FvH4_1g09021

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0021004
Physical Location & Seq
Forward (+)
4773485 .. 4774707
1223 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09021.t1

Sequence Viewer

Length: 975 bp
ATGGCAGAAACAGAGGTACCCGAAGATGTTGTTATCTTCAACATCCTACCTAGGCTTCCCATAAAAACCCTAATCCGATTCACCTCCGTTTCGAAACGTTGGCGTTTCCTCATACTATCCGACCGCAACTTCGCCAGAACCCAACTTAGGGTTTCTCGTGAGCACCGCCACAGACTCCTCCTCGTCACCCGCACAGAGTACTCTCCGTCACCAACTTATAAGCTCGACGTCCTAAACTTGCGACACGTGCCGTCGTCGTCCGCGGCTCGAGATTTGAATTTGCCGAAAACATTGCTTTGTCCACTTGGTTCATTCAACGGCTTGGTTTTTATCGTGGACGATTGTGAGAACCTCTGTGTTTTGAACCCGGCCACCGGATTTTTAAAGCAGTTTCCCGGCCCAGGTTTCCCGATCGACGAATGGCTACTCTATTGCGGCGTTGGCTATTTGCCGGCCGCCGACGACTACAAAGTCGCCGTGGCGGTTAACTGGGAAAGAGGGGTTCATGTTGTGATTTTCTCTCTGCTAGATCGAAACTGGAAAACGATTGAAACCCCCGGCTGTGTTCATTTTCAGGGAACTATGGGGACTGTTTTGAACGAGGCTCTGCATTGGGTAGACAAGCTTGATGTGACGGGGATTCTAGCGTTTGATTTGGAGAAGGAGGAGTTCAGGATAATGAGTGTGCCTGATTTAGAATTAGATGACTCTGTTTATTACGTTGGGGTTTATGGTGGAATATCTCTGTGTTTGTTGCGGTACTCCGACAGTGATAGAATCGATGACAGTATTGATTTCTGGGTTCTGACAGAATATGGGGTGCCAGATTCGTGGACAAAGTTGTTTATTTTGAAAATGGAGATTTTTCCTAACCTTACTACGAATAGGACTTGGGATGCACGATACTTGGTTATGGAGAAGAGTAAAGCTGCTGTGGTGGAGGTGCCGGGATCGGAAAGGAGACTGCGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

325

Amino Acids

37.08

Weight (kDa)

5.61

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 86 - 287 6.3e-11 F-box associated beta propeller domain
FBA_1 PF07734 106 - 313 1.1e-12 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021004)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09021
rosa_laevigata RLG00000016567
rosa_multiflora Rmu_sc0010847.1_g000002 Rmu_ssc0000183.1_g000047
rosa_roxburghii Rroxscaffold_2G00146340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 219
AasI GACNNNNNNGTC 1 cut(s) 470
AatII GACGTC 1 cut(s) 231
Acc65I GGTACC 1 cut(s) 16
AccB1I GGYRCC 3 cut(s) 16, 820, 943
AccI GTMKAC 1 cut(s) 618
AccII CGCG 1 cut(s) 263
AciI CCGC 9 cut(s) 124, 166, 190, 261, 263, 435, 456, 482, 757
AclI AACGTT 1 cut(s) 97
AclWI GGATC 1 cut(s) 958
AcoI YGGCCR 2 cut(s) 369, 453
AcsI RAATTY 1 cut(s) 277
AcvI CACGTG 1 cut(s) 247
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 3 cut(s) 18, 200, 761
AfiI CCNNNNNNNGG 4 cut(s) 147, 148, 374, 401
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 7 cut(s) 40, 277, 316, 364, 551, 598, 853
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 3 cut(s) 223, 625, 929
AluI AGCT 3 cut(s) 223, 625, 929
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 955
AlwI GGATC 1 cut(s) 958
Ama87I CYCGRG 1 cut(s) 267
AoxI GGCC 3 cut(s) 369, 397, 453
ApeKI GCWGC 1 cut(s) 929
ApoI RAATTY 1 cut(s) 277
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
Asp718I GGTACC 1 cut(s) 16
AspA2I CCTAGG 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 398
AsuC2I CCSGG 4 cut(s) 368, 396, 558, 948
AsuHPI GGTGA 3 cut(s) 73, 178, 201
AsuII TTCGAA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 267
AvrII CCTAGG 1 cut(s) 50
BaeI ACNNNNGTAYC 1 cut(s) 33
BanI GGYRCC 3 cut(s) 16, 820, 943
BauI CACGAG 1 cut(s) 156
BbrPI CACGTG 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 165
BbvI GCAGC 1 cut(s) 916
BceAI ACGGC 3 cut(s) 235, 334, 461
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BciT130I CCWGG 1 cut(s) 402
BcnI CCSGG 4 cut(s) 368, 396, 558, 948
BcoDI GTCTC 1 cut(s) 955
BfaI CTAG 3 cut(s) 51, 527, 644
BisI GCNGC 4 cut(s) 264, 436, 456, 930
BlnI CCTAGG 1 cut(s) 50
BlsI GCNGC 4 cut(s) 265, 437, 457, 931
BmcAI AGTACT 1 cut(s) 200
Bme1390I CCNGG 5 cut(s) 368, 396, 402, 558, 948
BmeT110I CYCGRG 1 cut(s) 267
BmgT120I GGNCC 1 cut(s) 398
BmiI GGNNCC 3 cut(s) 18, 822, 945
BmrFI CCNGG 5 cut(s) 368, 396, 402, 558, 948
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 1 cut(s) 886
BmuI ACTGGG 1 cut(s) 499
Bpu14I TTCGAA 1 cut(s) 92
BpuMI CCSGG 4 cut(s) 368, 396, 558, 948
Bsa29I ATCGAT 1 cut(s) 780
BsaAI YACGTR 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 228
BsaJI CCNNGG 5 cut(s) 50, 261, 400, 477, 556
BsaWI WCCGGW 1 cut(s) 374
BsaXI ACNNNNNCTCC 1 cut(s) 952
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 148, 374, 401
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 2 cut(s) 494, 542
Bse3DI GCAATG 1 cut(s) 290
BseBI CCWGG 1 cut(s) 402
BseCI ATCGAT 1 cut(s) 780
BseDI CCNNGG 5 cut(s) 50, 261, 400, 477, 556
BseGI GGATG 2 cut(s) 42, 901
BseLI CCNNNNNNNGG 4 cut(s) 147, 148, 374, 401
BseMI GCAATG 1 cut(s) 290
BseNI ACTGG 2 cut(s) 494, 542
BseRI GAGGAG 3 cut(s) 167, 170, 680
BseX3I CGGCCG 1 cut(s) 453
BseXI GCAGC 1 cut(s) 916
Bsh1236I CGCG 1 cut(s) 263
Bsh1285I CGRYCG 3 cut(s) 124, 414, 456
BshFI GGCC 3 cut(s) 371, 399, 455
BshNI GGYRCC 3 cut(s) 16, 820, 943
BshVI ATCGAT 1 cut(s) 780
BsiEI CGRYCG 3 cut(s) 124, 414, 456
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 6 cut(s) 368, 375, 396, 452, 558, 947
BslFI GGGAC 1 cut(s) 601
BslI CCNNNNNNNGG 4 cut(s) 147, 148, 374, 401
BsmAI GTCTC 1 cut(s) 955
BsmFI GGGAC 1 cut(s) 601
BsnI GGCC 3 cut(s) 371, 399, 455
BsoBI CYCGRG 1 cut(s) 267
Bsp119I TTCGAA 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 165
Bsp143I GATC 3 cut(s) 411, 529, 950
BspACI CCGC 9 cut(s) 124, 166, 190, 261, 263, 435, 456, 482, 757
BspANI GGCC 3 cut(s) 371, 399, 455
BspDI ATCGAT 1 cut(s) 780
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 3 cut(s) 18, 822, 945
BspPI GGATC 1 cut(s) 958
BspT104I TTCGAA 1 cut(s) 92
BspT107I GGYRCC 3 cut(s) 16, 820, 943
BsrDI GCAATG 1 cut(s) 290
BsrFI RCCGGY 1 cut(s) 451
BsrI ACTGG 2 cut(s) 494, 542
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 5 cut(s) 50, 261, 400, 477, 556
BssMI GATC 3 cut(s) 411, 529, 950
BssNI GRCGYC 1 cut(s) 228
BssSI CACGAG 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 50
Bst2BI CACGAG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 3 cut(s) 592, 770, 788
Bst6I CTCTTC 1 cut(s) 914
BstACI GRCGYC 1 cut(s) 228
BstBAI YACGTR 1 cut(s) 247
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 1 cut(s) 146
BstDSI CCRYGG 2 cut(s) 261, 477
BstF5I GGATG 2 cut(s) 42, 901
BstFNI CGCG 1 cut(s) 263
BstKTI GATC 3 cut(s) 414, 532, 953
BstMAI GTCTC 1 cut(s) 955
BstMBI GATC 3 cut(s) 411, 529, 950
BstMCI CGRYCG 3 cut(s) 124, 414, 456
BstMWI GCNNNNNNNGC 2 cut(s) 247, 441
BstNI CCWGG 1 cut(s) 402
BstSCI CCNGG 5 cut(s) 366, 394, 400, 556, 946
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 1 cut(s) 916
BstXI CCANNNNNNTGG 1 cut(s) 831
BstZI CGGCCG 1 cut(s) 453
Bsu15I ATCGAT 1 cut(s) 780
BsuRI GGCC 3 cut(s) 371, 399, 455
BsuTUI ATCGAT 1 cut(s) 780
BtgI CCRYGG 2 cut(s) 261, 477
BtsCI GGATG 2 cut(s) 42, 901
BtsIMutI CAGTG 1 cut(s) 775
Cac8I GCNNGC 1 cut(s) 453
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 1 cut(s) 398
Cfr42I CCGCGG 1 cut(s) 264
ClaI ATCGAT 1 cut(s) 780
Csp6I GTAC 3 cut(s) 17, 199, 760
CviAII CATG 1 cut(s) 506
CviQI GTAC 3 cut(s) 17, 199, 760
DdeI CTNAG 1 cut(s) 146
DpnI GATC 3 cut(s) 413, 531, 952
DpnII GATC 3 cut(s) 411, 529, 950
DraI TTTAAA 1 cut(s) 384
DrdI GACNNNNNNGTC 1 cut(s) 470
DseDI GACNNNNNNGTC 1 cut(s) 470
EaeI YGGCCR 2 cut(s) 369, 453
EagI CGGCCG 1 cut(s) 453
Eam1104I CTCTTC 1 cut(s) 914
EarI CTCTTC 1 cut(s) 914
EclXI CGGCCG 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 50
Eco52I CGGCCG 1 cut(s) 453
Eco72I CACGTG 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 1 cut(s) 509
FaiI YATR 8 cut(s) 62, 113, 219, 507, 584, 732, 816, 914
FaqI GGGAC 1 cut(s) 601
FatI CATG 1 cut(s) 505
FauI CCCGC 1 cut(s) 197
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 4 cut(s) 264, 436, 456, 930
FokI GGATG 2 cut(s) 29, 908
Fsp4HI GCNGC 4 cut(s) 264, 436, 456, 930
FspBI CTAG 3 cut(s) 51, 527, 644
GluI GCNGC 4 cut(s) 264, 436, 456, 930
HaeIII GGCC 3 cut(s) 371, 399, 455
HapII CCGG 6 cut(s) 368, 375, 396, 452, 558, 947
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 1 cut(s) 509
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 6 cut(s) 78, 174, 640, 707, 777, 827
HpaI GTTAAC 1 cut(s) 487
HpaII CCGG 6 cut(s) 368, 375, 396, 452, 558, 947
HphI GGTGA 3 cut(s) 73, 178, 201
Hpy166II GTNNAC 5 cut(s) 302, 337, 487, 619, 834
Hpy188I TCNGA 5 cut(s) 77, 121, 766, 807, 955
Hpy188III TCNNGA 4 cut(s) 158, 269, 409, 673
Hpy8I GTNNAC 5 cut(s) 302, 337, 487, 619, 834
Hpy99I CGWCG 5 cut(s) 230, 256, 259, 419, 464
HpyAV CCTTC 1 cut(s) 655
HpyCH4III ACNGT 3 cut(s) 592, 770, 788
HpyCH4IV ACGT 4 cut(s) 97, 228, 246, 720
HpyCH4V TGCA 2 cut(s) 610, 899
HpyF10VI GCNNNNNNNGC 2 cut(s) 247, 441
HpyF3I CTNAG 1 cut(s) 146
HpySE526I ACGT 4 cut(s) 97, 228, 246, 720
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 1 cut(s) 509
KpnI GGTACC 1 cut(s) 20
KroI GCCGGC 1 cut(s) 451
KroNI GCCGGC 1 cut(s) 453
KspAI GTTAAC 1 cut(s) 487
KspI CCGCGG 1 cut(s) 264
Kzo9I GATC 3 cut(s) 411, 529, 950
Lsp1109I GCAGC 1 cut(s) 916
LweI GCATC 1 cut(s) 886
MaeI CTAG 3 cut(s) 51, 527, 644
MaeII ACGT 4 cut(s) 97, 228, 246, 720
MaeIII GTNAC 3 cut(s) 184, 207, 631
MalI GATC 3 cut(s) 413, 531, 952
MboI GATC 3 cut(s) 411, 529, 950
MboII GAAGA 3 cut(s) 28, 35, 931
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 2 cut(s) 277, 698
MlyI GAGTC 2 cut(s) 168, 701
MmeI TCCRAC 2 cut(s) 144, 789
MroNI GCCGGC 1 cut(s) 451
MseI TTAA 3 cut(s) 383, 486, 973
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 6 cut(s) 368, 375, 396, 452, 558, 947
MspR9I CCNGG 5 cut(s) 368, 396, 402, 558, 948
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 2 cut(s) 247, 441
NaeI GCCGGC 1 cut(s) 453
NciI CCSGG 4 cut(s) 368, 396, 558, 948
NdeII GATC 3 cut(s) 411, 529, 950
NgoMIV GCCGGC 1 cut(s) 451
NlaIII CATG 1 cut(s) 509
NlaIV GGNNCC 3 cut(s) 18, 822, 945
NmuCI GTSAC 3 cut(s) 184, 207, 631
NspV TTCGAA 1 cut(s) 92
PaeR7I CTCGAG 1 cut(s) 267
PdiI GCCGGC 1 cut(s) 453
PfeI GAWTC 4 cut(s) 78, 640, 777, 827
PkrI GCNGC 4 cut(s) 265, 437, 457, 931
Ple19I CGATCG 1 cut(s) 414
PleI GAGTC 2 cut(s) 168, 701
PmaCI CACGTG 1 cut(s) 247
PmlI CACGTG 1 cut(s) 247
PpsI GAGTC 2 cut(s) 168, 701
Ppu21I YACGTR 1 cut(s) 247
PsiI TTATAA 1 cut(s) 219
Psp1406I AACGTT 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 400
PspCI CACGTG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 3 cut(s) 18, 822, 945
PspPI GGNCC 1 cut(s) 398
PvuI CGATCG 1 cut(s) 414
RsaI GTAC 3 cut(s) 18, 200, 761
RsaNI GTAC 3 cut(s) 17, 199, 760
SacII CCGCGG 1 cut(s) 264
SaqAI TTAA 3 cut(s) 383, 486, 973
SatI GCNGC 4 cut(s) 264, 436, 456, 930
Sau3AI GATC 3 cut(s) 411, 529, 950
Sau96I GGNCC 1 cut(s) 398
ScaI AGTACT 1 cut(s) 200
SchI GAGTC 2 cut(s) 168, 701
ScrFI CCNGG 5 cut(s) 368, 396, 402, 558, 948
SduI GDGCHC 1 cut(s) 165
SfaNI GCATC 1 cut(s) 886
Sfr274I CTCGAG 1 cut(s) 267
Sfr303I CCGCGG 1 cut(s) 264
SfuI TTCGAA 1 cut(s) 92
SgrBI CCGCGG 1 cut(s) 264
SlaI CTCGAG 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
Sse9I AATT 2 cut(s) 277, 698
SsiI CCGC 9 cut(s) 124, 166, 190, 261, 263, 435, 456, 482, 757
SspMI CTAG 3 cut(s) 51, 527, 644
StyD4I CCNGG 5 cut(s) 366, 394, 400, 556, 946
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 3 cut(s) 592, 770, 788
TaiI ACGT 4 cut(s) 100, 231, 249, 723
TaqI TCGA 6 cut(s) 92, 225, 268, 414, 532, 780
TasI AATT 2 cut(s) 277, 698
TatI WGTACW 1 cut(s) 198
TauI GCSGC 3 cut(s) 266, 438, 458
TfiI GAWTC 4 cut(s) 78, 640, 777, 827
Tru1I TTAA 3 cut(s) 383, 486, 973
Tru9I TTAA 3 cut(s) 383, 486, 973
TscAI CASTG 1 cut(s) 775
TseFI GTSAC 3 cut(s) 184, 207, 631
TseI GCWGC 1 cut(s) 929
Tsp45I GTSAC 3 cut(s) 184, 207, 631
TspDTI ATGAA 3 cut(s) 300, 494, 557
TspGWI ACGGA 2 cut(s) 76, 195
TspRI CASTG 1 cut(s) 775
XapI RAATTY 1 cut(s) 277
XhoI CTCGAG 1 cut(s) 267
XmaJI CCTAGG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 618
XspI CTAG 3 cut(s) 51, 527, 644
ZraI GACGTC 1 cut(s) 229
ZrmI AGTACT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.