FvH4_3g09321

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0020154
Physical Location & Seq
Reverse (-)
5445819 .. 5448470
2652 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09321.t1

Sequence Viewer

Length: 303 bp
ATGGCCAAAGAAATTGGAAGAAGAAGGAGGAGGAGGAGGAAGCATAATTCCAAATTTCCAATGAGTCCCCAAGATAAGGACCGGCGGCTAAAGAGGTTATCGCGGATGGTCCTACATGTTGACTCGTGCAAGAATGCGCCGATCAAGAAGGCTACGCCGACAGGGAGAAGGTTACGCCGGAGATCTCCGTCGATCTTCTGCTCCGACCTAACCCTAGGCTCACCCATATCTAACTCACGAAGGCGACGTCAACATGCTGAAGGCTACACCGAAGATCTCCGCCGATCTTCTGCTTCGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

11.77

Weight (kDa)

11.93

Isoelectric Point (pI)

147.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020154)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27740
fragaria_vesca FvH4_3g09321
malus_domestica MD12G1019800.v1.1
rosa_samantha Rh3BG202800 Rh3CG193100 Rh3CG200300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 250
AccII CGCG 1 cut(s) 103
AciI CCGC 3 cut(s) 85, 103, 280
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 53
AcuI CTGAAG 1 cut(s) 279
AcyI GRCGYC 1 cut(s) 247
AfiI CCNNNNNNNGG 1 cut(s) 76
AflIII ACRYGT 1 cut(s) 115
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 1 cut(s) 53
ArsI GACNNNNNNTTYG 2 cut(s) 232, 264
AspA2I CCTAGG 1 cut(s) 214
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 2 cut(s) 79, 109
AsuHPI GGTGA 1 cut(s) 213
AvaII GGWCC 2 cut(s) 79, 109
AvrII CCTAGG 1 cut(s) 214
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 124
BccI CCATC 1 cut(s) 100
BfaI CTAG 1 cut(s) 215
BglII AGATCT 2 cut(s) 182, 274
BisI GCNGC 1 cut(s) 86
BlnI CCTAGG 1 cut(s) 214
BlsI GCNGC 1 cut(s) 87
Bme18I GGWCC 2 cut(s) 79, 109
BmgT120I GGNCC 2 cut(s) 79, 109
BsaHI GRCGYC 1 cut(s) 247
BsaJI CCNNGG 1 cut(s) 214
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse118I RCCGGY 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 76
BseRI GAGGAG 3 cut(s) 43, 46, 49
Bsh1236I CGCG 1 cut(s) 103
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 2 cut(s) 82, 178
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 51
BsmI GAATGC 1 cut(s) 139
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 6 cut(s) 141, 182, 192, 274, 284, 297
BspACI CCGC 3 cut(s) 85, 103, 280
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 1 cut(s) 103
BsrFI RCCGGY 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 6 cut(s) 141, 182, 192, 274, 284, 297
BssNI GRCGYC 1 cut(s) 247
BssSI CACGAG 1 cut(s) 124
BssT1I CCWWGG 1 cut(s) 214
Bst2BI CACGAG 1 cut(s) 124
BstACI GRCGYC 1 cut(s) 247
BstF5I GGATG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 103
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 6 cut(s) 144, 185, 195, 277, 287, 300
BstMBI GATC 6 cut(s) 141, 182, 192, 274, 284, 297
BstNSI RCATGY 2 cut(s) 119, 257
BstUI CGCG 1 cut(s) 103
BstX2I RGATCY 2 cut(s) 182, 274
BstYI RGATCY 2 cut(s) 182, 274
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 111
CfoI GCGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 81
Cfr13I GGNCC 2 cut(s) 79, 109
CviAII CATG 2 cut(s) 116, 254
CviJI RGCY 5 cut(s) 5, 88, 152, 219, 264
CviKI_1 RGCY 5 cut(s) 5, 88, 152, 219, 264
DpnI GATC 6 cut(s) 143, 184, 194, 276, 286, 299
DpnII GATC 6 cut(s) 141, 182, 192, 274, 284, 297
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 269
Eco130I CCWWGG 1 cut(s) 214
Eco47I GGWCC 2 cut(s) 79, 109
Eco57I CTGAAG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 214
FaeI CATG 2 cut(s) 119, 257
FaiI YATR 4 cut(s) 45, 117, 227, 255
FaqI GGGAC 1 cut(s) 51
FatI CATG 2 cut(s) 115, 253
Fnu4HI GCNGC 1 cut(s) 86
FokI GGATG 1 cut(s) 118
Fsp4HI GCNGC 1 cut(s) 86
FspBI CTAG 1 cut(s) 215
GlaI GCGC 1 cut(s) 138
GluI GCNGC 1 cut(s) 86
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 82, 178
HhaI GCGC 1 cut(s) 139
Hin1I GRCGYC 1 cut(s) 247
Hin1II CATG 2 cut(s) 119, 257
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 2 cut(s) 121, 251
HindII GTYRAC 2 cut(s) 121, 251
HinfI GANTC 2 cut(s) 64, 122
HpaII CCGG 2 cut(s) 82, 178
HphI GGTGA 1 cut(s) 213
Hpy166II GTNNAC 2 cut(s) 121, 251
Hpy188I TCNGA 1 cut(s) 205
Hpy188III TCNNGA 2 cut(s) 145, 237
Hpy8I GTNNAC 2 cut(s) 121, 251
Hpy99I CGWCG 2 cut(s) 193, 249
HpyAV CCTTC 5 cut(s) 18, 142, 162, 234, 254
HpyCH4IV ACGT 1 cut(s) 247
HpyCH4V TGCA 1 cut(s) 129
HpySE526I ACGT 1 cut(s) 247
Hsp92I GRCGYC 1 cut(s) 247
Hsp92II CATG 2 cut(s) 119, 257
HspAI GCGC 1 cut(s) 137
Kzo9I GATC 6 cut(s) 141, 182, 192, 274, 284, 297
LmnI GCTCC 1 cut(s) 206
LpnPI CCDG 3 cut(s) 95, 147, 191
MaeI CTAG 1 cut(s) 215
MaeII ACGT 1 cut(s) 247
MaeIII GTNAC 1 cut(s) 171
MalI GATC 6 cut(s) 143, 184, 194, 276, 286, 299
MboI GATC 6 cut(s) 141, 182, 192, 274, 284, 297
MboII GAAGA 5 cut(s) 30, 33, 187, 279, 284
MflI RGATCY 2 cut(s) 182, 274
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 12, 46, 53
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 73, 116
MmeI TCCRAC 1 cut(s) 228
MnlI CCTC 5 cut(s) 21, 24, 27, 30, 87
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 82, 178
Mva1269I GAATGC 1 cut(s) 139
MvnI CGCG 1 cut(s) 103
NdeII GATC 6 cut(s) 141, 182, 192, 274, 284, 297
NlaIII CATG 2 cut(s) 119, 257
NspI RCATGY 2 cut(s) 119, 257
PciI ACATGT 1 cut(s) 115
PcsI WCGNNNNNNNCGW 1 cut(s) 244
PctI GAATGC 1 cut(s) 139
PkrI GCNGC 1 cut(s) 87
PleI GAGTC 2 cut(s) 72, 116
PpsI GAGTC 2 cut(s) 72, 116
PscI ACATGT 1 cut(s) 115
PspPI GGNCC 2 cut(s) 79, 109
PsuI RGATCY 2 cut(s) 182, 274
SatI GCNGC 1 cut(s) 86
Sau3AI GATC 6 cut(s) 141, 182, 192, 274, 284, 297
Sau96I GGNCC 2 cut(s) 79, 109
SchI GAGTC 2 cut(s) 73, 116
SetI ASST 4 cut(s) 98, 173, 210, 250
SinI GGWCC 2 cut(s) 79, 109
Sse9I AATT 3 cut(s) 12, 46, 53
SsiI CCGC 3 cut(s) 85, 103, 280
SspMI CTAG 1 cut(s) 215
StyI CCWWGG 1 cut(s) 214
TaiI ACGT 1 cut(s) 250
TaqI TCGA 2 cut(s) 191, 296
TasI AATT 3 cut(s) 12, 46, 53
TauI GCSGC 1 cut(s) 88
TspGWI ACGGA 1 cut(s) 177
VpaK11BI GGWCC 2 cut(s) 79, 109
XapI RAATTY 1 cut(s) 53
XceI RCATGY 2 cut(s) 119, 257
XmaJI CCTAGG 1 cut(s) 214
XspI CTAG 1 cut(s) 215
ZraI GACGTC 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.