FvH4_2g12940

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016756
Physical Location & Seq
Reverse (-)
11349470 .. 11351690
2221 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g12940.t1

Sequence Viewer

Length: 1389 bp
ATGAAATCCTCATCTCATTTCAAATCCCTCCAAGCTTCTATTTTGGGAGTCTTAGTAATCTTAAGTCTCCTTCACTTTCTCTTCTCATGTTCCACCCTCTCTTTGTCCTGCATTTGGCTTCTTTGCATTGCTAGTCTTTACTACTATTCCTCTCGTCCCAACCCCGTCTTCCTCCTTGACTTCTCCTGCTTCAAACCAGACCCTAACCAGAAATGTAACGTTCACTCATCCGAATCCTTCGTGCTCGGCACGAATCGCTTCTCCGAGGAAAGCATAGGATTCATGCGTAAAATCTTCCTCAAATCCGGCCTAGGAGAAGAAACCTATGCGCCAAAGTTCGTTTTCCAATCCAACCCCGAAGCCAAGTTAGAGTCCGCCATGGATGAAGCTCAGCAAGGCATGTTCTCAGCCGTGAGCTCACTCCTTTCCAAAACCGGCATCCAGCCGTCTCTCATCAACGCCCTGATCGTGACATGTGGCAGCTTCTCTCCGGCGCCCTCTCTCTCGTCGCTGATGGTCAACCGTTTTCAGCTCAGACCCGACGTAAAAACCTACAATCTAAGCGGCATGGGGTGTAGCTCTGGTGTGATATCAATCGATTTAGCTGCTAACTTACTAAAGAAGAGCAAAGAGATTGGATACGTCTTGGTGGTCATTACCGAGAGTATTAGTTTGAACTGGTATTTTGGTGATAGTCGTCCCATGCTGGTAACCAACTGCATTTTTAGGGTTGGTTGTGCTGCGGCGTTGATTACAAATGATCCGAGTTGTCGCCGTGTCGCCAAGATGGAACTCATACAGTCTCTTCGAACTCACCACGGAGCAAGCGACCGAGCATATAAAGCTGCATTTCAAGAGGAAGATGATAAGGGCAACCTAGGTTTTGCTCTGACTAAAGATTTGATTCCAGTCGCAGGGAACTATTTACGTGAGCACATCAAGATTCTGGCTCCCCGAGTTTTACCATTGAATCAACTTGCTATGTACGCCTACTCGGTCCTTTGCTCTGCAGCAACATGGGGGAACAAAAGCAAGTTCAAACCACTATTTGTCCCAGATTTCACCAAAGCTTTTGACCATTTTTGTATCCATACCGGTGGGAAAGCTGTGATCGAGCAGGTGGGGAGGGTTCTGCGATTGGGAGACATGTTGACTGAGCCGGCTCGGATGAGTTTGCACCGATTTGGGAACACTTCGAGTAGTCTAGTGTTCTATGAGTTGGCTTATTTTGAAGCCAAACGGAGAATTAAGGAAGGAGATAGGGTGTGGATGTTGGCGTTTGGGACTGGGTTTAAGGTAGGGAGTCTTGTTTGGAAGTCACTTTCCGATTTAGGTCAACAGAGTGATAATCCATGGAGTGACTGCATTGATAACTATCCATTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

463

Amino Acids

51.23

Weight (kDa)

8.91

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 49 - 335 2.5e-107 FAE1/Type III polyketide synthase-like protein
Chal_sti_synt_C PF02797 351 - 441 1.8e-07 Chalcone and stilbene synthases, C-terminal domain
ACP_syn_III_C PF08541 358 - 439 2.5e-10 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016756)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12940
malus_domestica MD10G1036400.v1.1
prunus_persica Prupe.8G042200_v2.0.a1
pyrus_communis pycom05g02490 pycom10g02490
rosa_chinensis RchiOBHm_Chr6g0274011
rosa_laevigata RLG00000013584
rosa_multiflora Rmu_sc0002247.1_g000012
rosa_rugosa Rorug06G0078400
rosa_samantha Rh6AG193900 Rh6BG197500 Rh6CG197600 Rh6DG189300
rosa_wichuraiana Rw6G016910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1108
Acc36I ACCTGC 1 cut(s) 1108
AccB1I GGYRCC 1 cut(s) 493
AciI CCGC 3 cut(s) 375, 564, 743
AclI AACGTT 1 cut(s) 219
AclWI GGATC 1 cut(s) 755
AcyI GRCGYC 1 cut(s) 494
AfaI GTAC 1 cut(s) 986
AfiI CCNNNNNNNGG 2 cut(s) 114, 914
AflII CTTAAG 1 cut(s) 61
AflIII ACRYGT 2 cut(s) 473, 1146
AgeI ACCGGT 1 cut(s) 1094
AgsI TTSAA 7 cut(s) 22, 193, 676, 854, 970, 1039, 1232
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
Alw21I GWGCWC 3 cut(s) 246, 419, 936
Alw26I GTCTC 4 cut(s) 71, 453, 807, 1137
AlwI GGATC 1 cut(s) 755
Ama87I CYCGRG 1 cut(s) 954
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 5 cut(s) 480, 605, 740, 845, 1010
ArsI GACNNNNNNTTYG 2 cut(s) 883, 915
AsiGI ACCGGT 1 cut(s) 1094
Asp700I GAANNNNTTC 1 cut(s) 257
AspA2I CCTAGG 2 cut(s) 310, 877
AspLEI GCGC 2 cut(s) 331, 496
AspS9I GGNCC 1 cut(s) 997
AsuHPI GGTGA 3 cut(s) 701, 806, 1054
AsuII TTCGAA 1 cut(s) 808
AvaI CYCGRG 1 cut(s) 954
AvaII GGWCC 1 cut(s) 997
AvrII CCTAGG 2 cut(s) 310, 877
BanI GGYRCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 419
BbsI GAAGAC 1 cut(s) 160
Bbv12I GWGCWC 3 cut(s) 246, 419, 936
BbvI GCAGC 5 cut(s) 492, 592, 727, 832, 1022
BccI CCATC 2 cut(s) 508, 781
BceAI ACGGC 3 cut(s) 395, 430, 759
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciVI GTATCC 2 cut(s) 632, 1097
BcoDI GTCTC 4 cut(s) 71, 453, 807, 1137
BfaI CTAG 4 cut(s) 132, 311, 878, 1205
BfmI CTRYAG 1 cut(s) 1008
BfoI RGCGCY 1 cut(s) 497
BfrI CTTAAG 1 cut(s) 61
BfuAI ACCTGC 1 cut(s) 1108
BfuI GTATCC 2 cut(s) 632, 1097
BisI GCNGC 7 cut(s) 481, 565, 606, 741, 744, 846, 1011
BlnI CCTAGG 2 cut(s) 310, 877
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 7 cut(s) 482, 566, 607, 742, 745, 847, 1012
Bme18I GGWCC 1 cut(s) 997
BmeT110I CYCGRG 1 cut(s) 954
BmgT120I GGNCC 1 cut(s) 997
BmiI GGNNCC 2 cut(s) 495, 951
BmrI ACTGGG 1 cut(s) 1296
BmsI GCATC 1 cut(s) 447
BmuI ACTGGG 1 cut(s) 1296
BpiI GAAGAC 1 cut(s) 160
Bpu1102I GCTNAGC 1 cut(s) 390
Bpu14I TTCGAA 1 cut(s) 808
Bsa29I ATCGAT 1 cut(s) 597
BsaAI YACGTR 1 cut(s) 929
BsaBI GATNNNNATC 2 cut(s) 593, 1374
BsaHI GRCGYC 1 cut(s) 494
BsaJI CCNNGG 6 cut(s) 264, 310, 378, 817, 877, 1352
BsaWI WCCGGW 1 cut(s) 1094
BsaXI ACNNNNNCTCC 4 cut(s) 39, 69, 1248, 1278
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 914
Bse118I RCCGGY 3 cut(s) 434, 1094, 1159
Bse1I ACTGG 3 cut(s) 683, 908, 1291
Bse3DI GCAATG 1 cut(s) 126
Bse8I GATNNNNATC 2 cut(s) 593, 1374
BseCI ATCGAT 1 cut(s) 597
BseDI CCNNGG 6 cut(s) 264, 310, 378, 817, 877, 1352
BseGI GGATG 5 cut(s) 227, 388, 438, 1173, 1275
BseJI GATNNNNATC 2 cut(s) 593, 1374
BseLI CCNNNNNNNGG 2 cut(s) 114, 914
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 4 cut(s) 404, 420, 547, 1146
BseNI ACTGG 3 cut(s) 683, 908, 1291
BseXI GCAGC 5 cut(s) 492, 592, 727, 832, 1022
Bsh1285I CGRYCG 1 cut(s) 832
BshFI GGCC 1 cut(s) 309
BshNI GGYRCC 1 cut(s) 493
BshTI ACCGGT 1 cut(s) 1094
BshVI ATCGAT 1 cut(s) 597
BsiEI CGRYCG 1 cut(s) 832
BsiHKAI GWGCWC 3 cut(s) 246, 419, 936
BsiHKCI CYCGRG 1 cut(s) 954
BsiSI CCGG 5 cut(s) 306, 435, 491, 1095, 1160
BslFI GGGAC 4 cut(s) 141, 684, 1037, 1297
BslI CCNNNNNNNGG 2 cut(s) 114, 914
BsmAI GTCTC 4 cut(s) 71, 453, 807, 1137
BsmBI CGTCTC 1 cut(s) 453
BsmFI GGGAC 4 cut(s) 141, 684, 1037, 1297
BsnI GGCC 1 cut(s) 309
BsoBI CYCGRG 1 cut(s) 954
Bsp119I TTCGAA 1 cut(s) 808
Bsp1286I GDGCHC 3 cut(s) 246, 419, 936
Bsp143I GATC 3 cut(s) 465, 760, 1110
Bsp1720I GCTNAGC 1 cut(s) 390
Bsp19I CCATGG 2 cut(s) 378, 1352
BspACI CCGC 3 cut(s) 375, 564, 743
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 4 cut(s) 403, 419, 546, 1147
BspDI ATCGAT 1 cut(s) 597
BspLI GGNNCC 2 cut(s) 495, 951
BspMAI CTGCAG 1 cut(s) 1012
BspMI ACCTGC 1 cut(s) 1108
BspPI GGATC 1 cut(s) 755
BspQI GCTCTTC 1 cut(s) 617
BspT104I TTCGAA 1 cut(s) 808
BspT107I GGYRCC 1 cut(s) 493
BspTI CTTAAG 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 126
BsrFI RCCGGY 3 cut(s) 434, 1094, 1159
BsrI ACTGG 3 cut(s) 683, 908, 1291
BssAI RCCGGY 3 cut(s) 434, 1094, 1159
BssECI CCNNGG 6 cut(s) 264, 310, 378, 817, 877, 1352
BssMI GATC 3 cut(s) 465, 760, 1110
BssNI GRCGYC 1 cut(s) 494
BssT1I CCWWGG 4 cut(s) 310, 378, 877, 1352
Bst4CI ACNGT 2 cut(s) 524, 801
Bst6I CTCTTC 3 cut(s) 86, 617, 810
BstACI GRCGYC 1 cut(s) 494
BstAFI CTTAAG 1 cut(s) 61
BstBAI YACGTR 1 cut(s) 929
BstBI TTCGAA 1 cut(s) 808
BstC8I GCNNGC 2 cut(s) 826, 1161
BstDEI CTNAG 6 cut(s) 52, 390, 406, 533, 560, 1155
BstDSI CCRYGG 3 cut(s) 378, 817, 1352
BstEII GGTNACC 1 cut(s) 709
BstF5I GGATG 5 cut(s) 227, 388, 438, 1173, 1275
BstH2I RGCGCY 1 cut(s) 497
BstHHI GCGC 2 cut(s) 331, 496
BstKTI GATC 3 cut(s) 468, 763, 1113
BstMAI GTCTC 4 cut(s) 71, 453, 807, 1137
BstMBI GATC 3 cut(s) 465, 760, 1110
BstMCI CGRYCG 1 cut(s) 832
BstMWI GCNNNNNNNGC 3 cut(s) 255, 842, 986
BstNSI RCATGY 3 cut(s) 403, 477, 1150
BstPI GGTNACC 1 cut(s) 709
BstSFI CTRYAG 1 cut(s) 1008
BstV1I GCAGC 5 cut(s) 492, 592, 727, 832, 1022
BstV2I GAAGAC 1 cut(s) 160
BstXI CCANNNNNNTGG 1 cut(s) 1097
Bsu15I ATCGAT 1 cut(s) 597
BsuI GTATCC 2 cut(s) 632, 1097
BsuRI GGCC 1 cut(s) 309
BsuTUI ATCGAT 1 cut(s) 597
BtgI CCRYGG 3 cut(s) 378, 817, 1352
BtsCI GGATG 5 cut(s) 227, 388, 438, 1173, 1275
BveI ACCTGC 1 cut(s) 1108
Cac8I GCNNGC 2 cut(s) 826, 1161
CfoI GCGC 2 cut(s) 331, 496
Cfr10I RCCGGY 3 cut(s) 434, 1094, 1159
Cfr13I GGNCC 1 cut(s) 997
ClaI ATCGAT 1 cut(s) 597
Csp6I GTAC 1 cut(s) 985
CspAI ACCGGT 1 cut(s) 1094
CviQI GTAC 1 cut(s) 985
DdeI CTNAG 6 cut(s) 52, 390, 406, 533, 560, 1155
DinI GGCGCC 1 cut(s) 495
DpnI GATC 3 cut(s) 467, 762, 1112
DpnII GATC 3 cut(s) 465, 760, 1110
Eam1104I CTCTTC 3 cut(s) 86, 617, 810
EarI CTCTTC 3 cut(s) 86, 617, 810
EciI GGCGGA 1 cut(s) 364
Ecl136II GAGCTC 1 cut(s) 417
Eco130I CCWWGG 4 cut(s) 310, 378, 877, 1352
Eco24I GRGCYC 1 cut(s) 419
Eco32I GATATC 1 cut(s) 591
Eco47I GGWCC 1 cut(s) 997
Eco53kI GAGCTC 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 954
Eco91I GGTNACC 1 cut(s) 709
EcoICRI GAGCTC 1 cut(s) 417
EcoO65I GGTNACC 1 cut(s) 709
EcoRV GATATC 1 cut(s) 591
EcoT14I CCWWGG 4 cut(s) 310, 378, 877, 1352
EcoT38I GRGCYC 1 cut(s) 419
EgeI GGCGCC 1 cut(s) 495
EheI GGCGCC 1 cut(s) 495
ErhI CCWWGG 4 cut(s) 310, 378, 877, 1352
Esp3I CGTCTC 1 cut(s) 453
FaqI GGGAC 4 cut(s) 141, 684, 1037, 1297
Fnu4HI GCNGC 7 cut(s) 481, 565, 606, 741, 744, 846, 1011
FokI GGATG 5 cut(s) 214, 395, 425, 1180, 1282
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 7 cut(s) 481, 565, 606, 741, 744, 846, 1011
FspBI CTAG 4 cut(s) 132, 311, 878, 1205
GlaI GCGC 2 cut(s) 330, 495
GluI GCNGC 7 cut(s) 481, 565, 606, 741, 744, 846, 1011
HaeII RGCGCY 1 cut(s) 497
HaeIII GGCC 1 cut(s) 309
HapII CCGG 5 cut(s) 306, 435, 491, 1095, 1160
HhaI GCGC 2 cut(s) 331, 496
Hin1I GRCGYC 1 cut(s) 494
Hin6I GCGC 2 cut(s) 329, 494
HinP1I GCGC 2 cut(s) 329, 494
HincII GTYRAC 3 cut(s) 520, 1152, 1337
HindII GTYRAC 3 cut(s) 520, 1152, 1337
HindIII AAGCTT 2 cut(s) 33, 1068
HinfI GANTC 9 cut(s) 48, 233, 253, 279, 371, 904, 943, 970, 1303
HpaII CCGG 5 cut(s) 306, 435, 491, 1095, 1160
HphI GGTGA 3 cut(s) 701, 806, 1054
Hpy166II GTNNAC 4 cut(s) 223, 520, 1152, 1337
Hpy188I TCNGA 7 cut(s) 232, 265, 536, 765, 891, 1167, 1327
Hpy188III TCNNGA 3 cut(s) 469, 854, 940
Hpy8I GTNNAC 4 cut(s) 223, 520, 1152, 1337
Hpy99I CGWCG 2 cut(s) 511, 545
HpyAV CCTTC 3 cut(s) 80, 247, 1247
HpyCH4III ACNGT 2 cut(s) 524, 801
HpyCH4IV ACGT 4 cut(s) 219, 543, 642, 928
HpyCH4V TGCA 7 cut(s) 111, 126, 720, 848, 1010, 1177, 1365
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 842, 986
HpyF3I CTNAG 6 cut(s) 52, 390, 406, 533, 560, 1155
HpySE526I ACGT 4 cut(s) 219, 543, 642, 928
Hsp92I GRCGYC 1 cut(s) 494
HspAI GCGC 2 cut(s) 329, 494
KasI GGCGCC 1 cut(s) 493
KroI GCCGGC 1 cut(s) 1159
KroNI GCCGGC 1 cut(s) 1161
Kzo9I GATC 3 cut(s) 465, 760, 1110
LguI GCTCTTC 1 cut(s) 617
LmnI GCTCC 2 cut(s) 821, 955
Lsp1109I GCAGC 5 cut(s) 492, 592, 727, 832, 1022
LweI GCATC 1 cut(s) 447
MaeI CTAG 4 cut(s) 132, 311, 878, 1205
MaeII ACGT 4 cut(s) 219, 543, 642, 928
MaeIII GTNAC 5 cut(s) 215, 469, 709, 1317, 1358
MalI GATC 3 cut(s) 467, 762, 1112
MboI GATC 3 cut(s) 465, 760, 1110
MboII GAAGA 7 cut(s) 73, 160, 286, 329, 634, 797, 872
MhlI GDGCHC 3 cut(s) 246, 419, 936
MluCI AATT 1 cut(s) 1245
Mly113I GGCGCC 1 cut(s) 494
MlyI GAGTC 3 cut(s) 57, 380, 1312
MmeI TCCRAC 1 cut(s) 375
MroNI GCCGGC 1 cut(s) 1159
MroXI GAANNNNTTC 1 cut(s) 257
MseI TTAA 4 cut(s) 62, 1248, 1293, 1382
MslI CAYNNNNRTG 2 cut(s) 1095, 1384
MspCI CTTAAG 1 cut(s) 61
MspI CCGG 5 cut(s) 306, 435, 491, 1095, 1160
MwoI GCNNNNNNNGC 3 cut(s) 255, 842, 986
NaeI GCCGGC 1 cut(s) 1161
NarI GGCGCC 1 cut(s) 494
NcoI CCATGG 2 cut(s) 378, 1352
NdeII GATC 3 cut(s) 465, 760, 1110
NgoMIV GCCGGC 1 cut(s) 1159
NlaIV GGNNCC 2 cut(s) 495, 951
NmeAIII GCCGAG 1 cut(s) 225
NmuCI GTSAC 3 cut(s) 469, 1317, 1358
NspI RCATGY 3 cut(s) 403, 477, 1150
NspV TTCGAA 1 cut(s) 808
PaqCI CACCTGC 1 cut(s) 1108
PciI ACATGT 2 cut(s) 473, 1146
PciSI GCTCTTC 1 cut(s) 617
PcsI WCGNNNNNNNCGW 2 cut(s) 465, 825
PdiI GCCGGC 1 cut(s) 1161
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 6 cut(s) 233, 253, 279, 904, 943, 970
PinAI ACCGGT 1 cut(s) 1094
PkrI GCNGC 7 cut(s) 482, 566, 607, 742, 745, 847, 1012
PleI GAGTC 3 cut(s) 56, 379, 1311
PluTI GGCGCC 1 cut(s) 497
PpsI GAGTC 3 cut(s) 56, 379, 1311
Ppu21I YACGTR 1 cut(s) 929
PscI ACATGT 2 cut(s) 473, 1146
Psp124BI GAGCTC 1 cut(s) 419
Psp1406I AACGTT 1 cut(s) 219
PspEI GGTNACC 1 cut(s) 709
PspN4I GGNNCC 2 cut(s) 495, 951
PspPI GGNCC 1 cut(s) 997
PstI CTGCAG 1 cut(s) 1012
RsaI GTAC 1 cut(s) 986
RsaNI GTAC 1 cut(s) 985
RseI CAYNNNNRTG 2 cut(s) 1095, 1384
SacI GAGCTC 1 cut(s) 419
SapI GCTCTTC 1 cut(s) 617
SaqAI TTAA 4 cut(s) 62, 1248, 1293, 1382
SatI GCNGC 7 cut(s) 481, 565, 606, 741, 744, 846, 1011
Sau3AI GATC 3 cut(s) 465, 760, 1110
Sau96I GGNCC 1 cut(s) 997
SchI GAGTC 3 cut(s) 57, 380, 1312
SduI GDGCHC 3 cut(s) 246, 419, 936
SfaNI GCATC 1 cut(s) 447
SfcI CTRYAG 1 cut(s) 1008
SfoI GGCGCC 1 cut(s) 495
SfuI TTCGAA 1 cut(s) 808
SinI GGWCC 1 cut(s) 997
SmiMI CAYNNNNRTG 2 cut(s) 1095, 1384
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
Sse9I AATT 1 cut(s) 1245
SsiI CCGC 3 cut(s) 375, 564, 743
SspDI GGCGCC 1 cut(s) 493
SspMI CTAG 4 cut(s) 132, 311, 878, 1205
SstI GAGCTC 1 cut(s) 419
StyI CCWWGG 4 cut(s) 310, 378, 877, 1352
TaaI ACNGT 2 cut(s) 524, 801
TaiI ACGT 4 cut(s) 222, 546, 645, 931
TaqI TCGA 4 cut(s) 597, 808, 1113, 1196
TaqII GACCGA 2 cut(s) 846, 985
TasI AATT 1 cut(s) 1245
TauI GCSGC 2 cut(s) 567, 746
TfiI GAWTC 6 cut(s) 233, 253, 279, 904, 943, 970
Tru1I TTAA 4 cut(s) 62, 1248, 1293, 1382
Tru9I TTAA 4 cut(s) 62, 1248, 1293, 1382
TseFI GTSAC 3 cut(s) 469, 1317, 1358
TseI GCWGC 5 cut(s) 480, 605, 740, 845, 1010
Tsp45I GTSAC 3 cut(s) 469, 1317, 1358
TspDTI ATGAA 3 cut(s) 17, 271, 399
TspGWI ACGGA 2 cut(s) 834, 1255
Vha464I CTTAAG 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 997
XceI RCATGY 3 cut(s) 403, 477, 1150
XmaJI CCTAGG 2 cut(s) 310, 877
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 4 cut(s) 132, 311, 878, 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.