Rw5G008910

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0017618
Physical Location & Seq
Forward (+)
9593927 .. 9597484
3558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G008910.1

Sequence Viewer

Length: 1071 bp
ATGTACATGCCAAAATTCCATCCCAGAAATTTTAAGTGGTGGCTCCGTATCGGCGTCTACACACTCTTTGTTCTCTTTGGCCAGTCAGCAGCTATACTCTTGGGACGACTGTACTACAACAAAGGTGGAAAAAGCATATGGATGGCAACCCTAGTGCAATTTGTAGGGTTTCCAATCCTAATTCCTTACCAATGTCTCTCATCATCCAAAAAAATCACTACAGAAAGCAATGTGAACCTGCCATCTTCATCAGTTCTTGCATTGATCTATCTCTCTTTTGGTGTGCTACTAGCATCAGATAGCATGCTGTATTCCATTGGACTCTTGTACCTTCCAGTTTCGACATTTTCACTCATTTGTGCATCTCAGTTGGTCTTCAATGCTTTCTTCTCCTTCTTCCTCAATTCACAAAAGTTCACACCTTACATTGTCAATTCCCTAGTCCTTCTCACCATCTCCTCCATGTTACTTGTGTTTCATGCCAACTCCATAAATGTCGCTGGAGTTTCTAAAGAGAAGTACATAATCGGAATCACGTGCACCATTGGTGCATCTGCTGGCTATGGTTTGATGTTTTCCCTTACGCAGGTAGCTTTCCAGAAAGTTCTAAAATCAAATGCATTTAGTGCTGTGTTAGAAATGATAATCTTTGAATCTATAGTTGCCACCTGTGCTACCCTAGTTGGACTTTTCGTCAGCAAAGAGATGAAGGGTTTAAAGAGAGAGATTGAGGAGTTTGAGCTGGGGAGGCTATTCTACTTGGTGACTTTGGTTTGCACAGCTGCAGCATGGCAAGCTTTCAATATTGGTGCAATAGGACTGGTTTTTGAGGTTTCTTCCTTATTCTCGAATGTTATATGTGTTTCGGGCTTGCCATTTGTTCCAGTTCTCGCAGTGATATTCTTCAATGATAGTCTGGATGGAATAAAGGTTATTGCAACAGTGTTGGCAATCTGGGGATTTGCTTCATATCTCTATCAGCATTATCTCGACAATTCGAACTCCAACCCTGAAACCAAACCTGTAACAGAAATTTCAATGATTCCTTTACTGAAAGAGACCAACTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

356

Amino Acids

39.33

Weight (kDa)

8.72

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 16 - 333 7.9e-114 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g06160
malus_domestica MD05G1289900.v1.1
prunus_persica Prupe.4G075800_v2.0.a1
rosa_laevigata RLG00000031994
rosa_multiflora Rmu_sc0004521.1_g000005
rosa_roxburghii Rroxscaffold_1G00063410
rosa_rugosa Rorug05G0007800
rosa_samantha Rh5AG102100 Rh5BG098400 Rh5CG110600 Rh5DG097700
rosa_wichuraiana Rw5G008910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 246, 577
AccI GTMKAC 1 cut(s) 57
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 3 cut(s) 14, 28, 1032
AcvI CACGTG 1 cut(s) 537
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 4 cut(s) 5, 113, 329, 521
AfiI CCNNNNNNNGG 1 cut(s) 586
AgsI TTSAA 5 cut(s) 379, 653, 802, 907, 1038
AhdI GACNNNNNGTC 1 cut(s) 692
AluBI AGCT 5 cut(s) 92, 593, 742, 782, 797
AluI AGCT 5 cut(s) 92, 593, 742, 782, 797
Alw21I GWGCWC 1 cut(s) 542
Alw26I GTCTC 2 cut(s) 200, 1052
Alw44I GTGCAC 1 cut(s) 538
AoxI GGCC 1 cut(s) 79
ApaLI GTGCAC 1 cut(s) 538
ApeKI GCWGC 3 cut(s) 89, 782, 785
ApoI RAATTY 3 cut(s) 14, 28, 1032
AsuHPI GGTGA 2 cut(s) 442, 775
AsuII TTCGAA 1 cut(s) 998
BaeGI GKGCMC 1 cut(s) 542
BaeI ACNNNNGTAYC 2 cut(s) 311, 344
BalI TGGCCA 1 cut(s) 81
BbrPI CACGTG 1 cut(s) 537
BbsI GAAGAC 1 cut(s) 367
Bbv12I GWGCWC 1 cut(s) 542
BbvI GCAGC 3 cut(s) 101, 769, 797
BccI CCATC 5 cut(s) 27, 136, 250, 461, 914
BcoDI GTCTC 2 cut(s) 200, 1052
BfaI CTAG 4 cut(s) 152, 290, 440, 680
BfmI CTRYAG 3 cut(s) 219, 657, 783
BfuAI ACCTGC 2 cut(s) 246, 577
BisI GCNGC 3 cut(s) 90, 783, 786
BlsI GCNGC 3 cut(s) 91, 784, 787
BmeRI GACNNNNNGTC 1 cut(s) 692
BmiI GGNNCC 1 cut(s) 44
BmsI GCATC 3 cut(s) 302, 371, 560
BpiI GAAGAC 1 cut(s) 367
BpmI CTGGAG 1 cut(s) 522
Bpu14I TTCGAA 1 cut(s) 998
BsaAI YACGTR 1 cut(s) 537
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 1052
Bsc4I CCNNNNNNNGG 1 cut(s) 586
Bse1I ACTGG 4 cut(s) 82, 335, 825, 884
Bse3DI GCAATG 1 cut(s) 235
BseGI GGATG 4 cut(s) 19, 147, 203, 925
BseLI CCNNNNNNNGG 1 cut(s) 586
BseMI GCAATG 1 cut(s) 235
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 4 cut(s) 82, 335, 825, 884
BseRI GAGGAG 2 cut(s) 448, 746
BseSI GKGCMC 1 cut(s) 542
BseXI GCAGC 3 cut(s) 101, 769, 797
BseYI CCCAGC 1 cut(s) 742
BshFI GGCC 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 542
BslFI GGGAC 1 cut(s) 117
BslI CCNNNNNNNGG 1 cut(s) 586
BsmAI GTCTC 2 cut(s) 200, 1052
BsmFI GGGAC 1 cut(s) 117
BsnI GGCC 1 cut(s) 81
Bso31I GGTCTC 1 cut(s) 1052
Bsp119I TTCGAA 1 cut(s) 998
Bsp1286I GDGCHC 1 cut(s) 542
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 264
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 1 cut(s) 44
BspMAI CTGCAG 1 cut(s) 787
BspMI ACCTGC 2 cut(s) 246, 577
BspT104I TTCGAA 1 cut(s) 998
BspTNI GGTCTC 1 cut(s) 1052
BsrDI GCAATG 1 cut(s) 235
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 4 cut(s) 82, 335, 825, 884
BssMI GATC 1 cut(s) 264
BssNI GRCGYC 1 cut(s) 54
Bst4CI ACNGT 2 cut(s) 111, 943
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 626
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 537
BstBI TTCGAA 1 cut(s) 998
BstC8I GCNNGC 4 cut(s) 305, 559, 795, 872
BstDEI CTNAG 1 cut(s) 366
BstENI CCTNNNNNAGG 1 cut(s) 584
BstF5I GGATG 4 cut(s) 19, 147, 203, 925
BstKTI GATC 1 cut(s) 267
BstMAI GTCTC 2 cut(s) 200, 1052
BstMBI GATC 1 cut(s) 264
BstMWI GCNNNNNNNGC 4 cut(s) 626, 671, 748, 794
BstNSI RCATGY 2 cut(s) 10, 307
BstSFI CTRYAG 3 cut(s) 219, 657, 783
BstSLI GKGCMC 1 cut(s) 542
BstV1I GCAGC 3 cut(s) 101, 769, 797
BstV2I GAAGAC 1 cut(s) 367
BsuRI GGCC 1 cut(s) 81
BtsCI GGATG 4 cut(s) 19, 147, 203, 925
BtsI GCAGTG 1 cut(s) 900
BtsIMutI CAGTG 2 cut(s) 900, 948
BveI ACCTGC 2 cut(s) 246, 577
Cac8I GCNNGC 4 cut(s) 305, 559, 795, 872
CseI GACGC 1 cut(s) 43
Csp6I GTAC 4 cut(s) 4, 112, 328, 520
CspCI CAANNNNNGTGG 2 cut(s) 106, 141
CviAII CATG 5 cut(s) 7, 304, 463, 479, 789
CviQI GTAC 4 cut(s) 4, 112, 328, 520
DdeI CTNAG 1 cut(s) 366
DpnI GATC 1 cut(s) 266
DpnII GATC 1 cut(s) 264
DraI TTTAAA 1 cut(s) 717
DriI GACNNNNNGTC 1 cut(s) 692
EaeI YGGCCR 1 cut(s) 79
Eam1105I GACNNNNNGTC 1 cut(s) 692
Eco31I GGTCTC 1 cut(s) 1052
Eco72I CACGTG 1 cut(s) 537
EcoNI CCTNNNNNAGG 1 cut(s) 584
EcoT22I ATGCAT 1 cut(s) 622
FaeI CATG 5 cut(s) 10, 307, 466, 482, 792
FaqI GGGAC 1 cut(s) 117
FatI CATG 5 cut(s) 6, 303, 462, 478, 788
FauNDI CATATG 1 cut(s) 137
FblI GTMKAC 1 cut(s) 57
Fnu4HI GCNGC 3 cut(s) 90, 783, 786
FokI GGATG 4 cut(s) 6, 154, 190, 932
Fsp4HI GCNGC 3 cut(s) 90, 783, 786
FspBI CTAG 4 cut(s) 152, 290, 440, 680
GluI GCNGC 3 cut(s) 90, 783, 786
GsaI CCCAGC 1 cut(s) 746
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 1 cut(s) 81
HgaI GACGC 1 cut(s) 43
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 5 cut(s) 10, 307, 466, 482, 792
HindIII AAGCTT 1 cut(s) 795
HinfI GANTC 4 cut(s) 321, 531, 653, 1042
HphI GGTGA 2 cut(s) 442, 775
Hpy166II GTNNAC 4 cut(s) 58, 235, 417, 540
Hpy188I TCNGA 2 cut(s) 298, 530
Hpy188III TCNNGA 4 cut(s) 598, 847, 917, 989
Hpy8I GTNNAC 4 cut(s) 58, 235, 417, 540
HpyAV CCTTC 4 cut(s) 341, 403, 455, 703
HpyCH4III ACNGT 2 cut(s) 111, 943
HpyCH4IV ACGT 1 cut(s) 536
HpyF10VI GCNNNNNNNGC 4 cut(s) 626, 671, 748, 794
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 1 cut(s) 536
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 5 cut(s) 10, 307, 466, 482, 792
Kzo9I GATC 1 cut(s) 264
LmnI GCTCC 1 cut(s) 48
Lsp1109I GCAGC 3 cut(s) 101, 769, 797
LweI GCATC 3 cut(s) 302, 371, 560
MaeI CTAG 4 cut(s) 152, 290, 440, 680
MaeII ACGT 1 cut(s) 536
MaeIII GTNAC 3 cut(s) 465, 763, 1024
MalI GATC 1 cut(s) 266
MboI GATC 1 cut(s) 264
MboII GAAGA 6 cut(s) 237, 367, 379, 388, 828, 895
MhlI GDGCHC 1 cut(s) 542
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 8 cut(s) 14, 28, 158, 180, 403, 433, 994, 1032
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 1 cut(s) 315
MmeI TCCRAC 2 cut(s) 664, 1029
MnlI CCTC 5 cut(s) 410, 469, 724, 741, 823
Mox20I TGGCCA 1 cut(s) 81
Mph1103I ATGCAT 1 cut(s) 622
MscI TGGCCA 1 cut(s) 81
MseI TTAA 2 cut(s) 33, 716
MslI CAYNNNNRTG 1 cut(s) 140
Msp20I TGGCCA 1 cut(s) 81
MspA1I CMGCKG 1 cut(s) 782
MwoI GCNNNNNNNGC 4 cut(s) 626, 671, 748, 794
NdeI CATATG 1 cut(s) 137
NdeII GATC 1 cut(s) 264
NlaIII CATG 5 cut(s) 10, 307, 466, 482, 792
NlaIV GGNNCC 1 cut(s) 44
NmuCI GTSAC 1 cut(s) 763
NsiI ATGCAT 1 cut(s) 622
NspI RCATGY 2 cut(s) 10, 307
NspV TTCGAA 1 cut(s) 998
PaeI GCATGC 1 cut(s) 307
PfeI GAWTC 3 cut(s) 531, 653, 1042
PkrI GCNGC 3 cut(s) 91, 784, 787
PleI GAGTC 1 cut(s) 315
PmaCI CACGTG 1 cut(s) 537
PmlI CACGTG 1 cut(s) 537
PpsI GAGTC 1 cut(s) 315
Ppu21I YACGTR 1 cut(s) 537
PspCI CACGTG 1 cut(s) 537
PspFI CCCAGC 1 cut(s) 742
PspN4I GGNNCC 1 cut(s) 44
PstI CTGCAG 1 cut(s) 787
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 4 cut(s) 5, 113, 329, 521
RsaNI GTAC 4 cut(s) 4, 112, 328, 520
RseI CAYNNNNRTG 1 cut(s) 140
SaqAI TTAA 2 cut(s) 33, 716
SatI GCNGC 3 cut(s) 90, 783, 786
Sau3AI GATC 1 cut(s) 264
SchI GAGTC 1 cut(s) 315
SduI GDGCHC 1 cut(s) 542
SfaNI GCATC 3 cut(s) 302, 371, 560
SfcI CTRYAG 3 cut(s) 219, 657, 783
SfuI TTCGAA 1 cut(s) 998
SmiMI CAYNNNNRTG 1 cut(s) 140
SphI GCATGC 1 cut(s) 307
Sse9I AATT 8 cut(s) 14, 28, 158, 180, 403, 433, 994, 1032
SspI AATATT 1 cut(s) 805
SspMI CTAG 4 cut(s) 152, 290, 440, 680
TaaI ACNGT 2 cut(s) 111, 943
TaiI ACGT 1 cut(s) 539
TaqI TCGA 4 cut(s) 341, 848, 990, 998
TasI AATT 8 cut(s) 14, 28, 158, 180, 403, 433, 994, 1032
TatI WGTACW 3 cut(s) 3, 111, 519
TfiI GAWTC 3 cut(s) 531, 653, 1042
Tru1I TTAA 2 cut(s) 33, 716
Tru9I TTAA 2 cut(s) 33, 716
TscAI CASTG 2 cut(s) 900, 948
TseFI GTSAC 1 cut(s) 763
TseI GCWGC 3 cut(s) 89, 782, 785
Tsp45I GTSAC 1 cut(s) 763
TspDTI ATGAA 4 cut(s) 237, 467, 722, 957
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 2 cut(s) 900, 948
VneI GTGCAC 1 cut(s) 538
XagI CCTNNNNNAGG 1 cut(s) 584
XapI RAATTY 3 cut(s) 14, 28, 1032
XceI RCATGY 2 cut(s) 10, 307
XmiI GTMKAC 1 cut(s) 57
XspI CTAG 4 cut(s) 152, 290, 440, 680
Zsp2I ATGCAT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.