FvH4_1g00540
ERF Family

Rhodanese Cell cycle control phosphatase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007175
Physical Location & Seq
Forward (+)
293052 .. 298496
5445 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00540.t2

Sequence Viewer

Length: 1968 bp
ATGTGGATGTTACAGGTTTTGTTAAATGGAGGTTTGCATCCAATTTCTCCTTTCCACAAGGATTTTGAGGCCAGATATGTCGTGGACTATAGGAGGTTGTCAAATGCAATGCATTTCCAAAGAGACTCCGTTAAAGCTCATGCTACAAAGTCCTTGTACTCTAATGTTGTGAAAATCACAGAGCAGTCAATTCCCATGGATTTTGTTAACAGGAGTTTCTGCTCAAATGAATCAGACAATATTAGCTGCAAGTACTCAGATGTGTGGAGTTCTTCTAGAGAGACTGGAGATTACCAGCATTCACTGGGAAGGTTTGAACTGAAATATGTAGAGAATTCTAGCCTATTGACCACCAATGAGGGACTCGTGGAGTTCACTGACCAGTCCACTGAGAATGCTGGTACTTTATATACACAAGTAGAACCGGAAACCATGTCAGCTACTGATTTGACACAAGGAACTTCCACATCTGTATCGGACACAATAGACATGAACAGTGATCAATTGTCCAGCGCGAAACCTAGTATTGAGGATCTTATTGATGGAGTTACAAACTCCTTCAGTGCGTCTGTGGATAAGGGAGAAAACGCTTTGAAAAGCTCACTAGATACAATCTCTTCGTCAATATCCTCAGTTGTTGAAAGGGTTAATGAAGCAGTTGATAATGCTGTTAGCGGAGTTTCTTCGACTGTTGATCAAACAGGACAATTAAGTAGTACTAAACTGACAAATTTTTCAAGTGACTTTAAGGAAGCCACAAGTAAAGGAAGTGTGATTGCTGTTGACCTTTTGAGAAGCACAATTGTTGTGGTGGAGGATACTCTAGCAAATGGGGCTTCCTTCGCTGTTAACTCTTATCAATCTTTCAAGGCCTTTCTTCCTCCTGACATCAGTGATGCACTAAATTTGTCAGAAAAGAGAGCATCAGAGCTTTTGGGGCCTGCCAAGGATGCTTTTCAACAGGTTTATCTTGCTGTCGAGGGGTTGGAGGAAAGTCTTGGCTTGGATCCAAATGATCCGATTATTCCATTTGTTCTATTCCTTGGCACCTCTGCCACTTTATGGGTCATTTATTTGGTGTGGACATACAGCGGTTATTCTGGAGATTTATCTCCTCAATCTACATTGGAGCTCTTGACGGGGAAGGAGAATGCTGTTCTCGTTGATGTCCGACCCGAGGTTCTGAGAGAAAAAGAAGGTATTCCTGATCTAAGACGAGCAGCTCGCTATCGCTATGCAAATGTGACTCTACCTGAGATTGATGCCGCAGTAGGGAAGTTGTTGAAGAATGGAAGAGATCTTGATGACACCTTAACTGCTGCTGTTATTCGGAACTTGAAGATTGTTCAGGACAGGTCTGAGGTCATAATTATGGATGCTGATGGTACTCGTTCCAAAGCAATTGCAAGATCCTTGAGAAAGCTGGGACATAAGAAACCTTACTTGGTTCAAGGTGGCTTTCGATCTTGGGTGAAAGATGGTCTTCGAATTAAAGAACTCAAACCTGAGACAACACTTACCATACTCAATGAGGACGCTGAGGCTATTCTGGAGGAAATCAGTCCTTCACCGCTGCAAGTTCTTGGGTATGGTGTGGGTTTGATAGCGGGACTGTATGCACTACTAGAGTGGGAAAAGACATTACAACTGATTGGCGTTGTTGGCCTTGGTCAGACCATCTATCGGCGGGTGGCATCTTATGAGAATGCTGAAGATTTTAAGCAAGATGTGAGGCTGCTGCTTGTTCCGGCACAAGTTGGAGCTCTGGCATTTTCGTGGGCTGCTGGAAAATTGGAATCAAATGGCATTGGACTACCAACATCTCCTTCATCATCAGATGTCAAAAACCGGGTGTTGCAGGCGGCTGCGAAGCATCAATCTCAACCGTCTGATACCGATGGCATCCAAGATTCATCTCCTGGATCAACAAATCCAGCCAATGAGAATGTAGATCTCTCTGAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

656

Amino Acids

71.3

Weight (kDa)

4.78

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 391 - 507 1.7e-07 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1046
AccB7I CCANNNNNTGG 1 cut(s) 1062
AccII CGCG 1 cut(s) 515
AciI CCGC 7 cut(s) 675, 1092, 1266, 1571, 1607, 1687, 1862
AclWI GGATC 6 cut(s) 540, 1001, 1010, 1014, 1404, 1930
AcsI RAATTY 3 cut(s) 334, 730, 904
AcuI CTGAAG 2 cut(s) 544, 1731
AfaI GTAC 5 cut(s) 158, 254, 403, 718, 1387
AfiI CCNNNNNNNGG 4 cut(s) 1062, 1177, 1272, 1683
AgsI TTSAA 9 cut(s) 317, 595, 641, 738, 868, 959, 1285, 1339, 1451
AjnI CCWGG 1 cut(s) 1918
AjuI GAANNNNNNNTTGG 4 cut(s) 1427, 1459, 1810, 1842
AluBI AGCT 9 cut(s) 137, 246, 440, 600, 931, 1132, 1223, 1423, 1763
AluI AGCT 9 cut(s) 137, 246, 440, 600, 931, 1132, 1223, 1423, 1763
Alw21I GWGCWC 2 cut(s) 1134, 1765
Alw26I GTCTC 3 cut(s) 117, 275, 1502
AlwI GGATC 6 cut(s) 540, 1001, 1010, 1014, 1404, 1930
AlwNI CAGNNNCTG 1 cut(s) 443
Ama87I CYCGRG 1 cut(s) 1175
AoxI GGCC 4 cut(s) 69, 870, 938, 1663
ApeKI GCWGC 8 cut(s) 246, 1220, 1319, 1573, 1735, 1738, 1781, 1865
ApoI RAATTY 3 cut(s) 334, 730, 904
Asp700I GAANNNNTTC 1 cut(s) 1200
AspLEI GCGC 1 cut(s) 515
AspS9I GGNCC 1 cut(s) 938
AsuC2I CCSGG 1 cut(s) 1850
AsuHPI GGTGA 2 cut(s) 1483, 1560
AsuII TTCGAA 1 cut(s) 1486
AvaI CYCGRG 1 cut(s) 1175
BaeI ACNNNNGTAYC 2 cut(s) 456, 489
BamHI GGATCC 1 cut(s) 1006
BanI GGYRCC 1 cut(s) 1046
BanII GRGCYC 2 cut(s) 1134, 1765
BauI CACGAG 1 cut(s) 365
BbsI GAAGAC 1 cut(s) 1475
Bbv12I GWGCWC 2 cut(s) 1134, 1765
BbvCI CCTCAGC 1 cut(s) 1539
BbvI GCAGC 8 cut(s) 233, 1232, 1306, 1560, 1722, 1725, 1768, 1852
BccI CCATC 5 cut(s) 536, 1376, 1472, 1685, 1892
BciT130I CCWGG 1 cut(s) 1920
BciVI GTATCC 1 cut(s) 811
BclI TGATCA 2 cut(s) 499, 694
BcnI CCSGG 1 cut(s) 1850
BcoDI GTCTC 3 cut(s) 117, 275, 1502
BfaI CTAG 6 cut(s) 276, 339, 522, 605, 824, 1625
BfmI CTRYAG 1 cut(s) 88
BfuI GTATCC 1 cut(s) 811
BglII AGATCT 2 cut(s) 1297, 1951
BmcAI AGTACT 2 cut(s) 254, 718
Bme1390I CCNGG 2 cut(s) 1850, 1920
BmeT110I CYCGRG 1 cut(s) 1175
BmgT120I GGNCC 1 cut(s) 938
BmiI GGNNCC 3 cut(s) 939, 1008, 1048
BmrFI CCNGG 2 cut(s) 1850, 1920
BmrI ACTGGG 1 cut(s) 314
BmsI GCATC 9 cut(s) 46, 886, 932, 940, 1252, 1366, 1703, 1882, 1911
BmuI ACTGGG 1 cut(s) 314
BpiI GAAGAC 1 cut(s) 1475
BpmI CTGGAG 3 cut(s) 306, 1122, 1571
Bpu10I CCTNAGC 1 cut(s) 1539
Bpu14I TTCGAA 1 cut(s) 1486
BpuEI CTTGAG 1 cut(s) 1435
BpuMI CCSGG 1 cut(s) 1850
BsaJI CCNNGG 5 cut(s) 195, 945, 1042, 1176, 1666
BsaWI WCCGGW 1 cut(s) 424
Bsc4I CCNNNNNNNGG 4 cut(s) 1062, 1177, 1272, 1683
Bse1I ACTGG 3 cut(s) 289, 309, 382
Bse3DI GCAATG 1 cut(s) 114
BseBI CCWGG 1 cut(s) 1920
BseDI CCNNGG 5 cut(s) 195, 945, 1042, 1176, 1666
BseGI GGATG 5 cut(s) 12, 37, 955, 1381, 1902
BseLI CCNNNNNNNGG 4 cut(s) 1062, 1177, 1272, 1683
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 8 cut(s) 270, 381, 645, 1175, 1245, 1350, 1497, 1530
BseNI ACTGG 3 cut(s) 289, 309, 382
BseRI GAGGAG 1 cut(s) 1104
BseXI GCAGC 8 cut(s) 233, 1232, 1306, 1560, 1722, 1725, 1768, 1852
BseYI CCCAGC 1 cut(s) 1423
Bsh1236I CGCG 1 cut(s) 515
BshFI GGCC 4 cut(s) 71, 872, 940, 1665
BshNI GGYRCC 1 cut(s) 1046
BsiHKAI GWGCWC 2 cut(s) 1134, 1765
BsiHKCI CYCGRG 1 cut(s) 1175
BsiSI CCGG 3 cut(s) 425, 1748, 1849
BslFI GGGAC 3 cut(s) 375, 1440, 1623
BslI CCNNNNNNNGG 4 cut(s) 1062, 1177, 1272, 1683
BsmAI GTCTC 3 cut(s) 117, 275, 1502
BsmFI GGGAC 3 cut(s) 375, 1440, 1623
BsmI GAATGC 4 cut(s) 298, 400, 1156, 1711
BsnI GGCC 4 cut(s) 71, 872, 940, 1665
BsoBI CYCGRG 1 cut(s) 1175
Bsp119I TTCGAA 1 cut(s) 1486
Bsp1286I GDGCHC 2 cut(s) 1134, 1765
Bsp19I CCATGG 1 cut(s) 195
BspACI CCGC 7 cut(s) 675, 1092, 1266, 1571, 1607, 1687, 1862
BspANI GGCC 4 cut(s) 71, 872, 940, 1665
BspCNI CTCAG 8 cut(s) 269, 382, 644, 1176, 1246, 1351, 1498, 1531
BspFNI CGCG 1 cut(s) 515
BspLI GGNNCC 3 cut(s) 939, 1008, 1048
BspPI GGATC 6 cut(s) 540, 1001, 1010, 1014, 1404, 1930
BspT104I TTCGAA 1 cut(s) 1486
BspT107I GGYRCC 1 cut(s) 1046
BsrDI GCAATG 1 cut(s) 114
BsrI ACTGG 3 cut(s) 289, 309, 382
BssECI CCNNGG 5 cut(s) 195, 945, 1042, 1176, 1666
BssSI CACGAG 1 cut(s) 365
BssT1I CCWWGG 4 cut(s) 195, 945, 1042, 1666
Bst2BI CACGAG 1 cut(s) 365
Bst2UI CCWGG 1 cut(s) 1920
Bst4CI ACNGT 4 cut(s) 497, 691, 1614, 1887
Bst6I CTCTTC 2 cut(s) 622, 1288
BstBI TTCGAA 1 cut(s) 1486
BstC8I GCNNGC 3 cut(s) 942, 1225, 1860
BstDEI CTNAG 9 cut(s) 256, 390, 631, 1184, 1211, 1254, 1359, 1506, 1539
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 5 cut(s) 12, 37, 955, 1381, 1902
BstFNI CGCG 1 cut(s) 515
BstHHI GCGC 1 cut(s) 515
BstMAI GTCTC 3 cut(s) 117, 275, 1502
BstMWI GCNNNNNNNGC 5 cut(s) 833, 842, 937, 950, 1662
BstNI CCWGG 1 cut(s) 1920
BstSCI CCNGG 2 cut(s) 1848, 1918
BstSFI CTRYAG 1 cut(s) 88
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 8 cut(s) 233, 1232, 1306, 1560, 1722, 1725, 1768, 1852
BstV2I GAAGAC 1 cut(s) 1475
BstX2I RGATCY 5 cut(s) 532, 1006, 1297, 1409, 1951
BstYI RGATCY 5 cut(s) 532, 1006, 1297, 1409, 1951
BsuI GTATCC 1 cut(s) 811
BsuRI GGCC 4 cut(s) 71, 872, 940, 1665
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 5 cut(s) 12, 37, 955, 1381, 1902
BtsIMutI CAGTG 6 cut(s) 302, 375, 387, 502, 568, 898
Cac8I GCNNGC 3 cut(s) 942, 1225, 1860
CaiI CAGNNNCTG 1 cut(s) 443
CfoI GCGC 1 cut(s) 515
Cfr13I GGNCC 1 cut(s) 938
CseI GACGC 2 cut(s) 555, 1544
Csp6I GTAC 5 cut(s) 157, 253, 402, 717, 1386
CspCI CAANNNNNGTGG 2 cut(s) 789, 824
CviAII CATG 4 cut(s) 140, 196, 433, 490
CviQI GTAC 5 cut(s) 157, 253, 402, 717, 1386
DdeI CTNAG 9 cut(s) 256, 390, 631, 1184, 1211, 1254, 1359, 1506, 1539
Eam1104I CTCTTC 2 cut(s) 622, 1288
EarI CTCTTC 2 cut(s) 622, 1288
Ecl136II GAGCTC 2 cut(s) 1132, 1763
Eco130I CCWWGG 4 cut(s) 195, 945, 1042, 1666
Eco147I AGGCCT 1 cut(s) 872
Eco24I GRGCYC 2 cut(s) 1134, 1765
Eco53kI GAGCTC 2 cut(s) 1132, 1763
Eco57I CTGAAG 2 cut(s) 544, 1731
Eco88I CYCGRG 1 cut(s) 1175
EcoICRI GAGCTC 2 cut(s) 1132, 1763
EcoO109I RGGNCCY 1 cut(s) 938
EcoRI GAATTC 1 cut(s) 334
EcoRII CCWGG 1 cut(s) 1918
EcoT14I CCWWGG 4 cut(s) 195, 945, 1042, 1666
EcoT22I ATGCAT 1 cut(s) 114
EcoT38I GRGCYC 2 cut(s) 1134, 1765
ErhI CCWWGG 4 cut(s) 195, 945, 1042, 1666
FaeI CATG 4 cut(s) 143, 199, 436, 493
FalI AAGNNNNNCTT 2 cut(s) 1467, 1499
FaqI GGGAC 3 cut(s) 375, 1440, 1623
FatI CATG 4 cut(s) 139, 195, 432, 489
FauI CCCGC 2 cut(s) 1600, 1680
FbaI TGATCA 2 cut(s) 499, 694
FokI GGATG 5 cut(s) 19, 24, 962, 1388, 1889
FriOI GRGCYC 2 cut(s) 1134, 1765
FspBI CTAG 6 cut(s) 276, 339, 522, 605, 824, 1625
GlaI GCGC 1 cut(s) 514
GsaI CCCAGC 1 cut(s) 1427
GsuI CTGGAG 3 cut(s) 306, 1122, 1571
HaeIII GGCC 4 cut(s) 71, 872, 940, 1665
HapII CCGG 3 cut(s) 425, 1748, 1849
HgaI GACGC 2 cut(s) 555, 1544
HhaI GCGC 1 cut(s) 515
Hin1II CATG 4 cut(s) 143, 199, 436, 493
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HincII GTYRAC 3 cut(s) 208, 784, 850
HindII GTYRAC 3 cut(s) 208, 784, 850
HinfI GANTC 6 cut(s) 125, 230, 363, 1246, 1796, 1910
HpaI GTTAAC 2 cut(s) 208, 850
HpaII CCGG 3 cut(s) 425, 1748, 1849
HphI GGTGA 2 cut(s) 1483, 1560
Hpy166II GTNNAC 7 cut(s) 85, 208, 375, 387, 784, 850, 1083
Hpy188III TCNNGA 8 cut(s) 276, 884, 1101, 1135, 1205, 1301, 1349, 1550
Hpy8I GTNNAC 7 cut(s) 85, 208, 375, 387, 784, 850, 1083
HpyAV CCTTC 7 cut(s) 303, 568, 850, 1138, 1190, 1575, 1836
HpyCH4III ACNGT 4 cut(s) 497, 691, 1614, 1887
HpyF10VI GCNNNNNNNGC 5 cut(s) 833, 842, 937, 950, 1662
HpyF3I CTNAG 9 cut(s) 256, 390, 631, 1184, 1211, 1254, 1359, 1506, 1539
Hsp92II CATG 4 cut(s) 143, 199, 436, 493
HspAI GCGC 1 cut(s) 513
Ksp22I TGATCA 2 cut(s) 499, 694
KspAI GTTAAC 2 cut(s) 208, 850
LmnI GCTCC 2 cut(s) 1129, 1760
Lsp1109I GCAGC 8 cut(s) 233, 1232, 1306, 1560, 1722, 1725, 1768, 1852
LweI GCATC 9 cut(s) 46, 886, 932, 940, 1252, 1366, 1703, 1882, 1911
MaeI CTAG 6 cut(s) 276, 339, 522, 605, 824, 1625
MaeIII GTNAC 4 cut(s) 9, 547, 740, 1243
MboII GAAGA 9 cut(s) 264, 609, 675, 869, 1297, 1305, 1351, 1475, 1724
MfeI CAATTG 3 cut(s) 503, 801, 1401
MflI RGATCY 5 cut(s) 532, 1006, 1297, 1409, 1951
MhlI GDGCHC 2 cut(s) 1134, 1765
MlyI GAGTC 3 cut(s) 119, 357, 1240
MmeI TCCRAC 3 cut(s) 966, 1195, 1738
Mph1103I ATGCAT 1 cut(s) 114
MroXI GAANNNNTTC 1 cut(s) 1200
MslI CAYNNNNRTG 2 cut(s) 1370, 1774
MspA1I CMGCKG 2 cut(s) 1092, 1573
MspI CCGG 3 cut(s) 425, 1748, 1849
MspR9I CCNGG 2 cut(s) 1850, 1920
MunI CAATTG 3 cut(s) 503, 801, 1401
Mva1269I GAATGC 4 cut(s) 298, 400, 1156, 1711
MvaI CCWGG 1 cut(s) 1920
MvnI CGCG 1 cut(s) 515
MwoI GCNNNNNNNGC 5 cut(s) 833, 842, 937, 950, 1662
NciI CCSGG 1 cut(s) 1850
NcoI CCATGG 1 cut(s) 195
NlaIII CATG 4 cut(s) 143, 199, 436, 493
NlaIV GGNNCC 3 cut(s) 939, 1008, 1048
NmuCI GTSAC 2 cut(s) 740, 1243
NsiI ATGCAT 1 cut(s) 114
NspV TTCGAA 1 cut(s) 1486
PceI AGGCCT 1 cut(s) 872
PctI GAATGC 4 cut(s) 298, 400, 1156, 1711
PdmI GAANNNNTTC 1 cut(s) 1200
PfeI GAWTC 3 cut(s) 230, 1796, 1910
PflMI CCANNNNNTGG 1 cut(s) 1062
PfoI TCCNGGA 1 cut(s) 1918
PleI GAGTC 3 cut(s) 119, 357, 1240
PpsI GAGTC 3 cut(s) 119, 357, 1240
Psp124BI GAGCTC 2 cut(s) 1134, 1765
Psp6I CCWGG 1 cut(s) 1918
PspFI CCCAGC 1 cut(s) 1423
PspGI CCWGG 1 cut(s) 1918
PspN4I GGNNCC 3 cut(s) 939, 1008, 1048
PspPI GGNCC 1 cut(s) 938
PstNI CAGNNNCTG 1 cut(s) 443
PsuI RGATCY 5 cut(s) 532, 1006, 1297, 1409, 1951
RsaI GTAC 5 cut(s) 158, 254, 403, 718, 1387
RsaNI GTAC 5 cut(s) 157, 253, 402, 717, 1386
RseI CAYNNNNRTG 2 cut(s) 1370, 1774
SacI GAGCTC 2 cut(s) 1134, 1765
Sau96I GGNCC 1 cut(s) 938
ScaI AGTACT 2 cut(s) 254, 718
SchI GAGTC 3 cut(s) 119, 357, 1240
ScrFI CCNGG 2 cut(s) 1850, 1920
SduI GDGCHC 2 cut(s) 1134, 1765
SfaNI GCATC 9 cut(s) 46, 886, 932, 940, 1252, 1366, 1703, 1882, 1911
SfcI CTRYAG 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 1486
SmiMI CAYNNNNRTG 2 cut(s) 1370, 1774
SmlI CTYRAG 1 cut(s) 1414
SmoI CTYRAG 1 cut(s) 1414
SseBI AGGCCT 1 cut(s) 872
SsiI CCGC 7 cut(s) 675, 1092, 1266, 1571, 1607, 1687, 1862
SspI AATATT 1 cut(s) 241
SspMI CTAG 6 cut(s) 276, 339, 522, 605, 824, 1625
SstI GAGCTC 2 cut(s) 1134, 1765
StuI AGGCCT 1 cut(s) 872
StyD4I CCNGG 2 cut(s) 1848, 1918
StyI CCWWGG 4 cut(s) 195, 945, 1042, 1666
TaaI ACNGT 4 cut(s) 497, 691, 1614, 1887
TaqI TCGA 4 cut(s) 686, 978, 1462, 1486
TatI WGTACW 3 cut(s) 156, 252, 716
TauI GCSGC 2 cut(s) 1268, 1865
TfiI GAWTC 3 cut(s) 230, 1796, 1910
TscAI CASTG 6 cut(s) 309, 382, 394, 502, 568, 898
TseFI GTSAC 2 cut(s) 740, 1243
TseI GCWGC 8 cut(s) 246, 1220, 1319, 1573, 1735, 1738, 1781, 1865
Tsp45I GTSAC 2 cut(s) 740, 1243
TspDTI ATGAA 5 cut(s) 243, 506, 666, 1818, 1902
TspGWI ACGGA 1 cut(s) 118
TspRI CASTG 6 cut(s) 309, 382, 394, 502, 568, 898
Van91I CCANNNNNTGG 1 cut(s) 1062
XapI RAATTY 3 cut(s) 334, 730, 904
XbaI TCTAGA 1 cut(s) 275
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XmnI GAANNNNTTC 1 cut(s) 1200
XspI CTAG 6 cut(s) 276, 339, 522, 605, 824, 1625
ZrmI AGTACT 2 cut(s) 254, 718
Zsp2I ATGCAT 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.