175
BsiHKAI GWGCWC 2 cut(s) 171, 753
BsiHKCI CYCGRG 1 cut(s) 761
BsiSI CCGG 2 cut(s) 98, 143
BslI CCNNNNNNNGG 3 cut(s) 31, 292, 297
BsmAI GTCTC 2 cut(s) 301, 395
BsmBI CGTCTC 1 cut(s) 301
BsnI GGCC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 761
Bsp119I TTCGAA 1 cut(s) 232
Bsp1286I GDGCHC 2 cut(s) 171, 753
Bsp143I GATC 4 cut(s) 176, 183, 487, 581
BspACI CCGC 1 cut(s) 457
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 127
BspDI ATCGAT 1 cut(s) 175
BspFNI CGCG 1 cut(s) 602
BspLI GGNNCC 1 cut(s) 36
BspMAI CTGCAG 2 cut(s) 109, 412
BspPI GGATC 2 cut(s) 191, 589
BspT104I TTCGAA 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 97
BsrI ACTGG 2 cut(s) 38, 275
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 2 cut(s) 141, 291
BssMI GATC 4 cut(s) 176, 183, 487, 581
BssNI GRCGYC 2 cut(s) 44, 310
Bst2UI CCWGG 3 cut(s) 161, 292, 579
Bst4CI ACNGT 2 cut(s) 478, 613
Bst6I CTCTTC 1 cut(s) 299
BstACI GRCGYC 2 cut(s) 44, 310
BstAPI GCANNNNNTGC 1 cut(s) 154
BstBI TTCGAA 1 cut(s) 232
BstDEI CTNAG 2 cut(s) 114, 786
BstF5I GGATG 1 cut(s) 525
BstFNI CGCG 1 cut(s) 602
BstKTI GATC 4 cut(s) 179, 186, 490, 584
BstMAI GTCTC 2 cut(s) 301, 395
BstMBI GATC 4 cut(s) 176, 183, 487, 581
BstMWI GCNNNNNNNGC 4 cut(s) 66, 107, 154, 454
BstNI CCWGG 3 cut(s) 161, 292, 579
BstSCI CCNGG 4 cut(s) 141, 159, 290, 577
BstSFI CTRYAG 3 cut(s) 105, 408, 609
BstUI CGCG 1 cut(s) 602
BstV1I GCAGC 4 cut(s) 91, 113, 119, 457
Bsu15I ATCGAT 1 cut(s) 175
BsuI GTATCC 1 cut(s) 784
BsuRI GGCC 1 cut(s) 200
BsuTUI ATCGAT 1 cut(s) 175
BtsCI GGATG 1 cut(s) 525
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 45, 76
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 199
ClaI ATCGAT 1 cut(s) 175
CseI GACGC 2 cut(s) 318, 591
Csp6I GTAC 1 cut(s) 506
CviAII CATG 3 cut(s) 322, 328, 844
CviQI GTAC 1 cut(s) 506
DdeI CTNAG 2 cut(s) 114, 786
DpnI GATC 4 cut(s) 178, 185, 489, 583
DpnII GATC 4 cut(s) 176, 183, 487, 581
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
EciI GGCGGA 1 cut(s) 472
Ecl136II GAGCTC 2 cut(s) 169, 751
Eco24I GRGCYC 2 cut(s) 171, 753
Eco53kI GAGCTC 2 cut(s) 169, 751
Eco57I CTGAAG 2 cut(s) 486, 819
Eco88I CYCGRG 1 cut(s) 761
EcoICRI GAGCTC 2 cut(s) 169, 751
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 3 cut(s) 159, 290, 577
EcoT38I GRGCYC 2 cut(s) 171, 753
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 3 cut(s) 325, 331, 847
FatI CATG 3 cut(s) 321, 327, 843
Fnu4HI GCNGC 4 cut(s) 102, 105, 108, 446
FokI GGATG 1 cut(s) 532
FriOI GRGCYC 2 cut(s) 171, 753
Fsp4HI GCNGC 4 cut(s) 102, 105, 108, 446
GluI GCNGC 4 cut(s) 102, 105, 108, 446
GsuI CTGGAG 2 cut(s) 182, 767
HaeIII GGCC 1 cut(s) 200
HapII CCGG 2 cut(s) 98, 143
HgaI GACGC 2 cut(s) 318, 591
Hin1I GRCGYC 2 cut(s) 44, 310
Hin1II CATG 3 cut(s) 325, 331, 847
HinfI GANTC 5 cut(s) 133, 341, 404, 494, 572
HpaII CCGG 2 cut(s) 98, 143
HphI GGTGA 3 cut(s) 428, 544, 567
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 8 cut(s) 30, 49, 212, 279, 340, 487, 571, 838
Hpy188III TCNNGA 6 cut(s) 126, 187, 434, 491, 746, 763
Hpy8I GTNNAC 1 cut(s) 317
Hpy99I CGWCG 1 cut(s) 199
HpyAV CCTTC 1 cut(s) 245
HpyCH4III ACNGT 2 cut(s) 478, 613
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 5 cut(s) 107, 157, 224, 410, 511
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 107, 154, 454
HpyF3I CTNAG 2 cut(s) 114, 786
HpySE526I ACGT 1 cut(s) 44
Hsp92I GRCGYC 2 cut(s) 44, 310
Hsp92II CATG 3 cut(s) 325, 331, 847
Kzo9I GATC 4 cut(s) 176, 183, 487, 581
LmnI GCTCC 4 cut(s) 115, 145, 166, 748
Lsp1109I GCAGC 4 cut(s) 91, 113, 119, 457
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 2 cut(s) 40, 416
MalI GATC 4 cut(s) 178, 185, 489, 583
MboI GATC 4 cut(s) 176, 183, 487, 581
MboII GAAGA 6 cut(s) 316, 492, 725, 728, 731, 734
MhlI GDGCHC 2 cut(s) 171, 753
MluCI AATT 4 cut(s) 381, 541, 704, 797
MluI ACGCGT 1 cut(s) 600
MlyI GAGTC 1 cut(s) 335
MmeI TCCRAC 2 cut(s) 161, 235
MnlI CCTC 9 cut(s) 32, 123, 157, 237, 291, 407, 510, 556, 598
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 2 cut(s) 473, 777
MslI CAYNNNNRTG 2 cut(s) 326, 648
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 2 cut(s) 98, 143
MspR9I CCNGG 4 cut(s) 143, 161, 292, 579
MvaI CCWGG 3 cut(s) 161, 292, 579
MvnI CGCG 1 cut(s) 602
MwoI GCNNNNNNNGC 4 cut(s) 66, 107, 154, 454
NciI CCSGG 1 cut(s) 143
NdeII GATC 4 cut(s) 176, 183, 487, 581
NlaIII CATG 3 cut(s) 325, 331, 847
NlaIV GGNNCC 1 cut(s) 36
NmuCI GTSAC 2 cut(s) 40, 416
NspV TTCGAA 1 cut(s) 232
PaeR7I CTCGAG 1 cut(s) 761
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 4 cut(s) 133, 404, 494, 572
PflMI CCANNNNNTGG 1 cut(s) 31
PkrI GCNGC 4 cut(s) 103, 106, 109, 447
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
Psp124BI GAGCTC 2 cut(s) 171, 753
Psp6I CCWGG 3 cut(s) 159, 290, 577
PspGI CCWGG 3 cut(s) 159, 290, 577
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 1 cut(s) 199
PstI CTGCAG 2 cut(s) 109, 412
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 1 cut(s) 507
RsaNI GTAC 1 cut(s) 506
RseI CAYNNNNRTG 2 cut(s) 326, 648
SacI GAGCTC 2 cut(s) 171, 753
SaqAI TTAA 2 cut(s) 473, 777
SatI GCNGC 4 cut(s) 102, 105, 108, 446
Sau3AI GATC 4 cut(s) 176, 183, 487, 581
Sau96I GGNCC 1 cut(s) 199
ScaI AGTACT 1 cut(s) 507
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 4 cut(s) 143, 161, 292, 579
SduI GDGCHC 2 cut(s) 171, 753
SfcI CTRYAG 3 cut(s) 105, 408, 609
Sfr274I CTCGAG 1 cut(s) 761
SfuI TTCGAA 1 cut(s) 232
SlaI CTCGAG 1 cut(s) 761
SmiMI CAYNNNNRTG 2 cut(s) 326, 648
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 4 cut(s) 381, 541, 704, 797
SsiI CCGC 1 cut(s) 457
SspI AATATT 1 cut(s) 857
SstI GAGCTC 2 cut(s) 171, 753
StyD4I CCNGG 4 cut(s) 141, 159, 290, 577
TaaI ACNGT 2 cut(s) 478, 613
TaiI ACGT 1 cut(s) 47
TaqI TCGA 4 cut(s) 175, 232, 584, 762
TasI AATT 4 cut(s) 381, 541, 704, 797
TatI WGTACW 1 cut(s) 505
TfiI GAWTC 4 cut(s) 133, 404, 494, 572
Tru1I TTAA 2 cut(s) 473, 777
Tru9I TTAA 2 cut(s) 473, 777
TscAI CASTG 2 cut(s) 45, 76
TseFI GTSAC 2 cut(s) 40, 416
TseI GCWGC 4 cut(s) 101, 104, 107, 445
Tsp45I GTSAC 2 cut(s) 40, 416
TspDTI ATGAA 1 cut(s) 832
TspRI CASTG 2 cut(s) 45, 76
Van91I CCANNNNNTGG 1 cut(s) 31
XapI RAATTY 1 cut(s) 704
XcmI CCANNNNNNNNNTGG 1 cut(s) 556
XhoI CTCGAG 1 cut(s) 761
XmnI GAANNNNTTC 1 cut(s) 263
ZraI GACGTC 1 cut(s) 45
ZrmI AGTACT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.