Prupe.4G005300_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0017615
Physical Location & Seq
Forward (+)
314072 .. 314530
459 bp
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UTR
Exon/CDS
Intron
Prupe.4G005300.1

Sequence Viewer

Length: 459 bp
ATGGGAAACTGCTTACATGTTCTGTGCCATCCCCCTCGAGAGAAGATTAAAGTAGTAATCTTTAAGGGTGGAGTACTAGAATTCAAAGCCTCGACACCGATCGAAGAAATCACATCTGGCCCTTACATTGGCTATAAGCTGGTACACCACGTCCGGCCGTATACGCCATTGCCACCCAACACCAAGCTAGAACAAGGGGAAGTTTACCATCTTGTGCCAAACCTTGCAAGTCTTGGTAAGCCCTTGGTTCCTTCAAAAATAGTTCACCAAGAAAGCTGCAAGAGACAGAAGATTAAGATTGTGGTGACTAGGGAACAGTTGGAGTTGTTGTTGAAGAGCGCAAACAAGTTCCGGTCGCCTAAAGAAATTGGTGTTCAATTGTCAGGGAGGTCTGGGTTAGAAGGGTCTCCAAAATGGCGGCCTTCTTTGGCCATCATTCCGGAGGTGCACAGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

153

Amino Acids

16.98

Weight (kDa)

9.71

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017615)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00481
prunus_persica Prupe.4G005300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0000641
rosa_laevigata RLG00000008373 RLG00000030884
rosa_multiflora Rmu_sc0011992.1_g000013
rosa_roxburghii Rroxscaffold_1G00075560
rosa_samantha Rh5AG005500 Rh5BG007100 Rh5CG005800 Rh5DG005700
rosa_wichuraiana Rw5G000540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 161
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 1 cut(s) 418
AcoI YGGCCR 2 cut(s) 155, 429
AcsI RAATTY 1 cut(s) 80
AfaI GTAC 2 cut(s) 75, 144
AfiI CCNNNNNNNGG 1 cut(s) 128
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 4 cut(s) 85, 255, 334, 377
AjiI CACGTC 1 cut(s) 151
AluBI AGCT 3 cut(s) 139, 187, 276
AluI AGCT 3 cut(s) 139, 187, 276
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 2 cut(s) 277, 411
Alw44I GTGCAC 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 36
Aor13HI TCCGGA 1 cut(s) 439
AoxI GGCC 4 cut(s) 118, 155, 419, 429
ApaLI GTGCAC 1 cut(s) 446
ApeKI GCWGC 1 cut(s) 276
ApoI RAATTY 1 cut(s) 80
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 257, 316
AvaI CYCGRG 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 450
BalI TGGCCA 1 cut(s) 431
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 1 cut(s) 263
BccI CCATC 3 cut(s) 36, 216, 440
BceAI ACGGC 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 277, 411
BfaI CTAG 3 cut(s) 77, 188, 309
BisI GCNGC 2 cut(s) 277, 419
BlsI GCNGC 2 cut(s) 278, 420
BmcAI AGTACT 1 cut(s) 75
BmeT110I CYCGRG 1 cut(s) 36
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 411
BsaJI CCNNGG 1 cut(s) 243
BsaWI WCCGGW 2 cut(s) 351, 439
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 167
BseAI TCCGGA 1 cut(s) 439
BseDI CCNNGG 1 cut(s) 243
BseGI GGATG 1 cut(s) 28
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMI GCAATG 1 cut(s) 167
BseSI GKGCMC 1 cut(s) 450
BseX3I CGGCCG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 263
Bsh1285I CGRYCG 3 cut(s) 102, 158, 356
BshFI GGCC 4 cut(s) 120, 157, 421, 431
BsiEI CGRYCG 3 cut(s) 102, 158, 356
BsiHKAI GWGCWC 1 cut(s) 450
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 3 cut(s) 154, 352, 440
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 2 cut(s) 277, 411
BsnI GGCC 4 cut(s) 120, 157, 421, 431
Bso31I GGTCTC 1 cut(s) 411
BsoBI CYCGRG 1 cut(s) 36
Bsp1286I GDGCHC 1 cut(s) 450
Bsp13I TCCGGA 1 cut(s) 439
Bsp143I GATC 1 cut(s) 99
BspACI CCGC 1 cut(s) 418
BspANI GGCC 4 cut(s) 120, 157, 421, 431
BspEI TCCGGA 1 cut(s) 439
BspLI GGNNCC 1 cut(s) 249
BspQI GCTCTTC 1 cut(s) 329
BspTNI GGTCTC 1 cut(s) 411
BsrDI GCAATG 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 243
BssMI GATC 1 cut(s) 99
BssNAI GTATAC 1 cut(s) 162
BssT1I CCWWGG 1 cut(s) 243
Bst1107I GTATAC 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 318, 452
Bst6I CTCTTC 1 cut(s) 329
BstF5I GGATG 1 cut(s) 28
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 1 cut(s) 102
BstMAI GTCTC 2 cut(s) 277, 411
BstMBI GATC 1 cut(s) 99
BstMCI CGRYCG 3 cut(s) 102, 158, 356
BstMWI GCNNNNNNNGC 1 cut(s) 163
BstNSI RCATGY 1 cut(s) 20
BstSLI GKGCMC 1 cut(s) 450
BstV1I GCAGC 1 cut(s) 263
BstZ17I GTATAC 1 cut(s) 162
BstZI CGGCCG 1 cut(s) 155
BsuRI GGCC 4 cut(s) 120, 157, 421, 431
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 1 cut(s) 28
CfoI GCGC 1 cut(s) 341
Cfr13I GGNCC 1 cut(s) 119
Csp6I GTAC 2 cut(s) 74, 143
CviAII CATG 1 cut(s) 17
CviQI GTAC 2 cut(s) 74, 143
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
EaeI YGGCCR 2 cut(s) 155, 429
EagI CGGCCG 1 cut(s) 155
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
EclXI CGGCCG 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 243
Eco31I GGTCTC 1 cut(s) 411
Eco52I CGGCCG 1 cut(s) 155
Eco88I CYCGRG 1 cut(s) 36
EcoRI GAATTC 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 1 cut(s) 20
FaiI YATR 3 cut(s) 18, 135, 162
FatI CATG 1 cut(s) 16
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 2 cut(s) 277, 419
FokI GGATG 1 cut(s) 15
Fsp4HI GCNGC 2 cut(s) 277, 419
FspBI CTAG 3 cut(s) 77, 188, 309
GlaI GCGC 1 cut(s) 340
GluI GCNGC 2 cut(s) 277, 419
HaeIII GGCC 4 cut(s) 120, 157, 421, 431
HapII CCGG 3 cut(s) 154, 352, 440
HhaI GCGC 1 cut(s) 341
Hin1II CATG 1 cut(s) 20
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HpaII CCGG 3 cut(s) 154, 352, 440
HphI GGTGA 2 cut(s) 257, 316
Hpy166II GTNNAC 5 cut(s) 145, 162, 205, 265, 448
Hpy188III TCNNGA 2 cut(s) 38, 440
Hpy8I GTNNAC 5 cut(s) 145, 162, 205, 265, 448
HpyAV CCTTC 3 cut(s) 261, 395, 432
HpyCH4III ACNGT 2 cut(s) 318, 452
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 3 cut(s) 227, 279, 448
HpyF10VI GCNNNNNNNGC 1 cut(s) 163
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 1 cut(s) 20
HspAI GCGC 1 cut(s) 339
Kpn2I TCCGGA 1 cut(s) 439
Kzo9I GATC 1 cut(s) 99
LguI GCTCTTC 1 cut(s) 329
LpnPI CCDG 7 cut(s) 102, 125, 167, 365, 369, 378, 453
Lsp1109I GCAGC 1 cut(s) 263
MaeI CTAG 3 cut(s) 77, 188, 309
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 304
MalI GATC 1 cut(s) 101
MboI GATC 1 cut(s) 99
MboII GAAGA 4 cut(s) 55, 116, 301, 346
MfeI CAATTG 1 cut(s) 377
MhlI GDGCHC 1 cut(s) 450
MlsI TGGCCA 1 cut(s) 431
MluCI AATT 3 cut(s) 80, 366, 377
MluNI TGGCCA 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 4 cut(s) 45, 100, 381, 436
Mox20I TGGCCA 1 cut(s) 431
MroI TCCGGA 1 cut(s) 439
MscI TGGCCA 1 cut(s) 431
MseI TTAA 3 cut(s) 48, 63, 294
Msp20I TGGCCA 1 cut(s) 431
MspI CCGG 3 cut(s) 154, 352, 440
MunI CAATTG 1 cut(s) 377
MwoI GCNNNNNNNGC 1 cut(s) 163
NdeII GATC 1 cut(s) 99
NlaIII CATG 1 cut(s) 20
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 304
NspI RCATGY 1 cut(s) 20
PaeR7I CTCGAG 1 cut(s) 36
PciI ACATGT 1 cut(s) 16
PciSI GCTCTTC 1 cut(s) 329
PkrI GCNGC 2 cut(s) 278, 420
Ple19I CGATCG 1 cut(s) 102
PscI ACATGT 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 119
PvuI CGATCG 1 cut(s) 102
RsaI GTAC 2 cut(s) 75, 144
RsaNI GTAC 2 cut(s) 74, 143
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 3 cut(s) 48, 63, 294
SatI GCNGC 2 cut(s) 277, 419
Sau3AI GATC 1 cut(s) 99
Sau96I GGNCC 1 cut(s) 119
ScaI AGTACT 1 cut(s) 75
SduI GDGCHC 1 cut(s) 450
SetI ASST 7 cut(s) 141, 153, 189, 225, 278, 392, 447
Sfr274I CTCGAG 1 cut(s) 36
SlaI CTCGAG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
Sse9I AATT 3 cut(s) 80, 366, 377
SsiI CCGC 1 cut(s) 418
SspMI CTAG 3 cut(s) 77, 188, 309
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 2 cut(s) 318, 452
TaiI ACGT 1 cut(s) 153
TaqI TCGA 3 cut(s) 37, 92, 102
TasI AATT 3 cut(s) 80, 366, 377
TatI WGTACW 1 cut(s) 73
TauI GCSGC 1 cut(s) 421
Tru1I TTAA 3 cut(s) 48, 63, 294
Tru9I TTAA 3 cut(s) 48, 63, 294
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 1 cut(s) 276
Tsp45I GTSAC 1 cut(s) 304
VneI GTGCAC 1 cut(s) 446
XapI RAATTY 1 cut(s) 80
XceI RCATGY 1 cut(s) 20
XhoI CTCGAG 1 cut(s) 36
XmiI GTMKAC 1 cut(s) 161
XspI CTAG 3 cut(s) 77, 188, 309
ZrmI AGTACT 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.