FvH4_2g24420

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015548
Physical Location & Seq
Forward (+)
19942420 .. 19944580
2161 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24420.t1

Sequence Viewer

Length: 1410 bp
ATGGACAGATTGGTGAAAGCCGACGTGAAGGAAGTGGAGCTGAGTTTCAAACGTGATCAGAAGTGCAGCACAACTTTCCGACTCAGCAACCTCATGCACACCATGTCCGTCGCAGTTAACCTAACCACCACAAACCCATCTCTCTTCTCCTTCACGCAACCCTTCTCCATAATCCCTCCGCTCTCTTCCTCCTCCTACACCCTCCTCCTCTCCCACCCCTCCGACCACCCCCCACTCCCCACCACCGCCCCCAATGACGTCATCAACGTCAAGGCCTCGATGCTGCCGACGGGGAAGGCGGGCCTGGATGATCTTCGCCGCCTGTTCGCGAGGCCAGGGCCGCACGTTTTCCGGGACGCCACCATACCGATCTCGTTTGTGGGCCCACAGGTGGTGGAGTTTCTCATTTCTCAGCGGACCAAGATCTCCGGGTTCGATGTCTTTTTCGACAAAGCGGTTTCCGCGTGCTCTGGATCTGAGCTCACGGCGCTGCTTAGTCCTGCTATAGCTTCCGGCAACGCGAGCTTGGTCTCTAGCTTGATCGACGCAGGTGGGGACGTGAATAGAAAGGTTTCGGATTCCGGGTGTTTGATATCTCTGGCTGTTCGGAGCGGGAGTATTGAAGTTGTGAAGATTCTGGTAGCTTCTGGCTGTAAAGTCGATAGCTCGGTTCTGCACGACGCGGCGGCGATGAACCGGGTCGATTTGATAGAGATTCTGTGCAAGAATTTCCCTGAACTTGACGTCAACTGGGTTGACTCCGAAGGCCGGACTCCGATACACGTCGCGGCGGCGAACGGCCACGTGGAGATGTTGAAGTTTTTAGTTTCTGCCGGAGGGGATGGGGAGGTGTGGGACCAGAAGGGGTTGACTCCGCTTCATTGGGCTGCTGAGAAGGGTCACTTGGAGCCGACCAAGTTTTTGTTGGAGTGTTCCGATGTGAAATATGTTGTTACCAAGAATGGAAAGACAGCTTTTGACCTCGCAGCGGAGAATGGTCACTTGAGTTTGCTCGGTCATCTCCGATTCGACGACGTTTTGCTCCGGGCGGCGAGGCTGGACGACGTGCATGGCCTTAAGAGTTGCCTTGCGGAAGGGGCAGAGGTGAATGTGAGGGACCAAAATGGCTGGACCCCTTTGCATAGGGCTGCATTCAAGGGAAGAATTGAGTGTGTCAAGGTGCTGCTTAATCATGGTGCTCAGCTTGATGTGGTGGACAATGCAGGCTACATGCCGCTGCATTGCGCGGTGGAGGCGGGGCATGTTCAAGTAGCTCTTTTGTTGGTGGCGCATGGGGCTCGAGCCAATGTGAAGAGTCTCGAGGGGATTGTGTCGCCAGTTAGCTTGGATCGGTTTAAGAACCACCCTGCTCTTGTTCATCCTGTTTGTCATGAAAAGGAGAGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001508 GO:0002027 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003205 GO:0003230 GO:0003279 GO:0003283 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0006888 GO:0006928 GO:0006936 GO:0006937 GO:0006941 GO:0006942 GO:0007009 GO:0007154 GO:0007165 GO:0007267 GO:0007275 GO:0007507 GO:0008015 GO:0008016 GO:0008092 GO:0008104 GO:0008150 GO:0009893 GO:0009987 GO:0010256 GO:0010468 GO:0010522 GO:0010604 GO:0010628 GO:0010646 GO:0010880 GO:0010881 GO:0010882 GO:0010959 GO:0014704 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0019222 GO:0019722 GO:0019725 GO:0019899 GO:0019900 GO:0019901 GO:0019932 GO:0022898 GO:0023051 GO:0023052 GO:0030001 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030048 GO:0030054 GO:0030154 GO:0030315 GO:0030507 GO:0030674 GO:0031430 GO:0031647 GO:0031672 GO:0031674 GO:0032409 GO:0032411 GO:0032412 GO:0032414 GO:0032501 GO:0032502 GO:0032879 GO:0032970 GO:0033036 GO:0033292 GO:0033365 GO:0034394 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035556 GO:0035637 GO:0036309 GO:0036371 GO:0042383 GO:0042391 GO:0042592 GO:0042692 GO:0043034 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043292 GO:0044057 GO:0044093 GO:0044291 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044459 GO:0044464 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050821 GO:0050896 GO:0051049 GO:0051050 GO:0051117 GO:0051179 GO:0051234 GO:0051239 GO:0051270 GO:0051279 GO:0051282 GO:0051641 GO:0051649 GO:0051716 GO:0051899 GO:0051924 GO:0051928 GO:0055001 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055117 GO:0060047 GO:0060048 GO:0060090 GO:0060255 GO:0060306 GO:0060307 GO:0060341 GO:0061024 GO:0061061 GO:0061337 GO:0065007 GO:0065008 GO:0065009 GO:0070252 GO:0070296 GO:0070727 GO:0070838 GO:0070972 GO:0071840 GO:0071944 GO:0072359 GO:0072503 GO:0072507 GO:0072511 GO:0072657 GO:0072659 GO:0086001 GO:0086002 GO:0086003 GO:0086004 GO:0086005 GO:0086010 GO:0086012 GO:0086014 GO:0086015 GO:0086018 GO:0086019 GO:0086026 GO:0086046 GO:0086065 GO:0086066 GO:0086070 GO:0086091 GO:0090257 GO:0098590 GO:0098771 GO:0098900 GO:0098901 GO:0098907 GO:0098910 GO:0099080 GO:0099081 GO:0099512 GO:0099623 GO:1901016 GO:1901018 GO:1901019 GO:1901021 GO:1901379 GO:1901381 GO:1903115 GO:1903169 GO:1903522 GO:1903779 GO:1904062 GO:1904064 GO:1904427 GO:1990778 GO:2001257 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4

Protein Analysis

470

Amino Acids

50.52

Weight (kDa)

6.46

Isoelectric Point (pI)

30.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 199 - 280 9.5e-15 Ankyrin repeats (3 copies)
Ank_4 PF13637 225 - 276 6.2e-11 Ankyrin repeats (many copies)
Ank_5 PF13857 242 - 277 2.3e-06 Ankyrin repeats (many copies)
Ank PF00023 256 - 281 4.6e-06 Ankyrin repeat
Ank_2 PF12796 286 - 337 9e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 347 - 438 2.1e-19 Ankyrin repeats (3 copies)
Ank_4 PF13637 347 - 396 2.1e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 365 - 413 1.4e-07 Ankyrin repeats (many copies)
Ank PF00023 375 - 407 7.2e-09 Ankyrin repeat
Ank_4 PF13637 383 - 429 2.5e-10 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015548)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24420
malus_domestica MD03G1273500.v1.1 MD11G1296100.v1.1
prunus_persica Prupe.8G249900_v2.0.a1
pyrus_communis pycom03g21790 pycom11g26060
rosa_chinensis RchiOBHm_Chr6g0292251
rosa_laevigata RLG00000012048
rosa_multiflora Rmu_sc0002993.1_g000015
rosa_roxburghii Rroxscaffold_7G00175280
rosa_rugosa Rorug06G0224900
rosa_samantha Rh6AG338200 Rh6BG345500 Rh6CG352400 Rh6DG338400
rosa_wichuraiana Rw6G029430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 539
AatII GACGTC 2 cut(s) 261, 747
Acc36I ACCTGC 1 cut(s) 539
AccBSI CCGCTC 2 cut(s) 181, 612
AccII CGCG 6 cut(s) 329, 464, 521, 683, 788, 1247
AclWI GGATC 2 cut(s) 481, 1356
AcoI YGGCCR 1 cut(s) 799
AcsI RAATTY 1 cut(s) 727
AcvI CACGTG 1 cut(s) 805
AcyI GRCGYC 3 cut(s) 258, 357, 744
AfiI CCNNNNNNNGG 3 cut(s) 391, 768, 988
AflII CTTAAG 1 cut(s) 1076
AflIII ACRYGT 1 cut(s) 781
AgsI TTSAA 5 cut(s) 49, 623, 817, 1156, 1268
AjiI CACGTC 4 cut(s) 25, 559, 784, 1066
AjnI CCWGG 2 cut(s) 303, 334
AjuI GAANNNNNNNTTGG 2 cut(s) 887, 919
Alw21I GWGCWC 3 cut(s) 470, 483, 1201
Alw26I GTCTC 2 cut(s) 535, 1322
AlwI GGATC 2 cut(s) 481, 1356
Ama87I CYCGRG 2 cut(s) 1299, 1319
AoxI GGCC 8 cut(s) 273, 301, 332, 338, 382, 766, 799, 1072
ApaI GGGCCC 1 cut(s) 386
ApeKI GCWGC 8 cut(s) 66, 283, 490, 887, 986, 1148, 1183, 1237
ApoI RAATTY 1 cut(s) 727
ArsI GACNNNNNNTTYG 2 cut(s) 904, 936
Asp700I GAANNNNTTC 1 cut(s) 571
AspLEI GCGC 3 cut(s) 490, 1247, 1291
AspS9I GGNCC 8 cut(s) 301, 338, 382, 383, 417, 856, 1117, 1131
AsuC2I CCSGG 5 cut(s) 353, 430, 583, 698, 1046
AsuHPI GGTGA 2 cut(s) 25, 1117
AvaI CYCGRG 2 cut(s) 1299, 1319
AvaII GGWCC 4 cut(s) 417, 856, 1117, 1131
BaeGI GKGCMC 1 cut(s) 386
BanII GRGCYC 3 cut(s) 386, 483, 1300
BbrPI CACGTG 1 cut(s) 805
Bbv12I GWGCWC 3 cut(s) 470, 483, 1201
BbvI GCAGC 8 cut(s) 78, 270, 477, 874, 998, 1135, 1170, 1224
BccI CCATC 2 cut(s) 145, 836
BceAI ACGGC 2 cut(s) 501, 814
BcgI CGANNNNNNTGC 2 cut(s) 1280, 1314
BciT130I CCWGG 2 cut(s) 305, 336
BclI TGATCA 1 cut(s) 55
BcnI CCSGG 5 cut(s) 353, 430, 583, 698, 1046
BcoDI GTCTC 2 cut(s) 535, 1322
BfaI CTAG 1 cut(s) 534
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 1 cut(s) 491
BfrI CTTAAG 1 cut(s) 1076
BfuAI ACCTGC 1 cut(s) 539
BglII AGATCT 1 cut(s) 423
BlpI GCTNAGC 1 cut(s) 1200
Bme1390I CCNGG 7 cut(s) 305, 336, 353, 430, 583, 698, 1046
Bme18I GGWCC 4 cut(s) 417, 856, 1117, 1131
BmeT110I CYCGRG 2 cut(s) 1299, 1319
BmgBI CACGTC 4 cut(s) 25, 559, 784, 1066
BmgT120I GGNCC 8 cut(s) 301, 338, 382, 383, 417, 856, 1117, 1131
BmiI GGNNCC 5 cut(s) 384, 857, 909, 1118, 1133
BmrFI CCNGG 7 cut(s) 305, 336, 353, 430, 583, 698, 1046
BmrI ACTGGG 1 cut(s) 760
BmsI GCATC 1 cut(s) 270
BmuI ACTGGG 1 cut(s) 760
Bpu1102I GCTNAGC 1 cut(s) 1200
BpuEI CTTGAG 1 cut(s) 1024
BpuMI CCSGG 5 cut(s) 353, 430, 583, 698, 1046
BsaAI YACGTR 1 cut(s) 805
BsaHI GRCGYC 3 cut(s) 258, 357, 744
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 1 cut(s) 335
BsaXI ACNNNNNCTCC 2 cut(s) 601, 631
Bsc4I CCNNNNNNNGG 3 cut(s) 391, 768, 988
Bse1I ACTGG 2 cut(s) 755, 1337
Bse3DI GCAATG 1 cut(s) 1240
BseBI CCWGG 2 cut(s) 305, 336
BseDI CCNNGG 1 cut(s) 335
BseGI GGATG 3 cut(s) 313, 847, 1378
BseLI CCNNNNNNNGG 3 cut(s) 391, 768, 988
BseMI GCAATG 1 cut(s) 1240
BseMII CTCAG 6 cut(s) 32, 97, 425, 468, 882, 1214
BseNI ACTGG 2 cut(s) 755, 1337
BseRI GAGGAG 3 cut(s) 181, 194, 197
BseSI GKGCMC 1 cut(s) 386
BseXI GCAGC 8 cut(s) 78, 270, 477, 874, 998, 1135, 1170, 1224
BsgI GTGCAG 2 cut(s) 85, 659
Bsh1236I CGCG 6 cut(s) 329, 464, 521, 683, 788, 1247
BshFI GGCC 8 cut(s) 275, 303, 334, 340, 384, 768, 801, 1074
BsiHKAI GWGCWC 3 cut(s) 470, 483, 1201
BsiHKCI CYCGRG 2 cut(s) 1299, 1319
BsiSI CCGG 8 cut(s) 352, 429, 513, 582, 697, 769, 834, 1045
BslFI GGGAC 4 cut(s) 368, 569, 869, 1130
BslI CCNNNNNNNGG 3 cut(s) 391, 768, 988
BsmAI GTCTC 2 cut(s) 535, 1322
BsmFI GGGAC 4 cut(s) 368, 569, 869, 1130
BsmI GAATGC 1 cut(s) 1151
BsnI GGCC 8 cut(s) 275, 303, 334, 340, 384, 768, 801, 1074
Bso31I GGTCTC 1 cut(s) 535
BsoBI CYCGRG 2 cut(s) 1299, 1319
Bsp120I GGGCCC 1 cut(s) 382
Bsp1286I GDGCHC 5 cut(s) 386, 470, 483, 1201, 1300
Bsp143I GATC 7 cut(s) 55, 310, 369, 423, 473, 540, 1348
Bsp1720I GCTNAGC 1 cut(s) 1200
Bsp68I TCGCGA 1 cut(s) 329
BspANI GGCC 8 cut(s) 275, 303, 334, 340, 384, 768, 801, 1074
BspCNI CTCAG 6 cut(s) 33, 96, 424, 469, 883, 1213
BspFNI CGCG 6 cut(s) 329, 464, 521, 683, 788, 1247
BspHI TCATGA 1 cut(s) 1390
BspLI GGNNCC 5 cut(s) 384, 857, 909, 1118, 1133
BspMI ACCTGC 1 cut(s) 539
BspPI GGATC 2 cut(s) 481, 1356
BspTI CTTAAG 1 cut(s) 1076
BspTNI GGTCTC 1 cut(s) 535
BsrBI CCGCTC 2 cut(s) 181, 612
BsrDI GCAATG 1 cut(s) 1240
BsrI ACTGG 2 cut(s) 755, 1337
BssECI CCNNGG 1 cut(s) 335
BssMI GATC 7 cut(s) 55, 310, 369, 423, 473, 540, 1348
BssNI GRCGYC 3 cut(s) 258, 357, 744
Bst2UI CCWGG 2 cut(s) 305, 336
Bst6I CTCTTC 3 cut(s) 149, 190, 1307
BstACI GRCGYC 3 cut(s) 258, 357, 744
BstAFI CTTAAG 1 cut(s) 1076
BstBAI YACGTR 1 cut(s) 805
BstC8I GCNNGC 4 cut(s) 301, 466, 523, 1225
BstDEI CTNAG 7 cut(s) 41, 83, 411, 477, 494, 891, 1200
BstF5I GGATG 3 cut(s) 313, 847, 1378
BstFNI CGCG 6 cut(s) 329, 464, 521, 683, 788, 1247
BstH2I RGCGCY 1 cut(s) 491
BstHHI GCGC 3 cut(s) 490, 1247, 1291
BstKTI GATC 7 cut(s) 58, 313, 372, 426, 476, 543, 1351
BstMAI GTCTC 2 cut(s) 535, 1322
BstMBI GATC 7 cut(s) 55, 310, 369, 423, 473, 540, 1348
BstMWI GCNNNNNNNGC 7 cut(s) 340, 461, 487, 522, 1097, 1253, 1295
BstNI CCWGG 2 cut(s) 305, 336
BstNSI RCATGY 2 cut(s) 1234, 1265
BstSCI CCNGG 7 cut(s) 303, 334, 351, 428, 581, 696, 1044
BstSFI CTRYAG 1 cut(s) 504
BstSLI GKGCMC 1 cut(s) 386
BstUI CGCG 6 cut(s) 329, 464, 521, 683, 788, 1247
BstV1I GCAGC 8 cut(s) 78, 270, 477, 874, 998, 1135, 1170, 1224
BstX2I RGATCY 2 cut(s) 423, 473
BstYI RGATCY 2 cut(s) 423, 473
BsuRI GGCC 8 cut(s) 275, 303, 334, 340, 384, 768, 801, 1074
BtgZI GCGATG 1 cut(s) 704
BtrI CACGTC 4 cut(s) 25, 559, 784, 1066
BtsCI GGATG 3 cut(s) 313, 847, 1378
BtuMI TCGCGA 1 cut(s) 329
BveI ACCTGC 1 cut(s) 539
Cac8I GCNNGC 4 cut(s) 301, 466, 523, 1225
CciI TCATGA 1 cut(s) 1390
CfoI GCGC 3 cut(s) 490, 1247, 1291
Cfr13I GGNCC 8 cut(s) 301, 338, 382, 383, 417, 856, 1117, 1131
CseI GACGC 3 cut(s) 365, 554, 689
CviAII CATG 8 cut(s) 94, 103, 1070, 1193, 1231, 1262, 1292, 1391
DdeI CTNAG 7 cut(s) 41, 83, 411, 477, 494, 891, 1200
DpnI GATC 7 cut(s) 57, 312, 371, 425, 475, 542, 1350
DpnII GATC 7 cut(s) 55, 310, 369, 423, 473, 540, 1348
EaeI YGGCCR 1 cut(s) 799
Eam1104I CTCTTC 3 cut(s) 149, 190, 1307
EarI CTCTTC 3 cut(s) 149, 190, 1307
Ecl136II GAGCTC 1 cut(s) 481
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 3 cut(s) 386, 483, 1300
Eco31I GGTCTC 1 cut(s) 535
Eco32I GATATC 1 cut(s) 594
Eco47I GGWCC 4 cut(s) 417, 856, 1117, 1131
Eco53kI GAGCTC 1 cut(s) 481
Eco72I CACGTG 1 cut(s) 805
Eco88I CYCGRG 2 cut(s) 1299, 1319
EcoICRI GAGCTC 1 cut(s) 481
EcoRII CCWGG 2 cut(s) 303, 334
EcoRV GATATC 1 cut(s) 594
EcoT38I GRGCYC 3 cut(s) 386, 483, 1300
FaeI CATG 8 cut(s) 97, 106, 1073, 1196, 1234, 1265, 1295, 1394
FalI AAGNNNNNCTT 4 cut(s) 887, 919, 1260, 1292
FaqI GGGAC 4 cut(s) 368, 569, 869, 1130
FatI CATG 8 cut(s) 93, 102, 1069, 1192, 1230, 1261, 1291, 1390
FauI CCCGC 3 cut(s) 292, 605, 1249
FbaI TGATCA 1 cut(s) 55
FokI GGATG 3 cut(s) 320, 854, 1365
FriOI GRGCYC 3 cut(s) 386, 483, 1300
FspBI CTAG 1 cut(s) 534
GlaI GCGC 3 cut(s) 489, 1246, 1290
HaeII RGCGCY 1 cut(s) 491
HaeIII GGCC 8 cut(s) 275, 303, 334, 340, 384, 768, 801, 1074
HapII CCGG 8 cut(s) 352, 429, 513, 582, 697, 769, 834, 1045
HgaI GACGC 3 cut(s) 365, 554, 689
HhaI GCGC 3 cut(s) 490, 1247, 1291
Hin1I GRCGYC 3 cut(s) 258, 357, 744
Hin1II CATG 8 cut(s) 97, 106, 1073, 1196, 1234, 1265, 1295, 1394
Hin6I GCGC 3 cut(s) 488, 1245, 1289
HinP1I GCGC 3 cut(s) 488, 1245, 1289
HincII GTYRAC 4 cut(s) 118, 748, 757, 870
HindII GTYRAC 4 cut(s) 118, 748, 757, 870
HinfI GANTC 9 cut(s) 81, 578, 634, 715, 758, 772, 871, 1026, 1315
HpaI GTTAAC 1 cut(s) 118
HpaII CCGG 8 cut(s) 352, 429, 513, 582, 697, 769, 834, 1045
HphI GGTGA 2 cut(s) 25, 1117
Hpy166II GTNNAC 5 cut(s) 118, 748, 757, 870, 1216
Hpy188III TCNNGA 4 cut(s) 328, 471, 1319, 1391
Hpy8I GTNNAC 5 cut(s) 118, 748, 757, 870, 1216
Hpy99I CGWCG 9 cut(s) 26, 113, 292, 548, 683, 788, 1034, 1037, 1067
HpyAV CCTTC 8 cut(s) 22, 160, 172, 289, 758, 856, 889, 1088
HpyCH4V TGCA 9 cut(s) 66, 97, 676, 723, 1069, 1141, 1151, 1223, 1240
HpyF10VI GCNNNNNNNGC 7 cut(s) 340, 461, 487, 522, 1097, 1253, 1295
HpyF3I CTNAG 7 cut(s) 41, 83, 411, 477, 494, 891, 1200
Hsp92I GRCGYC 3 cut(s) 258, 357, 744
Hsp92II CATG 8 cut(s) 97, 106, 1073, 1196, 1234, 1265, 1295, 1394
HspAI GCGC 3 cut(s) 488, 1245, 1289
Ksp22I TGATCA 1 cut(s) 55
KspAI GTTAAC 1 cut(s) 118
Kzo9I GATC 7 cut(s) 55, 310, 369, 423, 473, 540, 1348
LmnI GCTCC 4 cut(s) 37, 609, 907, 1047
Lsp1109I GCAGC 8 cut(s) 78, 270, 477, 874, 998, 1135, 1170, 1224
LweI GCATC 1 cut(s) 270
MaeI CTAG 1 cut(s) 534
MaeIII GTNAC 3 cut(s) 899, 952, 998
MalI GATC 7 cut(s) 57, 312, 371, 425, 475, 542, 1350
MbiI CCGCTC 2 cut(s) 181, 612
MboI GATC 7 cut(s) 55, 310, 369, 423, 473, 540, 1348
MboII GAAGA 6 cut(s) 136, 177, 305, 643, 1173, 1324
MflI RGATCY 2 cut(s) 423, 473
MhlI GDGCHC 5 cut(s) 386, 470, 483, 1201, 1300
MluCI AATT 2 cut(s) 727, 1164
MlyI GAGTC 5 cut(s) 75, 752, 766, 865, 1324
MmeI TCCRAC 3 cut(s) 103, 246, 906
MroXI GAANNNNTTC 1 cut(s) 571
MseI TTAA 4 cut(s) 117, 1077, 1188, 1356
MspA1I CMGCKG 3 cut(s) 415, 989, 1237
MspCI CTTAAG 1 cut(s) 1076
MspI CCGG 8 cut(s) 352, 429, 513, 582, 697, 769, 834, 1045
MspR9I CCNGG 7 cut(s) 305, 336, 353, 430, 583, 698, 1046
Mva1269I GAATGC 1 cut(s) 1151
MvaI CCWGG 2 cut(s) 305, 336
MvnI CGCG 6 cut(s) 329, 464, 521, 683, 788, 1247
MwoI GCNNNNNNNGC 7 cut(s) 340, 461, 487, 522, 1097, 1253, 1295
NciI CCSGG 5 cut(s) 353, 430, 583, 698, 1046
NdeII GATC 7 cut(s) 55, 310, 369, 423, 473, 540, 1348
NlaIII CATG 8 cut(s) 97, 106, 1073, 1196, 1234, 1265, 1295, 1394
NlaIV GGNNCC 5 cut(s) 384, 857, 909, 1118, 1133
NmuCI GTSAC 2 cut(s) 899, 998
NruI TCGCGA 1 cut(s) 329
NspI RCATGY 2 cut(s) 1234, 1265
PaeR7I CTCGAG 2 cut(s) 1299, 1319
PagI TCATGA 1 cut(s) 1390
PaqCI CACCTGC 1 cut(s) 539
PceI AGGCCT 1 cut(s) 275
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 284
PctI GAATGC 1 cut(s) 1151
PdmI GAANNNNTTC 1 cut(s) 571
PfeI GAWTC 4 cut(s) 578, 634, 715, 1026
PfoI TCCNGGA 1 cut(s) 351
PleI GAGTC 5 cut(s) 75, 752, 766, 865, 1323
PmaCI CACGTG 1 cut(s) 805
PmlI CACGTG 1 cut(s) 805
PpsI GAGTC 5 cut(s) 75, 752, 766, 865, 1323
Ppu21I YACGTR 1 cut(s) 805
Psp124BI GAGCTC 1 cut(s) 483
Psp6I CCWGG 2 cut(s) 303, 334
PspCI CACGTG 1 cut(s) 805
PspGI CCWGG 2 cut(s) 303, 334
PspN4I GGNNCC 5 cut(s) 384, 857, 909, 1118, 1133
PspOMI GGGCCC 1 cut(s) 382
PspPI GGNCC 8 cut(s) 301, 338, 382, 383, 417, 856, 1117, 1131
PspXI VCTCGAGB 1 cut(s) 1299
PsuI RGATCY 2 cut(s) 423, 473
RruI TCGCGA 1 cut(s) 329
SacI GAGCTC 1 cut(s) 483
SaqAI TTAA 4 cut(s) 117, 1077, 1188, 1356
Sau3AI GATC 7 cut(s) 55, 310, 369, 423, 473, 540, 1348
Sau96I GGNCC 8 cut(s) 301, 338, 382, 383, 417, 856, 1117, 1131
SchI GAGTC 5 cut(s) 75, 752, 766, 865, 1324
ScrFI CCNGG 7 cut(s) 305, 336, 353, 430, 583, 698, 1046
SduI GDGCHC 5 cut(s) 386, 470, 483, 1201, 1300
SfaNI GCATC 1 cut(s) 270
SfcI CTRYAG 1 cut(s) 504
Sfr274I CTCGAG 2 cut(s) 1299, 1319
SinI GGWCC 4 cut(s) 417, 856, 1117, 1131
SlaI CTCGAG 2 cut(s) 1299, 1319
SmlI CTYRAG 4 cut(s) 1003, 1076, 1299, 1319
SmoI CTYRAG 4 cut(s) 1003, 1076, 1299, 1319
Sse9I AATT 2 cut(s) 727, 1164
SseBI AGGCCT 1 cut(s) 275
SspMI CTAG 1 cut(s) 534
SstI GAGCTC 1 cut(s) 483
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 7 cut(s) 303, 334, 351, 428, 581, 696, 1044
TaqI TCGA 9 cut(s) 278, 435, 447, 543, 660, 702, 1029, 1300, 1320
TaqII GACCGA 1 cut(s) 1004
TasI AATT 2 cut(s) 727, 1164
TauI GCSGC 8 cut(s) 321, 343, 686, 689, 791, 794, 1052, 1237
TfiI GAWTC 4 cut(s) 578, 634, 715, 1026
Tru1I TTAA 4 cut(s) 117, 1077, 1188, 1356
Tru9I TTAA 4 cut(s) 117, 1077, 1188, 1356
TseFI GTSAC 2 cut(s) 899, 998
TseI GCWGC 8 cut(s) 66, 283, 490, 887, 986, 1148, 1183, 1237
Tsp45I GTSAC 2 cut(s) 899, 998
TspDTI ATGAA 4 cut(s) 707, 869, 1367, 1407
TspGWI ACGGA 1 cut(s) 97
Vha464I CTTAAG 1 cut(s) 1076
VpaK11BI GGWCC 4 cut(s) 417, 856, 1117, 1131
XapI RAATTY 1 cut(s) 727
XceI RCATGY 2 cut(s) 1234, 1265
XcmI CCANNNNNNNNNTGG 1 cut(s) 922
XhoI CTCGAG 2 cut(s) 1299, 1319
XmnI GAANNNNTTC 1 cut(s) 571
XspI CTAG 1 cut(s) 534
ZraI GACGTC 2 cut(s) 259, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.