FvH4_3g00481

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0017615
Physical Location & Seq
Forward (+)
255686 .. 256263
578 bp
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UTR
Exon/CDS
Intron
FvH4_3g00481.t1

Sequence Viewer

Length: 465 bp
ATGGGAAACTGTATCTGTGCTCCGTTCCATCCGCCCCAAGAGAAGATCAAGGTAGTAGTCTTTAATGGAGGAGTAGAAGAGTTCAGTGCCTCGACAACAGTTGAAGAAATCACATCCGGCCCTTACGTGGATTACAAGCTGGTGCACAAGGCCAACCCATATACACCATTGTCACCCAGCACCGACCTAGAACCTGGGGAGGTTTACCATCTTGTGCCAAACCTTGTATGGCTCAACAAGGCTTTGGTTCCTTCAAAGATAGCTCACCAGGAACAAGAAAGCGGCAAAAGACAGAAGGTGAGGATAGTGGTGACTAGAGAGCAATTGGAGCAGTTGTTGAATGGCAAAAATAAGATCGGGTCTTTGAATATTGGGAGTTTTGGGTCACGAGAGCCTCGCAAATGGAGGCCTTCTTTGGCTATGATCCCAGAGATGATTGTAGAGGGAGGGGTACATGGATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

155

Amino Acids

17.05

Weight (kDa)

7.81

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 144 7.5e-16 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017615)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00481
prunus_persica Prupe.4G005300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0000641
rosa_laevigata RLG00000008373 RLG00000030884
rosa_multiflora Rmu_sc0011992.1_g000013
rosa_roxburghii Rroxscaffold_1G00075560
rosa_samantha Rh5AG005500 Rh5BG007100 Rh5CG005800 Rh5DG005700
rosa_wichuraiana Rw5G000540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 32, 282
AclWI GGATC 1 cut(s) 418
AfaI GTAC 1 cut(s) 453
AfiI CCNNNNNNNGG 1 cut(s) 127
AgsI TTSAA 4 cut(s) 104, 255, 340, 367
AjnI CCWGG 2 cut(s) 193, 267
AluBI AGCT 2 cut(s) 139, 263
AluI AGCT 2 cut(s) 139, 263
Alw21I GWGCWC 2 cut(s) 22, 147
Alw44I GTGCAC 1 cut(s) 143
AlwI GGATC 1 cut(s) 418
AoxI GGCC 3 cut(s) 118, 150, 407
ApaLI GTGCAC 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 4 cut(s) 165, 257, 310, 322
BaeGI GKGCMC 1 cut(s) 147
BauI CACGAG 1 cut(s) 387
Bbv12I GWGCWC 2 cut(s) 22, 147
BccI CCATC 2 cut(s) 36, 216
BciT130I CCWGG 2 cut(s) 195, 269
BfaI CTAG 2 cut(s) 188, 315
BisI GCNGC 1 cut(s) 283
BlsI GCNGC 1 cut(s) 284
Bme1390I CCNGG 2 cut(s) 195, 269
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 2 cut(s) 195, 269
BsaAI YACGTR 1 cut(s) 127
BsaJI CCNNGG 1 cut(s) 194
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 127
BseBI CCWGG 2 cut(s) 195, 269
BseDI CCNNGG 1 cut(s) 194
BseGI GGATG 2 cut(s) 28, 113
BseLI CCNNNNNNNGG 1 cut(s) 127
BseRI GAGGAG 1 cut(s) 84
BseSI GKGCMC 1 cut(s) 147
BseYI CCCAGC 1 cut(s) 176
BshFI GGCC 3 cut(s) 120, 152, 409
BsiHKAI GWGCWC 2 cut(s) 22, 147
BsiSI CCGG 1 cut(s) 117
BslI CCNNNNNNNGG 1 cut(s) 127
BsnI GGCC 3 cut(s) 120, 152, 409
Bsp1286I GDGCHC 2 cut(s) 22, 147
Bsp143I GATC 3 cut(s) 45, 354, 423
BspACI CCGC 2 cut(s) 32, 282
BspANI GGCC 3 cut(s) 120, 152, 409
BspLI GGNNCC 1 cut(s) 249
BspPI GGATC 1 cut(s) 418
BssECI CCNNGG 1 cut(s) 194
BssMI GATC 3 cut(s) 45, 354, 423
BssSI CACGAG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 387
Bst2UI CCWGG 2 cut(s) 195, 269
Bst4CI ACNGT 2 cut(s) 11, 100
Bst6I CTCTTC 1 cut(s) 72
BstBAI YACGTR 1 cut(s) 127
BstF5I GGATG 2 cut(s) 28, 113
BstKTI GATC 3 cut(s) 48, 357, 426
BstMBI GATC 3 cut(s) 45, 354, 423
BstMWI GCNNNNNNNGC 1 cut(s) 328
BstNI CCWGG 2 cut(s) 195, 269
BstSCI CCNGG 2 cut(s) 193, 267
BstSLI GKGCMC 1 cut(s) 147
BsuRI GGCC 3 cut(s) 120, 152, 409
BtsCI GGATG 2 cut(s) 28, 113
BtsIMutI CAGTG 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 119
Csp6I GTAC 1 cut(s) 452
CviAII CATG 1 cut(s) 455
CviJI RGCY 9 cut(s) 120, 139, 152, 232, 242, 263, 394, 409, 419
CviKI_1 RGCY 9 cut(s) 120, 139, 152, 232, 242, 263, 394, 409, 419
CviQI GTAC 1 cut(s) 452
DpnI GATC 3 cut(s) 47, 356, 425
DpnII GATC 3 cut(s) 45, 354, 423
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
EciI GGCGGA 1 cut(s) 21
Eco147I AGGCCT 1 cut(s) 409
EcoRII CCWGG 2 cut(s) 193, 267
FaeI CATG 1 cut(s) 458
FaiI YATR 6 cut(s) 160, 162, 229, 422, 456, 463
FatI CATG 1 cut(s) 454
Fnu4HI GCNGC 1 cut(s) 283
FokI GGATG 2 cut(s) 15, 100
Fsp4HI GCNGC 1 cut(s) 283
FspBI CTAG 2 cut(s) 188, 315
GluI GCNGC 1 cut(s) 283
GsaI CCCAGC 1 cut(s) 180
HaeIII GGCC 3 cut(s) 120, 152, 409
HapII CCGG 1 cut(s) 117
Hin1II CATG 1 cut(s) 458
HpaII CCGG 1 cut(s) 117
HphI GGTGA 4 cut(s) 165, 257, 310, 322
Hpy166II GTNNAC 2 cut(s) 145, 205
Hpy188III TCNNGA 1 cut(s) 387
Hpy8I GTNNAC 2 cut(s) 145, 205
HpyAV CCTTC 3 cut(s) 261, 289, 420
HpyCH4III ACNGT 2 cut(s) 11, 100
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 1 cut(s) 145
HpyF10VI GCNNNNNNNGC 1 cut(s) 328
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 1 cut(s) 458
Kzo9I GATC 3 cut(s) 45, 354, 423
LmnI GCTCC 2 cut(s) 25, 328
LpnPI CCDG 8 cut(s) 125, 130, 180, 190, 207, 254, 281, 441
MaeI CTAG 2 cut(s) 188, 315
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 3 cut(s) 171, 310, 384
MalI GATC 3 cut(s) 47, 356, 425
MboI GATC 3 cut(s) 45, 354, 423
MboII GAAGA 3 cut(s) 55, 89, 116
MfeI CAATTG 1 cut(s) 323
MhlI GDGCHC 2 cut(s) 22, 147
MluCI AATT 1 cut(s) 323
MnlI CCTC 8 cut(s) 62, 100, 193, 294, 399, 405, 436, 440
MseI TTAA 1 cut(s) 63
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 2 cut(s) 195, 269
MunI CAATTG 1 cut(s) 323
MvaI CCWGG 2 cut(s) 195, 269
MwoI GCNNNNNNNGC 1 cut(s) 328
NdeII GATC 3 cut(s) 45, 354, 423
NlaIII CATG 1 cut(s) 458
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 3 cut(s) 171, 310, 384
PceI AGGCCT 1 cut(s) 409
PkrI GCNGC 1 cut(s) 284
Ppu21I YACGTR 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 193, 267
PspFI CCCAGC 1 cut(s) 176
PspGI CCWGG 2 cut(s) 193, 267
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 119
RsaI GTAC 1 cut(s) 453
RsaNI GTAC 1 cut(s) 452
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 1 cut(s) 283
Sau3AI GATC 3 cut(s) 45, 354, 423
Sau96I GGNCC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 195, 269
SduI GDGCHC 2 cut(s) 22, 147
SetI ASST 9 cut(s) 54, 129, 141, 189, 196, 204, 225, 265, 300
Sse9I AATT 1 cut(s) 323
SseBI AGGCCT 1 cut(s) 409
SsiI CCGC 2 cut(s) 32, 282
SspI AATATT 1 cut(s) 370
SspMI CTAG 2 cut(s) 188, 315
StuI AGGCCT 1 cut(s) 409
StyD4I CCNGG 2 cut(s) 193, 267
TaaI ACNGT 2 cut(s) 11, 100
TaiI ACGT 1 cut(s) 129
TaqI TCGA 1 cut(s) 92
TasI AATT 1 cut(s) 323
TauI GCSGC 1 cut(s) 285
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 3 cut(s) 171, 310, 384
Tsp45I GTSAC 3 cut(s) 171, 310, 384
TspGWI ACGGA 1 cut(s) 12
TspRI CASTG 1 cut(s) 91
VneI GTGCAC 1 cut(s) 143
XcmI CCANNNNNNNNNTGG 1 cut(s) 225
XspI CTAG 2 cut(s) 188, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.