Rh5AG102100

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0017618
Physical Location & Seq
Forward (+)
9443397 .. 9444801
1405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG102100.1

Sequence Viewer

Length: 1116 bp
ATGGGGAAGGCTCAAGATCTGCAACTCCACATCATAGTTCAAGAAGCTGAACCAGCAATTTCTCAGCCCAGAAATTTTAAGTGGTGGCTCCGTATCGGCGTCTACACACTCTTTGTTCTCTTTGGCCAGTCAGCAGCTATACTCTTGGGACGACTGTACTACAACAAAGGTGGAAAAAGCATATGGATGGCAACCCTAGTGCAATTTGTAGGGTTTCCAATCCTAATTCCTTACCAATGTCTCTCATCATCCAAAAAAATCACTACAGAAAGCAATGTGAACCTGCCATCTTCATCAGTTCTTGCATTGATCTATCTCTCTTTTGGTGTGCTACTAGCATCAGATAGCATGCTGTATTCCATTGGACTCTTGTACCTTCCAGTTTCGACATTTTCACTCATTTGTGCATCTCAGTTGGTCTTCAATGCTTTCTTCTCCTTCTTCCTCAATTCACAAAAGTTCACACCTTACATTGTCAATTCCCTAGTCCTTCTCACCATCTCCTCCATGTTACTTGTGTTTCATGCCAACTCCATAAATGTCGCTGGAGTTTCTAAAGAGAAGTACATAATCGGAATCACGTGCACCATTGGTGCATCTGCTGGCTATGGTTTGATGTTTTCCCTTACGCAGGTAGCTTTCCAGAAAGTTCTAAAATCAAATGCATTTAGTGCTGTGTTAGAAATGATAATCTTTGAATCTATAGTTGCAACCTGTGCTACCCTAGTTGGACTTTTCGTCAGCAAAGAGATGAAGGGGTTAAAGAGAGAGATTGAGGAGTTTGAGCTGGGGAGGCTATTCTACTTGGTGACTTTGGTTTGCACAGCTGCAGCATGGCAAGCTTTCAATATTGGTGCAATAGGACTGGTTTTTGAGGTTTCTTCCTTATTCTCGAATGTTATATGTGTTTCGGGCTTGCCATTTGTTCCAGTTCTCGCAGTGATATTCTTCAATGATAGTCTGGATGGAATAAAGGTTATTGCAACAGTGTTGGCAATCTGGGGATTTGCTTCATATCTCTACCAGCATTATCTCGACAATTCGAACTCCAACCCTGAAACCAAACCTGTAACAGAAATTTCAATGATTCCTTTACTGAAAGAGACAAACTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

371

Amino Acids

40.79

Weight (kDa)

8.07

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 31 - 348 9.2e-114 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g06160
malus_domestica MD05G1289900.v1.1
prunus_persica Prupe.4G075800_v2.0.a1
rosa_laevigata RLG00000031994
rosa_multiflora Rmu_sc0004521.1_g000005
rosa_roxburghii Rroxscaffold_1G00063410
rosa_rugosa Rorug05G0007800
rosa_samantha Rh5AG102100 Rh5BG098400 Rh5CG110600 Rh5DG097700
rosa_wichuraiana Rw5G008910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 291, 622
AccI GTMKAC 1 cut(s) 102
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 2 cut(s) 73, 1077
AcvI CACGTG 1 cut(s) 582
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 3 cut(s) 158, 374, 566
AfiI CCNNNNNNNGG 1 cut(s) 631
AgsI TTSAA 6 cut(s) 41, 424, 698, 847, 952, 1083
AhdI GACNNNNNGTC 1 cut(s) 737
AluBI AGCT 6 cut(s) 47, 137, 638, 787, 827, 842
AluI AGCT 6 cut(s) 47, 137, 638, 787, 827, 842
Alw21I GWGCWC 1 cut(s) 587
Alw26I GTCTC 2 cut(s) 245, 1097
Alw44I GTGCAC 1 cut(s) 583
AoxI GGCC 1 cut(s) 124
ApaLI GTGCAC 1 cut(s) 583
ApeKI GCWGC 3 cut(s) 134, 827, 830
ApoI RAATTY 2 cut(s) 73, 1077
AsuHPI GGTGA 2 cut(s) 487, 820
AsuII TTCGAA 1 cut(s) 1043
BaeGI GKGCMC 1 cut(s) 587
BaeI ACNNNNGTAYC 2 cut(s) 356, 389
BalI TGGCCA 1 cut(s) 126
BbrPI CACGTG 1 cut(s) 582
BbsI GAAGAC 1 cut(s) 412
Bbv12I GWGCWC 1 cut(s) 587
BbvI GCAGC 3 cut(s) 146, 814, 842
BccI CCATC 4 cut(s) 181, 295, 506, 959
BcoDI GTCTC 2 cut(s) 245, 1097
BfaI CTAG 4 cut(s) 197, 335, 485, 725
BfmI CTRYAG 3 cut(s) 264, 702, 828
BfuAI ACCTGC 2 cut(s) 291, 622
BglII AGATCT 1 cut(s) 16
BisI GCNGC 3 cut(s) 135, 828, 831
BlsI GCNGC 3 cut(s) 136, 829, 832
BmeRI GACNNNNNGTC 1 cut(s) 737
BmiI GGNNCC 1 cut(s) 89
BmsI GCATC 3 cut(s) 347, 416, 605
BpiI GAAGAC 1 cut(s) 412
BpmI CTGGAG 1 cut(s) 567
Bpu14I TTCGAA 1 cut(s) 1043
BsaAI YACGTR 1 cut(s) 582
BsaHI GRCGYC 1 cut(s) 99
Bsc4I CCNNNNNNNGG 1 cut(s) 631
Bse1I ACTGG 4 cut(s) 127, 380, 870, 929
Bse3DI GCAATG 1 cut(s) 280
BseGI GGATG 3 cut(s) 192, 248, 970
BseLI CCNNNNNNNGG 1 cut(s) 631
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 2 cut(s) 77, 425
BseNI ACTGG 4 cut(s) 127, 380, 870, 929
BseRI GAGGAG 2 cut(s) 493, 791
BseSI GKGCMC 1 cut(s) 587
BseXI GCAGC 3 cut(s) 146, 814, 842
BseYI CCCAGC 1 cut(s) 787
BshFI GGCC 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 587
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 631
BsmAI GTCTC 2 cut(s) 245, 1097
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 1 cut(s) 126
Bsp119I TTCGAA 1 cut(s) 1043
Bsp1286I GDGCHC 1 cut(s) 587
Bsp143I GATC 2 cut(s) 16, 309
BspANI GGCC 1 cut(s) 126
BspCNI CTCAG 2 cut(s) 76, 424
BspLI GGNNCC 1 cut(s) 89
BspMAI CTGCAG 1 cut(s) 832
BspMI ACCTGC 2 cut(s) 291, 622
BspT104I TTCGAA 1 cut(s) 1043
BsrDI GCAATG 1 cut(s) 280
BsrI ACTGG 4 cut(s) 127, 380, 870, 929
BssMI GATC 2 cut(s) 16, 309
BssNI GRCGYC 1 cut(s) 99
Bst4CI ACNGT 2 cut(s) 156, 988
BstACI GRCGYC 1 cut(s) 99
BstAPI GCANNNNNTGC 2 cut(s) 671, 716
BstBAI YACGTR 1 cut(s) 582
BstBI TTCGAA 1 cut(s) 1043
BstC8I GCNNGC 4 cut(s) 350, 604, 840, 917
BstDEI CTNAG 2 cut(s) 63, 411
BstENI CCTNNNNNAGG 1 cut(s) 629
BstF5I GGATG 3 cut(s) 192, 248, 970
BstKTI GATC 2 cut(s) 19, 312
BstMAI GTCTC 2 cut(s) 245, 1097
BstMBI GATC 2 cut(s) 16, 309
BstMWI GCNNNNNNNGC 5 cut(s) 53, 671, 716, 793, 839
BstNSI RCATGY 1 cut(s) 352
BstSFI CTRYAG 3 cut(s) 264, 702, 828
BstSLI GKGCMC 1 cut(s) 587
BstV1I GCAGC 3 cut(s) 146, 814, 842
BstV2I GAAGAC 1 cut(s) 412
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BsuRI GGCC 1 cut(s) 126
BtsCI GGATG 3 cut(s) 192, 248, 970
BtsI GCAGTG 1 cut(s) 945
BtsIMutI CAGTG 2 cut(s) 945, 993
BveI ACCTGC 2 cut(s) 291, 622
Cac8I GCNNGC 4 cut(s) 350, 604, 840, 917
CseI GACGC 1 cut(s) 88
Csp6I GTAC 3 cut(s) 157, 373, 565
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 4 cut(s) 349, 508, 524, 834
CviQI GTAC 3 cut(s) 157, 373, 565
DdeI CTNAG 2 cut(s) 63, 411
DpnI GATC 2 cut(s) 18, 311
DpnII GATC 2 cut(s) 16, 309
DriI GACNNNNNGTC 1 cut(s) 737
EaeI YGGCCR 1 cut(s) 124
Eam1105I GACNNNNNGTC 1 cut(s) 737
Eco72I CACGTG 1 cut(s) 582
EcoNI CCTNNNNNAGG 1 cut(s) 629
EcoT22I ATGCAT 1 cut(s) 667
FaeI CATG 4 cut(s) 352, 511, 527, 837
FaqI GGGAC 1 cut(s) 162
FatI CATG 4 cut(s) 348, 507, 523, 833
FauNDI CATATG 1 cut(s) 182
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 3 cut(s) 135, 828, 831
FokI GGATG 3 cut(s) 199, 235, 977
Fsp4HI GCNGC 3 cut(s) 135, 828, 831
FspBI CTAG 4 cut(s) 197, 335, 485, 725
GluI GCNGC 3 cut(s) 135, 828, 831
GsaI CCCAGC 1 cut(s) 791
GsuI CTGGAG 1 cut(s) 567
HaeIII GGCC 1 cut(s) 126
HgaI GACGC 1 cut(s) 88
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 4 cut(s) 352, 511, 527, 837
HindIII AAGCTT 1 cut(s) 840
HinfI GANTC 4 cut(s) 366, 576, 698, 1087
HphI GGTGA 2 cut(s) 487, 820
Hpy166II GTNNAC 4 cut(s) 103, 280, 462, 585
Hpy188I TCNGA 2 cut(s) 343, 575
Hpy188III TCNNGA 6 cut(s) 14, 41, 643, 892, 962, 1034
Hpy8I GTNNAC 4 cut(s) 103, 280, 462, 585
HpyAV CCTTC 4 cut(s) 386, 448, 500, 748
HpyCH4III ACNGT 2 cut(s) 156, 988
HpyCH4IV ACGT 1 cut(s) 581
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 671, 716, 793, 839
HpyF3I CTNAG 2 cut(s) 63, 411
HpySE526I ACGT 1 cut(s) 581
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 4 cut(s) 352, 511, 527, 837
Kzo9I GATC 2 cut(s) 16, 309
LmnI GCTCC 1 cut(s) 93
Lsp1109I GCAGC 3 cut(s) 146, 814, 842
LweI GCATC 3 cut(s) 347, 416, 605
MaeI CTAG 4 cut(s) 197, 335, 485, 725
MaeII ACGT 1 cut(s) 581
MaeIII GTNAC 3 cut(s) 510, 808, 1069
MalI GATC 2 cut(s) 18, 311
MboI GATC 2 cut(s) 16, 309
MboII GAAGA 6 cut(s) 282, 412, 424, 433, 873, 940
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 587
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 8 cut(s) 57, 73, 203, 225, 448, 478, 1039, 1077
MluNI TGGCCA 1 cut(s) 126
MlyI GAGTC 1 cut(s) 360
MmeI TCCRAC 2 cut(s) 709, 1074
MnlI CCTC 5 cut(s) 455, 514, 769, 786, 868
Mox20I TGGCCA 1 cut(s) 126
Mph1103I ATGCAT 1 cut(s) 667
MscI TGGCCA 1 cut(s) 126
MseI TTAA 2 cut(s) 78, 761
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 126
MspA1I CMGCKG 1 cut(s) 827
MwoI GCNNNNNNNGC 5 cut(s) 53, 671, 716, 793, 839
NdeI CATATG 1 cut(s) 182
NdeII GATC 2 cut(s) 16, 309
NlaIII CATG 4 cut(s) 352, 511, 527, 837
NlaIV GGNNCC 1 cut(s) 89
NmuCI GTSAC 1 cut(s) 808
NsiI ATGCAT 1 cut(s) 667
NspI RCATGY 1 cut(s) 352
NspV TTCGAA 1 cut(s) 1043
PaeI GCATGC 1 cut(s) 352
PfeI GAWTC 3 cut(s) 576, 698, 1087
PkrI GCNGC 3 cut(s) 136, 829, 832
PleI GAGTC 1 cut(s) 360
PmaCI CACGTG 1 cut(s) 582
PmlI CACGTG 1 cut(s) 582
PpsI GAGTC 1 cut(s) 360
Ppu21I YACGTR 1 cut(s) 582
PspCI CACGTG 1 cut(s) 582
PspFI CCCAGC 1 cut(s) 787
PspN4I GGNNCC 1 cut(s) 89
PstI CTGCAG 1 cut(s) 832
PsuI RGATCY 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 827
RsaI GTAC 3 cut(s) 158, 374, 566
RsaNI GTAC 3 cut(s) 157, 373, 565
RseI CAYNNNNRTG 1 cut(s) 185
SaqAI TTAA 2 cut(s) 78, 761
SatI GCNGC 3 cut(s) 135, 828, 831
Sau3AI GATC 2 cut(s) 16, 309
SchI GAGTC 1 cut(s) 360
SduI GDGCHC 1 cut(s) 587
SfaNI GCATC 3 cut(s) 347, 416, 605
SfcI CTRYAG 3 cut(s) 264, 702, 828
SfuI TTCGAA 1 cut(s) 1043
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
SphI GCATGC 1 cut(s) 352
Sse9I AATT 8 cut(s) 57, 73, 203, 225, 448, 478, 1039, 1077
SspI AATATT 1 cut(s) 850
SspMI CTAG 4 cut(s) 197, 335, 485, 725
TaaI ACNGT 2 cut(s) 156, 988
TaiI ACGT 1 cut(s) 584
TaqI TCGA 4 cut(s) 386, 893, 1035, 1043
TasI AATT 8 cut(s) 57, 73, 203, 225, 448, 478, 1039, 1077
TatI WGTACW 2 cut(s) 156, 564
TfiI GAWTC 3 cut(s) 576, 698, 1087
Tru1I TTAA 2 cut(s) 78, 761
Tru9I TTAA 2 cut(s) 78, 761
TscAI CASTG 2 cut(s) 945, 993
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 3 cut(s) 134, 827, 830
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 4 cut(s) 282, 512, 767, 1002
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 2 cut(s) 945, 993
VneI GTGCAC 1 cut(s) 583
XagI CCTNNNNNAGG 1 cut(s) 629
XapI RAATTY 2 cut(s) 73, 1077
XceI RCATGY 1 cut(s) 352
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 4 cut(s) 197, 335, 485, 725
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.