AT5G66920

Multicopper oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015973
Physical Location & Seq
Forward (+)
26722701 .. 26725657
2957 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66920.1

Sequence Viewer

Length: 1641 bp
ATGAAAATGGCATCAAGAAAAACAACATCCTTGCTTAATCATCTTCTTCTTCTTGGAGCTTTGACTCTTTTGAGCAGTTTGGTGATAGTGAAAGGAGAAAGCCCTTACAAGTTTTACACTTGGACTGTGACTTATGGCATTATCTCTCCTCTTGGTGTTCCTCAACAGGTTATTCTCATCAATGGTCAGTTTCCTGGTCCAAAACTTGAAGTTGTGACCAACGACAACATCATCCTCAACCTCATCAACAAACTTGACCAGCCTTTCCTCTTGACCTGGAATGGGATTAAGCAGAGGAAGAACTCATGGCAAGATGGTGTTTTGGGAACAAACTGTCCGATCCAACCAAACTCAAACTTCACTTACAAATTCCAAACCAAAGATCAGATCGGAACTTTCAACTACTTCCCTTCCACTGCTTTTCACAAAGCCGCCGGTGGATTCGGAGCCATCAATGTCTACGCAAGACCTGGAATTCCAATCCCTTACCCTCTCCCAACCGCAGATTTCACTTTACTCGTTGGTGATTGGTTCAAAACCAACCACAAAACGCTTCAACAGCGTTTGGATTCTGGTGGGGTTCTTCCATTTCCCGATGGGATGCTTATAAATGGACAAACCCAATCAACGTTTTCAGGCGACCAAGGGAAGACTTACATGTTGAGAATCTCCAATGTTGGGTTATCAAGCACTTTCAATTTCAGAATCCAAGGCCATACAATGAAAGTAGTTGAAGTTGAAGGCTCTCATGTTATTCAAACTGATTATGATTCTCTTGACATTCACGTTGGCCAGTCTTTAGCAGTTCTTGTTACTTTAAACCAATCTCCTAAAGATTATTACATCGTTGCAAGCACCCGGTTCATAAGAAGCAAGCTTTCTGTTATGGGTTTGTTGCGTTATTCTAACTCTCGCGTCCCAGCTTCTGGTGATCCTCCCGCTCTTCCACCTGGAGAGCTTGTTTGGTCCATGAGACAAGCTAGAACCTTCAGATGGAACTTAACAGCAAATGCAGCTAGACCAAACCCTCAAGGATCTTTCCACTATGGAATGATCAGTCCAACAAAGACATTTGTCTTCTCAAATTCAGCTCCTTTGATTAATGGGAAGCAAAGATATGCAGTCAATGGAGTCTCTTATGTGAAATCAGAGACGCCTCTTAAACTCGCTGATCACTTTGGCATCTCAGGAGTTTTCAGCACCAATGCCATTCAAAGCGTCCCATCAAACTCTCCGCCAACAGTTGCAACATCAGTTGTTCAAACATCTCACCATGACTTCCTTGAAATTGTATTTCAGAACAATGAGAAGTCAATGCAGTCTTGGCATCTTGATGGATATGACTTCTGGGTCGTTGGATTTGGTTCTGGACAATGGACACCTGCAAAAAGATCACTTCACAATCTTGTTGATGCTCTGACCAGACACACAACTCAGGTCTACCCAGAGTCTTGGACAACAATCTTAGTGTCTTTAGACAATCAAGGGATGTGGAATATGAGATCAGCGATATGGGAAAGACAATATTCAGGACAACAGTTTTATCTCAAAGTATGGAATTCAGTACAGAGTCTTGCCAATGAATACAATCCTCCTGACAATCTTCAACTCTGTGGCAAAGCTGTTGGACGACATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

546

Amino Acids

60.82

Weight (kDa)

9.48

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 43 - 156 4e-40 Multicopper oxidase
Cu-oxidase PF00394 169 - 303 3.3e-41 Multicopper oxidase
Cu-oxidase_2 PF07731 385 - 522 2e-31 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66920
fragaria_vesca FvH4_1g14990 FvH4_1g14990
malus_domestica MD15G1276700.v1.1
prunus_persica Prupe.7G140500_v2.0.a1
pyrus_communis pycom02g13010 pycom15g23960
rosa_chinensis RchiOBHm_Chr2g0104051
rosa_laevigata RLG00000017282
rosa_multiflora Rmu_ssc0000257.1_g000032
rosa_roxburghii Rroxscaffold_2G00139160
rosa_rugosa Rorug02G0119100
rosa_samantha Rh2CG174100 Rh2DG173700
rosa_wichuraiana Rw2G013160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 608
AarI CACCTGC 1 cut(s) 1390
AasI GACNNNNNNGTC 1 cut(s) 1349
Acc36I ACCTGC 1 cut(s) 1390
AccB7I CCANNNNNTGG 1 cut(s) 926
AccBSI CCGCTC 1 cut(s) 941
AccI GTMKAC 2 cut(s) 459, 1440
AccII CGCG 1 cut(s) 915
AciI CCGC 4 cut(s) 432, 501, 939, 1235
AclI AACGTT 1 cut(s) 629
AclWI GGATC 3 cut(s) 334, 926, 1042
AcoI YGGCCR 1 cut(s) 790
AcsI RAATTY 4 cut(s) 368, 474, 1084, 1558
AcuI CTGAAG 1 cut(s) 973
AcyI GRCGYC 1 cut(s) 1154
AfaI GTAC 1 cut(s) 1566
AfiI CCNNNNNNNGG 4 cut(s) 282, 678, 926, 993
AflIII ACRYGT 2 cut(s) 657, 1633
AjnI CCWGG 4 cut(s) 193, 275, 469, 949
AloI GAACNNNNNNTCC 2 cut(s) 319, 351
AluBI AGCT 8 cut(s) 59, 877, 923, 958, 980, 1016, 1091, 1622
AluI AGCT 8 cut(s) 59, 877, 923, 958, 980, 1016, 1091, 1622
Alw26I GTCTC 3 cut(s) 967, 1138, 1145
AlwI GGATC 3 cut(s) 334, 926, 1042
AlwNI CAGNNNCTG 1 cut(s) 926
AoxI GGCC 2 cut(s) 712, 790
ApeKI GCWGC 1 cut(s) 1013
ApoI RAATTY 4 cut(s) 368, 474, 1084, 1558
AseI ATTAAT 1 cut(s) 1101
AspS9I GGNCC 2 cut(s) 197, 966
AsuC2I CCSGG 1 cut(s) 859
AsuHPI GGTGA 4 cut(s) 94, 536, 941, 1262
AvaII GGWCC 2 cut(s) 197, 966
BalI TGGCCA 1 cut(s) 792
BbsI GAAGAC 2 cut(s) 656, 1069
BbvI GCAGC 1 cut(s) 1025
BccI CCATC 6 cut(s) 308, 458, 590, 987, 1231, 1328
BciT130I CCWGG 4 cut(s) 195, 277, 471, 951
BclI TGATCA 2 cut(s) 1053, 1171
BcnI CCSGG 1 cut(s) 859
BcoDI GTCTC 3 cut(s) 967, 1138, 1145
BfaI CTAG 2 cut(s) 981, 1017
BfuAI ACCTGC 1 cut(s) 1390
BisI GCNGC 2 cut(s) 432, 1014
BlsI GCNGC 2 cut(s) 433, 1015
Bme1390I CCNGG 5 cut(s) 195, 277, 471, 859, 951
Bme18I GGWCC 2 cut(s) 197, 966
BmgT120I GGNCC 2 cut(s) 197, 966
BmiI GGNNCC 1 cut(s) 448
BmrFI CCNGG 5 cut(s) 195, 277, 471, 859, 951
BmsI GCATC 5 cut(s) 20, 591, 1191, 1336, 1402
BoxI GACNNNNGTC 1 cut(s) 1073
BpiI GAAGAC 2 cut(s) 656, 1069
BpmI CTGGAG 1 cut(s) 972
BpuEI CTTGAG 1 cut(s) 1014
BpuMI CCSGG 1 cut(s) 859
BsaHI GRCGYC 1 cut(s) 1154
BsaJI CCNNGG 2 cut(s) 643, 709
BsaXI ACNNNNNCTCC 2 cut(s) 945, 975
Bsc4I CCNNNNNNNGG 4 cut(s) 282, 678, 926, 993
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 1 cut(s) 793
BseBI CCWGG 4 cut(s) 195, 277, 471, 951
BseDI CCNNGG 2 cut(s) 643, 709
BseGI GGATG 4 cut(s) 26, 231, 606, 1494
BseLI CCNNNNNNNGG 4 cut(s) 282, 678, 926, 993
BseMII CTCAG 2 cut(s) 1200, 1448
BseNI ACTGG 1 cut(s) 793
BseRI GAGGAG 1 cut(s) 138
BseXI GCAGC 1 cut(s) 1025
BseYI CCCAGC 1 cut(s) 919
Bsh1236I CGCG 1 cut(s) 915
BshFI GGCC 2 cut(s) 714, 792
BsiSI CCGG 2 cut(s) 435, 859
BslFI GGGAC 2 cut(s) 902, 1205
BslI CCNNNNNNNGG 4 cut(s) 282, 678, 926, 993
BsmAI GTCTC 3 cut(s) 967, 1138, 1145
BsmBI CGTCTC 1 cut(s) 1145
BsmFI GGGAC 2 cut(s) 902, 1205
BsnI GGCC 2 cut(s) 714, 792
Bsp143I GATC 9 cut(s) 339, 382, 387, 931, 1034, 1053, 1171, 1391, 1502
BspACI CCGC 4 cut(s) 432, 501, 939, 1235
BspANI GGCC 2 cut(s) 714, 792
BspCNI CTCAG 2 cut(s) 1199, 1447
BspFNI CGCG 1 cut(s) 915
BspLI GGNNCC 1 cut(s) 448
BspMI ACCTGC 1 cut(s) 1390
BspPI GGATC 3 cut(s) 334, 926, 1042
BspQI GCTCTTC 1 cut(s) 948
BsrBI CCGCTC 1 cut(s) 941
BsrFI RCCGGY 1 cut(s) 434
BsrI ACTGG 1 cut(s) 793
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 2 cut(s) 643, 709
BssMI GATC 9 cut(s) 339, 382, 387, 931, 1034, 1053, 1171, 1391, 1502
BssNI GRCGYC 1 cut(s) 1154
BssT1I CCWWGG 2 cut(s) 643, 709
Bst2UI CCWGG 4 cut(s) 195, 277, 471, 951
Bst4CI ACNGT 4 cut(s) 127, 335, 1243, 1539
Bst6I CTCTTC 1 cut(s) 948
BstACI GRCGYC 1 cut(s) 1154
BstC8I GCNNGC 2 cut(s) 853, 875
BstDEI CTNAG 3 cut(s) 1186, 1434, 1465
BstF5I GGATG 4 cut(s) 26, 231, 606, 1494
BstFNI CGCG 1 cut(s) 915
BstKTI GATC 9 cut(s) 342, 385, 390, 934, 1037, 1056, 1174, 1394, 1505
BstMAI GTCTC 3 cut(s) 967, 1138, 1145
BstMBI GATC 9 cut(s) 339, 382, 387, 931, 1034, 1053, 1171, 1391, 1502
BstMWI GCNNNNNNNGC 3 cut(s) 559, 1013, 1324
BstNI CCWGG 4 cut(s) 195, 277, 471, 951
BstNSI RCATGY 2 cut(s) 661, 1637
BstPAI GACNNNNGTC 1 cut(s) 1073
BstSCI CCNGG 5 cut(s) 193, 275, 469, 857, 949
BstUI CGCG 1 cut(s) 915
BstV1I GCAGC 1 cut(s) 1025
BstV2I GAAGAC 2 cut(s) 656, 1069
BstX2I RGATCY 1 cut(s) 1034
BstXI CCANNNNNNTGG 1 cut(s) 1452
BstYI RGATCY 1 cut(s) 1034
BsuRI GGCC 2 cut(s) 714, 792
BtsCI GGATG 4 cut(s) 26, 231, 606, 1494
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 1 cut(s) 414
BveI ACCTGC 1 cut(s) 1390
Cac8I GCNNGC 2 cut(s) 853, 875
CaiI CAGNNNCTG 1 cut(s) 926
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 197, 966
CseI GACGC 3 cut(s) 904, 1162, 1207
Csp6I GTAC 1 cut(s) 1565
CspCI CAANNNNNGTGG 2 cut(s) 1472, 1507
CviAII CATG 6 cut(s) 306, 658, 749, 970, 1274, 1634
CviQI GTAC 1 cut(s) 1565
DdeI CTNAG 3 cut(s) 1186, 1434, 1465
DpnI GATC 9 cut(s) 341, 384, 389, 933, 1036, 1055, 1173, 1393, 1504
DpnII GATC 9 cut(s) 339, 382, 387, 931, 1034, 1053, 1171, 1391, 1502
DraI TTTAAA 1 cut(s) 819
DrdI GACNNNNNNGTC 1 cut(s) 1349
DseDI GACNNNNNNGTC 1 cut(s) 1349
EaeI YGGCCR 1 cut(s) 790
Eam1104I CTCTTC 1 cut(s) 948
EarI CTCTTC 1 cut(s) 948
EciI GGCGGA 1 cut(s) 1224
Eco130I CCWWGG 2 cut(s) 643, 709
Eco47I GGWCC 2 cut(s) 197, 966
Eco57I CTGAAG 1 cut(s) 973
EcoRI GAATTC 2 cut(s) 474, 1558
EcoRII CCWGG 4 cut(s) 193, 275, 469, 949
EcoT14I CCWWGG 2 cut(s) 643, 709
ErhI CCWWGG 2 cut(s) 643, 709
Esp3I CGTCTC 1 cut(s) 1145
FaeI CATG 6 cut(s) 309, 661, 752, 973, 1277, 1637
FaqI GGGAC 2 cut(s) 902, 1205
FatI CATG 6 cut(s) 305, 657, 748, 969, 1273, 1633
FauI CCCGC 1 cut(s) 946
FbaI TGATCA 2 cut(s) 1053, 1171
FblI GTMKAC 2 cut(s) 459, 1440
Fnu4HI GCNGC 2 cut(s) 432, 1014
FokI GGATG 4 cut(s) 13, 218, 613, 1501
Fsp4HI GCNGC 2 cut(s) 432, 1014
FspBI CTAG 2 cut(s) 981, 1017
GluI GCNGC 2 cut(s) 432, 1014
GsaI CCCAGC 1 cut(s) 923
GsuI CTGGAG 1 cut(s) 972
HaeIII GGCC 2 cut(s) 714, 792
HapII CCGG 2 cut(s) 435, 859
HgaI GACGC 3 cut(s) 904, 1162, 1207
Hin1I GRCGYC 1 cut(s) 1154
Hin1II CATG 6 cut(s) 309, 661, 752, 973, 1277, 1637
HindIII AAGCTT 1 cut(s) 875
HinfI GANTC 9 cut(s) 64, 441, 569, 666, 705, 770, 1131, 1448, 1570
HpaII CCGG 2 cut(s) 435, 859
HphI GGTGA 4 cut(s) 94, 536, 941, 1262
Hpy166II GTNNAC 2 cut(s) 460, 1441
Hpy188I TCNGA 9 cut(s) 339, 387, 392, 446, 704, 992, 1150, 1299, 1419
Hpy188III TCNNGA 9 cut(s) 15, 271, 593, 776, 1188, 1331, 1368, 1530, 1595
Hpy8I GTNNAC 2 cut(s) 460, 1441
HpyAV CCTTC 3 cut(s) 420, 734, 997
HpyCH4III ACNGT 4 cut(s) 127, 335, 1243, 1539
HpyCH4IV ACGT 2 cut(s) 629, 786
HpyCH4V TGCA 6 cut(s) 851, 1013, 1121, 1247, 1318, 1385
HpyF10VI GCNNNNNNNGC 3 cut(s) 559, 1013, 1324
HpyF3I CTNAG 3 cut(s) 1186, 1434, 1465
HpySE526I ACGT 2 cut(s) 629, 786
Hsp92I GRCGYC 1 cut(s) 1154
Hsp92II CATG 6 cut(s) 309, 661, 752, 973, 1277, 1637
Ksp22I TGATCA 2 cut(s) 1053, 1171
Kzo9I GATC 9 cut(s) 339, 382, 387, 931, 1034, 1053, 1171, 1391, 1502
LguI GCTCTTC 1 cut(s) 948
LmnI GCTCC 3 cut(s) 56, 446, 1096
Lsp1109I GCAGC 1 cut(s) 1025
LweI GCATC 5 cut(s) 20, 591, 1191, 1336, 1402
MaeI CTAG 2 cut(s) 981, 1017
MaeII ACGT 2 cut(s) 629, 786
MaeIII GTNAC 3 cut(s) 127, 214, 811
MalI GATC 9 cut(s) 341, 384, 389, 933, 1036, 1055, 1173, 1393, 1504
MbiI CCGCTC 1 cut(s) 941
MboI GATC 9 cut(s) 339, 382, 387, 931, 1034, 1053, 1171, 1391, 1502
MboII GAAGA 9 cut(s) 35, 38, 41, 310, 575, 661, 935, 1069, 1595
MflI RGATCY 1 cut(s) 1034
MlsI TGGCCA 1 cut(s) 792
MluCI AATT 6 cut(s) 368, 474, 697, 1084, 1287, 1558
MluNI TGGCCA 1 cut(s) 792
MlyI GAGTC 4 cut(s) 58, 1140, 1457, 1579
MmeI TCCRAC 4 cut(s) 367, 1085, 1336, 1606
Mox20I TGGCCA 1 cut(s) 792
MscI TGGCCA 1 cut(s) 792
MseI TTAA 6 cut(s) 36, 288, 818, 1001, 1101, 1161
MslI CAYNNNNRTG 1 cut(s) 1332
Msp20I TGGCCA 1 cut(s) 792
MspI CCGG 2 cut(s) 435, 859
MspR9I CCNGG 5 cut(s) 195, 277, 471, 859, 951
MvaI CCWGG 4 cut(s) 195, 277, 471, 951
MvnI CGCG 1 cut(s) 915
MwoI GCNNNNNNNGC 3 cut(s) 559, 1013, 1324
NciI CCSGG 1 cut(s) 859
NdeII GATC 9 cut(s) 339, 382, 387, 931, 1034, 1053, 1171, 1391, 1502
NlaIII CATG 6 cut(s) 309, 661, 752, 973, 1277, 1637
NlaIV GGNNCC 1 cut(s) 448
NmuCI GTSAC 2 cut(s) 127, 214
NspI RCATGY 2 cut(s) 661, 1637
PaqCI CACCTGC 1 cut(s) 1390
PciI ACATGT 2 cut(s) 657, 1633
PciSI GCTCTTC 1 cut(s) 948
PfeI GAWTC 5 cut(s) 441, 569, 666, 705, 770
PflMI CCANNNNNTGG 1 cut(s) 926
PkrI GCNGC 2 cut(s) 433, 1015
PleI GAGTC 4 cut(s) 58, 1139, 1456, 1578
PpsI GAGTC 4 cut(s) 58, 1139, 1456, 1578
PscI ACATGT 2 cut(s) 657, 1633
PshAI GACNNNNGTC 1 cut(s) 1073
PshBI ATTAAT 1 cut(s) 1101
PsiI TTATAA 1 cut(s) 608
Psp1406I AACGTT 1 cut(s) 629
Psp6I CCWGG 4 cut(s) 193, 275, 469, 949
PspFI CCCAGC 1 cut(s) 919
PspGI CCWGG 4 cut(s) 193, 275, 469, 949
PspN4I GGNNCC 1 cut(s) 448
PspPI GGNCC 2 cut(s) 197, 966
PstNI CAGNNNCTG 1 cut(s) 926
PsuI RGATCY 1 cut(s) 1034
RsaI GTAC 1 cut(s) 1566
RsaNI GTAC 1 cut(s) 1565
RseI CAYNNNNRTG 1 cut(s) 1332
SapI GCTCTTC 1 cut(s) 948
SaqAI TTAA 6 cut(s) 36, 288, 818, 1001, 1101, 1161
SatI GCNGC 2 cut(s) 432, 1014
Sau3AI GATC 9 cut(s) 339, 382, 387, 931, 1034, 1053, 1171, 1391, 1502
Sau96I GGNCC 2 cut(s) 197, 966
SchI GAGTC 4 cut(s) 58, 1140, 1457, 1579
ScrFI CCNGG 5 cut(s) 195, 277, 471, 859, 951
SfaNI GCATC 5 cut(s) 20, 591, 1191, 1336, 1402
SgrAI CRCCGGYG 1 cut(s) 434
SinI GGWCC 2 cut(s) 197, 966
SmiMI CAYNNNNRTG 1 cut(s) 1332
SmlI CTYRAG 1 cut(s) 1029
SmoI CTYRAG 1 cut(s) 1029
Sse9I AATT 6 cut(s) 368, 474, 697, 1084, 1287, 1558
SsiI CCGC 4 cut(s) 432, 501, 939, 1235
SspI AATATT 1 cut(s) 1526
SspMI CTAG 2 cut(s) 981, 1017
StyD4I CCNGG 5 cut(s) 193, 275, 469, 857, 949
StyI CCWWGG 2 cut(s) 643, 709
TaaI ACNGT 4 cut(s) 127, 335, 1243, 1539
TaiI ACGT 2 cut(s) 632, 789
TasI AATT 6 cut(s) 368, 474, 697, 1084, 1287, 1558
TatI WGTACW 1 cut(s) 1564
TauI GCSGC 1 cut(s) 434
TfiI GAWTC 5 cut(s) 441, 569, 666, 705, 770
Tru1I TTAA 6 cut(s) 36, 288, 818, 1001, 1101, 1161
Tru9I TTAA 6 cut(s) 36, 288, 818, 1001, 1101, 1161
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 2 cut(s) 127, 214
TseI GCWGC 1 cut(s) 1013
Tsp45I GTSAC 2 cut(s) 127, 214
TspDTI ATGAA 4 cut(s) 17, 737, 853, 1596
TspRI CASTG 1 cut(s) 421
Van91I CCANNNNNTGG 1 cut(s) 926
VpaK11BI GGWCC 2 cut(s) 197, 966
VspI ATTAAT 1 cut(s) 1101
XapI RAATTY 4 cut(s) 368, 474, 1084, 1558
XceI RCATGY 2 cut(s) 661, 1637
XmiI GTMKAC 2 cut(s) 459, 1440
XspI CTAG 2 cut(s) 981, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.