MD10G1036400.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016756
Physical Location & Seq
Reverse (-)
4821741 .. 4823195
1455 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1036400.v1.1.491

Sequence Viewer

Length: 1455 bp
ATGAAGAAATCCACTTCAAATTTCAAATTCCACCAAGCCTTTTTCGTAGCACTTTTATTAACCACAACTCTCTTATCAATTAACATGCAGTCTAATTCAATCCGATCCAATCCAGTCCAATACAGTAACATCCTCCATTTTCTCTTCACCACCAACCCTACACTTTCATTGTCTTGCATTTGGTGCCTTTCGATTTCCATATTTTTCTACTACTACACCTCTCGTCCCAAGTCCATCTTCCTCCTTGATTTCTCATGCTTCCAACCAAACCCTAACCAAAAATGCAGCTACGCAGCCTCCGAGTCCTTCGTACGCAGGACGAATCGCTTCACCGAAGAAAGCGAAGAATTCATGCGTAAAATCTACCTCAAATCCGGCTTAGGAGACGAGACCTACGCACCCCCATTCGTCTTCGAATCAAACCCCGAAGCGAACTTGAAATATGCTCTAGATGAAGCTCAACAAGGCATGTTCTCATCCATCAACTCACTCCTTGCCAAAACCAACATCGACCCAAGTTGTATCGATTGCCTAATTGTTACATGCGGGAGCTTCTCTCCCATGCCCTCCCTCACATCCCTCATTGTCAACCGTTTCAAGCTCAAACCCGACGTAAAGACGTACAATCTAAGTGGAATGGGATGCAGTTCCGGAGTAATATCAATTGATCTAGCCGCCAATCTGTTGAAGCAAAGCAAAAAAATTGGTTATGCTCTTGTGGTCGTAACCGAGACTATTAGTTTGGATTGGTACTATGGCGATAACCGTCCCATGCTTGTAACCAATTGCATTTTTCGAGTCGGCTGTGCGACCGCATTGATCACAAACGACCCGAGTTGCCGCCGAGTTGCAAAGATGGAACTCGTTCACTCGCTTCGTACTCACCACGGAGCGAGTGATCGAGCTTATAAAGCCGCATTTCAAGAGGAGGATAACAAGGGCAATACCGGGTTTGCTCTTAATAAAGATTTAACAGCGGTTGCAGGCATGCATTTACTTGAGCACATCAAAATTCTTGCTCCCCGAGTCCTGCCACTGACTCAACTTGGTGTTTACGTGTACTCGGTCATTGGTTGTGCATTTTCAGGGGGGAAGTCGAAACCGATAGTCCCGGATTTCACCAAGGCATTTGATCATTTTTGTATTCACACGGGTGGGAAAGCGGTGATCGAGCAGGTTGGGCGGGTTCTGAGTTTGAGTGACGCGTTGACTGAGCCGGCTCGGATGAGTTTGCACCGATTCGGGAACACGTCGAGTAGTCTTGTATTTTATGAGTTAGCATATTTTGAGGCTAAAGGGAGAGTTAGGAAAGGAGATAGGGTTTGGATGTTGGCGTTTGGGACAGGGTTTAAGGTCGGAAGTCTTGTTTGGAAGGCAGTTTCGGATTTAGGGCAAGGTGGTGATGACAATCCATGGAGCGACTGTATTGATAGGTACCCATTGAAGTCATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

485

Amino Acids

53.91

Weight (kDa)

8.86

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 72 - 356 3.9e-107 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 377 - 458 4.9e-11 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 380 - 460 4e-07 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016756)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12940
malus_domestica MD10G1036400.v1.1
prunus_persica Prupe.8G042200_v2.0.a1
pyrus_communis pycom05g02490 pycom10g02490
rosa_chinensis RchiOBHm_Chr6g0274011
rosa_laevigata RLG00000013584
rosa_multiflora Rmu_sc0002247.1_g000012
rosa_rugosa Rorug06G0078400
rosa_samantha Rh6AG193900 Rh6BG197500 Rh6CG197600 Rh6DG189300
rosa_wichuraiana Rw6G016910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 909
Acc36I ACCTGC 1 cut(s) 1165
Acc65I GGTACC 1 cut(s) 1434
AccB1I GGYRCC 2 cut(s) 183, 1434
AccII CGCG 1 cut(s) 1205
AccIII TCCGGA 1 cut(s) 650
AciI CCGC 8 cut(s) 546, 675, 813, 841, 915, 977, 1163, 1183
AclWI GGATC 1 cut(s) 99
AcsI RAATTY 4 cut(s) 19, 26, 347, 1011
AfaI GTAC 6 cut(s) 312, 623, 752, 880, 1061, 1436
AflIII ACRYGT 3 cut(s) 1056, 1203, 1248
AgsI TTSAA 8 cut(s) 18, 25, 99, 439, 598, 688, 923, 1444
AjiI CACGTC 1 cut(s) 1251
AjuI GAANNNNNNNTTGG 4 cut(s) 221, 253, 1351, 1383
AleI CACNNNNGTG 1 cut(s) 1152
AluBI AGCT 5 cut(s) 288, 458, 552, 601, 905
AluI AGCT 5 cut(s) 288, 458, 552, 601, 905
Alw21I GWGCWC 1 cut(s) 1005
Alw26I GTCTC 3 cut(s) 378, 383, 725
AlwI GGATC 1 cut(s) 99
Ama87I CYCGRG 2 cut(s) 832, 1023
Aor13HI TCCGGA 1 cut(s) 650
ApeKI GCWGC 2 cut(s) 285, 293
ApoI RAATTY 4 cut(s) 19, 26, 347, 1011
ArsI GACNNNNNNTTYG 2 cut(s) 724, 756
Asp700I GAANNNNTTC 2 cut(s) 326, 864
Asp718I GGTACC 1 cut(s) 1434
AsuC2I CCSGG 2 cut(s) 949, 1112
AsuHPI GGTGA 6 cut(s) 139, 322, 875, 1111, 1177, 1412
AsuII TTCGAA 1 cut(s) 414
AvaI CYCGRG 2 cut(s) 832, 1023
BanI GGYRCC 2 cut(s) 183, 1434
BbsI GAAGAC 1 cut(s) 403
Bbv12I GWGCWC 1 cut(s) 1005
BbvI GCAGC 2 cut(s) 297, 305
BccI CCATC 3 cut(s) 242, 488, 850
BclI TGATCA 2 cut(s) 819, 1132
BcnI CCSGG 2 cut(s) 949, 1112
BcoDI GTCTC 3 cut(s) 378, 383, 725
BfaI CTAG 2 cut(s) 449, 671
BfuAI ACCTGC 1 cut(s) 1165
BisI GCNGC 5 cut(s) 286, 294, 675, 841, 915
BlsI GCNGC 5 cut(s) 287, 295, 676, 842, 916
Bme1390I CCNGG 2 cut(s) 949, 1112
BmeT110I CYCGRG 2 cut(s) 832, 1023
BmgBI CACGTC 1 cut(s) 1251
BmiI GGNNCC 2 cut(s) 185, 1436
BmrFI CCNGG 2 cut(s) 949, 1112
BmsI GCATC 1 cut(s) 632
BpiI GAAGAC 1 cut(s) 403
BplI GAGNNNNNCTC 2 cut(s) 541, 573
Bpu10I CCTNAGC 1 cut(s) 379
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 1019
BpuMI CCSGG 2 cut(s) 949, 1112
Bsa29I ATCGAT 1 cut(s) 525
BsaAI YACGTR 1 cut(s) 1057
BsaBI GATNNNNATC 1 cut(s) 1407
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 886, 1122, 1412
BsaWI WCCGGW 1 cut(s) 650
BsaXI ACNNNNNCTCC 6 cut(s) 281, 311, 1305, 1335, 1408, 1438
Bse118I RCCGGY 1 cut(s) 1216
Bse1I ACTGG 1 cut(s) 113
Bse8I GATNNNNATC 1 cut(s) 1407
BseAI TCCGGA 1 cut(s) 650
BseCI ATCGAT 1 cut(s) 525
BseDI CCNNGG 3 cut(s) 886, 1122, 1412
BseGI GGATG 6 cut(s) 129, 476, 575, 647, 1230, 1332
BseJI GATNNNNATC 1 cut(s) 1407
BseMII CTCAG 2 cut(s) 1181, 1203
BseNI ACTGG 1 cut(s) 113
BseRI GAGGAG 1 cut(s) 941
BseXI GCAGC 2 cut(s) 297, 305
Bsh1236I CGCG 1 cut(s) 1205
Bsh1285I CGRYCG 1 cut(s) 813
BshNI GGYRCC 2 cut(s) 183, 1434
BshVI ATCGAT 1 cut(s) 525
BsiEI CGRYCG 1 cut(s) 813
BsiHKAI GWGCWC 1 cut(s) 1005
BsiHKCI CYCGRG 2 cut(s) 832, 1023
BsiSI CCGG 5 cut(s) 375, 651, 948, 1112, 1217
BsiWI CGTACG 1 cut(s) 310
BslFI GGGAC 4 cut(s) 210, 753, 1094, 1354
BsmAI GTCTC 3 cut(s) 378, 383, 725
BsmBI CGTCTC 1 cut(s) 378
BsmFI GGGAC 4 cut(s) 210, 753, 1094, 1354
Bso31I GGTCTC 1 cut(s) 383
BsoBI CYCGRG 2 cut(s) 832, 1023
Bsp119I TTCGAA 1 cut(s) 414
Bsp1286I GDGCHC 1 cut(s) 1005
Bsp13I TCCGGA 1 cut(s) 650
Bsp143I GATC 6 cut(s) 104, 667, 819, 898, 1132, 1167
Bsp19I CCATGG 1 cut(s) 1412
BspACI CCGC 8 cut(s) 546, 675, 813, 841, 915, 977, 1163, 1183
BspCNI CTCAG 2 cut(s) 1182, 1204
BspDI ATCGAT 1 cut(s) 525
BspEI TCCGGA 1 cut(s) 650
BspFNI CGCG 1 cut(s) 1205
BspLI GGNNCC 2 cut(s) 185, 1436
BspMI ACCTGC 1 cut(s) 1165
BspPI GGATC 1 cut(s) 99
BspT104I TTCGAA 1 cut(s) 414
BspT107I GGYRCC 2 cut(s) 183, 1434
BspTNI GGTCTC 1 cut(s) 383
BsrFI RCCGGY 1 cut(s) 1216
BsrI ACTGG 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 1216
BssECI CCNNGG 3 cut(s) 886, 1122, 1412
BssMI GATC 6 cut(s) 104, 667, 819, 898, 1132, 1167
BssT1I CCWWGG 2 cut(s) 1122, 1412
Bst4CI ACNGT 4 cut(s) 125, 593, 767, 1424
Bst6I CTCTTC 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 183
BstBAI YACGTR 1 cut(s) 1057
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 985, 989, 1218
BstDEI CTNAG 4 cut(s) 379, 629, 1190, 1212
BstDSI CCRYGG 2 cut(s) 886, 1412
BstF5I GGATG 6 cut(s) 129, 476, 575, 647, 1230, 1332
BstFNI CGCG 1 cut(s) 1205
BstKTI GATC 6 cut(s) 107, 670, 822, 901, 1135, 1170
BstMAI GTCTC 3 cut(s) 378, 383, 725
BstMBI GATC 6 cut(s) 104, 667, 819, 898, 1132, 1167
BstMCI CGRYCG 1 cut(s) 813
BstMWI GCNNNNNNNGC 3 cut(s) 183, 911, 1180
BstNSI RCATGY 4 cut(s) 88, 472, 546, 991
BstSCI CCNGG 2 cut(s) 947, 1110
BstUI CGCG 1 cut(s) 1205
BstV1I GCAGC 2 cut(s) 297, 305
BstV2I GAAGAC 1 cut(s) 403
Bsu15I ATCGAT 1 cut(s) 525
BsuTUI ATCGAT 1 cut(s) 525
BtgI CCRYGG 2 cut(s) 886, 1412
BtrI CACGTC 1 cut(s) 1251
BtsCI GGATG 6 cut(s) 129, 476, 575, 647, 1230, 1332
BtsIMutI CAGTG 1 cut(s) 1034
BveI ACCTGC 1 cut(s) 1165
Cac8I GCNNGC 3 cut(s) 985, 989, 1218
Cfr10I RCCGGY 1 cut(s) 1216
ClaI ATCGAT 1 cut(s) 525
CseI GACGC 1 cut(s) 1211
Csp6I GTAC 6 cut(s) 311, 622, 751, 879, 1060, 1435
CspCI CAANNNNNGTGG 2 cut(s) 613, 648
CviQI GTAC 6 cut(s) 311, 622, 751, 879, 1060, 1435
DdeI CTNAG 4 cut(s) 379, 629, 1190, 1212
DpnI GATC 6 cut(s) 106, 669, 821, 900, 1134, 1169
DpnII GATC 6 cut(s) 104, 667, 819, 898, 1132, 1167
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Eco130I CCWWGG 2 cut(s) 1122, 1412
Eco31I GGTCTC 1 cut(s) 383
Eco88I CYCGRG 2 cut(s) 832, 1023
EcoRI GAATTC 1 cut(s) 347
EcoT14I CCWWGG 2 cut(s) 1122, 1412
EcoT22I ATGCAT 1 cut(s) 993
ErhI CCWWGG 2 cut(s) 1122, 1412
Esp3I CGTCTC 1 cut(s) 378
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FaqI GGGAC 4 cut(s) 210, 753, 1094, 1354
FauI CCCGC 2 cut(s) 539, 1176
FbaI TGATCA 2 cut(s) 819, 1132
Fnu4HI GCNGC 5 cut(s) 286, 294, 675, 841, 915
FokI GGATG 6 cut(s) 116, 463, 562, 654, 1237, 1339
Fsp4HI GCNGC 5 cut(s) 286, 294, 675, 841, 915
FspBI CTAG 2 cut(s) 449, 671
GluI GCNGC 5 cut(s) 286, 294, 675, 841, 915
HapII CCGG 5 cut(s) 375, 651, 948, 1112, 1217
HgaI GACGC 1 cut(s) 1211
HincII GTYRAC 2 cut(s) 589, 1209
HindII GTYRAC 2 cut(s) 589, 1209
HinfI GANTC 7 cut(s) 302, 322, 416, 798, 1026, 1039, 1239
HpaII CCGG 5 cut(s) 375, 651, 948, 1112, 1217
HphI GGTGA 6 cut(s) 139, 322, 875, 1111, 1177, 1412
Hpy166II GTNNAC 5 cut(s) 589, 868, 1054, 1060, 1209
Hpy188I TCNGA 6 cut(s) 104, 301, 1191, 1224, 1358, 1384
Hpy188III TCNNGA 4 cut(s) 449, 651, 923, 1243
Hpy8I GTNNAC 5 cut(s) 589, 868, 1054, 1060, 1209
Hpy99I CGWCG 2 cut(s) 614, 1255
HpyAV CCTTC 2 cut(s) 316, 1366
HpyCH4III ACNGT 4 cut(s) 125, 593, 767, 1424
HpyCH4IV ACGT 4 cut(s) 612, 620, 1056, 1250
HpyF10VI GCNNNNNNNGC 3 cut(s) 183, 911, 1180
HpyF3I CTNAG 4 cut(s) 379, 629, 1190, 1212
HpySE526I ACGT 4 cut(s) 612, 620, 1056, 1250
Kpn2I TCCGGA 1 cut(s) 650
KpnI GGTACC 1 cut(s) 1438
KroI GCCGGC 1 cut(s) 1216
KroNI GCCGGC 1 cut(s) 1218
Ksp22I TGATCA 2 cut(s) 819, 1132
Kzo9I GATC 6 cut(s) 104, 667, 819, 898, 1132, 1167
LmnI GCTCC 4 cut(s) 549, 890, 1024, 1416
Lsp1109I GCAGC 2 cut(s) 297, 305
LweI GCATC 1 cut(s) 632
MaeI CTAG 2 cut(s) 449, 671
MaeII ACGT 4 cut(s) 612, 620, 1056, 1250
MaeIII GTNAC 5 cut(s) 125, 538, 724, 778, 1199
MalI GATC 6 cut(s) 106, 669, 821, 900, 1134, 1169
MboI GATC 6 cut(s) 104, 667, 819, 898, 1132, 1167
MboII GAAGA 6 cut(s) 16, 136, 229, 347, 356, 403
MfeI CAATTG 2 cut(s) 663, 784
MhlI GDGCHC 1 cut(s) 1005
MluI ACGCGT 1 cut(s) 1203
MlyI GAGTC 4 cut(s) 311, 807, 1033, 1035
MmeI TCCRAC 2 cut(s) 286, 1336
Mph1103I ATGCAT 1 cut(s) 993
MroI TCCGGA 1 cut(s) 650
MroNI GCCGGC 1 cut(s) 1216
MroXI GAANNNNTTC 2 cut(s) 326, 864
MseI TTAA 5 cut(s) 59, 81, 960, 971, 1350
MslI CAYNNNNRTG 1 cut(s) 1152
MspA1I CMGCKG 1 cut(s) 977
MspI CCGG 5 cut(s) 375, 651, 948, 1112, 1217
MspR9I CCNGG 2 cut(s) 949, 1112
MunI CAATTG 2 cut(s) 663, 784
MvnI CGCG 1 cut(s) 1205
MwoI GCNNNNNNNGC 3 cut(s) 183, 911, 1180
NaeI GCCGGC 1 cut(s) 1218
NciI CCSGG 2 cut(s) 949, 1112
NcoI CCATGG 1 cut(s) 1412
NdeII GATC 6 cut(s) 104, 667, 819, 898, 1132, 1167
NgoMIV GCCGGC 1 cut(s) 1216
NlaIV GGNNCC 2 cut(s) 185, 1436
NmeAIII GCCGAG 1 cut(s) 869
NmuCI GTSAC 1 cut(s) 1199
NsiI ATGCAT 1 cut(s) 993
NspI RCATGY 4 cut(s) 88, 472, 546, 991
NspV TTCGAA 1 cut(s) 414
OliI CACNNNNGTG 1 cut(s) 1152
PaeI GCATGC 1 cut(s) 991
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PdiI GCCGGC 1 cut(s) 1218
PdmI GAANNNNTTC 2 cut(s) 326, 864
PfeI GAWTC 3 cut(s) 322, 416, 1239
Pfl23II CGTACG 1 cut(s) 310
PfoI TCCNGGA 1 cut(s) 1110
PkrI GCNGC 5 cut(s) 287, 295, 676, 842, 916
PleI GAGTC 4 cut(s) 310, 806, 1033, 1034
PpsI GAGTC 4 cut(s) 310, 806, 1033, 1034
Ppu21I YACGTR 1 cut(s) 1057
PsiI TTATAA 1 cut(s) 909
PspLI CGTACG 1 cut(s) 310
PspN4I GGNNCC 2 cut(s) 185, 1436
RsaI GTAC 6 cut(s) 312, 623, 752, 880, 1061, 1436
RsaNI GTAC 6 cut(s) 311, 622, 751, 879, 1060, 1435
RseI CAYNNNNRTG 1 cut(s) 1152
SaqAI TTAA 5 cut(s) 59, 81, 960, 971, 1350
SatI GCNGC 5 cut(s) 286, 294, 675, 841, 915
Sau3AI GATC 6 cut(s) 104, 667, 819, 898, 1132, 1167
SchI GAGTC 4 cut(s) 311, 807, 1033, 1035
ScrFI CCNGG 2 cut(s) 949, 1112
SduI GDGCHC 1 cut(s) 1005
SfaNI GCATC 1 cut(s) 632
SfuI TTCGAA 1 cut(s) 414
SmiMI CAYNNNNRTG 1 cut(s) 1152
SmlI CTYRAG 1 cut(s) 998
SmoI CTYRAG 1 cut(s) 998
SphI GCATGC 1 cut(s) 991
SsiI CCGC 8 cut(s) 546, 675, 813, 841, 915, 977, 1163, 1183
SspMI CTAG 2 cut(s) 449, 671
StyD4I CCNGG 2 cut(s) 947, 1110
StyI CCWWGG 2 cut(s) 1122, 1412
TaaI ACNGT 4 cut(s) 125, 593, 767, 1424
TaiI ACGT 4 cut(s) 615, 623, 1059, 1253
TaqI TCGA 9 cut(s) 191, 414, 510, 525, 796, 901, 1097, 1170, 1253
TaqII GACCGA 1 cut(s) 1054
TatI WGTACW 1 cut(s) 1059
TauI GCSGC 3 cut(s) 677, 843, 917
TfiI GAWTC 3 cut(s) 322, 416, 1239
Tru1I TTAA 5 cut(s) 59, 81, 960, 971, 1350
Tru9I TTAA 5 cut(s) 59, 81, 960, 971, 1350
TscAI CASTG 1 cut(s) 1041
TseFI GTSAC 1 cut(s) 1199
TseI GCWGC 2 cut(s) 285, 293
Tsp45I GTSAC 1 cut(s) 1199
TspDTI ATGAA 4 cut(s) 17, 156, 340, 468
TspGWI ACGGA 1 cut(s) 903
TspRI CASTG 1 cut(s) 1041
XapI RAATTY 4 cut(s) 19, 26, 347, 1011
XbaI TCTAGA 1 cut(s) 448
XceI RCATGY 4 cut(s) 88, 472, 546, 991
XcmI CCANNNNNNNNNTGG 1 cut(s) 1446
XmnI GAANNNNTTC 2 cut(s) 326, 864
XspI CTAG 2 cut(s) 449, 671
Zsp2I ATGCAT 1 cut(s) 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.