Rh2DG008200
ERF Family

Rhodanese Cell cycle control phosphatase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007175
Physical Location & Seq
Forward (+)
481288 .. 486355
5068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG008200.1

Sequence Viewer

Length: 1983 bp
ATGCTACCTGTTTGCTCTGCCACCTCAAACTGCTGCTCCTCTCATTCTCAGGTTTTGTTAAATGGAGGTTTGCATCCAATTTCCTCGTTCGAGGCCAGATATGTTGTGGATTATAGGGGCTTGTCAAATGCAATGCATTTCCAGAGAGACTCCGTCAAAGCACATGCTACAAAGTCTTTCTACCCTAATGTTGTGACAAGCACAGAACAGTCGGTTCCCATGGATTTTGTTGACGGGAGTTCCTGCTCTAACAAATCAGACTATCTTAATTGCAAGTATTCAGATATGTGGACTTCTTCTGGAGATAAACAGCATTCGTTGGGAACGTTTGAACTGAAATATGTAGAAAGCTCTAGCCTATTGACCACCAATGAGGGACTCGAGGAGTTTACTGACCAGTCCACTGAGAATGCCGGTACTTTATTCAGACCAGTAGAGCCGGAAACCATATCAGCTACTGATTTGACACAAGGATATTCCACCTCTGTATCGGACACCCTAGACATGAATAATGATCAATTGTCCAGCGTGAAACCTAGTATTGAGGATCTTATTGATGGGGTTAGCAAGTCTTTCAGTGCTTCAGTAGATAAGGGAGAAAGTGCTGTGAAAAGCTCACTAGATACAATCACTTCGTCAATATCATCTGTTGTTGAAAGGGTTAATGCAGCAGTGGATAATACTGTTGGCGGAATTTCTTCAACCGTTGATCAAACGGGACAATTAGGTGGTACTAAACTGACAAACTTTTCAAGTGACTTTAAGGAAGCCACAAGTAAAGGAAGTGTGATTGCTGTTGACCTTTTGAGACGCACAATTGTTGTGGTGGAGGATTCTCTAGCAAATGGGGTTTCCTTCGCTGTTAATTCTTACCAATCTTCCAAGGCCTTTCTTCCTCCTGACATCAGTGATGCACTAAATTTGTCTGAAAAGAGAGCAGCAGAGTTTTTGGGGCCTGCCAATGCTGCTTTTCAACAGGTTTACATTGCTATCGAGGGGTTGGAGGAAAGTCTTGGCTTGGATCCAAATGATCCAATTATTCCATTTGTTCTATTCCTTGGCACCTCTGCCACTTTATGGGTCATTTATTCGGTGTGGACATACAGTGGTTATGCTGGAGATTTATCTCCTCAATCTACATTGGAGCTCTTGAAAGGGAAGGAGAAGGCTGTACTCATTGATGTCCGACCCGAGGTTCTGAGAGAAAAAGAAGGTATTCCTGATCTAAGACGAGGAGCTCGCTATCGCTATGCCAGTGTGACTCTACCTGAGGTTGATGCCTCAGTAGCAAAGTTGTTGAAGAATGGAAGAGATCTTGATGACACCTTAACTGCTGCTGTTGTTCGGAACTTGAAGATTGTTCAGGACAGGTCCAAGGTCATAATTATGGATGCTGATGGTACTCGTTCCAAAGCAATTGCAAGATCCTTGAGAAAGCTGGGGCTTAAGATGCCTTACTTGCTTCAAGGTGGCTTTCGATCTTGGGTGAAAGATGGTCTTCGAATTAAAGAACTTAAACCTGAGACAACACTTACCATACTCAATGAGGATGCTGAGGCTATTCTGGAGGAAATCAGTCCTTCTCCGCTTCAAGTTCTGGGGTATGGTGTGGGTTTGATTGCGGGAGTGTATGCACTACTAGAGTGGGAAAAGACATTACAACTGATTGGAGTTGTTGGCCTTGGTCAGACCATTTATCGGCGGGTGGCATCTTATGAGAACGCTGAAGATTTTAAGCAAGATGTGAGGCTGCTGCTTGTTCCGGCACAAGTTGGAGCTCAGGCATTTTCGTGGGCTGCTGGAAAATTGGAATCAAATGGCATCGGACTACCAACATCTCCTTCATCCTCAGCTGTCAAAAACCGGGTGTTGCAGGCGGCTGCAAAGCATCAATCTCAACCGTCTGATACCGATGGCATCCAAGACTCATCTCCTGGATCAACAAATCCAGTCAATGAGAATGTAGATCTCTCTGAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

660

Amino Acids

71.1

Weight (kDa)

4.87

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 381 - 497 7.9e-07 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1061
AccB7I CCANNNNNTGG 1 cut(s) 1077
AciI CCGC 5 cut(s) 690, 1586, 1622, 1702, 1877
AclI AACGTT 1 cut(s) 326
AclWI GGATC 6 cut(s) 555, 1016, 1025, 1029, 1419, 1945
AcsI RAATTY 2 cut(s) 693, 919
AcuI CTGAAG 2 cut(s) 567, 1746
AfaI GTAC 4 cut(s) 418, 733, 1173, 1402
AfiI CCNNNNNNNGG 3 cut(s) 1077, 1192, 1698
AflII CTTAAG 1 cut(s) 1445
AjnI CCWGG 1 cut(s) 1933
AjuI GAANNNNNNNTTGG 2 cut(s) 1825, 1857
AluBI AGCT 8 cut(s) 351, 455, 615, 1147, 1238, 1438, 1778, 1853
AluI AGCT 8 cut(s) 351, 455, 615, 1147, 1238, 1438, 1778, 1853
Alw21I GWGCWC 3 cut(s) 1149, 1240, 1780
Alw26I GTCTC 3 cut(s) 141, 802, 1517
AlwI GGATC 6 cut(s) 555, 1016, 1025, 1029, 1419, 1945
AlwNI CAGNNNCTG 1 cut(s) 458
Ama87I CYCGRG 2 cut(s) 380, 1190
AoxI GGCC 4 cut(s) 93, 885, 953, 1678
ApeKI GCWGC 9 cut(s) 33, 668, 938, 965, 1334, 1750, 1753, 1796, 1880
ApoI RAATTY 2 cut(s) 693, 919
Asp700I GAANNNNTTC 2 cut(s) 697, 1215
AspS9I GGNCC 2 cut(s) 953, 1371
AsuC2I CCSGG 1 cut(s) 1865
AsuHPI GGTGA 1 cut(s) 1498
AsuII TTCGAA 1 cut(s) 1501
AvaI CYCGRG 2 cut(s) 380, 1190
AvaII GGWCC 1 cut(s) 1371
AxyI CCTNAGG 1 cut(s) 1269
BaeI ACNNNNGTAYC 2 cut(s) 471, 504
BamHI GGATCC 1 cut(s) 1021
BanI GGYRCC 1 cut(s) 1061
BanII GRGCYC 3 cut(s) 1149, 1240, 1780
BbsI GAAGAC 1 cut(s) 1490
Bbv12I GWGCWC 3 cut(s) 1149, 1240, 1780
BbvCI CCTCAGC 2 cut(s) 1554, 1849
BbvI GCAGC 9 cut(s) 20, 680, 950, 952, 1321, 1737, 1740, 1783, 1867
BccI CCATC 4 cut(s) 551, 1391, 1487, 1907
BciT130I CCWGG 1 cut(s) 1935
BclI TGATCA 2 cut(s) 514, 709
BcnI CCSGG 1 cut(s) 1865
BcoDI GTCTC 3 cut(s) 141, 802, 1517
BfaI CTAG 6 cut(s) 354, 500, 537, 620, 839, 1640
BfrI CTTAAG 1 cut(s) 1445
BglII AGATCT 2 cut(s) 1312, 1966
Bme1390I CCNGG 2 cut(s) 1865, 1935
Bme18I GGWCC 1 cut(s) 1371
BmeT110I CYCGRG 2 cut(s) 380, 1190
BmgT120I GGNCC 2 cut(s) 953, 1371
BmiI GGNNCC 4 cut(s) 216, 954, 1023, 1063
BmrFI CCNGG 2 cut(s) 1865, 1935
BpiI GAAGAC 1 cut(s) 1490
BpmI CTGGAG 3 cut(s) 321, 1137, 1586
Bpu10I CCTNAGC 3 cut(s) 1554, 1779, 1849
Bpu14I TTCGAA 1 cut(s) 1501
BpuEI CTTGAG 1 cut(s) 1450
BpuMI CCSGG 1 cut(s) 1865
BsaJI CCNNGG 6 cut(s) 219, 882, 1057, 1191, 1374, 1681
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 3 cut(s) 1077, 1192, 1698
Bse118I RCCGGY 1 cut(s) 413
Bse1I ACTGG 4 cut(s) 397, 431, 1254, 1949
Bse21I CCTNAGG 1 cut(s) 1269
Bse3DI GCAATG 2 cut(s) 138, 984
BseBI CCWGG 1 cut(s) 1935
BseDI CCNNGG 6 cut(s) 219, 882, 1057, 1191, 1374, 1681
BseGI GGATG 5 cut(s) 73, 1396, 1555, 1844, 1917
BseLI CCNNNNNNNGG 3 cut(s) 1077, 1192, 1698
BseMI GCAATG 2 cut(s) 138, 984
BseMII CTCAG 9 cut(s) 62, 396, 1190, 1260, 1296, 1512, 1545, 1793, 1863
BseNI ACTGG 4 cut(s) 397, 431, 1254, 1949
BseRI GAGGAG 4 cut(s) 28, 398, 1119, 1248
BseXI GCAGC 9 cut(s) 20, 680, 950, 952, 1321, 1737, 1740, 1783, 1867
BseYI CCCAGC 1 cut(s) 1438
BshFI GGCC 4 cut(s) 95, 887, 955, 1680
BshNI GGYRCC 1 cut(s) 1061
BsiHKAI GWGCWC 3 cut(s) 1149, 1240, 1780
BsiHKCI CYCGRG 2 cut(s) 380, 1190
BsiSI CCGG 4 cut(s) 414, 440, 1763, 1864
BslFI GGGAC 2 cut(s) 390, 732
BslI CCNNNNNNNGG 3 cut(s) 1077, 1192, 1698
BsmAI GTCTC 3 cut(s) 141, 802, 1517
BsmBI CGTCTC 1 cut(s) 802
BsmFI GGGAC 2 cut(s) 390, 732
BsmI GAATGC 2 cut(s) 313, 415
BsnI GGCC 4 cut(s) 95, 887, 955, 1680
BsoBI CYCGRG 2 cut(s) 380, 1190
Bsp119I TTCGAA 1 cut(s) 1501
Bsp1286I GDGCHC 3 cut(s) 1149, 1240, 1780
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 5 cut(s) 690, 1586, 1622, 1702, 1877
BspANI GGCC 4 cut(s) 95, 887, 955, 1680
BspCNI CTCAG 9 cut(s) 61, 397, 1191, 1261, 1295, 1513, 1546, 1792, 1862
BspLI GGNNCC 4 cut(s) 216, 954, 1023, 1063
BspPI GGATC 6 cut(s) 555, 1016, 1025, 1029, 1419, 1945
BspT104I TTCGAA 1 cut(s) 1501
BspT107I GGYRCC 1 cut(s) 1061
BspTI CTTAAG 1 cut(s) 1445
BsrDI GCAATG 2 cut(s) 138, 984
BsrFI RCCGGY 1 cut(s) 413
BsrI ACTGG 4 cut(s) 397, 431, 1254, 1949
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 6 cut(s) 219, 882, 1057, 1191, 1374, 1681
BssT1I CCWWGG 5 cut(s) 219, 882, 1057, 1374, 1681
Bst2UI CCWGG 1 cut(s) 1935
Bst4CI ACNGT 5 cut(s) 210, 685, 706, 1106, 1902
Bst6I CTCTTC 1 cut(s) 1303
BstAFI CTTAAG 1 cut(s) 1445
BstBI TTCGAA 1 cut(s) 1501
BstC8I GCNNGC 3 cut(s) 957, 1240, 1875
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 5 cut(s) 73, 1396, 1555, 1844, 1917
BstMAI GTCTC 3 cut(s) 141, 802, 1517
BstMWI GCNNNNNNNGC 4 cut(s) 965, 1286, 1450, 1459
BstNI CCWGG 1 cut(s) 1935
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 2 cut(s) 1863, 1933
BstV1I GCAGC 9 cut(s) 20, 680, 950, 952, 1321, 1737, 1740, 1783, 1867
BstV2I GAAGAC 1 cut(s) 1490
BstX2I RGATCY 5 cut(s) 547, 1021, 1312, 1424, 1966
BstYI RGATCY 5 cut(s) 547, 1021, 1312, 1424, 1966
Bsu36I CCTNAGG 1 cut(s) 1269
BsuRI GGCC 4 cut(s) 95, 887, 955, 1680
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 5 cut(s) 73, 1396, 1555, 1844, 1917
BtsI GCAGTG 1 cut(s) 678
BtsIMutI CAGTG 6 cut(s) 402, 583, 678, 913, 1111, 1261
Cac8I GCNNGC 3 cut(s) 957, 1240, 1875
CaiI CAGNNNCTG 1 cut(s) 458
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 2 cut(s) 953, 1371
CseI GACGC 1 cut(s) 819
Csp6I GTAC 4 cut(s) 417, 732, 1172, 1401
CspCI CAANNNNNGTGG 2 cut(s) 804, 839
CviAII CATG 3 cut(s) 164, 220, 505
CviQI GTAC 4 cut(s) 417, 732, 1172, 1401
Eam1104I CTCTTC 1 cut(s) 1303
EarI CTCTTC 1 cut(s) 1303
EciI GGCGGA 1 cut(s) 705
Ecl136II GAGCTC 3 cut(s) 1147, 1238, 1778
Eco130I CCWWGG 5 cut(s) 219, 882, 1057, 1374, 1681
Eco147I AGGCCT 1 cut(s) 887
Eco24I GRGCYC 3 cut(s) 1149, 1240, 1780
Eco47I GGWCC 1 cut(s) 1371
Eco53kI GAGCTC 3 cut(s) 1147, 1238, 1778
Eco57I CTGAAG 2 cut(s) 567, 1746
Eco81I CCTNAGG 1 cut(s) 1269
Eco88I CYCGRG 2 cut(s) 380, 1190
EcoICRI GAGCTC 3 cut(s) 1147, 1238, 1778
EcoO109I RGGNCCY 1 cut(s) 953
EcoRII CCWGG 1 cut(s) 1933
EcoT14I CCWWGG 5 cut(s) 219, 882, 1057, 1374, 1681
EcoT22I ATGCAT 1 cut(s) 138
EcoT38I GRGCYC 3 cut(s) 1149, 1240, 1780
ErhI CCWWGG 5 cut(s) 219, 882, 1057, 1374, 1681
Esp3I CGTCTC 1 cut(s) 802
FaeI CATG 3 cut(s) 167, 223, 508
FalI AAGNNNNNCTT 2 cut(s) 1482, 1514
FaqI GGGAC 2 cut(s) 390, 732
FatI CATG 3 cut(s) 163, 219, 504
FauI CCCGC 2 cut(s) 1615, 1695
FbaI TGATCA 2 cut(s) 514, 709
FokI GGATG 5 cut(s) 60, 1403, 1562, 1831, 1904
FriOI GRGCYC 3 cut(s) 1149, 1240, 1780
FspBI CTAG 6 cut(s) 354, 500, 537, 620, 839, 1640
GsaI CCCAGC 1 cut(s) 1442
GsuI CTGGAG 3 cut(s) 321, 1137, 1586
HaeIII GGCC 4 cut(s) 95, 887, 955, 1680
HapII CCGG 4 cut(s) 414, 440, 1763, 1864
HgaI GACGC 1 cut(s) 819
Hin1II CATG 3 cut(s) 167, 223, 508
HincII GTYRAC 2 cut(s) 232, 799
HindII GTYRAC 2 cut(s) 232, 799
HinfI GANTC 6 cut(s) 149, 378, 833, 1261, 1811, 1925
HpaII CCGG 4 cut(s) 414, 440, 1763, 1864
HphI GGTGA 1 cut(s) 1498
Hpy166II GTNNAC 7 cut(s) 232, 291, 390, 402, 799, 982, 1098
Hpy188III TCNNGA 8 cut(s) 142, 300, 899, 1150, 1220, 1316, 1364, 1565
Hpy8I GTNNAC 7 cut(s) 232, 291, 390, 402, 799, 982, 1098
HpyAV CCTTC 6 cut(s) 865, 1153, 1159, 1205, 1590, 1851
HpyCH4III ACNGT 5 cut(s) 210, 685, 706, 1106, 1902
HpyCH4IV ACGT 1 cut(s) 326
HpyF10VI GCNNNNNNNGC 4 cut(s) 965, 1286, 1450, 1459
HpySE526I ACGT 1 cut(s) 326
Hsp92II CATG 3 cut(s) 167, 223, 508
Ksp22I TGATCA 2 cut(s) 514, 709
LmnI GCTCC 4 cut(s) 41, 1144, 1235, 1775
Lsp1109I GCAGC 9 cut(s) 20, 680, 950, 952, 1321, 1737, 1740, 1783, 1867
MaeI CTAG 6 cut(s) 354, 500, 537, 620, 839, 1640
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 3 cut(s) 193, 755, 1258
MboII GAAGA 9 cut(s) 288, 690, 870, 884, 1312, 1320, 1366, 1490, 1739
MfeI CAATTG 3 cut(s) 518, 816, 1416
MflI RGATCY 5 cut(s) 547, 1021, 1312, 1424, 1966
MhlI GDGCHC 3 cut(s) 1149, 1240, 1780
MlyI GAGTC 4 cut(s) 143, 372, 1255, 1919
MmeI TCCRAC 3 cut(s) 981, 1210, 1753
Mph1103I ATGCAT 1 cut(s) 138
MroXI GAANNNNTTC 2 cut(s) 697, 1215
MslI CAYNNNNRTG 2 cut(s) 1385, 1789
MspA1I CMGCKG 1 cut(s) 1853
MspCI CTTAAG 1 cut(s) 1445
MspI CCGG 4 cut(s) 414, 440, 1763, 1864
MspR9I CCNGG 2 cut(s) 1865, 1935
MunI CAATTG 3 cut(s) 518, 816, 1416
Mva1269I GAATGC 2 cut(s) 313, 415
MvaI CCWGG 1 cut(s) 1935
MwoI GCNNNNNNNGC 4 cut(s) 965, 1286, 1450, 1459
NciI CCSGG 1 cut(s) 1865
NcoI CCATGG 1 cut(s) 219
NlaIII CATG 3 cut(s) 167, 223, 508
NlaIV GGNNCC 4 cut(s) 216, 954, 1023, 1063
NmuCI GTSAC 3 cut(s) 193, 755, 1258
NsiI ATGCAT 1 cut(s) 138
NspI RCATGY 1 cut(s) 167
NspV TTCGAA 1 cut(s) 1501
PaeR7I CTCGAG 1 cut(s) 380
PceI AGGCCT 1 cut(s) 887
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PctI GAATGC 2 cut(s) 313, 415
PdmI GAANNNNTTC 2 cut(s) 697, 1215
PfeI GAWTC 2 cut(s) 833, 1811
PflFI GACNNNGTC 1 cut(s) 152
PflMI CCANNNNNTGG 1 cut(s) 1077
PfoI TCCNGGA 1 cut(s) 1933
PleI GAGTC 4 cut(s) 143, 372, 1255, 1919
PpsI GAGTC 4 cut(s) 143, 372, 1255, 1919
Psp124BI GAGCTC 3 cut(s) 1149, 1240, 1780
Psp1406I AACGTT 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 1933
PspFI CCCAGC 1 cut(s) 1438
PspGI CCWGG 1 cut(s) 1933
PspN4I GGNNCC 4 cut(s) 216, 954, 1023, 1063
PspPI GGNCC 2 cut(s) 953, 1371
PspXI VCTCGAGB 1 cut(s) 380
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 5 cut(s) 547, 1021, 1312, 1424, 1966
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 1853
RsaI GTAC 4 cut(s) 418, 733, 1173, 1402
RsaNI GTAC 4 cut(s) 417, 732, 1172, 1401
RseI CAYNNNNRTG 2 cut(s) 1385, 1789
SacI GAGCTC 3 cut(s) 1149, 1240, 1780
Sau96I GGNCC 2 cut(s) 953, 1371
SchI GAGTC 4 cut(s) 143, 372, 1255, 1919
ScrFI CCNGG 2 cut(s) 1865, 1935
SduI GDGCHC 3 cut(s) 1149, 1240, 1780
Sfr274I CTCGAG 1 cut(s) 380
SfuI TTCGAA 1 cut(s) 1501
SinI GGWCC 1 cut(s) 1371
SlaI CTCGAG 1 cut(s) 380
SmiMI CAYNNNNRTG 2 cut(s) 1385, 1789
SmlI CTYRAG 3 cut(s) 380, 1429, 1445
SmoI CTYRAG 3 cut(s) 380, 1429, 1445
SseBI AGGCCT 1 cut(s) 887
SsiI CCGC 5 cut(s) 690, 1586, 1622, 1702, 1877
SspMI CTAG 6 cut(s) 354, 500, 537, 620, 839, 1640
SstI GAGCTC 3 cut(s) 1149, 1240, 1780
StuI AGGCCT 1 cut(s) 887
StyD4I CCNGG 2 cut(s) 1863, 1933
StyI CCWWGG 5 cut(s) 219, 882, 1057, 1374, 1681
TaaI ACNGT 5 cut(s) 210, 685, 706, 1106, 1902
TaiI ACGT 1 cut(s) 329
TaqI TCGA 5 cut(s) 90, 381, 993, 1477, 1501
TatI WGTACW 1 cut(s) 1171
TauI GCSGC 1 cut(s) 1880
TfiI GAWTC 2 cut(s) 833, 1811
TscAI CASTG 6 cut(s) 409, 583, 678, 913, 1111, 1261
TseFI GTSAC 3 cut(s) 193, 755, 1258
TseI GCWGC 9 cut(s) 33, 668, 938, 965, 1334, 1750, 1753, 1796, 1880
Tsp45I GTSAC 3 cut(s) 193, 755, 1258
TspDTI ATGAA 2 cut(s) 521, 1833
TspGWI ACGGA 1 cut(s) 142
TspRI CASTG 6 cut(s) 409, 583, 678, 913, 1111, 1261
Tth111I GACNNNGTC 1 cut(s) 152
Van91I CCANNNNNTGG 1 cut(s) 1077
Vha464I CTTAAG 1 cut(s) 1445
VpaK11BI GGWCC 1 cut(s) 1371
XapI RAATTY 2 cut(s) 693, 919
XceI RCATGY 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 380
XmnI GAANNNNTTC 2 cut(s) 697, 1215
XspI CTAG 6 cut(s) 354, 500, 537, 620, 839, 1640
Zsp2I ATGCAT 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.