pycom02g13580

Transmembrane protein 131-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012944
Physical Location & Seq
Reverse (-)
10106909 .. 10108986
2078 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g13580.1

Sequence Viewer

Length: 1548 bp
ATGAGGCCCCTTAGGAACTGGGAGATTGGTCCACACAGCAGTGAAACTATCATAGAAATAGACTTCTCAATTGAATCGAAGGGAAAAATTTTTGGTGCAATTTGTATGCAGTTGCTAAGGCCTTCTGAGGACAAGTCTGATACTATTATGCTTCCTTTTGAGGCTGAAGTGGATGGAACAGCGACGAATGATGATCTTGCAGGACCTATCTCAGCATATCTTGAGGTTTTGGTCCCCTATTCTGCCAATGAGACTACTTCTGTTGCTATATCTCTGAAGAATTCTGCTCCTCACCTCTTGAGACTTCTTGAGATTACTGAAGTTGCGGATAGTAGAACTTTGCAGATTAAGTACATTGAAGGCTTGCTACTTTTCCCTGGCAGTGAAACATATGTTGCTGTTGTTGCTTGTACTGAGCCACATGTTGAATTAGATGGACACTGTAAATTACTTATACAGACAAATGACTCAAGTAGCTCGCAGATTGAAATTCCTTGCCAGGATGTTATTCATATATGTTCAAGACATTGGAATGATTCCACCATTGAATATGAACATCAGTCTGAAAGTAGTGAATCAGGTGATATGCAGACAGACTCCTCTGAAAGTGGCACACGGTGGCCATCACCAATCAAGGCAATGGAGGCAGCAGAAGCAGATGAACTGGTGCTACAAAACTGGAAATCTCAAGATACCAGAAGTGGCATGTCTGTGCTAACTGATCATGAGGTCTTCTTCCCAATGCTTCAGGTAGGAAGTCACAACTCTAAATGGATCACTGTAAAGAATCCTAGCCAAGAACCGGTAGTGATGCAGCTTATACTGAACTCAGGGGAGATTATTGATCGATGTAAGAGTCCAGATGGTCTTATACAGCCTCCTTCATCTGGTAGTGTGGTTTATAACGGATCCACTTCTCCAAGTAGGTATGGGTTCTCAGTAGCAGAAAATGCACTGACAGAGGCTTATGTTCAACCCAATGGTAGAGCATCTCTTGGACCAGTATTGTTTCACCCTTCAAATCGATGTGAATGGAGAAGTTCGGCCCTGATAAGAAACAATCTTTCTGGTGTGGAGTGGTTATCTCTGAGGGGATTTGGGGGGTCGCTATCCTTGCTTCTCCTTGAAGAGTCTGAGCCTGTTCAGAGTGTAGAATTCAACCTCAGTTTACAAATTCCTCTTAACTTTTCTCCTCCAGACATGTTTCGCATGGAAGATGTCACTCATTCTTGTTTACAGCCATTATCAAGGCAGCTCTATGCGAAGAATACAGGAGACTTGCCTCTGGAGGTTAGAAGAATCACAGTGTCTGGAAAAGAGTGCCGGATGGATGGGTTTATGGTACAAACTTGCGAAGGTTTTGCTCTTGAACCTGGAGAATCAACAAAACTTCTGATATCATACCACACTGATTTTTCTGCAGCCTTGGTACAACGGGATCTTGAGCTGGTCTTGAATTCTGGTATTCTGGTGATACCCATGAAGGCAAGCATCCCTTTGCATATCGATCAGTATATGCCAGAAATCAGTGGTCTGGATGCGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM131_like

Protein Analysis

516

Amino Acids

56.89

Weight (kDa)

4.72

Isoelectric Point (pI)

57.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7579 PF24474 69 - 177 2.3e-40 Domain of unknown function (DUF7579)
Ig_TMEM131L_5 PF24501 423 - 487 3.8e-24 TMEM131L fifth Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66820
fragaria_vesca FvH4_1g15710 FvH4_1g15710
malus_domestica MD02G1169100.v1.1 MD15G1281500.v1.1
prunus_persica Prupe.7G136200_v2.0.a1
pyrus_communis pycom02g13580 pycom15g24460
rosa_chinensis RchiOBHm_Chr2g0105021
rosa_laevigata RLG00000017390
rosa_multiflora Rmu_sc0000641.1_g000023
rosa_roxburghii Rroxscaffold_2G00138570
rosa_rugosa Rorug02G0125200
rosa_samantha Rh2AG175500 Rh2BG183600 Rh2CG180200 Rh2DG181900
rosa_wichuraiana Rw2G013800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 903
AccII CGCG 1 cut(s) 1543
AciI CCGC 1 cut(s) 326
AclWI GGATC 4 cut(s) 782, 903, 916, 1446
AcoI YGGCCR 1 cut(s) 620
AcsI RAATTY 6 cut(s) 87, 280, 489, 1154, 1173, 1456
AcuI CTGAAG 4 cut(s) 186, 296, 339, 731
AdeI CACNNNGTG 1 cut(s) 618
AfaI GTAC 4 cut(s) 353, 412, 1344, 1431
AfiI CCNNNNNNNGG 2 cut(s) 802, 887
AflIII ACRYGT 2 cut(s) 421, 1200
AgeI ACCGGT 1 cut(s) 802
AjnI CCWGG 3 cut(s) 376, 498, 1372
AleI CACNNNNGTG 1 cut(s) 39
AluBI AGCT 4 cut(s) 477, 817, 1255, 1447
AluI AGCT 4 cut(s) 477, 817, 1255, 1447
Alw26I GTCTC 3 cut(s) 245, 295, 1269
AlwI GGATC 4 cut(s) 782, 903, 916, 1446
AlwNI CAGNNNCTG 1 cut(s) 1310
AoxI GGCC 4 cut(s) 5, 119, 620, 1044
ApeKI GCWGC 4 cut(s) 647, 814, 1252, 1421
ApoI RAATTY 6 cut(s) 87, 280, 489, 1154, 1173, 1456
AsiGI ACCGGT 1 cut(s) 802
AspLEI GCGC 1 cut(s) 1543
AspS9I GGNCC 6 cut(s) 6, 29, 203, 232, 998, 1045
AsuHPI GGTGA 5 cut(s) 284, 593, 618, 1004, 1483
AvaII GGWCC 4 cut(s) 29, 203, 232, 998
AxyI CCTNAGG 1 cut(s) 11
BalI TGGCCA 1 cut(s) 622
BamHI GGATCC 1 cut(s) 908
BarI GAAGNNNNNNTAC 2 cut(s) 351, 383
BbsI GAAGAC 1 cut(s) 724
BbvI GCAGC 4 cut(s) 659, 826, 1264, 1433
BccI CCATC 6 cut(s) 167, 428, 631, 857, 1321, 1325
BcgI CGANNNNNNTGC 2 cut(s) 1343, 1377
BciT130I CCWGG 3 cut(s) 378, 500, 1374
BclI TGATCA 1 cut(s) 721
BcoDI GTCTC 3 cut(s) 245, 295, 1269
BfaI CTAG 1 cut(s) 792
BfmI CTRYAG 1 cut(s) 1419
BisI GCNGC 4 cut(s) 648, 815, 1253, 1422
BlsI GCNGC 4 cut(s) 649, 816, 1254, 1423
Bme1390I CCNGG 3 cut(s) 378, 500, 1374
Bme18I GGWCC 4 cut(s) 29, 203, 232, 998
BmgT120I GGNCC 6 cut(s) 6, 29, 203, 232, 998, 1045
BmiI GGNNCC 3 cut(s) 8, 234, 910
BmrFI CCNGG 3 cut(s) 378, 500, 1374
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 4 cut(s) 801, 998, 1500, 1528
BmuI ACTGGG 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 724
BpmI CTGGAG 3 cut(s) 1179, 1307, 1395
Bpu10I CCTNAGC 1 cut(s) 116
BpuEI CTTGAG 6 cut(s) 242, 319, 329, 454, 672, 1463
Bsa29I ATCGAT 3 cut(s) 847, 1024, 1506
BsaJI CCNNGG 2 cut(s) 376, 1425
BsaWI WCCGGW 1 cut(s) 802
Bsc4I CCNNNNNNNGG 2 cut(s) 802, 887
Bse118I RCCGGY 1 cut(s) 802
Bse1I ACTGG 4 cut(s) 23, 669, 683, 1001
Bse21I CCTNAGG 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 645
BseBI CCWGG 3 cut(s) 378, 500, 1374
BseCI ATCGAT 3 cut(s) 847, 1024, 1506
BseDI CCNNGG 2 cut(s) 376, 1425
BseGI GGATG 6 cut(s) 178, 508, 1332, 1336, 1491, 1543
BseLI CCNNNNNNNGG 2 cut(s) 802, 887
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 8 cut(s) 117, 225, 405, 843, 951, 1079, 1125, 1177
BseNI ACTGG 4 cut(s) 23, 669, 683, 1001
BseRI GAGGAG 3 cut(s) 279, 589, 1182
BseXI GCAGC 4 cut(s) 659, 826, 1264, 1433
Bsh1236I CGCG 1 cut(s) 1543
BshFI GGCC 4 cut(s) 7, 121, 622, 1046
BshTI ACCGGT 1 cut(s) 802
BshVI ATCGAT 3 cut(s) 847, 1024, 1506
BsiSI CCGG 2 cut(s) 803, 1324
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 2 cut(s) 802, 887
BsmAI GTCTC 3 cut(s) 245, 295, 1269
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 4 cut(s) 7, 121, 622, 1046
Bsp143I GATC 7 cut(s) 193, 721, 774, 844, 908, 1438, 1507
BspACI CCGC 1 cut(s) 326
BspANI GGCC 4 cut(s) 7, 121, 622, 1046
BspCNI CTCAG 8 cut(s) 118, 224, 406, 842, 950, 1080, 1126, 1176
BspDI ATCGAT 3 cut(s) 847, 1024, 1506
BspFNI CGCG 1 cut(s) 1543
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 3 cut(s) 8, 234, 910
BspMAI CTGCAG 1 cut(s) 1423
BspPI GGATC 4 cut(s) 782, 903, 916, 1446
BsrDI GCAATG 1 cut(s) 645
BsrFI RCCGGY 1 cut(s) 802
BsrI ACTGG 4 cut(s) 23, 669, 683, 1001
BssAI RCCGGY 1 cut(s) 802
BssECI CCNNGG 2 cut(s) 376, 1425
BssMI GATC 7 cut(s) 193, 721, 774, 844, 908, 1438, 1507
BssT1I CCWWGG 1 cut(s) 1425
Bst2UI CCWGG 3 cut(s) 378, 500, 1374
Bst4CI ACNGT 4 cut(s) 443, 618, 781, 1306
Bst6I CTCTTC 1 cut(s) 1122
BstAPI GCANNNNNTGC 1 cut(s) 950
BstC8I GCNNGC 3 cut(s) 365, 479, 1489
BstF5I GGATG 6 cut(s) 178, 508, 1332, 1336, 1491, 1543
BstFNI CGCG 1 cut(s) 1543
BstHHI GCGC 1 cut(s) 1543
BstKTI GATC 7 cut(s) 196, 724, 777, 847, 911, 1441, 1510
BstMAI GTCTC 3 cut(s) 245, 295, 1269
BstMBI GATC 7 cut(s) 193, 721, 774, 844, 908, 1438, 1507
BstMWI GCNNNNNNNGC 5 cut(s) 404, 644, 653, 950, 1114
BstNI CCWGG 3 cut(s) 378, 500, 1374
BstNSI RCATGY 3 cut(s) 425, 709, 1204
BstSCI CCNGG 3 cut(s) 376, 498, 1372
BstSFI CTRYAG 1 cut(s) 1419
BstUI CGCG 1 cut(s) 1543
BstV1I GCAGC 4 cut(s) 659, 826, 1264, 1433
BstV2I GAAGAC 1 cut(s) 724
BstX2I RGATCY 2 cut(s) 908, 1438
BstYI RGATCY 2 cut(s) 908, 1438
Bsu15I ATCGAT 3 cut(s) 847, 1024, 1506
Bsu36I CCTNAGG 1 cut(s) 11
BsuRI GGCC 4 cut(s) 7, 121, 622, 1046
BsuTUI ATCGAT 3 cut(s) 847, 1024, 1506
BtsCI GGATG 6 cut(s) 178, 508, 1332, 1336, 1491, 1543
BtsI GCAGTG 2 cut(s) 46, 388
BtsIMutI CAGTG 8 cut(s) 46, 388, 439, 777, 953, 1311, 1407, 1534
Cac8I GCNNGC 3 cut(s) 365, 479, 1489
CaiI CAGNNNCTG 1 cut(s) 1310
CciI TCATGA 1 cut(s) 724
CfoI GCGC 1 cut(s) 1543
Cfr10I RCCGGY 1 cut(s) 802
Cfr13I GGNCC 6 cut(s) 6, 29, 203, 232, 998, 1045
ClaI ATCGAT 3 cut(s) 847, 1024, 1506
Csp6I GTAC 4 cut(s) 352, 411, 1343, 1430
CspAI ACCGGT 1 cut(s) 802
CviAII CATG 6 cut(s) 422, 706, 725, 1201, 1210, 1480
CviQI GTAC 4 cut(s) 352, 411, 1343, 1430
DpnI GATC 7 cut(s) 195, 723, 776, 846, 910, 1440, 1509
DpnII GATC 7 cut(s) 193, 721, 774, 844, 908, 1438, 1507
DraIII CACNNNGTG 1 cut(s) 618
EaeI YGGCCR 1 cut(s) 620
Eam1104I CTCTTC 1 cut(s) 1122
EarI CTCTTC 1 cut(s) 1122
Eco130I CCWWGG 1 cut(s) 1425
Eco147I AGGCCT 1 cut(s) 121
Eco32I GATATC 1 cut(s) 1398
Eco47I GGWCC 4 cut(s) 29, 203, 232, 998
Eco57I CTGAAG 4 cut(s) 186, 296, 339, 731
Eco81I CCTNAGG 1 cut(s) 11
EcoO109I RGGNCCY 2 cut(s) 6, 203
EcoRI GAATTC 3 cut(s) 280, 1154, 1456
EcoRII CCWGG 3 cut(s) 376, 498, 1372
EcoRV GATATC 1 cut(s) 1398
EcoT14I CCWWGG 1 cut(s) 1425
ErhI CCWWGG 1 cut(s) 1425
FaeI CATG 6 cut(s) 425, 709, 728, 1204, 1213, 1483
FalI AAGNNNNNCTT 2 cut(s) 1480, 1512
FaqI GGGAC 1 cut(s) 218
FatI CATG 6 cut(s) 421, 705, 724, 1200, 1209, 1479
FauNDI CATATG 1 cut(s) 391
FbaI TGATCA 1 cut(s) 721
Fnu4HI GCNGC 4 cut(s) 648, 815, 1253, 1422
FokI GGATG 5 cut(s) 185, 515, 1339, 1343, 1478
Fsp4HI GCNGC 4 cut(s) 648, 815, 1253, 1422
FspBI CTAG 1 cut(s) 792
GlaI GCGC 1 cut(s) 1542
GluI GCNGC 4 cut(s) 648, 815, 1253, 1422
GsuI CTGGAG 3 cut(s) 1179, 1307, 1395
HaeIII GGCC 4 cut(s) 7, 121, 622, 1046
HapII CCGG 2 cut(s) 803, 1324
HhaI GCGC 1 cut(s) 1543
Hin1II CATG 6 cut(s) 425, 709, 728, 1204, 1213, 1483
Hin6I GCGC 1 cut(s) 1541
HinP1I GCGC 1 cut(s) 1541
HpaII CCGG 2 cut(s) 803, 1324
HphI GGTGA 5 cut(s) 284, 593, 618, 1004, 1483
Hpy166II GTNNAC 3 cut(s) 32, 1169, 1235
Hpy188I TCNGA 9 cut(s) 127, 139, 276, 565, 604, 1089, 1135, 1146, 1395
Hpy8I GTNNAC 3 cut(s) 32, 1169, 1235
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 7 cut(s) 73, 132, 353, 891, 1026, 1349, 1477
HpyCH4III ACNGT 4 cut(s) 443, 618, 781, 1306
HpyCH4V TGCA 9 cut(s) 98, 109, 200, 343, 589, 814, 953, 1421, 1501
HpyF10VI GCNNNNNNNGC 5 cut(s) 404, 644, 653, 950, 1114
Hsp92II CATG 6 cut(s) 425, 709, 728, 1204, 1213, 1483
HspAI GCGC 1 cut(s) 1541
Ksp22I TGATCA 1 cut(s) 721
Kzo9I GATC 7 cut(s) 193, 721, 774, 844, 908, 1438, 1507
LmnI GCTCC 1 cut(s) 292
Lsp1109I GCAGC 4 cut(s) 659, 826, 1264, 1433
LweI GCATC 4 cut(s) 801, 998, 1500, 1528
MaeI CTAG 1 cut(s) 792
MaeIII GTNAC 2 cut(s) 758, 1219
MalI GATC 7 cut(s) 195, 723, 776, 846, 910, 1440, 1509
MboI GATC 7 cut(s) 193, 721, 774, 844, 908, 1438, 1507
MboII GAAGA 7 cut(s) 289, 724, 727, 1139, 1226, 1276, 1308
MfeI CAATTG 1 cut(s) 69
MflI RGATCY 2 cut(s) 908, 1438
MlsI TGGCCA 1 cut(s) 622
MluNI TGGCCA 1 cut(s) 622
MlyI GAGTC 4 cut(s) 461, 590, 865, 1139
Mox20I TGGCCA 1 cut(s) 622
MscI TGGCCA 1 cut(s) 622
MseI TTAA 3 cut(s) 348, 1182, 1546
MslI CAYNNNNRTG 3 cut(s) 39, 531, 710
Msp20I TGGCCA 1 cut(s) 622
MspI CCGG 2 cut(s) 803, 1324
MspR9I CCNGG 3 cut(s) 378, 500, 1374
MunI CAATTG 1 cut(s) 69
MvaI CCWGG 3 cut(s) 378, 500, 1374
MvnI CGCG 1 cut(s) 1543
MwoI GCNNNNNNNGC 5 cut(s) 404, 644, 653, 950, 1114
NdeI CATATG 1 cut(s) 391
NdeII GATC 7 cut(s) 193, 721, 774, 844, 908, 1438, 1507
NlaIII CATG 6 cut(s) 425, 709, 728, 1204, 1213, 1483
NlaIV GGNNCC 3 cut(s) 8, 234, 910
NmuCI GTSAC 2 cut(s) 758, 1219
NspI RCATGY 3 cut(s) 425, 709, 1204
OliI CACNNNNGTG 1 cut(s) 39
PagI TCATGA 1 cut(s) 724
PceI AGGCCT 1 cut(s) 121
PciI ACATGT 2 cut(s) 421, 1200
PfeI GAWTC 6 cut(s) 74, 536, 575, 787, 1299, 1379
PinAI ACCGGT 1 cut(s) 802
PkrI GCNGC 4 cut(s) 649, 816, 1254, 1423
PleI GAGTC 4 cut(s) 461, 590, 864, 1138
PpsI GAGTC 4 cut(s) 461, 590, 864, 1138
PpuMI RGGWCCY 1 cut(s) 203
PscI ACATGT 2 cut(s) 421, 1200
PsiI TTATAA 1 cut(s) 903
Psp5II RGGWCCY 1 cut(s) 203
Psp6I CCWGG 3 cut(s) 376, 498, 1372
PspGI CCWGG 3 cut(s) 376, 498, 1372
PspN4I GGNNCC 3 cut(s) 8, 234, 910
PspPI GGNCC 6 cut(s) 6, 29, 203, 232, 998, 1045
PspPPI RGGWCCY 1 cut(s) 203
PsrI GAACNNNNNNTAC 2 cut(s) 654, 686
PstI CTGCAG 1 cut(s) 1423
PstNI CAGNNNCTG 1 cut(s) 1310
PsuI RGATCY 2 cut(s) 908, 1438
RsaI GTAC 4 cut(s) 353, 412, 1344, 1431
RsaNI GTAC 4 cut(s) 352, 411, 1343, 1430
RseI CAYNNNNRTG 3 cut(s) 39, 531, 710
SaqAI TTAA 3 cut(s) 348, 1182, 1546
SatI GCNGC 4 cut(s) 648, 815, 1253, 1422
Sau3AI GATC 7 cut(s) 193, 721, 774, 844, 908, 1438, 1507
Sau96I GGNCC 6 cut(s) 6, 29, 203, 232, 998, 1045
SchI GAGTC 4 cut(s) 461, 590, 865, 1139
ScrFI CCNGG 3 cut(s) 378, 500, 1374
SfaNI GCATC 4 cut(s) 801, 998, 1500, 1528
SfcI CTRYAG 1 cut(s) 1419
SinI GGWCC 4 cut(s) 29, 203, 232, 998
SmiMI CAYNNNNRTG 3 cut(s) 39, 531, 710
SmlI CTYRAG 6 cut(s) 221, 298, 308, 469, 687, 1442
SmoI CTYRAG 6 cut(s) 221, 298, 308, 469, 687, 1442
SseBI AGGCCT 1 cut(s) 121
SsiI CCGC 1 cut(s) 326
SspMI CTAG 1 cut(s) 792
StuI AGGCCT 1 cut(s) 121
StyD4I CCNGG 3 cut(s) 376, 498, 1372
StyI CCWWGG 1 cut(s) 1425
TaaI ACNGT 4 cut(s) 443, 618, 781, 1306
TaqI TCGA 4 cut(s) 77, 847, 1024, 1506
TatI WGTACW 2 cut(s) 351, 410
TfiI GAWTC 6 cut(s) 74, 536, 575, 787, 1299, 1379
Tru1I TTAA 3 cut(s) 348, 1182, 1546
Tru9I TTAA 3 cut(s) 348, 1182, 1546
TscAI CASTG 8 cut(s) 46, 388, 446, 784, 960, 1311, 1414, 1534
TseFI GTSAC 2 cut(s) 758, 1219
TseI GCWGC 4 cut(s) 647, 814, 1252, 1421
Tsp45I GTSAC 2 cut(s) 758, 1219
TspDTI ATGAA 5 cut(s) 500, 567, 675, 873, 1496
TspGWI ACGGA 1 cut(s) 921
TspRI CASTG 8 cut(s) 46, 388, 446, 784, 960, 1311, 1414, 1534
VpaK11BI GGWCC 4 cut(s) 29, 203, 232, 998
XapI RAATTY 6 cut(s) 87, 280, 489, 1154, 1173, 1456
XceI RCATGY 3 cut(s) 425, 709, 1204
XspI CTAG 1 cut(s) 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.