Rh5DG042300

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013815
Physical Location & Seq
Reverse (-)
3142546 .. 3145517
2972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG042300.1

Sequence Viewer

Length: 543 bp
ATGGTGTATTACAATAAATTCTTGTATTCGGTAACCTGCTTCATGGTACCAGAAACACTGCAAGATGAGGTTCTTGGAAAGATGGGTGCGCCACCAAAGAGCGAAGTACCTATTATTGCACCCAATGAGCTTTCTGATGCCGATGGTTTACTATTTGGTTTCCCTACTAGATTTGGAATGATGGCTGCACAGTTCAAAGCATTTTTAGATGCAACCGGAGGTTTATGGAGGACACAACAGTTAGCAGGCAAACCTGCAGGAATTTTCTACAGCACTGGTTCTCAAGGAGGTGGACAAGAGACTACTGCCTTGACAGCCATCACTCAGCTTGTTCACCATGGAATGGTGTTTGTGCCAATTGGGTACACATTTGGAGCTGGCATGTTTGAGATGGAGCAGATCAAGGGTGGCAGCCCCTATGGTGCAGGAACTTATGCCGGGGATGGCACAAGACAACCTTCTGAACTCGAACTACAGCAAGCTTTCCACCAGGGAAAGTATTTCGCCAGCATTGCCAAGAAACTCAAGGGGACTACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

180

Amino Acids

19.3

Weight (kDa)

6.28

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMN_red PF03358 44 - 121 2.1e-11 NADPH-dependent FMN reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013815)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54500
fragaria_vesca FvH4_3g03210 FvH4_3g03210
rosa_chinensis RchiOBHm_Chr5g0004841
rosa_laevigata RLG00000031266
rosa_multiflora Rmu_sc0010871.1_g000002
rosa_roxburghii Rroxscaffold_1G00070770
rosa_rugosa Rorug04G0414400 Rorug04G0414400 Rorug04G0414500 Rorug04G0414600 Rorug04G0414700.1
rosa_samantha Rh5AG042200 Rh5BG041900 Rh5CG045700 Rh5DG042300
rosa_wichuraiana Rw5G004080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 44, 262
Acc65I GGTACC 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 46
AccB7I CCANNNNNTGG 1 cut(s) 343
AcsI RAATTY 2 cut(s) 17, 261
AfaI GTAC 3 cut(s) 48, 108, 365
AfiI CCNNNNNNNGG 1 cut(s) 343
AgsI TTSAA 1 cut(s) 196
AjnI CCWGG 1 cut(s) 489
AluBI AGCT 4 cut(s) 130, 328, 377, 482
AluI AGCT 4 cut(s) 130, 328, 377, 482
Alw26I GTCTC 1 cut(s) 293
ApeKI GCWGC 2 cut(s) 185, 411
ApoI RAATTY 2 cut(s) 17, 261
Asp718I GGTACC 1 cut(s) 46
AspLEI GCGC 1 cut(s) 91
AsuC2I CCSGG 1 cut(s) 439
AsuHPI GGTGA 1 cut(s) 326
BanI GGYRCC 1 cut(s) 46
BbvI GCAGC 2 cut(s) 172, 423
BccI CCATC 6 cut(s) 76, 137, 175, 326, 385, 437
BciT130I CCWGG 1 cut(s) 491
BcnI CCSGG 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 3 cut(s) 255, 268, 473
BfuAI ACCTGC 2 cut(s) 44, 262
BisI GCNGC 2 cut(s) 186, 412
BlsI GCNGC 2 cut(s) 187, 413
Bme1390I CCNGG 2 cut(s) 439, 491
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 2 cut(s) 439, 491
BmsI GCATC 2 cut(s) 127, 199
BpuEI CTTGAG 2 cut(s) 267, 509
BpuMI CCSGG 1 cut(s) 439
BsaJI CCNNGG 3 cut(s) 337, 438, 490
BsaWI WCCGGW 1 cut(s) 215
Bsc4I CCNNNNNNNGG 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 280
Bse3DI GCAATG 1 cut(s) 510
BseBI CCWGG 1 cut(s) 491
BseDI CCNNGG 3 cut(s) 337, 438, 490
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 1 cut(s) 343
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 1 cut(s) 280
BseXI GCAGC 2 cut(s) 172, 423
BsgI GTGCAG 2 cut(s) 171, 444
BshNI GGYRCC 1 cut(s) 46
BsiSI CCGG 2 cut(s) 216, 438
BslI CCNNNNNNNGG 1 cut(s) 343
BsmAI GTCTC 1 cut(s) 293
Bsp143I GATC 1 cut(s) 399
Bsp19I CCATGG 1 cut(s) 337
BspCNI CTCAG 1 cut(s) 337
BspLI GGNNCC 1 cut(s) 48
BspMAI CTGCAG 1 cut(s) 259
BspMI ACCTGC 2 cut(s) 44, 262
BspT107I GGYRCC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 510
BsrI ACTGG 1 cut(s) 280
BssECI CCNNGG 3 cut(s) 337, 438, 490
BssMI GATC 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 491
Bst4CI ACNGT 2 cut(s) 192, 240
BstC8I GCNNGC 4 cut(s) 247, 379, 480, 508
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 337
BstEII GGTNACC 1 cut(s) 31
BstF5I GGATG 1 cut(s) 448
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 1 cut(s) 402
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 1 cut(s) 399
BstMWI GCNNNNNNNGC 2 cut(s) 314, 512
BstNI CCWGG 1 cut(s) 491
BstNSI RCATGY 1 cut(s) 385
BstPI GGTNACC 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 437, 489
BstSFI CTRYAG 3 cut(s) 255, 268, 473
BstV1I GCAGC 2 cut(s) 172, 423
BtgI CCRYGG 1 cut(s) 337
BtsCI GGATG 1 cut(s) 448
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 2 cut(s) 56, 273
BveI ACCTGC 2 cut(s) 44, 262
Cac8I GCNNGC 4 cut(s) 247, 379, 480, 508
CfoI GCGC 1 cut(s) 91
Csp6I GTAC 3 cut(s) 47, 107, 364
CviAII CATG 3 cut(s) 43, 338, 382
CviJI RGCY 7 cut(s) 130, 185, 317, 328, 377, 414, 482
CviKI_1 RGCY 7 cut(s) 130, 185, 317, 328, 377, 414, 482
CviQI GTAC 3 cut(s) 47, 107, 364
DdeI CTNAG 1 cut(s) 324
DpnI GATC 1 cut(s) 401
DpnII GATC 1 cut(s) 399
Eco130I CCWWGG 1 cut(s) 337
Eco91I GGTNACC 1 cut(s) 31
EcoO65I GGTNACC 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 3 cut(s) 46, 341, 385
FaiI YATR 6 cut(s) 44, 226, 339, 383, 420, 435
FalI AAGNNNNNCTT 2 cut(s) 442, 474
FatI CATG 3 cut(s) 42, 337, 381
Fnu4HI GCNGC 2 cut(s) 186, 412
FokI GGATG 1 cut(s) 455
Fsp4HI GCNGC 2 cut(s) 186, 412
FspBI CTAG 1 cut(s) 168
GlaI GCGC 1 cut(s) 90
GluI GCNGC 2 cut(s) 186, 412
HapII CCGG 2 cut(s) 216, 438
HhaI GCGC 1 cut(s) 91
Hin1II CATG 3 cut(s) 46, 341, 385
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HindIII AAGCTT 1 cut(s) 480
HpaII CCGG 2 cut(s) 216, 438
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 4 cut(s) 149, 293, 334, 366
Hpy188I TCNGA 2 cut(s) 136, 463
Hpy8I GTNNAC 4 cut(s) 149, 293, 334, 366
HpyAV CCTTC 1 cut(s) 468
HpyCH4III ACNGT 2 cut(s) 192, 240
HpyCH4V TGCA 6 cut(s) 61, 119, 188, 212, 257, 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 512
HpyF3I CTNAG 1 cut(s) 324
Hsp92II CATG 3 cut(s) 46, 341, 385
HspAI GCGC 1 cut(s) 89
KpnI GGTACC 1 cut(s) 50
Kzo9I GATC 1 cut(s) 399
LmnI GCTCC 2 cut(s) 374, 394
Lsp1109I GCAGC 2 cut(s) 172, 423
LweI GCATC 2 cut(s) 127, 199
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 1 cut(s) 31
MalI GATC 1 cut(s) 401
MboI GATC 1 cut(s) 399
MfeI CAATTG 1 cut(s) 357
MluCI AATT 3 cut(s) 17, 261, 357
MnlI CCTC 4 cut(s) 61, 212, 222, 281
MspI CCGG 2 cut(s) 216, 438
MspR9I CCNGG 2 cut(s) 439, 491
MunI CAATTG 1 cut(s) 357
MvaI CCWGG 1 cut(s) 491
MwoI GCNNNNNNNGC 2 cut(s) 314, 512
NciI CCSGG 1 cut(s) 439
NcoI CCATGG 1 cut(s) 337
NdeII GATC 1 cut(s) 399
NlaIII CATG 3 cut(s) 46, 341, 385
NlaIV GGNNCC 1 cut(s) 48
NspI RCATGY 1 cut(s) 385
PflMI CCANNNNNTGG 1 cut(s) 343
PkrI GCNGC 2 cut(s) 187, 413
Psp6I CCWGG 1 cut(s) 489
PspEI GGTNACC 1 cut(s) 31
PspGI CCWGG 1 cut(s) 489
PspN4I GGNNCC 1 cut(s) 48
PsrI GAACNNNNNNTAC 2 cut(s) 456, 488
PstI CTGCAG 1 cut(s) 259
RsaI GTAC 3 cut(s) 48, 108, 365
RsaNI GTAC 3 cut(s) 47, 107, 364
SatI GCNGC 2 cut(s) 186, 412
Sau3AI GATC 1 cut(s) 399
SbfI CCTGCAGG 1 cut(s) 259
ScrFI CCNGG 2 cut(s) 439, 491
SdaI CCTGCAGG 1 cut(s) 259
SfaNI GCATC 2 cut(s) 127, 199
SfcI CTRYAG 3 cut(s) 255, 268, 473
SmlI CTYRAG 2 cut(s) 282, 524
SmoI CTYRAG 2 cut(s) 282, 524
Sse8387I CCTGCAGG 1 cut(s) 259
Sse9I AATT 3 cut(s) 17, 261, 357
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 437, 489
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 2 cut(s) 192, 240
TaqI TCGA 1 cut(s) 468
TasI AATT 3 cut(s) 17, 261, 357
TscAI CASTG 2 cut(s) 63, 280
TseI GCWGC 2 cut(s) 185, 411
TspDTI ATGAA 1 cut(s) 31
TspRI CASTG 2 cut(s) 63, 280
Van91I CCANNNNNTGG 1 cut(s) 343
XapI RAATTY 2 cut(s) 17, 261
XceI RCATGY 1 cut(s) 385
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.