MD05G1289900.v1.1

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0017618
Physical Location & Seq
Reverse (-)
42188457 .. 42189631
1175 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1289900.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGTGTTTCATACCAGTTAAAGAAGCTGAAGAAGATAATGCACTTGAGTTTAAGAAAACCCCCCACCAAGTAACAACTTCTCAGCCCGGAGATTTTATACGATGGGTGCGTATCACCATCTATACGCTATTCATCCTCTCCGGTCAGTCAGCAGCTATACTCTTGGGCCGACTGTACTATGACGAAGGTGGAAAAATTATATGGATGGCAACACTAGTGCAATTCACAGGGTTTCCTATCCTAATTCCTTACCACTGCATCTTATCATCCAAAAAGATCGCCGCACATAGCAATGTGCACCAACCATCTGCCTCAGTACTGACAGCGATGAATCTCTCTCTTGGTGTATTACTCGCAGCAGACAGCTTGTTGTATTCTATGGGACTCTTGTACCTTCCAGTTTCTACCTTTTCACTCATTTGTGCATCGCAGTTGGCCTTCAATGCTTTCTTCTCGGTCTGCCTTAATTCACAAAAGTTCACTCCTTTTATAGTCAATTCTCTAGTCCTTCTCACCATCTCCTCCACCCTGCTTGTGTTTCACCCCAACTCAGCAAACCTCACAGGAGTCTCCAAAGAGAGGTATACGATTGGGGTCATTTGCACTGTTGGTGCATCAGCTGGGTACGGCTTGATGTTCTCCCTCACACAGTTCTCCTTCGAAAAGGTTCTAAAAGCAAAAACTTTTGTAACTGTATTGGAAATGATAATCTACGAATCTCTAATTGCAACCTGTGCTACACTAGTGGGACTTTTTGTCACTGGAGAATGGAAGGGTTTAAAGAGCGAGATGGAGGAGTTTGCGTTGGGGAGGCTATCCTATTTCCTGACTTTGATTTGTACAGCTACAGCATGTCAAACTTTCAACATTGGCGCCATAGGATTGATATTTGAGGTGTCTTCTTTAGTCTCCAATGTCATAAGTGCTTCCGGCTTACCTTTCATTCCAGTTCTAGCTGTAATCTTCTTCAATGATAACATGGATGGAATAAAGGTGATTGCCACGTTGTTGGCCCTCTGGGGCTTTGCGTCATATCTCTATCAGCACTACCTTGACGATTCAGAGTCCAAATCTGAAACTAAACATGTAGAAGAAATTTCAATGATTCCTTTACTTAAAGAGGTCAACTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

378

Amino Acids

41.37

Weight (kDa)

5.82

Isoelectric Point (pI)

30.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 37 - 354 1.7e-113 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017618)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g06160
malus_domestica MD05G1289900.v1.1
prunus_persica Prupe.4G075800_v2.0.a1
rosa_laevigata RLG00000031994
rosa_multiflora Rmu_sc0004521.1_g000005
rosa_roxburghii Rroxscaffold_1G00063410
rosa_rugosa Rorug05G0007800
rosa_samantha Rh5AG102100 Rh5BG098400 Rh5CG110600 Rh5DG097700
rosa_wichuraiana Rw5G008910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 872
AccI GTMKAC 1 cut(s) 584
AciI CCGC 1 cut(s) 282
AcsI RAATTY 1 cut(s) 1095
AcuI CTGAAG 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 873
AfaI GTAC 5 cut(s) 176, 318, 392, 626, 841
AfiI CCNNNNNNNGG 1 cut(s) 579
AflIII ACRYGT 1 cut(s) 1084
AgsI TTSAA 4 cut(s) 442, 865, 970, 1101
AhdI GACNNNNNGTC 1 cut(s) 755
AhlI ACTAGT 2 cut(s) 214, 742
AluBI AGCT 6 cut(s) 26, 155, 366, 620, 845, 956
AluI AGCT 6 cut(s) 26, 155, 366, 620, 845, 956
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 2 cut(s) 574, 913
Alw44I GTGCAC 1 cut(s) 296
AoxI GGCC 3 cut(s) 166, 435, 1011
ApaLI GTGCAC 1 cut(s) 296
ApeKI GCWGC 2 cut(s) 152, 356
ApoI RAATTY 1 cut(s) 1095
Asp700I GAANNNNTTC 1 cut(s) 666
AspLEI GCGC 1 cut(s) 875
AspS9I GGNCC 2 cut(s) 166, 1012
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 4 cut(s) 106, 505, 533, 1006
AsuII TTCGAA 1 cut(s) 660
BaeGI GKGCMC 1 cut(s) 300
BaeI ACNNNNGTAYC 2 cut(s) 374, 407
BanI GGYRCC 1 cut(s) 872
BbsI GAAGAC 1 cut(s) 891
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 2 cut(s) 164, 368
BccI CCATC 7 cut(s) 96, 125, 199, 313, 524, 784, 977
BceAI ACGGC 1 cut(s) 643
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 2 cut(s) 574, 913
BcuI ACTAGT 2 cut(s) 214, 742
BfaI CTAG 4 cut(s) 215, 503, 743, 953
BfmI CTRYAG 1 cut(s) 846
BfoI RGCGCY 1 cut(s) 876
BisI GCNGC 3 cut(s) 153, 282, 357
BlsI GCNGC 3 cut(s) 154, 283, 358
BmcAI AGTACT 1 cut(s) 318
Bme1390I CCNGG 1 cut(s) 87
BmeRI GACNNNNNGTC 1 cut(s) 755
BmgT120I GGNCC 2 cut(s) 166, 1012
BmiI GGNNCC 1 cut(s) 874
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 3 cut(s) 267, 434, 623
BpiI GAAGAC 1 cut(s) 891
BpmI CTGGAG 1 cut(s) 783
Bpu14I TTCGAA 1 cut(s) 660
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 87
BsaHI GRCGYC 1 cut(s) 873
BsaWI WCCGGW 1 cut(s) 140
BsaXI ACNNNNNCTCC 2 cut(s) 788, 818
Bsc4I CCNNNNNNNGG 1 cut(s) 579
Bse1I ACTGG 4 cut(s) 14, 398, 766, 947
Bse3DI GCAATG 1 cut(s) 298
BseGI GGATG 4 cut(s) 132, 210, 266, 988
BseLI CCNNNNNNNGG 1 cut(s) 579
BseMI GCAATG 1 cut(s) 298
BseMII CTCAG 3 cut(s) 95, 327, 564
BseNI ACTGG 4 cut(s) 14, 398, 766, 947
BseRI GAGGAG 2 cut(s) 511, 809
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 2 cut(s) 164, 368
BseYI CCCAGC 1 cut(s) 620
BshFI GGCC 3 cut(s) 168, 437, 1013
BshNI GGYRCC 1 cut(s) 872
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 3 cut(s) 87, 141, 930
BslFI GGGAC 2 cut(s) 396, 762
BslI CCNNNNNNNGG 1 cut(s) 579
BsmAI GTCTC 2 cut(s) 574, 913
BsmFI GGGAC 2 cut(s) 396, 762
BsnI GGCC 3 cut(s) 168, 437, 1013
Bsp119I TTCGAA 1 cut(s) 660
Bsp1286I GDGCHC 1 cut(s) 300
Bsp1407I TGTACA 1 cut(s) 839
Bsp143I GATC 1 cut(s) 276
BspACI CCGC 1 cut(s) 282
BspANI GGCC 3 cut(s) 168, 437, 1013
BspCNI CTCAG 3 cut(s) 94, 326, 563
BspLI GGNNCC 1 cut(s) 874
BspT104I TTCGAA 1 cut(s) 660
BspT107I GGYRCC 1 cut(s) 872
BsrDI GCAATG 1 cut(s) 298
BsrGI TGTACA 1 cut(s) 839
BsrI ACTGG 4 cut(s) 14, 398, 766, 947
BssMI GATC 1 cut(s) 276
BssNAI GTATAC 1 cut(s) 585
BssNI GRCGYC 1 cut(s) 873
Bst1107I GTATAC 1 cut(s) 585
Bst4CI ACNGT 4 cut(s) 174, 607, 651, 694
BstACI GRCGYC 1 cut(s) 873
BstAPI GCANNNNNTGC 1 cut(s) 734
BstAUI TGTACA 1 cut(s) 839
BstBI TTCGAA 1 cut(s) 660
BstDEI CTNAG 3 cut(s) 81, 313, 550
BstF5I GGATG 4 cut(s) 132, 210, 266, 988
BstH2I RGCGCY 1 cut(s) 876
BstHHI GCGC 1 cut(s) 875
BstKTI GATC 1 cut(s) 279
BstMAI GTCTC 2 cut(s) 574, 913
BstMBI GATC 1 cut(s) 276
BstMWI GCNNNNNNNGC 2 cut(s) 443, 734
BstNSI RCATGY 2 cut(s) 855, 1088
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 846
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 164, 368
BstV2I GAAGAC 1 cut(s) 891
BstXI CCANNNNNNTGG 1 cut(s) 1009
BstZ17I GTATAC 1 cut(s) 585
BsuRI GGCC 3 cut(s) 168, 437, 1013
BtgZI GCGATG 2 cut(s) 341, 411
BtsCI GGATG 4 cut(s) 132, 210, 266, 988
BtsI GCAGTG 1 cut(s) 253
BtsIMutI CAGTG 3 cut(s) 253, 603, 759
CfoI GCGC 1 cut(s) 875
Cfr13I GGNCC 2 cut(s) 166, 1012
CseI GACGC 1 cut(s) 1017
Csp6I GTAC 5 cut(s) 175, 317, 391, 625, 840
CspCI CAANNNNNGTGG 2 cut(s) 514, 549
CviAII CATG 3 cut(s) 852, 979, 1085
CviQI GTAC 5 cut(s) 175, 317, 391, 625, 840
DdeI CTNAG 3 cut(s) 81, 313, 550
DinI GGCGCC 1 cut(s) 874
DpnI GATC 1 cut(s) 278
DpnII GATC 1 cut(s) 276
DraI TTTAAA 1 cut(s) 780
DriI GACNNNNNGTC 1 cut(s) 755
Eam1105I GACNNNNNGTC 1 cut(s) 755
Eco57I CTGAAG 1 cut(s) 48
EgeI GGCGCC 1 cut(s) 874
EheI GGCGCC 1 cut(s) 874
FaeI CATG 3 cut(s) 855, 982, 1088
FaqI GGGAC 2 cut(s) 396, 762
FatI CATG 3 cut(s) 851, 978, 1084
FblI GTMKAC 1 cut(s) 584
Fnu4HI GCNGC 3 cut(s) 153, 282, 357
FokI GGATG 4 cut(s) 119, 217, 253, 995
Fsp4HI GCNGC 3 cut(s) 153, 282, 357
FspBI CTAG 4 cut(s) 215, 503, 743, 953
GlaI GCGC 1 cut(s) 874
GluI GCNGC 3 cut(s) 153, 282, 357
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 1 cut(s) 783
HaeII RGCGCY 1 cut(s) 876
HaeIII GGCC 3 cut(s) 168, 437, 1013
HapII CCGG 3 cut(s) 87, 141, 930
HgaI GACGC 1 cut(s) 1017
HhaI GCGC 1 cut(s) 875
Hin1I GRCGYC 1 cut(s) 873
Hin1II CATG 3 cut(s) 855, 982, 1088
Hin6I GCGC 1 cut(s) 873
HinP1I GCGC 1 cut(s) 873
HincII GTYRAC 1 cut(s) 1126
HindII GTYRAC 1 cut(s) 1126
HinfI GANTC 7 cut(s) 331, 384, 567, 716, 1058, 1064, 1105
HpaII CCGG 3 cut(s) 87, 141, 930
HphI GGTGA 4 cut(s) 106, 505, 533, 1006
Hpy166II GTNNAC 4 cut(s) 298, 480, 585, 1126
Hpy188I TCNGA 2 cut(s) 1063, 1075
Hpy188III TCNNGA 1 cut(s) 826
Hpy8I GTNNAC 4 cut(s) 298, 480, 585, 1126
HpyAV CCTTC 6 cut(s) 179, 404, 448, 518, 667, 766
HpyCH4III ACNGT 4 cut(s) 174, 607, 651, 694
HpyCH4IV ACGT 1 cut(s) 1004
HpyCH4V TGCA 8 cut(s) 41, 220, 258, 298, 425, 603, 614, 728
HpyF10VI GCNNNNNNNGC 2 cut(s) 443, 734
HpyF3I CTNAG 3 cut(s) 81, 313, 550
HpySE526I ACGT 1 cut(s) 1004
Hsp92I GRCGYC 1 cut(s) 873
Hsp92II CATG 3 cut(s) 855, 982, 1088
HspAI GCGC 1 cut(s) 873
KasI GGCGCC 1 cut(s) 872
Kzo9I GATC 1 cut(s) 276
Lsp1109I GCAGC 2 cut(s) 164, 368
LweI GCATC 3 cut(s) 267, 434, 623
MaeI CTAG 4 cut(s) 215, 503, 743, 953
MaeII ACGT 1 cut(s) 1004
MaeIII GTNAC 3 cut(s) 70, 688, 757
MalI GATC 1 cut(s) 278
MboI GATC 1 cut(s) 276
MboII GAAGA 7 cut(s) 41, 44, 442, 891, 955, 958, 1103
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 7 cut(s) 195, 221, 243, 466, 496, 723, 1095
Mly113I GGCGCC 1 cut(s) 873
MlyI GAGTC 3 cut(s) 378, 576, 1073
MroXI GAANNNNTTC 1 cut(s) 666
MseI TTAA 5 cut(s) 18, 51, 465, 779, 1116
MslI CAYNNNNRTG 1 cut(s) 291
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 3 cut(s) 87, 141, 930
MspR9I CCNGG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 443, 734
NarI GGCGCC 1 cut(s) 873
NciI CCSGG 1 cut(s) 87
NdeII GATC 1 cut(s) 276
NlaIII CATG 3 cut(s) 855, 982, 1088
NlaIV GGNNCC 1 cut(s) 874
NmuCI GTSAC 1 cut(s) 757
NspI RCATGY 2 cut(s) 855, 1088
NspV TTCGAA 1 cut(s) 660
PciI ACATGT 1 cut(s) 1084
PcsI WCGNNNNNNNCGW 1 cut(s) 106
PdmI GAANNNNTTC 1 cut(s) 666
PfeI GAWTC 4 cut(s) 331, 716, 1058, 1105
PkrI GCNGC 3 cut(s) 154, 283, 358
PleI GAGTC 3 cut(s) 378, 575, 1072
PluTI GGCGCC 1 cut(s) 876
PpsI GAGTC 3 cut(s) 378, 575, 1072
PscI ACATGT 1 cut(s) 1084
PspFI CCCAGC 1 cut(s) 620
PspN4I GGNNCC 1 cut(s) 874
PspPI GGNCC 2 cut(s) 166, 1012
PvuII CAGCTG 1 cut(s) 620
RsaI GTAC 5 cut(s) 176, 318, 392, 626, 841
RsaNI GTAC 5 cut(s) 175, 317, 391, 625, 840
RseI CAYNNNNRTG 1 cut(s) 291
SaqAI TTAA 5 cut(s) 18, 51, 465, 779, 1116
SatI GCNGC 3 cut(s) 153, 282, 357
Sau3AI GATC 1 cut(s) 276
Sau96I GGNCC 2 cut(s) 166, 1012
ScaI AGTACT 1 cut(s) 318
SchI GAGTC 3 cut(s) 378, 576, 1073
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 300
SfaNI GCATC 3 cut(s) 267, 434, 623
SfcI CTRYAG 1 cut(s) 846
SfoI GGCGCC 1 cut(s) 874
SfuI TTCGAA 1 cut(s) 660
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SpeI ACTAGT 2 cut(s) 214, 742
Sse9I AATT 7 cut(s) 195, 221, 243, 466, 496, 723, 1095
SsiI CCGC 1 cut(s) 282
SspDI GGCGCC 1 cut(s) 872
SspMI CTAG 4 cut(s) 215, 503, 743, 953
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 4 cut(s) 174, 607, 651, 694
TaiI ACGT 1 cut(s) 1007
TaqI TCGA 1 cut(s) 660
TaqII GACCGA 1 cut(s) 445
TasI AATT 7 cut(s) 195, 221, 243, 466, 496, 723, 1095
TatI WGTACW 3 cut(s) 174, 316, 839
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 4 cut(s) 331, 716, 1058, 1105
Tru1I TTAA 5 cut(s) 18, 51, 465, 779, 1116
Tru9I TTAA 5 cut(s) 18, 51, 465, 779, 1116
TscAI CASTG 3 cut(s) 260, 610, 766
TseFI GTSAC 1 cut(s) 757
TseI GCWGC 2 cut(s) 152, 356
Tsp45I GTSAC 1 cut(s) 757
TspDTI ATGAA 3 cut(s) 121, 344, 931
TspRI CASTG 3 cut(s) 260, 610, 766
VneI GTGCAC 1 cut(s) 296
XapI RAATTY 1 cut(s) 1095
XceI RCATGY 2 cut(s) 855, 1088
XmiI GTMKAC 1 cut(s) 584
XmnI GAANNNNTTC 1 cut(s) 666
XspI CTAG 4 cut(s) 215, 503, 743, 953
ZrmI AGTACT 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.