RchiOBHm_Chr6g0292251

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015548
Physical Location & Seq
Forward (+)
55205513 .. 55207369
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26225

Sequence Viewer

Length: 1410 bp
ATGGACAGATTGGTGAAGGCGGACGTGAAGGAAGTGGAGCTCAGCTTCAAAAGGGACCAGAAGTGCGGCACAACTTTCAGGCTCAGCAACCTCATGCACACCATGTCCGTCGCAGTGAACCTAACCACCACAAACCCATCTCTCTTCTCTTTCTCACAGCCCTTCTCTATAATCCCTCCTCTCTCTTCCTCCTCCTACACCCTCCTCCTCTCTCAGCCCTCCGATCATCCCCCTCTCCCCACCACCGTCCCTAATGACGTCATCAACGTCAAGGCCTCGATGCTGCCGACAGGGAAGGCCGGCCTGGAGGGTCTCCGCCGTCTGTTCGCTAGACCAGGGCCGCACGTTTTCCGGGATGCCACCATACCCATCTCGTTTGTGGGCCCACACGTGGTGGAGTTTCTCATTTCTCAGCGGACCCAGATCTCTGGGTTCGATTTCTTCTTCAACAAAGCGGTTTCCGGGTGCTCTGGATCGGAGCTCACGGAGCTGCTCAGACCCGCCATAGCTTCCGGCAACGCGAACTTGGTCTCGAGCTTGATCGACGCCGGGGGAGATGTGAATCCGAAAGTTTCGGATTCCGGGTGTTGGTTGTCTCTGGCTGTTCGGAGCGGAAGTATTGAAGTTGTGAAGATTCTTATAGCGTGCGGCTGTAAAGTCGATAACTCGGTTTTGCACGACGCGGTGGCGATGAACCGGGTCGATTTGGTGGACATTTTGTGCAAGAATTTCCCGGAAATTGACGTCAACTGGGCTGACTCCGATGGCCGGACTCCGATTCACATCGCGGCTGCGAACGGCCACGTGGAGATGTTGAAGTTCTGTATTTCGGCCGGCGGTAATGCGGACGTTTGGGACCGGAAGGGGTGGACGCCGCTCCATTGCGCGGCGGAGAAGGGTCACTTGGAGCCCATCAAGTCTTTGTTGGAATGCTCCAATGTGAAATATGTAGTTAACAAGAATGGAAAGACAGCTTTTGACCTCGCAGCTGAAAATGGTCACTTAAATTTGCTCTGTTTTCTCCGATTCGACGACTTTTTGCTCCGGGCGGCGAGGCTGGACGACGCTCACGGCCTAAAAAGTTGCCTTGCGGAAGGGGCAGAGGTGAATGGGAGGGACCAAAATGGTTGGACCCCTTTGCATAGGGCTGCATTCAAGGGCAGAATCGAGTGTGTCAAGGTGCTGCTCAATCACAGTGCTCAGCTGGATGTCGTCGATGATGCAGGCTACACGCCGCTGCATTGCGCGGTGGAGGCCGGGCATGTACAAGTAGCTCTTTTGTTGGTTGCGCATGGGGCTCGAGCCAATGTGAAGAGCCTCGAGGGCATTGTGTCGCCGGTTAGCTTGGATCGTTTTAAGAACCACCCTGCTCTTGTCCATCCTGTATGTCATGAACAGGAGAGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001508 GO:0002027 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003205 GO:0003230 GO:0003279 GO:0003283 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0006888 GO:0006928 GO:0006936 GO:0006937 GO:0006941 GO:0006942 GO:0007009 GO:0007154 GO:0007165 GO:0007267 GO:0007275 GO:0007507 GO:0008015 GO:0008016 GO:0008092 GO:0008104 GO:0008150 GO:0009893 GO:0009987 GO:0010256 GO:0010468 GO:0010522 GO:0010604 GO:0010628 GO:0010646 GO:0010880 GO:0010881 GO:0010882 GO:0010959 GO:0014704 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0019222 GO:0019722 GO:0019725 GO:0019899 GO:0019900 GO:0019901 GO:0019932 GO:0022898 GO:0023051 GO:0023052 GO:0030001 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030048 GO:0030054 GO:0030154 GO:0030315 GO:0030507 GO:0030674 GO:0031430 GO:0031647 GO:0031672 GO:0031674 GO:0032409 GO:0032411 GO:0032412 GO:0032414 GO:0032501 GO:0032502 GO:0032879 GO:0032970 GO:0033036 GO:0033292 GO:0033365 GO:0034394 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035556 GO:0035637 GO:0036309 GO:0036371 GO:0042383 GO:0042391 GO:0042592 GO:0042692 GO:0043034 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043292 GO:0044057 GO:0044093 GO:0044291 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044459 GO:0044464 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050821 GO:0050896 GO:0051049 GO:0051050 GO:0051117 GO:0051179 GO:0051234 GO:0051239 GO:0051270 GO:0051279 GO:0051282 GO:0051641 GO:0051649 GO:0051716 GO:0051899 GO:0051924 GO:0051928 GO:0055001 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055117 GO:0060047 GO:0060048 GO:0060090 GO:0060255 GO:0060306 GO:0060307 GO:0060341 GO:0061024 GO:0061061 GO:0061337 GO:0065007 GO:0065008 GO:0065009 GO:0070252 GO:0070296 GO:0070727 GO:0070838 GO:0070972 GO:0071840 GO:0071944 GO:0072359 GO:0072503 GO:0072507 GO:0072511 GO:0072657 GO:0072659 GO:0086001 GO:0086002 GO:0086003 GO:0086004 GO:0086005 GO:0086010 GO:0086012 GO:0086014 GO:0086015 GO:0086018 GO:0086019 GO:0086026 GO:0086046 GO:0086065 GO:0086066 GO:0086070 GO:0086091 GO:0090257 GO:0098590 GO:0098771 GO:0098900 GO:0098901 GO:0098907 GO:0098910 GO:0099080 GO:0099081 GO:0099512 GO:0099623 GO:1901016 GO:1901018 GO:1901019 GO:1901021 GO:1901379 GO:1901381 GO:1903115 GO:1903169 GO:1903522 GO:1903779 GO:1904062 GO:1904064 GO:1904427 GO:1990778 GO:2001257 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4

Protein Analysis

469

Amino Acids

50.77

Weight (kDa)

6.55

Isoelectric Point (pI)

29.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 168 - 249 1.2e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 198 - 278 6.2e-14 Ankyrin repeats (3 copies)
Ank_2 PF12796 225 - 311 2.9e-17 Ankyrin repeats (3 copies)
Ank_4 PF13637 225 - 274 1.6e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 242 - 276 8.1e-06 Ankyrin repeats (many copies)
Ank PF00023 255 - 284 1.7e-06 Ankyrin repeat
Ank_4 PF13637 267 - 303 6.4e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 279 - 341 8.7e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 347 - 438 1.4e-19 Ankyrin repeats (3 copies)
Ank_4 PF13637 357 - 396 4.4e-08 Ankyrin repeats (many copies)
Ank PF00023 375 - 407 9.1e-08 Ankyrin repeat
Ank_4 PF13637 380 - 429 1e-12 Ankyrin repeats (many copies)
Ank PF00023 409 - 438 3.6e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015548)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24420
malus_domestica MD03G1273500.v1.1 MD11G1296100.v1.1
prunus_persica Prupe.8G249900_v2.0.a1
pyrus_communis pycom03g21790 pycom11g26060
rosa_chinensis RchiOBHm_Chr6g0292251
rosa_laevigata RLG00000012048
rosa_multiflora Rmu_sc0002993.1_g000015
rosa_roxburghii Rroxscaffold_7G00175280
rosa_rugosa Rorug06G0224900
rosa_samantha Rh6AG338200 Rh6BG345500 Rh6CG352400 Rh6DG338400
rosa_wichuraiana Rw6G029430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 261, 747
AbsI CCTCGAGG 1 cut(s) 1319
Acc16I TGCGCA 1 cut(s) 1290
AccBSI CCGCTC 2 cut(s) 612, 877
AccII CGCG 5 cut(s) 521, 683, 788, 887, 1247
AclWI GGATC 2 cut(s) 481, 1356
AcoI YGGCCR 3 cut(s) 766, 799, 831
AcsI RAATTY 2 cut(s) 727, 1006
AcvI CACGTG 2 cut(s) 391, 805
AcyI GRCGYC 4 cut(s) 258, 546, 744, 872
AdeI CACNNNGTG 1 cut(s) 394
AfaI GTAC 1 cut(s) 1266
AfiI CCNNNNNNNGG 4 cut(s) 391, 588, 768, 886
AflIII ACRYGT 1 cut(s) 388
AgsI TTSAA 5 cut(s) 49, 448, 623, 817, 1156
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 2 cut(s) 303, 334
AjuI GAANNNNNNNTTGG 2 cut(s) 887, 919
Alw21I GWGCWC 4 cut(s) 42, 470, 483, 1201
Alw26I GTCTC 3 cut(s) 317, 535, 600
AlwI GGATC 2 cut(s) 481, 1356
Ama87I CYCGRG 3 cut(s) 532, 1299, 1319
ApaI GGGCCC 1 cut(s) 386
ApeKI GCWGC 7 cut(s) 283, 490, 791, 986, 1148, 1183, 1237
ApoI RAATTY 2 cut(s) 727, 1006
AspLEI GCGC 3 cut(s) 887, 1247, 1291
AspS9I GGNCC 8 cut(s) 55, 338, 382, 383, 417, 856, 1117, 1131
AsuC2I CCSGG 8 cut(s) 353, 463, 550, 583, 698, 734, 1046, 1258
AsuHPI GGTGA 2 cut(s) 25, 1117
AvaI CYCGRG 3 cut(s) 532, 1299, 1319
AvaII GGWCC 5 cut(s) 55, 417, 856, 1117, 1131
BaeGI GKGCMC 1 cut(s) 386
BanII GRGCYC 5 cut(s) 42, 386, 483, 912, 1300
BbrPI CACGTG 2 cut(s) 391, 805
Bbv12I GWGCWC 4 cut(s) 42, 470, 483, 1201
BbvI GCAGC 7 cut(s) 270, 477, 778, 998, 1135, 1170, 1224
BccI CCATC 5 cut(s) 145, 377, 758, 920, 1386
BceAI ACGGC 3 cut(s) 303, 814, 1087
BcgI CGANNNNNNTGC 2 cut(s) 1280, 1314
BciT130I CCWGG 2 cut(s) 305, 336
BcnI CCSGG 8 cut(s) 353, 463, 550, 583, 698, 734, 1046, 1258
BcoDI GTCTC 3 cut(s) 317, 535, 600
BfaI CTAG 1 cut(s) 330
BglI GCCNNNNNGGC 1 cut(s) 1323
BglII AGATCT 1 cut(s) 423
BlpI GCTNAGC 3 cut(s) 41, 83, 1200
Bme18I GGWCC 5 cut(s) 55, 417, 856, 1117, 1131
BmeT110I CYCGRG 3 cut(s) 532, 1299, 1319
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 8 cut(s) 55, 338, 382, 383, 417, 856, 1117, 1131
BmiI GGNNCC 7 cut(s) 56, 384, 419, 857, 909, 1118, 1133
BmrI ACTGGG 1 cut(s) 760
BmsI GCATC 3 cut(s) 270, 346, 1210
BmuI ACTGGG 1 cut(s) 760
BpmI CTGGAG 1 cut(s) 326
Bpu1102I GCTNAGC 3 cut(s) 41, 83, 1200
BpuMI CCSGG 8 cut(s) 353, 463, 550, 583, 698, 734, 1046, 1258
BsaAI YACGTR 2 cut(s) 391, 805
BsaBI GATNNNNATC 2 cut(s) 561, 782
BsaHI GRCGYC 4 cut(s) 258, 546, 744, 872
BsaI GGTCTC 2 cut(s) 317, 535
BsaJI CCNNGG 2 cut(s) 335, 549
BsaWI WCCGGW 1 cut(s) 858
BsaXI ACNNNNNCTCC 2 cut(s) 601, 631
Bsc4I CCNNNNNNNGG 4 cut(s) 391, 588, 768, 886
Bse118I RCCGGY 3 cut(s) 299, 833, 1336
Bse1I ACTGG 1 cut(s) 755
Bse3DI GCAATG 2 cut(s) 880, 1240
Bse8I GATNNNNATC 2 cut(s) 561, 782
BseBI CCWGG 2 cut(s) 305, 336
BseDI CCNNGG 2 cut(s) 335, 549
BseGI GGATG 4 cut(s) 226, 361, 1213, 1378
BseJI GATNNNNATC 2 cut(s) 561, 782
BseLI CCNNNNNNNGG 4 cut(s) 391, 588, 768, 886
BseMI GCAATG 2 cut(s) 880, 1240
BseMII CTCAG 6 cut(s) 55, 97, 227, 425, 508, 1214
BseNI ACTGG 1 cut(s) 755
BseRI GAGGAG 4 cut(s) 168, 181, 194, 197
BseSI GKGCMC 1 cut(s) 386
BseX3I CGGCCG 1 cut(s) 831
BseXI GCAGC 7 cut(s) 270, 477, 778, 998, 1135, 1170, 1224
Bsh1236I CGCG 5 cut(s) 521, 683, 788, 887, 1247
Bsh1285I CGRYCG 1 cut(s) 834
BsiEI CGRYCG 1 cut(s) 834
BsiHKAI GWGCWC 4 cut(s) 42, 470, 483, 1201
BsiHKCI CYCGRG 3 cut(s) 532, 1299, 1319
BslFI GGGAC 4 cut(s) 68, 233, 869, 1130
BslI CCNNNNNNNGG 4 cut(s) 391, 588, 768, 886
BsmAI GTCTC 3 cut(s) 317, 535, 600
BsmFI GGGAC 4 cut(s) 68, 233, 869, 1130
BsmI GAATGC 2 cut(s) 935, 1151
Bso31I GGTCTC 2 cut(s) 317, 535
BsoBI CYCGRG 3 cut(s) 532, 1299, 1319
Bsp120I GGGCCC 1 cut(s) 382
Bsp1286I GDGCHC 7 cut(s) 42, 386, 470, 483, 912, 1201, 1300
Bsp1407I TGTACA 1 cut(s) 1264
Bsp143I GATC 5 cut(s) 223, 423, 473, 540, 1348
Bsp1720I GCTNAGC 3 cut(s) 41, 83, 1200
BspCNI CTCAG 6 cut(s) 54, 96, 226, 424, 507, 1213
BspFNI CGCG 5 cut(s) 521, 683, 788, 887, 1247
BspHI TCATGA 1 cut(s) 1390
BspLI GGNNCC 7 cut(s) 56, 384, 419, 857, 909, 1118, 1133
BspPI GGATC 2 cut(s) 481, 1356
BspQI GCTCTTC 1 cut(s) 1307
BspTNI GGTCTC 2 cut(s) 317, 535
BsrBI CCGCTC 2 cut(s) 612, 877
BsrDI GCAATG 2 cut(s) 880, 1240
BsrFI RCCGGY 3 cut(s) 299, 833, 1336
BsrGI TGTACA 1 cut(s) 1264
BsrI ACTGG 1 cut(s) 755
BssAI RCCGGY 3 cut(s) 299, 833, 1336
BssECI CCNNGG 2 cut(s) 335, 549
BssMI GATC 5 cut(s) 223, 423, 473, 540, 1348
BssNI GRCGYC 4 cut(s) 258, 546, 744, 872
Bst2UI CCWGG 2 cut(s) 305, 336
Bst4CI ACNGT 2 cut(s) 247, 1196
Bst6I CTCTTC 3 cut(s) 149, 190, 1307
BstACI GRCGYC 4 cut(s) 258, 546, 744, 872
BstAUI TGTACA 1 cut(s) 1264
BstBAI YACGTR 2 cut(s) 391, 805
BstC8I GCNNGC 4 cut(s) 301, 646, 835, 1225
BstDEI CTNAG 6 cut(s) 41, 83, 213, 411, 494, 1200
BstF5I GGATG 4 cut(s) 226, 361, 1213, 1378
BstFNI CGCG 5 cut(s) 521, 683, 788, 887, 1247
BstHHI GCGC 3 cut(s) 887, 1247, 1291
BstKTI GATC 5 cut(s) 226, 426, 476, 543, 1351
BstMAI GTCTC 3 cut(s) 317, 535, 600
BstMBI GATC 5 cut(s) 223, 423, 473, 540, 1348
BstMCI CGRYCG 1 cut(s) 834
BstMWI GCNNNNNNNGC 5 cut(s) 487, 1097, 1253, 1295, 1323
BstNI CCWGG 2 cut(s) 305, 336
BstNSI RCATGY 1 cut(s) 1265
BstSLI GKGCMC 1 cut(s) 386
BstUI CGCG 5 cut(s) 521, 683, 788, 887, 1247
BstV1I GCAGC 7 cut(s) 270, 477, 778, 998, 1135, 1170, 1224
BstX2I RGATCY 1 cut(s) 423
BstXI CCANNNNNNTGG 1 cut(s) 428
BstYI RGATCY 1 cut(s) 423
BstZI CGGCCG 1 cut(s) 831
BtgZI GCGATG 2 cut(s) 704, 769
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 4 cut(s) 226, 361, 1213, 1378
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 2 cut(s) 120, 1201
Cac8I GCNNGC 4 cut(s) 301, 646, 835, 1225
CciI TCATGA 1 cut(s) 1390
CfoI GCGC 3 cut(s) 887, 1247, 1291
Cfr10I RCCGGY 3 cut(s) 299, 833, 1336
Cfr13I GGNCC 8 cut(s) 55, 338, 382, 383, 417, 856, 1117, 1131
CseI GACGC 4 cut(s) 554, 689, 880, 1073
Csp6I GTAC 1 cut(s) 1265
CviAII CATG 5 cut(s) 94, 103, 1262, 1292, 1391
CviQI GTAC 1 cut(s) 1265
DdeI CTNAG 6 cut(s) 41, 83, 213, 411, 494, 1200
DpnI GATC 5 cut(s) 225, 425, 475, 542, 1350
DpnII GATC 5 cut(s) 223, 423, 473, 540, 1348
DraIII CACNNNGTG 1 cut(s) 394
EaeI YGGCCR 3 cut(s) 766, 799, 831
EagI CGGCCG 1 cut(s) 831
Eam1104I CTCTTC 3 cut(s) 149, 190, 1307
EarI CTCTTC 3 cut(s) 149, 190, 1307
EciI GGCGGA 3 cut(s) 35, 305, 905
Ecl136II GAGCTC 2 cut(s) 40, 481
EclXI CGGCCG 1 cut(s) 831
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 5 cut(s) 42, 386, 483, 912, 1300
Eco31I GGTCTC 2 cut(s) 317, 535
Eco47I GGWCC 5 cut(s) 55, 417, 856, 1117, 1131
Eco52I CGGCCG 1 cut(s) 831
Eco53kI GAGCTC 2 cut(s) 40, 481
Eco72I CACGTG 2 cut(s) 391, 805
Eco88I CYCGRG 3 cut(s) 532, 1299, 1319
EcoICRI GAGCTC 2 cut(s) 40, 481
EcoRII CCWGG 2 cut(s) 303, 334
EcoT38I GRGCYC 5 cut(s) 42, 386, 483, 912, 1300
FaeI CATG 5 cut(s) 97, 106, 1265, 1295, 1394
FalI AAGNNNNNCTT 4 cut(s) 887, 919, 1260, 1292
FaqI GGGAC 4 cut(s) 68, 233, 869, 1130
FatI CATG 5 cut(s) 93, 102, 1261, 1291, 1390
FauI CCCGC 1 cut(s) 508
FokI GGATG 4 cut(s) 213, 368, 1220, 1365
FriOI GRGCYC 5 cut(s) 42, 386, 483, 912, 1300
FseI GGCCGGCC 1 cut(s) 303
FspBI CTAG 1 cut(s) 330
FspI TGCGCA 1 cut(s) 1290
GlaI GCGC 3 cut(s) 886, 1246, 1290
GsuI CTGGAG 1 cut(s) 326
HgaI GACGC 4 cut(s) 554, 689, 880, 1073
HhaI GCGC 3 cut(s) 887, 1247, 1291
Hin1I GRCGYC 4 cut(s) 258, 546, 744, 872
Hin1II CATG 5 cut(s) 97, 106, 1265, 1295, 1394
Hin6I GCGC 3 cut(s) 885, 1245, 1289
HinP1I GCGC 3 cut(s) 885, 1245, 1289
HincII GTYRAC 2 cut(s) 748, 955
HindII GTYRAC 2 cut(s) 748, 955
HinfI GANTC 8 cut(s) 562, 578, 634, 758, 772, 778, 1026, 1164
HpaI GTTAAC 1 cut(s) 955
HphI GGTGA 2 cut(s) 25, 1117
Hpy166II GTNNAC 5 cut(s) 118, 712, 748, 870, 955
Hpy188I TCNGA 9 cut(s) 223, 478, 497, 567, 577, 609, 763, 777, 1025
Hpy188III TCNNGA 3 cut(s) 471, 532, 1391
Hpy8I GTNNAC 5 cut(s) 118, 712, 748, 870, 955
Hpy99I CGWCG 6 cut(s) 113, 548, 683, 1034, 1067, 1217
HpyAV CCTTC 7 cut(s) 10, 22, 172, 289, 856, 889, 1088
HpyCH4III ACNGT 2 cut(s) 247, 1196
HpyCH4IV ACGT 8 cut(s) 24, 258, 267, 345, 390, 744, 804, 849
HpyCH4V TGCA 7 cut(s) 97, 676, 723, 1141, 1151, 1223, 1240
HpyF10VI GCNNNNNNNGC 5 cut(s) 487, 1097, 1253, 1295, 1323
HpyF3I CTNAG 6 cut(s) 41, 83, 213, 411, 494, 1200
HpySE526I ACGT 8 cut(s) 24, 258, 267, 345, 390, 744, 804, 849
Hsp92I GRCGYC 4 cut(s) 258, 546, 744, 872
Hsp92II CATG 5 cut(s) 97, 106, 1265, 1295, 1394
HspAI GCGC 3 cut(s) 885, 1245, 1289
KroI GCCGGC 2 cut(s) 299, 833
KroNI GCCGGC 2 cut(s) 301, 835
KspAI GTTAAC 1 cut(s) 955
Kzo9I GATC 5 cut(s) 223, 423, 473, 540, 1348
LguI GCTCTTC 1 cut(s) 1307
LmnI GCTCC 8 cut(s) 37, 478, 487, 609, 882, 907, 938, 1047
Lsp1109I GCAGC 7 cut(s) 270, 477, 778, 998, 1135, 1170, 1224
LweI GCATC 3 cut(s) 270, 346, 1210
MaeI CTAG 1 cut(s) 330
MaeII ACGT 8 cut(s) 24, 258, 267, 345, 390, 744, 804, 849
MaeIII GTNAC 2 cut(s) 899, 998
MalI GATC 5 cut(s) 225, 425, 475, 542, 1350
MbiI CCGCTC 2 cut(s) 612, 877
MboI GATC 5 cut(s) 223, 423, 473, 540, 1348
MboII GAAGA 6 cut(s) 136, 177, 433, 436, 643, 1324
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 7 cut(s) 42, 386, 470, 483, 912, 1201, 1300
MluCI AATT 3 cut(s) 727, 738, 1006
MlyI GAGTC 2 cut(s) 752, 766
MmeI TCCRAC 2 cut(s) 906, 1109
MroNI GCCGGC 2 cut(s) 299, 833
MseI TTAA 3 cut(s) 954, 1004, 1356
MspA1I CMGCKG 4 cut(s) 415, 989, 1204, 1237
Mva1269I GAATGC 2 cut(s) 935, 1151
MvaI CCWGG 2 cut(s) 305, 336
MvnI CGCG 5 cut(s) 521, 683, 788, 887, 1247
MwoI GCNNNNNNNGC 5 cut(s) 487, 1097, 1253, 1295, 1323
NaeI GCCGGC 2 cut(s) 301, 835
NciI CCSGG 8 cut(s) 353, 463, 550, 583, 698, 734, 1046, 1258
NdeII GATC 5 cut(s) 223, 423, 473, 540, 1348
NgoMIV GCCGGC 2 cut(s) 299, 833
NlaIII CATG 5 cut(s) 97, 106, 1265, 1295, 1394
NlaIV GGNNCC 7 cut(s) 56, 384, 419, 857, 909, 1118, 1133
NmuCI GTSAC 2 cut(s) 899, 998
NsbI TGCGCA 1 cut(s) 1290
NspI RCATGY 1 cut(s) 1265
PaeR7I CTCGAG 3 cut(s) 532, 1299, 1319
PagI TCATGA 1 cut(s) 1390
PceI AGGCCT 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 1307
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 284
PctI GAATGC 2 cut(s) 935, 1151
PdiI GCCGGC 2 cut(s) 301, 835
PfeI GAWTC 6 cut(s) 562, 578, 634, 778, 1026, 1164
PfoI TCCNGGA 2 cut(s) 351, 732
PleI GAGTC 2 cut(s) 752, 766
PmaCI CACGTG 2 cut(s) 391, 805
PmlI CACGTG 2 cut(s) 391, 805
PpsI GAGTC 2 cut(s) 752, 766
Ppu21I YACGTR 2 cut(s) 391, 805
Psp124BI GAGCTC 2 cut(s) 42, 483
Psp6I CCWGG 2 cut(s) 303, 334
PspCI CACGTG 2 cut(s) 391, 805
PspGI CCWGG 2 cut(s) 303, 334
PspN4I GGNNCC 7 cut(s) 56, 384, 419, 857, 909, 1118, 1133
PspOMI GGGCCC 1 cut(s) 382
PspPI GGNCC 8 cut(s) 55, 338, 382, 383, 417, 856, 1117, 1131
PspXI VCTCGAGB 2 cut(s) 1299, 1319
PsuI RGATCY 1 cut(s) 423
PvuII CAGCTG 2 cut(s) 989, 1204
RigI GGCCGGCC 1 cut(s) 303
RsaI GTAC 1 cut(s) 1266
RsaNI GTAC 1 cut(s) 1265
SacI GAGCTC 2 cut(s) 42, 483
SapI GCTCTTC 1 cut(s) 1307
SaqAI TTAA 3 cut(s) 954, 1004, 1356
Sau3AI GATC 5 cut(s) 223, 423, 473, 540, 1348
Sau96I GGNCC 8 cut(s) 55, 338, 382, 383, 417, 856, 1117, 1131
SchI GAGTC 2 cut(s) 752, 766
SduI GDGCHC 7 cut(s) 42, 386, 470, 483, 912, 1201, 1300
SfaNI GCATC 3 cut(s) 270, 346, 1210
Sfr274I CTCGAG 3 cut(s) 532, 1299, 1319
SinI GGWCC 5 cut(s) 55, 417, 856, 1117, 1131
SlaI CTCGAG 3 cut(s) 532, 1299, 1319
SmlI CTYRAG 3 cut(s) 532, 1299, 1319
SmoI CTYRAG 3 cut(s) 532, 1299, 1319
Sse9I AATT 3 cut(s) 727, 738, 1006
SseBI AGGCCT 1 cut(s) 275
SspMI CTAG 1 cut(s) 330
SstI GAGCTC 2 cut(s) 42, 483
StuI AGGCCT 1 cut(s) 275
TaaI ACNGT 2 cut(s) 247, 1196
TaiI ACGT 8 cut(s) 27, 261, 270, 348, 393, 747, 807, 852
TasI AATT 3 cut(s) 727, 738, 1006
TatI WGTACW 1 cut(s) 1264
TauI GCSGC 8 cut(s) 69, 343, 651, 791, 877, 890, 1052, 1237
TfiI GAWTC 6 cut(s) 562, 578, 634, 778, 1026, 1164
Tru1I TTAA 3 cut(s) 954, 1004, 1356
Tru9I TTAA 3 cut(s) 954, 1004, 1356
TscAI CASTG 2 cut(s) 120, 1201
TseFI GTSAC 2 cut(s) 899, 998
TseI GCWGC 7 cut(s) 283, 490, 791, 986, 1148, 1183, 1237
Tsp45I GTSAC 2 cut(s) 899, 998
TspDTI ATGAA 2 cut(s) 707, 1407
TspGWI ACGGA 2 cut(s) 97, 500
TspRI CASTG 2 cut(s) 120, 1201
VpaK11BI GGWCC 5 cut(s) 55, 417, 856, 1117, 1131
XapI RAATTY 2 cut(s) 727, 1006
XceI RCATGY 1 cut(s) 1265
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XhoI CTCGAG 3 cut(s) 532, 1299, 1319
XspI CTAG 1 cut(s) 330
ZraI GACGTC 2 cut(s) 259, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.