RLG00000008373

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0017615
Physical Location & Seq
Reverse (-)
29736322 .. 29737505
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008373

Sequence Viewer

Length: 615 bp
ATGGTGGTTGTAGTGACAACAGTTGAAGAAATCACATCTGGCGCTTACATGGGTTACAATCTGGTGCACAAGGCCAACCCATATACACCCTTGCCACCCAGCACCAAGCTTGAACCTGGAGAGGTTTACCATCTTGTGCCAAACCTTATATTGCTCAATAAGGCTTTGGTTCCTTCAAAAATAGCCCACCAAGAACAAAAAAGCGGCAAAAGACAGAAGGTGAGGATTGTGGTGACTATAGAGCAATTGGAGCTGCTGTTGAATGGCGCAAAGAAGATCCGATCTTTAAACATTGGGAGTCATGGGTCAGAGGAGCCTCGCAAATGGAGGCCCCATGGCCGCAAATCAGATCTCACCTTTTCTCTCAACCACCCAATCCTTTCCAAGCACTACCTGACTACAACCACATTGAACTCAGCTAGATCGATATGGCTACCTTGCAAAAATTCAAGCTCCTTACCACTCAATGCCCCGTCGTCACCTGAAGCCCCACGTGCAGCCCATCCGCCAGCCCCGTCATCCACCTCTTCCACCGTAAACCCTCAGATTGTTCCTCACCCGCCCCAACCGCCGCTGATTGCCTCGCTGAAGTACCTCGTCCGAGGCTCCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

205

Amino Acids

22.35

Weight (kDa)

10.0

Isoelectric Point (pI)

58.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017615)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00481
prunus_persica Prupe.4G005300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0000641
rosa_laevigata RLG00000008373 RLG00000030884
rosa_multiflora Rmu_sc0011992.1_g000013
rosa_roxburghii Rroxscaffold_1G00075560
rosa_samantha Rh5AG005500 Rh5BG007100 Rh5CG005800 Rh5DG005700
rosa_wichuraiana Rw5G000540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 204, 340, 506, 560, 569, 572
AclWI GGATC 1 cut(s) 271
AcoI YGGCCR 1 cut(s) 337
AcsI RAATTY 1 cut(s) 445
AcuI CTGAAG 2 cut(s) 504, 608
AcvI CACGTG 1 cut(s) 494
AfaI GTAC 1 cut(s) 593
AgsI TTSAA 6 cut(s) 26, 113, 177, 262, 412, 450
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 4 cut(s) 109, 253, 419, 453
AluI AGCT 4 cut(s) 109, 253, 419, 453
Alw21I GWGCWC 1 cut(s) 69
Alw44I GTGCAC 1 cut(s) 65
AlwI GGATC 1 cut(s) 271
AoxI GGCC 3 cut(s) 72, 329, 337
ApaLI GTGCAC 1 cut(s) 65
ApeKI GCWGC 2 cut(s) 253, 497
ApoI RAATTY 1 cut(s) 445
AspLEI GCGC 2 cut(s) 44, 269
AspS9I GGNCC 1 cut(s) 330
AsuHPI GGTGA 5 cut(s) 232, 244, 346, 471, 548
BaeGI GKGCMC 1 cut(s) 69
BbrPI CACGTG 1 cut(s) 494
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 2 cut(s) 240, 509
BccI CCATC 2 cut(s) 138, 510
BciT130I CCWGG 1 cut(s) 117
BfaI CTAG 1 cut(s) 420
BfmI CTRYAG 1 cut(s) 237
BfoI RGCGCY 1 cut(s) 45
BglII AGATCT 1 cut(s) 349
BisI GCNGC 5 cut(s) 205, 254, 340, 498, 572
BlsI GCNGC 5 cut(s) 206, 255, 341, 499, 573
Bme1390I CCNGG 1 cut(s) 117
BmgT120I GGNCC 1 cut(s) 330
BmiI GGNNCC 4 cut(s) 171, 315, 332, 607
BmrFI CCNGG 1 cut(s) 117
BpmI CTGGAG 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 425
BsaAI YACGTR 1 cut(s) 494
BsaJI CCNNGG 2 cut(s) 334, 601
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
BseBI CCWGG 1 cut(s) 117
BseCI ATCGAT 1 cut(s) 425
BseDI CCNNGG 2 cut(s) 334, 601
BseGI GGATG 2 cut(s) 502, 518
BseMII CTCAG 2 cut(s) 429, 557
BseRI GAGGAG 1 cut(s) 326
BseSI GKGCMC 1 cut(s) 69
BseXI GCAGC 2 cut(s) 240, 509
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 516
BshFI GGCC 3 cut(s) 74, 331, 339
BshVI ATCGAT 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 69
BsnI GGCC 3 cut(s) 74, 331, 339
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 4 cut(s) 276, 281, 349, 422
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 6 cut(s) 204, 340, 506, 560, 569, 572
BspANI GGCC 3 cut(s) 74, 331, 339
BspCNI CTCAG 2 cut(s) 428, 556
BspDI ATCGAT 1 cut(s) 425
BspLI GGNNCC 4 cut(s) 171, 315, 332, 607
BspPI GGATC 1 cut(s) 271
BssECI CCNNGG 2 cut(s) 334, 601
BssMI GATC 4 cut(s) 276, 281, 349, 422
BssT1I CCWWGG 1 cut(s) 334
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 22, 535
Bst6I CTCTTC 1 cut(s) 532
BstBAI YACGTR 1 cut(s) 494
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 2 cut(s) 415, 543
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 2 cut(s) 502, 518
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 2 cut(s) 44, 269
BstKTI GATC 4 cut(s) 279, 284, 352, 425
BstMBI GATC 4 cut(s) 276, 281, 349, 422
BstMWI GCNNNNNNNGC 3 cut(s) 250, 494, 568
BstNI CCWGG 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 237
BstSLI GKGCMC 1 cut(s) 69
BstV1I GCAGC 2 cut(s) 240, 509
BstX2I RGATCY 2 cut(s) 276, 349
BstYI RGATCY 2 cut(s) 276, 349
Bsu15I ATCGAT 1 cut(s) 425
BsuRI GGCC 3 cut(s) 74, 331, 339
BsuTUI ATCGAT 1 cut(s) 425
BtgI CCRYGG 1 cut(s) 334
BtsCI GGATG 2 cut(s) 502, 518
Cac8I GCNNGC 1 cut(s) 510
CfoI GCGC 2 cut(s) 44, 269
Cfr13I GGNCC 1 cut(s) 330
ClaI ATCGAT 1 cut(s) 425
Csp6I GTAC 1 cut(s) 592
CviAII CATG 3 cut(s) 49, 302, 335
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 2 cut(s) 415, 543
DpnI GATC 4 cut(s) 278, 283, 351, 424
DpnII GATC 4 cut(s) 276, 281, 349, 422
DraI TTTAAA 1 cut(s) 288
EaeI YGGCCR 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 532
EarI CTCTTC 1 cut(s) 532
EciI GGCGGA 1 cut(s) 495
Eco130I CCWWGG 1 cut(s) 334
Eco57I CTGAAG 2 cut(s) 504, 608
Eco72I CACGTG 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 334
FaeI CATG 3 cut(s) 52, 305, 338
FaiI YATR 8 cut(s) 50, 82, 84, 149, 239, 303, 336, 430
FatI CATG 3 cut(s) 48, 301, 334
FauI CCCGC 1 cut(s) 567
Fnu4HI GCNGC 5 cut(s) 205, 254, 340, 498, 572
FokI GGATG 2 cut(s) 489, 505
Fsp4HI GCNGC 5 cut(s) 205, 254, 340, 498, 572
FspBI CTAG 1 cut(s) 420
GlaI GCGC 2 cut(s) 43, 268
GluI GCNGC 5 cut(s) 205, 254, 340, 498, 572
GsaI CCCAGC 1 cut(s) 102
GsuI CTGGAG 1 cut(s) 138
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 3 cut(s) 74, 331, 339
HhaI GCGC 2 cut(s) 44, 269
Hin1II CATG 3 cut(s) 52, 305, 338
Hin6I GCGC 2 cut(s) 42, 267
HinP1I GCGC 2 cut(s) 42, 267
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 1 cut(s) 298
HphI GGTGA 5 cut(s) 232, 244, 346, 471, 548
Hpy166II GTNNAC 3 cut(s) 67, 127, 538
Hpy188I TCNGA 5 cut(s) 281, 310, 349, 546, 602
Hpy8I GTNNAC 3 cut(s) 67, 127, 538
Hpy99I CGWCG 1 cut(s) 478
HpyAV CCTTC 2 cut(s) 183, 211
HpyCH4III ACNGT 2 cut(s) 22, 535
HpyCH4IV ACGT 1 cut(s) 493
HpyCH4V TGCA 3 cut(s) 67, 441, 497
HpyF10VI GCNNNNNNNGC 3 cut(s) 250, 494, 568
HpyF3I CTNAG 2 cut(s) 415, 543
HpySE526I ACGT 1 cut(s) 493
Hsp92II CATG 3 cut(s) 52, 305, 338
HspAI GCGC 2 cut(s) 42, 267
Kzo9I GATC 4 cut(s) 276, 281, 349, 422
LmnI GCTCC 4 cut(s) 250, 313, 458, 611
LpnPI CCDG 8 cut(s) 24, 47, 102, 112, 129, 407, 495, 522
Lsp1109I GCAGC 2 cut(s) 240, 509
MaeI CTAG 1 cut(s) 420
MaeII ACGT 1 cut(s) 493
MaeIII GTNAC 4 cut(s) 13, 53, 232, 477
MalI GATC 4 cut(s) 278, 283, 351, 424
MboI GATC 4 cut(s) 276, 281, 349, 422
MboII GAAGA 3 cut(s) 38, 286, 519
MfeI CAATTG 1 cut(s) 245
MflI RGATCY 2 cut(s) 276, 349
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 2 cut(s) 245, 445
MlyI GAGTC 1 cut(s) 307
MseI TTAA 1 cut(s) 287
MspA1I CMGCKG 1 cut(s) 574
MspR9I CCNGG 1 cut(s) 117
MunI CAATTG 1 cut(s) 245
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 3 cut(s) 250, 494, 568
NcoI CCATGG 1 cut(s) 334
NdeII GATC 4 cut(s) 276, 281, 349, 422
NlaIII CATG 3 cut(s) 52, 305, 338
NlaIV GGNNCC 4 cut(s) 171, 315, 332, 607
NmuCI GTSAC 3 cut(s) 13, 232, 477
PkrI GCNGC 5 cut(s) 206, 255, 341, 499, 573
PleI GAGTC 1 cut(s) 306
PmaCI CACGTG 1 cut(s) 494
PmlI CACGTG 1 cut(s) 494
PpsI GAGTC 1 cut(s) 306
Ppu21I YACGTR 1 cut(s) 494
Psp6I CCWGG 1 cut(s) 115
PspCI CACGTG 1 cut(s) 494
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 4 cut(s) 171, 315, 332, 607
PspPI GGNCC 1 cut(s) 330
PsuI RGATCY 2 cut(s) 276, 349
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 5 cut(s) 205, 254, 340, 498, 572
Sau3AI GATC 4 cut(s) 276, 281, 349, 422
Sau96I GGNCC 1 cut(s) 330
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 1 cut(s) 69
SfcI CTRYAG 1 cut(s) 237
Sse9I AATT 2 cut(s) 245, 445
SsiI CCGC 6 cut(s) 204, 340, 506, 560, 569, 572
SspMI CTAG 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 2 cut(s) 22, 535
TaiI ACGT 1 cut(s) 496
TaqI TCGA 1 cut(s) 425
TasI AATT 2 cut(s) 245, 445
TauI GCSGC 3 cut(s) 207, 342, 574
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TseFI GTSAC 3 cut(s) 13, 232, 477
TseI GCWGC 2 cut(s) 253, 497
Tsp45I GTSAC 3 cut(s) 13, 232, 477
VneI GTGCAC 1 cut(s) 65
XapI RAATTY 1 cut(s) 445
XspI CTAG 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.