AT5G66820

negative regulation of immature T cell proliferation in thymus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012944
Physical Location & Seq
Forward (+)
26688943 .. 26691180
2238 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66820.1

Sequence Viewer

Length: 1560 bp
ATGTTCTTTTCAGACATGGAGCGAAAAACCCTAGCGATTACGCCTCCTTCCTTCTTCTTCCACCTTGGAGGAGGAGCATTTGGTTCATCAAAGGCGTTTCATTGTCTTCTCCTTCTCTCATGTCTATTGCTTTCATCAGTCAATACATTGCATAACTTAGCTGAATATGGTTACAACACTGGTTCTGCTGCTATGGAGTTTCAGGGCTTTATTAATGGTGAAGATTCTGGGAGTGTAGCAATCCAGAAATCTGACCAGAATGTTTGTCCTAGTTCACAGCTGTTTTGTTTCCTTTCAGCTTTAGGATCAGAGAATCGGTCTGATCCATTTGTGTTGGCTTGTCCCAAAGAATCACGGAGTAGCTCTGCTCTGATCAGAAAAAATCTATCTGGTGTTGTGTGGCTATCTCTGAAGCCTGTCCACATCATAGAGTTTCAGCCATTTACTGGATTCTTTCACATTGGAGACACTTGTTACGAGCCTATGTCCAAAGAGCTCTATACTAAGAAGACCACAAGGGAGTTAAGTATTACGGTTTCAGGAAAACAATGTGGAGGAAATGGTTTTATGGTGAACCATCCATGTGAAGGATTTTCATTAGAACCAGGGGATTCCATAAAGTTTCTCTTTTTCTACCAGAGTGAACTCTCTTGGGCATCTGGAGTTGCTGTTTTTGCAGTACCTATGAAGGCGACCGCACCTGTTTTGATGCTTAGTCTCTACAAAAAACCGGTCTTTTGGGTGCGGACGAAGAAATTCGCAATTGCGGTTCTTATTGCTGCAGCTCTACTCATTCTGATATTTTGCTTCAATGATCACTTCATCGAGGAGAACAACAAGAGAAACAATAGCAACCACATGGAGTCGAGAGAAGTTGAGAAACCATCTACGATCACAATATCCCCTGAAATGGATTCTCTGCTAAGATCCATAAGCAAAGAAAGCCTGCAAGTGTTCGATGAGGTTCCCAAGAACAGCAGCAGCGTTAAGCCAGTTGCTTCTTCACACGAAGAAGAAGCATCAGAAGCTGTGAATCTCACTGTAAAGACAGCGAAAGACAAGAAGAGGCGCCGTAACAAGAAAAAGAAGAAAGGAGGAATCAACGGATTAACACCAGAGTGCACAGATGTTTCAAGCAGTTACAGCGGAAACTCAACTCCTAGGTCTCCAATATCACCAGAACCACCTACTACTCAAGCCGCAACGAAGCTAGTAAAGCCTCCTACTAAACCGGTCCTATCGCACTCAGCAACGTTTCCGGTTTCAGGGGTTAAGTCAATGATCATACAACGAAGCTCACTGGCTCCAAATGTGAGAGCACCTGGAGCGAAATCAAGAACCGAAGTGAAAGAAGAGAAAGCGAAAGAGTATAGGTACTATGATATCTGGGGAGATCACTTGACAGGGCTTAATTTGATGGATAAGTTAAAGGAAGTGAGAGAAGGTAAAAGCTCAGGTTTTGATGGAGAGGAGTGTGAGAGTTTCTTTGTTAAGGGTCCTCAGAACTTGTTAGCAGACTCTCACACCAGGTTTGTAAGTTTCTGTAACCAATGGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM131_like

Protein Analysis

519

Amino Acids

57.25

Weight (kDa)

8.82

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_TMEM131L_5 PF24501 176 - 217 6.2e-07 TMEM131L fifth Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66820
fragaria_vesca FvH4_1g15710 FvH4_1g15710
malus_domestica MD02G1169100.v1.1 MD15G1281500.v1.1
prunus_persica Prupe.7G136200_v2.0.a1
pyrus_communis pycom02g13580 pycom15g24460
rosa_chinensis RchiOBHm_Chr2g0105021
rosa_laevigata RLG00000017390
rosa_multiflora Rmu_sc0000641.1_g000023
rosa_roxburghii Rroxscaffold_2G00138570
rosa_rugosa Rorug02G0125200
rosa_samantha Rh2AG175500 Rh2BG183600 Rh2CG180200 Rh2DG181900
rosa_wichuraiana Rw2G013800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1068
AccB7I CCANNNNNTGG 1 cut(s) 446
AciI CCGC 5 cut(s) 696, 745, 767, 1146, 1200
AclI AACGTT 1 cut(s) 1253
AclWI GGATC 3 cut(s) 313, 317, 921
AcsI RAATTY 1 cut(s) 755
AcuI CTGAAG 1 cut(s) 431
AcyI GRCGYC 1 cut(s) 1069
AfaI GTAC 2 cut(s) 681, 1376
AfiI CCNNNNNNNGG 4 cut(s) 446, 587, 910, 1265
AgeI ACCGGT 2 cut(s) 730, 1231
AgsI TTSAA 2 cut(s) 811, 1134
AjnI CCWGG 3 cut(s) 604, 1321, 1526
AleI CACNNNNGTG 1 cut(s) 1117
Alw21I GWGCWC 3 cut(s) 498, 1124, 1321
Alw26I GTCTC 3 cut(s) 459, 722, 1170
Alw44I GTGCAC 1 cut(s) 1120
AlwI GGATC 3 cut(s) 313, 317, 921
AlwNI CAGNNNCTG 1 cut(s) 1028
ApaLI GTGCAC 1 cut(s) 1120
ApeKI GCWGC 5 cut(s) 188, 779, 782, 978, 981
ApoI RAATTY 1 cut(s) 755
ArsI GACNNNNNNTTYG 2 cut(s) 717, 749
AseI ATTAAT 1 cut(s) 213
AsiGI ACCGGT 2 cut(s) 730, 1231
Asp700I GAANNNNTTC 1 cut(s) 755
AspA2I CCTAGG 1 cut(s) 1160
AspLEI GCGC 1 cut(s) 1071
AspS9I GGNCC 2 cut(s) 1234, 1496
AsuHPI GGTGA 3 cut(s) 230, 583, 1167
AvaII GGWCC 2 cut(s) 1234, 1496
AvrII CCTAGG 1 cut(s) 1160
BaeGI GKGCMC 1 cut(s) 1124
BanI GGYRCC 1 cut(s) 1068
BanII GRGCYC 1 cut(s) 498
BbsI GAAGAC 2 cut(s) 98, 515
Bbv12I GWGCWC 3 cut(s) 498, 1124, 1321
BbvI GCAGC 5 cut(s) 175, 766, 794, 990, 993
BccI CCATC 4 cut(s) 585, 892, 1411, 1457
BceAI ACGGC 1 cut(s) 1056
BciT130I CCWGG 3 cut(s) 606, 1323, 1528
BclI TGATCA 3 cut(s) 372, 814, 1281
BcoDI GTCTC 3 cut(s) 459, 722, 1170
BfaI CTAG 4 cut(s) 32, 270, 1161, 1211
BfmI CTRYAG 1 cut(s) 780
BfoI RGCGCY 1 cut(s) 1072
BisI GCNGC 6 cut(s) 189, 780, 783, 979, 982, 1200
BlnI CCTAGG 1 cut(s) 1160
BlsI GCNGC 6 cut(s) 190, 781, 784, 980, 983, 1201
Bme1390I CCNGG 3 cut(s) 606, 1323, 1528
Bme18I GGWCC 2 cut(s) 1234, 1496
BmgT120I GGNCC 2 cut(s) 1234, 1496
BmiI GGNNCC 4 cut(s) 966, 1070, 1305, 1497
BmrFI CCNGG 3 cut(s) 606, 1323, 1528
BmsI GCATC 3 cut(s) 665, 699, 1028
BpiI GAAGAC 2 cut(s) 98, 515
BpmI CTGGAG 2 cut(s) 681, 1344
Bpu10I CCTNAGC 1 cut(s) 1453
BpuEI CTTGAG 1 cut(s) 1179
BsaHI GRCGYC 1 cut(s) 1069
BsaI GGTCTC 1 cut(s) 1170
BsaJI CCNNGG 3 cut(s) 64, 605, 1160
BsaWI WCCGGW 3 cut(s) 730, 1231, 1258
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 4 cut(s) 446, 587, 910, 1265
Bse118I RCCGGY 2 cut(s) 730, 1231
Bse1I ACTGG 4 cut(s) 184, 451, 992, 1305
Bse3DI GCAATG 1 cut(s) 146
BseBI CCWGG 3 cut(s) 606, 1323, 1528
BseDI CCNNGG 3 cut(s) 64, 605, 1160
BseGI GGATG 1 cut(s) 577
BseLI CCNNNNNNNGG 4 cut(s) 446, 587, 910, 1265
BseMI GCAATG 1 cut(s) 146
BseMII CTCAG 3 cut(s) 1260, 1467, 1514
BseNI ACTGG 4 cut(s) 184, 451, 992, 1305
BseRI GAGGAG 4 cut(s) 84, 87, 842, 1484
BseSI GKGCMC 1 cut(s) 1124
BseXI GCAGC 5 cut(s) 175, 766, 794, 990, 993
Bsh1285I CGRYCG 1 cut(s) 696
BshNI GGYRCC 1 cut(s) 1068
BshTI ACCGGT 2 cut(s) 730, 1231
BsiEI CGRYCG 1 cut(s) 696
BsiHKAI GWGCWC 3 cut(s) 498, 1124, 1321
BsiSI CCGG 3 cut(s) 731, 1232, 1259
BslFI GGGAC 1 cut(s) 327
BslI CCNNNNNNNGG 4 cut(s) 446, 587, 910, 1265
BsmAI GTCTC 3 cut(s) 459, 722, 1170
BsmFI GGGAC 1 cut(s) 327
Bso31I GGTCTC 1 cut(s) 1170
Bsp1286I GDGCHC 3 cut(s) 498, 1124, 1321
Bsp143I GATC 8 cut(s) 305, 322, 372, 814, 891, 926, 1281, 1393
BspACI CCGC 5 cut(s) 696, 745, 767, 1146, 1200
BspCNI CTCAG 3 cut(s) 1259, 1466, 1513
BspLI GGNNCC 4 cut(s) 966, 1070, 1305, 1497
BspMAI CTGCAG 1 cut(s) 784
BspPI GGATC 3 cut(s) 313, 317, 921
BspT107I GGYRCC 1 cut(s) 1068
BspTNI GGTCTC 1 cut(s) 1170
BsrDI GCAATG 1 cut(s) 146
BsrFI RCCGGY 2 cut(s) 730, 1231
BsrI ACTGG 4 cut(s) 184, 451, 992, 1305
BssAI RCCGGY 2 cut(s) 730, 1231
BssECI CCNNGG 3 cut(s) 64, 605, 1160
BssMI GATC 8 cut(s) 305, 322, 372, 814, 891, 926, 1281, 1393
BssNI GRCGYC 1 cut(s) 1069
BssT1I CCWWGG 2 cut(s) 64, 1160
Bst2UI CCWGG 3 cut(s) 606, 1323, 1528
Bst4CI ACNGT 2 cut(s) 535, 1042
Bst6I CTCTTC 2 cut(s) 1058, 1347
BstACI GRCGYC 1 cut(s) 1069
BstC8I GCNNGC 1 cut(s) 947
BstDEI CTNAG 7 cut(s) 157, 504, 713, 923, 1246, 1453, 1500
BstF5I GGATG 1 cut(s) 577
BstH2I RGCGCY 1 cut(s) 1072
BstHHI GCGC 1 cut(s) 1071
BstKTI GATC 8 cut(s) 308, 325, 375, 817, 894, 929, 1284, 1396
BstMAI GTCTC 3 cut(s) 459, 722, 1170
BstMBI GATC 8 cut(s) 305, 322, 372, 814, 891, 926, 1281, 1393
BstMCI CGRYCG 1 cut(s) 696
BstMWI GCNNNNNNNGC 6 cut(s) 674, 942, 1025, 1143, 1216, 1325
BstNI CCWGG 3 cut(s) 606, 1323, 1528
BstSCI CCNGG 3 cut(s) 604, 1321, 1526
BstSFI CTRYAG 1 cut(s) 780
BstSLI GKGCMC 1 cut(s) 1124
BstV1I GCAGC 5 cut(s) 175, 766, 794, 990, 993
BstV2I GAAGAC 2 cut(s) 98, 515
BstX2I RGATCY 1 cut(s) 926
BstYI RGATCY 1 cut(s) 926
BtsCI GGATG 1 cut(s) 577
BtsIMutI CAGTG 3 cut(s) 177, 1038, 1298
Cac8I GCNNGC 1 cut(s) 947
CaiI CAGNNNCTG 1 cut(s) 1028
CfoI GCGC 1 cut(s) 1071
Cfr10I RCCGGY 2 cut(s) 730, 1231
Cfr13I GGNCC 2 cut(s) 1234, 1496
CsiI ACCWGGT 1 cut(s) 1526
Csp6I GTAC 2 cut(s) 680, 1375
CspAI ACCGGT 2 cut(s) 730, 1231
CviAII CATG 4 cut(s) 16, 120, 582, 859
CviQI GTAC 2 cut(s) 680, 1375
DdeI CTNAG 7 cut(s) 157, 504, 713, 923, 1246, 1453, 1500
DinI GGCGCC 1 cut(s) 1070
DpnI GATC 8 cut(s) 307, 324, 374, 816, 893, 928, 1283, 1395
DpnII GATC 8 cut(s) 305, 322, 372, 814, 891, 926, 1281, 1393
Eam1104I CTCTTC 2 cut(s) 1058, 1347
EarI CTCTTC 2 cut(s) 1058, 1347
Ecl136II GAGCTC 1 cut(s) 496
Eco130I CCWWGG 2 cut(s) 64, 1160
Eco24I GRGCYC 1 cut(s) 498
Eco31I GGTCTC 1 cut(s) 1170
Eco32I GATATC 1 cut(s) 1384
Eco47I GGWCC 2 cut(s) 1234, 1496
Eco53kI GAGCTC 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 431
EcoICRI GAGCTC 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 1496
EcoRII CCWGG 3 cut(s) 604, 1321, 1526
EcoRV GATATC 1 cut(s) 1384
EcoT14I CCWWGG 2 cut(s) 64, 1160
EcoT38I GRGCYC 1 cut(s) 498
EgeI GGCGCC 1 cut(s) 1070
EheI GGCGCC 1 cut(s) 1070
ErhI CCWWGG 2 cut(s) 64, 1160
FaeI CATG 4 cut(s) 19, 123, 585, 862
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 1 cut(s) 327
FatI CATG 4 cut(s) 15, 119, 581, 858
FbaI TGATCA 3 cut(s) 372, 814, 1281
Fnu4HI GCNGC 6 cut(s) 189, 780, 783, 979, 982, 1200
FokI GGATG 1 cut(s) 564
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 6 cut(s) 189, 780, 783, 979, 982, 1200
FspBI CTAG 4 cut(s) 32, 270, 1161, 1211
GlaI GCGC 1 cut(s) 1070
GluI GCNGC 6 cut(s) 189, 780, 783, 979, 982, 1200
GsuI CTGGAG 2 cut(s) 681, 1344
HaeII RGCGCY 1 cut(s) 1072
HapII CCGG 3 cut(s) 731, 1232, 1259
HhaI GCGC 1 cut(s) 1071
Hin1I GRCGYC 1 cut(s) 1069
Hin1II CATG 4 cut(s) 19, 123, 585, 862
Hin6I GCGC 1 cut(s) 1069
HinP1I GCGC 1 cut(s) 1069
HpaII CCGG 3 cut(s) 731, 1232, 1259
HphI GGTGA 3 cut(s) 230, 583, 1167
Hpy166II GTNNAC 5 cut(s) 275, 421, 574, 644, 1122
Hpy188III TCNNGA 5 cut(s) 244, 540, 660, 867, 1335
Hpy8I GTNNAC 5 cut(s) 275, 421, 574, 644, 1122
HpyAV CCTTC 6 cut(s) 57, 61, 122, 581, 682, 1436
HpyCH4III ACNGT 2 cut(s) 535, 1042
HpyCH4IV ACGT 1 cut(s) 1253
HpyCH4V TGCA 5 cut(s) 151, 677, 782, 949, 1122
HpyF10VI GCNNNNNNNGC 6 cut(s) 674, 942, 1025, 1143, 1216, 1325
HpyF3I CTNAG 7 cut(s) 157, 504, 713, 923, 1246, 1453, 1500
HpySE526I ACGT 1 cut(s) 1253
Hsp92I GRCGYC 1 cut(s) 1069
Hsp92II CATG 4 cut(s) 19, 123, 585, 862
HspAI GCGC 1 cut(s) 1069
KasI GGCGCC 1 cut(s) 1068
Ksp22I TGATCA 3 cut(s) 372, 814, 1281
Kzo9I GATC 8 cut(s) 305, 322, 372, 814, 891, 926, 1281, 1393
LmnI GCTCC 4 cut(s) 19, 74, 1309, 1325
Lsp1109I GCAGC 5 cut(s) 175, 766, 794, 990, 993
LweI GCATC 3 cut(s) 665, 699, 1028
MabI ACCWGGT 1 cut(s) 1526
MaeI CTAG 4 cut(s) 32, 270, 1161, 1211
MaeII ACGT 1 cut(s) 1253
MaeIII GTNAC 5 cut(s) 170, 473, 1073, 1139, 1544
MalI GATC 8 cut(s) 307, 324, 374, 816, 893, 928, 1283, 1395
MboI GATC 8 cut(s) 305, 322, 372, 814, 891, 926, 1281, 1393
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 1 cut(s) 926
MhlI GDGCHC 3 cut(s) 498, 1124, 1321
MluCI AATT 3 cut(s) 755, 762, 1411
Mly113I GGCGCC 1 cut(s) 1069
MlyI GAGTC 2 cut(s) 872, 1511
MroXI GAANNNNTTC 1 cut(s) 755
MseI TTAA 8 cut(s) 213, 524, 987, 1109, 1272, 1410, 1427, 1491
MslI CAYNNNNRTG 2 cut(s) 582, 1117
MspA1I CMGCKG 2 cut(s) 280, 1146
MspI CCGG 3 cut(s) 731, 1232, 1259
MspR9I CCNGG 3 cut(s) 606, 1323, 1528
MunI CAATTG 1 cut(s) 762
MvaI CCWGG 3 cut(s) 606, 1323, 1528
MwoI GCNNNNNNNGC 6 cut(s) 674, 942, 1025, 1143, 1216, 1325
NarI GGCGCC 1 cut(s) 1069
NdeII GATC 8 cut(s) 305, 322, 372, 814, 891, 926, 1281, 1393
NlaIII CATG 4 cut(s) 19, 123, 585, 862
NlaIV GGNNCC 4 cut(s) 966, 1070, 1305, 1497
OliI CACNNNNGTG 1 cut(s) 1117
PdmI GAANNNNTTC 1 cut(s) 755
PfeI GAWTC 8 cut(s) 224, 313, 350, 450, 611, 914, 1033, 1098
PflMI CCANNNNNTGG 1 cut(s) 446
PinAI ACCGGT 2 cut(s) 730, 1231
PkrI GCNGC 6 cut(s) 190, 781, 784, 980, 983, 1201
PleI GAGTC 2 cut(s) 871, 1511
PluTI GGCGCC 1 cut(s) 1072
PpsI GAGTC 2 cut(s) 871, 1511
PpuMI RGGWCCY 1 cut(s) 1496
PshBI ATTAAT 1 cut(s) 213
Psp124BI GAGCTC 1 cut(s) 498
Psp1406I AACGTT 1 cut(s) 1253
Psp5II RGGWCCY 1 cut(s) 1496
Psp6I CCWGG 3 cut(s) 604, 1321, 1526
PspGI CCWGG 3 cut(s) 604, 1321, 1526
PspN4I GGNNCC 4 cut(s) 966, 1070, 1305, 1497
PspPI GGNCC 2 cut(s) 1234, 1496
PspPPI RGGWCCY 1 cut(s) 1496
PstI CTGCAG 1 cut(s) 784
PstNI CAGNNNCTG 1 cut(s) 1028
PsuI RGATCY 1 cut(s) 926
PvuII CAGCTG 1 cut(s) 280
RsaI GTAC 2 cut(s) 681, 1376
RsaNI GTAC 2 cut(s) 680, 1375
RseI CAYNNNNRTG 2 cut(s) 582, 1117
SacI GAGCTC 1 cut(s) 498
SaqAI TTAA 8 cut(s) 213, 524, 987, 1109, 1272, 1410, 1427, 1491
SatI GCNGC 6 cut(s) 189, 780, 783, 979, 982, 1200
Sau3AI GATC 8 cut(s) 305, 322, 372, 814, 891, 926, 1281, 1393
Sau96I GGNCC 2 cut(s) 1234, 1496
SchI GAGTC 2 cut(s) 872, 1511
ScrFI CCNGG 3 cut(s) 606, 1323, 1528
SduI GDGCHC 3 cut(s) 498, 1124, 1321
SexAI ACCWGGT 1 cut(s) 1526
SfaNI GCATC 3 cut(s) 665, 699, 1028
SfcI CTRYAG 1 cut(s) 780
SfoI GGCGCC 1 cut(s) 1070
SinI GGWCC 2 cut(s) 1234, 1496
SmiMI CAYNNNNRTG 2 cut(s) 582, 1117
SmlI CTYRAG 1 cut(s) 1194
SmoI CTYRAG 1 cut(s) 1194
Sse9I AATT 3 cut(s) 755, 762, 1411
SsiI CCGC 5 cut(s) 696, 745, 767, 1146, 1200
SspDI GGCGCC 1 cut(s) 1068
SspMI CTAG 4 cut(s) 32, 270, 1161, 1211
SstI GAGCTC 1 cut(s) 498
StyD4I CCNGG 3 cut(s) 604, 1321, 1526
StyI CCWWGG 2 cut(s) 64, 1160
TaaI ACNGT 2 cut(s) 535, 1042
TaiI ACGT 1 cut(s) 1256
TaqI TCGA 3 cut(s) 825, 866, 957
TaqII GACCGA 1 cut(s) 306
TasI AATT 3 cut(s) 755, 762, 1411
TauI GCSGC 1 cut(s) 1202
TfiI GAWTC 8 cut(s) 224, 313, 350, 450, 611, 914, 1033, 1098
Tru1I TTAA 8 cut(s) 213, 524, 987, 1109, 1272, 1410, 1427, 1491
Tru9I TTAA 8 cut(s) 213, 524, 987, 1109, 1272, 1410, 1427, 1491
TscAI CASTG 3 cut(s) 184, 1045, 1305
TseI GCWGC 5 cut(s) 188, 779, 782, 978, 981
TspDTI ATGAA 6 cut(s) 75, 89, 123, 585, 701, 811
TspGWI ACGGA 2 cut(s) 370, 1119
TspRI CASTG 3 cut(s) 184, 1045, 1305
Van91I CCANNNNNTGG 1 cut(s) 446
VneI GTGCAC 1 cut(s) 1120
VpaK11BI GGWCC 2 cut(s) 1234, 1496
VspI ATTAAT 1 cut(s) 213
XapI RAATTY 1 cut(s) 755
XmaJI CCTAGG 1 cut(s) 1160
XmnI GAANNNNTTC 1 cut(s) 755
XspI CTAG 4 cut(s) 32, 270, 1161, 1211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.