Rorug02G0119100

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015973
Physical Location & Seq
Reverse (-)
10196054 .. 10197249
1196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0119100.1

Sequence Viewer

Length: 804 bp
ATGTTGAAGGAAGTGAGTGAAGAAGGTTTAGAGGTTCACGAAGAGCAGCAGCGTGAGAAGGACTATGTAGAGCCACTGGGAGAGCTTCAACGATGGTCGTTTTACAAAGCTGTGATAGCGGAGTTTGTCGCCACTCTTCTCTTCCTATATGTCACGGTTGCCACCATCATCGGACACAAGAAGGAAGCTGCTCAGTGTGACGGCGTTGGTGTTCTTGGCATTGTCTGGGCCTTCGGTGGCATGATCTTCATCCTGGTTTACTCCATCGCTGGAATCTCAGGCGGTCACATCAACCCTGCGGTGACTTTCGGGCTGTTGGTTGCGAGGAGGGTGTCACTGCTCCGAACAGTGGCTTACATTGTTGCACAATGCTTAGGAGGCATATGCGGCGTAGGCATTACTACCTCACGCGCGCATGGAGGTGGTGCCAACTCGGTTGCTCCTGATTATTCCAAAGGCGCTGCTTTGGGAGCTGAAATAGTTGGCACTTTTCTTCTGGTCTACACAGATGCTAAGAGAAGCGCACGTGACTCCCATGTGCCTGTATTGGTTCCATTACCAATAGGGTTTGCTGTGTTTATGGTTCACCTGGCCACCATACCTATTACTGGGACTGGGATCAACCCTGCTAGGAGTTTTGCTGCTGCTGTAATCTACAACAATGACAAAGTCTGGGACGACCAGTGGATATTCTGGGTCGGACCATTCATTGGAGCTCTGATAGCAGCAGCTTATAACCAGTACGTACTGAGGGCATCAGCTAGTAAAGCCTTCAGATCGTTCCACAACAATACCACCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

267

Amino Acids

28.7

Weight (kDa)

7.05

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 28 - 244 7.7e-71 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66920
fragaria_vesca FvH4_1g14990 FvH4_1g14990
malus_domestica MD15G1276700.v1.1
prunus_persica Prupe.7G140500_v2.0.a1
pyrus_communis pycom02g13010 pycom15g23960
rosa_chinensis RchiOBHm_Chr2g0104051
rosa_laevigata RLG00000017282
rosa_multiflora Rmu_ssc0000257.1_g000032
rosa_roxburghii Rroxscaffold_2G00139160
rosa_rugosa Rorug02G0119100
rosa_samantha Rh2CG174100 Rh2DG173700
rosa_wichuraiana Rw2G013160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 735
AccB1I GGYRCC 1 cut(s) 425
AccB7I CCANNNNNTGG 1 cut(s) 710
AccI GTMKAC 1 cut(s) 501
AccII CGCG 2 cut(s) 411, 413
AciI CCGC 4 cut(s) 119, 282, 299, 387
AclWI GGATC 1 cut(s) 626
AcoI YGGCCR 1 cut(s) 591
AcuI CTGAAG 1 cut(s) 757
AcvI CACGTG 1 cut(s) 527
AfaI GTAC 2 cut(s) 743, 747
AfiI CCNNNNNNNGG 3 cut(s) 349, 608, 710
AgsI TTSAA 2 cut(s) 7, 89
AjnI CCWGG 2 cut(s) 252, 588
AluBI AGCT 7 cut(s) 85, 110, 188, 473, 716, 731, 761
AluI AGCT 7 cut(s) 85, 110, 188, 473, 716, 731, 761
Alw21I GWGCWC 1 cut(s) 718
AlwI GGATC 1 cut(s) 626
AoxI GGCC 2 cut(s) 228, 591
ApeKI GCWGC 8 cut(s) 46, 49, 188, 461, 641, 644, 725, 728
AspLEI GCGC 4 cut(s) 413, 415, 461, 524
AspS9I GGNCC 2 cut(s) 228, 701
AsuHPI GGTGA 2 cut(s) 313, 578
AvaII GGWCC 1 cut(s) 701
BalI TGGCCA 1 cut(s) 593
BanI GGYRCC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 718
BbrPI CACGTG 1 cut(s) 527
Bbv12I GWGCWC 1 cut(s) 718
BbvI GCAGC 8 cut(s) 58, 61, 175, 448, 628, 631, 737, 740
BccI CCATC 3 cut(s) 87, 173, 272
BceAI ACGGC 1 cut(s) 217
BciT130I CCWGG 2 cut(s) 254, 590
BfaI CTAG 2 cut(s) 630, 762
BfoI RGCGCY 1 cut(s) 462
BisI GCNGC 9 cut(s) 47, 50, 189, 388, 462, 642, 645, 726, 729
BlsI GCNGC 9 cut(s) 48, 51, 190, 389, 463, 643, 646, 727, 730
Bme1390I CCNGG 2 cut(s) 254, 590
Bme18I GGWCC 1 cut(s) 701
BmgT120I GGNCC 2 cut(s) 228, 701
BmiI GGNNCC 2 cut(s) 427, 552
BmrFI CCNGG 2 cut(s) 254, 590
BmrI ACTGGG 3 cut(s) 86, 618, 624
BmsI GCATC 2 cut(s) 499, 764
BmuI ACTGGG 3 cut(s) 86, 618, 624
Bpu10I CCTNAGC 1 cut(s) 373
BsaAI YACGTR 2 cut(s) 527, 745
BsaBI GATNNNNATC 1 cut(s) 248
Bsc4I CCNNNNNNNGG 3 cut(s) 349, 608, 710
Bse1I ACTGG 5 cut(s) 81, 613, 619, 682, 739
Bse8I GATNNNNATC 1 cut(s) 248
BseBI CCWGG 2 cut(s) 254, 590
BseGI GGATG 1 cut(s) 249
BseJI GATNNNNATC 1 cut(s) 248
BseLI CCNNNNNNNGG 3 cut(s) 349, 608, 710
BseMII CTCAG 3 cut(s) 206, 291, 740
BseNI ACTGG 5 cut(s) 81, 613, 619, 682, 739
BsePI GCGCGC 1 cut(s) 411
BseRI GAGGAG 1 cut(s) 340
BseXI GCAGC 8 cut(s) 58, 61, 175, 448, 628, 631, 737, 740
Bsh1236I CGCG 2 cut(s) 411, 413
BshFI GGCC 2 cut(s) 230, 593
BshNI GGYRCC 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 718
BslFI GGGAC 2 cut(s) 625, 689
BslI CCNNNNNNNGG 3 cut(s) 349, 608, 710
BsmFI GGGAC 2 cut(s) 625, 689
BsnI GGCC 2 cut(s) 230, 593
Bsp1286I GDGCHC 1 cut(s) 718
Bsp143I GATC 3 cut(s) 243, 618, 776
BspACI CCGC 4 cut(s) 119, 282, 299, 387
BspANI GGCC 2 cut(s) 230, 593
BspCNI CTCAG 3 cut(s) 205, 290, 741
BspFNI CGCG 2 cut(s) 411, 413
BspLI GGNNCC 2 cut(s) 427, 552
BspPI GGATC 1 cut(s) 626
BspQI GCTCTTC 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 425
BsrI ACTGG 5 cut(s) 81, 613, 619, 682, 739
BssHII GCGCGC 1 cut(s) 411
BssMI GATC 3 cut(s) 243, 618, 776
Bst2UI CCWGG 2 cut(s) 254, 590
Bst4CI ACNGT 2 cut(s) 157, 349
Bst6I CTCTTC 3 cut(s) 36, 141, 146
BstBAI YACGTR 2 cut(s) 527, 745
BstC8I GCNNGC 1 cut(s) 413
BstDEI CTNAG 5 cut(s) 192, 277, 373, 513, 749
BstF5I GGATG 1 cut(s) 249
BstFNI CGCG 2 cut(s) 411, 413
BstH2I RGCGCY 1 cut(s) 462
BstHHI GCGC 4 cut(s) 413, 415, 461, 524
BstKTI GATC 3 cut(s) 246, 621, 779
BstMBI GATC 3 cut(s) 243, 618, 776
BstMWI GCNNNNNNNGC 7 cut(s) 116, 378, 387, 393, 470, 722, 767
BstNI CCWGG 2 cut(s) 254, 590
BstSCI CCNGG 2 cut(s) 252, 588
BstSNI TACGTA 1 cut(s) 745
BstUI CGCG 2 cut(s) 411, 413
BstV1I GCAGC 8 cut(s) 58, 61, 175, 448, 628, 631, 737, 740
BsuRI GGCC 2 cut(s) 230, 593
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 1 cut(s) 249
BtsI GCAGTG 1 cut(s) 335
BtsIMutI CAGTG 5 cut(s) 74, 200, 335, 354, 689
Cac8I GCNNGC 1 cut(s) 413
CfoI GCGC 4 cut(s) 413, 415, 461, 524
Cfr13I GGNCC 2 cut(s) 228, 701
Csp6I GTAC 2 cut(s) 742, 746
CviAII CATG 3 cut(s) 241, 416, 536
CviQI GTAC 2 cut(s) 742, 746
DdeI CTNAG 5 cut(s) 192, 277, 373, 513, 749
DpnI GATC 3 cut(s) 245, 620, 778
DpnII GATC 3 cut(s) 243, 618, 776
EaeI YGGCCR 1 cut(s) 591
Eam1104I CTCTTC 3 cut(s) 36, 141, 146
EarI CTCTTC 3 cut(s) 36, 141, 146
Ecl136II GAGCTC 1 cut(s) 716
Eco105I TACGTA 1 cut(s) 745
Eco24I GRGCYC 1 cut(s) 718
Eco47I GGWCC 1 cut(s) 701
Eco53kI GAGCTC 1 cut(s) 716
Eco57I CTGAAG 1 cut(s) 757
Eco72I CACGTG 1 cut(s) 527
EcoICRI GAGCTC 1 cut(s) 716
EcoRII CCWGG 2 cut(s) 252, 588
EcoT38I GRGCYC 1 cut(s) 718
FaeI CATG 3 cut(s) 244, 419, 539
FaqI GGGAC 2 cut(s) 625, 689
FatI CATG 3 cut(s) 240, 415, 535
FauNDI CATATG 1 cut(s) 383
FblI GTMKAC 1 cut(s) 501
Fnu4HI GCNGC 9 cut(s) 47, 50, 189, 388, 462, 642, 645, 726, 729
FokI GGATG 1 cut(s) 236
FriOI GRGCYC 1 cut(s) 718
Fsp4HI GCNGC 9 cut(s) 47, 50, 189, 388, 462, 642, 645, 726, 729
FspBI CTAG 2 cut(s) 630, 762
GlaI GCGC 4 cut(s) 412, 414, 460, 523
GluI GCNGC 9 cut(s) 47, 50, 189, 388, 462, 642, 645, 726, 729
HaeII RGCGCY 1 cut(s) 462
HaeIII GGCC 2 cut(s) 230, 593
HhaI GCGC 4 cut(s) 413, 415, 461, 524
Hin1II CATG 3 cut(s) 244, 419, 539
Hin6I GCGC 4 cut(s) 411, 413, 459, 522
HinP1I GCGC 4 cut(s) 411, 413, 459, 522
HinfI GANTC 2 cut(s) 273, 530
HphI GGTGA 2 cut(s) 313, 578
Hpy166II GTNNAC 4 cut(s) 37, 259, 502, 586
Hpy188I TCNGA 5 cut(s) 173, 344, 701, 720, 776
Hpy188III TCNNGA 2 cut(s) 38, 443
Hpy8I GTNNAC 4 cut(s) 37, 259, 502, 586
HpyAV CCTTC 5 cut(s) 17, 52, 175, 241, 781
HpyCH4III ACNGT 2 cut(s) 157, 349
HpyCH4IV ACGT 2 cut(s) 526, 744
HpyCH4V TGCA 1 cut(s) 365
HpyF10VI GCNNNNNNNGC 7 cut(s) 116, 378, 387, 393, 470, 722, 767
HpyF3I CTNAG 5 cut(s) 192, 277, 373, 513, 749
HpySE526I ACGT 2 cut(s) 526, 744
Hsp92II CATG 3 cut(s) 244, 419, 539
HspAI GCGC 4 cut(s) 411, 413, 459, 522
Kzo9I GATC 3 cut(s) 243, 618, 776
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 4 cut(s) 345, 445, 470, 713
Lsp1109I GCAGC 8 cut(s) 58, 61, 175, 448, 628, 631, 737, 740
LweI GCATC 2 cut(s) 499, 764
MaeI CTAG 2 cut(s) 630, 762
MaeII ACGT 2 cut(s) 526, 744
MaeIII GTNAC 6 cut(s) 151, 197, 284, 301, 333, 527
MalI GATC 3 cut(s) 245, 620, 778
MboI GATC 3 cut(s) 243, 618, 776
MboII GAAGA 6 cut(s) 32, 53, 128, 133, 238, 485
MhlI GDGCHC 1 cut(s) 718
MlsI TGGCCA 1 cut(s) 593
MluNI TGGCCA 1 cut(s) 593
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 679
MnlI CCTC 7 cut(s) 25, 318, 321, 371, 413, 415, 744
Mox20I TGGCCA 1 cut(s) 593
MscI TGGCCA 1 cut(s) 593
MslI CAYNNNNRTG 1 cut(s) 420
Msp20I TGGCCA 1 cut(s) 593
MspR9I CCNGG 2 cut(s) 254, 590
MvaI CCWGG 2 cut(s) 254, 590
MvnI CGCG 2 cut(s) 411, 413
MwoI GCNNNNNNNGC 7 cut(s) 116, 378, 387, 393, 470, 722, 767
NdeI CATATG 1 cut(s) 383
NdeII GATC 3 cut(s) 243, 618, 776
NlaIII CATG 3 cut(s) 244, 419, 539
NlaIV GGNNCC 2 cut(s) 427, 552
NmuCI GTSAC 6 cut(s) 151, 197, 284, 301, 333, 527
PauI GCGCGC 1 cut(s) 411
PciSI GCTCTTC 1 cut(s) 36
PfeI GAWTC 1 cut(s) 273
PflFI GACNNNGTC 1 cut(s) 668
PflMI CCANNNNNTGG 1 cut(s) 710
PkrI GCNGC 9 cut(s) 48, 51, 190, 389, 463, 643, 646, 727, 730
PleI GAGTC 1 cut(s) 524
PmaCI CACGTG 1 cut(s) 527
PmlI CACGTG 1 cut(s) 527
PpsI GAGTC 1 cut(s) 524
Ppu21I YACGTR 2 cut(s) 527, 745
PsiI TTATAA 1 cut(s) 735
Psp124BI GAGCTC 1 cut(s) 718
Psp6I CCWGG 2 cut(s) 252, 588
PspCI CACGTG 1 cut(s) 527
PspGI CCWGG 2 cut(s) 252, 588
PspN4I GGNNCC 2 cut(s) 427, 552
PspPI GGNCC 2 cut(s) 228, 701
PsyI GACNNNGTC 1 cut(s) 668
PteI GCGCGC 1 cut(s) 411
RsaI GTAC 2 cut(s) 743, 747
RsaNI GTAC 2 cut(s) 742, 746
RseI CAYNNNNRTG 1 cut(s) 420
SacI GAGCTC 1 cut(s) 718
SapI GCTCTTC 1 cut(s) 36
SatI GCNGC 9 cut(s) 47, 50, 189, 388, 462, 642, 645, 726, 729
Sau3AI GATC 3 cut(s) 243, 618, 776
Sau96I GGNCC 2 cut(s) 228, 701
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 2 cut(s) 254, 590
SduI GDGCHC 1 cut(s) 718
SfaNI GCATC 2 cut(s) 499, 764
SinI GGWCC 1 cut(s) 701
SmiMI CAYNNNNRTG 1 cut(s) 420
SnaBI TACGTA 1 cut(s) 745
SsiI CCGC 4 cut(s) 119, 282, 299, 387
SspMI CTAG 2 cut(s) 630, 762
SstI GAGCTC 1 cut(s) 718
StyD4I CCNGG 2 cut(s) 252, 588
TaaI ACNGT 2 cut(s) 157, 349
TaiI ACGT 2 cut(s) 529, 747
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 1 cut(s) 273
TscAI CASTG 5 cut(s) 81, 200, 342, 354, 689
TseFI GTSAC 6 cut(s) 151, 197, 284, 301, 333, 527
TseI GCWGC 8 cut(s) 46, 49, 188, 461, 641, 644, 725, 728
Tsp45I GTSAC 6 cut(s) 151, 197, 284, 301, 333, 527
TspDTI ATGAA 2 cut(s) 238, 697
TspRI CASTG 5 cut(s) 81, 200, 342, 354, 689
Tth111I GACNNNGTC 1 cut(s) 668
Van91I CCANNNNNTGG 1 cut(s) 710
VpaK11BI GGWCC 1 cut(s) 701
XmiI GTMKAC 1 cut(s) 501
XspI CTAG 2 cut(s) 630, 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.