Rorug04G0414500

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0013815
Physical Location & Seq
Reverse (-)
57599505 .. 57600815
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0414500.1

Sequence Viewer

Length: 792 bp
ATGATCGATTTCATATGTTATGCAGTGGTTCCAGTGACGTTGCCTGAGCAAAGCAGAAGTAGCAGTGGTTTTTATAGTGATAAACAACCGGCAGCTGCAGCTCCTTCGCGAAGTTCGCGATGGAATCCAACCCCGGAGCAGCTAATGGCCCTGGAAGAGCTGTATCGCTCTGGGATCAAGACACCGACGGCCCAACAAATCCGACAGGTGACTGCGCGGCTTCGAAACTATGGGAGGATTGAAGGCAAGAATGTATTCTACTGGTTTCAGAACCACCGAGCCAGGGAGAGGCAGAAGAGACGCCGTGAACTCATGTCCAAGTATCAGTCTGACTCTAAACAACAACAACAACAACAAACTTTGACTAACACCACAATTAGCAGTCTCCCGGACAAAGAATCTGCAGGAGTTGACTTTGTTGGTCGTCTTGAAGTTGAGCAGCAGAATAAGCGGACACTACCAAAGTTCACTTTACTTTCTGATCAGGAATCATCTTCAGTACCGCAAGAGAGGATGATGAGCATGGTGAAAAGAAATTCCTCCAGGCAACTTGTGGAACAATCCGGTTCACCTGGACCGACCTCAGACACAAATGCGTCAGCTACATTAATTAACATAAAGCTCTTGGACTTTCATCACTATTACGATTACGATTACGGTATGATATTGACTACACCCAATATTTACAAAGACGAGCAAGAAGAAGAAAGTAGAGAAATTCAAACTCTGGAGCTCTTTCCACTTGAGAGCGATAATCTCAAGGATGCTAAGGATACGAAATTAAACCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

263

Amino Acids

30.34

Weight (kDa)

7.77

Isoelectric Point (pI)

83.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 37 - 98 1.9e-14 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013815)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54500
fragaria_vesca FvH4_3g03210 FvH4_3g03210
rosa_chinensis RchiOBHm_Chr5g0004841
rosa_laevigata RLG00000031266
rosa_multiflora Rmu_sc0010871.1_g000002
rosa_roxburghii Rroxscaffold_1G00070770
rosa_rugosa Rorug04G0414400 Rorug04G0414400 Rorug04G0414500 Rorug04G0414600 Rorug04G0414700.1
rosa_samantha Rh5AG042200 Rh5BG041900 Rh5CG045700 Rh5DG042300
rosa_wichuraiana Rw5G004080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 109, 118, 217
AciI CCGC 3 cut(s) 217, 451, 503
AclWI GGATC 1 cut(s) 182
AcsI RAATTY 2 cut(s) 535, 717
AcuI CTGAAG 1 cut(s) 480
AcyI GRCGYC 1 cut(s) 301
AfaI GTAC 1 cut(s) 501
AfiI CCNNNNNNNGG 2 cut(s) 283, 288
AgsI TTSAA 3 cut(s) 242, 431, 722
AjnI CCWGG 4 cut(s) 150, 281, 542, 571
AluBI AGCT 7 cut(s) 95, 101, 142, 160, 602, 622, 733
AluI AGCT 7 cut(s) 95, 101, 142, 160, 602, 622, 733
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 2 cut(s) 292, 389
AlwI GGATC 1 cut(s) 182
AoxI GGCC 2 cut(s) 147, 189
ApeKI GCWGC 5 cut(s) 92, 95, 98, 139, 439
ApoI RAATTY 2 cut(s) 535, 717
AseI ATTAAT 1 cut(s) 608
Asp700I GAANNNNTTC 1 cut(s) 254
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 3 cut(s) 148, 190, 575
AsuC2I CCSGG 2 cut(s) 134, 389
AsuHPI GGTGA 3 cut(s) 220, 538, 561
AsuII TTCGAA 1 cut(s) 223
AvaII GGWCC 1 cut(s) 575
BanII GRGCYC 1 cut(s) 735
Bbv12I GWGCWC 1 cut(s) 735
BbvI GCAGC 5 cut(s) 82, 104, 110, 151, 451
BccI CCATC 1 cut(s) 114
BceAI ACGGC 2 cut(s) 204, 288
BcgI CGANNNNNNTGC 2 cut(s) 87, 121
BciT130I CCWGG 4 cut(s) 152, 283, 544, 573
BciVI GTATCC 1 cut(s) 766
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 2 cut(s) 134, 389
BcoDI GTCTC 2 cut(s) 292, 389
BfmI CTRYAG 2 cut(s) 96, 402
BfuI GTATCC 1 cut(s) 766
BisI GCNGC 6 cut(s) 93, 96, 99, 140, 218, 440
BlsI GCNGC 6 cut(s) 94, 97, 100, 141, 219, 441
Bme1390I CCNGG 6 cut(s) 134, 152, 283, 389, 544, 573
Bme18I GGWCC 1 cut(s) 575
BmgT120I GGNCC 3 cut(s) 148, 190, 575
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 6 cut(s) 134, 152, 283, 389, 544, 573
BmsI GCATC 1 cut(s) 754
BpmI CTGGAG 2 cut(s) 526, 749
Bpu10I CCTNAGC 2 cut(s) 45, 768
Bpu14I TTCGAA 1 cut(s) 223
BpuEI CTTGAG 2 cut(s) 743, 764
BpuMI CCSGG 2 cut(s) 134, 389
Bsa29I ATCGAT 1 cut(s) 6
BsaHI GRCGYC 1 cut(s) 301
BsaJI CCNNGG 3 cut(s) 132, 150, 282
BsaWI WCCGGW 1 cut(s) 563
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 288
Bse118I RCCGGY 1 cut(s) 88
Bse1I ACTGG 2 cut(s) 32, 266
BseBI CCWGG 4 cut(s) 152, 283, 544, 573
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 3 cut(s) 132, 150, 282
BseGI GGATG 2 cut(s) 519, 769
BseLI CCNNNNNNNGG 2 cut(s) 283, 288
BseMII CTCAG 2 cut(s) 36, 597
BseNI ACTGG 2 cut(s) 32, 266
BseXI GCAGC 5 cut(s) 82, 104, 110, 151, 451
Bsh1236I CGCG 3 cut(s) 109, 118, 217
BshFI GGCC 2 cut(s) 149, 191
BshVI ATCGAT 1 cut(s) 6
BsiHKAI GWGCWC 1 cut(s) 735
BsiSI CCGG 4 cut(s) 89, 134, 389, 564
BslI CCNNNNNNNGG 2 cut(s) 283, 288
BsmAI GTCTC 2 cut(s) 292, 389
BsmBI CGTCTC 1 cut(s) 292
BsnI GGCC 2 cut(s) 149, 191
Bsp119I TTCGAA 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 735
Bsp143I GATC 3 cut(s) 3, 174, 481
Bsp68I TCGCGA 2 cut(s) 109, 118
BspACI CCGC 3 cut(s) 217, 451, 503
BspANI GGCC 2 cut(s) 149, 191
BspCNI CTCAG 2 cut(s) 37, 596
BspDI ATCGAT 1 cut(s) 6
BspFNI CGCG 3 cut(s) 109, 118, 217
BspLI GGNNCC 1 cut(s) 30
BspMAI CTGCAG 2 cut(s) 100, 406
BspPI GGATC 1 cut(s) 182
BspQI GCTCTTC 1 cut(s) 150
BspT104I TTCGAA 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 88
BsrI ACTGG 2 cut(s) 32, 266
BssAI RCCGGY 1 cut(s) 88
BssECI CCNNGG 3 cut(s) 132, 150, 282
BssMI GATC 3 cut(s) 3, 174, 481
BssNI GRCGYC 1 cut(s) 301
Bst2UI CCWGG 4 cut(s) 152, 283, 544, 573
Bst4CI ACNGT 1 cut(s) 659
Bst6I CTCTTC 2 cut(s) 150, 290
BstACI GRCGYC 1 cut(s) 301
BstBI TTCGAA 1 cut(s) 223
BstDEI CTNAG 3 cut(s) 45, 583, 768
BstF5I GGATG 2 cut(s) 519, 769
BstFNI CGCG 3 cut(s) 109, 118, 217
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 3 cut(s) 6, 177, 484
BstMAI GTCTC 2 cut(s) 292, 389
BstMBI GATC 3 cut(s) 3, 174, 481
BstMWI GCNNNNNNNGC 4 cut(s) 60, 98, 115, 448
BstNI CCWGG 4 cut(s) 152, 283, 544, 573
BstSCI CCNGG 6 cut(s) 132, 150, 281, 387, 542, 571
BstSFI CTRYAG 2 cut(s) 96, 402
BstUI CGCG 3 cut(s) 109, 118, 217
BstV1I GCAGC 5 cut(s) 82, 104, 110, 151, 451
Bsu15I ATCGAT 1 cut(s) 6
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 2 cut(s) 149, 191
BsuTUI ATCGAT 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 133
BtsCI GGATG 2 cut(s) 519, 769
BtsI GCAGTG 2 cut(s) 30, 70
BtsIMutI CAGTG 3 cut(s) 30, 39, 70
BtuMI TCGCGA 2 cut(s) 109, 118
CfoI GCGC 1 cut(s) 217
Cfr10I RCCGGY 1 cut(s) 88
Cfr13I GGNCC 3 cut(s) 148, 190, 575
ClaI ATCGAT 1 cut(s) 6
CseI GACGC 2 cut(s) 309, 585
Csp6I GTAC 1 cut(s) 500
CviAII CATG 2 cut(s) 313, 523
CviQI GTAC 1 cut(s) 500
DdeI CTNAG 3 cut(s) 45, 583, 768
DpnI GATC 3 cut(s) 5, 176, 483
DpnII GATC 3 cut(s) 3, 174, 481
Eam1104I CTCTTC 2 cut(s) 150, 290
EarI CTCTTC 2 cut(s) 150, 290
Ecl136II GAGCTC 1 cut(s) 733
Eco24I GRGCYC 1 cut(s) 735
Eco47I GGWCC 1 cut(s) 575
Eco53kI GAGCTC 1 cut(s) 733
Eco57I CTGAAG 1 cut(s) 480
EcoICRI GAGCTC 1 cut(s) 733
EcoRII CCWGG 4 cut(s) 150, 281, 542, 571
EcoT38I GRGCYC 1 cut(s) 735
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 2 cut(s) 316, 526
FatI CATG 2 cut(s) 312, 522
FauNDI CATATG 1 cut(s) 14
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 6 cut(s) 93, 96, 99, 140, 218, 440
FokI GGATG 2 cut(s) 526, 776
FriOI GRGCYC 1 cut(s) 735
Fsp4HI GCNGC 6 cut(s) 93, 96, 99, 140, 218, 440
GlaI GCGC 1 cut(s) 216
GluI GCNGC 6 cut(s) 93, 96, 99, 140, 218, 440
GsuI CTGGAG 2 cut(s) 526, 749
HaeIII GGCC 2 cut(s) 149, 191
HapII CCGG 4 cut(s) 89, 134, 389, 564
HgaI GACGC 2 cut(s) 309, 585
HhaI GCGC 1 cut(s) 217
Hin1I GRCGYC 1 cut(s) 301
Hin1II CATG 2 cut(s) 316, 526
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 4 cut(s) 124, 332, 398, 488
HpaII CCGG 4 cut(s) 89, 134, 389, 564
HphI GGTGA 3 cut(s) 220, 538, 561
Hpy166II GTNNAC 4 cut(s) 308, 412, 468, 569
Hpy188I TCNGA 5 cut(s) 203, 270, 331, 481, 586
Hpy188III TCNNGA 6 cut(s) 108, 117, 178, 428, 485, 728
Hpy8I GTNNAC 4 cut(s) 308, 412, 468, 569
Hpy99I CGWCG 1 cut(s) 190
HpyAV CCTTC 2 cut(s) 114, 236
HpyCH4III ACNGT 1 cut(s) 659
HpyCH4IV ACGT 1 cut(s) 38
HpyCH4V TGCA 3 cut(s) 23, 98, 404
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 98, 115, 448
HpyF3I CTNAG 3 cut(s) 45, 583, 768
HpySE526I ACGT 1 cut(s) 38
Hsp92I GRCGYC 1 cut(s) 301
Hsp92II CATG 2 cut(s) 316, 526
HspAI GCGC 1 cut(s) 215
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 3 cut(s) 3, 174, 481
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 3 cut(s) 106, 136, 730
Lsp1109I GCAGC 5 cut(s) 82, 104, 110, 151, 451
LweI GCATC 1 cut(s) 754
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 2 cut(s) 34, 208
MalI GATC 3 cut(s) 5, 176, 483
MboI GATC 3 cut(s) 3, 174, 481
MboII GAAGA 5 cut(s) 167, 307, 486, 713, 716
MhlI GDGCHC 1 cut(s) 735
MluCI AATT 5 cut(s) 375, 535, 609, 717, 779
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 2 cut(s) 152, 226
MnlI CCTC 5 cut(s) 228, 282, 504, 550, 592
MroXI GAANNNNTTC 1 cut(s) 254
MseI TTAA 3 cut(s) 608, 612, 782
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 4 cut(s) 89, 134, 389, 564
MspR9I CCNGG 6 cut(s) 134, 152, 283, 389, 544, 573
MvaI CCWGG 4 cut(s) 152, 283, 544, 573
MvnI CGCG 3 cut(s) 109, 118, 217
MwoI GCNNNNNNNGC 4 cut(s) 60, 98, 115, 448
NciI CCSGG 2 cut(s) 134, 389
NdeI CATATG 1 cut(s) 14
NdeII GATC 3 cut(s) 3, 174, 481
NlaIII CATG 2 cut(s) 316, 526
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 34, 208
NruI TCGCGA 2 cut(s) 109, 118
NspV TTCGAA 1 cut(s) 223
PacI TTAATTAA 1 cut(s) 612
PciSI GCTCTTC 1 cut(s) 150
PdmI GAANNNNTTC 1 cut(s) 254
PfeI GAWTC 3 cut(s) 124, 398, 488
PfoI TCCNGGA 1 cut(s) 387
PkrI GCNGC 6 cut(s) 94, 97, 100, 141, 219, 441
PleI GAGTC 1 cut(s) 326
PpsI GAGTC 1 cut(s) 326
PshBI ATTAAT 1 cut(s) 608
Psp124BI GAGCTC 1 cut(s) 735
Psp6I CCWGG 4 cut(s) 150, 281, 542, 571
PspGI CCWGG 4 cut(s) 150, 281, 542, 571
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 3 cut(s) 148, 190, 575
PstI CTGCAG 2 cut(s) 100, 406
PvuII CAGCTG 1 cut(s) 95
RruI TCGCGA 2 cut(s) 109, 118
RsaI GTAC 1 cut(s) 501
RsaNI GTAC 1 cut(s) 500
SacI GAGCTC 1 cut(s) 735
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 3 cut(s) 608, 612, 782
SatI GCNGC 6 cut(s) 93, 96, 99, 140, 218, 440
Sau3AI GATC 3 cut(s) 3, 174, 481
Sau96I GGNCC 3 cut(s) 148, 190, 575
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 6 cut(s) 134, 152, 283, 389, 544, 573
SduI GDGCHC 1 cut(s) 735
SfaNI GCATC 1 cut(s) 754
SfcI CTRYAG 2 cut(s) 96, 402
SfuI TTCGAA 1 cut(s) 223
SinI GGWCC 1 cut(s) 575
SmlI CTYRAG 2 cut(s) 743, 758
SmoI CTYRAG 2 cut(s) 743, 758
Sse9I AATT 5 cut(s) 375, 535, 609, 717, 779
SsiI CCGC 3 cut(s) 217, 451, 503
SspI AATATT 1 cut(s) 682
SstI GAGCTC 1 cut(s) 735
StyD4I CCNGG 6 cut(s) 132, 150, 281, 387, 542, 571
TaaI ACNGT 1 cut(s) 659
TaiI ACGT 1 cut(s) 41
TaqI TCGA 2 cut(s) 6, 223
TaqII GACCGA 1 cut(s) 592
TasI AATT 5 cut(s) 375, 535, 609, 717, 779
TauI GCSGC 1 cut(s) 220
TfiI GAWTC 3 cut(s) 124, 398, 488
Tru1I TTAA 3 cut(s) 608, 612, 782
Tru9I TTAA 3 cut(s) 608, 612, 782
TscAI CASTG 3 cut(s) 30, 39, 70
TseFI GTSAC 2 cut(s) 34, 208
TseI GCWGC 5 cut(s) 92, 95, 98, 139, 439
Tsp45I GTSAC 2 cut(s) 34, 208
TspDTI ATGAA 1 cut(s) 623
TspRI CASTG 3 cut(s) 30, 39, 70
VpaK11BI GGWCC 1 cut(s) 575
VspI ATTAAT 1 cut(s) 608
XapI RAATTY 2 cut(s) 535, 717
XcmI CCANNNNNNNNNTGG 1 cut(s) 550
XmnI GAANNNNTTC 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.