FvH4_2g09380

Diaminopimelate decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012138
Physical Location & Seq
Reverse (-)
8298260 .. 8302728
4469 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g09380.t1

Sequence Viewer

Length: 1440 bp
ATGGCGGCTGCACACCTTACCTTCAACCCTCCATCCTCCTTCTCCAAACCCCTAAACCACTCTCTCCCCCGCAATCCATTTTCCCGAAACTCCATTCTACCCTTCAAACCCTCCCTTAAACCCCTCCTCCTCAAAGCCGTCCTCACCCAAGAAGCCCCTCAAACGGTGCCGTTTAAGCACTGCTTCTCCAAATCCTCTGATGGGTTTCTCTACTGCGAGGGCCTCAAGGTCCAGGACGTCATGGAGTCCGTGGAGAAGCGCCCTTTCTACCTCTACAGCAAGCCCCAGATCACCCGAAACGTTGAGGCCTATAAAGACGCCTTGGAGGGTTTAAAGTCTGTTATTGGGTACGCCATTAAAGCCAACAATAACTTCAAGATTTTGGAGCATTTGAGGGGTTTAGGCTGTGGTGCTGTGCTCGTTAGTGGGAATGAGCTCAGGTTGGCTCTTCGAGCTGGGTTTGACCCAACTAGGTGTATTTTCAATGGGAATGGAAAGTTGTTGGATGACTTGGTTTTGGCTGCCCAAGAGGGTGTCTTTATCAATGTCGATAGTGAGTTCGACTTGGAGAATATTACCACCGCTGCGAGAATGGCTGGCAAGAGGGTCAATGTGCTACTTCGAATTAACCCGGATGTAGACCCTCAGGTCCATCCTTATGTATCCACTGGAAACAAGAATTCCAAATTTGGTATAAGAAATGAGAAGCTGCAATGGTTTTTGGATGAGGTTAAGAAACATCCTGATGAGCTAAAACTTGTTGGGGCCCATTGCCATCTTGGATCCACCATTACAAAGGTGGATATATTTCGAGATGCTGCAGTTCTTATGGTTGAGTACATTGACAAGATCAGAGATCAAGGTTTTGATGTTAATTACTTAAACATTGGTGGTGGTCTTGGGATAGATTACTATCATACTGGTGCTGTTCTTCCAACACCCAGAGATCTCATTAATACTGTTTGGGAGGTGGTCCTATCACGAGACCTAAATCTTATCATTGAACCCGGAAGGTCACTTATTGCAAATTCTTGCTGTCTGGTCAACCGAGTAACAGGGGTGAAAACGAATGGCACCAAAAACTTTATTGTGATCGATGGAAGCATGGCTGAACTCATTCGTCCCAGTCTCTATGATGCTTATCAACATATTGAACTAGTTGCTCCTGCACCGCATGAAGCTGAGATTTCTACTTTTGATGTGGTTGGTCCTGTCTGTGAGTCTGCAGATTTTTTAGGGAAGGAGAGAAAACTTCCAACTCCTCCAAAGGGAGCTGGTCTGGTTGTTCATGATGCAGGTGCTTACTGCATGAGCATGGCCTCGACTTACAATCTTAAAATGCGCCCTCCAGAGTATTGGGTTGAAGAAGATGGATCAGTGTCAAAGATCAGGCATGCAGAGACATTTAAAGATCATATGAGATTCTTTGAGGGTCTTTGA

Protein Analysis

480

Amino Acids

53.19

Weight (kDa)

6.92

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Orn_DAP_Arg_deC PF00278 90 - 433 1.7e-22 Pyridoxal-dependent decarboxylase, C-terminal sheet domain
Orn_Arg_deC_N PF02784 96 - 342 4.5e-49 Pyridoxal-dependent decarboxylase, pyridoxal binding domain
Ala_racemase_N PF01168 96 - 296 4.7e-06 Alanine racemase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012138)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14390 AT5G11880
fragaria_vesca FvH4_2g09380
malus_domestica MD05G1063100.v1.1 MD10G1070600.v1.1
prunus_persica Prupe.8G092300_v2.0.a1 Prupe.8G092300_v2.0.a1
pyrus_communis pycom05g05450 pycom10g05640
rosa_chinensis RchiOBHm_Chr6g0265651
rosa_laevigata RLG00000014169
rosa_multiflora Rmu_sc0003233.1_g000010
rosa_roxburghii Rroxscaffold_175G00432470 Rroxscaffold_7G00202530
rosa_rugosa Rorug06G0018200
rosa_samantha Rh6AG140300 Rh6BG140100 Rh6CG137200
rosa_wichuraiana Rw6G012110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1286
AasI GACNNNNNNGTC 1 cut(s) 647
AatII GACGTC 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 1286
AccB1I GGYRCC 2 cut(s) 166, 1073
AccI GTMKAC 1 cut(s) 639
AciI CCGC 4 cut(s) 5, 70, 582, 1172
AclI AACGTT 1 cut(s) 300
AclWI GGATC 3 cut(s) 777, 790, 1381
AcsI RAATTY 3 cut(s) 679, 686, 1027
AcyI GRCGYC 2 cut(s) 237, 318
AfaI GTAC 2 cut(s) 350, 839
AfiI CCNNNNNNNGG 3 cut(s) 163, 201, 1268
AgsI TTSAA 7 cut(s) 25, 106, 376, 484, 1004, 1154, 1364
AhlI ACTAGT 1 cut(s) 1156
AjnI CCWGG 1 cut(s) 231
AjuI GAANNNNNNNTTGG 2 cut(s) 356, 388
AluBI AGCT 6 cut(s) 436, 455, 709, 751, 1181, 1274
AluI AGCT 6 cut(s) 436, 455, 709, 751, 1181, 1274
Alw21I GWGCWC 2 cut(s) 420, 438
Alw26I GTCTC 3 cut(s) 978, 1133, 1394
AlwI GGATC 3 cut(s) 777, 790, 1381
AoxI GGCC 4 cut(s) 220, 306, 765, 1317
ApaI GGGCCC 1 cut(s) 769
ApeKI GCWGC 5 cut(s) 8, 521, 584, 709, 818
ApoI RAATTY 3 cut(s) 679, 686, 1027
AseI ATTAAT 1 cut(s) 954
Asp700I GAANNNNTTC 1 cut(s) 1116
AspLEI GCGC 2 cut(s) 261, 1344
AspS9I GGNCC 7 cut(s) 220, 229, 649, 765, 766, 973, 1208
AsuC2I CCSGG 2 cut(s) 632, 1008
AsuHPI GGTGA 3 cut(s) 136, 283, 1072
AsuII TTCGAA 1 cut(s) 622
AvaII GGWCC 4 cut(s) 229, 649, 973, 1208
AxyI CCTNAGG 1 cut(s) 645
BaeGI GKGCMC 1 cut(s) 769
BamHI GGATCC 1 cut(s) 782
BanI GGYRCC 2 cut(s) 166, 1073
BanII GRGCYC 2 cut(s) 438, 769
BauI CACGAG 1 cut(s) 981
Bbv12I GWGCWC 2 cut(s) 420, 438
BbvI GCAGC 4 cut(s) 508, 571, 696, 805
BccI CCATC 6 cut(s) 40, 194, 660, 783, 1091, 1364
BceAI ACGGC 2 cut(s) 122, 154
BciT130I CCWGG 1 cut(s) 233
BciVI GTATCC 1 cut(s) 673
BcnI CCSGG 2 cut(s) 632, 1008
BcoDI GTCTC 3 cut(s) 978, 1133, 1394
BcuI ACTAGT 1 cut(s) 1156
BfaI CTAG 2 cut(s) 471, 1157
BfmI CTRYAG 3 cut(s) 274, 819, 1224
BfoI RGCGCY 1 cut(s) 262
BfuAI ACCTGC 1 cut(s) 1286
BfuI GTATCC 1 cut(s) 673
BglII AGATCT 1 cut(s) 946
BisI GCNGC 6 cut(s) 6, 9, 522, 585, 710, 819
BlsI GCNGC 6 cut(s) 7, 10, 523, 586, 711, 820
Bme1390I CCNGG 3 cut(s) 233, 632, 1008
Bme18I GGWCC 4 cut(s) 229, 649, 973, 1208
BmgT120I GGNCC 7 cut(s) 220, 229, 649, 765, 766, 973, 1208
BmiI GGNNCC 5 cut(s) 168, 766, 767, 784, 1075
BmrFI CCNGG 3 cut(s) 233, 632, 1008
BmrI ACTGGG 1 cut(s) 1119
BmsI GCATC 3 cut(s) 805, 1126, 1282
BmuI ACTGGG 1 cut(s) 1119
BpmI CTGGAG 1 cut(s) 1332
Bpu10I CCTNAGC 1 cut(s) 437
Bpu14I TTCGAA 1 cut(s) 622
BpuEI CTTGAG 1 cut(s) 209
BpuMI CCSGG 2 cut(s) 632, 1008
Bsa29I ATCGAT 1 cut(s) 1095
BsaBI GATNNNNATC 2 cut(s) 912, 1140
BsaHI GRCGYC 2 cut(s) 237, 318
BsaI GGTCTC 1 cut(s) 978
BsaJI CCNNGG 2 cut(s) 249, 321
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 170, 200
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 201, 1268
Bse1I ACTGG 3 cut(s) 673, 925, 1125
Bse21I CCTNAGG 1 cut(s) 645
Bse3DI GCAATG 2 cut(s) 719, 769
Bse8I GATNNNNATC 2 cut(s) 912, 1140
BseBI CCWGG 1 cut(s) 233
BseCI ATCGAT 1 cut(s) 1095
BseDI CCNNGG 2 cut(s) 249, 321
BseGI GGATG 6 cut(s) 32, 511, 640, 652, 730, 739
BseJI GATNNNNATC 2 cut(s) 912, 1140
BseLI CCNNNNNNNGG 3 cut(s) 163, 201, 1268
BseMI GCAATG 2 cut(s) 719, 769
BseMII CTCAG 3 cut(s) 451, 659, 1173
BseNI ACTGG 3 cut(s) 673, 925, 1125
BseRI GAGGAG 3 cut(s) 116, 119, 1251
BseSI GKGCMC 1 cut(s) 769
BseXI GCAGC 4 cut(s) 508, 571, 696, 805
BseYI CCCAGC 1 cut(s) 455
BsgI GTGCAG 1 cut(s) 1152
BshFI GGCC 4 cut(s) 222, 308, 767, 1319
BshNI GGYRCC 2 cut(s) 166, 1073
BshVI ATCGAT 1 cut(s) 1095
BsiHKAI GWGCWC 2 cut(s) 420, 438
BsiSI CCGG 2 cut(s) 632, 1008
BslFI GGGAC 1 cut(s) 1107
BslI CCNNNNNNNGG 3 cut(s) 163, 201, 1268
BsmAI GTCTC 3 cut(s) 978, 1133, 1394
BsmFI GGGAC 1 cut(s) 1107
BsnI GGCC 4 cut(s) 222, 308, 767, 1319
Bso31I GGTCTC 1 cut(s) 978
Bsp119I TTCGAA 1 cut(s) 622
Bsp120I GGGCCC 1 cut(s) 765
Bsp1286I GDGCHC 3 cut(s) 420, 438, 769
Bsp143I GATC 9 cut(s) 288, 782, 849, 856, 946, 1092, 1373, 1386, 1411
BspACI CCGC 4 cut(s) 5, 70, 582, 1172
BspANI GGCC 4 cut(s) 222, 308, 767, 1319
BspCNI CTCAG 3 cut(s) 450, 658, 1174
BspDI ATCGAT 1 cut(s) 1095
BspHI TCATGA 1 cut(s) 1288
BspLI GGNNCC 5 cut(s) 168, 766, 767, 784, 1075
BspMAI CTGCAG 2 cut(s) 823, 1228
BspMI ACCTGC 1 cut(s) 1286
BspPI GGATC 3 cut(s) 777, 790, 1381
BspQI GCTCTTC 1 cut(s) 453
BspT104I TTCGAA 1 cut(s) 622
BspT107I GGYRCC 2 cut(s) 166, 1073
BspTNI GGTCTC 1 cut(s) 978
BsrDI GCAATG 2 cut(s) 719, 769
BsrI ACTGG 3 cut(s) 673, 925, 1125
BssECI CCNNGG 2 cut(s) 249, 321
BssMI GATC 9 cut(s) 288, 782, 849, 856, 946, 1092, 1373, 1386, 1411
BssNI GRCGYC 2 cut(s) 237, 318
BssSI CACGAG 1 cut(s) 981
BssT1I CCWWGG 1 cut(s) 321
Bst2BI CACGAG 1 cut(s) 981
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 2 cut(s) 166, 961
Bst6I CTCTTC 1 cut(s) 453
BstACI GRCGYC 2 cut(s) 237, 318
BstBI TTCGAA 1 cut(s) 622
BstC8I GCNNGC 3 cut(s) 281, 598, 1395
BstDEI CTNAG 3 cut(s) 437, 645, 1182
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 6 cut(s) 32, 511, 640, 652, 730, 739
BstH2I RGCGCY 1 cut(s) 262
BstHHI GCGC 2 cut(s) 261, 1344
BstKTI GATC 9 cut(s) 291, 785, 852, 859, 949, 1095, 1376, 1389, 1414
BstMAI GTCTC 3 cut(s) 978, 1133, 1394
BstMBI GATC 9 cut(s) 288, 782, 849, 856, 946, 1092, 1373, 1386, 1411
BstMWI GCNNNNNNNGC 4 cut(s) 175, 359, 452, 593
BstNI CCWGG 1 cut(s) 233
BstNSI RCATGY 1 cut(s) 1397
BstSCI CCNGG 3 cut(s) 231, 630, 1006
BstSFI CTRYAG 3 cut(s) 274, 819, 1224
BstSLI GKGCMC 1 cut(s) 769
BstV1I GCAGC 4 cut(s) 508, 571, 696, 805
BstX2I RGATCY 2 cut(s) 782, 946
BstXI CCANNNNNNTGG 1 cut(s) 1356
BstYI RGATCY 2 cut(s) 782, 946
Bsu15I ATCGAT 1 cut(s) 1095
Bsu36I CCTNAGG 1 cut(s) 645
BsuI GTATCC 1 cut(s) 673
BsuRI GGCC 4 cut(s) 222, 308, 767, 1319
BsuTUI ATCGAT 1 cut(s) 1095
BtgI CCRYGG 1 cut(s) 249
BtsCI GGATG 6 cut(s) 32, 511, 640, 652, 730, 739
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 3 cut(s) 178, 666, 1383
BveI ACCTGC 1 cut(s) 1286
Cac8I GCNNGC 3 cut(s) 281, 598, 1395
CciI TCATGA 1 cut(s) 1288
CfoI GCGC 2 cut(s) 261, 1344
Cfr13I GGNCC 7 cut(s) 220, 229, 649, 765, 766, 973, 1208
ClaI ATCGAT 1 cut(s) 1095
CseI GACGC 1 cut(s) 326
Csp6I GTAC 2 cut(s) 349, 838
CviAII CATG 7 cut(s) 241, 1105, 1175, 1289, 1309, 1315, 1394
CviQI GTAC 2 cut(s) 349, 838
DdeI CTNAG 3 cut(s) 437, 645, 1182
DpnI GATC 9 cut(s) 290, 784, 851, 858, 948, 1094, 1375, 1388, 1413
DpnII GATC 9 cut(s) 288, 782, 849, 856, 946, 1092, 1373, 1386, 1411
DraI TTTAAA 2 cut(s) 333, 1408
DrdI GACNNNNNNGTC 1 cut(s) 647
DseDI GACNNNNNNGTC 1 cut(s) 647
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
Ecl136II GAGCTC 1 cut(s) 436
Eco130I CCWWGG 1 cut(s) 321
Eco147I AGGCCT 1 cut(s) 308
Eco24I GRGCYC 2 cut(s) 438, 769
Eco31I GGTCTC 1 cut(s) 978
Eco47I GGWCC 4 cut(s) 229, 649, 973, 1208
Eco53kI GAGCTC 1 cut(s) 436
Eco81I CCTNAGG 1 cut(s) 645
EcoICRI GAGCTC 1 cut(s) 436
EcoO109I RGGNCCY 2 cut(s) 220, 765
EcoRI GAATTC 1 cut(s) 679
EcoRII CCWGG 1 cut(s) 231
EcoT14I CCWWGG 1 cut(s) 321
EcoT38I GRGCYC 2 cut(s) 438, 769
ErhI CCWWGG 1 cut(s) 321
FaeI CATG 7 cut(s) 244, 1108, 1178, 1292, 1312, 1318, 1397
FalI AAGNNNNNCTT 2 cut(s) 167, 199
FaqI GGGAC 1 cut(s) 1107
FatI CATG 7 cut(s) 240, 1104, 1174, 1288, 1308, 1314, 1393
FauI CCCGC 1 cut(s) 77
FauNDI CATATG 1 cut(s) 1416
FblI GTMKAC 1 cut(s) 639
Fnu4HI GCNGC 6 cut(s) 6, 9, 522, 585, 710, 819
FokI GGATG 6 cut(s) 19, 518, 639, 647, 726, 737
FriOI GRGCYC 2 cut(s) 438, 769
Fsp4HI GCNGC 6 cut(s) 6, 9, 522, 585, 710, 819
FspBI CTAG 2 cut(s) 471, 1157
GlaI GCGC 2 cut(s) 260, 1343
GluI GCNGC 6 cut(s) 6, 9, 522, 585, 710, 819
GsaI CCCAGC 1 cut(s) 459
GsuI CTGGAG 1 cut(s) 1332
HaeII RGCGCY 1 cut(s) 262
HaeIII GGCC 4 cut(s) 222, 308, 767, 1319
HapII CCGG 2 cut(s) 632, 1008
HgaI GACGC 1 cut(s) 326
HhaI GCGC 2 cut(s) 261, 1344
Hin1I GRCGYC 2 cut(s) 237, 318
Hin1II CATG 7 cut(s) 244, 1108, 1178, 1292, 1312, 1318, 1397
Hin6I GCGC 2 cut(s) 259, 1342
HinP1I GCGC 2 cut(s) 259, 1342
HincII GTYRAC 1 cut(s) 1045
HindII GTYRAC 1 cut(s) 1045
HinfI GANTC 3 cut(s) 245, 1220, 1422
HpaII CCGG 2 cut(s) 632, 1008
HphI GGTGA 3 cut(s) 136, 283, 1072
Hpy166II GTNNAC 2 cut(s) 640, 1045
Hpy188I TCNGA 2 cut(s) 199, 854
Hpy188III TCNNGA 7 cut(s) 84, 376, 743, 812, 981, 1289, 1349
Hpy8I GTNNAC 2 cut(s) 640, 1045
HpyAV CCTTC 5 cut(s) 31, 49, 112, 1005, 1234
HpyCH4III ACNGT 2 cut(s) 166, 961
HpyCH4IV ACGT 2 cut(s) 237, 300
HpyCH4V TGCA 9 cut(s) 11, 712, 821, 1025, 1169, 1226, 1295, 1308, 1397
HpyF10VI GCNNNNNNNGC 4 cut(s) 175, 359, 452, 593
HpyF3I CTNAG 3 cut(s) 437, 645, 1182
HpySE526I ACGT 2 cut(s) 237, 300
Hsp92I GRCGYC 2 cut(s) 237, 318
Hsp92II CATG 7 cut(s) 244, 1108, 1178, 1292, 1312, 1318, 1397
HspAI GCGC 2 cut(s) 259, 1342
Kzo9I GATC 9 cut(s) 288, 782, 849, 856, 946, 1092, 1373, 1386, 1411
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 3 cut(s) 385, 1168, 1271
Lsp1109I GCAGC 4 cut(s) 508, 571, 696, 805
LweI GCATC 3 cut(s) 805, 1126, 1282
MaeI CTAG 2 cut(s) 471, 1157
MaeII ACGT 2 cut(s) 237, 300
MaeIII GTNAC 2 cut(s) 1014, 1051
MalI GATC 9 cut(s) 290, 784, 851, 858, 948, 1094, 1375, 1388, 1413
MboI GATC 9 cut(s) 288, 782, 849, 856, 946, 1092, 1373, 1386, 1411
MboII GAAGA 4 cut(s) 440, 923, 1376, 1379
MflI RGATCY 2 cut(s) 782, 946
MhlI GDGCHC 3 cut(s) 420, 438, 769
MluCI AATT 5 cut(s) 624, 679, 686, 874, 1027
MlyI GAGTC 2 cut(s) 254, 1229
MmeI TCCRAC 3 cut(s) 483, 959, 1280
MroXI GAANNNNTTC 1 cut(s) 1116
MslI CAYNNNNRTG 4 cut(s) 657, 744, 921, 1313
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 2 cut(s) 632, 1008
MspR9I CCNGG 3 cut(s) 233, 632, 1008
MvaI CCWGG 1 cut(s) 233
MwoI GCNNNNNNNGC 4 cut(s) 175, 359, 452, 593
NciI CCSGG 2 cut(s) 632, 1008
NdeI CATATG 1 cut(s) 1416
NdeII GATC 9 cut(s) 288, 782, 849, 856, 946, 1092, 1373, 1386, 1411
NlaIII CATG 7 cut(s) 244, 1108, 1178, 1292, 1312, 1318, 1397
NlaIV GGNNCC 5 cut(s) 168, 766, 767, 784, 1075
NmuCI GTSAC 1 cut(s) 1014
NspI RCATGY 1 cut(s) 1397
NspV TTCGAA 1 cut(s) 622
PaeI GCATGC 1 cut(s) 1397
PagI TCATGA 1 cut(s) 1288
PaqCI CACCTGC 1 cut(s) 1286
PceI AGGCCT 1 cut(s) 308
PciSI GCTCTTC 1 cut(s) 453
PdmI GAANNNNTTC 1 cut(s) 1116
PfeI GAWTC 1 cut(s) 1422
PfoI TCCNGGA 1 cut(s) 231
PkrI GCNGC 6 cut(s) 7, 10, 523, 586, 711, 820
PleI GAGTC 2 cut(s) 253, 1228
PpsI GAGTC 2 cut(s) 253, 1228
PshBI ATTAAT 1 cut(s) 954
Psp124BI GAGCTC 1 cut(s) 438
Psp1406I AACGTT 1 cut(s) 300
Psp6I CCWGG 1 cut(s) 231
PspFI CCCAGC 1 cut(s) 455
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 5 cut(s) 168, 766, 767, 784, 1075
PspOMI GGGCCC 1 cut(s) 765
PspPI GGNCC 7 cut(s) 220, 229, 649, 765, 766, 973, 1208
PstI CTGCAG 2 cut(s) 823, 1228
PsuI RGATCY 2 cut(s) 782, 946
RsaI GTAC 2 cut(s) 350, 839
RsaNI GTAC 2 cut(s) 349, 838
RseI CAYNNNNRTG 4 cut(s) 657, 744, 921, 1313
SacI GAGCTC 1 cut(s) 438
SapI GCTCTTC 1 cut(s) 453
SatI GCNGC 6 cut(s) 6, 9, 522, 585, 710, 819
Sau3AI GATC 9 cut(s) 288, 782, 849, 856, 946, 1092, 1373, 1386, 1411
Sau96I GGNCC 7 cut(s) 220, 229, 649, 765, 766, 973, 1208
SchI GAGTC 2 cut(s) 254, 1229
ScrFI CCNGG 3 cut(s) 233, 632, 1008
SduI GDGCHC 3 cut(s) 420, 438, 769
SfaNI GCATC 3 cut(s) 805, 1126, 1282
SfcI CTRYAG 3 cut(s) 274, 819, 1224
SfuI TTCGAA 1 cut(s) 622
SinI GGWCC 4 cut(s) 229, 649, 973, 1208
SmiMI CAYNNNNRTG 4 cut(s) 657, 744, 921, 1313
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SpeI ACTAGT 1 cut(s) 1156
SphI GCATGC 1 cut(s) 1397
Sse9I AATT 5 cut(s) 624, 679, 686, 874, 1027
SseBI AGGCCT 1 cut(s) 308
SsiI CCGC 4 cut(s) 5, 70, 582, 1172
SspI AATATT 1 cut(s) 574
SspMI CTAG 2 cut(s) 471, 1157
SstI GAGCTC 1 cut(s) 438
StuI AGGCCT 1 cut(s) 308
StyD4I CCNGG 3 cut(s) 231, 630, 1006
StyI CCWWGG 1 cut(s) 321
TaaI ACNGT 2 cut(s) 166, 961
TaiI ACGT 2 cut(s) 240, 303
TaqI TCGA 7 cut(s) 451, 549, 561, 622, 811, 1095, 1322
TasI AATT 5 cut(s) 624, 679, 686, 874, 1027
TatI WGTACW 1 cut(s) 837
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 1422
TscAI CASTG 3 cut(s) 185, 673, 1383
TseFI GTSAC 1 cut(s) 1014
TseI GCWGC 5 cut(s) 8, 521, 584, 709, 818
Tsp45I GTSAC 1 cut(s) 1014
TspDTI ATGAA 2 cut(s) 1191, 1277
TspGWI ACGGA 1 cut(s) 238
TspRI CASTG 3 cut(s) 185, 673, 1383
VpaK11BI GGWCC 4 cut(s) 229, 649, 973, 1208
VspI ATTAAT 1 cut(s) 954
XapI RAATTY 3 cut(s) 679, 686, 1027
XceI RCATGY 1 cut(s) 1397
XcmI CCANNNNNNNNNTGG 2 cut(s) 776, 796
XmiI GTMKAC 1 cut(s) 639
XmnI GAANNNNTTC 1 cut(s) 1116
XspI CTAG 2 cut(s) 471, 1157
ZraI GACGTC 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.