Rh6AG140300

Diaminopimelate decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0012138
Physical Location & Seq
Reverse (-)
21568161 .. 21572769
4609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG140300.1

Sequence Viewer

Length: 1203 bp
ATGGCGGCTGCGCAGCTTACCTTCAACCCTCCATCCTCCTTCTCCAAACCCCTAAACCACTGTCTCCCCCGAAACCCATTTTCCCAAAACCCCATTCTACCCTTCAAACCCTCCCTTAAACCCTTCGTTCTCAAAGCCGTGCTCTCCCAAAACCCTTCCAAATCCCTAACCCAGGACCCTCAAACGGTGCCGTTTAAGCACTGCTTCTCCAAATCCTCTGATGGGTTTCTCTACTGTGAGGGCCTCAAGGTCCAGGACGTCATGGATTCGGTGGAGAAGCGCCCTTTCTACCTCTACAGCAAGCCTCAGATCACCAGAAATGTTGAGGCCTATAAGGAGGCCTTGGAGGGTTTAAGGTCTGTTATTGGGTATGCCATTAAAGCCAACAATAACTTCAAGATTTTGGAGCATTTGAGGGGATTAGGCTGTGGTGCTGTGCTCGTCAGTGGGAACGAGCTCAGGTTGGCTCTTCGAGCTGGGTTTGACCCAACTAGGTGTATTTTCAATGGGAATGGAAAATTTTTGGATGACTTGGTTTTGGCTGCTCAAGAAGGTGTTTTTATCAATGTCGATAGTGAGTTCGACTTGGAAAATATTATTAGAGCTGCGAGAATTGCTGGCAAGAGGGTCAATATTCTACTTCGAATTAACCCAGATGTAGACCCTCAGGTTCATCCTTATGTTTCCACTGGTAACAAGAATTCCAAATTTGGTATTAGAAATGAGAAGCTGCAATGGTTTTTGGATGAGGTGAAGAAACATCCTGATGAGCTAAAACTTGTTGGGGCCCATTGCCATCTTGGATCCACCATTACAAAGGTGGATATATTTCGAGATGCTGCAGTTCTTATGGTCGAGTACCTTGACAAAATCCGAGATCAAGGTTTTGATGTTAATTACTTAAACATTGGTGGTGGTCTTGGGATAGATTACTATCATACTGGTGCTGTTCTTCCAACACCCAGAGATCTCATTAATACTGTCCGGGAGGTGGTCCTATCACGAGATCTAAATCTTATCATTGAACCCGGAAGATCACTTATTGCAAATTCTTGCTGTTTGGTCAACCGAGTAACTGGGGTGAAAACAAATGGCACCAAAAACTTTATTGTGATCGATGGAAGCATGGCTGAACTCATTCGTCCCAGTCTCTATGATGCTTATCAAGTAAGTTCTAAGTTTATTCAACTAATGTTGAATTAA

Protein Analysis

400

Amino Acids

44.55

Weight (kDa)

8.87

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Orn_DAP_Arg_deC PF00278 97 - 390 4.3e-08 Pyridoxal-dependent decarboxylase, C-terminal sheet domain
Orn_Arg_deC_N PF02784 103 - 349 2.9e-49 Pyridoxal-dependent decarboxylase, pyridoxal binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012138)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14390 AT5G11880
fragaria_vesca FvH4_2g09380
malus_domestica MD05G1063100.v1.1 MD10G1070600.v1.1
prunus_persica Prupe.8G092300_v2.0.a1 Prupe.8G092300_v2.0.a1
pyrus_communis pycom05g05450 pycom10g05640
rosa_chinensis RchiOBHm_Chr6g0265651
rosa_laevigata RLG00000014169
rosa_multiflora Rmu_sc0003233.1_g000010
rosa_roxburghii Rroxscaffold_175G00432470 Rroxscaffold_7G00202530
rosa_rugosa Rorug06G0018200
rosa_samantha Rh6AG140300 Rh6BG140100 Rh6CG137200
rosa_wichuraiana Rw6G012110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
Acc16I TGCGCA 1 cut(s) 12
AccB1I GGYRCC 2 cut(s) 187, 1094
AccI GTMKAC 1 cut(s) 660
AciI CCGC 1 cut(s) 5
AclWI GGATC 2 cut(s) 798, 811
AcsI RAATTY 4 cut(s) 518, 700, 707, 1048
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 1 cut(s) 860
AfiI CCNNNNNNNGG 4 cut(s) 172, 184, 222, 991
AgsI TTSAA 7 cut(s) 25, 106, 397, 505, 1025, 1187, 1198
AjnI CCWGG 2 cut(s) 171, 252
AjuI GAANNNNNNNTTGG 4 cut(s) 78, 110, 377, 409
AluBI AGCT 6 cut(s) 16, 457, 476, 605, 730, 772
AluI AGCT 6 cut(s) 16, 457, 476, 605, 730, 772
Alw21I GWGCWC 3 cut(s) 144, 441, 459
Alw26I GTCTC 2 cut(s) 68, 1154
AlwI GGATC 2 cut(s) 798, 811
AoxI GGCC 4 cut(s) 241, 327, 339, 786
ApaI GGGCCC 1 cut(s) 790
ApeKI GCWGC 6 cut(s) 8, 13, 542, 605, 730, 839
ApoI RAATTY 4 cut(s) 518, 700, 707, 1048
AseI ATTAAT 1 cut(s) 975
Asp700I GAANNNNTTC 1 cut(s) 1137
AspLEI GCGC 2 cut(s) 13, 282
AspS9I GGNCC 6 cut(s) 175, 241, 250, 786, 787, 994
AsuC2I CCSGG 2 cut(s) 986, 1029
AsuHPI GGTGA 3 cut(s) 304, 763, 1093
AsuII TTCGAA 1 cut(s) 643
AvaII GGWCC 3 cut(s) 175, 250, 994
AxyI CCTNAGG 1 cut(s) 666
BaeGI GKGCMC 1 cut(s) 790
BamHI GGATCC 1 cut(s) 803
BanI GGYRCC 2 cut(s) 187, 1094
BanII GRGCYC 2 cut(s) 459, 790
BauI CACGAG 1 cut(s) 1002
Bbv12I GWGCWC 3 cut(s) 144, 441, 459
BbvI GCAGC 5 cut(s) 25, 529, 592, 717, 826
BccI CCATC 4 cut(s) 40, 215, 804, 1112
BceAI ACGGC 2 cut(s) 122, 175
BciT130I CCWGG 2 cut(s) 173, 254
BcnI CCSGG 2 cut(s) 986, 1029
BcoDI GTCTC 2 cut(s) 68, 1154
BfaI CTAG 1 cut(s) 492
BfmI CTRYAG 2 cut(s) 295, 840
BfoI RGCGCY 1 cut(s) 283
BglII AGATCT 2 cut(s) 967, 1006
BisI GCNGC 7 cut(s) 6, 9, 14, 543, 606, 731, 840
BlsI GCNGC 7 cut(s) 7, 10, 15, 544, 607, 732, 841
Bme1390I CCNGG 4 cut(s) 173, 254, 986, 1029
Bme18I GGWCC 3 cut(s) 175, 250, 994
BmgT120I GGNCC 6 cut(s) 175, 241, 250, 786, 787, 994
BmiI GGNNCC 6 cut(s) 177, 189, 787, 788, 805, 1096
BmrFI CCNGG 4 cut(s) 173, 254, 986, 1029
BmrI ACTGGG 2 cut(s) 1086, 1140
BmsI GCATC 2 cut(s) 826, 1147
BmuI ACTGGG 2 cut(s) 1086, 1140
Bpu10I CCTNAGC 1 cut(s) 458
Bpu14I TTCGAA 1 cut(s) 643
BpuEI CTTGAG 2 cut(s) 230, 531
BpuMI CCSGG 2 cut(s) 986, 1029
Bsa29I ATCGAT 1 cut(s) 1116
BsaBI GATNNNNATC 3 cut(s) 933, 1011, 1161
BsaHI GRCGYC 1 cut(s) 258
BsaJI CCNNGG 2 cut(s) 171, 342
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 184, 222, 991
Bse1I ACTGG 4 cut(s) 694, 946, 1081, 1146
Bse21I CCTNAGG 1 cut(s) 666
Bse3DI GCAATG 2 cut(s) 740, 790
Bse8I GATNNNNATC 3 cut(s) 933, 1011, 1161
BseBI CCWGG 2 cut(s) 173, 254
BseCI ATCGAT 1 cut(s) 1116
BseDI CCNNGG 2 cut(s) 171, 342
BseGI GGATG 5 cut(s) 32, 532, 673, 751, 760
BseJI GATNNNNATC 3 cut(s) 933, 1011, 1161
BseLI CCNNNNNNNGG 4 cut(s) 172, 184, 222, 991
BseMI GCAATG 2 cut(s) 740, 790
BseMII CTCAG 3 cut(s) 320, 472, 680
BseNI ACTGG 4 cut(s) 694, 946, 1081, 1146
BseSI GKGCMC 1 cut(s) 790
BseXI GCAGC 5 cut(s) 25, 529, 592, 717, 826
BseYI CCCAGC 1 cut(s) 476
BshFI GGCC 4 cut(s) 243, 329, 341, 788
BshNI GGYRCC 2 cut(s) 187, 1094
BshVI ATCGAT 1 cut(s) 1116
BsiHKAI GWGCWC 3 cut(s) 144, 441, 459
BsiSI CCGG 2 cut(s) 985, 1029
BslFI GGGAC 1 cut(s) 1128
BslI CCNNNNNNNGG 4 cut(s) 172, 184, 222, 991
BsmAI GTCTC 2 cut(s) 68, 1154
BsmFI GGGAC 1 cut(s) 1128
BsnI GGCC 4 cut(s) 243, 329, 341, 788
Bsp119I TTCGAA 1 cut(s) 643
Bsp120I GGGCCC 1 cut(s) 786
Bsp1286I GDGCHC 4 cut(s) 144, 441, 459, 790
Bsp143I GATC 7 cut(s) 309, 803, 877, 967, 1006, 1034, 1113
BspACI CCGC 1 cut(s) 5
BspANI GGCC 4 cut(s) 243, 329, 341, 788
BspCNI CTCAG 3 cut(s) 319, 471, 679
BspDI ATCGAT 1 cut(s) 1116
BspLI GGNNCC 6 cut(s) 177, 189, 787, 788, 805, 1096
BspMAI CTGCAG 1 cut(s) 844
BspPI GGATC 2 cut(s) 798, 811
BspQI GCTCTTC 1 cut(s) 474
BspT104I TTCGAA 1 cut(s) 643
BspT107I GGYRCC 2 cut(s) 187, 1094
BsrDI GCAATG 2 cut(s) 740, 790
BsrI ACTGG 4 cut(s) 694, 946, 1081, 1146
BssECI CCNNGG 2 cut(s) 171, 342
BssMI GATC 7 cut(s) 309, 803, 877, 967, 1006, 1034, 1113
BssNI GRCGYC 1 cut(s) 258
BssSI CACGAG 1 cut(s) 1002
BssT1I CCWWGG 1 cut(s) 342
Bst2BI CACGAG 1 cut(s) 1002
Bst2UI CCWGG 2 cut(s) 173, 254
Bst4CI ACNGT 4 cut(s) 62, 187, 236, 982
Bst6I CTCTTC 1 cut(s) 474
BstACI GRCGYC 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 643
BstC8I GCNNGC 2 cut(s) 302, 619
BstDEI CTNAG 4 cut(s) 306, 458, 666, 1176
BstENI CCTNNNNNAGG 1 cut(s) 170
BstF5I GGATG 5 cut(s) 32, 532, 673, 751, 760
BstH2I RGCGCY 1 cut(s) 283
BstHHI GCGC 2 cut(s) 13, 282
BstKTI GATC 7 cut(s) 312, 806, 880, 970, 1009, 1037, 1116
BstMAI GTCTC 2 cut(s) 68, 1154
BstMBI GATC 7 cut(s) 309, 803, 877, 967, 1006, 1034, 1113
BstMWI GCNNNNNNNGC 4 cut(s) 196, 380, 473, 614
BstNI CCWGG 2 cut(s) 173, 254
BstSCI CCNGG 4 cut(s) 171, 252, 984, 1027
BstSFI CTRYAG 2 cut(s) 295, 840
BstSLI GKGCMC 1 cut(s) 790
BstV1I GCAGC 5 cut(s) 25, 529, 592, 717, 826
BstX2I RGATCY 3 cut(s) 803, 967, 1006
BstYI RGATCY 3 cut(s) 803, 967, 1006
Bsu15I ATCGAT 1 cut(s) 1116
Bsu36I CCTNAGG 1 cut(s) 666
BsuRI GGCC 4 cut(s) 243, 329, 341, 788
BsuTUI ATCGAT 1 cut(s) 1116
BtsCI GGATG 5 cut(s) 32, 532, 673, 751, 760
BtsI GCAGTG 1 cut(s) 199
BtsIMutI CAGTG 4 cut(s) 58, 199, 451, 687
Cac8I GCNNGC 2 cut(s) 302, 619
CfoI GCGC 2 cut(s) 13, 282
Cfr13I GGNCC 6 cut(s) 175, 241, 250, 786, 787, 994
ClaI ATCGAT 1 cut(s) 1116
Csp6I GTAC 1 cut(s) 859
CviAII CATG 2 cut(s) 262, 1126
CviQI GTAC 1 cut(s) 859
DdeI CTNAG 4 cut(s) 306, 458, 666, 1176
DpnI GATC 7 cut(s) 311, 805, 879, 969, 1008, 1036, 1115
DpnII GATC 7 cut(s) 309, 803, 877, 967, 1006, 1034, 1113
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
Ecl136II GAGCTC 1 cut(s) 457
Eco130I CCWWGG 1 cut(s) 342
Eco147I AGGCCT 2 cut(s) 329, 341
Eco24I GRGCYC 2 cut(s) 459, 790
Eco47I GGWCC 3 cut(s) 175, 250, 994
Eco53kI GAGCTC 1 cut(s) 457
Eco81I CCTNAGG 1 cut(s) 666
EcoICRI GAGCTC 1 cut(s) 457
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoO109I RGGNCCY 3 cut(s) 175, 241, 786
EcoRI GAATTC 1 cut(s) 700
EcoRII CCWGG 2 cut(s) 171, 252
EcoT14I CCWWGG 1 cut(s) 342
EcoT38I GRGCYC 2 cut(s) 459, 790
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 2 cut(s) 265, 1129
FaiI YATR 9 cut(s) 263, 333, 372, 681, 827, 851, 939, 1127, 1155
FalI AAGNNNNNCTT 4 cut(s) 188, 220, 326, 358
FaqI GGGAC 1 cut(s) 1128
FatI CATG 2 cut(s) 261, 1125
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 7 cut(s) 6, 9, 14, 543, 606, 731, 840
FokI GGATG 5 cut(s) 19, 539, 660, 747, 758
FriOI GRGCYC 2 cut(s) 459, 790
Fsp4HI GCNGC 7 cut(s) 6, 9, 14, 543, 606, 731, 840
FspBI CTAG 1 cut(s) 492
FspI TGCGCA 1 cut(s) 12
GlaI GCGC 2 cut(s) 12, 281
GluI GCNGC 7 cut(s) 6, 9, 14, 543, 606, 731, 840
GsaI CCCAGC 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 283
HaeIII GGCC 4 cut(s) 243, 329, 341, 788
HapII CCGG 2 cut(s) 985, 1029
HhaI GCGC 2 cut(s) 13, 282
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 2 cut(s) 265, 1129
Hin6I GCGC 2 cut(s) 11, 280
HinP1I GCGC 2 cut(s) 11, 280
HincII GTYRAC 1 cut(s) 1066
HindII GTYRAC 1 cut(s) 1066
HinfI GANTC 1 cut(s) 266
HpaII CCGG 2 cut(s) 985, 1029
HphI GGTGA 3 cut(s) 304, 763, 1093
Hpy166II GTNNAC 2 cut(s) 661, 1066
Hpy188I TCNGA 3 cut(s) 220, 309, 875
Hpy188III TCNNGA 5 cut(s) 397, 548, 764, 833, 1002
Hpy8I GTNNAC 2 cut(s) 661, 1066
HpyAV CCTTC 6 cut(s) 31, 49, 112, 133, 165, 545
HpyCH4III ACNGT 4 cut(s) 62, 187, 236, 982
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 3 cut(s) 733, 842, 1046
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 380, 473, 614
HpyF3I CTNAG 4 cut(s) 306, 458, 666, 1176
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 2 cut(s) 265, 1129
HspAI GCGC 2 cut(s) 11, 280
Kzo9I GATC 7 cut(s) 309, 803, 877, 967, 1006, 1034, 1113
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 1 cut(s) 406
Lsp1109I GCAGC 5 cut(s) 25, 529, 592, 717, 826
LweI GCATC 2 cut(s) 826, 1147
MaeI CTAG 1 cut(s) 492
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 2 cut(s) 692, 1072
MalI GATC 7 cut(s) 311, 805, 879, 969, 1008, 1036, 1115
MboI GATC 7 cut(s) 309, 803, 877, 967, 1006, 1034, 1113
MboII GAAGA 4 cut(s) 461, 766, 944, 1044
MflI RGATCY 3 cut(s) 803, 967, 1006
MhlI GDGCHC 4 cut(s) 144, 441, 459, 790
MluCI AATT 8 cut(s) 518, 612, 645, 700, 707, 895, 1048, 1198
MmeI TCCRAC 1 cut(s) 980
MroXI GAANNNNTTC 1 cut(s) 1137
MseI TTAA 9 cut(s) 117, 195, 353, 378, 648, 894, 902, 975, 1201
MslI CAYNNNNRTG 3 cut(s) 678, 765, 942
MspI CCGG 2 cut(s) 985, 1029
MspR9I CCNGG 4 cut(s) 173, 254, 986, 1029
MvaI CCWGG 2 cut(s) 173, 254
MwoI GCNNNNNNNGC 4 cut(s) 196, 380, 473, 614
NciI CCSGG 2 cut(s) 986, 1029
NdeII GATC 7 cut(s) 309, 803, 877, 967, 1006, 1034, 1113
NlaIII CATG 2 cut(s) 265, 1129
NlaIV GGNNCC 6 cut(s) 177, 189, 787, 788, 805, 1096
NsbI TGCGCA 1 cut(s) 12
NspV TTCGAA 1 cut(s) 643
PceI AGGCCT 2 cut(s) 329, 341
PciSI GCTCTTC 1 cut(s) 474
PdmI GAANNNNTTC 1 cut(s) 1137
PfeI GAWTC 1 cut(s) 266
PfoI TCCNGGA 2 cut(s) 252, 984
PkrI GCNGC 7 cut(s) 7, 10, 15, 544, 607, 732, 841
PpuMI RGGWCCY 1 cut(s) 175
PshBI ATTAAT 1 cut(s) 975
Psp124BI GAGCTC 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 2 cut(s) 171, 252
PspFI CCCAGC 1 cut(s) 476
PspGI CCWGG 2 cut(s) 171, 252
PspN4I GGNNCC 6 cut(s) 177, 189, 787, 788, 805, 1096
PspOMI GGGCCC 1 cut(s) 786
PspPI GGNCC 6 cut(s) 175, 241, 250, 786, 787, 994
PspPPI RGGWCCY 1 cut(s) 175
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 3 cut(s) 803, 967, 1006
RsaI GTAC 1 cut(s) 860
RsaNI GTAC 1 cut(s) 859
RseI CAYNNNNRTG 3 cut(s) 678, 765, 942
SacI GAGCTC 1 cut(s) 459
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 9 cut(s) 117, 195, 353, 378, 648, 894, 902, 975, 1201
SatI GCNGC 7 cut(s) 6, 9, 14, 543, 606, 731, 840
Sau3AI GATC 7 cut(s) 309, 803, 877, 967, 1006, 1034, 1113
Sau96I GGNCC 6 cut(s) 175, 241, 250, 786, 787, 994
ScrFI CCNGG 4 cut(s) 173, 254, 986, 1029
SduI GDGCHC 4 cut(s) 144, 441, 459, 790
SfaNI GCATC 2 cut(s) 826, 1147
SfcI CTRYAG 2 cut(s) 295, 840
SfuI TTCGAA 1 cut(s) 643
SinI GGWCC 3 cut(s) 175, 250, 994
SmiMI CAYNNNNRTG 3 cut(s) 678, 765, 942
SmlI CTYRAG 2 cut(s) 245, 546
SmoI CTYRAG 2 cut(s) 245, 546
Sse9I AATT 8 cut(s) 518, 612, 645, 700, 707, 895, 1048, 1198
SseBI AGGCCT 2 cut(s) 329, 341
SsiI CCGC 1 cut(s) 5
SspI AATATT 2 cut(s) 595, 634
SspMI CTAG 1 cut(s) 492
SstI GAGCTC 1 cut(s) 459
StuI AGGCCT 2 cut(s) 329, 341
StyD4I CCNGG 4 cut(s) 171, 252, 984, 1027
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 4 cut(s) 62, 187, 236, 982
TaiI ACGT 1 cut(s) 261
TaqI TCGA 7 cut(s) 472, 570, 582, 643, 832, 855, 1116
TasI AATT 8 cut(s) 518, 612, 645, 700, 707, 895, 1048, 1198
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 9 cut(s) 117, 195, 353, 378, 648, 894, 902, 975, 1201
Tru9I TTAA 9 cut(s) 117, 195, 353, 378, 648, 894, 902, 975, 1201
TscAI CASTG 4 cut(s) 65, 206, 451, 694
TseI GCWGC 6 cut(s) 8, 13, 542, 605, 730, 839
TspDTI ATGAA 1 cut(s) 662
TspRI CASTG 4 cut(s) 65, 206, 451, 694
VpaK11BI GGWCC 3 cut(s) 175, 250, 994
VspI ATTAAT 1 cut(s) 975
XagI CCTNNNNNAGG 1 cut(s) 170
XapI RAATTY 4 cut(s) 518, 700, 707, 1048
XcmI CCANNNNNNNNNTGG 2 cut(s) 797, 817
XmiI GTMKAC 1 cut(s) 660
XmnI GAANNNNTTC 1 cut(s) 1137
XspI CTAG 1 cut(s) 492
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.