Rroxscaffold_2G00146340

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0021004
Physical Location & Seq
Reverse (-)
84255883 .. 84256723
841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146340.1

Sequence Viewer

Length: 696 bp
ATGAAGGGCCCTCCTCGAATCCCTAGACTTGCACGCCCCATCGTTCGGAGACGAATCCTCGGTTCAAAAATTCAACATCGCTTTCTTGAACTCATACGATCAAATTTGGCTTCTAGAGTTCTAGGTTCATGCAATGGCTTGGTTTTTGTCGTCGTCGTCGATTGTCAAAAGTTTTGTGTGTACGTTGAACCCGTCCACCGGACTGTCGAAGGAATTGGCTGGCCCAGGATTGAATCCCCCGGCAACAGCGACGGCTACGGCGGCATTTGTGTTTATGCTATGGGGAATTCGGCGACTGTTTTGAACGAGACTCTTCATTGGCTAGACGAAAGGGATGCGATAGTGGCTTTTGATTTGGAGAAGGAAGAGTTAAGGAAAATGAGCCTACCTCATTTCCTCATAAATTACTCGACTGATGATGGCCACATTGGAGTTTTCGGTGGAGGATGCCTGTGTTTATCGAGGTTCTCATGGAAGGGGAGTAATTTTGTTTGTATTGATTTCTGGGTTATGAGAGAATATTGGGTGCCGGATTCATGGATTCAGCTCTTTAACTTGAAATTGGAGTATTCCGAGCAGATGACGAGCCTTTCGCATGTCAGACAATTCTTGTTTATGGAGAATAGTACAGTTGCTTTGGTGCAAGTGCCAAAAACGGGACCGTACATGAAACGTACGTGGGTTGATAAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

231

Amino Acids

26.49

Weight (kDa)

8.46

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 100 - 214 2.3e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021004)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09021
rosa_laevigata RLG00000016567
rosa_multiflora Rmu_sc0010847.1_g000002 Rmu_ssc0000183.1_g000047
rosa_roxburghii Rroxscaffold_2G00146340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 526
AciI CCGC 1 cut(s) 261
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 3 cut(s) 69, 103, 286
AfaI GTAC 4 cut(s) 182, 628, 665, 676
AfiI CCNNNNNNNGG 3 cut(s) 45, 198, 656
AgsI TTSAA 7 cut(s) 66, 74, 89, 188, 233, 304, 559
AjnI CCWGG 1 cut(s) 224
AluBI AGCT 1 cut(s) 547
AluI AGCT 1 cut(s) 547
Alw26I GTCTC 2 cut(s) 43, 302
AoxI GGCC 3 cut(s) 7, 221, 421
ApaI GGGCCC 1 cut(s) 11
ApoI RAATTY 3 cut(s) 69, 103, 286
ArsI GACNNNNNNTTYG 2 cut(s) 574, 606
AspS9I GGNCC 4 cut(s) 7, 8, 222, 659
AsuC2I CCSGG 1 cut(s) 240
AvaII GGWCC 1 cut(s) 659
BaeGI GKGCMC 1 cut(s) 11
BalI TGGCCA 1 cut(s) 423
BanI GGYRCC 1 cut(s) 526
BanII GRGCYC 1 cut(s) 11
BccI CCATC 2 cut(s) 47, 413
BceAI ACGGC 2 cut(s) 268, 274
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 1 cut(s) 226
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 2 cut(s) 43, 302
BfaI CTAG 4 cut(s) 24, 114, 122, 323
BisI GCNGC 1 cut(s) 262
BlsI GCNGC 1 cut(s) 263
Bme1390I CCNGG 2 cut(s) 226, 240
Bme18I GGWCC 1 cut(s) 659
BmgT120I GGNCC 4 cut(s) 7, 8, 222, 659
BmiI GGNNCC 3 cut(s) 9, 528, 660
BmrFI CCNGG 2 cut(s) 226, 240
BmsI GCATC 2 cut(s) 325, 437
BplI GAGNNNNNCTC 2 cut(s) 373, 405
BpuMI CCSGG 1 cut(s) 240
BsaAI YACGTR 1 cut(s) 678
BsaJI CCNNGG 3 cut(s) 58, 224, 238
BsaWI WCCGGW 1 cut(s) 198
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 198, 656
Bse3DI GCAATG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 226
BseDI CCNNGG 3 cut(s) 58, 224, 238
BseGI GGATG 2 cut(s) 340, 452
BseLI CCNNNNNNNGG 3 cut(s) 45, 198, 656
BseMI GCAATG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 11
BshFI GGCC 3 cut(s) 9, 223, 423
BshNI GGYRCC 1 cut(s) 526
BsiSI CCGG 3 cut(s) 199, 240, 530
BsiWI CGTACG 1 cut(s) 674
BslFI GGGAC 1 cut(s) 672
BslI CCNNNNNNNGG 3 cut(s) 45, 198, 656
BsmAI GTCTC 2 cut(s) 43, 302
BsmBI CGTCTC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 672
BsnI GGCC 3 cut(s) 9, 223, 423
Bsp120I GGGCCC 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 1 cut(s) 98
BspACI CCGC 1 cut(s) 261
BspANI GGCC 3 cut(s) 9, 223, 423
BspLI GGNNCC 3 cut(s) 9, 528, 660
BspT107I GGYRCC 1 cut(s) 526
BsrDI GCAATG 1 cut(s) 139
BssECI CCNNGG 3 cut(s) 58, 224, 238
BssMI GATC 1 cut(s) 98
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 4 cut(s) 205, 298, 631, 663
Bst6I CTCTTC 2 cut(s) 318, 360
BstBAI YACGTR 1 cut(s) 678
BstC8I GCNNGC 2 cut(s) 34, 221
BstF5I GGATG 2 cut(s) 340, 452
BstKTI GATC 1 cut(s) 101
BstMAI GTCTC 2 cut(s) 43, 302
BstMBI GATC 1 cut(s) 98
BstMWI GCNNNNNNNGC 2 cut(s) 261, 344
BstNI CCWGG 1 cut(s) 226
BstNSI RCATGY 1 cut(s) 599
BstSCI CCNGG 2 cut(s) 224, 238
BstSLI GKGCMC 1 cut(s) 11
BsuRI GGCC 3 cut(s) 9, 223, 423
BtgZI GCGATG 1 cut(s) 62
BtsCI GGATG 2 cut(s) 340, 452
Cac8I GCNNGC 2 cut(s) 34, 221
Cfr13I GGNCC 4 cut(s) 7, 8, 222, 659
Csp6I GTAC 4 cut(s) 181, 627, 664, 675
CviAII CATG 5 cut(s) 129, 471, 537, 596, 667
CviQI GTAC 4 cut(s) 181, 627, 664, 675
DpnI GATC 1 cut(s) 100
DpnII GATC 1 cut(s) 98
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 2 cut(s) 318, 360
EarI CTCTTC 2 cut(s) 318, 360
Eco24I GRGCYC 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 659
EcoO109I RGGNCCY 2 cut(s) 7, 8
EcoRI GAATTC 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 224
EcoT38I GRGCYC 1 cut(s) 11
Esp3I CGTCTC 1 cut(s) 43
FaeI CATG 5 cut(s) 132, 474, 540, 599, 670
FaqI GGGAC 1 cut(s) 672
FatI CATG 5 cut(s) 128, 470, 536, 595, 666
Fnu4HI GCNGC 1 cut(s) 262
FokI GGATG 2 cut(s) 347, 459
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 262
FspBI CTAG 4 cut(s) 24, 114, 122, 323
GluI GCNGC 1 cut(s) 262
HaeIII GGCC 3 cut(s) 9, 223, 423
HapII CCGG 3 cut(s) 199, 240, 530
Hin1II CATG 5 cut(s) 132, 474, 540, 599, 670
HinfI GANTC 6 cut(s) 18, 54, 233, 310, 533, 541
HpaII CCGG 3 cut(s) 199, 240, 530
Hpy166II GTNNAC 2 cut(s) 181, 196
Hpy188I TCNGA 3 cut(s) 48, 574, 602
Hpy188III TCNNGA 2 cut(s) 86, 114
Hpy8I GTNNAC 2 cut(s) 181, 196
Hpy99I CGWCG 4 cut(s) 155, 158, 161, 254
HpyAV CCTTC 3 cut(s) 203, 355, 469
HpyCH4III ACNGT 4 cut(s) 205, 298, 631, 663
HpyCH4IV ACGT 3 cut(s) 183, 673, 677
HpyCH4V TGCA 3 cut(s) 32, 132, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 344
HpySE526I ACGT 3 cut(s) 183, 673, 677
Hsp92II CATG 5 cut(s) 132, 474, 540, 599, 670
Kzo9I GATC 1 cut(s) 98
LpnPI CCDG 8 cut(s) 205, 211, 212, 238, 253, 464, 490, 543
LweI GCATC 2 cut(s) 325, 437
MaeI CTAG 4 cut(s) 24, 114, 122, 323
MaeII ACGT 3 cut(s) 183, 673, 677
MalI GATC 1 cut(s) 100
MboI GATC 1 cut(s) 98
MboII GAAGA 2 cut(s) 305, 377
MhlI GDGCHC 1 cut(s) 11
MlsI TGGCCA 1 cut(s) 423
MluCI AATT 8 cut(s) 69, 103, 213, 286, 403, 484, 560, 605
MluNI TGGCCA 1 cut(s) 423
MlyI GAGTC 1 cut(s) 304
MnlI CCTC 7 cut(s) 21, 24, 68, 399, 407, 437, 456
Mox20I TGGCCA 1 cut(s) 423
MscI TGGCCA 1 cut(s) 423
MseI TTAA 2 cut(s) 371, 552
Msp20I TGGCCA 1 cut(s) 423
MspI CCGG 3 cut(s) 199, 240, 530
MspR9I CCNGG 2 cut(s) 226, 240
MvaI CCWGG 1 cut(s) 226
MwoI GCNNNNNNNGC 2 cut(s) 261, 344
NciI CCSGG 1 cut(s) 240
NdeII GATC 1 cut(s) 98
NlaIII CATG 5 cut(s) 132, 474, 540, 599, 670
NlaIV GGNNCC 3 cut(s) 9, 528, 660
NspI RCATGY 1 cut(s) 599
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 189
PfeI GAWTC 5 cut(s) 18, 54, 233, 533, 541
Pfl23II CGTACG 1 cut(s) 674
PkrI GCNGC 1 cut(s) 263
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
Ppu21I YACGTR 1 cut(s) 678
Psp6I CCWGG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 224
PspLI CGTACG 1 cut(s) 674
PspN4I GGNNCC 3 cut(s) 9, 528, 660
PspOMI GGGCCC 1 cut(s) 7
PspPI GGNCC 4 cut(s) 7, 8, 222, 659
RsaI GTAC 4 cut(s) 182, 628, 665, 676
RsaNI GTAC 4 cut(s) 181, 627, 664, 675
SaqAI TTAA 2 cut(s) 371, 552
SatI GCNGC 1 cut(s) 262
Sau3AI GATC 1 cut(s) 98
Sau96I GGNCC 4 cut(s) 7, 8, 222, 659
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 2 cut(s) 226, 240
SduI GDGCHC 1 cut(s) 11
SetI ASST 7 cut(s) 127, 186, 391, 467, 549, 676, 680
SfaNI GCATC 2 cut(s) 325, 437
SinI GGWCC 1 cut(s) 659
Sse9I AATT 8 cut(s) 69, 103, 213, 286, 403, 484, 560, 605
SsiI CCGC 1 cut(s) 261
SspI AATATT 1 cut(s) 521
SspMI CTAG 4 cut(s) 24, 114, 122, 323
StyD4I CCNGG 2 cut(s) 224, 238
TaaI ACNGT 4 cut(s) 205, 298, 631, 663
TaiI ACGT 3 cut(s) 186, 676, 680
TaqI TCGA 5 cut(s) 16, 159, 207, 410, 461
TasI AATT 8 cut(s) 69, 103, 213, 286, 403, 484, 560, 605
TatI WGTACW 1 cut(s) 626
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 5 cut(s) 18, 54, 233, 533, 541
Tru1I TTAA 2 cut(s) 371, 552
Tru9I TTAA 2 cut(s) 371, 552
TspDTI ATGAA 5 cut(s) 17, 117, 305, 525, 683
VpaK11BI GGWCC 1 cut(s) 659
XapI RAATTY 3 cut(s) 69, 103, 286
XbaI TCTAGA 1 cut(s) 113
XceI RCATGY 1 cut(s) 599
XspI CTAG 4 cut(s) 24, 114, 122, 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.