MD03G1273500.v1.1

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015548
Physical Location & Seq
Forward (+)
35689228 .. 35690670
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1273500.v1.1.491

Sequence Viewer

Length: 1443 bp
ATGGACAGGCTGGTGAAGCCAGACGTGAAGGAAGTGGAGCTCAACTTCAAGAGAGACCAAAAGTGCAGCACAACCTTCCGGCTGAGCAACCTCATGCACACCATGCCCGTCGCCGTCTCCCTCACCACCAACAACCCATCTCTCTTTTCCTTCACTCACCCCCTCTCCATAATCCCGCCGCTCTCTTCCTCCTCCTACACCCTCCTCCTCTCACAGCCGTCCGATGAGCCCCCGATATCCCTCACGGCCACCCCTCATGACGTCATCAACGTCAAGGCCTCCATGCTCCCCACCGGAAAAGCCCACCAGGAGGAACTCCGCCGCCTGTTCGCCCGGCCCGGGCCTCACGTTTTCCGGGACGCCACCATACCCATATCGTTCGTGGGTCCGCACGTGGTTGAGTTTCTGATTTCTCAGCACACCCGGATCTCGGCATTCGATTCGTTTTTCAACAAGGCGATTTCTGGGTGCTCTGGGGCTGAGCTCACGGCGCTGCTCCGTCCCGCCATAGCTTCCGGGAATGCTAATTTGGTCTCCGACTTGATTGAAGCCGGTGCGGTTGTGAATCGGAGAGATTCGGATTCCGGATCTTTGCTATCTCTCTGTGTTCGAACCGGAAACGTTGAGATTGTAAAGGTCCTGATCGCCTCTGGATGTGGAATCGGTAACTCGGCCGACATGGTTTTGCACGACGCGGCGGCGATGAACCGGGTCGATTTGTTTGAGATTTTGTGTAAGTGTTTCACCGGAATCGACGTCAATTGTGTTGATCCGGACGGTCGGACTCCCATTCATATCGCGGCGGCGCACGGCCACGTTGAGATGTTGAAGTATTGTATTTCGGTCGGAGGCAATGCGGAAGTTTCAGACTCAAAAGGTTGGAATCCGCTTCACTGCGCGGCGGAGAAGGGGCATTTGGAGGCCGCCAAGTGTTTGTTGACGTGCTCCAAGTTGAAATACGCCGTCACGAAAGACGGAAGGACGGCTTTCGACCTCGCCGCCACAAACGGGCATTCCTGTCTTCTCGGTTGTCTACGATACGACGACGTTCTGCACCGGACGGCGAGGTCGGACGACGCACACGGGATAAAGAGTTGCCTTGCGGAAGGGGCAGACGTGAACGGGAGAGACCAGAATGGCTGGACCCCTTTGCACAGGGCGGCGTTCAAAGGGAGGATCGAGTGTGTGAAGGTGTTGCTTAATCATGGTGCGGTGGTGGATGCGGTGGATGATGTCGGCTACACGCCGCTGCATTGCGCGGCGGAGGCGGGGCACGTGCAGGTGGCTCTGTTGCTGATTGCTCATGGAGCTCGAGCCAACGTGAAGAGCTTGGAGGGAATTGTTCCTACCAATTTGGACCGTTTCAAGACCCACCCTTCTCTGATTTCACATAACGAAAATAACGTGCAACTGAAACGTTTTTTTGACGATATCTCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001508 GO:0002027 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003205 GO:0003230 GO:0003279 GO:0003283 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0006888 GO:0006928 GO:0006936 GO:0006937 GO:0006941 GO:0006942 GO:0007009 GO:0007154 GO:0007165 GO:0007267 GO:0007275 GO:0007507 GO:0008015 GO:0008016 GO:0008092 GO:0008104 GO:0008150 GO:0009893 GO:0009987 GO:0010256 GO:0010468 GO:0010522 GO:0010604 GO:0010628 GO:0010646 GO:0010880 GO:0010881 GO:0010882 GO:0010959 GO:0014704 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0019222 GO:0019722 GO:0019725 GO:0019899 GO:0019900 GO:0019901 GO:0019932 GO:0022898 GO:0023051 GO:0023052 GO:0030001 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030048 GO:0030054 GO:0030154 GO:0030315 GO:0030507 GO:0030674 GO:0031430 GO:0031647 GO:0031672 GO:0031674 GO:0032409 GO:0032411 GO:0032412 GO:0032414 GO:0032501 GO:0032502 GO:0032879 GO:0032970 GO:0033036 GO:0033292 GO:0033365 GO:0034394 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035556 GO:0035637 GO:0036309 GO:0036371 GO:0042383 GO:0042391 GO:0042592 GO:0042692 GO:0043034 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043292 GO:0044057 GO:0044093 GO:0044291 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044459 GO:0044464 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050821 GO:0050896 GO:0051049 GO:0051050 GO:0051117 GO:0051179 GO:0051234 GO:0051239 GO:0051270 GO:0051279 GO:0051282 GO:0051641 GO:0051649 GO:0051716 GO:0051899 GO:0051924 GO:0051928 GO:0055001 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055117 GO:0060047 GO:0060048 GO:0060090 GO:0060255 GO:0060306 GO:0060307 GO:0060341 GO:0061024 GO:0061061 GO:0061337 GO:0065007 GO:0065008 GO:0065009 GO:0070252 GO:0070296 GO:0070727 GO:0070838 GO:0070972 GO:0071840 GO:0071944 GO:0072359 GO:0072503 GO:0072507 GO:0072511 GO:0072657 GO:0072659 GO:0086001 GO:0086002 GO:0086003 GO:0086004 GO:0086005 GO:0086010 GO:0086012 GO:0086014 GO:0086015 GO:0086018 GO:0086019 GO:0086026 GO:0086046 GO:0086065 GO:0086066 GO:0086070 GO:0086091 GO:0090257 GO:0098590 GO:0098771 GO:0098900 GO:0098901 GO:0098907 GO:0098910 GO:0099080 GO:0099081 GO:0099512 GO:0099623 GO:1901016 GO:1901018 GO:1901019 GO:1901021 GO:1901379 GO:1901381 GO:1903115 GO:1903169 GO:1903522 GO:1903779 GO:1904062 GO:1904064 GO:1904427 GO:1990778 GO:2001257 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4

Protein Analysis

481

Amino Acids

51.7

Weight (kDa)

6.63

Isoelectric Point (pI)

25.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 169 - 256 1e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 229 - 314 5.1e-18 Ankyrin repeats (3 copies)
Ank_4 PF13637 229 - 278 9.4e-11 Ankyrin repeats (many copies)
Ank_5 PF13857 247 - 297 2.1e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 285 - 339 1.3e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 351 - 442 4.5e-20 Ankyrin repeats (3 copies)
Ank_4 PF13637 363 - 400 1.6e-08 Ankyrin repeats (many copies)
Ank PF00023 379 - 411 6.3e-09 Ankyrin repeat
Ank_4 PF13637 384 - 433 1.2e-12 Ankyrin repeats (many copies)
Ank PF00023 413 - 442 1.3e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015548)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24420
malus_domestica MD03G1273500.v1.1 MD11G1296100.v1.1
prunus_persica Prupe.8G249900_v2.0.a1
pyrus_communis pycom03g21790 pycom11g26060
rosa_chinensis RchiOBHm_Chr6g0292251
rosa_laevigata RLG00000012048
rosa_multiflora Rmu_sc0002993.1_g000015
rosa_roxburghii Rroxscaffold_7G00175280
rosa_rugosa Rorug06G0224900
rosa_samantha Rh6AG338200 Rh6BG345500 Rh6CG352400 Rh6DG338400
rosa_wichuraiana Rw6G029430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1270
AasI GACNNNNNNGTC 1 cut(s) 1066
AatII GACGTC 2 cut(s) 264, 759
Acc36I ACCTGC 1 cut(s) 1270
AccBSI CCGCTC 1 cut(s) 181
AccI GTMKAC 1 cut(s) 1033
AccII CGCG 4 cut(s) 695, 800, 899, 1259
AccIII TCCGGA 2 cut(s) 584, 772
AclI AACGTT 2 cut(s) 621, 1417
AclWI GGATC 4 cut(s) 434, 595, 764, 1184
AcoI YGGCCR 3 cut(s) 246, 672, 811
AcvI CACGTG 2 cut(s) 394, 1276
AcyI GRCGYC 3 cut(s) 261, 360, 756
AfiI CCNNNNNNNGG 4 cut(s) 310, 339, 430, 1008
AgsI TTSAA 7 cut(s) 49, 451, 548, 829, 955, 1168, 1366
AjiI CACGTC 3 cut(s) 25, 942, 1117
AjnI CCWGG 1 cut(s) 306
AjuI GAANNNNNNNTTGG 4 cut(s) 512, 544, 899, 931
AluBI AGCT 5 cut(s) 40, 484, 512, 1310, 1329
AluI AGCT 5 cut(s) 40, 484, 512, 1310, 1329
Alw21I GWGCWC 5 cut(s) 42, 473, 486, 947, 1312
Alw26I GTCTC 4 cut(s) 48, 121, 538, 1122
AlwI GGATC 4 cut(s) 434, 595, 764, 1184
Ama87I CYCGRG 2 cut(s) 338, 1311
Aor13HI TCCGGA 2 cut(s) 584, 772
AoxI GGCC 7 cut(s) 246, 276, 335, 341, 672, 811, 921
ApeKI GCWGC 3 cut(s) 66, 493, 1249
AspLEI GCGC 4 cut(s) 493, 808, 899, 1259
AspS9I GGNCC 6 cut(s) 336, 341, 386, 637, 1143, 1357
AsuC2I CCSGG 7 cut(s) 334, 339, 340, 356, 424, 517, 710
AsuHPI GGTGA 4 cut(s) 25, 115, 149, 736
AsuII TTCGAA 1 cut(s) 610
AvaI CYCGRG 2 cut(s) 338, 1311
AvaII GGWCC 4 cut(s) 386, 637, 1143, 1357
BaeGI GKGCMC 1 cut(s) 1275
BanII GRGCYC 4 cut(s) 42, 231, 486, 1312
BbrPI CACGTG 2 cut(s) 394, 1276
BbsI GAAGAC 1 cut(s) 1013
Bbv12I GWGCWC 5 cut(s) 42, 473, 486, 947, 1312
BbvI GCAGC 3 cut(s) 78, 480, 1236
BccI CCATC 1 cut(s) 145
BceAI ACGGC 8 cut(s) 98, 202, 261, 504, 826, 947, 999, 1077
BcgI CGANNNNNNTGC 2 cut(s) 423, 457
BciT130I CCWGG 1 cut(s) 308
BcnI CCSGG 7 cut(s) 334, 339, 340, 356, 424, 517, 710
BcoDI GTCTC 4 cut(s) 48, 121, 538, 1122
BfoI RGCGCY 1 cut(s) 494
BfuAI ACCTGC 1 cut(s) 1270
BlpI GCTNAGC 2 cut(s) 83, 480
Bme1390I CCNGG 8 cut(s) 308, 334, 339, 340, 356, 424, 517, 710
Bme18I GGWCC 4 cut(s) 386, 637, 1143, 1357
BmeT110I CYCGRG 2 cut(s) 338, 1311
BmgBI CACGTC 3 cut(s) 25, 942, 1117
BmgT120I GGNCC 6 cut(s) 336, 341, 386, 637, 1143, 1357
BmiI GGNNCC 2 cut(s) 387, 1145
BmrFI CCNGG 8 cut(s) 308, 334, 339, 340, 356, 424, 517, 710
BmsI GCATC 1 cut(s) 1210
BpiI GAAGAC 1 cut(s) 1013
Bpu1102I GCTNAGC 2 cut(s) 83, 480
Bpu14I TTCGAA 1 cut(s) 610
BpuMI CCSGG 7 cut(s) 334, 339, 340, 356, 424, 517, 710
BsaAI YACGTR 2 cut(s) 394, 1276
BsaHI GRCGYC 3 cut(s) 261, 360, 756
BsaI GGTCTC 3 cut(s) 48, 538, 1122
BsaJI CCNNGG 1 cut(s) 338
BsaWI WCCGGW 6 cut(s) 293, 584, 614, 746, 772, 1056
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 4 cut(s) 310, 339, 430, 1008
Bse118I RCCGGY 1 cut(s) 551
Bse3DI GCAATG 2 cut(s) 859, 1252
BseAI TCCGGA 2 cut(s) 584, 772
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 1 cut(s) 338
BseGI GGATG 3 cut(s) 659, 1225, 1234
BseLI CCNNNNNNNGG 4 cut(s) 310, 339, 430, 1008
BseMI GCAATG 2 cut(s) 859, 1252
BseMII CTCAG 3 cut(s) 74, 428, 471
BseRI GAGGAG 3 cut(s) 181, 194, 197
BseSI GKGCMC 1 cut(s) 1275
BseX3I CGGCCG 1 cut(s) 672
BseXI GCAGC 3 cut(s) 78, 480, 1236
BsgI GTGCAG 3 cut(s) 85, 1037, 1298
Bsh1236I CGCG 4 cut(s) 695, 800, 899, 1259
Bsh1285I CGRYCG 3 cut(s) 675, 781, 846
BshFI GGCC 7 cut(s) 248, 278, 337, 343, 674, 813, 923
BsiEI CGRYCG 3 cut(s) 675, 781, 846
BsiHKAI GWGCWC 5 cut(s) 42, 473, 486, 947, 1312
BsiHKCI CYCGRG 2 cut(s) 338, 1311
BslFI GGGAC 2 cut(s) 371, 486
BslI CCNNNNNNNGG 4 cut(s) 310, 339, 430, 1008
BsmAI GTCTC 4 cut(s) 48, 121, 538, 1122
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 2 cut(s) 371, 486
BsmI GAATGC 3 cut(s) 434, 526, 1012
BsnI GGCC 7 cut(s) 248, 278, 337, 343, 674, 813, 923
Bso31I GGTCTC 3 cut(s) 48, 538, 1122
BsoBI CYCGRG 2 cut(s) 338, 1311
Bsp119I TTCGAA 1 cut(s) 610
Bsp1286I GDGCHC 7 cut(s) 42, 231, 473, 486, 947, 1275, 1312
Bsp13I TCCGGA 2 cut(s) 584, 772
Bsp143I GATC 5 cut(s) 426, 587, 642, 769, 1176
Bsp1720I GCTNAGC 2 cut(s) 83, 480
BspANI GGCC 7 cut(s) 248, 278, 337, 343, 674, 813, 923
BspCNI CTCAG 3 cut(s) 75, 427, 472
BspEI TCCGGA 2 cut(s) 584, 772
BspFNI CGCG 4 cut(s) 695, 800, 899, 1259
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 2 cut(s) 387, 1145
BspMI ACCTGC 1 cut(s) 1270
BspPI GGATC 4 cut(s) 434, 595, 764, 1184
BspQI GCTCTTC 1 cut(s) 1319
BspT104I TTCGAA 1 cut(s) 610
BspTNI GGTCTC 3 cut(s) 48, 538, 1122
BsrBI CCGCTC 1 cut(s) 181
BsrDI GCAATG 2 cut(s) 859, 1252
BsrFI RCCGGY 1 cut(s) 551
BssAI RCCGGY 1 cut(s) 551
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 5 cut(s) 426, 587, 642, 769, 1176
BssNI GRCGYC 3 cut(s) 261, 360, 756
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 2 cut(s) 779, 1361
Bst6I CTCTTC 2 cut(s) 190, 1319
BstACI GRCGYC 3 cut(s) 261, 360, 756
BstAPI GCANNNNNTGC 1 cut(s) 103
BstBAI YACGTR 2 cut(s) 394, 1276
BstBI TTCGAA 1 cut(s) 610
BstDEI CTNAG 3 cut(s) 83, 414, 480
BstF5I GGATG 3 cut(s) 659, 1225, 1234
BstFNI CGCG 4 cut(s) 695, 800, 899, 1259
BstH2I RGCGCY 1 cut(s) 494
BstHHI GCGC 4 cut(s) 493, 808, 899, 1259
BstKTI GATC 5 cut(s) 429, 590, 645, 772, 1179
BstMAI GTCTC 4 cut(s) 48, 121, 538, 1122
BstMBI GATC 5 cut(s) 426, 587, 642, 769, 1176
BstMCI CGRYCG 3 cut(s) 675, 781, 846
BstMWI GCNNNNNNNGC 6 cut(s) 16, 103, 490, 1109, 1265, 1307
BstNI CCWGG 1 cut(s) 308
BstSCI CCNGG 8 cut(s) 306, 332, 337, 338, 354, 422, 515, 708
BstSLI GKGCMC 1 cut(s) 1275
BstUI CGCG 4 cut(s) 695, 800, 899, 1259
BstV1I GCAGC 3 cut(s) 78, 480, 1236
BstV2I GAAGAC 1 cut(s) 1013
BstX2I RGATCY 2 cut(s) 426, 587
BstYI RGATCY 2 cut(s) 426, 587
BstZI CGGCCG 1 cut(s) 672
BsuRI GGCC 7 cut(s) 248, 278, 337, 343, 674, 813, 923
BtgZI GCGATG 1 cut(s) 716
BtrI CACGTC 3 cut(s) 25, 942, 1117
BtsCI GGATG 3 cut(s) 659, 1225, 1234
BtsI GCAGTG 1 cut(s) 892
BtsIMutI CAGTG 1 cut(s) 892
BveI ACCTGC 1 cut(s) 1270
CciI TCATGA 1 cut(s) 256
CfoI GCGC 4 cut(s) 493, 808, 899, 1259
Cfr10I RCCGGY 1 cut(s) 551
Cfr13I GGNCC 6 cut(s) 336, 341, 386, 637, 1143, 1357
Cfr9I CCCGGG 1 cut(s) 338
CseI GACGC 3 cut(s) 368, 701, 1085
CviAII CATG 8 cut(s) 94, 103, 257, 283, 679, 1205, 1304, 1437
DdeI CTNAG 3 cut(s) 83, 414, 480
DpnI GATC 5 cut(s) 428, 589, 644, 771, 1178
DpnII GATC 5 cut(s) 426, 587, 642, 769, 1176
DrdI GACNNNNNNGTC 1 cut(s) 1066
DseDI GACNNNNNNGTC 1 cut(s) 1066
EaeI YGGCCR 3 cut(s) 246, 672, 811
EagI CGGCCG 1 cut(s) 672
Eam1104I CTCTTC 2 cut(s) 190, 1319
EarI CTCTTC 2 cut(s) 190, 1319
EciI GGCGGA 3 cut(s) 308, 917, 1277
Ecl136II GAGCTC 3 cut(s) 40, 484, 1310
EclXI CGGCCG 1 cut(s) 672
Eco147I AGGCCT 1 cut(s) 278
Eco24I GRGCYC 4 cut(s) 42, 231, 486, 1312
Eco31I GGTCTC 3 cut(s) 48, 538, 1122
Eco32I GATATC 2 cut(s) 237, 1432
Eco47I GGWCC 4 cut(s) 386, 637, 1143, 1357
Eco52I CGGCCG 1 cut(s) 672
Eco53kI GAGCTC 3 cut(s) 40, 484, 1310
Eco72I CACGTG 2 cut(s) 394, 1276
Eco88I CYCGRG 2 cut(s) 338, 1311
EcoICRI GAGCTC 3 cut(s) 40, 484, 1310
EcoO109I RGGNCCY 1 cut(s) 637
EcoRII CCWGG 1 cut(s) 306
EcoRV GATATC 2 cut(s) 237, 1432
EcoT38I GRGCYC 4 cut(s) 42, 231, 486, 1312
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 8 cut(s) 97, 106, 260, 286, 682, 1208, 1307, 1440
FalI AAGNNNNNCTT 2 cut(s) 970, 1002
FaqI GGGAC 2 cut(s) 371, 486
FatI CATG 8 cut(s) 93, 102, 256, 282, 678, 1204, 1303, 1436
FauI CCCGC 3 cut(s) 183, 511, 1261
FblI GTMKAC 1 cut(s) 1033
FokI GGATG 3 cut(s) 666, 1232, 1241
FriOI GRGCYC 4 cut(s) 42, 231, 486, 1312
GlaI GCGC 4 cut(s) 492, 807, 898, 1258
HaeII RGCGCY 1 cut(s) 494
HaeIII GGCC 7 cut(s) 248, 278, 337, 343, 674, 813, 923
HgaI GACGC 3 cut(s) 368, 701, 1085
HhaI GCGC 4 cut(s) 493, 808, 899, 1259
Hin1I GRCGYC 3 cut(s) 261, 360, 756
Hin1II CATG 8 cut(s) 97, 106, 260, 286, 682, 1208, 1307, 1440
Hin6I GCGC 4 cut(s) 491, 806, 897, 1257
HinP1I GCGC 4 cut(s) 491, 806, 897, 1257
HincII GTYRAC 1 cut(s) 939
HindII GTYRAC 1 cut(s) 939
HinfI GANTC 9 cut(s) 440, 565, 575, 581, 660, 750, 784, 869, 883
HphI GGTGA 4 cut(s) 25, 115, 149, 736
Hpy166II GTNNAC 3 cut(s) 939, 1034, 1120
Hpy188III TCNNGA 8 cut(s) 49, 257, 585, 640, 651, 773, 967, 1366
Hpy8I GTNNAC 3 cut(s) 939, 1034, 1120
Hpy99I CGWCG 6 cut(s) 113, 695, 758, 1046, 1049, 1079
HpyAV CCTTC 8 cut(s) 22, 85, 160, 901, 972, 1100, 1183, 1386
HpyCH4III ACNGT 2 cut(s) 779, 1361
HpyCH4V TGCA 8 cut(s) 66, 97, 688, 1054, 1153, 1252, 1279, 1408
HpyF10VI GCNNNNNNNGC 6 cut(s) 16, 103, 490, 1109, 1265, 1307
HpyF3I CTNAG 3 cut(s) 83, 414, 480
Hsp92I GRCGYC 3 cut(s) 261, 360, 756
Hsp92II CATG 8 cut(s) 97, 106, 260, 286, 682, 1208, 1307, 1440
HspAI GCGC 4 cut(s) 491, 806, 897, 1257
Kpn2I TCCGGA 2 cut(s) 584, 772
Kzo9I GATC 5 cut(s) 426, 587, 642, 769, 1176
LguI GCTCTTC 1 cut(s) 1319
LmnI GCTCC 5 cut(s) 37, 291, 501, 950, 1307
Lsp1109I GCAGC 3 cut(s) 78, 480, 1236
LweI GCATC 1 cut(s) 1210
MaeIII GTNAC 2 cut(s) 665, 964
MalI GATC 5 cut(s) 428, 589, 644, 771, 1178
MbiI CCGCTC 1 cut(s) 181
MboI GATC 5 cut(s) 426, 587, 642, 769, 1176
MboII GAAGA 3 cut(s) 177, 1013, 1336
MfeI CAATTG 1 cut(s) 760
MflI RGATCY 2 cut(s) 426, 587
MhlI GDGCHC 7 cut(s) 42, 231, 473, 486, 947, 1275, 1312
MluCI AATT 4 cut(s) 526, 760, 1338, 1351
MlyI GAGTC 2 cut(s) 778, 863
MmeI TCCRAC 5 cut(s) 561, 761, 826, 860, 1050
MroI TCCGGA 2 cut(s) 584, 772
MseI TTAA 1 cut(s) 1200
MspA1I CMGCKG 1 cut(s) 1249
MspR9I CCNGG 8 cut(s) 308, 334, 339, 340, 356, 424, 517, 710
MunI CAATTG 1 cut(s) 760
Mva1269I GAATGC 3 cut(s) 434, 526, 1012
MvaI CCWGG 1 cut(s) 308
MvnI CGCG 4 cut(s) 695, 800, 899, 1259
MwoI GCNNNNNNNGC 6 cut(s) 16, 103, 490, 1109, 1265, 1307
NciI CCSGG 7 cut(s) 334, 339, 340, 356, 424, 517, 710
NdeII GATC 5 cut(s) 426, 587, 642, 769, 1176
NlaIII CATG 8 cut(s) 97, 106, 260, 286, 682, 1208, 1307, 1440
NlaIV GGNNCC 2 cut(s) 387, 1145
NmeAIII GCCGAG 2 cut(s) 410, 650
NmuCI GTSAC 1 cut(s) 964
NspV TTCGAA 1 cut(s) 610
PaeR7I CTCGAG 1 cut(s) 1311
PagI TCATGA 1 cut(s) 256
PaqCI CACCTGC 1 cut(s) 1270
PceI AGGCCT 1 cut(s) 278
PciSI GCTCTTC 1 cut(s) 1319
PcsI WCGNNNNNNNCGW 2 cut(s) 267, 1401
PctI GAATGC 3 cut(s) 434, 526, 1012
PfeI GAWTC 7 cut(s) 440, 565, 575, 581, 660, 750, 883
PfoI TCCNGGA 2 cut(s) 354, 515
PleI GAGTC 2 cut(s) 778, 863
PmaCI CACGTG 2 cut(s) 394, 1276
PmlI CACGTG 2 cut(s) 394, 1276
PpsI GAGTC 2 cut(s) 778, 863
Ppu21I YACGTR 2 cut(s) 394, 1276
PpuMI RGGWCCY 1 cut(s) 637
Psp124BI GAGCTC 3 cut(s) 42, 486, 1312
Psp1406I AACGTT 2 cut(s) 621, 1417
Psp5II RGGWCCY 1 cut(s) 637
Psp6I CCWGG 1 cut(s) 306
PspCI CACGTG 2 cut(s) 394, 1276
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 2 cut(s) 387, 1145
PspPI GGNCC 6 cut(s) 336, 341, 386, 637, 1143, 1357
PspPPI RGGWCCY 1 cut(s) 637
PspXI VCTCGAGB 1 cut(s) 1311
PsuI RGATCY 2 cut(s) 426, 587
SacI GAGCTC 3 cut(s) 42, 486, 1312
SapI GCTCTTC 1 cut(s) 1319
SaqAI TTAA 1 cut(s) 1200
Sau3AI GATC 5 cut(s) 426, 587, 642, 769, 1176
Sau96I GGNCC 6 cut(s) 336, 341, 386, 637, 1143, 1357
SchI GAGTC 2 cut(s) 778, 863
ScrFI CCNGG 8 cut(s) 308, 334, 339, 340, 356, 424, 517, 710
SduI GDGCHC 7 cut(s) 42, 231, 473, 486, 947, 1275, 1312
SfaNI GCATC 1 cut(s) 1210
Sfr274I CTCGAG 1 cut(s) 1311
SfuI TTCGAA 1 cut(s) 610
SinI GGWCC 4 cut(s) 386, 637, 1143, 1357
SlaI CTCGAG 1 cut(s) 1311
SmaI CCCGGG 1 cut(s) 340
SmlI CTYRAG 1 cut(s) 1311
SmoI CTYRAG 1 cut(s) 1311
SrfI GCCCGGGC 1 cut(s) 340
Sse9I AATT 4 cut(s) 526, 760, 1338, 1351
SseBI AGGCCT 1 cut(s) 278
SstI GAGCTC 3 cut(s) 42, 486, 1312
StuI AGGCCT 1 cut(s) 278
StyD4I CCNGG 8 cut(s) 306, 332, 337, 338, 354, 422, 515, 708
TaaI ACNGT 2 cut(s) 779, 1361
TaqI TCGA 7 cut(s) 438, 610, 714, 753, 990, 1179, 1312
TaqII GACCGA 1 cut(s) 832
TasI AATT 4 cut(s) 526, 760, 1338, 1351
TfiI GAWTC 7 cut(s) 440, 565, 575, 581, 660, 750, 883
Tru1I TTAA 1 cut(s) 1200
Tru9I TTAA 1 cut(s) 1200
TscAI CASTG 1 cut(s) 899
TseFI GTSAC 1 cut(s) 964
TseI GCWGC 3 cut(s) 66, 493, 1249
Tsp45I GTSAC 1 cut(s) 964
TspDTI ATGAA 2 cut(s) 719, 782
TspGWI ACGGA 2 cut(s) 488, 990
TspMI CCCGGG 1 cut(s) 338
TspRI CASTG 1 cut(s) 899
VpaK11BI GGWCC 4 cut(s) 386, 637, 1143, 1357
XcmI CCANNNNNNNNNTGG 1 cut(s) 379
XhoI CTCGAG 1 cut(s) 1311
XmaI CCCGGG 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 1033
ZraI GACGTC 2 cut(s) 262, 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.