FvH4_3g06160

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0017618
Physical Location & Seq
Reverse (-)
3541951 .. 3543045
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06160.t1

Sequence Viewer

Length: 1095 bp
ATGTTTGTCATACCAGTTCAAGAAGCTGAACCAGCAATTTCTCAGCCCAGAAATTTCAAGTGGTGGCTCCGCATCGCCATCTACACACTCTTTGTTATGCTTGGCCAGTCAGCAGCTATACTCTTGGGACGACTGTACTACAACAAAGGTGGGAAAAGCATTTGGATGGCAACCCTAGTGCAATTTATAGGGTTCCCAATCCTAATTCCTTACCACTGTCTCTCATCATCCAAAAAGATCACTACAGAAAGCAATGTGAACCTGCTATCTTCATCAGTTCTTGCATTGATCTATCTCTCTTTTGGTGTACTACTAGCAGCAGATAGCATGCTGTATTCCATTGGACTATTGCACCTTCCAGTTTCAACATTTTCACTCATTTGTGCATCTCAGTTGGTCTTCAATGCTTTCTTTTCCTTCTTCCTCAATGCACAAAAGTTCACACCTTACATTGTCAATTCCCTAGTCCTTCTCACCATCTCCTCCATGTTACTTGTGTTTCACGCCACCTCCATGAATGTAGCTGGAATTTCCAAAGAGATGTACATAATGGGAATCGTGTGCACTGTTGGTGCATCTGCTGGCTATGGTTTGATGTTTTCCCTTACACAGGTAGCCTTCCAAAAAGTTCTAAAGTCTAATACATTTACTGCTGTGTTAGAAATGATAATCTTCGAATCTGTAGTTGCAACCTGTGCTACCCTAGTTGGACTTTTCGTCAGCAACGAGAAGAAGGGTTTAAAGAGAGAAATGGAGGAGTTTGAGCTTGGGAGGCTGTTCTACTTTGTGACTTTGGTTTGCACAGCTGCAGCATGGCAAGCTTTCAATATTGGCGCAATAGGGCTGATTTTTGAGGTTTCGGCCTTGTTTTCAAATGTTACATGTGTTTCGGGCTTGCCATTTGTTCCAGTTCTCGCAGTTATATTCTTCAATGATAGTATGGATGGAATAAAGGTTATTGCAACAGTGTTGGCAATCTGGGGATTTGCTTCATATCTCTATCAGCACTATCTTGACAATTCGGACTCCAACCCAGAAATTAAACCTGTAACAGAAATTTCAATGATTCCTTTGCTGAAAGAGACCAACTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

365

Amino Acids

40.13

Weight (kDa)

6.88

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 24 - 341 2.5e-114 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g06160
malus_domestica MD05G1289900.v1.1
prunus_persica Prupe.4G075800_v2.0.a1
rosa_laevigata RLG00000031994
rosa_multiflora Rmu_sc0004521.1_g000005
rosa_roxburghii Rroxscaffold_1G00063410
rosa_rugosa Rorug05G0007800
rosa_samantha Rh5AG102100 Rh5BG098400 Rh5CG110600 Rh5DG097700
rosa_wichuraiana Rw5G008910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 270
AciI CCGC 1 cut(s) 70
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 3 cut(s) 52, 528, 1056
AfaI GTAC 3 cut(s) 137, 309, 545
AfiI CCNNNNNNNGG 1 cut(s) 610
AflIII ACRYGT 1 cut(s) 881
AgsI TTSAA 8 cut(s) 20, 58, 366, 403, 826, 873, 931, 1062
AhdI GACNNNNNGTC 1 cut(s) 716
AluBI AGCT 6 cut(s) 26, 116, 524, 766, 806, 821
AluI AGCT 6 cut(s) 26, 116, 524, 766, 806, 821
Alw21I GWGCWC 1 cut(s) 566
Alw26I GTCTC 2 cut(s) 224, 1076
Alw44I GTGCAC 1 cut(s) 562
AoxI GGCC 2 cut(s) 103, 861
ApaLI GTGCAC 1 cut(s) 562
ApeKI GCWGC 4 cut(s) 113, 317, 806, 809
ApoI RAATTY 3 cut(s) 52, 528, 1056
AspLEI GCGC 1 cut(s) 836
AsuHPI GGTGA 1 cut(s) 466
AsuII TTCGAA 1 cut(s) 675
BaeGI GKGCMC 1 cut(s) 566
BalI TGGCCA 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 391
Bbv12I GWGCWC 1 cut(s) 566
BbvI GCAGC 4 cut(s) 125, 329, 793, 821
BccI CCATC 4 cut(s) 86, 160, 485, 938
BcoDI GTCTC 2 cut(s) 224, 1076
BfaI CTAG 4 cut(s) 176, 314, 464, 704
BfmI CTRYAG 3 cut(s) 243, 681, 807
BfuAI ACCTGC 1 cut(s) 270
BisI GCNGC 4 cut(s) 114, 318, 807, 810
BlsI GCNGC 4 cut(s) 115, 319, 808, 811
BmeRI GACNNNNNGTC 1 cut(s) 716
BmiI GGNNCC 2 cut(s) 68, 194
BmsI GCATC 3 cut(s) 81, 395, 584
BpiI GAAGAC 1 cut(s) 391
Bpu14I TTCGAA 1 cut(s) 675
BsaI GGTCTC 1 cut(s) 1076
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 1 cut(s) 610
Bse1I ACTGG 4 cut(s) 14, 106, 359, 908
Bse3DI GCAATG 1 cut(s) 259
BseGI GGATG 3 cut(s) 171, 227, 949
BseLI CCNNNNNNNGG 1 cut(s) 610
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 2 cut(s) 56, 404
BseNI ACTGG 4 cut(s) 14, 106, 359, 908
BseRI GAGGAG 2 cut(s) 472, 770
BseSI GKGCMC 1 cut(s) 566
BseXI GCAGC 4 cut(s) 125, 329, 793, 821
BshFI GGCC 2 cut(s) 105, 863
BsiHKAI GWGCWC 1 cut(s) 566
BslFI GGGAC 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 610
BsmAI GTCTC 2 cut(s) 224, 1076
BsmFI GGGAC 1 cut(s) 141
BsnI GGCC 2 cut(s) 105, 863
Bso31I GGTCTC 1 cut(s) 1076
Bsp119I TTCGAA 1 cut(s) 675
Bsp1286I GDGCHC 1 cut(s) 566
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 2 cut(s) 237, 288
BspACI CCGC 1 cut(s) 70
BspANI GGCC 2 cut(s) 105, 863
BspCNI CTCAG 2 cut(s) 55, 403
BspLI GGNNCC 2 cut(s) 68, 194
BspMAI CTGCAG 1 cut(s) 811
BspMI ACCTGC 1 cut(s) 270
BspT104I TTCGAA 1 cut(s) 675
BspTNI GGTCTC 1 cut(s) 1076
BsrDI GCAATG 1 cut(s) 259
BsrGI TGTACA 1 cut(s) 543
BsrI ACTGG 4 cut(s) 14, 106, 359, 908
BssMI GATC 2 cut(s) 237, 288
Bst4CI ACNGT 4 cut(s) 135, 218, 568, 967
BstAPI GCANNNNNTGC 1 cut(s) 695
BstAUI TGTACA 1 cut(s) 543
BstBI TTCGAA 1 cut(s) 675
BstC8I GCNNGC 4 cut(s) 329, 583, 819, 896
BstDEI CTNAG 2 cut(s) 42, 390
BstENI CCTNNNNNAGG 1 cut(s) 608
BstF5I GGATG 3 cut(s) 171, 227, 949
BstHHI GCGC 1 cut(s) 836
BstKTI GATC 2 cut(s) 240, 291
BstMAI GTCTC 2 cut(s) 224, 1076
BstMBI GATC 2 cut(s) 237, 288
BstMWI GCNNNNNNNGC 4 cut(s) 32, 695, 772, 818
BstNSI RCATGY 2 cut(s) 331, 885
BstSFI CTRYAG 3 cut(s) 243, 681, 807
BstSLI GKGCMC 1 cut(s) 566
BstV1I GCAGC 4 cut(s) 125, 329, 793, 821
BstV2I GAAGAC 1 cut(s) 391
BsuRI GGCC 2 cut(s) 105, 863
BtgZI GCGATG 1 cut(s) 58
BtsCI GGATG 3 cut(s) 171, 227, 949
BtsIMutI CAGTG 3 cut(s) 214, 564, 972
BveI ACCTGC 1 cut(s) 270
Cac8I GCNNGC 4 cut(s) 329, 583, 819, 896
CfoI GCGC 1 cut(s) 836
Csp6I GTAC 3 cut(s) 136, 308, 544
CspCI CAANNNNNGTGG 2 cut(s) 130, 165
CviAII CATG 5 cut(s) 328, 487, 514, 813, 882
CviQI GTAC 3 cut(s) 136, 308, 544
DdeI CTNAG 2 cut(s) 42, 390
DpnI GATC 2 cut(s) 239, 290
DpnII GATC 2 cut(s) 237, 288
DraI TTTAAA 1 cut(s) 741
DriI GACNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 1 cut(s) 103
Eam1105I GACNNNNNGTC 1 cut(s) 716
Eco31I GGTCTC 1 cut(s) 1076
EcoNI CCTNNNNNAGG 1 cut(s) 608
FaeI CATG 5 cut(s) 331, 490, 517, 816, 885
FaqI GGGAC 1 cut(s) 141
FatI CATG 5 cut(s) 327, 486, 513, 812, 881
Fnu4HI GCNGC 4 cut(s) 114, 318, 807, 810
FokI GGATG 3 cut(s) 178, 214, 956
Fsp4HI GCNGC 4 cut(s) 114, 318, 807, 810
FspBI CTAG 4 cut(s) 176, 314, 464, 704
GlaI GCGC 1 cut(s) 835
GluI GCNGC 4 cut(s) 114, 318, 807, 810
HaeIII GGCC 2 cut(s) 105, 863
HhaI GCGC 1 cut(s) 836
Hin1II CATG 5 cut(s) 331, 490, 517, 816, 885
Hin6I GCGC 1 cut(s) 834
HinP1I GCGC 1 cut(s) 834
HindIII AAGCTT 1 cut(s) 819
HinfI GANTC 4 cut(s) 555, 677, 1025, 1066
HphI GGTGA 1 cut(s) 466
Hpy166II GTNNAC 4 cut(s) 259, 308, 441, 564
Hpy188I TCNGA 1 cut(s) 1024
Hpy188III TCNNGA 2 cut(s) 20, 1013
Hpy8I GTNNAC 4 cut(s) 259, 308, 441, 564
HpyAV CCTTC 5 cut(s) 365, 427, 479, 628, 727
HpyCH4III ACNGT 4 cut(s) 135, 218, 568, 967
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 695, 772, 818
HpyF3I CTNAG 2 cut(s) 42, 390
Hsp92II CATG 5 cut(s) 331, 490, 517, 816, 885
HspAI GCGC 1 cut(s) 834
Kzo9I GATC 2 cut(s) 237, 288
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 4 cut(s) 125, 329, 793, 821
LweI GCATC 3 cut(s) 81, 395, 584
MaeI CTAG 4 cut(s) 176, 314, 464, 704
MaeIII GTNAC 4 cut(s) 489, 787, 877, 1048
MalI GATC 2 cut(s) 239, 290
MboI GATC 2 cut(s) 237, 288
MboII GAAGA 6 cut(s) 261, 391, 412, 664, 742, 919
MhlI GDGCHC 1 cut(s) 566
MlsI TGGCCA 1 cut(s) 105
MluCI AATT 9 cut(s) 36, 52, 182, 204, 457, 528, 1018, 1038, 1056
MluNI TGGCCA 1 cut(s) 105
MlyI GAGTC 1 cut(s) 1019
MmeI TCCRAC 2 cut(s) 688, 1053
MnlI CCTC 6 cut(s) 434, 493, 520, 748, 765, 847
Mox20I TGGCCA 1 cut(s) 105
MscI TGGCCA 1 cut(s) 105
MseI TTAA 2 cut(s) 740, 1041
MslI CAYNNNNRTG 2 cut(s) 164, 512
Msp20I TGGCCA 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 806
MwoI GCNNNNNNNGC 4 cut(s) 32, 695, 772, 818
NdeII GATC 2 cut(s) 237, 288
NlaIII CATG 5 cut(s) 331, 490, 517, 816, 885
NlaIV GGNNCC 2 cut(s) 68, 194
NmuCI GTSAC 1 cut(s) 787
NspI RCATGY 2 cut(s) 331, 885
NspV TTCGAA 1 cut(s) 675
PaeI GCATGC 1 cut(s) 331
PciI ACATGT 1 cut(s) 881
PcsI WCGNNNNNNNCGW 1 cut(s) 723
PfeI GAWTC 3 cut(s) 555, 677, 1066
PkrI GCNGC 4 cut(s) 115, 319, 808, 811
PleI GAGTC 1 cut(s) 1019
PpsI GAGTC 1 cut(s) 1019
PscI ACATGT 1 cut(s) 881
PspN4I GGNNCC 2 cut(s) 68, 194
PstI CTGCAG 1 cut(s) 811
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 3 cut(s) 137, 309, 545
RsaNI GTAC 3 cut(s) 136, 308, 544
RseI CAYNNNNRTG 2 cut(s) 164, 512
SaqAI TTAA 2 cut(s) 740, 1041
SatI GCNGC 4 cut(s) 114, 318, 807, 810
Sau3AI GATC 2 cut(s) 237, 288
SchI GAGTC 1 cut(s) 1019
SduI GDGCHC 1 cut(s) 566
SfaNI GCATC 3 cut(s) 81, 395, 584
SfcI CTRYAG 3 cut(s) 243, 681, 807
SfuI TTCGAA 1 cut(s) 675
SmiMI CAYNNNNRTG 2 cut(s) 164, 512
SphI GCATGC 1 cut(s) 331
Sse9I AATT 9 cut(s) 36, 52, 182, 204, 457, 528, 1018, 1038, 1056
SsiI CCGC 1 cut(s) 70
SspI AATATT 1 cut(s) 829
SspMI CTAG 4 cut(s) 176, 314, 464, 704
TaaI ACNGT 4 cut(s) 135, 218, 568, 967
TaqI TCGA 1 cut(s) 675
TasI AATT 9 cut(s) 36, 52, 182, 204, 457, 528, 1018, 1038, 1056
TatI WGTACW 3 cut(s) 135, 307, 543
TfiI GAWTC 3 cut(s) 555, 677, 1066
Tru1I TTAA 2 cut(s) 740, 1041
Tru9I TTAA 2 cut(s) 740, 1041
TscAI CASTG 3 cut(s) 221, 571, 972
TseFI GTSAC 1 cut(s) 787
TseI GCWGC 4 cut(s) 113, 317, 806, 809
Tsp45I GTSAC 1 cut(s) 787
TspDTI ATGAA 3 cut(s) 261, 530, 981
TspRI CASTG 3 cut(s) 221, 571, 972
VneI GTGCAC 1 cut(s) 562
XagI CCTNNNNNAGG 1 cut(s) 608
XapI RAATTY 3 cut(s) 52, 528, 1056
XceI RCATGY 2 cut(s) 331, 885
XspI CTAG 4 cut(s) 176, 314, 464, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.