Rroxscaffold_2G00139160

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015973
Physical Location & Seq
Forward (+)
77495420 .. 77498486
3067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139160.1

Sequence Viewer

Length: 1545 bp
ATGGGAAAAGCAGTCTTGCTCCAATTGCTGCTTGGTGTTTTGGCTGTTGTGTTAAGCGCTTCTGTGGTGAAAGCAGAGGACCCATATAAGTACTACACCTGGACAGTGACTTATGGGACTCTTTCTCCTCTGGGTGTTCCCCAACAGGTGATACTTATCAATGGTCAATTTCCTGGGCCTAGACTTGATGTGGTGACCAATGACAACATCATCCTCAACCTCATTAACAAGCTGGACCAGCCTTTTCTGCTAACATGGAATGGGATTAAGCAGAGGAAAAACTCATGGCAAGATGGGGTATTGGGAACCAATTGCCCCATCCCTCCAAACTCAAACTATACCTACAAAATTCAGGCCAAGGATCAGATAGGTACCTTCACATATTTTCCATCAACTAAATTGCACAGAGCTGCTGGAGCGTTTGGAGGTATCAATGTCTATTCGAGACCTCGAATTCCAATCCCCTATCCACTCCCTGAGAGTGATTTCACTTTACTTGTTGGTGATTGGTACAAGACCAGCCATCAGGCTTTGCAGAAATCTTTGGACTCAGGAAAGTCGCTTCCATTCCCCGACGGTCTTCTTATAAATGGCCAGGCTAGTACTACCTTCGGTGGTGATCAAGGCAAAACATATATGTTTAGGGTATCAAATGTGGGATTGTCAACCTCATTAAACTTCAGAATTCAGGGCCATAAATTGAAGCTAGTTGAATTGACCTTAAATCAGCCTCCAAAGGATTACTACATTGTAGCATCCACGCGATTCACTAGGCGTGTTCTTACAGCAACTGCAGTGCTACACTACTCGAACTCTCACACTTCAGTTTCTGGACCTGTACCTGCTGGTCCTAGTTTCCAACTTCACTGGTCTATGCAGCAAGCCAGAACTTTCAGGTGGAATTTGACAGCAAATGCAGCCAGACCTAACCCTCAGGGCTCATACCATTATGGACAGATAGTACCAACAAAGACAATTGTATTGGCCAATTCAGCACCTTTGATAAATGGAACCCTGCGTTACGCATTTAACCAAGTCTCCTACGTTAACCCTGACACCCCATTAAAGCTTGCTGATTACTTTAACATTCCTGGGGTCTTCACCTTTGATGCCATCCAATCCGTTCCCTCTGGTAGCTCTGCAACATTAGCTACACCTGTGTTGCCAGCCTCACTCCACGACTTCCTTGAAATCGTTTTCCAAAACAACGAAAAGACCATGCAATCTGTGCATCTAGATGGTTATGATTTCTGGGCTGTTGGCTATGGTTCTGGACAGTGGCAACCAGCCAAGAGAAAAACCTATAATCTAGTTGATGCTCTAACTAGACATACTGTTCAGGTTTATCCAAACTCTTGGAGTACTATATTGGTGTCACTAGACAACCAAGGAATGTGGAACTTGAGGTCAGCGACATGGGAAAGGCAGTATCTTGGGCAACAGCTCTATCTCAGGGTTTGGAATCCAACCCAAAACCTTGCTAATGAATACAACATTCCTTCAAATGCTCTAATGTGTGGAAAAGCTGTAGGACGACACCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

514

Amino Acids

57.19

Weight (kDa)

9.54

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 36 - 149 1.5e-39 Multicopper oxidase
Cu-oxidase PF00394 162 - 240 5.9e-20 Multicopper oxidase
Cu-oxidase_2 PF07731 352 - 490 4e-31 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66920
fragaria_vesca FvH4_1g14990 FvH4_1g14990
malus_domestica MD15G1276700.v1.1
prunus_persica Prupe.7G140500_v2.0.a1
pyrus_communis pycom02g13010 pycom15g23960
rosa_chinensis RchiOBHm_Chr2g0104051
rosa_laevigata RLG00000017282
rosa_multiflora Rmu_ssc0000257.1_g000032
rosa_roxburghii Rroxscaffold_2G00139160
rosa_rugosa Rorug02G0119100
rosa_samantha Rh2CG174100 Rh2DG173700
rosa_wichuraiana Rw2G013160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 587
Acc36I ACCTGC 1 cut(s) 850
Acc65I GGTACC 1 cut(s) 371
AccB1I GGYRCC 1 cut(s) 371
AccII CGCG 1 cut(s) 763
AclWI GGATC 1 cut(s) 369
AcoI YGGCCR 2 cut(s) 592, 984
AcsI RAATTY 4 cut(s) 348, 453, 684, 901
AcuI CTGAAG 2 cut(s) 664, 807
AfaI GTAC 7 cut(s) 92, 373, 512, 604, 840, 963, 1363
AfeI AGCGCT 1 cut(s) 58
AgsI TTSAA 4 cut(s) 703, 713, 1190, 1503
AjnI CCWGG 4 cut(s) 98, 172, 594, 1090
AluBI AGCT 8 cut(s) 232, 410, 706, 1069, 1137, 1151, 1444, 1526
AluI AGCT 8 cut(s) 232, 410, 706, 1069, 1137, 1151, 1444, 1526
Alw26I GTCTC 2 cut(s) 439, 1042
AlwI GGATC 1 cut(s) 369
AlwNI CAGNNNCTG 2 cut(s) 791, 830
Aor51HI AGCGCT 1 cut(s) 58
AoxI GGCC 5 cut(s) 176, 354, 592, 691, 984
ApeKI GCWGC 4 cut(s) 28, 410, 877, 917
ApoI RAATTY 4 cut(s) 348, 453, 684, 901
Asp718I GGTACC 1 cut(s) 371
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 6 cut(s) 79, 176, 235, 691, 833, 848
AsuHPI GGTGA 6 cut(s) 79, 160, 205, 515, 629, 1093
AvaII GGWCC 4 cut(s) 79, 235, 833, 848
AxyI CCTNAGG 1 cut(s) 933
BalI TGGCCA 2 cut(s) 594, 986
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 941
BbsI GAAGAC 2 cut(s) 572, 1090
BbvI GCAGC 4 cut(s) 15, 397, 889, 929
BccI CCATC 6 cut(s) 287, 326, 397, 531, 1121, 1232
BciT130I CCWGG 4 cut(s) 100, 174, 596, 1092
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 2 cut(s) 439, 1042
BfaI CTAG 9 cut(s) 180, 600, 707, 771, 852, 1235, 1310, 1326, 1379
BfmI CTRYAG 2 cut(s) 792, 1527
BfoI RGCGCY 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 850
BisI GCNGC 4 cut(s) 29, 411, 878, 918
BlsI GCNGC 4 cut(s) 30, 412, 879, 919
BmcAI AGTACT 3 cut(s) 92, 604, 1363
Bme1390I CCNGG 4 cut(s) 100, 174, 596, 1092
Bme18I GGWCC 4 cut(s) 79, 235, 833, 848
BmgT120I GGNCC 6 cut(s) 79, 176, 235, 691, 833, 848
BmiI GGNNCC 4 cut(s) 81, 307, 373, 1012
BmrFI CCNGG 4 cut(s) 100, 174, 596, 1092
BmsI GCATC 4 cut(s) 764, 1099, 1240, 1306
BpiI GAAGAC 2 cut(s) 572, 1090
BpmI CTGGAG 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 1423
BsaBI GATNNNNATC 1 cut(s) 155
BsaI GGTCTC 1 cut(s) 439
BsaJI CCNNGG 4 cut(s) 173, 357, 1091, 1387
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 1022, 1052
Bse1I ACTGG 1 cut(s) 872
Bse21I CCTNAGG 1 cut(s) 933
Bse8I GATNNNNATC 1 cut(s) 155
BseBI CCWGG 4 cut(s) 100, 174, 596, 1092
BseDI CCNNGG 4 cut(s) 173, 357, 1091, 1387
BseGI GGATG 4 cut(s) 210, 318, 755, 1113
BseJI GATNNNNATC 1 cut(s) 155
BseMII CTCAG 4 cut(s) 468, 564, 947, 1465
BseNI ACTGG 1 cut(s) 872
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 4 cut(s) 15, 397, 889, 929
Bsh1236I CGCG 1 cut(s) 763
BshFI GGCC 5 cut(s) 178, 356, 594, 693, 986
BshNI GGYRCC 1 cut(s) 371
BslFI GGGAC 1 cut(s) 130
BsmAI GTCTC 2 cut(s) 439, 1042
BsmFI GGGAC 1 cut(s) 130
BsnI GGCC 5 cut(s) 178, 356, 594, 693, 986
Bso31I GGTCTC 1 cut(s) 439
Bsp1286I GDGCHC 1 cut(s) 941
Bsp143I GATC 2 cut(s) 361, 619
BspANI GGCC 5 cut(s) 178, 356, 594, 693, 986
BspCNI CTCAG 4 cut(s) 469, 563, 946, 1464
BspFNI CGCG 1 cut(s) 763
BspLI GGNNCC 4 cut(s) 81, 307, 373, 1012
BspMAI CTGCAG 1 cut(s) 796
BspMI ACCTGC 1 cut(s) 850
BspPI GGATC 1 cut(s) 369
BspT107I GGYRCC 1 cut(s) 371
BspTNI GGTCTC 1 cut(s) 439
BsrI ACTGG 1 cut(s) 872
BssECI CCNNGG 4 cut(s) 173, 357, 1091, 1387
BssMI GATC 2 cut(s) 361, 619
BssT1I CCWWGG 2 cut(s) 357, 1387
Bst2UI CCWGG 4 cut(s) 100, 174, 596, 1092
Bst4CI ACNGT 4 cut(s) 106, 578, 1278, 1336
BstAPI GCANNNNNTGC 1 cut(s) 1228
BstC8I GCNNGC 3 cut(s) 882, 1071, 1167
BstDEI CTNAG 5 cut(s) 477, 550, 933, 1451, 1542
BstEII GGTNACC 1 cut(s) 193
BstF5I GGATG 4 cut(s) 210, 318, 755, 1113
BstFNI CGCG 1 cut(s) 763
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 2 cut(s) 364, 622
BstMAI GTCTC 2 cut(s) 439, 1042
BstMBI GATC 2 cut(s) 361, 619
BstMWI GCNNNNNNNGC 8 cut(s) 25, 238, 247, 416, 917, 992, 1148, 1228
BstNI CCWGG 4 cut(s) 100, 174, 596, 1092
BstPI GGTNACC 1 cut(s) 193
BstSCI CCNGG 4 cut(s) 98, 172, 594, 1090
BstSFI CTRYAG 2 cut(s) 792, 1527
BstUI CGCG 1 cut(s) 763
BstV1I GCAGC 4 cut(s) 15, 397, 889, 929
BstV2I GAAGAC 2 cut(s) 572, 1090
BstXI CCANNNNNNTGG 1 cut(s) 1356
Bsu36I CCTNAGG 1 cut(s) 933
BsuRI GGCC 5 cut(s) 178, 356, 594, 693, 986
BtsCI GGATG 4 cut(s) 210, 318, 755, 1113
BtsI GCAGTG 1 cut(s) 801
BtsIMutI CAGTG 4 cut(s) 111, 801, 865, 1283
BveI ACCTGC 1 cut(s) 850
Cac8I GCNNGC 3 cut(s) 882, 1071, 1167
CaiI CAGNNNCTG 2 cut(s) 791, 830
CfoI GCGC 1 cut(s) 59
Cfr13I GGNCC 6 cut(s) 79, 176, 235, 691, 833, 848
Csp6I GTAC 7 cut(s) 91, 372, 511, 603, 839, 962, 1362
CspCI CAANNNNNGTGG 2 cut(s) 1376, 1411
CviAII CATG 4 cut(s) 255, 285, 1219, 1416
CviQI GTAC 7 cut(s) 91, 372, 511, 603, 839, 962, 1362
DdeI CTNAG 5 cut(s) 477, 550, 933, 1451, 1542
DpnI GATC 2 cut(s) 363, 621
DpnII GATC 2 cut(s) 361, 619
EaeI YGGCCR 2 cut(s) 592, 984
Eco130I CCWWGG 2 cut(s) 357, 1387
Eco24I GRGCYC 1 cut(s) 941
Eco31I GGTCTC 1 cut(s) 439
Eco47I GGWCC 4 cut(s) 79, 235, 833, 848
Eco47III AGCGCT 1 cut(s) 58
Eco57I CTGAAG 2 cut(s) 664, 807
Eco81I CCTNAGG 1 cut(s) 933
Eco91I GGTNACC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 79
EcoO65I GGTNACC 1 cut(s) 193
EcoRI GAATTC 2 cut(s) 453, 684
EcoRII CCWGG 4 cut(s) 98, 172, 594, 1090
EcoT14I CCWWGG 2 cut(s) 357, 1387
EcoT38I GRGCYC 1 cut(s) 941
ErhI CCWWGG 2 cut(s) 357, 1387
FaeI CATG 4 cut(s) 258, 288, 1222, 1419
FaqI GGGAC 1 cut(s) 130
FatI CATG 4 cut(s) 254, 284, 1218, 1415
FbaI TGATCA 1 cut(s) 619
Fnu4HI GCNGC 4 cut(s) 29, 411, 878, 918
FokI GGATG 4 cut(s) 197, 305, 742, 1100
FriOI GRGCYC 1 cut(s) 941
Fsp4HI GCNGC 4 cut(s) 29, 411, 878, 918
FspBI CTAG 9 cut(s) 180, 600, 707, 771, 852, 1235, 1310, 1326, 1379
GlaI GCGC 1 cut(s) 58
GluI GCNGC 4 cut(s) 29, 411, 878, 918
GsuI CTGGAG 1 cut(s) 435
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 5 cut(s) 178, 356, 594, 693, 986
HhaI GCGC 1 cut(s) 59
Hin1II CATG 4 cut(s) 258, 288, 1222, 1419
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HincII GTYRAC 2 cut(s) 666, 1048
HindII GTYRAC 2 cut(s) 666, 1048
HindIII AAGCTT 1 cut(s) 1067
HinfI GANTC 4 cut(s) 118, 548, 765, 1462
HpaI GTTAAC 1 cut(s) 1048
HphI GGTGA 6 cut(s) 79, 160, 205, 515, 629, 1093
Hpy166II GTNNAC 2 cut(s) 666, 1048
Hpy188I TCNGA 2 cut(s) 366, 683
Hpy188III TCNNGA 5 cut(s) 444, 552, 831, 1235, 1272
Hpy8I GTNNAC 2 cut(s) 666, 1048
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 3 cut(s) 385, 619, 1509
HpyCH4III ACNGT 4 cut(s) 106, 578, 1278, 1336
HpyCH4IV ACGT 1 cut(s) 1044
HpyCH4V TGCA 8 cut(s) 403, 535, 794, 877, 917, 1142, 1222, 1231
HpyF10VI GCNNNNNNNGC 8 cut(s) 25, 238, 247, 416, 917, 992, 1148, 1228
HpyF3I CTNAG 5 cut(s) 477, 550, 933, 1451, 1542
HpySE526I ACGT 1 cut(s) 1044
Hsp92II CATG 4 cut(s) 258, 288, 1222, 1419
HspAI GCGC 1 cut(s) 57
KpnI GGTACC 1 cut(s) 375
Ksp22I TGATCA 1 cut(s) 619
KspAI GTTAAC 1 cut(s) 1048
Kzo9I GATC 2 cut(s) 361, 619
LmnI GCTCC 2 cut(s) 24, 416
Lsp1109I GCAGC 4 cut(s) 15, 397, 889, 929
LweI GCATC 4 cut(s) 764, 1099, 1240, 1306
MaeI CTAG 9 cut(s) 180, 600, 707, 771, 852, 1235, 1310, 1326, 1379
MaeII ACGT 1 cut(s) 1044
MaeIII GTNAC 4 cut(s) 106, 193, 1019, 1374
MalI GATC 2 cut(s) 363, 621
MboI GATC 2 cut(s) 361, 619
MboII GAAGA 2 cut(s) 572, 1090
MfeI CAATTG 3 cut(s) 23, 310, 975
MhlI GDGCHC 1 cut(s) 941
MlsI TGGCCA 2 cut(s) 594, 986
MluNI TGGCCA 2 cut(s) 594, 986
MlyI GAGTC 2 cut(s) 112, 542
MmeI TCCRAC 2 cut(s) 883, 1490
Mox20I TGGCCA 2 cut(s) 594, 986
MscI TGGCCA 2 cut(s) 594, 986
MseI TTAA 9 cut(s) 53, 225, 267, 674, 722, 1029, 1047, 1064, 1083
MslI CAYNNNNRTG 1 cut(s) 1236
Msp20I TGGCCA 2 cut(s) 594, 986
MspR9I CCNGG 4 cut(s) 100, 174, 596, 1092
MunI CAATTG 3 cut(s) 23, 310, 975
MvaI CCWGG 4 cut(s) 100, 174, 596, 1092
MvnI CGCG 1 cut(s) 763
MwoI GCNNNNNNNGC 8 cut(s) 25, 238, 247, 416, 917, 992, 1148, 1228
NdeII GATC 2 cut(s) 361, 619
NlaIII CATG 4 cut(s) 258, 288, 1222, 1419
NlaIV GGNNCC 4 cut(s) 81, 307, 373, 1012
NmuCI GTSAC 3 cut(s) 106, 193, 1374
PfeI GAWTC 2 cut(s) 765, 1462
PkrI GCNGC 4 cut(s) 30, 412, 879, 919
PleI GAGTC 2 cut(s) 112, 542
PpsI GAGTC 2 cut(s) 112, 542
PpuMI RGGWCCY 1 cut(s) 79
PsiI TTATAA 1 cut(s) 587
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 4 cut(s) 98, 172, 594, 1090
PspEI GGTNACC 1 cut(s) 193
PspGI CCWGG 4 cut(s) 98, 172, 594, 1090
PspN4I GGNNCC 4 cut(s) 81, 307, 373, 1012
PspPI GGNCC 6 cut(s) 79, 176, 235, 691, 833, 848
PspPPI RGGWCCY 1 cut(s) 79
PstI CTGCAG 1 cut(s) 796
PstNI CAGNNNCTG 2 cut(s) 791, 830
RsaI GTAC 7 cut(s) 92, 373, 512, 604, 840, 963, 1363
RsaNI GTAC 7 cut(s) 91, 372, 511, 603, 839, 962, 1362
RseI CAYNNNNRTG 1 cut(s) 1236
SaqAI TTAA 9 cut(s) 53, 225, 267, 674, 722, 1029, 1047, 1064, 1083
SatI GCNGC 4 cut(s) 29, 411, 878, 918
Sau3AI GATC 2 cut(s) 361, 619
Sau96I GGNCC 6 cut(s) 79, 176, 235, 691, 833, 848
ScaI AGTACT 3 cut(s) 92, 604, 1363
SchI GAGTC 2 cut(s) 112, 542
ScrFI CCNGG 4 cut(s) 100, 174, 596, 1092
SduI GDGCHC 1 cut(s) 941
SfaNI GCATC 4 cut(s) 764, 1099, 1240, 1306
SfcI CTRYAG 2 cut(s) 792, 1527
SinI GGWCC 4 cut(s) 79, 235, 833, 848
SmiMI CAYNNNNRTG 1 cut(s) 1236
SmlI CTYRAG 1 cut(s) 1402
SmoI CTYRAG 1 cut(s) 1402
SspMI CTAG 9 cut(s) 180, 600, 707, 771, 852, 1235, 1310, 1326, 1379
StyD4I CCNGG 4 cut(s) 98, 172, 594, 1090
StyI CCWWGG 2 cut(s) 357, 1387
TaaI ACNGT 4 cut(s) 106, 578, 1278, 1336
TaiI ACGT 1 cut(s) 1047
TaqI TCGA 3 cut(s) 443, 451, 809
TatI WGTACW 3 cut(s) 90, 602, 1361
TfiI GAWTC 2 cut(s) 765, 1462
Tru1I TTAA 9 cut(s) 53, 225, 267, 674, 722, 1029, 1047, 1064, 1083
Tru9I TTAA 9 cut(s) 53, 225, 267, 674, 722, 1029, 1047, 1064, 1083
TscAI CASTG 4 cut(s) 111, 801, 872, 1283
TseFI GTSAC 3 cut(s) 106, 193, 1374
TseI GCWGC 4 cut(s) 28, 410, 877, 917
Tsp45I GTSAC 3 cut(s) 106, 193, 1374
TspDTI ATGAA 1 cut(s) 1500
TspGWI ACGGA 1 cut(s) 1111
TspRI CASTG 4 cut(s) 111, 801, 872, 1283
VpaK11BI GGWCC 4 cut(s) 79, 235, 833, 848
XapI RAATTY 4 cut(s) 348, 453, 684, 901
XbaI TCTAGA 1 cut(s) 1234
XcmI CCANNNNNNNNNTGG 1 cut(s) 29
XspI CTAG 9 cut(s) 180, 600, 707, 771, 852, 1235, 1310, 1326, 1379
ZrmI AGTACT 3 cut(s) 92, 604, 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.