pycom15g23960

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015973
Physical Location & Seq
Reverse (-)
18096041 .. 18098713
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g23960.1

Sequence Viewer

Length: 1626 bp
ATGGAGCAAAAAGCAGGCTTCTTGCTCCACTTGGTTTGTGGGGTTTTGGCAGTGCTTCTGAGTTCTTCTGTGGTGAAAGCAGAAGACCCATATAAGTTCTACACTTGGACTGTGACTTATGGGACTCTTTCTCCTCTTGGTGTTCCTCAACAGGTAATTCTCATCAATGGCCAATTTCCTGGCCCTAGACTTGACGTTGTCACCAATGACAACATTATCCTCAACCTAATTAACAAGCTGGACCAACCTTTCCTCTTAACCTGGAATGGGATTAAACAGAGGAAAAACTCATGGCAAGATGGGGTTTTGGGAACCAATTGTCCCATCCCGCCGAACTCAAACTACACCTACAAGTTTCAGACCAAGGATCAGATAGGTTCCTTCACATATTTCCCATCAACTCAATTTCACAAAGCTGCTGGAGGGTTTGGATCGATCAATGTCTACGCAAGACCTCGAATCCCAGTCCCTTACCCAACCCCTGATGGGGATTTCACTTTGCTTGCTGGCGACTGGTACAAGAGCAGCCACCAGGCCTTACAGCAATCTTTGGACTCCGGAAAATCTCTTCCTTTTCCTGCCGGTGTTCTTATAAATGGCCAAACTAGTAACACTTTCAGTGGTGACCAAGGAAAAACATATATGTTCAGGATCTCAAATGTCGGCTTGTCAACCTCATTGAACTTCCGGATTGAAGGTCACAAACTGAAGTTAGTTGAATGCGAAGGATCTCACACAATTCAGAACTCATATGACTCCCTTGACATACATGTTGGCCAATCAGTCTCTGTCCTAGTAACCTTAAATCAGGCTCCAAAGGACTACTACATTGTCGCATCCACACGATTCACTAGGCTTGTTCTTACGGCTACTTCAGTGCTACACTACACAAACTCGCACACCTCCGTTTCTGGACCTGTGCCGGCCGGTCCTACTGGCCAAATACACTGGTCTATGGAGCAAGCCAGAACTTTCAGGTGGAATCTGACAGCAAATGCAGCCAGGCCTAATCCTCAGGGTTCATTCCATTACGGCAAGGTCACACCGACAAAGACAATTGTGTTAGCCAATTCAGCACCATTGATAAATGGAAAGCAGCGATACGCATTCAACAGGGTCTCTTATGTTAACCCGGATACCCCTGTAAAGCTTGCTGATTACTTCAACATCCCTGGAGTCTTCAGCTTAAACTCCATCCAAACCGCTCCCTCAGATGGCTCTGCATCCTTAGCTACCCCTGTTATGCCAGTCTCACACCATGACTTCATTGAAATTGTTTTCCAAAACAATGAAAAGAGTGTCCAATCCATGCATTTAGATGGTTATGATTTCTGGGTTGTTGGTTATGGTTCCGGACCATGGACTCCAGCCAAGAGAACAACCTACAATCTCGTTGATACTCTTACTAGACATACTGCTCAGGTGTATCCAAACTCTTGGACAACTATTTTGGCGTCACTTGACAACCAAGGTATGTGGAACTTGAGGTCTGCTATATGGGAAAGGCAGTATCTTGGGCAACAGTTCTATCTCAGGGTTTGGAATCCAGTCCAAAACCTTGCTAACGAATACACCATTCCTTCAAATGCTCTGCTGTGTGGAAAAGCTGTAGGACGACACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

542

Amino Acids

60.01

Weight (kDa)

9.14

Isoelectric Point (pI)

36.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 38 - 151 1.5e-39 Multicopper oxidase
Cu-oxidase PF00394 164 - 298 1.4e-40 Multicopper oxidase
Cu-oxidase_2 PF07731 379 - 517 3e-33 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66920
fragaria_vesca FvH4_1g14990 FvH4_1g14990
malus_domestica MD15G1276700.v1.1
prunus_persica Prupe.7G140500_v2.0.a1
pyrus_communis pycom02g13010 pycom15g23960
rosa_chinensis RchiOBHm_Chr2g0104051
rosa_laevigata RLG00000017282
rosa_multiflora Rmu_ssc0000257.1_g000032
rosa_roxburghii Rroxscaffold_2G00139160
rosa_rugosa Rorug02G0119100
rosa_samantha Rh2CG174100 Rh2DG173700
rosa_wichuraiana Rw2G013160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 593
AccBSI CCGCTC 1 cut(s) 1205
AccI GTMKAC 1 cut(s) 444
AccIII TCCGGA 3 cut(s) 557, 687, 1352
AciI CCGC 2 cut(s) 329, 1203
AclWI GGATC 4 cut(s) 375, 439, 659, 736
AcoI YGGCCR 5 cut(s) 169, 598, 775, 924, 937
AcuI CTGAAG 3 cut(s) 728, 858, 1165
AcyI GRCGYC 1 cut(s) 1454
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 5 cut(s) 267, 486, 487, 1214, 1359
AflIII ACRYGT 1 cut(s) 769
AgsI TTSAA 7 cut(s) 682, 695, 719, 1111, 1165, 1271, 1584
AhlI ACTAGT 1 cut(s) 605
AjnI CCWGG 5 cut(s) 178, 260, 531, 1001, 1171
AloI GAACNNNNNNTCC 2 cut(s) 304, 336
AluBI AGCT 6 cut(s) 238, 416, 1150, 1185, 1232, 1607
AluI AGCT 6 cut(s) 238, 416, 1150, 1185, 1232, 1607
Alw26I GTCTC 3 cut(s) 790, 1123, 1255
AlwI GGATC 4 cut(s) 375, 439, 659, 736
AlwNI CAGNNNCTG 1 cut(s) 788
Aor13HI TCCGGA 3 cut(s) 557, 687, 1352
AoxI GGCC 8 cut(s) 169, 181, 534, 598, 775, 924, 937, 1004
ApeKI GCWGC 4 cut(s) 416, 525, 998, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 1432, 1464
AspS9I GGNCC 5 cut(s) 182, 241, 914, 929, 1355
AsuC2I CCSGG 1 cut(s) 1133
AsuHPI GGTGA 3 cut(s) 85, 193, 635
AvaII GGWCC 4 cut(s) 241, 914, 929, 1355
AxyI CCTNAGG 1 cut(s) 1014
BaeI ACNNNNGTAYC 2 cut(s) 1128, 1161
BalI TGGCCA 4 cut(s) 171, 600, 777, 939
BbsI GAAGAC 2 cut(s) 90, 1171
BbvI GCAGC 4 cut(s) 403, 537, 1010, 1108
BccI CCATC 7 cut(s) 293, 332, 403, 479, 1202, 1208, 1313
BceAI ACGGC 2 cut(s) 882, 1048
BciT130I CCWGG 5 cut(s) 180, 262, 533, 1003, 1173
BciVI GTATCC 2 cut(s) 1129, 1437
BcnI CCSGG 1 cut(s) 1133
BcoDI GTCTC 3 cut(s) 790, 1123, 1255
BcuI ACTAGT 1 cut(s) 605
BfaI CTAG 5 cut(s) 186, 606, 794, 852, 1407
BfmI CTRYAG 1 cut(s) 1608
BfuI GTATCC 2 cut(s) 1129, 1437
BisI GCNGC 4 cut(s) 417, 526, 999, 1097
BlsI GCNGC 4 cut(s) 418, 527, 1000, 1098
Bme1390I CCNGG 6 cut(s) 180, 262, 533, 1003, 1133, 1173
Bme18I GGWCC 4 cut(s) 241, 914, 929, 1355
BmgT120I GGNCC 5 cut(s) 182, 241, 914, 929, 1355
BmiI GGNNCC 4 cut(s) 313, 379, 813, 1351
BmrFI CCNGG 6 cut(s) 180, 262, 533, 1003, 1133, 1173
BmrI ACTGGG 1 cut(s) 458
BmsI GCATC 2 cut(s) 845, 1232
BmuI ACTGGG 1 cut(s) 458
BpiI GAAGAC 2 cut(s) 90, 1171
BpmI CTGGAG 3 cut(s) 441, 1194, 1350
Bpu10I CCTNAGC 2 cut(s) 1228, 1419
BpuEI CTTGAG 1 cut(s) 1504
BpuMI CCSGG 1 cut(s) 1133
Bsa29I ATCGAT 1 cut(s) 434
BsaHI GRCGYC 1 cut(s) 1454
BsaI GGTCTC 1 cut(s) 1123
BsaJI CCNNGG 5 cut(s) 363, 628, 1171, 1358, 1468
BsaWI WCCGGW 3 cut(s) 557, 687, 1352
BsaXI ACNNNNNCTCC 2 cut(s) 115, 145
Bsc4I CCNNNNNNNGG 5 cut(s) 267, 486, 487, 1214, 1359
Bse118I RCCGGY 3 cut(s) 581, 922, 926
Bse1I ACTGG 6 cut(s) 464, 518, 940, 953, 1247, 1547
Bse21I CCTNAGG 1 cut(s) 1014
BseAI TCCGGA 3 cut(s) 557, 687, 1352
BseBI CCWGG 5 cut(s) 180, 262, 533, 1003, 1173
BseCI ATCGAT 1 cut(s) 434
BseDI CCNNGG 5 cut(s) 363, 628, 1171, 1358, 1468
BseGI GGATG 5 cut(s) 324, 836, 1167, 1194, 1223
BseLI CCNNNNNNNGG 5 cut(s) 267, 486, 487, 1214, 1359
BseMII CTCAG 5 cut(s) 50, 1028, 1224, 1433, 1546
BseNI ACTGG 6 cut(s) 464, 518, 940, 953, 1247, 1547
BseRI GAGGAG 1 cut(s) 123
BseX3I CGGCCG 1 cut(s) 924
BseXI GCAGC 4 cut(s) 403, 537, 1010, 1108
Bsh1285I CGRYCG 1 cut(s) 927
BshFI GGCC 8 cut(s) 171, 183, 536, 600, 777, 926, 939, 1006
BshVI ATCGAT 1 cut(s) 434
BsiEI CGRYCG 1 cut(s) 927
BsiSI CCGG 7 cut(s) 558, 582, 688, 923, 927, 1133, 1353
BslFI GGGAC 3 cut(s) 136, 306, 452
BslI CCNNNNNNNGG 5 cut(s) 267, 486, 487, 1214, 1359
BsmAI GTCTC 3 cut(s) 790, 1123, 1255
BsmFI GGGAC 3 cut(s) 136, 306, 452
BsmI GAATGC 2 cut(s) 725, 1106
BsnI GGCC 8 cut(s) 171, 183, 536, 600, 777, 926, 939, 1006
Bso31I GGTCTC 1 cut(s) 1123
Bsp13I TCCGGA 3 cut(s) 557, 687, 1352
Bsp143I GATC 5 cut(s) 367, 431, 435, 651, 728
Bsp19I CCATGG 1 cut(s) 1358
BspACI CCGC 2 cut(s) 329, 1203
BspANI GGCC 8 cut(s) 171, 183, 536, 600, 777, 926, 939, 1006
BspCNI CTCAG 5 cut(s) 51, 1027, 1223, 1432, 1545
BspDI ATCGAT 1 cut(s) 434
BspEI TCCGGA 3 cut(s) 557, 687, 1352
BspLI GGNNCC 4 cut(s) 313, 379, 813, 1351
BspPI GGATC 4 cut(s) 375, 439, 659, 736
BspTNI GGTCTC 1 cut(s) 1123
BsrBI CCGCTC 1 cut(s) 1205
BsrFI RCCGGY 3 cut(s) 581, 922, 926
BsrI ACTGG 6 cut(s) 464, 518, 940, 953, 1247, 1547
BssAI RCCGGY 3 cut(s) 581, 922, 926
BssECI CCNNGG 5 cut(s) 363, 628, 1171, 1358, 1468
BssMI GATC 5 cut(s) 367, 431, 435, 651, 728
BssNI GRCGYC 1 cut(s) 1454
BssT1I CCWWGG 4 cut(s) 363, 628, 1358, 1468
Bst2UI CCWGG 5 cut(s) 180, 262, 533, 1003, 1173
Bst4CI ACNGT 2 cut(s) 112, 1524
Bst6I CTCTTC 1 cut(s) 573
BstACI GRCGYC 1 cut(s) 1454
BstC8I GCNNGC 6 cut(s) 16, 504, 508, 924, 963, 1152
BstDEI CTNAG 6 cut(s) 59, 1014, 1210, 1228, 1419, 1532
BstDSI CCRYGG 1 cut(s) 1358
BstEII GGTNACC 1 cut(s) 623
BstF5I GGATG 5 cut(s) 324, 836, 1167, 1194, 1223
BstKTI GATC 5 cut(s) 370, 434, 438, 654, 731
BstMAI GTCTC 3 cut(s) 790, 1123, 1255
BstMBI GATC 5 cut(s) 367, 431, 435, 651, 728
BstMCI CGRYCG 1 cut(s) 927
BstMWI GCNNNNNNNGC 3 cut(s) 998, 1073, 1229
BstNI CCWGG 5 cut(s) 180, 262, 533, 1003, 1173
BstNSI RCATGY 1 cut(s) 773
BstPI GGTNACC 1 cut(s) 623
BstSCI CCNGG 6 cut(s) 178, 260, 531, 1001, 1131, 1171
BstSFI CTRYAG 1 cut(s) 1608
BstV1I GCAGC 4 cut(s) 403, 537, 1010, 1108
BstV2I GAAGAC 2 cut(s) 90, 1171
BstX2I RGATCY 2 cut(s) 651, 728
BstXI CCANNNNNNTGG 2 cut(s) 179, 1437
BstYI RGATCY 2 cut(s) 651, 728
BstZI CGGCCG 1 cut(s) 924
Bsu15I ATCGAT 1 cut(s) 434
Bsu36I CCTNAGG 1 cut(s) 1014
BsuI GTATCC 2 cut(s) 1129, 1437
BsuRI GGCC 8 cut(s) 171, 183, 536, 600, 777, 926, 939, 1006
BsuTUI ATCGAT 1 cut(s) 434
BtgI CCRYGG 1 cut(s) 1358
BtsCI GGATG 5 cut(s) 324, 836, 1167, 1194, 1223
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 4 cut(s) 57, 625, 882, 946
Cac8I GCNNGC 6 cut(s) 16, 504, 508, 924, 963, 1152
CaiI CAGNNNCTG 1 cut(s) 788
Cfr10I RCCGGY 3 cut(s) 581, 922, 926
Cfr13I GGNCC 5 cut(s) 182, 241, 914, 929, 1355
ClaI ATCGAT 1 cut(s) 434
CseI GACGC 1 cut(s) 1443
Csp6I GTAC 1 cut(s) 517
CspCI CAANNNNNGTGG 4 cut(s) 17, 52, 1457, 1492
CviAII CATG 5 cut(s) 291, 770, 1259, 1309, 1359
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 6 cut(s) 59, 1014, 1210, 1228, 1419, 1532
DpnI GATC 5 cut(s) 369, 433, 437, 653, 730
DpnII GATC 5 cut(s) 367, 431, 435, 651, 728
EaeI YGGCCR 5 cut(s) 169, 598, 775, 924, 937
EagI CGGCCG 1 cut(s) 924
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
EclXI CGGCCG 1 cut(s) 924
Eco130I CCWWGG 4 cut(s) 363, 628, 1358, 1468
Eco147I AGGCCT 2 cut(s) 536, 1006
Eco31I GGTCTC 1 cut(s) 1123
Eco47I GGWCC 4 cut(s) 241, 914, 929, 1355
Eco52I CGGCCG 1 cut(s) 924
Eco57I CTGAAG 3 cut(s) 728, 858, 1165
Eco81I CCTNAGG 1 cut(s) 1014
Eco91I GGTNACC 1 cut(s) 623
EcoO65I GGTNACC 1 cut(s) 623
EcoRII CCWGG 5 cut(s) 178, 260, 531, 1001, 1171
EcoT14I CCWWGG 4 cut(s) 363, 628, 1358, 1468
EcoT22I ATGCAT 1 cut(s) 1314
ErhI CCWWGG 4 cut(s) 363, 628, 1358, 1468
FaeI CATG 5 cut(s) 294, 773, 1262, 1312, 1362
FaqI GGGAC 3 cut(s) 136, 306, 452
FatI CATG 5 cut(s) 290, 769, 1258, 1308, 1358
FauI CCCGC 1 cut(s) 336
FauNDI CATATG 1 cut(s) 751
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 4 cut(s) 417, 526, 999, 1097
FokI GGATG 5 cut(s) 311, 823, 1154, 1181, 1210
Fsp4HI GCNGC 4 cut(s) 417, 526, 999, 1097
FspBI CTAG 5 cut(s) 186, 606, 794, 852, 1407
GluI GCNGC 4 cut(s) 417, 526, 999, 1097
GsuI CTGGAG 3 cut(s) 441, 1194, 1350
HaeIII GGCC 8 cut(s) 171, 183, 536, 600, 777, 926, 939, 1006
HapII CCGG 7 cut(s) 558, 582, 688, 923, 927, 1133, 1353
HgaI GACGC 1 cut(s) 1443
Hin1I GRCGYC 1 cut(s) 1454
Hin1II CATG 5 cut(s) 294, 773, 1262, 1312, 1362
HincII GTYRAC 2 cut(s) 672, 1129
HindII GTYRAC 2 cut(s) 672, 1129
HindIII AAGCTT 1 cut(s) 1148
HinfI GANTC 9 cut(s) 124, 459, 554, 755, 846, 982, 1176, 1363, 1543
HpaI GTTAAC 1 cut(s) 1129
HpaII CCGG 7 cut(s) 558, 582, 688, 923, 927, 1133, 1353
HphI GGTGA 3 cut(s) 85, 193, 635
Hpy166II GTNNAC 3 cut(s) 445, 672, 1129
Hpy188I TCNGA 6 cut(s) 60, 360, 372, 744, 987, 1213
Hpy188III TCNNGA 5 cut(s) 558, 649, 688, 912, 1353
Hpy8I GTNNAC 3 cut(s) 445, 672, 1129
HpyAV CCTTC 4 cut(s) 391, 689, 719, 1590
HpyCH4III ACNGT 2 cut(s) 112, 1524
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 3 cut(s) 998, 1223, 1312
HpyF10VI GCNNNNNNNGC 3 cut(s) 998, 1073, 1229
HpyF3I CTNAG 6 cut(s) 59, 1014, 1210, 1228, 1419, 1532
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 1 cut(s) 1454
Hsp92II CATG 5 cut(s) 294, 773, 1262, 1312, 1362
Kpn2I TCCGGA 3 cut(s) 557, 687, 1352
KroI GCCGGC 1 cut(s) 922
KroNI GCCGGC 1 cut(s) 924
KspAI GTTAAC 1 cut(s) 1129
Kzo9I GATC 5 cut(s) 367, 431, 435, 651, 728
LmnI GCTCC 5 cut(s) 4, 30, 817, 958, 1210
Lsp1109I GCAGC 4 cut(s) 403, 537, 1010, 1108
LweI GCATC 2 cut(s) 845, 1232
MaeI CTAG 5 cut(s) 186, 606, 794, 852, 1407
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 8 cut(s) 112, 199, 608, 623, 698, 796, 1039, 1455
MalI GATC 5 cut(s) 369, 433, 437, 653, 730
MbiI CCGCTC 1 cut(s) 1205
MboI GATC 5 cut(s) 367, 431, 435, 651, 728
MboII GAAGA 4 cut(s) 57, 95, 560, 1171
MfeI CAATTG 2 cut(s) 316, 1056
MflI RGATCY 2 cut(s) 651, 728
MlsI TGGCCA 4 cut(s) 171, 600, 777, 939
MluCI AATT 9 cut(s) 156, 173, 228, 316, 404, 738, 1056, 1069, 1272
MluNI TGGCCA 4 cut(s) 171, 600, 777, 939
MlyI GAGTC 5 cut(s) 118, 548, 749, 1185, 1357
Mox20I TGGCCA 4 cut(s) 171, 600, 777, 939
Mph1103I ATGCAT 1 cut(s) 1314
MroI TCCGGA 3 cut(s) 557, 687, 1352
MroNI GCCGGC 1 cut(s) 922
MscI TGGCCA 4 cut(s) 171, 600, 777, 939
MseI TTAA 6 cut(s) 231, 257, 273, 803, 1128, 1187
MslI CAYNNNNRTG 1 cut(s) 1317
Msp20I TGGCCA 4 cut(s) 171, 600, 777, 939
MspI CCGG 7 cut(s) 558, 582, 688, 923, 927, 1133, 1353
MspR9I CCNGG 6 cut(s) 180, 262, 533, 1003, 1133, 1173
MunI CAATTG 2 cut(s) 316, 1056
Mva1269I GAATGC 2 cut(s) 725, 1106
MvaI CCWGG 5 cut(s) 180, 262, 533, 1003, 1173
MwoI GCNNNNNNNGC 3 cut(s) 998, 1073, 1229
NaeI GCCGGC 1 cut(s) 924
NciI CCSGG 1 cut(s) 1133
NcoI CCATGG 1 cut(s) 1358
NdeI CATATG 1 cut(s) 751
NdeII GATC 5 cut(s) 367, 431, 435, 651, 728
NgoMIV GCCGGC 1 cut(s) 922
NlaIII CATG 5 cut(s) 294, 773, 1262, 1312, 1362
NlaIV GGNNCC 4 cut(s) 313, 379, 813, 1351
NmuCI GTSAC 6 cut(s) 112, 199, 623, 698, 1039, 1455
NsiI ATGCAT 1 cut(s) 1314
NspI RCATGY 1 cut(s) 773
PceI AGGCCT 2 cut(s) 536, 1006
PciI ACATGT 1 cut(s) 769
PctI GAATGC 2 cut(s) 725, 1106
PdiI GCCGGC 1 cut(s) 924
PfeI GAWTC 4 cut(s) 459, 846, 982, 1543
PflFI GACNNNGTC 1 cut(s) 197
PkrI GCNGC 4 cut(s) 418, 527, 1000, 1098
PleI GAGTC 5 cut(s) 118, 548, 749, 1184, 1357
PpsI GAGTC 5 cut(s) 118, 548, 749, 1184, 1357
PscI ACATGT 1 cut(s) 769
PsiI TTATAA 1 cut(s) 593
Psp6I CCWGG 5 cut(s) 178, 260, 531, 1001, 1171
PspEI GGTNACC 1 cut(s) 623
PspGI CCWGG 5 cut(s) 178, 260, 531, 1001, 1171
PspN4I GGNNCC 4 cut(s) 313, 379, 813, 1351
PspPI GGNCC 5 cut(s) 182, 241, 914, 929, 1355
PsrI GAACNNNNNNTAC 2 cut(s) 326, 358
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 2 cut(s) 651, 728
PsyI GACNNNGTC 1 cut(s) 197
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
RseI CAYNNNNRTG 1 cut(s) 1317
SaqAI TTAA 6 cut(s) 231, 257, 273, 803, 1128, 1187
SatI GCNGC 4 cut(s) 417, 526, 999, 1097
Sau3AI GATC 5 cut(s) 367, 431, 435, 651, 728
Sau96I GGNCC 5 cut(s) 182, 241, 914, 929, 1355
SchI GAGTC 5 cut(s) 118, 548, 749, 1185, 1357
ScrFI CCNGG 6 cut(s) 180, 262, 533, 1003, 1133, 1173
SfaNI GCATC 2 cut(s) 845, 1232
SfcI CTRYAG 1 cut(s) 1608
SinI GGWCC 4 cut(s) 241, 914, 929, 1355
SmiMI CAYNNNNRTG 1 cut(s) 1317
SmlI CTYRAG 1 cut(s) 1483
SmoI CTYRAG 1 cut(s) 1483
SpeI ACTAGT 1 cut(s) 605
Sse9I AATT 9 cut(s) 156, 173, 228, 316, 404, 738, 1056, 1069, 1272
SseBI AGGCCT 2 cut(s) 536, 1006
SsiI CCGC 2 cut(s) 329, 1203
SspMI CTAG 5 cut(s) 186, 606, 794, 852, 1407
StuI AGGCCT 2 cut(s) 536, 1006
StyD4I CCNGG 6 cut(s) 178, 260, 531, 1001, 1131, 1171
StyI CCWWGG 4 cut(s) 363, 628, 1358, 1468
TaaI ACNGT 2 cut(s) 112, 1524
TaiI ACGT 1 cut(s) 198
TaqI TCGA 2 cut(s) 434, 457
TasI AATT 9 cut(s) 156, 173, 228, 316, 404, 738, 1056, 1069, 1272
TfiI GAWTC 4 cut(s) 459, 846, 982, 1543
Tru1I TTAA 6 cut(s) 231, 257, 273, 803, 1128, 1187
Tru9I TTAA 6 cut(s) 231, 257, 273, 803, 1128, 1187
TscAI CASTG 4 cut(s) 57, 625, 882, 953
TseFI GTSAC 6 cut(s) 112, 199, 623, 698, 1039, 1455
TseI GCWGC 4 cut(s) 416, 525, 998, 1096
Tsp45I GTSAC 6 cut(s) 112, 199, 623, 698, 1039, 1455
TspDTI ATGAA 3 cut(s) 1011, 1255, 1305
TspGWI ACGGA 1 cut(s) 895
TspRI CASTG 4 cut(s) 57, 625, 882, 953
Tth111I GACNNNGTC 1 cut(s) 197
VpaK11BI GGWCC 4 cut(s) 241, 914, 929, 1355
XceI RCATGY 1 cut(s) 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 35
XmiI GTMKAC 1 cut(s) 444
XspI CTAG 5 cut(s) 186, 606, 794, 852, 1407
Zsp2I ATGCAT 1 cut(s) 1314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.