FvH4_1g20240

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017226
Physical Location & Seq
Forward (+)
12482859 .. 12483861
1003 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20240.t1

Sequence Viewer

Length: 525 bp
ATGGGCTGGTCAGAATCAGAGAGAGGTCTCTGCAAGAAGCACCCGGAAGTGAAACAGTTCCCCGGCGCCGTCTGCTCCACATGTCTCAGTGAAAAGCTCTCTCAGCTCTACGCTCCCTCCTCTTTTTCCGACAGGAAAGTAACCGCAGATTTCTTAGGTTACGGCTTCCTCCCCATCCACTCTTGCTCTTCTTCTAGCTACTCCTCTGCCTCCTCATCTCCTCCGGTGACCAATCATGGTCGTCGAGGGCACCACAAGCGCAACGCGTCGGAGGTGATGGGCCGTTCGATGTCCTTCATGTTCAGCAGTGATGGGACCAAGCAGGGTGGAGGTCATCATCATGGGTTGATGAAGAAGAGCCGATCGGTTGCGGTGGTTACGAAGAGTGGCGCGAGTCATAGCTTTGGAGATCAGGCGGTGATGAAGAGCGGGAAGAAGAAACGAGGGTTTTGGTCGAAGCTGATTAAGGGGACGGGGAGGAAGACCAAGCAGGTTTTCAAGCACTCCAAGAGTGTTCTAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

175

Amino Acids

18.94

Weight (kDa)

10.35

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017226)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20240
prunus_persica Prupe.6G193200_v2.0.a1
pyrus_communis pycom15g28120
rosa_chinensis RchiOBHm_Chr2g0112381
rosa_laevigata RLG00000017972
rosa_multiflora Rmu_sc0004467.1_g000054
rosa_roxburghii Rroxscaffold_2G00131490
rosa_rugosa Rorug02G0180000
rosa_samantha Rh2AG233200 Rh2BG246700 Rh2CG237600 Rh2DG240800
rosa_wichuraiana Rw2G018060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 481
AccB1I GGYRCC 2 cut(s) 65, 249
AccBSI CCGCTC 1 cut(s) 429
AccII CGCG 2 cut(s) 266, 392
AciI CCGC 4 cut(s) 144, 371, 416, 429
AcyI GRCGYC 1 cut(s) 66
AflIII ACRYGT 2 cut(s) 80, 264
AgsI TTSAA 1 cut(s) 499
AjuI GAANNNNNNNTTGG 2 cut(s) 479, 511
AluBI AGCT 5 cut(s) 97, 106, 198, 402, 460
AluI AGCT 5 cut(s) 97, 106, 198, 402, 460
Alw26I GTCTC 2 cut(s) 32, 89
AoxI GGCC 1 cut(s) 280
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 3 cut(s) 68, 261, 392
AspS9I GGNCC 2 cut(s) 280, 315
AsuC2I CCSGG 2 cut(s) 44, 63
AsuHPI GGTGA 3 cut(s) 238, 286, 430
AvaII GGWCC 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 252
BanI GGYRCC 2 cut(s) 65, 249
BbsI GAAGAC 1 cut(s) 488
BccI CCATC 3 cut(s) 182, 271, 305
BceAI ACGGC 3 cut(s) 53, 178, 267
BcnI CCSGG 2 cut(s) 44, 63
BcoDI GTCTC 2 cut(s) 32, 89
BfaI CTAG 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 481
Bme1390I CCNGG 2 cut(s) 44, 63
Bme18I GGWCC 1 cut(s) 315
BmgT120I GGNCC 2 cut(s) 280, 315
BmiI GGNNCC 3 cut(s) 67, 251, 316
BmrFI CCNGG 2 cut(s) 44, 63
BpiI GAAGAC 1 cut(s) 488
BpuMI CCSGG 2 cut(s) 44, 63
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 61
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
BseDI CCNNGG 1 cut(s) 61
BseGI GGATG 1 cut(s) 174
BseMII CTCAG 2 cut(s) 100, 116
BseRI GAGGAG 4 cut(s) 109, 193, 202, 210
BseSI GKGCMC 1 cut(s) 252
Bsh1236I CGCG 2 cut(s) 266, 392
Bsh1285I CGRYCG 1 cut(s) 365
BshFI GGCC 1 cut(s) 282
BshNI GGYRCC 2 cut(s) 65, 249
BsiEI CGRYCG 1 cut(s) 365
BsiSI CCGG 3 cut(s) 44, 63, 224
BslFI GGGAC 2 cut(s) 328, 484
BsmAI GTCTC 2 cut(s) 32, 89
BsmFI GGGAC 2 cut(s) 328, 484
BsnI GGCC 1 cut(s) 282
Bso31I GGTCTC 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 2 cut(s) 362, 409
BspACI CCGC 4 cut(s) 144, 371, 416, 429
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 2 cut(s) 99, 115
BspFNI CGCG 2 cut(s) 266, 392
BspLI GGNNCC 3 cut(s) 67, 251, 316
BspMI ACCTGC 1 cut(s) 481
BspQI GCTCTTC 3 cut(s) 193, 350, 419
BspT107I GGYRCC 2 cut(s) 65, 249
BspTNI GGTCTC 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 429
BssECI CCNNGG 1 cut(s) 61
BssMI GATC 2 cut(s) 362, 409
BssNI GRCGYC 1 cut(s) 66
Bst4CI ACNGT 1 cut(s) 57
Bst6I CTCTTC 4 cut(s) 193, 350, 377, 419
BstACI GRCGYC 1 cut(s) 66
BstDEI CTNAG 4 cut(s) 86, 102, 154, 518
BstEII GGTNACC 1 cut(s) 226
BstF5I GGATG 1 cut(s) 174
BstFNI CGCG 2 cut(s) 266, 392
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 3 cut(s) 68, 261, 392
BstKTI GATC 2 cut(s) 365, 412
BstMAI GTCTC 2 cut(s) 32, 89
BstMBI GATC 2 cut(s) 362, 409
BstMCI CGRYCG 1 cut(s) 365
BstMWI GCNNNNNNNGC 3 cut(s) 72, 103, 256
BstNSI RCATGY 1 cut(s) 84
BstPI GGTNACC 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 42, 61
BstSLI GKGCMC 1 cut(s) 252
BstUI CGCG 2 cut(s) 266, 392
BstV2I GAAGAC 1 cut(s) 488
BsuRI GGCC 1 cut(s) 282
BtsCI GGATG 1 cut(s) 174
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 2 cut(s) 94, 313
BveI ACCTGC 1 cut(s) 481
CfoI GCGC 3 cut(s) 68, 261, 392
Cfr13I GGNCC 2 cut(s) 280, 315
CseI GACGC 1 cut(s) 255
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CviAII CATG 4 cut(s) 81, 236, 298, 341
CviJI RGCY 9 cut(s) 6, 97, 106, 165, 198, 282, 360, 402, 460
CviKI_1 RGCY 9 cut(s) 6, 97, 106, 165, 198, 282, 360, 402, 460
DdeI CTNAG 4 cut(s) 86, 102, 154, 518
DinI GGCGCC 1 cut(s) 67
DpnI GATC 2 cut(s) 364, 411
DpnII GATC 2 cut(s) 362, 409
Eam1104I CTCTTC 4 cut(s) 193, 350, 377, 419
EarI CTCTTC 4 cut(s) 193, 350, 377, 419
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 1 cut(s) 315
Eco91I GGTNACC 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 226
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
FaeI CATG 4 cut(s) 84, 239, 301, 344
FaiI YATR 5 cut(s) 82, 237, 299, 342, 399
FaqI GGGAC 2 cut(s) 328, 484
FatI CATG 4 cut(s) 80, 235, 297, 340
FauI CCCGC 1 cut(s) 422
FokI GGATG 1 cut(s) 161
FspBI CTAG 1 cut(s) 195
GlaI GCGC 3 cut(s) 67, 260, 391
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 1 cut(s) 282
HapII CCGG 3 cut(s) 44, 63, 224
HgaI GACGC 1 cut(s) 255
HhaI GCGC 3 cut(s) 68, 261, 392
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 4 cut(s) 84, 239, 301, 344
Hin6I GCGC 3 cut(s) 66, 259, 390
HinP1I GCGC 3 cut(s) 66, 259, 390
HinfI GANTC 2 cut(s) 14, 394
HpaII CCGG 3 cut(s) 44, 63, 224
HphI GGTGA 3 cut(s) 238, 286, 430
Hpy188I TCNGA 4 cut(s) 13, 19, 130, 271
Hpy99I CGWCG 2 cut(s) 246, 271
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 1 cut(s) 57
HpyCH4V TGCA 1 cut(s) 33
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 103, 256
HpyF3I CTNAG 4 cut(s) 86, 102, 154, 518
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 4 cut(s) 84, 239, 301, 344
HspAI GCGC 3 cut(s) 66, 259, 390
KasI GGCGCC 1 cut(s) 65
Kzo9I GATC 2 cut(s) 362, 409
LguI GCTCTTC 3 cut(s) 193, 350, 419
LmnI GCTCC 2 cut(s) 80, 118
LpnPI CCDG 7 cut(s) 57, 76, 118, 237, 308, 398, 476
MaeI CTAG 1 cut(s) 195
MaeIII GTNAC 4 cut(s) 139, 158, 226, 376
MalI GATC 2 cut(s) 364, 411
MbiI CCGCTC 1 cut(s) 429
MboI GATC 2 cut(s) 362, 409
MboII GAAGA 9 cut(s) 180, 183, 364, 367, 394, 436, 445, 448, 493
MhlI GDGCHC 1 cut(s) 252
MluI ACGCGT 1 cut(s) 264
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 2 cut(s) 153, 249
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 1 cut(s) 465
MslI CAYNNNNRTG 1 cut(s) 339
MspI CCGG 3 cut(s) 44, 63, 224
MspR9I CCNGG 2 cut(s) 44, 63
MvnI CGCG 2 cut(s) 266, 392
MwoI GCNNNNNNNGC 3 cut(s) 72, 103, 256
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 2 cut(s) 44, 63
NdeII GATC 2 cut(s) 362, 409
NlaIII CATG 4 cut(s) 84, 239, 301, 344
NlaIV GGNNCC 3 cut(s) 67, 251, 316
NmuCI GTSAC 1 cut(s) 226
NspI RCATGY 1 cut(s) 84
PciI ACATGT 1 cut(s) 80
PciSI GCTCTTC 3 cut(s) 193, 350, 419
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 14
Ple19I CGATCG 1 cut(s) 365
PleI GAGTC 1 cut(s) 402
PluTI GGCGCC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 402
PscI ACATGT 1 cut(s) 80
PspEI GGTNACC 1 cut(s) 226
PspN4I GGNNCC 3 cut(s) 67, 251, 316
PspPI GGNCC 2 cut(s) 280, 315
PvuI CGATCG 1 cut(s) 365
RseI CAYNNNNRTG 1 cut(s) 339
SapI GCTCTTC 3 cut(s) 193, 350, 419
SaqAI TTAA 1 cut(s) 465
Sau3AI GATC 2 cut(s) 362, 409
Sau96I GGNCC 2 cut(s) 280, 315
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 2 cut(s) 44, 63
SduI GDGCHC 1 cut(s) 252
SfoI GGCGCC 1 cut(s) 67
SinI GGWCC 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 339
SsiI CCGC 4 cut(s) 144, 371, 416, 429
SspDI GGCGCC 1 cut(s) 65
SspMI CTAG 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 42, 61
TaaI ACNGT 1 cut(s) 57
TaqI TCGA 3 cut(s) 244, 287, 455
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 2 cut(s) 94, 313
TseFI GTSAC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 3 cut(s) 286, 365, 437
TspRI CASTG 2 cut(s) 94, 313
VpaK11BI GGWCC 1 cut(s) 315
XceI RCATGY 1 cut(s) 84
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.