Rh5BG007100

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017615
Physical Location & Seq
Forward (+)
499579 .. 500037
459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG007100.1

Sequence Viewer

Length: 459 bp
ATGGGAAACTGTATACGTGTTCCGTTCCATCCGCCCCTAGAGAAGATCAAAGTAGTAGTCTTTAATGGAGGAGTAGAAGAATTCAGTGCGTCGACAACAGCCGAAGAAATCACATCTGGCCCTTACACGGGTTACAAGCTGGTGCACAAGGCCAACCCATATACACCATTACCACCCAGTACACCACCCAGTACCAAGCTAGAACCTGGAGAGGTTTACCATCTTGTGCCAAACCTTGTATGGCTCAATGAGGCTTCGGTTCCTTCAAAAATGGCCCACCAAGAACAAGAAAGCGGCAAAAGACACAAGGTGAGGATTGTGGTGACCAGAGAGCAATTGGGGCTGTTGTTGAATGGCGCAAAGAAGATCCGGTCTTTAAACATTGGGAGTCGTGGGTCACAAGAGCCTCGCAAATGGAGGCCTTCTTTGGCTATGATTCCAGAGGTGCCAAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

152

Amino Acids

16.87

Weight (kDa)

9.68

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 148 1.5e-13 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017615)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00481
prunus_persica Prupe.4G005300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0000641
rosa_laevigata RLG00000008373 RLG00000030884
rosa_multiflora Rmu_sc0011992.1_g000013
rosa_roxburghii Rroxscaffold_1G00075560
rosa_samantha Rh5AG005500 Rh5BG007100 Rh5CG005800 Rh5DG005700
rosa_wichuraiana Rw5G000540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 445
AccI GTMKAC 2 cut(s) 13, 92
AciI CCGC 2 cut(s) 32, 294
AclWI GGATC 1 cut(s) 361
AcsI RAATTY 1 cut(s) 80
AdeI CACNNNGTG 1 cut(s) 310
AfaI GTAC 2 cut(s) 181, 193
AfiI CCNNNNNNNGG 2 cut(s) 127, 128
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 2 cut(s) 267, 352
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 2 cut(s) 139, 199
AluI AGCT 2 cut(s) 139, 199
Alw21I GWGCWC 1 cut(s) 147
Alw44I GTGCAC 1 cut(s) 143
AlwI GGATC 1 cut(s) 361
AoxI GGCC 4 cut(s) 118, 150, 273, 419
ApaLI GTGCAC 1 cut(s) 143
ApoI RAATTY 1 cut(s) 80
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
AspLEI GCGC 1 cut(s) 359
AspS9I GGNCC 2 cut(s) 119, 274
AsuHPI GGTGA 2 cut(s) 322, 334
BaeGI GKGCMC 1 cut(s) 147
BanI GGYRCC 1 cut(s) 445
Bbv12I GWGCWC 1 cut(s) 147
BccI CCATC 2 cut(s) 36, 228
BciT130I CCWGG 1 cut(s) 207
BfaI CTAG 2 cut(s) 38, 200
BisI GCNGC 1 cut(s) 295
BlsI GCNGC 1 cut(s) 296
Bme1390I CCNGG 1 cut(s) 207
BmgT120I GGNCC 2 cut(s) 119, 274
BmiI GGNNCC 2 cut(s) 261, 447
BmrFI CCNGG 1 cut(s) 207
BmrI ACTGGG 2 cut(s) 171, 183
BmuI ACTGGG 2 cut(s) 171, 183
BpmI CTGGAG 1 cut(s) 228
BsaAI YACGTR 1 cut(s) 17
BsaWI WCCGGW 1 cut(s) 369
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 128
Bse1I ACTGG 2 cut(s) 177, 189
BseBI CCWGG 1 cut(s) 207
BseGI GGATG 1 cut(s) 28
BseLI CCNNNNNNNGG 2 cut(s) 127, 128
BseNI ACTGG 2 cut(s) 177, 189
BseRI GAGGAG 1 cut(s) 84
BseSI GKGCMC 1 cut(s) 147
BshFI GGCC 4 cut(s) 120, 152, 275, 421
BshNI GGYRCC 1 cut(s) 445
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 1 cut(s) 370
BslI CCNNNNNNNGG 2 cut(s) 127, 128
BsnI GGCC 4 cut(s) 120, 152, 275, 421
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 2 cut(s) 45, 366
BspACI CCGC 2 cut(s) 32, 294
BspANI GGCC 4 cut(s) 120, 152, 275, 421
BspLI GGNNCC 2 cut(s) 261, 447
BspPI GGATC 1 cut(s) 361
BspT107I GGYRCC 1 cut(s) 445
BsrI ACTGG 2 cut(s) 177, 189
BssMI GATC 2 cut(s) 45, 366
BssNAI GTATAC 1 cut(s) 14
Bst1107I GTATAC 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 17
BstEII GGTNACC 1 cut(s) 322
BstF5I GGATG 1 cut(s) 28
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 2 cut(s) 48, 369
BstMBI GATC 2 cut(s) 45, 366
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstNI CCWGG 1 cut(s) 207
BstPI GGTNACC 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 205
BstSLI GKGCMC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 366
BstYI RGATCY 1 cut(s) 366
BstZ17I GTATAC 1 cut(s) 14
BsuRI GGCC 4 cut(s) 120, 152, 275, 421
BtsCI GGATG 1 cut(s) 28
BtsIMutI CAGTG 1 cut(s) 91
CfoI GCGC 1 cut(s) 359
Cfr13I GGNCC 2 cut(s) 119, 274
CseI GACGC 1 cut(s) 78
Csp6I GTAC 2 cut(s) 180, 192
CviQI GTAC 2 cut(s) 180, 192
DpnI GATC 2 cut(s) 47, 368
DpnII GATC 2 cut(s) 45, 366
DraI TTTAAA 1 cut(s) 378
DraIII CACNNNGTG 1 cut(s) 310
EciI GGCGGA 1 cut(s) 21
Eco147I AGGCCT 1 cut(s) 421
Eco91I GGTNACC 1 cut(s) 322
EcoO65I GGTNACC 1 cut(s) 322
EcoRI GAATTC 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 205
FaiI YATR 5 cut(s) 14, 160, 162, 241, 434
FblI GTMKAC 2 cut(s) 13, 92
Fnu4HI GCNGC 1 cut(s) 295
FokI GGATG 1 cut(s) 15
Fsp4HI GCNGC 1 cut(s) 295
FspBI CTAG 2 cut(s) 38, 200
GlaI GCGC 1 cut(s) 358
GluI GCNGC 1 cut(s) 295
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 4 cut(s) 120, 152, 275, 421
HapII CCGG 1 cut(s) 370
HgaI GACGC 1 cut(s) 78
HhaI GCGC 1 cut(s) 359
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HinfI GANTC 2 cut(s) 388, 436
HpaII CCGG 1 cut(s) 370
HphI GGTGA 2 cut(s) 322, 334
Hpy166II GTNNAC 5 cut(s) 14, 93, 145, 182, 217
Hpy188III TCNNGA 1 cut(s) 440
Hpy8I GTNNAC 5 cut(s) 14, 93, 145, 182, 217
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 2 cut(s) 273, 432
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 1 cut(s) 145
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpySE526I ACGT 1 cut(s) 16
HspAI GCGC 1 cut(s) 357
Kzo9I GATC 2 cut(s) 45, 366
LpnPI CCDG 9 cut(s) 102, 125, 190, 192, 202, 219, 340, 383, 453
MaeI CTAG 2 cut(s) 38, 200
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 3 cut(s) 131, 322, 396
MalI GATC 2 cut(s) 47, 368
MboI GATC 2 cut(s) 45, 366
MboII GAAGA 4 cut(s) 55, 89, 116, 376
MfeI CAATTG 1 cut(s) 335
MflI RGATCY 1 cut(s) 366
MhlI GDGCHC 1 cut(s) 147
MluCI AATT 2 cut(s) 80, 335
MlyI GAGTC 1 cut(s) 397
MnlI CCTC 7 cut(s) 62, 205, 244, 306, 411, 417, 436
MseI TTAA 2 cut(s) 63, 377
MspI CCGG 1 cut(s) 370
MspR9I CCNGG 1 cut(s) 207
MunI CAATTG 1 cut(s) 335
MvaI CCWGG 1 cut(s) 207
MwoI GCNNNNNNNGC 1 cut(s) 340
NdeII GATC 2 cut(s) 45, 366
NlaIV GGNNCC 2 cut(s) 261, 447
NmuCI GTSAC 2 cut(s) 322, 396
PceI AGGCCT 1 cut(s) 421
PfeI GAWTC 1 cut(s) 436
PkrI GCNGC 1 cut(s) 296
PleI GAGTC 1 cut(s) 396
PpsI GAGTC 1 cut(s) 396
Ppu21I YACGTR 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 205
PspEI GGTNACC 1 cut(s) 322
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 261, 447
PspPI GGNCC 2 cut(s) 119, 274
PsuI RGATCY 1 cut(s) 366
RsaI GTAC 2 cut(s) 181, 193
RsaNI GTAC 2 cut(s) 180, 192
SalI GTCGAC 1 cut(s) 91
SaqAI TTAA 2 cut(s) 63, 377
SatI GCNGC 1 cut(s) 295
Sau3AI GATC 2 cut(s) 45, 366
Sau96I GGNCC 2 cut(s) 119, 274
SchI GAGTC 1 cut(s) 397
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 1 cut(s) 147
SetI ASST 8 cut(s) 19, 141, 201, 208, 216, 237, 312, 447
Sse9I AATT 2 cut(s) 80, 335
SseBI AGGCCT 1 cut(s) 421
SsiI CCGC 2 cut(s) 32, 294
SspMI CTAG 2 cut(s) 38, 200
StuI AGGCCT 1 cut(s) 421
StyD4I CCNGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 1 cut(s) 19
TaqI TCGA 1 cut(s) 92
TasI AATT 2 cut(s) 80, 335
TatI WGTACW 1 cut(s) 179
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 2 cut(s) 63, 377
Tru9I TTAA 2 cut(s) 63, 377
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 2 cut(s) 322, 396
Tsp45I GTSAC 2 cut(s) 322, 396
TspGWI ACGGA 1 cut(s) 12
TspRI CASTG 1 cut(s) 91
VneI GTGCAC 1 cut(s) 143
XapI RAATTY 1 cut(s) 80
XcmI CCANNNNNNNNNTGG 2 cut(s) 237, 334
XmiI GTMKAC 2 cut(s) 13, 92
XspI CTAG 2 cut(s) 38, 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.